CN208933355U - Sterilization culture medium stirs dispensing apparatus - Google Patents

Sterilization culture medium stirs dispensing apparatus Download PDF

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Publication number
CN208933355U
CN208933355U CN201821371023.3U CN201821371023U CN208933355U CN 208933355 U CN208933355 U CN 208933355U CN 201821371023 U CN201821371023 U CN 201821371023U CN 208933355 U CN208933355 U CN 208933355U
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China
Prior art keywords
culture medium
dispensing apparatus
agitator tank
packing
chamber
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CN201821371023.3U
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Chinese (zh)
Inventor
潘素华
安文佳
李伟明
龙晓婷
冯旭祥
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Comprehensive Technical Service Center Of Panyu Entry Exit Inspection And Quarantine Bureau
Inspection and Quarantine Technology Center of Guangdong Entry Exit Inspection and Quarantine Bureau
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Comprehensive Technical Service Center Of Panyu Entry Exit Inspection And Quarantine Bureau
Inspection and Quarantine Technology Center of Guangdong Entry Exit Inspection and Quarantine Bureau
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Abstract

The utility model provides sterilization culture medium and stirs dispensing apparatus, belonging to field of microbial culture technology includes agitator tank, agitator tank is from top to bottom successively arranged teeter chamber, transfer chamber and packing room, the support column with idler wheel is connected with below agitator tank, it dispenses indoor top and is equipped with the diversion mechanism connecting with transfer chamber, splicing pedestal is correspondingly arranged below diversion mechanism, splicing pedestal is placed on above elastomer block, shake machine is equipped with below elastomer block, sterilamp is respectively equipped in packing chamber interior walls, blast pipe, and packing room side offers switching door, the equipment whole process sterilization of the utility model operates, there are bubbles in avoidable culture medium, the solute syncretizing effect of culture medium is good, culture medium packing is high-efficient.

Description

Sterilization culture medium stirs dispensing apparatus
Technical field
The utility model belongs to field of microbial culture technology, and in particular to a kind of stirring packing of sterilization culture medium is set It is standby.
Background technique
With biotechnology fast-developing, more and more producers cultivate plant children using tissue culture technique scale Seedling, such as banana, roxburgh anoectochilus terminal bud and dendrobium candidum.Before tissue cultures, generally requires and culture medium is stirred, is dispensed in batches And autoclave sterilization.
The prior art provides a variety of culture medium stirring dispensing apparatus, such as Chinese utility model granted patent, application number: CN201620982032.0, the utility model disclose shell plant tissue culture media stirring subpackage apparatus, belong to Plant Tissue Breeding Technical field comprising agitator, the top of the agitator are equipped with driving equipment, and the driving equipment is connected by shaft coupling There is mixing plant, water-bath is equipped with below the agitator, agitator bottom is connected with guide pipe, and described guide pipe one end extends On the outside of to water-bath and multiple packing pipes are connected with, solenoid valve, flowmeter are installed on the packing pipe, packing pipe discharge end Lower section is equipped with ultrasonic oscillation device, and the solenoid valve and flowmeter are electrically connected with control equipment.The utility model is stirred by improving The construction of subpackage apparatus is mixed to improve the packing efficiency of culture medium, while the culture medium in culture bottle being avoided to form bubble, still Syncretizing effect also has room for promotion between the solute for the culture medium that the device is prepared.
Utility model content
The purpose of this utility model is to provide a kind of whole sterilizations to operate, and there are bubble, trainings in avoidable culture medium The solute syncretizing effect for supporting base is good, and culture medium dispenses high-efficient sterilization culture medium stirring dispensing apparatus.
The technical solution that the utility model is taken to achieve the above object are as follows: sterilization culture medium stirs dispensing apparatus, Including agitator tank, agitator tank is from top to bottom successively arranged teeter chamber, transfer chamber and packing room, is connected with band idler wheel below agitator tank Support column, dispense indoor top and be equipped with diversion mechanism connect with transfer chamber, diversion mechanism lower section is correspondingly arranged on splicing base Seat, splicing pedestal are placed on above elastomer block, and shake machine is equipped with below elastomer block, are dispensed to be respectively equipped in chamber interior walls and be gone out Bacterium lamp, blast pipe, and dispense room side and offer switching door, the present apparatus dispenses culture medium solute using diversion mechanism, Culture medium packing efficiency is greatly improved, while dither operation is carried out to the culture medium after packing, promotes the fusion effect between solute Fruit effectively excludes the gas between culture medium solute, and culture medium is dispensed and gone compared to traditional this equipment of subpackage apparatus Except the gas efficiency between solute is higher, and Cheng Qian was dispensed in culture medium, sterilizing behaviour is carried out to dispensing apparatus using sterilamp Avoid in medium container there are bacterium cause culture medium pollute or decomposed metamorphic, and dispense and culture medium was shaken Sterile wind is blown to packing interior using blast pipe after journey to promote to dispense indoor air flowing, and packing room air is avoided not flow The logical effect for influencing culture medium.
Preferably, splicing pedestal includes pedestal, susceptor surface offers the putting hole with diversion mechanism corresponding matching, puts Set the clamping plate that two pieces of slips are placed on the hole wall in hole, the horizontally disposed bolt having for pushing clamping plate in pedestal side, splicing Pedestal places container for placing culture medium, and can play fixed function to container according to container size bolt and clamping plate, Keep container entrance corresponding with the discharge port of the shunt pipe of diversion mechanism always.
Preferably, diversion mechanism includes the shunting general pipeline connecting with transfer chamber, the lower section shunting general pipeline is evenly equipped with and splicing The corresponding shunt pipe of base position, is connected separately with valve and flowmeter on shunt pipe, and diversion mechanism can be to stirring and high Temperature treated culture medium solute shunting is put into medium container, and the amount of being put into can be controlled by valve, flowmeter and control panel It realizes, the entire process that dispenses is full-automatic, is not necessarily to artificial monitoring, and dispensed loading amount is accurate, and the culture medium component of packing is effectively ensured And effect.
It is connected to feed pipe and exhaust pipe preferably, wearing above teeter chamber, is equipped in stirring by being placed in outside teeter chamber Motor-driven blender, upper wall is connected with gas pressure detector in teeter chamber, and tracheae, gas are connected in teeter chamber's upper portion side wall Pipe is connect with the air pump outside agitator tank, and air pump side is connected with sterilizing filter, can be to input high pressure sterile gas in teeter chamber Body promotes to stir rapid fusion between indoor culture medium solute, while the setting of exhaust pipe and gas pressure detector using gas It can avoid the excessively high existing security risk of stirring room pressure and the influence to culture medium solute, sterilizing filter be exportable sterile Gas is avoided leading to the culture medium solute pollution of preparation there are bacterium in air or be decomposed.
Preferably, blender includes the stirring frame of two diamond shapeds, it is connected with several stirring rods vertically in stirring frame, stirs It mixes and is evenly equipped at least two elastomers on bar, elastomer is connected by the sliding plate and sliding plate upper and lower surface that can be slid on stirring rod The blender of the bullet block composition connect, above structure can realize the effect quickly broken up and merged to raw material in culture medium whipping process Fruit, in blender operation process sliding plate repeatedly on stirring rod sliding and two sliding plates collide when, due to bullet block Being arranged can be such that the direction of motion of two sliding plates moves backward, to blender to the stirring track of culture medium raw material in rotary course Variation is constantly generated, the rapid fusion of the solute of culture medium is beneficial to, improves stirring efficiency and effect.
Preferably, transfer chamber includes for being connected to teeter chamber and dispensing the U-shape transfer tube of room, transfer chamber upper side is set There is water inlet pipe, side-lower is equipped with drainpipe, and transmits chamber interior walls and be equipped with temperature detector, completes using fluid temperature to stirring Culture medium solute carry out high-temperature sterilization processing, the transfer tube of U-shape can effectively extend culture medium solute in the stop of transfer chamber The sterilization effect of culture medium solute is effectively ensured in time, and temperature detector can monitor the indoor fluid temperature of transmission in real time, If fluid temperature reduces, high-temp liquid is inputted from water inlet pipe into transfer chamber by the indoor liquid of drainpipe discharge transmission again and is held The continuous culture medium solute in transfer tube carries out high-temperature sterilization.
Preferably, being connected with control panel on agitator tank side wall, for controlling each component in this equipment, only pass through control The operations such as the gas in stirring, sterilizing, packing and removal culture medium to culture medium raw material can be completed in panel processed, realize automatic Change operation.
Compared with prior art, the utility model has the following beneficial effects: 1) promoting the culture medium of stirring using aseptic gas Rapid fusion between solute;2) blender designed constantly generates change to the stirring track of culture medium raw material in rotary course Change, be beneficial to the rapid fusion of the solute of culture medium, improves stirring efficiency and effect;3) using fluid temperature to culture medium solute High-temperature sterilization processing is carried out, and sterilization effect is good;4) this equipment can realize stirring to culture medium raw material, sterilizing, packing and The operation of gas in culture medium is removed, entire operation process is sterilization operation, effectively avoids germ contamination culture medium or training Support base decomposed metamorphic;5) gas between solute culture medium is dispensed and is removed compared to traditional this equipment of subpackage apparatus to imitate Rate is higher.
The utility model uses the sterilization culture medium that above-mentioned technical proposal provides and stirs dispensing apparatus, compensates for existing The deficiency of technology, reasonable design, easy operation.
Detailed description of the invention
Fig. 1 is that the utility model sterilization culture medium stirs dispensing apparatus schematic diagram;
Fig. 2 is blender schematic diagram;
Fig. 3 is elastomer schematic diagram;
Fig. 4 is splicing pedestal schematic diagram;
Fig. 5 is diversion mechanism schematic diagram.
Description of symbols: 1. agitator tanks;101. support column;2. teeter chamber;201. feed pipe;202. motor;203. gas Press detector;204. exhaust pipe;205. observation window;3. transfer chamber;301. temperature detector;302. transfer tube;303. drainpipe; 304. water inlet pipe;4. dispensing room;401. blast pipe;402. sterilamp;403. splicing pedestals;403a. pedestal;403b. putting hole; 403c. clamping plate;403d. bolt;404. switching door;405. elastomer block;406. shake machines;5. blender;501. stirring rod;502. Stirring frame;503. elastomer;503a. sliding plate;503b. bullet block;6. control panel;7. diversion mechanism;701. shunt general pipeline; 702. shunt pipe;703. flowmeter;704. valve;8. air pump;801. tracheae;802. sterilizing filter.
Specific embodiment
The utility model is described in further detail with attached drawing with reference to embodiments:
Embodiment 1:
As shown in Figure 1, sterilization culture medium stirs dispensing apparatus, including agitator tank 1, agitator tank 1 are from top to bottom successively set There are teeter chamber 2, transfer chamber 3 and packing room 4, the support column 101 with idler wheel is connected with below agitator tank 1, packing 4 internal upper part of room is set There is the diversion mechanism 7 connecting with transfer chamber 3, splicing pedestal 403 is correspondingly arranged below diversion mechanism 7, splicing pedestal 403 is placed Above elastomer block 405, it is equipped with shake machine 406 below elastomer block 405, dispenses and is respectively equipped with sterilamp on 4 inner wall of room 402, blast pipe 401, and dispense 4 side of room and offer switching door 404, the present apparatus using diversion mechanism 7 to culture medium solute into Row packing greatly improves culture medium packing efficiency, while carrying out dither operation to the culture medium after packing, promotes between solute Syncretizing effect effectively excludes the gas between culture medium solute, compared to traditional this equipment of subpackage apparatus for culture medium point Gas efficiency between dress and removal solute is higher, and dispenses Cheng Qian using sterilamp 402 to dispensing apparatus in culture medium Carry out disinfecting action avoid in medium container there are bacterium cause culture medium pollute or decomposed metamorphic, and packing and to training The air flowing blown in sterile wind promotion packing room 4 in room 4 is dispensed using 401 pairs of blast pipe after supporting base vibration process, is avoided Dispensing atmosphere draught-free in room 4 influences the effect of culture medium.
It is worn above teeter chamber 2 and is connected to feed pipe 201 and exhaust pipe 204, be equipped in teeter chamber 2 by being placed in outside teeter chamber 2 The blender 5 that the motor 202 in portion drives, upper wall is connected with gas pressure detector 203 in teeter chamber 2, connects in 2 upper portion side wall of teeter chamber It is connected to tracheae 801, tracheae 801 is connect with the air pump 8 outside agitator tank 1, and 8 side of air pump is connected with sterilizing filter 802, can be right High pressure sterile gas is inputted in teeter chamber 2, promotes rapid fusion between the culture medium solute in teeter chamber 2 using gas, simultaneously The setting of exhaust pipe 204 and gas pressure detector 203 can avoid the excessively high existing security risk of air pressure in teeter chamber 2 and to culture medium The influence of solute, the exportable aseptic gas of sterilizing filter 802 avoid in air there are bacterium cause preparation culture medium solute Pollution is decomposed.
Transfer chamber 3 includes for being connected to teeter chamber 2 and dispensing the U-shape transfer tube 302 of room 4, and 3 upper side of transfer chamber is equipped with Water inlet pipe 304, side-lower is equipped with drainpipe 303, and 3 inner wall of transfer chamber is equipped with temperature detector 301, utilizes fluid temperature pair The culture medium solute that stirring is completed carries out high-temperature sterilization processing, and the transfer tube 302 of U-shape can effectively extend culture medium solute and pass The sterilization effect of culture medium solute is effectively ensured in the residence time of defeated room 3, and temperature detector 301 can monitor transmission in real time The liquid in transfer chamber 3 is discharged again by water inlet pipe 304 by drainpipe 303 if fluid temperature is reduced in fluid temperature in room 3 High-temp liquid is inputted into transfer chamber 3, and high-temperature sterilization persistently is carried out to the culture medium solute in transfer tube 302.
It is connected with control panel 6 on 1 side wall of agitator tank, for controlling each component in this equipment, only passes through control panel 6 The operations such as the gas in stirring, sterilizing, packing and the removal culture medium to culture medium raw material can be completed, realize automation behaviour Make.
Embodiment 2:
The present embodiment advanced optimizes scheme on the basis of embodiment 1 are as follows: as shown in figure 4, splicing pedestal 403 includes bottom Seat 403a, the surface pedestal 403a offers the putting hole 403b with 7 corresponding matching of diversion mechanism, puts on the hole wall of putting hole 403b It is equipped with the clamping plate 403c of two pieces of slips, the horizontally disposed bolt 403d having for pushing clamping plate 403c in the side pedestal 403a connects Material pedestal 403 places container for placing culture medium, and can be according to container size bolt 403d and clamping plate 403c to container Fixed function is played, keeps container entrance corresponding with the discharge port of shunt pipe 702 of diversion mechanism 7 always.
Embodiment 3:
The present embodiment advanced optimizes scheme on the basis of embodiment 1 are as follows: as shown in figure 5, diversion mechanism 7 includes and biography The shunting general pipeline 701 that defeated room 3 connects is shunted and is evenly equipped with shunt pipe corresponding with 403 position of splicing pedestal below general pipeline 701 702, valve 704 and flowmeter 703 are connected separately on shunt pipe 702, after diversion mechanism 7 can be to stirring and high-temperature process The shunting of culture medium solute is put into medium container, and the amount of being put into can control reality by valve 704, flowmeter 703 and control panel 6 Existing, the entire process that dispenses is full-automatic, is not necessarily to artificial monitoring, and dispensed loading amount is accurate, be effectively ensured packing culture medium component and Effect.
Embodiment 4:
The present embodiment advanced optimizes scheme on the basis of embodiment 1 are as follows: as shown in Figure 2,3, blender 5 includes two The stirring frame 502 of diamond shaped is connected with several stirring rods 501 in stirring frame 502 vertically, is evenly equipped at least two on stirring rod 501 A elastomer 503, elastomer 503 are connected by the sliding plate 503a that can be slid on stirring rod 501 with 503 upper and lower surface of sliding plate Bullet block 503b composition, the blender 5 of above structure quickly can break up and merge to raw material realization in culture medium whipping process Effect, in 5 operation process of blender sliding plate 503a repeatedly on stirring rod 501 sliding and two sliding plates 503 collide When hitting, since the setting of bullet block 503b can be such that the direction of motion of two sliding plates 503 moves backward, to blender 5 in rotary course In variation is constantly generated to the stirring track of culture medium raw material, be beneficial to the rapid fusion of the solute of culture medium, improve stirring effect Rate and effect.
Embodiment 5:
The sterilization culture medium of the utility model stirs dispensing apparatus in actual use: originally being set by the control of control panel 6 Each component in standby carries out parameter setting, and culture medium raw material is poured into feed pipe 201 and is carried out by blender 5 to culture medium raw material Air pump 8, sterilizing filter 802 and tracheae 801 promote between culture medium solute input high pressure gas in teeter chamber 2 simultaneously for stirring Fusion, culture medium solute passes through transfer chamber 3 again and carries out high-temperature sterilization arrival packing room 4, by diversion mechanism 7 and splicing pedestal 403 The packing to culture medium is completed, the culture medium vibration finally completed by 406 pairs of shake machine packing operates the gas in discharge culture medium Body.
The prior art of routine techniques dawn known to those skilled in the art in above-described embodiment, selected electrical part Part is that conventional electrical component is sold in market, and concrete model and power need to be selected according to equipment actual conditions.
Specific embodiment described herein is only to give an example to the utility model.The affiliated skill of the utility model The technical staff in art field can make various modifications or additions to the described embodiments or using similar side Formula substitution, but without departing from the utility model or beyond the scope of the appended claims.

Claims (7)

1. sterilization culture medium stirs dispensing apparatus, including agitator tank (1), agitator tank (1) are from top to bottom successively arranged teeter chamber (2), transfer chamber (3) and packing room (4), agitator tank (1) lower section are connected with the support column (101) with idler wheel, it is characterised in that: institute It states packing room (4) internal upper part and is equipped with the diversion mechanism (7) connecting with transfer chamber (3), be correspondingly arranged below the diversion mechanism (7) Have splicing pedestal (403), the splicing pedestal (403) is placed on above elastomer block (405), matches below the elastomer block (405) Conjunction is provided with shake machine (406), sterilamp (402), blast pipe (401) is respectively equipped on packing room (4) inner wall, and dispense Room (4) side offers switching door (404).
2. sterilization culture medium according to claim 1 stirs dispensing apparatus, it is characterised in that: the splicing pedestal It (403) include pedestal (403a), pedestal (403a) surface offers and the putting hole of diversion mechanism (7) corresponding matching (403b) is placed with the clamping plate (403c) of two pieces of slips, pedestal (403a) side on the hole wall of the putting hole (403b) Side is horizontally disposed to be had for pushing the bolt of clamping plate (403c) (403d).
3. sterilization culture medium according to claim 1 stirs dispensing apparatus, it is characterised in that: the diversion mechanism (7) Including the shunting general pipeline (701) being connect with transfer chamber (3), it is evenly equipped with and splicing pedestal (403) below the shunting general pipeline (701) The corresponding shunt pipe in position (702) is connected separately with valve (704) and flowmeter (703) on the shunt pipe (702).
4. sterilization culture medium according to claim 1 stirs dispensing apparatus, it is characterised in that: on the teeter chamber (2) Side, which is worn, is connected to feed pipe (201) and exhaust pipe (204), is equipped in teeter chamber (2) by being placed in the external motor in teeter chamber (2) (202) blender (5) driven, teeter chamber (2) interior upper wall are connected with gas pressure detector (203), teeter chamber (2) upper side It is connected on wall tracheae (801), the tracheae (801) air pump (8) external with agitator tank (1) is connect, air pump (8) side It is connected with sterilizing filter (802).
5. sterilization culture medium according to claim 4 stirs dispensing apparatus, it is characterised in that: blender (5) packet The stirring frame (502) of two diamond shapeds is included, is connected with several stirring rods (501), the stirring vertically in the stirring frame (502) It is evenly equipped on bar (501) at least two elastomers (503), the elastomer (503) is by the cunning that can slide on stirring rod (501) Move bullet block (503b) composition that plate (503a) is connected with sliding plate (503a) upper and lower surface.
6. sterilization culture medium according to claim 1 stirs dispensing apparatus, it is characterised in that: transfer chamber (3) packet It includes for being connected to teeter chamber (2) and dispensing the U-shape transfer tube (302) of room (4), transfer chamber (3) upper side is equipped with water inlet It manages (304), side-lower is equipped with drainpipe (303), and transfer chamber (3) inner wall is equipped with temperature detector (301).
7. sterilization culture medium according to claim 1 stirs dispensing apparatus, it is characterised in that: agitator tank (1) side Control panel (6) are connected on wall.
CN201821371023.3U 2018-08-24 2018-08-24 Sterilization culture medium stirs dispensing apparatus Active CN208933355U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201821371023.3U CN208933355U (en) 2018-08-24 2018-08-24 Sterilization culture medium stirs dispensing apparatus

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201821371023.3U CN208933355U (en) 2018-08-24 2018-08-24 Sterilization culture medium stirs dispensing apparatus

Publications (1)

Publication Number Publication Date
CN208933355U true CN208933355U (en) 2019-06-04

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111704983A (en) * 2020-06-23 2020-09-25 扬州云朵医疗科技有限公司 Automatic preparation facilities of culture medium based on PLC control
WO2022205992A1 (en) * 2021-04-01 2022-10-06 湖北文理学院 Culture medium manufacturing system

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111704983A (en) * 2020-06-23 2020-09-25 扬州云朵医疗科技有限公司 Automatic preparation facilities of culture medium based on PLC control
WO2022205992A1 (en) * 2021-04-01 2022-10-06 湖北文理学院 Culture medium manufacturing system

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