CN110244006A - A kind of vertical gastrointestinal tract simulation digest tube of anti-enzyme solution wall built-up - Google Patents
A kind of vertical gastrointestinal tract simulation digest tube of anti-enzyme solution wall built-up Download PDFInfo
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- CN110244006A CN110244006A CN201910619323.1A CN201910619323A CN110244006A CN 110244006 A CN110244006 A CN 110244006A CN 201910619323 A CN201910619323 A CN 201910619323A CN 110244006 A CN110244006 A CN 110244006A
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Abstract
The invention discloses a kind of anti-vertical gastrointestinal tracts of enzyme solution wall built-up to simulate digest tube, including vertical glass tube, glass inner tube is equipped in vertical glass tube, dustproof cover is plugged at the top of glass inner tube, dustproof cover is equipped with perforation, the bottom of glass inner tube is protruded into vertical glass tube, its top is stretched out outside vertical glass tube, the top position that glass inner tube stretches out vertical glass tube is equipped with enzyme interface tube, enzyme pipe is arranged in enzyme interface tube, one end that enzyme pipe is located in enzyme interface tube is arranged with drainage tube, drainage tube includes horizontal part and bending part, horizontal part is connect with enzyme pipe, and its one end far from enzyme pipe bends downward to form bending part along the direction of glass inner tube, bending part lower end extends in glass inner tube.The phenomenon that enzyme solution is drained in glass inner tube using the bending part of drainage tube, is avoided enzyme solution wall built-up by the present invention guarantees that enzyme solution participates in the enzyme liquid amount of digestion reaction, and Feed Sample is made completely, to be improved digestion rate by abundant digestion and be digested the precision of result.
Description
Technical field
The present invention relates to Bionic digestion technical field of experiment equipment, in particular to a kind of vertical gastrointestinal tract mould of anti-enzyme solution wall built-up
Paragastric canals.
Background technique
In modern farming, the objective nutrient biological value for accurately evaluating feedstuff is that determining Animal nutrition needs
Measure and optimize the primary decision-making basis of feed formula.From the 1960s, people are attempting to pass through conditions in vitro always
Digestion of the Imitating feed in animal gastrointestinal tract is to realize the rapid evaluation to feed nutrient biological value.
Triangular flask or test tube are generallyd use in the prior art as gastrointestinal tract Bionic digestion device.Carry out simulation digestion experiment
When, first the feed of constant weight is fitted into the triangular flask or test tube being installed on rack for test tube, then into triangular flask or test tube
Enzyme solution or buffer is added and is placed on water bath with thermostatic control shaking table and vibrates, is driven in digestion process by the motor above rack for test tube
Dynamic that invisible spectro agitating shaft is driven to be stirred Feed Sample and digestive juice, this process is to simulate the digestion of animal stomach or intestines
Process, after digesting, centrifuged or filtered again goes out oneself and digests substance and undigested material, when the weight of undigested material
When weight relative to Feed Sample is higher, indicate that digestibility is low.
CN200920105937.X discloses a kind of Simulative digestive device for gastrointestinal tract of animals, it includes glass tube and dialysis tubing,
The glass tube is a hollow tube, and the both ends of the tube body are respectively provided with a ground, and the side of the tube body is equipped with input pipe and efferent duct,
The dialysis tubing is placed in the glass tube, and the both ends of the dialysis tubing are stretched out from the two of the glass tube grounds respectively and turned up,
The dialysis tube end being exposed on the outside of ground that turns up is tied up by rubber item to be fixed on ground, two mills after tying up dialysis tube end
Mouth is plugged with a sealing-plug and one respectively and has the sealing-plug of perfusion tube.
CN201610424205.1 discloses a kind of vertical animal gastrointestinal tract Bionic digestion device, including motor, connecting bushing,
Drive rod, agitating shaft and simulation digest tube is turned up；The simulation digest tube is the hollow vertical glass tube of pair of lamina；It is described vertical
Glass tube by a hollow tube, be set in the intracorporal glass inner tube of hollow tube and enzyme pipe forms, the hollow tube upper and lower side
It is respectively provided with a ground, is plugged with sealing-plug in ground；Hollow tube side wall lower ends and upper end are respectively equipped with input pipe and efferent duct；Institute
Enzyme pipe is stated across hollow tube side wall and is extended in glass inner tube, hollow tube inner space is separated into outer by glass inner tube
Cavity and content cavity.
The above patent is all made of device of the glass tube as gastrointestinal tract Bionic digestion, by perfusion tube or enzyme pipe to dialysis
Input stomach or small intestine digestive juice (i.e. enzyme solution) digest feed in dialysis tubing or glass inner tube in pipe or glass inner tube, but by
In in digestion reaction enzyme solution dosage it is less, enzyme solution is directly conveyed by dialysis tubing or glass inner tube, will lead to enzyme solution downward
There is wall built-up phenomenon in tube wall in flow process adhesion, so that the enzyme liquid amount for flowing into dialysis tubing or glass inner tube bottom is reduced,
Lead to Feed Sample digestion not exclusively, influence digestion effect and digests the accuracy of result.
Summary of the invention
The technical problem to be solved by the present invention is overcoming the deficiencies of the prior art and provide, a kind of structure is simple, user
Just, it can be avoided the vertical gastrointestinal tract simulation digest tube of enzyme solution wall built-up phenomenon in digestion reaction.
The technical solution adopted by the present invention to solve the technical problems is: a kind of vertical gastrointestinal tract simulation of anti-enzyme solution wall built-up disappears
Change pipe, including vertical glass tube, is equipped with glass inner tube in the vertical glass tube, the glass inner tube will be in vertical glass tube
Portion space is divided into outer cavity and content cavity, and dustproof cover is plugged at the top of the glass inner tube, and the dustproof cover is equipped with and is used for
The perforation of agitating shaft is worn, the bottom of the glass inner tube is protruded into the vertical glass tube, and the vertical glass is stretched out at top
Outside glass pipe, the top position that the glass inner tube stretches out the vertical glass tube is equipped with enzyme interface tube, the enzyme interface tube
It is inside arranged with enzyme pipe, one end that the enzyme pipe is located in the enzyme interface tube is arranged with drainage tube, the drainage tube packet
Horizontal part and bending part are included, the horizontal part is connect with the enzyme pipe, and its one end far from the enzyme pipe is along the glass
The direction of glass inner tube bends downward to form the bending part, and the bending part lower end extends in glass inner tube.
Enzyme solution is drained in glass inner tube out of enzyme pipe by the bending part of drainage tube, enzyme solution is avoided and sticks at glass
The phenomenon that inner pipe wall causes it to participate in the decline of digestion reaction enzyme liquid amount makes the Feed Sample of glass inner tube bottom by sufficiently completely
Digestion is decomposed, and is improved digestion reaction rate and is digested the accuracy of result.
Further, the bottom of the bending part is located in the glass inner tube more than the liquid level of sample liquid.
Further, the bottom of the bending part is between the glass inner tube and the agitating shaft, and itself and the glass
Glass inner tube and the agitating shaft do not contact.
Further, the length of the bending part is 60-70mm.
The length of drainage tube is set as 60-70mm, if drainage length of tube is too long, enzyme solution flowing time in drainage tube is too long
The speed that enzyme solution addition can be reduced causes digestion reaction rate to decline；If it is too short to drain length of tube, flowed out from the draft tube bottom
The distance between Feed Sample of the enzyme solution from glass inner tube bottom farther out, when it is downwardly towards glass inner tube bottom, to feeding
Expecting that the impact force of sample is larger will lead on the inner wall that Feed Sample is broken up to glass inner tube, and Feed Sample is made to be adhered to glass
Inner wall above glass inner tube causes digestion reaction result inaccurate, influences to disappear to reduce the sample size for participating in digestion reaction
Change effect.
Further, the vertical glass tube side wall lower ends and upper end are respectively equipped with input interface tube and output interface tube.
Further, the glass inner tube bottom is arc-shaped.
Further, the dustproof cover includes ring plug and cylindrical plugs, the lower surface of the ring plug and the cylinder
The upper surface of plug connects, and it is integrally formed with the cylindrical plugs.
Further, the cylindrical plugs are filled in the glass inner tube, and the depth filled in the glass inner tube is less than
The distance between at the top of the top of the glass inner tube and the enzyme interface.
A kind of vertical gastrointestinal tract simulation digest tube of anti-enzyme solution wall built-up of the present invention the utility model has the advantages that structure of the invention it is simple,
It is easy to use, by being arranged with drainage tube in enzyme pipe, enzyme solution is drained in glass inner tube using the bending part of drainage tube,
So that enzyme solution is flowed into glass inner tube bottom from drainage tube, enzyme solution is prevented to be adhered to glass inner tube inner wall and from glass inner tube inner wall to glass
The phenomenon that glass bottom of inner tube flows down, the phenomenon that avoiding enzyme solution wall built-up, promote enzyme solution smoothly to flow into glass from enzyme pipe
Bottom of inner tube guarantees that enzyme solution participates in the enzyme liquid amount of digestion reaction, mentions the Feed Sample in glass inner tube completely, by abundant digestion
The high precision and stability of digestion rate and digestion result.
Detailed description of the invention
Fig. 1-is the schematic perspective view that a kind of anti-vertical gastrointestinal tract of enzyme solution wall built-up of the present invention simulates digest tube；
Fig. 2-is the cross-sectional view in Fig. 1 at A-A.
Above-mentioned appended drawing reference: the vertical glass tube of 1-, 2- glass inner tube, 3- dustproof cover, 4- perforation, the enzyme interface tube of 5-, 6-
Enzyme pipe, 7- input interface tube, and 8- exports interface tube, 9- drainage tube, 10- content cavity, the outer cavity of 11-, 12- ring plug, 13- circle
Cylindrical plag.
Specific embodiment
The invention will be further described with reference to the accompanying drawings and embodiments, but these specific embodiments are not with any side
Formula limits the scope of the invention.
Embodiment 1
A kind of vertical gastrointestinal tract of anti-enzyme solution wall built-up simulates digest tube, including vertical glass tube 1, is equipped in the vertical glass tube 1
Glass inner tube 2, the inner space of vertical glass tube 1 is divided into outer cavity 11 and content cavity 10 by the glass inner tube 2, described perpendicular
1 side wall lower ends of straight glass tube and upper end are respectively equipped with input interface tube 7 and output interface tube 8, the top plug of the glass inner tube 2
There is dustproof cover 3, the dustproof cover 3 is equipped with the perforation 4 for wearing agitating shaft, and the bottom of the glass inner tube 2 is protruded into described
In vertical glass tube 1, top is stretched out outside the vertical glass tube 1, and the glass inner tube 2 stretches out the vertical glass tube 1
Top position is equipped with enzyme interface tube 5, and the quantity of enzyme interface tube 5 is 2, can input stomach simulation by 2 enzyme interface tubes 5
Digestive juice stomach function regulating buffer is arranged with enzyme pipe 6 in the enzyme interface tube 5, and the enzyme pipe 6 is located at the enzyme interface tube
One end in 5 is arranged with drainage tube 9, and the drainage tube 9 includes horizontal part and bending part, the horizontal part and the enzyme pipe 6
Connection, and its one end far from the enzyme pipe 6 bends downward to form the bending part along the direction of the glass inner tube 2,
The angle of bending is 90 degree, and the bending part lower end extends in glass inner tube 2, and the length of the bending part is 65mm.
The phenomenon that in order to solve enzyme solution wall built-up, the present invention is by being arranged drainage tube 9 in one end of enzyme pipe 6, using drainage
Enzyme solution is drained to 2 bottom of glass inner tube by the drainage of pipe 9 out of enzyme pipe 6, to avoid enzyme solution adhesion in glass inner tube 2
Inner wall causes the phenomenon that participating in the enzyme amount decline of digestion reaction, and enzyme solution can smoothly flow into 2 bottom of glass inner tube for Feed Sample
Sufficiently digestion completely, improves the Stability and veracity of digestion reaction；And enzyme solution is drained to by the bending part of drainage tube 9
In glass inner tube 2, the phenomenon that enzyme solution flows into its bottom along 2 inner wall of glass inner tube and causes enzyme solution wall built-up also can avoid, improve
The efficiency of digestion reaction.
The bottom of the bending part is located in the glass inner tube 2 more than the liquid level of sample liquid, i.e. the bending part of drainage tube
Bottom is not contacted in the ullage of sample liquid with sample liquid, be can avoid 9 bottom of drainage tube and is stretched in Feed Sample and cause to drain
9 bottom of pipe is contaminated, and generation the phenomenon that Feed Sample can be prevented by back suction to drainage tube 9 and be recycled to enzyme pipe 6.
The bottom of the bending part is between the glass inner tube 2 and the agitating shaft, and itself and the glass inner tube 2
It is not contacted with the agitating shaft, can avoid leading to glass bar and drainage due to the CONTACT WITH FRICTION of 9 bottom of drainage tube and agitating shaft
The phenomenon that pipe 9 damages extends the service life of agitating shaft and drainage tube 9, while also can avoid enzyme solution and being stained in glass inner tube 2
Wall promotes going on smoothly for digestion reaction.
1 side wall lower ends of vertical glass tube and upper end are respectively equipped with input interface tube 7 and output interface tube 8.
2 bottom of glass inner tube is arc-shaped.
The dustproof cover 3 includes ring plug 12 and cylindrical plugs 13, lower surface and the cylinder of the ring plug 12
The upper surface connection of plug 13, and it is integrally formed with the cylindrical plugs 13.
The cylindrical plugs 13 are filled in the glass inner tube 2, and fill in the depth in the glass inner tube 2 less than described
The distance between at the top of the top of glass inner tube 2 and the enzyme interface.
A kind of operation principle and using method of the anti-vertical gastrointestinal tract simulation digest tube of enzyme solution wall built-up of the present invention: work of the present invention
When making, by Feed Sample loaded in glass inner tube 2, stomach simulated digestive juice stomach function regulating buffer is by enzyme pipe 6 and in drainage tube 9
It is injected in glass inner tube 2 under drainage；Then the input interface tube 7 for simulating digest tube and output interface tube 8 are separately connected
Input pipe and efferent duct, then input pipe and efferent duct are connected to heat preservation hot-water circulation pipe, hot water is via input pipe from input pipe
It is injected in outer cavity 11 at interface 7, then flows out outer cavity 11 via efferent duct, it is ensured that the digestion reaction in glass inner tube 2 exists
It is carried out under required temperature；Agitating shaft is inserted into realization pair in glass inner tube 2 from the perforation 4 on ring plug 12 and cylindrical plugs 13 again
Feed Sample and digestive juice are stirred, and digestion reaction occurs for Feed Sample, to its digestion reaction after take out drainage tube
9, digestive juice is filtered through enzyme pipe 6 or artificial be transferred in the simulation digest tube for be cased with bag filter, produces the digestion in bag filter
Stomach buffer outside object and bag filter carries out mass exchange, completes stomach and simulates digestion process.
When the simulation for needing to carry out small intestine site digests, above-mentioned stomach simulated digestive juice is changed to small intestine simulation digestion
Liquid, stomach buffer are changed to small intestine buffer, and the case where wriggled according to digesta into duodenum, and setting small intestine simulation digestion phase stirs
The revolving speed of axis is mixed, other operations simulate digestion process with stomach, and then complete the simulation digestion of small intestine site.
When the simulation for needing to be carried out continuously stomach and small intestine digests, stomach simulation digestion process is completed in vertical glass tube 1
After operation, by adding phosphate buffer, stomach phase digestion environment pH is set to be changed into small intestine digestion phase digestive environments pH, and same
When be adjusted to the ion concentration of small intestine digestive juice, then by enzyme pipe 6 by small intestine simulated digestive juice implantation glass inner tube 2,
And the case where being wriggled according to digesta into duodenum, the revolving speed of the agitating shaft of setting small intestine simulation digestion phase continue small intestine simulation
Digestion process operation is completed and then is transferred in the simulation digest tube with bag filter to dialyse, simulates nutrient wait digest
Absorption.
The above described is only a preferred embodiment of the present invention, be not intended to limit the present invention in any form, though
So the present invention has been disclosed as a preferred embodiment, and however, it is not intended to limit the invention, any technology people for being familiar with this profession
Member, without departing from the scope of the present invention, when the technology contents using the disclosure above make a little change or modification
It is right according to the technical essence of the invention for the equivalent embodiment of equivalent variations, but without departing from the technical solutions of the present invention
Any simple modification, equivalent change and modification made by above embodiments, all of which are still within the scope of the technical scheme of the invention.
Claims (8)
1. a kind of vertical gastrointestinal tract of anti-enzyme solution wall built-up simulates digest tube, including vertical glass tube, it is equipped in the vertical glass tube
The inner space of vertical glass tube is divided into outer cavity and content cavity, the glass inner tube by glass inner tube, the glass inner tube
Top be plugged with dustproof cover, the dustproof cover is equipped with the perforation for wearing agitating shaft, it is characterised in that: the glass inner tube
Bottom protrude into the vertical glass tube, top is stretched out outside the vertical glass tube, and the glass inner tube is stretched out described perpendicular
The top position of straight glass tube is equipped with enzyme interface tube, is arranged with enzyme pipe in the enzyme interface tube, the enzyme pipe is located at
One end in the enzyme interface tube is arranged with drainage tube, and the drainage tube includes horizontal part and bending part, the horizontal part with
The enzyme pipe connection, and its one end far from the enzyme pipe bent downward along the direction of the glass inner tube to be formed it is described curved
Folding part, the bending part lower end extend in glass inner tube.
2. a kind of vertical gastrointestinal tract of anti-enzyme solution wall built-up simulates digest tube as described in claim 1, it is characterised in that: the bending part
Bottom be located in the glass inner tube more than the liquid level of sample liquid.
3. a kind of vertical gastrointestinal tract of anti-enzyme solution wall built-up simulates digest tube as described in claim 1, it is characterised in that: the bending part
Bottom between the glass inner tube and the agitating shaft, and it does not connect with the glass inner tube and the agitating shaft
Touching.
4. a kind of vertical gastrointestinal tract of anti-enzyme solution wall built-up simulates digest tube as described in claim 1, it is characterised in that: the bending part
Length be 60-70mm.
5. a kind of vertical gastrointestinal tract of anti-enzyme solution wall built-up simulates digest tube as described in claim 1, it is characterised in that: the vertical glass
Glass pipe side wall lower ends and upper end are respectively equipped with input interface tube and output interface tube.
6. a kind of vertical gastrointestinal tract of anti-enzyme solution wall built-up simulates digest tube as described in claim 1, it is characterised in that: in the glass
Bottom of the tube is arc-shaped.
7. a kind of anti-vertical gastrointestinal tract simulation digest tube of enzyme solution wall built-up as described in claim any one of 1-6, it is characterised in that: institute
Stating dustproof cover includes ring plug and cylindrical plugs, and the lower surface of the ring plug is connect with the upper surface of the cylindrical plugs, and
It is integrally formed with the cylindrical plugs.
8. a kind of vertical gastrointestinal tract of anti-enzyme solution wall built-up simulates digest tube as claimed in claim 7, it is characterised in that: the cylinder
It fills in the glass inner tube, and fills in the depth in the glass inner tube and add less than the top of the glass inner tube with described
The distance between at the top of enzyme interface.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910619323.1A CN110244006A (en) | 2019-07-10 | 2019-07-10 | A kind of vertical gastrointestinal tract simulation digest tube of anti-enzyme solution wall built-up |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910619323.1A CN110244006A (en) | 2019-07-10 | 2019-07-10 | A kind of vertical gastrointestinal tract simulation digest tube of anti-enzyme solution wall built-up |
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| CN110244006A true CN110244006A (en) | 2019-09-17 |
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| CN201344874Y (en) * | 2009-02-18 | 2009-11-11 | 中国农业科学院北京畜牧兽医研究所 | Simulative digestive device for gastrointestinal tract of animals |
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| CN105842407A (en) * | 2016-06-16 | 2016-08-10 | 湖南中本智能科技发展有限公司 | Vertical type animal gastrointestinal bionic digester |
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| CN205659691U (en) * | 2016-05-23 | 2016-10-26 | 石河子大学 | Device is dissolved surely to accuracy |
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| CN206599573U (en) * | 2017-02-28 | 2017-10-31 | 湖北洛克泰克仪器有限公司 | A kind of reaction unit for being used to simulate animal rumens fermentation |
| CN207079234U (en) * | 2017-07-19 | 2018-03-09 | 许柯 | Simulation rumen slaking apparatus is used in one kind experiment |
| CN207717742U (en) * | 2018-01-03 | 2018-08-10 | 中国海洋大学 | A kind of simulation slaking apparatus of food allergen |
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2019
- 2019-07-10 CN CN201910619323.1A patent/CN110244006A/en active Pending
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201344874Y (en) * | 2009-02-18 | 2009-11-11 | 中国农业科学院北京畜牧兽医研究所 | Simulative digestive device for gastrointestinal tract of animals |
| CN103555575A (en) * | 2013-11-04 | 2014-02-05 | 深圳市赛特罗生物医疗技术有限公司 | Cell culture liquid addition and cell recovery and transfer device |
| CN104851346A (en) * | 2015-04-30 | 2015-08-19 | 暨南大学 | Modular animal digestive tract in-vitro simulation system and human intestinal tract simulation method thereof |
| CN205659691U (en) * | 2016-05-23 | 2016-10-26 | 石河子大学 | Device is dissolved surely to accuracy |
| CN105842407A (en) * | 2016-06-16 | 2016-08-10 | 湖南中本智能科技发展有限公司 | Vertical type animal gastrointestinal bionic digester |
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| CN206095885U (en) * | 2016-10-11 | 2017-04-12 | 山东科技大学 | Novel specific surface analysis appearance sample cell advance appearance device |
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