CN201344926Y

CN201344926Y - Bionic digestive system for monogastric animals

Info

Publication number: CN201344926Y
Application number: CNU2009201059365U
Authority: CN
Inventors: 赵峰; 张宏福; 卢庆萍; 胡光源; 张子仪
Original assignee: Institute of Animal Science of CAAS
Current assignee: Institute of Animal Science of CAAS
Priority date: 2009-02-18
Filing date: 2009-02-18
Publication date: 2009-11-11
Anticipated expiration: 2019-02-18

Abstract

The utility model discloses a bionic digestive system for monogastric animals. The system is characterized in that at least one simulative digestive organ is arranged on a fixed claw of an air bath shaking table; at least one digestive juice reagent bottle is connected with an infusion tube; a main peristaltic pump is connected with an input pipe; a waste solution containing bottle is connected with the main peristaltic pump; at least one buffer solution reagent bottle is connected with the main peristaltic pump as well as with an output pipe; a cleaning solution reagent bottle is connected with the main peristaltic pump as well as with the output pipe and a cleaning solution storage bottle; the cleaning solution reagent bottle is connected with a cleaning residual solution containing bottle at the lower part; and the output pipe is communicated with or isolated from air through a communicating control electromagnetic valve. The bionic digestive system for monogastric animals of the utility model achieves the purpose that the process of feed digestive absorption in gastrointestinal tract of an animal can be simulated in vitro; the simulation verisimilitude is high; and the repeatability is good; in addition, the system of the utility model has the advantages of simple structure and convenient operation, and is particularly suitable for quick measurement of the biological value of feed nutrition for monogastric animals such as pigs, chickens, ducks and the like.

Description

The monogastric animal bionic digestive system

Technical field

The utility model relates to a kind of monogastric animal bionic digestive system, specifically, and the bionical digestive system that relate to a kind of simulation feed quilt digestion in the nonruminant intestines and stomach under conditions in vitro, absorbs.

Background technology

In modern farming, the objective nutrient biological value of accurately evaluating feedstuff is the primary decision-making basis of determining the Animal nutrition requirement and optimizing feed formula.Under the situation, if need many decades at least with the biological value of the whole general surveys of animal experiment method, prediction feed nutrient, and the result only can obtain very limited static parameter to of a great variety, the renewal speed of feedstuff faster in China.Therefore, in order to break through the technical bottleneck of restriction China feed nutrient biological value parameter update, design a kind of science, feed nutrient biological value testing tool is badly in need of accurately and rapidly, it can be modern farming technical support is provided.

From the sixties in 20th century, in order to realize the rapid evaluation to the feed nutrient biological value, the various countries scholar is studying the simulation digestion techniques of feed nutrient always, and has set up a series of normalized working specifications.For example, the middle and later periods in 20th century, Chinese scholar Zhang Ziyi etc. have set up with pepsin---and intestinal fluid is pig, the chicken feed effective energy value zymetology assessment method working specification of system; Denmark scholar Boisen and Fernandez have set up that---pancreatin---carbohydrase is the pig feed digestible energy value rapid method for assessment working specification of system with pepsin; It is pig, the chicken feed amino acid digestibility rapid method for assessment of system that Canada scholar Gauther etc., Savoie etc. have set up with the proteinase dialysis.Yet, the intestines and stomach simulation slaking apparatus that these methods adopted is many based on simple experiment materials such as triangular flask, test tube, dialysis tubings, in each step digestion process, the separation of the change of pH value, the adding of digestive juice, product etc. all is that the mode by manually realizes, therefore, this class simple analog digestive system repeatable relatively poor, the maximum deviation of parallel sample reaches more than 4%, and, the precision of its mensuration mainly is the regression correction that relies on mathematical model, and this system is breeding bigger randomness and instability.Along with development of science and technology, the technical bottleneck that these methods have also become the development of animal and fowl fodder nutrient biological value rapid method for assessment and used.

Compare with early stage animal gastrointestinal tract simulation digestive system, the bionical digestive system of pig all-digestive tract that Dutch TNO food and nutrient research institute developed in nineteen ninety-five comprises stomach, duodenum, jejunum, four continuous analog digestion chambers of ileum.The absorption of wriggling, secretion of digestive juice and small intestine of emptying, the small intestine of stomach can be simulated by this system.In addition, though Canadian food research and centre of development in the bionical digestive system of human epimere stomach and intestine and the Dutch TNO food and the nutrient research of development in 2005 design differ greatly, both are comparatively similar on function.As seen, bionical digestive system of new generation is a starting point with the high fidelity of digestion process all, and particularly bionical digestion process all is to realize automatic control by computing machine.Yet the design original intention of these bionical digestive systems is the research that is used for aspects such as intestines and stomach growth of microorganism, pharmacology metabolism, does not measure fodder energy, protein, amino acid whose digestibility and be suitable for.This mainly is because the alimentary canal of this class bionic device is long, and feed is to circulate successively in order between each section alimentary canal, is easy to form the obstruction of feed in a certain section bionical alimentary canal, residual.On design theory, these systems can not satisfy the theory of " all-in and all-out " in the feed nutrient biological value mensuration.

In sum, design a kind of can the realistic simulation feed in animal gastrointestinal tract, be to need the problem of solution at present badly by the technical scheme of digestion, absorption process.

The utility model content

The purpose of this utility model is to provide a kind of monogastric animal bionic digestive system, and under conditions in vitro, but this system's realistic simulation feed is digested in animal gastrointestinal tract, the process of absorption.

In order to achieve the above object, the utility model has adopted following technical scheme:

A kind of monogastric animal bionic digestive system, it is characterized in that: it comprises the simulation digester, the digestive juice reagent bottle, the damping fluid reagent bottle, waste liquid is accommodated bottle, the cleaning fluid storage bottle, cleaning fluid reagent bottle and the residual liquid of cleaning are accommodated bottle, wherein: at least one simulation digester is placed on the interior fixed claw of an air bath shaking table, the port of at least one digestive juice reagent bottle is via carrying peristaltic pump to be connected with the woven hose of this simulation digester with himself corresponding digestive juice, first input/output port of one direct peristaltic pump is connected with the input pipe of this simulation digester, the port that one waste liquid is accommodated bottle is connected with second input/output port of this direct peristaltic pump via an input control electromagnetic valve, the port that is placed at least one damping fluid reagent bottle in the thermostatic water-circulator bath groove is via being connected with second input/output port of this direct peristaltic pump with himself corresponding output control electromagnetic valve, the port of this at least one damping fluid reagent bottle via with himself corresponding one the input control electromagnetic valve be connected with the efferent duct of this simulation digester, the port of one cleaning fluid reagent bottle is connected with second input/output port of this direct peristaltic pump via an output control electromagnetic valve, the port of this cleaning fluid reagent bottle is respectively via an input control electromagnetic valve, one cleaning fluid is carried the efferent duct of peristaltic pump and this simulation digester, the port of one cleaning fluid storage bottle connects, the below of this cleaning fluid reagent bottle is placed the residual liquid of a cleaning and is accommodated bottle, this cleans residual liquid and accommodates the opening that the port of bottle offers bottom this cleaning fluid reagent bottle via an input control electromagnetic valve and be connected, the efferent duct of this simulation digester communicates via one that control electromagnetic valve communicates with extraneous air or is isolated, the control end of this control electromagnetic valve that communicates, the control end of all input control electromagnetic valve, the control end of all output control electromagnetic valve, the control end of this air bath shaking table, the control end of this thermostatic water-circulator bath groove is connected with the control end of a Programmable Logic Controller, the control end of this direct peristaltic pump, all digestive juices are carried the control end of peristaltic pump, this cleaning fluid is carried the control end of peristaltic pump, the communication terminal of this Programmable Logic Controller is connected with the communication terminal of CPU.

During actual design, the quantity of described digestive juice reagent bottle and described damping fluid reagent bottle can be for individual arbitrarily.For example, the quantity of described digestive juice reagent bottle and described damping fluid reagent bottle is one, described digestive juice reagent bottle and described damping fluid reagent bottle be splendid attire peptic digest liquid, stomach damping fluid respectively, perhaps described digestive juice reagent bottle and described damping fluid reagent bottle be splendid attire small intestine digestive juice, small intestine damping fluid respectively, and perhaps described digestive juice reagent bottle and described damping fluid reagent bottle be splendid attire large intestine digestive juice, large intestine damping fluid respectively.Again for example, the quantity of described digestive juice reagent bottle and described damping fluid reagent bottle is two, a described digestive juice reagent bottle and a described damping fluid reagent bottle are one group, wherein: described digestive juice reagent bottle in a group and described damping fluid reagent bottle be splendid attire peptic digest liquid, stomach damping fluid respectively, and described digestive juice reagent bottle in another group and described damping fluid reagent bottle be splendid attire small intestine digestive juice, small intestine damping fluid respectively; Perhaps, described digestive juice reagent bottle in a group and described damping fluid reagent bottle be splendid attire small intestine digestive juice, small intestine damping fluid respectively, and described digestive juice reagent bottle in another group and described damping fluid reagent bottle be splendid attire large intestine digestive juice, large intestine damping fluid respectively; Perhaps, described digestive juice reagent bottle in a group and described damping fluid reagent bottle be splendid attire peptic digest liquid, stomach damping fluid respectively, and described digestive juice reagent bottle in another group and described damping fluid reagent bottle be splendid attire large intestine digestive juice, large intestine damping fluid respectively.Again for example, the quantity of described digestive juice reagent bottle and described damping fluid reagent bottle is three, a described digestive juice reagent bottle and a described damping fluid reagent bottle are one group, wherein: described digestive juice reagent bottle in first group and described damping fluid reagent bottle be splendid attire peptic digest liquid, stomach damping fluid respectively, described digestive juice reagent bottle in second group and described damping fluid reagent bottle be splendid attire small intestine digestive juice, small intestine damping fluid respectively, and described digestive juice reagent bottle in the 3rd group and described damping fluid reagent bottle be splendid attire large intestine digestive juice, large intestine damping fluid respectively.Again for example, the quantity of described digestive juice reagent bottle is two, the quantity of described damping fluid reagent bottle is three, wherein: two described digestive juice reagent bottles are distinguished splendid attire small intestine digestive juice, large intestine digestive juice, and three described damping fluid reagent bottles are distinguished splendid attire stomach damping fluid, small intestine damping fluid, large intestine damping fluid.

In addition, in the actual design, the quantity of described simulation digester can be two or more, between the input pipe of each described simulation digester, the efferent duct for being connected in series, between the woven hose of each described simulation digester for being connected in parallel; Described simulation digester comprises glass tube and dialysis tubing, this glass tube is a hollow tube, the two ends of this body respectively are provided with a ground, the side of this body is provided with described input pipe and described efferent duct, this dialysis tubing is placed in this glass tube, the two ends of this dialysis tubing are stretched out and are turned up from two grounds of this glass tube respectively, the turn up dialysis tubing end that is exposed to the ground outside is tied up by the rubber bar and is fixed on the ground, and two grounds tying up behind the dialysis tubing end are plugged with the rubber plug that a rubber plug and has described woven hose respectively.

The utlity model has following advantage:

1, the utility model monogastric animal bionic digestive system has realized that the simulation feed is digested under conditions in vitro, the process of absorption in animal gastrointestinal tract, whole simulation process is controlled automatically by computer and is finished, but unmanned, got rid of interference from human factor, the reliability height, simulation verisimilitude height, the simulation repeatability is good.The utility model system architecture is simple, and is easy to operate, is specially adapted to the fast measuring of monogastric animal feed nutrient biological values such as pig, chicken, duck.

2, digestive juice by injecting different alimentary canal sections such as stomach, duodenum, jejunum, ileum, large intestine, small intestine for example in dialysis tubing and corresponding change feed the damping fluid in the glass tube, the utility model can be implemented in the situation that the simulation feed is digested and absorbs under the conditions in vitro in each section of animal intestines and stomach alimentary canal, for example realize the simulation to nonruminant stomach, small intestine and big intestinal digestion, absorption process.

3, the utility model system not only can simulate separately respectively digestion, the absorption process of stomach, small intestine and the large intestine of nonruminant, can also carry out continuous analog to digestion, the absorption process of stomach, small intestine and the large intestine of nonruminant, this continuous uninterrupted analog form can be avoided taking place chyme and residue problem occur in shifting.

4, series connection successively between the input and output pipe of respectively simulating digester in the utility model system, this series system have guaranteed that the electrochemical conditions in all simulation digesters is consistent, thereby have reduced simulation error, have improved simulation precision.

5, because the dialysis tubing in the utility model system is tied up with the rubber bar with the position that contacts of glass tube and with turned welt rubber plug jam-pack, therefore, the container of the carrying liquid that dialysis tubing and glass tube constitute respectively is mutually independently, separate, the digestion product of damping fluid and generation is in glass tube respectively, in these two different containers of dialysis tubing, mass exchange between them must be undertaken by the molecular sieve on the dialysis tubing, so, the utility model system can avoid taking place directly to carry out between damping fluid and digestion product because of dialysis tubing is poorly sealed the phenomenon of mass exchange, has guaranteed GI accuracy of simulated animal and verisimilitude.And, because digestion product can't return dialysis tubing after flowing into glass tube via the molecular sieve on the dialysis tubing, dialysis tubing can be proceeded other digestion reactions, therefore, this independent design of dialysis tubing and two containers of glass tube can effectively reduce the inhibition of digestion product to digestion reaction in the dialysis tubing, accurately distinguish digestion material and undigested material, help making the GI digestion process of simulated animal to approach virtual condition more.

6, when using the utility model system, each input pipe, efferent duct of simulating digester lays respectively at below, top, such placement design had both helped the circulation of damping fluid, and helping digesting the damping fluid that remains in the glass tube after the end again can be by thorough emptying.

7, in the utility model, by the temperature and the flow velocity of control damping fluid, can accurately control the interior temperature of dialysis tubing of simulation digester indirectly, thereby the temperature variations amplitude of bionical digestion process is remained in 0.4 ℃, more near the range of variation of animal heat, simulate effect is good.Because a ground of the simulation digester in the utility model system turned welt rubber plug jam-pack that connects L type woven hose, therefore, L type woven hose can make the simulation digestive juice stablize, inject in the dialysis tubing continuously via L type woven hose.

Description of drawings

Fig. 1 is that first embodiment of the utility model monogastric animal bionic digestive system forms synoptic diagram;

Fig. 2 is that second embodiment of the utility model monogastric animal bionic digestive system forms synoptic diagram;

Fig. 3 is the structural representation of the simulation digester in the utility model monogastric animal bionic digestive system;

Fig. 4 is the outline synoptic diagram of the glass tube of simulation digester;

Fig. 5 is the A-A cross-sectional schematic of Fig. 4.

Embodiment

Below in conjunction with accompanying drawing the utility model is described in detail.

The utility model monogastric animal bionic digestive system comprises the simulation digester, the digestive juice reagent bottle, the damping fluid reagent bottle, waste liquid is accommodated bottle, the cleaning fluid storage bottle, cleaning fluid reagent bottle and the residual liquid of cleaning are accommodated bottle, wherein: at least one simulation digester is placed on the interior fixed claw of an air bath shaking table, the port of at least one digestive juice reagent bottle is via carrying peristaltic pump to be connected with the woven hose of this simulation digester with himself corresponding digestive juice, first input/output port of one direct peristaltic pump is connected with the input pipe of this simulation digester, the port that one waste liquid is accommodated bottle is connected with second input/output port of this direct peristaltic pump via an input control electromagnetic valve, the port that is placed at least one damping fluid reagent bottle in the thermostatic water-circulator bath groove is via being connected with second input/output port of this direct peristaltic pump with himself corresponding output control electromagnetic valve, the port of this at least one damping fluid reagent bottle via with himself corresponding one the input control electromagnetic valve be connected with the efferent duct of this simulation digester, the port of one cleaning fluid reagent bottle is connected with second input/output port of this direct peristaltic pump via an output control electromagnetic valve, the port of this cleaning fluid reagent bottle is respectively via an input control electromagnetic valve, one cleaning fluid is carried the efferent duct of peristaltic pump and this simulation digester, the port of one cleaning fluid storage bottle connects, the below of this cleaning fluid reagent bottle is placed the residual liquid of a cleaning and is accommodated bottle, this cleans residual liquid and accommodates the opening that the port of bottle offers bottom this cleaning fluid reagent bottle via an input control electromagnetic valve and be connected, the efferent duct of this simulation digester communicates via one that control electromagnetic valve communicates with extraneous air or is isolated, the control end of this control electromagnetic valve that communicates, the control end of all input control electromagnetic valve, the control end of all output control electromagnetic valve, the control end of this air bath shaking table, the control end of this thermostatic water-circulator bath groove is connected with the control end of a Programmable Logic Controller, the control end of this direct peristaltic pump, all digestive juices are carried the control end of peristaltic pump, this cleaning fluid is carried the control end of peristaltic pump, the communication terminal of this Programmable Logic Controller is connected with the communication terminal of CPU.Wherein, the air bath shaking table is used for the simulation digester is implemented insulation and concussion function, with environment temperature in the simulated animal alimentary canal and gastral wriggling process (chyme mixed process), the air bath shaking table has been avoided between ambient temperature and simulation digester exchange heat taking place, thereby the fluctuation that ensures the digestion temperature that has in the dialysis tubing of simulation digester is no more than 0.4 ℃.The thermostatic water-circulator bath groove is used to heat damping fluid, makes damping fluid keep required constant temperature, and for preventing the thermal loss of damping fluid in transfer pipeline, the temperature that is provided with of thermostatic water-circulator bath groove should be higher 2～3 ℃ than the temperature in the air bath shaking table.CPU controls the flow and the flow velocity of the digestive juice in the digestive juice reagent bottle, with secretion of digestive juice and digestion process in the simulated animal body, CPU controlled flow, flow velocity and the time of circulating of the damping fluid in the damping fluid reagent bottle, with the gastral absorption process of simulated animal, CPU controlled flow, flow velocity, cycle index and the time of circulating of the cleaning fluid in the cleaning fluid reagent bottle, with the gastral evacuation procedure of simulated animal.Programmable Logic Controller is used to control the start and stop that communicate control electromagnetic valve, import control electromagnetic valve, export control electromagnetic valve, air bath shaking table and thermostatic water-circulator bath groove.

In actual design, preferably, the quantity of simulation digester can be two or more, and each is simulated to can be between the input pipe, efferent duct of digester and is connected in series, and correspondingly, each is simulated between the woven hose of digester to being connected in parallel.As Fig. 1 and Fig. 2, the quantity of the simulation digester 100 shown in the figure is four, and between the input pipe 114 of these four simulation digesters 100, efferent duct 113 for being connected in series, and between the woven hose 150 for being connected in parallel.This mode of being connected in series can guarantee that the electrochemical conditions in all simulation digesters is consistent, to reduce simulation error, improves simulation precision.

Extremely shown in Figure 5 as Fig. 3, simulation digester 100 comprises glass tube 110 and dialysis tubing (known technology) 120, this glass tube 110 is a hollow tube 111, the two ends of this body 111 respectively are provided with a ground (standard ground, as No. 19 standard grounds) 112,111 of ground 112 and bodys are seamless link, the side of this body 111 is provided with input pipe 114 and efferent duct 113, this dialysis tubing 120 is placed in this glass tube 110, the two ends of this dialysis tubing 120 are stretched out and are turned up from two grounds 112 of this glass tube 110 respectively, turn up dialysis tubing 120 ends that are exposed to ground 112 outsides are tied up by the rubber bar and are fixed on the ground 112, and two grounds 112 tying up behind dialysis tubing 120 ends are plugged with the rubber plug 140 that a rubber plug 130 and has woven hose 150 respectively.

In actual design, the setting that can be centrosymmetric with respect to the center of body 111 of input pipe 114 and efferent duct 113 is as Fig. 4 and Fig. 5.When reality is used, input pipe 114, efferent duct 113 lay respectively at below, top (placing vertically upward respectively vertically downward promptly), such placement design had both helped the circulation of damping fluid, and helping digesting the damping fluid that remains in the glass tube after the end again can be by thorough emptying.

In actual the manufacturing, the aperture of dialysis tubing 120 can be between 700 to 20000 dalton, dialysis tubing 120 is used for splendid attire feed sample and simulation digestive juice, the external diameter of the body 111 of glass tube 110 can be 30～45mm, the external diameter of this body 111 is than the big 10～35mm of external diameter of ground 112, the length of the body 111 of glass tube 110 can be 120～250mm, and glass tube 110 is used for feeding via input pipe 114 deionized water of damping fluid or cleaning usefulness.In addition, turning up the length of dialysis tubing 120 ends that are exposed to ground 112 outsides can be between 10 to 25mm.Woven hose 150 can be designed to L type structure, and as Fig. 3, the central part of turned welt rubber plug 140 is connected with a L plastic woven hose 150.This woven hose 150 is used for feeding the simulation digestive juice in dialysis tubing 120.This L type structural design of woven hose 150 can make the simulation digestive juice stablize, inject continuously in the dialysis tubing 120 via L type woven hose.

When reality is implemented, according to the factors such as digestion position of simulation digestion process, the quantity that the user can determine digestive juice reagent bottle and damping fluid reagent bottle with and the reagent type of interior placement.Listed several examples below, but the utility model is not limited to following these examples.

For example, the quantity of digestive juice reagent bottle and damping fluid reagent bottle can be one.This digestive juice reagent bottle and damping fluid reagent bottle be splendid attire peptic digest liquid, stomach damping fluid respectively, with the peptic digest process of simulation nonruminant; Perhaps this digestive juice reagent bottle and damping fluid reagent bottle are distinguished splendid attire small intestine digestive juice, small intestine damping fluid, with the small intestine digestion process of simulation nonruminant; Perhaps this digestive juice reagent bottle and damping fluid reagent bottle are distinguished splendid attire large intestine digestive juice, large intestine damping fluid, with the large intestine digestion process of simulation nonruminant.

Again for example, the quantity of digestive juice reagent bottle and damping fluid reagent bottle can be two, a digestive juice reagent bottle and a damping fluid reagent bottle are one group, wherein: digestive juice reagent bottle in a group and damping fluid reagent bottle be splendid attire peptic digest liquid, stomach damping fluid respectively, digestive juice reagent bottle in another group and damping fluid reagent bottle be splendid attire small intestine digestive juice, small intestine damping fluid respectively, with the stomach of independent simulation nonruminant, digestion process or the stomach of continuous analog nonruminant and the digestion process of small intestine of small intestine; Perhaps, digestive juice reagent bottle in one group and damping fluid reagent bottle be splendid attire small intestine digestive juice, small intestine damping fluid respectively, digestive juice reagent bottle in another group and damping fluid reagent bottle be splendid attire large intestine digestive juice, large intestine damping fluid respectively, with the small intestine of independent simulation nonruminant, digestion process or the small intestine of continuous analog nonruminant and the digestion process of large intestine of large intestine; Perhaps, digestive juice reagent bottle in one group and damping fluid reagent bottle be splendid attire peptic digest liquid, stomach damping fluid respectively, digestive juice reagent bottle in another group and damping fluid reagent bottle be splendid attire large intestine digestive juice, large intestine damping fluid respectively, with the stomach of independent simulation nonruminant, digestion process or the stomach of continuous analog nonruminant and the digestion process of large intestine of large intestine.

Again for example, the quantity of digestive juice reagent bottle and damping fluid reagent bottle can be three, a digestive juice reagent bottle and a damping fluid reagent bottle are one group, wherein: digestive juice reagent bottle in first group and damping fluid reagent bottle be splendid attire peptic digest liquid respectively, the stomach damping fluid, digestive juice reagent bottle in second group and damping fluid reagent bottle be splendid attire small intestine digestive juice respectively, the small intestine damping fluid, digestive juice reagent bottle in the 3rd group and damping fluid reagent bottle be splendid attire large intestine digestive juice respectively, the large intestine damping fluid is with the stomach of independent simulation nonruminant, small intestine, the stomach of the digestion process of large intestine or continuous analog nonruminant, the digestion process of small intestine and large intestine.

The utility model monogastric animal bionic digestive system illustrated in fig. 1 is the situation that disposes three digestive juice reagent bottles, three damping fluid reagent bottles, four simulation digesters, wherein: three digestive juice reagent bottles 510,509,508 are distinguished splendid attire peptic digest liquid, small intestine digestive juice, large intestine digestive juice, and three damping fluid reagent bottles 507,506,502 are distinguished splendid attire stomach damping fluids, small intestine damping fluid, large intestine damping fluid.As Fig. 1, shown in Figure 3, four simulation digesters 100 are placed on the fixed claw (not shown) in the air bath shaking table 200, between the input pipe 114 of four simulation digesters 100, the efferent duct 113 for being connected in series (efferent duct 113 of a simulation digester 100 is connected by silicone rubber tube with another 114 of input pipe of simulating digester 100), and between the woven hose 150 for being connected in parallel.When realistic simulation, the input pipe 114 of four simulation digesters 100 is placed vertically downward, and efferent duct 113 is placed (difference to that indicated in the drawings) vertically upward.The input pipe 114 of four simulation digesters 100, the input pipe 114 of a simulation digester 100 (i.e. the rightmost simulation digester of figure) of two the simulation digesters that are arranged in two ends that obtain after the series connection between the efferent duct 113 is connected with first input/output port of direct peristaltic pump 401, and another simulation digester (i.e. a leftmost simulation digester among the figure) efferent duct 113 of 100 with communicate control electromagnetic valve 601, input control electromagnetic valve 607,610,611 are connected with the input control electromagnetic valve 608 that links to each other with cleaning fluid reagent bottle 503 ports, three digestive juice reagent bottles 508,509,510 port is carried peristaltic pump 403 via digestive juice respectively, 404,405 with each the simulation digester 100 woven hose 150 be connected, the port that one waste liquid is accommodated bottle 501 is connected with second input/output port of this direct peristaltic pump 401 via an input control electromagnetic valve 602, be placed on three damping fluid reagent bottles 502 in the thermostatic water-circulator bath groove 300,506,507 port is respectively via output control electromagnetic valve 603,605,606 and be connected with second input/output port of this direct peristaltic pump 401, the port of cleaning fluid reagent bottle 503 is connected with second input/output port of this direct peristaltic pump 401 via an output control electromagnetic valve 604, the port of this cleaning fluid reagent bottle 503 carries peristaltic pump 402 to be connected with the port of a cleaning fluid storage bottle 505 via a cleaning fluid, the below of this cleaning fluid reagent bottle 503 is placed the residual liquid of a cleaning and is accommodated bottle 504, this cleans residual liquid and accommodates bottle 504 port and be connected with the opening that these cleaning fluid reagent bottle 503 bottoms are offered via an input control electromagnetic valve 609, the control electromagnetic valve that communicates 601 makes glass tube 110 communicate with extraneous air or is isolated, the control end of this control electromagnetic valve 601 that communicates, input control electromagnetic valve 602,607,608,609,610,611 control end, output control electromagnetic valve 603,604,605,606 control end, the control end of this air bath shaking table 200, the control end of this thermostatic water-circulator bath groove 300 is connected with the control end of a Programmable Logic Controller 700, the control end of this direct peristaltic pump 401, digestive juice is carried peristaltic pump 403,404,405 control end, this cleaning fluid carries the control end of peristaltic pump 402 by the RS485 communication interface, RS232 communication interface and being connected with the communication terminal (com port) of CPU 800, the communication terminal of this Programmable Logic Controller 700 is connected with the communication terminal (com port) of CPU800 by the RS232 communication interface.

Wherein, air bath shaking table 200 is used for simulation digester 100 is implemented insulation and concussion function, with environment temperature in the simulated animal alimentary canal and gastral wriggling process (chyme mixed process), air bath shaking table 200 has avoided ambient temperature and 100 of simulation digesters that exchange heat takes place, and has ensured that the fluctuation range of the digestion temperature that has in the dialysis tubing 120 of simulation digester 100 is no more than 0.4 ℃.Thermostatic water-circulator bath groove 300 is used to heat damping fluid, makes damping fluid keep required constant temperature, has prevented the thermal loss of damping fluid in transfer pipeline, and the temperature that is provided with of thermostatic water-circulator bath groove 300 should be higher 2～3 ℃ than the temperature in the air bath shaking table 200.The flow and the flow velocity of the digestive juice in 800 pairs of digestive juice reagent bottles of CPU are controlled, with secretion of digestive juice and digestion process in the simulated animal body, flow, flow velocity and the time of circulating of the damping fluid in 800 pairs of damping fluid reagent bottles of CPU are controlled, with the gastral absorption process of simulated animal, CPU800 controlled flow, flow velocity, cycle index and the time of circulating of the cleaning fluid in the cleaning fluid reagent bottle 503, with the gastral evacuation procedure of simulated animal.Programmable Logic Controller 700 is used to control the start and stop that communicate control electromagnetic valve 601, import control electromagnetic valve 602,607,608,609,610,611, export control electromagnetic valve 603,604,605,606, air bath shaking table 200, thermostatic water-circulator bath groove 300.

As shown in Figure 2, composition and the Fig. 1 of the utility model system shown in Fig. 2 are basic identical, the component no longer that Fig. 1 is identical with Fig. 2 here is described, difference between the two is: the quantity of the digestive juice reagent bottle among Fig. 2 is two, and the quantity of damping fluid reagent bottle is three, two digestive juice reagent bottles 509,508 are distinguished splendid attire small intestine digestive juices, large intestine digestive juice, and three damping fluid reagent bottles 507,506,502 are distinguished splendid attire stomach damping fluids, small intestine damping fluid, large intestine damping fluid.For the system shown in Fig. 2, when simulation peptic digest process, after in dialysis tubing 120, inserting the feed sample, peptic digest liquid person who happens to be on hand for an errand worker subsequently injects in the dialysis tubing 120, the peptic digest liquid that injects makes the feed sample be transferred to the middle part of dialysis tubing 120 fully, and then the simulation digester 100 that will put into feed sample and peptic digest liquid places on the fixed claw of air bath shaking table 200, then begins the simulation of peptic digest process.And for the system shown in Fig. 1, peptic digest liquid injects dialysis tubings 120 via woven hose 150 from digestive juice reagent bottle 510, rather than manually injects dialysis tubing 120.

Use of the present utility model mainly comprises preparation process, bionical digestion step, describes in detail below.

This preparation process specifically comprises the steps:

Step 1-1: open the thermostatic water-circulator bath groove, so that the damping fluid in all damping fluid reagent bottles of placing in it reaches design temperature separately;

Step 1-2: the feed sample packed into respectively simulate in the dialysis tubing of digester, each simulation digester is placed on the fixed claw in the air bath shaking table, carry peristaltic pump to be connected with digestive juice the woven hose of each simulation digester, input pipe with each simulation digester, be connected in series between the efferent duct, the input pipe of a simulation digester of two simulation digesters that are arranged in two ends that series connection obtains is connected with first input/output port of direct peristaltic pump, and the efferent duct that another simulates digester with communicate control electromagnetic valve, the input control electromagnetic valve that links to each other with the damping fluid reagent bottle is connected with the input control electromagnetic valve that links to each other with cleaning fluid reagent bottle port;

Step 1-3: open the air bath shaking table, the simulation digester on the fixed claw begins concussion.

This bionical digestion step specifically comprises the steps:

Step 2-1: the digestive juice to be measured of a scheduled volume carries peristaltic pump to inject dialysis tubing via woven hose from the digestive juice reagent bottle of this digestive juice to be measured of splendid attire by digestive juice corresponding with it, after this of one scheduled volume digestive juice to be measured injects dialysis tubing, close this digestive juice and carry peristaltic pump, the feed sample in the dialysis tubing carries out digestion reaction with this digestive juice to be measured;

Step 2-2: open the input control electromagnetic valve and the output control electromagnetic valve that link to each other with the damping fluid reagent bottle of splendid attire damping fluid to be measured, in a setting-up time, damping fluid to be measured in this damping fluid reagent bottle constantly circulates between the glass tube of this damping fluid reagent bottle and simulation digester by the running of direct peristaltic pump forward, so that the digestion product that produces behind digestive juice to be measured and the feed sample generation digestion reaction flows out in the damping fluid to be measured that dissolves in the glass tube by the molecular sieve on the dialysis tubing, when setting-up time finishes, close direct peristaltic pump and input control electromagnetic valve that links to each other with this damping fluid reagent bottle and output control electromagnetic valve;

Step 2-3: open the control electromagnetic valve that communicates, so that the glass tube of simulation digester communicates with air, unlatching is accommodated the input control electromagnetic valve that bottle links to each other with waste liquid, so that flowing into this waste liquid by the direct peristaltic pump antiport, this damping fluid to be measured in the glass tube of simulation digester accommodates in the bottle, after in the glass tube this damping fluid emptying to be measured, close direct peristaltic pump, the control electromagnetic valve that communicates with accommodate the input control electromagnetic valve that links to each other of bottle with waste liquid;

Step 2-4: open with cleaning residual liquid and accommodate the input control electromagnetic valve that bottle links to each other, the residual liquid in the cleaning fluid reagent bottle flows into automatically by draught head and cleans residual liquid and accommodate in the bottle, closes and cleans residual liquid and accommodate a bottle input control electromagnetic valve that links to each other;

Step 2-5: the cleaning fluid of a set amount carries peristaltic pump to inject the cleaning fluid reagent bottle from the cleaning fluid storage bottle by cleaning fluid, after the cleaning fluid of a set amount injects the cleaning fluid reagent bottle, closes cleaning fluid and carries peristaltic pump;

Step 2-6: open the input control electromagnetic valve and the output control electromagnetic valve that link to each other with cleaning fluid reagent bottle port, in a schedule time, cleaning fluid in the cleaning fluid reagent bottle constantly circulates between the glass tube of cleaning fluid reagent bottle and simulation digester by the running of direct peristaltic pump forward, so that the residual liquid in the glass tube is cleaned, the schedule time is closed direct peristaltic pump, the input control electromagnetic valve that links to each other with cleaning fluid reagent bottle port and output control electromagnetic valve when finishing;

Step 2-7: open the control electromagnetic valve that communicates, so that the glass tube of simulation digester communicates with air, unlatching is accommodated the input control electromagnetic valve that bottle links to each other with waste liquid, so that flowing into this waste liquid by the direct peristaltic pump antiport, the cleaning fluid in the glass tube of simulation digester accommodates in the bottle, after the cleaning fluid emptying in the glass tube, close direct peristaltic pump, the control electromagnetic valve that communicates with accommodate the input control electromagnetic valve that links to each other of bottle with waste liquid;

Step 2-8: open with the residual liquid of cleaning and accommodate the input control electromagnetic valve that bottle links to each other, residual liquid in the cleaning fluid reagent bottle flows into the residual liquid of cleaning automatically by draught head and accommodates in the bottle, after the residual liquid emptying in the cleaning fluid reagent bottle, close and clean residual liquid and accommodate the input control electromagnetic valve that links to each other of bottle;

Step 2-9: repeat above-mentioned steps 2-5 to step 2-8 several.

When practical application, above-mentioned digestive juice to be measured and damping fluid to be measured can be respectively peptic digest liquid, stomach damping fluid, and with the peptic digest process of simulation nonruminant, promptly above-mentioned bionical digestion step is the bionical digestion step of stomach; Perhaps above-mentioned digestive juice to be measured and damping fluid to be measured can be respectively small intestine digestive juice, small intestine damping fluid, and with the small intestine digestion process of simulation nonruminant, promptly above-mentioned bionical digestion step is the bionical digestion step of small intestine; Perhaps above-mentioned digestive juice to be measured and damping fluid to be measured can be respectively large intestine digestive juice, large intestine damping fluid, and with the large intestine digestion process of simulation nonruminant, promptly above-mentioned bionical digestion step is the bionical digestion step of large intestine.

Be example with the system shown in Fig. 1 below, illustrate and use detailed process of the present utility model.

At first, in system shown in Figure 1, can implement the simulation of peptic digest process, the simulation of small intestine digestion process, the simulation of large intestine digestion process separately, here repeat no more according to above-mentioned preparation process and bionical digestion step.

Secondly, in system shown in Figure 1, the user can carry out continuous analog to the digestion process of animal stomach and small intestine, and concrete steps are:

Preparation process: step 1-1: open thermostatic water-circulator bath groove 300, so that the damping fluid in the damping fluid reagent bottle of placing in it 502,506,507 reaches design temperature separately; Step 1-2: the feed sample is packed in the dialysis tubing 120 of respectively simulating digester 100, each simulation digester 100 is placed on the fixed claw in the air bath shaking table 200, each simulates the input pipe 114 of digester 100, be connected in series between the efferent duct 113 (efferent duct 113 of a simulation digester 100 is connected by silicone rubber tube with 114 of the input pipes of another simulation digester 100), and be connected in parallel between the woven hose 150, each is simulated the input pipe 114 of digester 100 and places vertically downward, and efferent duct 113 is placed vertically upward, the input pipe 114 of simulation digester 100, the input pipe 114 of a simulation digester 100 of two the simulation digesters that are arranged in two ends that obtain after the series connection between the efferent duct 113 is connected with first input/output port of direct peristaltic pump 401, and another the simulation digester 100 efferent duct 113 with communicate control electromagnetic valve 601, input control electromagnetic valve 607,610,611 are connected digestive juice reagent bottle 508 with the input control electromagnetic valve 608 that links to each other with cleaning fluid reagent bottle 503 ports, 509,510 port is carried peristaltic pump 403 via digestive juice respectively, 404,405 with each the simulation digester 100 woven hose 150 be connected; Step 1-3: open air bath shaking table 200, the simulation digester 100 on the fixed claw begins concussion.

The bionical digestion step of stomach: step 2-1 ': the peptic digest liquid of a scheduled volume carries peristaltic pump 405 to inject dialysis tubing 120 via woven hose 150 from digestive juice reagent bottle 510 by digestive juice, after this peptic digest liquid injects dialysis tubing 120, close this digestive juice and carry peristaltic pump 405, the feed sample in the dialysis tubing 120 and this peptic digest liquid carry out digestion reaction; Step 2-2 ': open the input control electromagnetic valve 611 and the output control electromagnetic valve 606 that link to each other with the damping fluid reagent bottle 507 of splendid attire stomach damping fluid, in a setting-up time (0 to 48 hour), 110 of glass tubes at this damping fluid reagent bottle 507 and simulation digester 100 constantly circulate (damping fluid reagent bottle 507 → direct peristaltic pump 401 → glass tube 110 → damping fluid reagent bottle 507) to this stomach damping fluid by the running of direct peristaltic pump 401 forwards, (digestion product of generation is taken away by the stomach damping fluid in the stomach damping fluid that dissolves in the glass tube 110 so that the digestion product that produces behind peptic digest liquid and the feed sample generation digestion reaction flows out by the molecular sieve on the dialysis tubing 120, and can not get back in the dialysis tubing 120, so that the digestion product that produces can not influence other reactions of carrying out subsequently in the dialysis tubing 120, only stayed not digestion product in the dialysis tubing 120), when setting-up time finishes, close direct peristaltic pump 401 and input control electromagnetic valve 611 that links to each other with this damping fluid reagent bottle 507 and output control electromagnetic valve 606; Step 2-3 ': open the control electromagnetic valve 601 that communicates, so that the glass tube 110 of simulation digester 100 communicates with air, unlatching is accommodated the bottle 501 input control electromagnetic valve 602 that link to each other with waste liquid, so that flowing into this waste liquid by direct peristaltic pump 401 antiports, this stomach damping fluid in the glass tube 110 of simulation digester 100 accommodates (glass tube 110 → direct peristaltic pump 401 → waste liquid is accommodated bottle 501) in the bottle 501, after this stomach damping fluid emptying in the glass tube 110, close direct peristaltic pump 401, the control electromagnetic valve that communicates 601 with waste liquid accommodate the bottle 501 input control electromagnetic valve 602 that link to each other; Step 2-4 ': open with the residual liquid of cleaning and accommodate bottle 504 input control electromagnetic valve 609 that link to each other, residual liquid in the cleaning fluid reagent bottle 503 flows into automatically by draught head and cleans residual liquid and accommodate in the bottle 504, closes and cleans residual liquid and accommodate bottle 504 input control electromagnetic valve 609 that link to each other; Step 2-5 ': the cleaning fluid of a set amount carries peristaltic pump 402 to inject cleaning fluid reagent bottle 503 from cleaning fluid storage bottle 505 by cleaning fluid, after cleaning fluid injects cleaning fluid reagent bottle 503, closes cleaning fluid and carries peristaltic pump 402; Step 2-6 ': open the input control electromagnetic valve 608 and the output control electromagnetic valve 604 that link to each other with cleaning fluid reagent bottle 503 ports, in a schedule time (0 to 2 hour), 110 of glass tubes at cleaning fluid reagent bottle 503 and simulation digester 100 constantly circulate (cleaning fluid reagent bottle 503 → direct peristaltic pump 401 → glass tube 110 → cleaning fluid reagent bottle 503) to cleaning fluid in the cleaning fluid reagent bottle 503 (deionized water) by the running of direct peristaltic pump 401 forwards, so that the residual liquid (being dissolved with the stomach damping fluid of digestion product) in the glass tube 110 is cleaned, the schedule time is closed direct peristaltic pump 401 when finishing, input control electromagnetic valve 608 that links to each other with cleaning fluid reagent bottle 503 ports and output control electromagnetic valve 604; Step 2-7 ': open the control electromagnetic valve 601 that communicates, so that the glass tube 110 of simulation digester 100 communicates with air, unlatching is accommodated the bottle 501 input control electromagnetic valve 602 that link to each other with waste liquid, so that flowing into this waste liquid by direct peristaltic pump 401 antiports, the cleaning fluid in the glass tube 110 of simulation digester 100 accommodates (glass tube 110 → direct peristaltic pump 401 → waste liquid is accommodated bottle 501) in the bottle 501, after the cleaning fluid emptying in the glass tube 110, close direct peristaltic pump 401, the control electromagnetic valve that communicates 601 with waste liquid accommodate the bottle 501 input control electromagnetic valve 602 that link to each other; Step 2-8 ': open with the residual liquid of cleaning and accommodate bottle 504 input control electromagnetic valve 609 that link to each other, residual liquid in the cleaning fluid reagent bottle 503 flows into the residual liquid of cleaning automatically by draught head and accommodates in the bottle 504, after the residual liquid emptying in the cleaning fluid reagent bottle 503, close with clean residual liquid accommodate the bottle 504 input control electromagnetic valve 609 that link to each other; Step 2-9 ': repeat above-mentioned steps 2-5 ' to step 2-8 ' for several times.At this moment, the simulation digestion process of stomach is finished.

The bionical digestion step of small intestine: step 3-1: open the input control electromagnetic valve 610 and the output control electromagnetic valve 605 that link to each other with the damping fluid reagent bottle 506 of splendid attire small intestine damping fluid, be provided with in the time (0 to 2 hour) one, 110 of glass tubes at this damping fluid reagent bottle 506 and simulation digester 100 constantly circulate (damping fluid reagent bottle 506 → direct peristaltic pump 401 → glass tube 110 → damping fluid reagent bottle 506) to small intestine damping fluid in this damping fluid reagent bottle 506 by the running of direct peristaltic pump 401 forwards,, the ion concentration in glass tube 110 and the dialysis tubing 120 creates the small intestine digestive environments so that equating, the time that is provided with is when finishing, the small intestine digestive juice of one scheduled volume carries peristaltic pump 404 to inject dialysis tubing 120 via woven hose 150 from the digestive juice reagent bottle 509 of this small intestine digestive juice of splendid attire by digestive juice, after this small intestine digestive juice injects dialysis tubing 120, close this digestive juice and carry peristaltic pump 404, residual not digestion product and this small intestine digestive juice in the dialysis tubing 120 carry out digestion reaction, when injecting the small intestine digestive juice, 110 of glass tubes at this damping fluid reagent bottle 506 and simulation digester 100 constantly circulate by the running of direct peristaltic pump 401 forwards in this small intestine damping fluid continuation, (the new digestion product of generation is taken away by the small intestine damping fluid in the small intestine damping fluid that dissolves in the glass tube 110 so that the new digestion product that produces behind the residual not digestion product generation digestion reaction in small intestine digestive juice and the dialysis tubing 120 flows out by the molecular sieve on the dialysis tubing 120, and can not get back in the dialysis tubing 120, so that the new digestion product that produces can not influence other reactions of carrying out subsequently in the dialysis tubing 120, only stayed in the dialysis tubing 120 through the not digestion product behind the small intestine digestion reaction), the small intestine damping fluid is closed direct peristaltic pump 401 after continuing to circulate a setting-up time (0 to 48 hour), input control electromagnetic valve 610 that links to each other with this damping fluid reagent bottle 506 and output control electromagnetic valve 605; Step 3-2: open the control electromagnetic valve 601 that communicates, so that the glass tube 110 of simulation digester 100 communicates with air, unlatching is accommodated the bottle 501 input control electromagnetic valve 602 that link to each other with waste liquid, so that flowing into this waste liquid by direct peristaltic pump 401 antiports, this small intestine damping fluid in the glass tube 110 of simulation digester 100 accommodates (glass tube 110 → direct peristaltic pump 401 → waste liquid is accommodated bottle 501) in the bottle 501, after this small intestine damping fluid emptying in the glass tube 110, close direct peristaltic pump 401, the control electromagnetic valve that communicates 601 with waste liquid accommodate the bottle 501 input control electromagnetic valve 602 that link to each other; Step 3-3: open with the residual liquid of cleaning and accommodate bottle 504 input control electromagnetic valve 609 that link to each other, residual liquid in the cleaning fluid reagent bottle 503 flows into automatically by draught head and cleans residual liquid and accommodate in the bottle 504, closes and cleans residual liquid and accommodate bottle 504 input control electromagnetic valve 609 that link to each other; Step 3-4: the cleaning fluid of a set amount carries peristaltic pump 402 to inject cleaning fluid reagent bottle 503 from cleaning fluid storage bottle 505 by cleaning fluid, after cleaning fluid injects cleaning fluid reagent bottle 503, closes cleaning fluid and carries peristaltic pump 402; Step 3-5: open the input control electromagnetic valve 608 and the output control electromagnetic valve 604 that link to each other with cleaning fluid reagent bottle 503 ports, in a schedule time (0 to 2 hour), 110 of glass tubes at cleaning fluid reagent bottle 503 and simulation digester 100 constantly circulate (cleaning fluid reagent bottle 503 → direct peristaltic pump 401 → glass tube 110 → cleaning fluid reagent bottle 503) to cleaning fluid in the cleaning fluid reagent bottle 503 (deionized water) by the running of direct peristaltic pump 401 forwards, so that the residual liquid (being dissolved with the small intestine damping fluid of new digestion product) in the glass tube 110 is cleaned, the schedule time is closed direct peristaltic pump 401 when finishing, input control electromagnetic valve 608 that links to each other with cleaning fluid reagent bottle 503 ports and output control electromagnetic valve 604; Step 3-6: open the control electromagnetic valve 601 that communicates, so that the glass tube 110 of simulation digester 100 communicates with air, unlatching is accommodated the bottle 501 input control electromagnetic valve 602 that link to each other with waste liquid, so that flowing into waste liquid by direct peristaltic pump 401 antiports, the cleaning fluid in the glass tube 110 of simulation digester 100 accommodates (glass tube 110 → direct peristaltic pump 401 → waste liquid is accommodated bottle 501) in the bottle 501, after the cleaning fluid emptying in the glass tube 110, close direct peristaltic pump 401, the control electromagnetic valve that communicates 601 with waste liquid accommodate the bottle 501 input control electromagnetic valve 602 that link to each other; Step 3-7: open with the residual liquid of cleaning and accommodate bottle 504 input control electromagnetic valve 609 that link to each other, residual liquid in the cleaning fluid reagent bottle 503 flows into the residual liquid of cleaning automatically by draught head and accommodates in the bottle 504, after the residual liquid emptying in the cleaning fluid reagent bottle 503, close with clean residual liquid accommodate the bottle 504 input control electromagnetic valve 609 that link to each other; Step 3-8: repeat above-mentioned steps 3-4 to step 3-7 several.At this moment, the simulation digestion process of small intestine is finished.

Once more, in system shown in Figure 1, the user also can carry out continuous analog to the digestion process of animal stomach, small intestine and large intestine, promptly implements the following bionical digestion step of large intestine after above-mentioned preparation process, the bionical digestion step of stomach, the bionical digestion step of small intestine.

The bionical digestion step of large intestine is specially: step 4-1: open the input control electromagnetic valve 607 and the output control electromagnetic valve 603 that link to each other with the damping fluid reagent bottle 502 of splendid attire large intestine damping fluid, be provided with in the time (0 to 2 hour) one, 110 of glass tubes at this damping fluid reagent bottle 502 and simulation digester 100 constantly circulate (damping fluid reagent bottle 502 → direct peristaltic pump 401 → glass tube 110 → damping fluid reagent bottle 502) to large intestine damping fluid in this damping fluid reagent bottle 502 by the running of direct peristaltic pump 401 forwards,, the ion concentration in glass tube 110 and the dialysis tubing 120 creates the large intestine digestive environments so that equating, the time that is provided with is when finishing, the large intestine digestive juice of one scheduled volume carries peristaltic pump 403 to inject dialysis tubing 120 via woven hose 150 from the digestive juice reagent bottle 508 of this large intestine digestive juice of splendid attire by digestive juice, after this large intestine digestive juice injects dialysis tubing 120, close this digestive juice and carry peristaltic pump 403, residual not digestion product and this large intestine digestive juice in the dialysis tubing 120 carry out digestion reaction, when injecting the large intestine digestive juice, 110 of glass tubes at this damping fluid reagent bottle 502 and simulation digester 100 constantly circulate by the running of direct peristaltic pump 401 forwards in this large intestine damping fluid continuation, (the new digestion product of generation is taken away by the large intestine damping fluid in the large intestine damping fluid that dissolves in the glass tube 110 so that the new digestion product that produces behind the residual not digestion product generation digestion reaction in large intestine digestive juice and the dialysis tubing 120 flows out by the molecular sieve on the dialysis tubing 120, and can not get back in the dialysis tubing 120, so that the new digestion product that produces can not influence other reactions of carrying out subsequently in the dialysis tubing 120, only stayed in the dialysis tubing 120 through the not digestion product behind the large intestine digestion reaction), the large intestine damping fluid is closed direct peristaltic pump 401 after continuing to circulate a setting-up time (0 to 48 hour), input control electromagnetic valve 607 that links to each other with this damping fluid reagent bottle 502 and output control electromagnetic valve 603; Step 4-2: open the control electromagnetic valve 601 that communicates, so that the glass tube 110 of simulation digester 100 communicates with air, unlatching is accommodated the bottle 501 input control electromagnetic valve 602 that link to each other with waste liquid, so that flowing into this waste liquid by direct peristaltic pump 401 antiports, this large intestine damping fluid in the glass tube 110 of simulation digester 100 accommodates (glass tube 110 → direct peristaltic pump 401 → waste liquid is accommodated bottle 501) in the bottle 501, after this large intestine damping fluid emptying in the glass tube 110, close direct peristaltic pump 401, the control electromagnetic valve that communicates 601 with waste liquid accommodate the bottle 501 input control electromagnetic valve 602 that link to each other; Step 4-3: open with the residual liquid of cleaning and accommodate bottle 504 input control electromagnetic valve 609 that link to each other, residual liquid in the cleaning fluid reagent bottle 503 flows into automatically by draught head and cleans residual liquid and accommodate in the bottle 504, closes and cleans residual liquid and accommodate bottle 504 input control electromagnetic valve 609 that link to each other; Step 4-4: the cleaning fluid of a set amount carries peristaltic pump 402 to inject cleaning fluid reagent bottle 503 from cleaning fluid storage bottle 505 by cleaning fluid, after cleaning fluid injects cleaning fluid reagent bottle 503, closes cleaning fluid and carries peristaltic pump 402; Step 4-5: open the input control electromagnetic valve 608 and the output control electromagnetic valve 604 that link to each other with cleaning fluid reagent bottle 503 ports, in a schedule time (0 to 2 hour), 110 of glass tubes at cleaning fluid reagent bottle 503 and simulation digester 100 constantly circulate (cleaning fluid reagent bottle 503 → direct peristaltic pump 401 → glass tube 110 → cleaning fluid reagent bottle 503) to cleaning fluid in the cleaning fluid reagent bottle 503 (deionized water) by the running of direct peristaltic pump 401 forwards, so that the residual liquid (being dissolved with the large intestine damping fluid of new digestion product) in the glass tube 110 is cleaned, the schedule time is closed direct peristaltic pump 401 when finishing, input control electromagnetic valve 608 that links to each other with cleaning fluid reagent bottle 503 ports and output control electromagnetic valve 604; Step 4-6: open the control electromagnetic valve 601 that communicates, so that the glass tube 110 of simulation digester 100 communicates with air, unlatching is accommodated the bottle 501 input control electromagnetic valve 602 that link to each other with waste liquid, so that flowing into waste liquid by direct peristaltic pump 401 antiports, the cleaning fluid in the glass tube 110 of simulation digester 100 accommodates (glass tube 110 → direct peristaltic pump 401 → waste liquid is accommodated bottle 501) in the bottle 501, after the cleaning fluid emptying in the glass tube 110, close direct peristaltic pump 401, the control electromagnetic valve that communicates 601 with waste liquid accommodate the bottle 501 input control electromagnetic valve 602 that link to each other; Step 4-7: open with the residual liquid of cleaning and accommodate bottle 504 input control electromagnetic valve 609 that link to each other, residual liquid in the cleaning fluid reagent bottle 503 flows into the residual liquid of cleaning automatically by draught head and accommodates in the bottle 504, after the residual liquid emptying in the cleaning fluid reagent bottle 503, close with clean residual liquid accommodate the bottle 504 input control electromagnetic valve 609 that link to each other; Step 4-8: repeat above-mentioned steps 4-4 to step 4-7 several.At this moment, the simulation digestion process of large intestine is finished.

The process of the utility model system that the use of the utility model system shown in Fig. 2 and above-mentioned use are shown in Figure 1 is roughly the same, difference between the two is: because the quantity of the digestive juice reagent bottle among Fig. 2 is two, the quantity of damping fluid reagent bottle is three, two digestive juice reagent bottles 509,508 difference splendid attire small intestine digestive juices, the large intestine digestive juice, three damping fluid reagent bottles 507,506,502 difference splendid attire stomach damping fluids, the small intestine damping fluid, the large intestine damping fluid, therefore, when using the system simulation peptic digest process shown in Fig. 2, after in dialysis tubing 120, inserting the feed sample, peptic digest liquid is to inject manually subsequently in the dialysis tubing 1 20, the peptic digest liquid that injects is used to make the feed sample to be transferred to the middle part of dialysis tubing 120 fully, and then the simulation digester 100 that will put into feed sample and peptic digest liquid places on the fixed claw of air bath shaking table 200, then begin the simulation of peptic digest process, rather than the digester 100 of will simulating earlier described above places on the fixed claw, and then peptic digest liquid is injected dialysis tubings 120 via woven hose 150 from digestive juice reagent bottle 510.

It should be noted that the usable range of the utility model system is not limited to the digestion process of the digestion process of above-mentioned independent simulation stomach, small intestine, large intestine, continuous analog stomach, small intestine, and the digestion process of continuous analog stomach, small intestine and large intestine.

The utlity model has following advantage:

The above is preferred embodiment of the present utility model and the know-why used thereof; for a person skilled in the art; under the situation that does not deviate from spirit and scope of the present utility model; any based on conspicuous changes such as the equivalent transformation on the technical solutions of the utility model basis, simple replacements, all belong to protection domain of the present utility model.

Claims

1, a kind of monogastric animal bionic digestive system is characterized in that: it comprises that simulation digester, digestive juice reagent bottle, damping fluid reagent bottle, waste liquid accommodate bottle, cleaning fluid storage bottle, cleaning fluid reagent bottle and clean residual liquid and accommodate bottle, wherein:

At least one simulation digester is placed on the interior fixed claw of an air bath shaking table, the port of at least one digestive juice reagent bottle is via carrying peristaltic pump to be connected with the woven hose of this simulation digester with himself corresponding digestive juice, first input/output port of one direct peristaltic pump is connected with the input pipe of this simulation digester, the port that one waste liquid is accommodated bottle is connected with second input/output port of this direct peristaltic pump via an input control electromagnetic valve, the port that is placed at least one damping fluid reagent bottle in the thermostatic water-circulator bath groove is via being connected with second input/output port of this direct peristaltic pump with himself corresponding output control electromagnetic valve, the port of this at least one damping fluid reagent bottle via with himself corresponding one the input control electromagnetic valve be connected with the efferent duct of this simulation digester, the port of one cleaning fluid reagent bottle is connected with second input/output port of this direct peristaltic pump via an output control electromagnetic valve, the port of this cleaning fluid reagent bottle is respectively via an input control electromagnetic valve, one cleaning fluid is carried the efferent duct of peristaltic pump and this simulation digester, the port of one cleaning fluid storage bottle connects, the below of this cleaning fluid reagent bottle is placed the residual liquid of a cleaning and is accommodated bottle, this cleans residual liquid and accommodates the opening that the port of bottle offers bottom this cleaning fluid reagent bottle via an input control electromagnetic valve and be connected, the efferent duct of this simulation digester communicates via one that control electromagnetic valve communicates with extraneous air or is isolated

The control end of the control end of the control end of this control electromagnetic valve that communicates, all input control electromagnetic valve, all output control electromagnetic valve, the control end of this air bath shaking table, the control end of this thermostatic water-circulator bath groove are connected with the control end of a Programmable Logic Controller, and the control end of this direct peristaltic pump, all digestive juices carry control end, this cleaning fluid of peristaltic pump to carry the control end of peristaltic pump, the communication terminal of this Programmable Logic Controller to be connected with the communication terminal of CPU.

2, monogastric animal bionic digestive system according to claim 1 is characterized in that:

The quantity of described digestive juice reagent bottle and described damping fluid reagent bottle is one, described digestive juice reagent bottle and described damping fluid reagent bottle be splendid attire peptic digest liquid, stomach damping fluid respectively, perhaps described digestive juice reagent bottle and described damping fluid reagent bottle be splendid attire small intestine digestive juice, small intestine damping fluid respectively, and perhaps described digestive juice reagent bottle and described damping fluid reagent bottle be splendid attire large intestine digestive juice, large intestine damping fluid respectively.

3, monogastric animal bionic digestive system according to claim 1 is characterized in that:

The quantity of described digestive juice reagent bottle and described damping fluid reagent bottle is two, and a described digestive juice reagent bottle and a described damping fluid reagent bottle are one group, wherein:

Described digestive juice reagent bottle in one group and described damping fluid reagent bottle be splendid attire peptic digest liquid, stomach damping fluid respectively, and described digestive juice reagent bottle in another group and described damping fluid reagent bottle be splendid attire small intestine digestive juice, small intestine damping fluid respectively; Perhaps, described digestive juice reagent bottle in a group and described damping fluid reagent bottle be splendid attire small intestine digestive juice, small intestine damping fluid respectively, and described digestive juice reagent bottle in another group and described damping fluid reagent bottle be splendid attire large intestine digestive juice, large intestine damping fluid respectively; Perhaps, described digestive juice reagent bottle in a group and described damping fluid reagent bottle be splendid attire peptic digest liquid, stomach damping fluid respectively, and described digestive juice reagent bottle in another group and described damping fluid reagent bottle be splendid attire large intestine digestive juice, large intestine damping fluid respectively.

4, monogastric animal bionic digestive system according to claim 1 is characterized in that:

The quantity of described digestive juice reagent bottle and described damping fluid reagent bottle is three, and a described digestive juice reagent bottle and a described damping fluid reagent bottle are one group, wherein:

Described digestive juice reagent bottle in first group and described damping fluid reagent bottle be splendid attire peptic digest liquid, stomach damping fluid respectively, described digestive juice reagent bottle in second group and described damping fluid reagent bottle be splendid attire small intestine digestive juice, small intestine damping fluid respectively, and described digestive juice reagent bottle in the 3rd group and described damping fluid reagent bottle be splendid attire large intestine digestive juice, large intestine damping fluid respectively.

5, monogastric animal bionic digestive system according to claim 1 is characterized in that:

The quantity of described digestive juice reagent bottle is two, and the quantity of described damping fluid reagent bottle is three, wherein:

Two described digestive juice reagent bottles are distinguished splendid attire small intestine digestive juice, large intestine digestive juice, and three described damping fluid reagent bottles are distinguished splendid attire stomach damping fluid, small intestine damping fluid, large intestine damping fluid.

6, according to each described monogastric animal bionic digestive system in the claim 1 to 5, it is characterized in that:

The quantity of described simulation digester is two or more, between the input pipe of each described simulation digester, the efferent duct for being connected in series, between the woven hose of each described simulation digester for being connected in parallel;

Described simulation digester comprises glass tube and dialysis tubing, this glass tube is a hollow tube, the two ends of this body respectively are provided with a ground, the side of this body is provided with described input pipe and described efferent duct, this dialysis tubing is placed in this glass tube, the two ends of this dialysis tubing are stretched out and are turned up from two grounds of this glass tube respectively, the turn up dialysis tubing end that is exposed to the ground outside is tied up by the rubber bar and is fixed on the ground, and two grounds tying up behind the dialysis tubing end are plugged with the rubber plug that a rubber plug and has described woven hose respectively.