WO2011006285A1

WO2011006285A1 - Inoculation method for edible fugus seed and culture method for mycelium and device thereof

Info

Publication number: WO2011006285A1
Application number: PCT/CN2009/001518
Authority: WO
Inventors: 徐寿海
Original assignee: Xu Shouhai
Priority date: 2009-07-13
Filing date: 2009-12-29
Publication date: 2011-01-20
Also published as: CN101822168A; CN101822168B

Abstract

An inoculation method for edible fungus seed comprises the following steps: loading culture medium material into a container (2), stirring the culture medium material by a stirring device provided on the container, inducing steam into the container for steam sterilizing, cooling after sterilizing, and inoculating the fungus seed into the container, and at the same time starting the stirring device on the container to stir. A culture method for edible fungus mycelium comprises the following steps: inoculating according to the above inoculation method, and culturing mycelium. An inoculation or culture device for implementing the above inoculation and culture method comprises a container, a stirring device and some inlets and outlets provided on the container. The method and device can shorten the process flow and reduce the culture cost of the edible fungus.

Description

Inoculation of edible fungus strains and hyphae culture method and device Technical field

The present invention relates to the cultivation of edible fungi, especially the inoculation method of edible fungi; the present invention also relates to a method for cultivating seed-feeding fungus hyphae; the present invention also relates to a device for realizing the above-mentioned edible fungus inoculation or hyphae culture.

Background technique

The cultivation of edible fungi can be divided into cultivation period and growth period. The cultivation period is divided into the pre-hyphae culture and the hyphal post-mature cultivation period; the growth period is divided into the early growth and the late growth. The early growth is to cultivate the edible mushroom stalk, and the later growth is from the stalk stage to the edible fungus. Reproductive maturity.

The culture method of inoculation and pre-hyphae culture in the prior art is divided into the following 5 steps:

1. Mixing: Stir the culture medium evenly according to the required humidity;

2. Bottling (bag): Put the stirred medium material in a culture bottle (bag), compact it, and punch a number of pores on the medium material, and then press the bottle cap;

3. Sterilization: transport the packed culture bottle (bag) to a sterilizer for sterilization, and cool it in a clean environment after sterilization;

4. Inoculation: Put the bacteria into the sterilized culture bottle (bag);

5. Cultivation of hyphae: culture under the conditions of cleanness, temperature control, humidity control, and CO ₂ concentration until the culture bottle is full of hyphae; because the strains are inoculated at the mouth of the culture bottle when they are connected, Therefore, the growth of mycelium gradually grows from the mouth of the bottle to the bottom of the bottle to cover the entire culture bottle (also called food); the period from the beginning of the growth of the mycelium to fullness in this step is also called the food period.

After the feeding period is over, the hyphae will enter the post-maturing culture period.

The disadvantages of the inoculation and pre-hyphae culture culture methods in the prior art are:

1. The feeding period is long and the cultivation efficiency is low. According to different strains, the cultivation period of edible fungi will be different. The cultivation period of Pleurotus eryngii is about 35 days. Among them: the culture period of the hyphae at the early stage is about 25 days, and the culture period of the hyphae at the later maturity is about 10 days. It can be seen from this that, in the prior art, the early mycelial culture of edible fungi takes a lot of time, while the first four steps (mixing, bottling, sterilization, and inoculation) of the early mycelial culture are time-consuming. Generally it is about 1 day, basically negligible, because Almost all of the 25 days required are spent in the feeding period of step 5. This is mainly caused by the growth of mycelia from the bottle mouth to the bottom of the bottle to cover the entire culture bottle. If the feeding period is too long, the cultivation efficiency will be low. Second, the cultivation cost is high. The cost of training is mainly reflected in two aspects: one is the cost of equipment; the other is the cost of energy consumption to maintain the cultivation environment. The steps of mixing, bottling, sterilization and inoculation all require certain equipment and a demanding environment. Moreover, due to the long feeding period, the cultivation environment during the feeding period needs to be clean, temperature controlled, humidity controlled, and CO ₂ controlled. To maintain the cultivation environment requires a lot of equipment and energy, so how to shorten the feeding period It is a major problem that the existing technology needs to solve in order to save energy and cost.

3. The probability of bacterial infection is high, and the industry risk is high. Since the five steps of mixing, bottling, sterilizing, inoculating and cultivating mycelium are all completed in different settings, it is necessary to implement the connection between each step. However, in the inoculation and the early stage of mycelium culture after sterilization, the strains are particularly susceptible to infection, and the equipment connection process between the steps greatly increases the chance of infection of the strains, and the infection of the strains will be directly affected. Leading to the failure of edible fungus cultivation.

Summary of the invention

The technical problem to be solved by the present invention is to address the shortcomings of the prior art and provide a method for inoculating edible fungi strains that requires relatively simple equipment and can shorten the mycelial culture time after inoculation.

Another technical problem to be solved by the present invention is to provide two methods for cultivating edible fungus mycelium that are short in time and can greatly save cultivation energy and cost.

Another technical problem to be solved by the present invention is to provide a device for realizing the above-mentioned edible fungus seed inoculation method or edible fungus mycelium culture method.

The method and device of the invention are suitable for the inoculation and cultivation of liquid strains and solid strains.

The technical problem to be solved by the present invention is achieved through the following technical solutions. The present invention is a method for inoculating edible fungi strains, and its characteristic is that the steps are as follows:

(1) Loading: Pack the culture medium material in a container, and stir the medium material through the stirring device provided on the container; or directly load the stirred culture material into the container;

(2) Sterilization and cooling: Pour steam into the container for steam sterilization, and cool after sterilization;

(3) Inoculation: Insert the strain into the container, and start the agitator on the container to stir while the strain is inserted. The technical problem to be solved by the present invention can be further realized by the following technical solutions. The above-mentioned method for inoculating edible fungi strains is characterized in that a jacket is provided outside the container, and water of a certain temperature is passed into the jacket to adjust the temperature in the container. In this way, the temperature in the container can be conveniently adjusted, and the temperature adjustment cost is low. The present invention can also use temperature adjustment methods and equipment disclosed in the prior art to adjust the temperature in the container.

In the above-mentioned inoculation method of edible fungus strains, the charging, sterilization, cooling, and inoculation of steps (1)-(3) are all carried out in a container. After the inoculation is completed, the mycelium culture can be carried out according to the method of the present invention, or the hypha culture and growth period can be carried out according to conventional techniques, until the finished edible fungus is obtained by cultivation.

The technical problem to be solved by the present invention can be further realized by the following technical solutions. The invention also discloses a method for culturing edible fungus hyphae, which is characterized in that the steps are as follows:

(2) Sterilization and cooling: Pour steam into the container for steam sterilization, and cool down after sterilization;

(3) Inoculation: Insert the strain into the container, and start the stirrer on the container for stirring while the strain is inserted. ；

(4) Cultivation of hyphae: The inoculated culture medium is cultured in the container until the hyphae growth period is completed;

(5) Load the culture device: Put the hyphae in the culture device under the required environmental conditions.

In the above technical solution, steps (4) and (5) can be replaced by the following steps (4) and (5):

(4) Loading culture equipment: Immediately after inoculation, the culture material is loaded into the culture equipment under the required environmental conditions; or, the inoculated culture material is first cultivated in the container for a period of time. Before the completion of the period, load the culture medium into the culture device under the required environmental conditions;

(5) Cultivation of hyphae: Place the culture device in the culture room for cultivation until the growth period of the hyphae is completed.

Therefore, in the method for culturing edible fungus hyphae of the present invention, after the inoculation process of step (3) is completed, the inoculated culture medium can be immediately loaded into a culture device (culture bottle, bag, or other Applicable container, the same below), let the inoculated culture medium be cultured in the culture tool for the pre-hyphae culture to the hyphae growth period (that is, the feeding period, the same below); you can also use the inoculated culture medium The material is placed in the container and cultivated for any period of time, and the culture material is loaded before the completion of the growth period of the hyphae Cultivation equipment, and then culture according to the conventional method until the growth period of the hypha is completed; the inoculated culture medium can also be placed in a container for cultivation until the growth period of the hypha is completed. After the growth period of the mycelium is completed, it is then loaded into the culture device according to the conventional method in the field to carry out the later culture (post-ripening culture period) and growth period of the mycelium, until the finished edible fungus is cultivated.

In the method of culturing mycelia in the above aspect, when cultured mycelia in a container, the container also requires a clean, temperature control, humidity, the control (0 ₂ concentration and other conventional culture conditions. Cultivation was charged The equipment should be completed in a clean environment. After the culturing equipment is installed, the hyphae should be cultured in a conventional culturing room. The culturing room needs conventional conditions such as cleanliness, temperature control, humidity control, and CO ₂ concentration. The bottling machine is carried out, and it needs to be clean when it is installed in the culture equipment. The conditions of "cleanliness, temperature control, humidity control, and CO ₂ concentration control" mentioned in the present invention refer to the inoculation and cultivation of edible fungi unless otherwise specified. Conventional air, temperature, humidity, indicators, CO ₂ concentration and other indicators needed in the process.

The technical problem to be solved by the present invention can be further realized by the following technical solutions. The above-mentioned edible fungus mycelium culture method is characterized in that a jacket is provided outside the container, and water of a certain temperature is passed into the jacket to adjust the temperature in the container. In this way, the temperature in the container can be conveniently adjusted, and the temperature adjustment cost is low. The present invention can also use the temperature adjustment methods and equipment disclosed in the prior art to adjust the temperature in the container.

The technical problem to be solved by the present invention can be further realized by the following technical solutions. The above-mentioned edible fungus mycelium culture method is characterized in that, in the hyphal culture process of step (4), the concentration of carbon dioxide in the container is adjusted by feeding clean air into the container, and the concentration of carbon dioxide in the container is adjusted while feeding clean air. , The gas in the container is replaced with clean gas.

The technical problem to be solved by the present invention can further realize the above-mentioned edible fungus mycelium culture method through the following technical solutions, which is characterized in that clean air with a certain humidity is fed into the container to adjust the culture medium in the container. humidity.

The technical problem to be solved by the present invention can be further realized by the following technical solutions. The present invention also discloses an edible fungus inoculation or mycelial culture device for realizing the above-mentioned inoculation method or mycelial culture method, which is characterized in that it includes a container with a stirring device on the container, and a Several import and export. The container is used to fill the culture material, and the stirring device is used to stir the culture material before inoculation, so that the culture material is uniform; the stirring device can also stir while inoculation Mix, so that the strains are evenly connected to the culture medium.

The technical problem to be solved by the present invention can be further realized by the following technical solutions. The above-mentioned edible fungus inoculation or mycelial culture device is characterized in that a jacket is provided outside the container, and water inlet and outlet pipes are provided on the jacket. The setting of the jacket is mainly to realize the adjustment of the temperature in the container, and the adjustment of the temperature in the container can be carried out in any process step.

The technical problem to be solved by the present invention can be further realized by the following technical solutions. The above-mentioned edible fungus inoculation or mycelial culture device is characterized in that a jacket temperature sensor is provided on the jacket.

The technical problem to be solved by the present invention can be further realized by the following technical solutions. The above-mentioned edible fungus inoculation or mycelial culture device is characterized in that the stirring device is arranged in the container, the stirring device includes a stirring shaft, and the stirring shaft is provided with a stirrer, and the stirring shaft extends out of the container and the motor and transmission Device connection. One way of setting up this kind of stirring device is that the stirring shaft and the stirrer rotate but the container does not move, or the container rotates while the stirrer does not move. The present invention has no special requirements on the stirring device. Any stirring device disclosed in the prior art that can achieve the purpose of the stirring of the present invention can be used in the present invention, and the above-mentioned stirring device is one of the preferred technical solutions.

The technical problem to be solved by the present invention can be further realized by the following technical solutions. The above-mentioned edible fungus inoculation or mycelial culture device is characterized in that the inlet and outlet provided on the container include a material outlet, a material inlet, an exhaust port and several spouts. Among them, the material outlet is used for discharging. After inoculation, the culture material or the culture material that grows mycelia needs to be taken out from the material outlet when it needs to be bottled; the material inlet can supply the culture material, solid strains, etc. into the container ; The spout can be used for clean gas, liquid bacteria, water, steam, etc. into the container; the exhaust port is for exhaust.

The technical problem to be solved by the present invention can be further realized by the following technical solutions. The above-mentioned edible fungus inoculation or mycelial culture device is characterized in that the spout is provided with a spout protection valve. The spout protection is opened when the spout is sprayed, and closed after the spout is sprayed, which can effectively prevent the culture medium from clogging the spout.

The technical problem to be solved by the present invention can be further realized by the following technical solutions. The above-mentioned edible fungus inoculation or mycelial culture device is characterized in that there is a material at the material inlet Import quick opening door; There is a material outlet quick opening door on the material outlet. The quick-opening door can adopt the conventional quick-opening setting.

The technical problem to be solved by the present invention can be further realized by the following technical solutions. The above-mentioned edible fungus inoculation or mycelial culture device is characterized in that the exhaust port is connected with a vacuum pump.

The technical problem to be solved by the present invention can be further realized by the following technical solutions. The above-mentioned edible fungus inoculation or mycelial culture device is characterized in that the material outlet is connected to a purification vehicle, and an air shower and a closed automatic bottling or bagging machine are installed in the purification vehicle. This is the preferred bottling device of the present invention. The device is small overall and easy to use. Since the culture material after inoculation or the culture material that grows mycelium needs to be taken out from the material outlet when bottling, the bottling conditions are generally clean environment (class 100) or clean environment (class 100,000). Any device or method disclosed in the prior art can be applicable to the present invention as long as it can provide the required environmental conditions for the bottling operation of the present invention.

The technical problem to be solved by the present invention can be further realized by the following technical solutions. The above-mentioned edible fungus inoculation or mycelial culture device is characterized in that the bottom of the purification vehicle is provided with movable wheels.

The technical problem to be solved by the present invention can be further realized by the following technical solutions. The above-mentioned edible fungus inoculation or mycelial culture device is characterized in that the container is also provided with a pressure sensor, a container temperature sensor, a humidity sensor and a carbon dioxide concentration sensor. In this way, various environmental parameters in the container can be conveniently controlled.

The technical problem to be solved by the present invention can be further realized by the following technical solutions. The above-mentioned edible fungus inoculation or mycelial culture device is characterized in that the container is fixedly connected to the frame.

The multifunctional edible fungus mycelium culture device of the present invention can have the following functions at the same time: one is the stirring function; the other is the sterilization function; the third is the cooling function; the fourth is the inoculation function; the fifth is it can be used as a pre-culture room.

Compared with the prior art, the inoculation method, culture method and inoculation or culture device of the present invention can be said to be a revolutionary innovation in the method and equipment of inoculation and pre-hyphae culture. Its technology has the following advantages: 1. Short feeding period, High cultivation efficiency. The method of the present invention carries out stirring while inoculating, so that The strains can be more evenly loaded into the culture medium and are not easily contaminated. The growth of the hyphae does not need to be eaten from top to bottom, but is carried out in a divergent multi-directional manner, so the hyphae can grow up quickly. , The feeding period is greatly shortened; according to the test calculation, the feeding period is shortened by about 60%, which greatly reduces the energy cost for maintaining the cultivation conditions during the cultivation of edible fungi, and at the same time shortens the cultivation period. Improved cultivation efficiency;

2. The training cost is low. The cost of training is mainly reflected in the cost of equipment and energy consumption. The multiple steps of the method of the present invention can be completed in a container, and the container itself does not need to be in a special culture environment. Therefore, it not only reduces the equipment investment, but also saves the process flow. The energy consumption cost of the conditions required by the process steps is also greatly reduced, and the production of edible fungi will not be affected.

3. The chance of infection of the strain is small. Since multiple steps of the method of the present invention can be completed in one container, there is no need to connect between equipment after each step is completed: the bacteria are directly connected to the container after sterilization, and there is no need between sterilization and inoculation. Equipment connection between steps; after inoculation, it can be cultured for a period of time and then loaded into the culture device, or after the feeding period is completed, it can be loaded into the culture device, so that the device connection between steps can not be carried out when it is most susceptible to infection; this can greatly reduce The probability of bacterial infection;

4. The device of the present invention can conveniently adjust the culture parameters in the container during the mycelial culture period, and the adjustment method is simpler and more energy-saving;

5. The device of the present invention has a simple and reasonable structure, strong operability, and low cost.

Description of the drawings

Figure 1 is a schematic diagram of an edible fungus inoculation or mycelial culture device.

Detailed ways

The following further describes the specific technical solutions of the present invention, so that those skilled in the art can further understand the present invention, without restricting the rights thereof.

Example 1. Refer to Figure 1. A method for inoculating edible fungi strains, the steps are as follows:

(1) Loading: Pack the culture medium material in a container 2, and stir the culture base material through a stirring device provided on the container 2; or directly load the stirred culture material into the container 2;

(2) Sterilization and cooling: Pour steam into the container 2 for steam sterilization, and cool after the sterilization; (3) Inoculation: The strain is connected to the container 2, and the stirring device on the container 2 is started to stir while the strain is inserted. -Example 2. In the method for inoculating edible fungi strains described in Example 1, a jacket 1 is provided outside the container 2 and water of a certain temperature is passed into the jacket 1 to adjust the temperature in the container 2.

Example 3. Refer to Figure 1. A method for cultivating edible fungus hyphae, the steps are as follows:

(1) Filling: Put the culture medium in a container 2, and stir the culture base through the stirring device provided on the container 2; or directly load the stirred culture medium into the container 2;

(2) Sterilization and cooling: Pour steam into container 2 for steam sterilization, and cool after sterilization;

(3) Inoculation: Connect the strains to container 2 and start the stirring device on container 2 for stirring at the same time as the strains are inserted;

(4) Cultivation of hyphae: The inoculated culture medium is cultured in container 2 for hyphae until the hyphae growth period is completed;

Example 4. In the edible fungus mycelium culture method described in Example 3, the container 2 is provided with a jacket 1, and water of a certain temperature is injected into the jacket 1 to adjust the temperature in the container.

Example 5. In the edible fungus mycelium culture method described in embodiment 3 or 4, in the hyphal culture process of step 4, the concentration of carbon dioxide in the container 2 is adjusted by feeding clean air into the container 2, and the concentration of carbon dioxide in the container 2 is adjusted while feeding the clean air , The gas in the container 2 is replaced with the clean gas.

Example 6. In the method for cultivating edible fungus hyphae described in embodiment 3 or 4 or 5, clean air with a certain humidity is fed into the container 2 to adjust the humidity of the culture medium in the container 2.

Example 7. Refer to Figure 1. A method for cultivating edible fungus hyphae, the steps are as follows:

(4) Load the culture device: immediately after the inoculation, load the culture medium into the culture device under the required environmental conditions; (5) Cultivating mycelium: Place the culture device in the culture room for cultivation until the growth period of the mycelium is completed. Example 8. Refer to Figure 1. A method for cultivating edible fungus hyphae, the steps are as follows:

(2) Sterilization and cooling: Steam is passed into the container 2 for steam sterilization, and the sterilization is completed and then cooled;

(3) Inoculation: Connect the strains to the container 2, and start the stirring device on the container 2 for stirring at the same time as the strains are inserted;

(4) Load the culture device: Cultivate the inoculated culture medium in the container 2 for a period of time, and then load the culture medium into the culture device under the required environmental conditions before the growth period of the mycelium is completed;

Example 9. In the edible fungus mycelium culture method described in embodiment 7 or 8, a jacket 1 is provided outside the container 2, and water of a certain temperature is injected into the jacket 1 to adjust the temperature in the container 2.

Example 10. Refer to Figure 1. An edible fungus inoculation or mycelial culture device for realizing the method described in Examples 1-9, which includes a container 2, on which a stirring device is provided, and on the container 2 is provided with several inlets and outlets.

Example 11. In the edible fungus inoculation or mycelial culture device described in embodiment 10, a jacket 1 is provided outside the container 2 and a water inlet and outlet pipeline 5 is provided on the jacket 1.

Example 12. In the edible fungus inoculation or mycelial culture device described in embodiment 10 or 11, a jacket temperature sensor 6 is provided on the jacket 1.

Example 13. In the edible fungus inoculation or mycelial culture device described in any one of embodiments 10-12, the stirring device is arranged in the container 2, and the stirring device includes a stirring shaft 4, and a stirrer 3 is provided on the stirring shaft 4, and stirring The shaft 4 extends out of the container 2 and is connected to the motor 15 and the transmission device 14.

Example 14. In the edible fungus inoculation or mycelial culture device described in any one of embodiments 10-13, the inlet and outlet provided on the container 2 include a material outlet 23, a material inlet 21, an exhaust port 22, and a number of spouts 7.

Example 15. In the edible fungus inoculation or mycelial culture device described in embodiment 14, the spout 7 is provided with a spout protection valve 8.

Example 16. In the edible fungus inoculation or mycelial culture device described in embodiment 14, at the material inlet There is a material inlet quick-opening door 11 on 21; a material outlet quick-opening door 17 on the material outlet 23. Example 17. In the edible fungus inoculation or mycelial culture device described in Embodiment 14, the exhaust port 22 is connected to the vacuum pump 13.

Example 18. In the edible fungus inoculation or mycelium culture device of embodiment 14 or 16, the material outlet 23 is connected to a purification vehicle 19, and the purification vehicle 19 is equipped with an air shower and a closed automatic bottling or bagging machine .

Example 19. In the edible fungus inoculation or mycelial culture device described in Embodiment 18, the purification vehicle 19 is provided with movable wheels 24 at the bottom.

Example 20. In the edible fungus inoculation or mycelial culture device described in any one of embodiments 1-19, the container 2 is further provided with a pressure sensor 16, a container temperature sensor 9, a humidity sensor 10, and a carbon dioxide concentration sensor 12.

Example 21. In the edible fungus inoculation or mycelial culture device described in any one of embodiments 1-20, the container 2 is fixedly connected to the frame 20.

Experimental example 1. Comparative example of liquid strain Pleurotus eryngii cultivation experiment. In this example, the cultivation experiment was carried out with the cultivation method of the prior art and the method of the present invention, and the results were compared.

Edible fungus strain: Pleurotus eryngii liquid strain, provided by Lianyungang Guosheng Biological Technology Co., Ltd. In the cost calculation, the cost of equipment and energy consumption for the production of 10,000 culture flasks (the volume of each culture flask is 1100 ml) is used to calculate, and only the process and the process and The equipment is indistinguishable, so no comparison is made during the experiment.

Experiment 1. Basic experiment of liquid strain. According to the method of the present invention.

1. Related experimental equipment and cost calculation.

(1) The edible fungus inoculation or mycelium culture device shown in Figure 1 is provided by Lianyungang Guosheng Biotechnology Co., Ltd., and its structure is as follows:

It includes a container 2 with a stirring device on the container 2 and a number of inlets and outlets on the container 2; a jacket 1 is provided outside the container 2 and a water inlet and outlet pipeline 5 is provided on the jacket 1; on the jacket 1 There is a jacketed temperature sensor 6; the stirring device is located in the container 2, the stirring device includes a stirring shaft 4, the stirring shaft 4 is provided with a stirrer 3, the stirring shaft 4 extends out of the container 2 and the motor 15 and the transmission device 14 Connection; The inlet and outlet set on the container 2 include a material outlet 23, a material inlet 21, an exhaust port 22 and a number of nozzles 7, The nozzle 7 is provided with a nozzle protection valve 8, and a material inlet quick opening door 11 is provided on the material inlet 21; a material outlet quick opening door 17 is provided on the material outlet 23; the exhaust opening 22 is connected to the vacuum pump 13; The material outlet 23 is connected to the purification vehicle 19. In the purification vehicle 19, there is an air shower and a closed automatic bottling or bagging machine. The bottom of the purification vehicle 19 is provided with moving wheels 24; and the container 2 is also provided There are a pressure sensor 16, a container temperature sensor 9, a humidity sensor 10 and a carbon dioxide concentration sensor 12; the container 2 is fixedly connected to the rack 20.

(2) Conventional cultivation room and other equipment. Provided by Lianyungang Guosheng Biological Technology Co., Ltd.

(3) Analysis of equipment cost and energy consumption cost.

Using the Pleurotus eryngii liquid strain, the total time from loading to completion of the feeding period is 15 days, of which: 1 day from loading to completion of inoculation, and 14 days after inoculation to completion of feeding period. Calculate the construction and operating costs of the required buildings and equipment as follows:

(1) The cost of equipment construction is shown in Table 1:

Table 1

(2) The cost of building construction is shown in Table 2:

Table 2

The total construction cost of buildings and equipment is 684,000 yuan. (3) The operating cost of the equipment for the production of 10,000 culture bottles for 15 days from filling to completion of the feeding period is shown in Table 3:

table 3

(4) Steam consumption cost of the sterilizer:

It consumes 1,500 kilograms of steam and is worth 255 yuan.

(5) Personnel expenditure is 1040 yuan.

The total operating cost is approximately RMB 2600.

2. The cultivation steps are as follows:

(1) Filling: Put the culture material in a container 2, and stir the culture material through the stirring device on the container 2; the stirring time is about 4 hours; while stirring, the cooling water is introduced into the jacket , To maintain the proper temperature of the culture medium in the container;

(2) Sterilization and cooling: Pour high-temperature steam into the container 2, steam sterilize the culture medium for 4 hours, cool after the sterilization, and pour cooling water into the jacket during cooling, and the cooling is completed in about 8 hours;

(3) Inoculation: Connect the Pleurotus eryngii liquid strain into the container 2 through the spout 7, and start the stirring device on the container 2 to stir at the same time as the strain is inserted; the inoculation time is about 1 hour;

(4) Cultivation of hyphae: The inoculated medium material is cultured in container 2 for hyphae until the hyphae growth period is completed, and the feed period for hyphae culture is about 14 days;

(5) Load the culture apparatus: Put the hyphae in the culture bottle through the purification vehicle;

(6) Post-ripening period: Place the culture bottle in the culture room for the post-ripening period culture of the hyphae for 10 days; Then according to the conventional growth and culture for about 20 days, the finished Pleurotus eryngii was obtained.

3. Training results.

(1) The finished product Pleurotus eryngii 1600Kg is cultivated in 10000 bottles of culture flasks;

(2) The equipment and construction cost is approximately RMB 684,000; the total operating cost is approximately RMB 26,000;

(3) A total of 15 days from loading to completion of the feeding period, 10 days after maturity, and 45 days total cultivation time;

(4) The infection rate is 0.1%.

Experiment 2: Liquid strain control experiment. According to existing technology.

1. Cultivation equipment and buildings. Provided by Lianyungang Guosheng Biological Technology Co., Ltd.

2. Related experimental equipment and cost calculation.

Based on the production of 10,000 culture flasks (1100 ml), using liquid strains, the total time from mixing to completion of the feeding period is 25 days, of which: about 1 day from mixing to inoculation, and after inoculation to completion of the feeding period 24 days. Calculate the construction cost and operating cost of the required buildings and equipment as follows:

(1) The calculation of each equipment and adult body is shown in Table 4:

Table 4

(2) The experimental buildings are shown in Table 5:

table 5

Serial number building name area cost 1 Mixing machine room 40 square meters 1000 yuan/square meter

2 Bottling machine room 350 square meters 1000 yuan / square meter

3 Inoculation room 20 square meters 1000 yuan / square meter

4 Supporting corridors and attached rooms 100 square meters 1000 yuan/square meter

5 Training room 100 square meters 1000 yuan / square meter

A total of 610 square meters 610,000 yuan

(3) The operating cost of the equipment is shown in Table 6:

Table 6

(4) Steam consumption cost of the sterilizer: 2000 kg of steam is consumed, and a total of 340 yuan is required.

(5) Personnel expenses: 3040 yuan.

Total building and equipment construction costs: 1.561 million yuan; Total operating costs: 6300 yuan. 2. The cultivation steps are as follows:

(1) Mixing: Stir the culture medium evenly according to the required humidity; it takes 4 hours;

(2) Bottling: Put the stirred medium material in a culture bottle, compact it, and punch a number of stomata on the medium material, and then press the bottle cap; it takes 2 hours;

(3) Sterilization: Transport the packed culture bottle to a sterilizer for sterilization, and cool it in a clean environment after sterilization; it takes 17 hours; (4) Inoculation: Put the bacteria into the sterilized culture bottle; it takes 2 hours;

(5) culturing mycelia: in a clean, temperature control, humidity _Ϋ were cultured under conditions of controlled concentration of C0 _2, to the culture flask covered with mycelium; eat feed mycelium culture period of 24 days;

(6) Post-ripening period: Place the culture bottle in the culture room to cultivate the hyphae during post-ripening period for 10 days; then, according to conventional growth and culture for 20 days, the finished Pleurotus eryngii is obtained.

3. Experimental results.

(1) 1552Kg of finished Pleurotus eryngii by culturing 10,000 bottles of culture flasks;

(2) The equipment and construction cost is about 1.561 million yuan; the total operating cost is about 6300 yuan;

(3) A total of 25 days from the mixing to the completion of the feeding period, the post-ripening period is 10 days, and the total cultivation time is 55 days;

(4) The infection rate is 3%.

in conclusion:

It can be seen from the results of the above comparative experiments: Compared with the control experiment, the liquid strain basic experiment has the advantages of low cost, short feeding period, short total cultivation time, low infection rate, and high yield.

Experimental example 2. Comparison of solid strain Pleurotus eryngii cultivation experiment. In this example, the cultivation experiment was carried out with the cultivation method of the prior art and the method of the present invention, and the results were compared.

Edible fungus strains: Pleurotus eryngii solid strains, provided by Lianyungang Guosheng Biological Technology Co., Ltd. In the cost calculation, the cost of equipment and energy consumption for the production of 10,000 culture flasks (the volume of each culture flask is 1100 ml) is used to calculate, and only the process and the process and The equipment is indistinguishable, so no comparison is made during the experiment.

Experiment 1. Basic experiment of solid strains. According to the method of the present invention.

1. Related experimental equipment and cost calculation.

It includes a container 2 with a stirring device on the container 2 and a number of inlets and outlets on the container 2; a jacket 1 is provided outside the container 2 and a water inlet and outlet pipeline 5 is provided on the jacket 1; and on the jacket 1 With a jacket temperature sensor 6; The stirring device is located in the container 2, the stirring device includes a stirring shaft 4, the stirring shaft 4 is provided with a stirrer 3, the stirring shaft 4 extends out of the container 2 and the motor 15 and the transmission device 14 Connection; The inlet and outlet set on the container 2 include a material outlet 23, a material inlet 21, an exhaust port 22 and a number of nozzles 7, The nozzle 7 is provided with a nozzle protection valve 8, and a material inlet quick opening door 11 is provided on the material inlet 21; a material outlet quick opening door 17 is provided on the material outlet 23; the exhaust outlet 22 is connected to the vacuum pump 13 The material outlet 23 is connected to the purification vehicle 19, in the purification vehicle 19 is equipped with an air shower and a closed automatic bottling or bagging machine, the bottom of the purification vehicle 19 is provided with moving wheels 24; container 2 also A pressure sensor 16, a container temperature sensor 9, a humidity sensor 10, and a carbon dioxide concentration sensor 12 are provided; the container 2 is fixedly connected to the rack 20.

(3) Analysis of equipment cost and energy consumption cost.

Using solid strains of Pleurotus eryngii, the total time from loading to completion of the feeding period is 19 days, of which: 1 day from loading to completion of inoculation, and 18 days after inoculation to completion of feeding period. Calculate the construction and operating costs of the required buildings and equipment as follows:

(1) See Table 7 for equipment construction costs:

Table 7

(2) The construction cost of the building is shown in Table 8:

Table 8

Serial number building name area cost

Edible fungus inoculation or mycelium

1 50 square meters 1000 yuan / square meter

Room for cultivation equipment

2 Supporting corridors and annex rooms 100 square meters 1000 yuan / square meter

3 Cultivation room 100 square meters 1000 yuan / square meter A total of 250 square meters X 1000 yuan = 250,000 yuan, the total construction cost of buildings and equipment is 906,000 yuan.

(3) The operating cost of the equipment for 19 days from loading to completion of the feeding period is shown in Table 9-Table 9

(4) Steam consumption cost of the sterilizer:

It consumes 1,500 kilograms of steam and is worth 255 yuan.

(5) Personnel expenditure is 1,200 yuan.

The total operating cost is approximately RMB 3,500.

2. The cultivation steps are as follows:

(1) Filling: Put the culture material in a container 2, and stir the culture material through the stirring device on the container 2; the stirring time is about 4 hours; while stirring, the cooling water is passed into the jacket , To maintain the proper temperature of the culture medium in the container;

(3) Inoculation: Connect the Pleurotus eryngii liquid strain into the container 2 through the spout 7, and start the stirring device on the container 2 to stir at the same time the strain is inserted; the inoculation time is about 1 hour;

(4) Bottling: Put the inoculated culture medium into the culture bottle through the purification truck; (5) Cultivating mycelium: place the culture bottle in the culture room for mycelial culture until the hyphal growth period is completed, and the feed period for mycelial culture is about 18 days;

(6) Post-ripening period: Place the culture bottle in the culture room to culture the hyphae during post-ripening period for 10 days; and then cultivate for about 20 days to obtain the finished Pleurotus eryngii.

3. Training results.

(1) 1584Kg of finished Pleurotus eryngii by culturing 10,000 bottles of culture flasks;

(2) The cost of equipment and construction is approximately RMB 906,000; the total operating cost is approximately RMB 3,500;

(3) A total of 19 days from loading to completion of eating period, 10 days after maturity, and 49 days total cultivation time;

(4) The infection rate is 1%.

Experiment 2: Solid strain control experiment. According to existing technology.

2. Related experimental equipment and cost calculation.

Based on the production of 10,000 culture flasks (1100 ml), using solid strains, the total time from mixing to the completion of the feeding period is 29 days, of which: about 1 day from mixing to inoculation, and after inoculation to completion of the feeding period 28 days. Calculate the construction cost and operating cost of the required buildings and equipment as follows:

(1) See Table 10 for the calculation of each equipment and adult body:

Table 10

(2) The experimental buildings are shown in Table 11:

Table 11

(3) Equipment operating costs are shown in Table 12:

Table 12

(5) Personnel expenditure: 3200 yuan.

Total building and equipment construction costs: 1.461 million yuan; total operating costs: 0.69 million yuan. 2. The cultivation steps are as follows:

(2) Bottling: Put the stirred medium material in a culture flask, compact it, and place it on the medium material Punch several air holes, and then press the bottle cap; it takes 2 hours;

(3) Sterilization: transport the packed culture bottle to a sterilizer for sterilization, and cool it in a clean environment after sterilization; it takes 17 hours;

(4) Inoculation: Put the bacteria into the sterilized culture bottle; it takes 2 hours;

(5) Cultivation of hyphae: culture under the conditions of cleanness, temperature control, humidity control, and CO ₂ concentration until the culture flask is full of hyphae; the feed period for hyphae culture is 28 days;

3. Experimental results.

(2) The equipment and construction cost is approximately RMB 1.461 million; the total operating cost is approximately RMB 0.69 million;

(3) A total of 29 days from the mixing to the completion of the feeding period, the after-maturing period is 10 days, and the total cultivation time is 59 days;

(4) The infection rate is 3%.

in conclusion:

It can be seen from the results of the above comparative experiments: Compared with the control experiment, the solid strain basic experiment has the advantages of low cost, short feeding period, short total cultivation time, low infection rate, and high yield.

Claims

Claims, a method for inoculating edible fungi strains, characterized in that the steps are as follows: (1) Filling: The culture material is filled in a container (2), and the culture medium is placed on the container (2). The stirring device stirs the culture medium; or directly puts the stirred culture medium into the container (2); (2) Sterilization and cooling: Steam the container (2) for steam sterilization, and the sterilization is over After cooling; (3) Inoculation: Insert the strain into the container (2), and start the stirring device on the container (2) for stirring while the strain is inserted. The method for inoculating edible fungi strains according to claim 1, wherein a jacket (1) is provided outside the container (2), and water of a certain temperature is passed into the jacket (1). Adjust the temperature in the container (2). 1. A method for cultivating edible fungus mycelium, characterized in that the steps are as follows: (1) Loading: The culture material is packed in a container (2), and the mixing device is arranged on the container (2). Stir the medium material; or directly put the stirred medium material into the container (2); (2) Sterilization and cooling: Steam the container (2) for steam sterilization, and cool after the sterilization is completed; (3) Inoculation: Connect the strains to the container (2), and start the stirring device on the container (2) for stirring while the strains are inserted; The hyphae are cultured in the container (2) until the hyphae growth period is completed; (5) Put into the culture device: Put the hyphae into the culture device under the required environmental conditions. The edible fungus mycelium culture method according to claim 3, characterized in that a jacket (1) is provided outside the container (2), and water of a certain temperature is injected into the jacket (1) to adjust the container内的温度。 The temperature within. The edible fungus mycelium culture method according to claim 3 or 4, characterized in that, in the mycelium culture process of step (4), the container (2) is adjusted by feeding clean air into the container (2) The carbon dioxide concentration in the container (2) is replaced with the clean gas while the clean air is fed into it. The edible fungus mycelium culture method according to claim 3 or 4, characterized in that clean air with a certain humidity is fed into the container (2) to adjust the humidity of the culture medium in the container (2). A method for culturing edible fungus hyphae, characterized in that the steps are as follows:

(1) Loading: Put the culture material in a container (2), and stir the culture material through the stirring device provided on the container (2); or directly load the stirred culture material into the container ( 2) Within;

(2) Sterilization and cooling: Pour steam into the container (2) for steam sterilization, and cool down after sterilization;

(3) Inoculation: Connect the strains to the container (2), and start the stirring device on the container (2) for stirring at the same time as the strains are inserted;

(4) Load the culture equipment: Immediately after the inoculation, load the culture medium into the culture equipment under the required environmental conditions; or, first cultivate the inoculated culture material in the container (2) for a period of time. Before the silk growth period is completed, load the culture medium into the culture device under the required environmental conditions;

(5) Cultivating mycelium: Place the culture device in the culture room for cultivation until the growth period of the mycelium is completed. 3. The method for cultivating edible fungus hyphae according to claim 7, wherein the container

(2) A jacket (1) is provided outside, and water of a certain temperature is injected into the jacket (1) to adjust the temperature in the container (2).

2. An edible fungus inoculation or mycelial culture device for realizing the method of claim 1 or 3 or 7, characterized in that it comprises a container (2), a stirring device is arranged on the container (2), and the container ( 2) There are several entrances and exits on it.

0. The edible fungus inoculation or mycelial culture device according to claim 9, characterized in that the container (2) is provided with a jacket (1), and the jacket (1) is provided with a water inlet and outlet pipe ( 5).

1. The edible fungus inoculation or mycelial culture device according to claim 10, characterized in that a jacket temperature sensor (6) is provided on the jacket (1).

2. The edible fungus inoculation or mycelial culture device according to claim 9 or 10, characterized in that the stirring device is arranged in the container (2), and the stirring device comprises a stirring shaft (4) and a stirring shaft

(4) is equipped with a stirrer (3), the stirring shaft (4) extends out of the container (2) and the motor (15) and The transmission (14) is connected.

The edible fungus inoculation or mycelial culture device according to claim 9 or 10, characterized in that the inlet and outlet provided on the container (2) include a material outlet (23), a material inlet (21), and an exhaust port ( 22) And several spouts (7).

The edible fungus inoculation or mycelial culture device according to claim 13, characterized in that the spout (7) is provided with a spout protection valve (8).

The edible fungus inoculation or mycelial culture device according to claim 13, characterized in that a material inlet quick opening door (11) is provided on the material inlet (21); a material outlet quick opening door (11) is provided on the material outlet (23) Open the door (17).

The edible fungus inoculation or mycelial culture device according to claim 13, wherein the exhaust port (22) is connected to a vacuum pump (13).

The edible fungus inoculation or mycelial culture device according to claim 13, characterized in that the material outlet (23) is connected to a purification vehicle (19), and the purification vehicle (19) is provided with an air shower and Closed automatic bottling or bagging machine.

The edible fungus inoculation or mycelial culture device according to claim 14, characterized in that the bottom of the purification vehicle (19) is provided with movable wheels (24).

The edible fungus inoculation or mycelial culture device according to claim 9 or 10, characterized in that the container (2) is further provided with a pressure sensor (16), a container temperature sensor (9), and a humidity sensor (10) And carbon dioxide concentration sensor (12).

The edible fungus inoculation or mycelial culture device according to claim 9 or 10, characterized in that the container (2) is fixedly connected to the frame (20).