CN103675205B - A kind of device simulating gastric digestion and using method - Google Patents
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Abstract
A kind of device simulating gastric digestion and using method, including stomach reactor, agitator, water-bath control system, computer supervisory control system, gastric emptying pump etc..Gastric juice and determinand solution or dispersion liquid is added to stomach reactor, the wriggling of agitator simulation stomach, water-bath control system controls the temperature in reactor, and by computer prison system monitoring stomach reactor internal temperature, pH and digestion time, gastric emptying is simulated by gastric emptying constant flow pump, by postdigestive product collection, go depepsinized activity；Simulation digestion process in triplicate, detects the digestion situation of test substance.The present invention simulates the digestion process that stomach is concrete, detailed first, various activity or nutrient substance can be entered the digestion situation observation after human body, explore the gastric digestion situation after various activity or nutrient substance entrance human body, simulated environment is carried out according to the physiological environment of human body, strong with the gastric digestion stickiness of people, in terms of controlling simulated environment, there is stronger accuracy.
Description
Technical field
The present invention relates to a kind of device simulating gastric digestion and using method.
Background technology
World's authoritative journal represents, study the effect in body of food or active substance and mechanism of action is most important, because illustrating of internal mechanism of action can be to utilize food or active substance to provide Research foundation substantially, it is made to play physiological function in human body substantially, simultaneously to avoiding its possible adverse side effect of generation to have directive significance.The physiological function of food or the actual performance of active substance is heavily dependent on what its product after human consumption is.For the consideration of the moral ethics for human body In vivo study, in-vitro simulated human consumption just becomes the key problem in technology of study on mechanism in food or active substance body.Dynamic analog is carried out by controlling the enzyme etc. in the key point such as temperature of human consumption's process, Digestive system.Simultaneously, the digestive system of simulation has preferable degree of accuracy compared with the digestive system of human body, favorable reproducibility, it is easily controlled, the digestion product at each position can be collected at any time, when experiment relates to the situation of toxic chemical substance, will not be retrained by morals, be offered convenience to the research of the active substance mechanism of action.Therefore, in vitro digestion is most important.
Stomach is the place that material enters first dominant digestion that human body runs into after the digestion in oral cavity.The energy that human motion needs comes from digestive organs absorption mesotrophic to food, and food has good digestion process in stomach, can be beneficial to overall Alimentary work.In stomach for digest main generation effect be gastric acid and pepsin.Stomach, by stirring food of wriggling, makes food and gastric juice be sufficiently mixed and carry out protopepsia.Process relevant with digestion in stomach is the secretion of gastric acid, pepsic effect and the process of gastric emptying in gastric juice.Different food or the most digested situation of active nutritional material are not quite similar, it is the most meaningful that this makes to study gastric digestion, because research understands that different material digestible energy under one's belt digests research further and provides data for this material, simultaneously for understanding and the digestion method important in inhibiting of exploration different material.The Patents relating to gastric digestion simulation in recent years has: " intelligent biology body pipe intestinal digesting system simulation controls device " (application number: 201220211927.6)；" Simulative digestive device for gastrointestinal tract of animals " (application number: 200920105937.X)；" Bionic digestive system for monogastric animals and method based on the digestion of this system simulation nonruminant " (application number: 200910078147.1)；" broiler external digestion simulating device (200810100818.5)；" a kind of analog device of alimentary system " (application number: 200710078118.6).These patent reports enrich device and the using method being applicable to gastric digestion greatly, but up to the present, these patents do not have detailed simulation and explanation for the preparation of gastric juice, the evacuation procedure of stomach, some is only relevant with animal for these patents simultaneously, little with the relatedness of people.
Summary of the invention
It is an object of the invention to provide a kind of device simulating gastric digestion and using method.Gastric digestion situation after this device and using method can enter human body to various activity or nutrient substance is observed, and helps the gastric digestion situation after exploring various activity or nutrient substance entrance human body.
The present invention is achieved by the following technical solutions.
The device of simulation gastric digestion of the present invention includes that determinand holds ware (1), hydrochloric acid holds ware (2), determinand conveying constant flow pump (3), hydrochloric acid carry constant flow pump (4), stomach reactor (5), magnetic stirring apparatus (6), temperature and pH monitoring system (7), gastric emptying constant flow pump (8), determinand are collected and held ware (9), water-bath control system (10), pH meter (11), thermometer (12).The inside of stomach reactor (5) is reative cell, and outer layer is water-bath layer.Determinand is held ware (1) and is connected with determinand conveying constant flow pump (3) by flexible pipe, and the other end of determinand conveying constant flow pump (3) stretches into the inside of stomach reactor (5) by flexible pipe；Hydrochloric acid is held ware (2) and is connected with hydrochloric acid conveying constant flow pump (4) by flexible pipe, and the other end of hydrochloric acid conveying constant flow pump (4) stretches into the inside of stomach reactor (5) by flexible pipe；Determinand collection is held ware (9) and is connected with gastric emptying constant flow pump (8) by flexible pipe, and the other end of gastric emptying constant flow pump (8) is exported by the hose connection inner bottom to stomach reactor (5)；Stomach reactor (5) is placed on magnetic stirring apparatus (6)；Temperature and pH monitoring system (7) connect pH meter (11) and thermometer (12) respectively, and pH meter (11) and thermometer (12) are suspended in the inside of stomach reactor (5)；Water-bath control system (10) is connected by flexible pipe 2 water-baths upper and lower with the outer layer of stomach reactor (5) outlet.
The using method of the device of simulation gastric digestion of the present invention is: add the gastric juice of q.s to the inside of stomach reactor (5), determinand holds the determinand solution in ware (1) or dispersion liquid by determinand conveying constant flow pump (3) with≤5
Ml/min flow velocity adds the inside of stomach reactor (5) and carries out stomach simulation digestion.Open magnetic stirring apparatus (6), wriggling with the constant mixing speed simulation stomach of 200-300 rpm/min, the temperature in reactor is controlled by water-bath control system (10), and the internal temperature of the thermometer (12) monitoring stomach reactor (5) connected by temperature and pH monitoring system (7), keep constant 37 DEG C.Simultaneously, by the internal pH of the pH meter (11) monitoring stomach reactor (5) that temperature and pH monitoring system (7) connect, once pH is too high, then carry constant flow pump (4) with hydrochloric acid and regulate and control with the constant speed addition 0.1-0.2 M hydrochloric acid of 1-2 ml/min, remain that pH is 2-3.The time of stomach simulation digestion is 5-6 h, by gastric emptying constant flow pump (8) with the emptying of 4-6 ml/min flow velocity simulation stomach, is collected in determinand by postdigestive product collection and holds in ware (9), obtain peptic digestion product.Afterwards, by the digestion product collected in boiling water bath a few minutes to go depepsinized activity.Simulation digestion process in triplicate, detects the digestion situation of test substance with liquid phase method.
The craft feature of the present invention is that this device and using method specifically digest have detailed description for simulation stomach first, specification for simulation gastric digestion is furnished with detailed description first, and digestion process corresponding for stomach has been also carried out detailed simulation explanation first.
The solution have the advantages that: the gastric digestion situation after this device and using method can enter human body to various activity or nutrient substance is observed, help the gastric digestion situation after exploring various activity or nutrient substance entrance human body.
The emptying simulation that the present invention carries stomach function regulating for gastric juice is all described in detail, and simulated environment is carried out according to the physiological environment of human body, and the gastric digestion stickiness of people is strong.The present invention uses computer to automatically control the parameters such as the pH in stomach analogue reactor simultaneously, has stronger accuracy than prior art in terms of controlling simulated environment.
Accompanying drawing explanation
Fig. 1 is the device schematic diagram of simulation gastric digestion.Wherein, 1 ware is held for determinand；2 hold ware for hydrochloric acid；3 carry constant flow pump for determinand；4 carry constant flow pump for hydrochloric acid；5 is stomach reactor；6 is magnetic stirring apparatus；7 is temperature and pH monitoring system；8 is gastric emptying constant flow pump；9 hold ware for determinand collection；10 is water-bath control system；11 is pH meter；12 is thermometer.
Detailed description of the invention
The present invention will be described further by following example.
According to above-mentioned concrete operation step, at the Imitating gastric digestion that imposes a condition.
Embodiment 1.
The stomach simulation digestion of vegetable polysaccharides.
(1) preparation of gastric juice.
Gastric juice is to weigh 37.5 mg gastric lipases, and 35.4 mg pepsin join the (preparation of stomach electrolyte solution: weigh 3.1 g in 150 g stomach electrolyte
NaCl, 1 g KCl, 0.15 g CaCl2, 0.6 g NaHCO3, with deionized water constant volume in the volumetric flask of 1 L, with the HCl of 0.1 M, electrolyte solution pH is adjusted to 3), it is subsequently adding 1.5 mL CH3COONa(1 M, pH 5).Magnetic agitation 10 min under room temperature, is adjusted to 2 with the HCl of 0.1 M by pH value of solution, is placed in refrigerator standby.
(2) simulation gastric digestion.
First, the vegetable polysaccharides solution (5mg/ml) first passing through oral cavity simulation digestion the most in advance is placed in ware.
Then, gastric digestion uses controlled trial method to be grouped as follows.
Experimental group 1: add gastric acid in analog, gastric juice and vegetable polysaccharides solution carry out stomach simulation digestion.
Experimental group 2: add gastric acid in analog, gastric juice and water (with the vegetable polysaccharides solution same volume in experimental group 1) carry out stomach simulation digestion, and this experimental group is for removing material own in gastric acid and gastric juice for analyzing the interference of vegetable polysaccharides gastric digestion situation.
Experimental group 3: adding vegetable polysaccharides solution in analog and water (with the gastric acid in experimental group 1 and gastric juice same volume) carries out stomach simulation digestion, this experimental group is for removing the impact that vegetable polysaccharides is continuously added when digestion due to gastric acid and gastric juice and is diluted.
Simulation gastric digestion device schematic diagram is as shown in Figure 1.Device includes that determinand holds ware 1, hydrochloric acid (HCl) holds ware 2, determinand conveying constant flow pump 3, hydrochloric acid carry constant flow pump 4, stomach reactor 5, magnetic stirring apparatus 6, temperature and pH monitoring system 7, gastric emptying constant flow pump 8, determinand are collected and held ware 9, water-bath control system 10, pH meter 11, thermometer 12.The inside of stomach reactor 5 is reative cell, and outer layer is water-bath layer.Determinand is held ware 1 and is connected with determinand conveying constant flow pump 3 by flexible pipe, and the other end of the constant flow pump 3 of determinand conveying simultaneously stretches into the inside of stomach reactor 5 also by flexible pipe.Hydrochloric acid is held ware 2 and is connected with hydrochloric acid conveying constant flow pump 4 by flexible pipe, and the other end of the constant flow pump 4 of hydrochloric acid conveying simultaneously stretches into the inside of stomach reactor 5 also by flexible pipe.Determinand collection is held ware 9 and is connected with gastric emptying constant flow pump 8 by flexible pipe, and the other end of gastric emptying constant flow pump 8 is also by the hose connection 5 inner bottom outlets to stomach reactor simultaneously.Stomach reactor 5 is placed on magnetic stirring apparatus 6.Temperature and pH monitoring system 7 control to connect pH meter 11 and thermometer 12 by electric wire, and pH meter 11 and thermometer 12 are suspended in the inside of stomach reactor 5.Water-bath control system 10 is connected by flexible pipe 2 water-baths upper and lower with the outer layer of stomach reactor 5 outlet.
Using method: as shown in Figure 1, add the gastric juice of q.s to the inside of stomach reactor 5, the vegetable polysaccharides solution held by determinand in ware 1 carries out stomach simulation digestion by flexible pipe and determinand conveying constant flow pump 3 with the inside of the flow velocity addition stomach reactor 5 of 3.5 ml/min.Open magnetic stirring apparatus 6, with 200
The wriggling of the constant mixing speed simulation stomach of rpm/min, controls the temperature in reactor by water-bath control system 10, and the thermometer 12 connected by temperature and pH monitoring system 7 monitors the internal temperature of stomach reactor 5, keeps constant 37 DEG C.Meanwhile, the pH meter 11 connected by temperature and pH monitoring system 7 monitors the internal pH of stomach reactor 5, and once pH is too high, then adding a certain amount of 0.1 M HCl(flow velocity with hydrochloric acid conveying constant flow pump 4 with constant speed is 1 ml/min) regulate and control, remain that pH is about 2.The time of stomach simulation digestion is 6 h, is simulated the emptying (flow velocity is 4 ml/min) of stomach by gastric emptying constant flow pump 8, is collected in determinand by postdigestive product collection and holds in ware 9, obtains peptic digestion product.Afterwards, by the digestion product collected in boiling water bath a few minutes to go depepsinized activity.
Experimental group 1,2,3 are simulated digestion process in triplicate respectively, detect the digestion situation (the change of molecular weight) of vegetable polysaccharides with liquid phase method, and change by dinitrosalicylic acid system detection reducing sugar amount.
Testing result is as shown in the table:
| Sample | Molecular weight (kDa) | Concentration of reduced sugar(mM) |
| Vegetable polysaccharides before peptic digestion | 1,803.5 ± 78.0 | 0.150 ± 0.006 |
| Vegetable polysaccharides after peptic digestion | 19.5 ± 2.0 | 0.634 ± 0.025 |
As can be seen from the above table, this vegetable polysaccharides is after stomach simulation digestion, and molecular weight significantly reduces, and reducing sugar amount significantly raises, and shows that this vegetable polysaccharides is decomposed in human stomach digests, and glycosides key is interrupted simultaneously.
Embodiment 2.
The stomach simulation digestion of corn starch.
(1) preparation of gastric juice.
Gastric juice is to weigh 35 mg gastric lipases, and 35 mg pepsin join the (preparation of stomach electrolyte solution: weigh 3 g NaCl, 0.5 g KCl, 0.1 g CaCl in 160 g stomach electrolyte2, 0.5 g NaHCO3, with deionized water constant volume in the volumetric flask of 1 L, with the HCl of 0.2 M, electrolyte solution pH is adjusted to 3), it is subsequently adding 2 mL CH3COONa(1 M, pH 5).Magnetic agitation 20 min under room temperature, is adjusted to 2 with the HCl of 0.2 M by pH value of solution, is placed in refrigerator standby.
(2) simulation gastric digestion.
First, the corn starch solution (5 mg/ml) first passing through oral cavity simulation digestion the most in advance is placed in ware.
Then, gastric digestion uses controlled trial method to be grouped as follows.
Experimental group 1: add gastric acid in analog, gastric juice and corn starch solution carry out stomach simulation digestion.
Experimental group 2: add gastric acid in analog, gastric juice and water (with the corn starch solution same volume in experimental group 1) carry out stomach simulation digestion, and this experimental group is for removing material own in gastric acid and gastric juice for analyzing the interference of corn starch gastric digestion situation.
Experimental group 3: adding corn starch solution in analog and water (with the gastric acid in experimental group 1 and gastric juice same volume) carries out stomach simulation digestion, this experimental group is for removing the impact that corn starch is continuously added when digestion due to gastric acid and gastric juice and is diluted.
Simulation gastric digestion device schematic diagram is as shown in Figure 1.Device includes that determinand holds ware 1, hydrochloric acid (HCl) holds ware 2, determinand conveying constant flow pump 3, hydrochloric acid carry constant flow pump 4, stomach reactor 5, magnetic stirring apparatus 6, temperature and pH monitoring system 7, gastric emptying constant flow pump 8, determinand are collected and held ware 9, water-bath control system 10, pH meter 11, thermometer 12.The inside of stomach reactor 5 is reative cell, and outer layer is water-bath layer.Determinand is held ware 1 and is connected with determinand conveying constant flow pump 3 by flexible pipe, and the other end of the constant flow pump 3 of determinand conveying simultaneously stretches into the inside of stomach reactor 5 also by flexible pipe.Hydrochloric acid is held ware 2 and is connected with hydrochloric acid conveying constant flow pump 4 by flexible pipe, and the other end of the constant flow pump 4 of hydrochloric acid conveying simultaneously stretches into the inside of stomach reactor 5 also by flexible pipe.Determinand collection is held ware 9 and is connected with gastric emptying constant flow pump 8 by flexible pipe, and the other end of gastric emptying constant flow pump 8 is also by the hose connection 5 inner bottom outlets to stomach reactor simultaneously.Stomach reactor 5 is placed on magnetic stirring apparatus 6.Temperature and pH monitoring system 7 control to connect pH meter 11 and thermometer 12 by electric wire, and pH meter 11 and thermometer 12 are suspended in the inside of stomach reactor 5.Water-bath control system 10 is connected by flexible pipe 2 water-baths upper and lower with the outer layer of stomach reactor 5 outlet.
Using method: as shown in Figure 1, add the gastric juice of q.s to the inside of stomach reactor 5, the corn starch solution held by determinand in ware 1 carries out stomach simulation digestion by flexible pipe and determinand conveying constant flow pump 3 with the inside of certain flow velocity (less than 3 ml/min) addition stomach reactor 5.Open magnetic stirring apparatus 6, with 300
The wriggling of the constant mixing speed simulation stomach of rpm/min, controls the temperature in reactor by water-bath control system 10, and the thermometer 12 connected by temperature and pH monitoring system 7 monitors the internal temperature of stomach reactor 5, keeps constant 37 DEG C.Meanwhile, the pH meter 11 connected by temperature and pH monitoring system 7 monitors the internal pH of stomach reactor 5, and once pH is too high, then adding a certain amount of 0.1 M HCl(flow velocity with hydrochloric acid conveying constant flow pump 4 with constant speed is 2 ml/min) regulate and control, remain that pH is about 2.The time of stomach simulation digestion is 5 h, is simulated the emptying (flow velocity is 4 ml/min) of stomach by gastric emptying constant flow pump 8, is collected in determinand by postdigestive product collection and holds in ware 9, obtains peptic digestion product.Afterwards, by the digestion product collected in boiling water bath a few minutes to go depepsinized activity.
Experimental group 1,2,3 are simulated digestion process in triplicate respectively, detect the digestion situation (the change of molecular weight) of corn starch with liquid phase method, and change by dinitrosalicylic acid system detection reducing sugar amount.
Testing result is as shown in the table:
| Sample Product | Molecular weight (kDa) | Concentration of reduced sugar (mM) |
| Corn starch before peptic digestion | 162.7 ± 3.2 | 0.634 ± 0.025 |
| Corn starch after peptic digestion | 128.7 ± 3.2 | 0.708 ± 0.030 |
As can be seen from the above table, corn starch is after stomach simulation digestion, and molecular weight fractions reduces, and reducing sugar amount has certain rising, shows that this corn starch is decomposed in human stomach digests on a small quantity, and the most a small amount of glycosidic bond is interrupted.
Enforcement case 3.
The stomach simulation digestion of soybean protein.
(1) preparation of gastric juice.
Gastric juice is to weigh 35 mg gastric lipases, and 35 mg pepsin join the (preparation of stomach electrolyte solution: weigh 3 g NaCl, 0.5 g KCl, 0.1 g CaCl in 160 g stomach electrolyte2, 0.5 g NaHCO3, with deionized water constant volume in the volumetric flask of 1 L, with the HCl of 0.2 M, electrolyte solution pH is adjusted to 3), it is subsequently adding 2 mL CH3COONa(1 M, pH 5).Magnetic agitation 20 min under room temperature, is adjusted to 2 with the HCl of 0.2 M by pH value of solution, is placed in refrigerator standby.
(2) simulation gastric digestion.
First, the soy bean proteinous soln (5 mg/ml) first passing through oral cavity simulation digestion the most in advance is placed in ware.
Then, gastric digestion uses controlled trial method to be grouped as follows.
Experimental group 1: add gastric acid in analog, gastric juice and soy bean proteinous soln carry out stomach simulation digestion.
Experimental group 2: add gastric acid in analog, gastric juice and water (with the soy bean proteinous soln same volume in experimental group 1) carry out stomach simulation digestion, and this experimental group is for removing material own in gastric acid and gastric juice for analyzing the interference of soybean protein gastric digestion situation.
Experimental group 3: adding soy bean proteinous soln in analog and water (with the gastric acid in experimental group 1 and gastric juice same volume) carries out stomach simulation digestion, this experimental group is for removing the impact that soybean protein is continuously added when digestion due to gastric acid and gastric juice and is diluted.
Simulation gastric digestion device schematic diagram is as shown in Figure 1.Device includes that determinand holds ware 1, hydrochloric acid (HCl) holds ware 2, determinand conveying constant flow pump 3, hydrochloric acid carry constant flow pump 4, stomach reactor 5, magnetic stirring apparatus 6, temperature and pH monitoring system 7, gastric emptying constant flow pump 8, determinand are collected and held ware 9, water-bath control system 10, pH meter 11, thermometer 12.The inside of stomach reactor 5 is reative cell, and outer layer is water-bath layer.Determinand is held ware 1 and is connected with determinand conveying constant flow pump 3 by flexible pipe, and the other end of the constant flow pump 3 of determinand conveying simultaneously stretches into the inside of stomach reactor 5 also by flexible pipe.Hydrochloric acid is held ware 2 and is connected with hydrochloric acid conveying constant flow pump 4 by flexible pipe, and the other end of the constant flow pump 4 of hydrochloric acid conveying simultaneously stretches into the inside of stomach reactor 5 also by flexible pipe.Determinand collection is held ware 9 and is connected with gastric emptying constant flow pump 8 by flexible pipe, and the other end of gastric emptying constant flow pump 8 is also by the hose connection 5 inner bottom outlets to stomach reactor simultaneously.Stomach reactor 5 is placed on magnetic stirring apparatus 6.Temperature and pH monitoring system 7 control to connect pH meter 11 and thermometer 12 by electric wire, and pH meter 11 and thermometer 12 are suspended in the inside of stomach reactor 5.Water-bath control system 10 is connected by flexible pipe 2 water-baths upper and lower with the outer layer of stomach reactor 5 outlet.
Using method: as shown in Figure 1, add the gastric juice of q.s to the inside of stomach reactor 5, the soy bean proteinous soln held by determinand in ware 1 carries out stomach simulation digestion by flexible pipe and determinand conveying constant flow pump 3 with the inside of certain flow velocity (less than 4 ml/min) addition stomach reactor 5.Open magnetic stirring apparatus 6, with 200
The wriggling of the constant mixing speed simulation stomach of rpm/min, controls the temperature in reactor by water-bath control system 10, and the thermometer 12 connected by temperature and pH monitoring system 7 monitors the internal temperature of stomach reactor 5, keeps constant 37 DEG C.Meanwhile, the pH meter 11 connected by temperature and pH monitoring system 7 monitors the internal pH of stomach reactor 5, and once pH is too high, then adding a certain amount of 0.2 M HCl(flow velocity with hydrochloric acid conveying constant flow pump 4 with constant speed is 1 ml/min) regulate and control, remain that pH is about 2.The time of stomach simulation digestion is 5 h, is simulated the emptying (flow velocity is 4 ml/min) of stomach by gastric emptying constant flow pump 8, is collected in determinand by postdigestive product collection and holds in ware 9, obtains peptic digestion product.Afterwards, by the digestion product collected in boiling water bath a few minutes to go depepsinized activity.
Experimental group 1,2,3 are simulated digestion process in triplicate respectively, by the digestion situation (the change of molecular weight) of liquid phase method detection soybean protein.
Testing result is as shown in the table:
| Sample Product | Molecular weight (kDa) |
| Soybean protein before peptic digestion | 30.6 ± 4.1 |
| Soybean protein after peptic digestion | 5.8 ± 1.2 |
As can be seen from the above table, soybean protein is after stomach simulation digestion, and molecular weight significantly reduces, and shows that this soybean protein is significantly decomposed in human stomach digests.
Claims (1)
1. simulating a using method for the device of nutrient substance gastric digestion, device includes that determinand holds ware (1), hydrochloric acid holds ware (2), determinand conveying constant flow pump (3), hydrochloric acid carry constant flow pump (4), stomach reactor (5), magnetic stirring apparatus (6), temperature and pH monitoring system (7), gastric emptying constant flow pump (8), determinand are collected and held ware (9), water-bath control system (10), pH meter (11), thermometer (12)；The inside of stomach reactor (5) is reative cell, and outer layer is water-bath layer；Determinand is held ware (1) and is connected with determinand conveying constant flow pump (3) by flexible pipe, and the other end of determinand conveying constant flow pump (3) stretches into the inside of stomach reactor (5) by flexible pipe；Hydrochloric acid is held ware (2) and is connected with hydrochloric acid conveying constant flow pump (4) by flexible pipe, and the other end of hydrochloric acid conveying constant flow pump (4) stretches into the inside of stomach reactor (5) by flexible pipe；Determinand collection is held ware (9) and is connected with gastric emptying constant flow pump (8) by flexible pipe, and the other end of gastric emptying constant flow pump (8) is exported by the hose connection inner bottom to stomach reactor (5)；Stomach reactor (5) is placed on magnetic stirring apparatus (6)；Temperature and pH monitoring system (7) connect pH meter (11) and thermometer (12) respectively, and pH meter (11) and thermometer (12) are suspended in the inside of stomach reactor (5)；Water-bath control system (10) is connected by flexible pipe 2 water-baths upper and lower with the outer layer of stomach reactor (5) outlet；It is characterized in that:
Add the gastric juice of q.s to the inside of stomach reactor (5), determinand is held the determinand solution in ware (1) or dispersion liquid and carries out stomach simulation digestion by determinand conveying constant flow pump (3) with the inside of≤5 ml/min flow velocitys addition stomach reactor (5)；Open magnetic stirring apparatus (6), wriggling with the constant mixing speed simulation stomach of 200-300 rpm/min, the temperature in reactor is controlled by water-bath control system (10), and the internal temperature of the thermometer (12) monitoring stomach reactor (5) connected by temperature and pH monitoring system (7), keep constant 37 DEG C；Simultaneously, by the internal pH of the pH meter (11) monitoring stomach reactor (5) that temperature and pH monitoring system (7) connect, once pH is too high, then carry constant flow pump (4) with hydrochloric acid and regulate and control with the constant speed addition 0.1-0.2 M hydrochloric acid of 1-2 ml/min, remain that pH is 2-3；The time of stomach simulation digestion is 5-6 h, by gastric emptying constant flow pump (8) with the emptying of 4-6 ml/min flow velocity simulation stomach, is collected in determinand by postdigestive product collection and holds in ware (9)；Afterwards, by the digestion product collected in boiling water bath a few minutes to go depepsinized activity；Simulation digestion process in triplicate, detects the digestion situation of test substance with liquid phase method.
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| CN103675205A (en) | 2014-03-26 |
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