CN1392241A - Biological reactor - Google Patents
Biological reactor Download PDFInfo
- Publication number
- CN1392241A CN1392241A CN 02138795 CN02138795A CN1392241A CN 1392241 A CN1392241 A CN 1392241A CN 02138795 CN02138795 CN 02138795 CN 02138795 A CN02138795 A CN 02138795A CN 1392241 A CN1392241 A CN 1392241A
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- CN
- China
- Prior art keywords
- magnetic cylinder
- top cover
- bearing
- bio
- reactor
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/20—Material Coatings
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/34—Internal compartments or partitions
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
- C12M27/08—Stirrer or mobile mixing elements with different stirrer shapes in one shaft or axis
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Sustainable Development (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Immunology (AREA)
Abstract
The bioreactor includes a cylinder and a transmission mechanism and has top cover to seal the cylinder, cup-shaped outer magnetic tank connected to the motor shaft, coaxial inner magnetic tank, stirring shaft with one end fixed to the inner tank and the other end extending beyond the top cover, and positioning bearing for the stirring shaft and inside a bearing box fixed to the top cover. Both the outer and inner magnetic tanks are made of permanent magnetic material while the outer parts of non-magnetic material. Compared with available technology, the present invention has the advantages of sealed cylinder, complete sterilization, easy bearing installation etc.
Description
Technical field
The present invention relates to a kind of bio-reactor, is a kind of bio-reactor that utilizes the magnetic force coupling to drive the stir shaft rotation.
Background technology
At present, bio-reactor is widely used in animal and plant cell cultures and microbial fermentation field, is biotechnology industry core production unit.In implementing bio-reactor large scale culturing process, microbiological contamination is a very serious problem, often cause whole bioreactor culture thing and raw material pollution, scrap, cause the tremendous economic loss.The whipping appts of existing bio-reactor needs the outer electric motor contact of stir shaft and tank body to be connected to transmit mechanical movement, axle passes cover, adopt mechanical seal between axle and the cover, as Chinese utility model patent 97222109.3 disclosed a kind of " sealing of fermentor tank special machinery ".Therefore, life-time service can cause an envelope place to cause poorly sealedly because of wearing and tearing, and assorted bacterium is entered in the reactor, pollutes culture.
Summary of the invention
The object of the present invention is to provide a kind of bio-reactor that can overcome above-mentioned defective, can prevent effectively that assorted bacterium from entering in the reactor.
For achieving the above object, bio-reactor of the present invention comprises tank body and transmission mechanism, it is characterized in that:
The axle top cover is fixed on the upper cover plate of tank body and makes the tank body sealing, and its outside is with the outer magnetic cylinder of the cup-shaped that links to each other with motor shaft, is positioned at a top cover with the interior magnetic cylinder of the coaxial setting of outer magnetic cylinder, does not contact mutually between outer magnetic cylinder, a top cover and the interior magnetic cylinder; One end of stir shaft is fixedlyed connected with interior magnetic cylinder, and the medium pore that the other end passes upper cover plate places in the tank body, leaves the space between stir shaft and this medium pore; The locating bearing that is provided with on the stir shaft places in the plumer block, and plumer block is fixed on the upper cover plate by the plumer block retaining plate, leaves the space between plumer block retaining plate and the upper cover plate; Outer magnetic cylinder and interior magnetic cylinder are made by permanent magnet material, and remaining part is made by non-magnet material.
Bio-reactor tank body provided by the invention seals fully, and the stir shaft that utilizes coaxial-type magnetic force coupled type of drive to drive in the reactor sealing cover rotates.Because do not have the axle envelope, overcome the microbiological contamination problem of bringing because of mechanical seal; This device can be used as various types of medium and small bio-reactors and microorganism fermentation tank, and can realize easily being connected with polytype stir shaft.Particularly, the utility model compared with prior art has the following advantages:
1. adopt the whipping appts of magnetic coupling transmission, tank body adopts static seal, isolates fully with the external world, is difficult for microbiological contamination, helps realizing the long pure culture of animal and plant cell and microorganism.
2. adopt unique stir shaft anchor design, inner no dead angle, the thorough sterilization at magnetic cylinder position in the very easy realization.
3. bearing is easy for installation, adopts sliding surface bearing can solve the bearing rust problem.
4 is easy to maintenance, saves man-hour.
Description of drawings
Fig. 1 is the structural representation of bio-reactor of the present invention;
Fig. 2 is the structural representation of transmission mechanism among the present invention;
Fig. 3 is the structural representation that the stir shaft of single direction thrust ball bearing and sliding surface bearing is housed;
Fig. 4 is the structural representation that the stir shaft of 2 sliding surface bearing is housed.
Embodiment
The invention will be further described below in conjunction with drawings and Examples.
As shown in Figure 1, magnetic coupling transmission stirring type bioreactor mainly is made of motor shaft 1, outer magnetic cylinder 2, axle top cover 3, interior magnetic cylinder 4, interior magnetic cylinder standing bolt 7, reactor upper cover plate 8, stir shaft 9, plumer block retaining plate 11, inlet pipe 14, flange 15, glass outer wall 16, temperature sensor jack 17, chuck 18, pull bar 19, discharge nozzle 20 and stirring rake 21.Wherein, it is emphasized that, reactor upper cover plate 8 central authorities should reserve certain hole around stir shaft 9, and should leave certain space between plumer block retaining plate 11 and the reactor upper cover plate 8, high pressure steam can enter in the top cover 3 by hole when making sterilization, thereby thoroughly kills assorted bacterium.Other interface arrangement on the reactor (as vapor pipe, inoculated tube, feed supplement pipe and stopple coupon etc.) and detection control apparatus (as rotating speed, pH, dissolved oxygen, the isoparametric detection of temperature and control device etc.) do not illustrate in the drawings.
Fig. 2 is the structure iron of transmission mechanism, mainly is made of motor shaft 1, outer magnetic cylinder 2, axle top cover 3, interior magnetic cylinder 4, top cover standing bolt 5, seal washer 6, interior magnetic cylinder standing bolt 7, reactor upper cover plate 8 and stir shaft 9.Outer magnetic cylinder 2 is a cup-like structure, links to each other with motor shaft 1, is suspended on a top cover 3 outsides, and is coaxial with the interior magnetic cylinder 4 of inside; Interior magnetic cylinder 4 is fixed in stir shaft 9 tops.Magnetic cylinder 4 and stir shaft 9 co-rotation in just can driving simultaneously when outer magnetic cylinder 2 rotates under driven by motor.Axle top cover 3 is made by the stainless material of non-magnetic conduction, so can not influence the magnetic coupling interaction between inside and outside magnetic cylinder.Should note in order to avoid increase friction, reducing transmission efficiency, loss equipment during outer magnetic cylinder 2 location not with axle top cover 3 outer walls and contact with platform.When outer magnetic cylinder 2 highly equated with interior magnetic cylinder 4, technique effect was better.
The locating bearing that connects with axle can have multiple different form, and shown in Figure 3 is the stir shaft that a single direction thrust ball bearing 13 and a sliding surface bearing 10 are housed, and shown in Figure 4 is the stir shaft that 2 sliding surface bearing 10 are housed.Stir shaft 9 and plumer block 12 are fixed on the reactor upper cover plate 8 by plumer block retaining plate 11, thus with interior magnetic cylinder 4 location, and can carry out maintain and replace to stir shaft and bearing easily, save man-hour; And all bearings sliding surface bearing that can adopt tetrafluoroethylene to be processed into, can solve the plain bearing problem of rustiness.
From above-mentioned explanation as can be seen, essence of the present utility model is the magnetic force coupling transmission device is used for bio-reactor, and therefore, the employed reactor tank body of the utility model is not limited to above-mentioned concrete structure.
Claims (4)
1. bio-reactor, comprise tank body and transmission mechanism, it is characterized in that: axle top cover (3) is fixed on the upper cover plate (8) of tank body, and tank body is sealed, its outside is with the outer magnetic cylinder (2) of the cup-shaped that links to each other with motor shaft (1), be positioned at a top cover (3) with the interior magnetic cylinder (4) of the coaxial setting of outer magnetic cylinder (2), do not contact mutually between outer magnetic cylinder (2), axle top cover (3) and the interior magnetic cylinder (4); One end of stir shaft (9) is fixedlyed connected with interior magnetic cylinder (4), and the medium pore that the other end passes upper cover plate (8) places in the tank body, leaves the space between stir shaft (9) and this medium pore; Stir shaft (9) is gone up the locating bearing that is provided with and is placed in the plumer block (12), and plumer block (12) is fixed on the upper cover plate (8) by plumer block retaining plate (11), leaves the space between plumer block retaining plate (11) and the upper cover plate (8); Outer magnetic cylinder (2) and interior magnetic cylinder (4) are made by permanent magnet material, and remaining part is made by non-magnet material.
2. bio-reactor according to claim 1 is characterized in that: magnetic cylinder (4) is highly equal with outer magnetic cylinder (2) in described.
3. bio-reactor according to claim 1 and 2 is characterized in that: described locating bearing is made of a single direction thrust ball bearing (13) and a sliding surface bearing (10).
4. bio-reactor according to claim 1 and 2 is characterized in that: described locating bearing is made of two sliding surface bearing (10).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB021387958A CN1171989C (en) | 2002-07-13 | 2002-07-13 | Biological reactor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB021387958A CN1171989C (en) | 2002-07-13 | 2002-07-13 | Biological reactor |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1392241A true CN1392241A (en) | 2003-01-22 |
| CN1171989C CN1171989C (en) | 2004-10-20 |
Family
ID=4749715
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB021387958A Expired - Fee Related CN1171989C (en) | 2002-07-13 | 2002-07-13 | Biological reactor |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1171989C (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101797489A (en) * | 2010-03-20 | 2010-08-11 | 浙江长城减速机有限公司 | Magnetic auxiliary support for kettle |
| CN104379722A (en) * | 2012-06-15 | 2015-02-25 | 艾本德有限公司 | Single-use bioreactor and head plate, and a process for manufacturing same |
| CN105974066A (en) * | 2016-06-16 | 2016-09-28 | 湖南中本智能科技发展有限公司 | Vertical type animal gastrointestinal tract bionic digestion device |
-
2002
- 2002-07-13 CN CNB021387958A patent/CN1171989C/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101797489A (en) * | 2010-03-20 | 2010-08-11 | 浙江长城减速机有限公司 | Magnetic auxiliary support for kettle |
| CN101797489B (en) * | 2010-03-20 | 2012-07-18 | 浙江长城减速机有限公司 | Magnetic auxiliary support for kettle |
| CN104379722A (en) * | 2012-06-15 | 2015-02-25 | 艾本德有限公司 | Single-use bioreactor and head plate, and a process for manufacturing same |
| US10030220B2 (en) | 2012-06-15 | 2018-07-24 | Eppendorf Ag | Single-use bioreactor and head plate, and a process for manufacturing same |
| CN105974066A (en) * | 2016-06-16 | 2016-09-28 | 湖南中本智能科技发展有限公司 | Vertical type animal gastrointestinal tract bionic digestion device |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1171989C (en) | 2004-10-20 |
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| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |