CN108476867A - The sterilizing cooling inoculation Integration Equipment of culture medium of edible fungus - Google Patents

The sterilizing cooling inoculation Integration Equipment of culture medium of edible fungus Download PDF

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Publication number
CN108476867A
CN108476867A CN201810574288.1A CN201810574288A CN108476867A CN 108476867 A CN108476867 A CN 108476867A CN 201810574288 A CN201810574288 A CN 201810574288A CN 108476867 A CN108476867 A CN 108476867A
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CN
China
Prior art keywords
cooling
culture medium
inoculation
machine
edible fungus
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CN201810574288.1A
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Chinese (zh)
Inventor
徐寿海
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Lianyungang Guoxin Edible Fungus Of Complete Sets Of Equipment Co Ltd
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Lianyungang Guoxin Edible Fungus Of Complete Sets Of Equipment Co Ltd
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Priority to CN201810574288.1A priority Critical patent/CN108476867A/en
Publication of CN108476867A publication Critical patent/CN108476867A/en
Pending legal-status Critical Current

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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • A01G18/30Accessories for use before inoculation of spawn, e.g. sterilisers
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • A01G18/50Inoculation of spawn
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • A01G18/60Cultivation rooms; Equipment therefor
    • A01G18/69Arrangements for managing the environment, e.g. sprinklers

Abstract

A kind of sterilizing cooling inoculation Integration Equipment of culture medium of edible fungus, the equipment includes sterilizer and cooling inoculation machine, it is provided with the interior room for carrying out high-temperature sterilization to culture medium of edible fungus in sterilizer, vacuum orifice, interior room steam inlet and the inlet port and outlet port passed in and out convenient for culture medium of edible fungus are provided in interior room;It is provided with and is connected to interior room discharge port and by the feeding mouth of inlet valve control opening and closing on the cooling inoculation machine, the feed opening by outlet valve control opening and closing is additionally provided on cooling inoculation machine, it is provided with and is additionally provided in cooling inoculation machine convenient for the temperature in cooling inoculation machine to be reduced to the temperature cooling body of inoculation temperature for will be to cooling and stirring device that culture medium is stirred on cooling inoculation machine.The equipment reasonable design, Commercial cultivation are efficient, and an equipment can be completed to the sterilizing of culture medium of edible fungus, cool down, be inoculated with operation, substantially reduce the incubation time of edible mushroom.

Description

The sterilizing cooling inoculation Integration Equipment of culture medium of edible fungus
Technical field
The present invention relates to technical field of edible fungi production, the sterilizing cooling inoculation one of especially a kind of culture medium of edible fungus Makeup is standby and the sterilizing cooling inoculation method of edible mushroom is carried out using the equipment.
Background technology
Manufacturing process of edible fungi in the prior art:By the culture medium being stirred in bacterium bottle (bag), by bottle(Bag)After put Enter sterilizing in sterilizing cabinet, sterilizing carries out cultural hypha after terminating after cooling, inoculation, since strain is seeded in training in access Support in primary surface or inoculation hole, thus the growth of mycelia be all from surface gradually down growth or centered on inoculation hole around Diffusion growth waits for that mycelia covers with bacterium bottle(Bag)It is sent into fertility room fruiting afterwards.
Defect of the existing technology:
1, cultivation cycle is long:According to kind difference, the cultivation cycle of edible mushroom also can different from, by taking mushroom as an example, mycelia is long It completely needs 34 days or so;
2, the stirring during such Edible Fungi, bottling(Bag), sterilizing, cooling, inoculation, culture, the links such as fruiting be all It is individually carried out by corresponding equipment, production cycle length, inefficiency, labor intensity are big, and production cost is high.
Invention content
The technical problem to be solved by the present invention is in view of the deficiencies of the prior art, provide a kind of reasonable design, operation side Just, can effectively shorten inoculation after the cultural hypha time culture medium of edible fungus sterilizing cooling inoculation Integration Equipment.
There is provided use the sterilizing of above-mentioned culture medium of edible fungus to cool down for another technical problem to be solved by this invention The sterilizing cooling inoculation method of the progress edible mushroom of inoculation integral equipment.
The technical problem to be solved by the present invention is to what is realized by technical solution below.The present invention is a kind of edible The sterilizing cooling inoculation Integration Equipment of bacterium culture medium, the equipment include sterilizer and cooling inoculation machine, are provided in sterilizer For the interior room to culture medium of edible fungus progress high-temperature sterilization, it is provided with vacuum orifice, interior room steam inlet in interior room and is convenient for The inlet port and outlet port of culture medium of edible fungus disengaging;It is provided on the cooling inoculation machine and is connected to and leads to interior room discharge port The feeding mouth of inlet valve control opening and closing is crossed, the feed opening by outlet valve control opening and closing is additionally provided on cooling inoculation machine, it is cooling It is provided on inoculation device convenient for the temperature in cooling inoculation machine to be reduced to the temperature cooling body of inoculation temperature, cooling inoculation machine It is inside additionally provided with for will be to cooling and stirring device that culture medium is stirred.
The technical problems to be solved by the invention can also be further realized by technical solution below, for above The sterilizing cooling inoculation Integration Equipment of the culture medium of edible fungus is additionally provided in the sterilizer abundant convenient for culture medium Heated heating and stirring device.
The technical problems to be solved by the invention can also be further realized by technical solution below, for above The sterilizing cooling inoculation Integration Equipment of the culture medium of edible fungus is additionally provided on the cooling inoculation machine convenient for that will eat Bacterium strain sprays into the inoculation nozzle in cooling inoculation machine.
The technical problems to be solved by the invention can also be further realized by technical solution below, for above The sterilizing cooling inoculation Integration Equipment of the culture medium of edible fungus is connected with blanking at the feed opening of the cooling inoculation machine It manages, is horizontally installed on tremie pipe convenient for carrying out the inoculation nozzle of injection inoculation to culture medium when culture medium discharges.
The technical problems to be solved by the invention can also be further realized by technical solution below, for above The sterilizing cooling inoculation Integration Equipment of the culture medium of edible fungus is both provided with just on the sterilizer and cooling inoculation machine In the spiral discharge device of conveying culture medium outward.
The technical problems to be solved by the invention can also be further realized by technical solution below, for above The sterilizing cooling inoculation Integration Equipment of the culture medium of edible fungus, the temperature cooling body include being sleeved on cooling inoculation outside Cooling sandwith layer on machine and/or the vacuum fast-cooling system being connect with cooling inoculation machine by heat exchanger.
The technical problems to be solved by the invention can also be further realized by technical solution below, for above The sterilizing cooling inoculation Integration Equipment of the culture medium of edible fungus, the sterilizer and cooling inoculation machine are both provided with and are convenient for The temperature sensor that edible fungus species, culture medium temperature are detected.
The technical problems to be solved by the invention can also be further realized by technical solution below, and one kind is edible The sterilizing cooling inoculation method of bacterium, the sterilizing cooling inoculation Integration Equipment of the culture medium of edible fungus described in this method use, Steps are as follows:
(1)Culture medium of edible fungus is added to the interior interior of sterilizer by feed inlet, and steam is passed through by inside interior and is carried out Interior room is preheated;
(2)Vacuumize process is carried out to the interior room of sterilizer, after vacuumizing, steaming is passed through by the inside interior in interior room steam inlet Vapour carries out heating sterilizing to culture medium, while carrying out sterilization processing into steam into cooling inoculation machine;
(3)After sterilizing, opens inlet valve and deliver into the culture medium in sterilizer in cooling inoculation machine;Charging terminates Afterwards, inlet valve is closed, opening temperature cooling body is quickly cooled down the culture medium in cooling inoculation machine, makes culture medium Temperature is down to inoculation temperature;
(4)Culture medium after cooling is inoculated with, inoculation uses any one of following two methods:
Method one is to be sprayed into liquid edible bacterium strain in cooling inoculation machine by the inoculator on cooling inoculation machine, is opened simultaneously Cooling and stirring device, is inoculated with while stirring;
Culture medium conveying after cooling is packed into holder by method second is that unlatching outlet valve, and cultivator is packed into culture medium Have in overweight journey and culture medium is inoculated with;
(5)For step(4)In be inoculated with according to method one, then after inoculation, open outlet valve, food will be inoculated with It is packed into holder with the culture medium conveying of bacterium strain, then holder is sent into culturing room and carries out cultural hypha;It is right In step(4)In be inoculated with according to method two, then after inoculation, directly by equipped with culture medium holder be sent into Cultural hypha is carried out in culturing room.
The technical problems to be solved by the invention can also be further realized by technical solution below, for above The sterilizing cooling inoculation method of the edible mushroom, the inoculation temperature are 22 DEG C -28 DEG C.
Compared with prior art, the present invention carries out high-temperature sterilization by sterilizer to culture medium, utilizes cooling inoculation machine pair Culture medium carries out cooling inoculation, not only realizes the continuous operation of the sterilizing cooling inoculation of edible mushroom, but also to the height of culture medium Temperature sterilizing is operated with cooling inoculation using split, is carried out at the same time convenient for the two, is substantially increased operating efficiency;Secondly, temperature The stand-by period of operation is greatly saved to being cooled down rapidly to cooling inoculation machine in cooling body.Equipment reasonable design, the life Produce that utilization ratio is high, an equipment can be completed to the sterilizing of culture medium of edible fungus, cool down, be inoculated with operation, substantially reduce food With the incubation time of bacterium.
Description of the drawings
Fig. 1 is a kind of structural schematic diagram of the present invention;
Fig. 2 is structural schematic diagram of the present invention without heating and stirring device;
Fig. 3 is structural schematic diagram of the present invention without vacuum fast-cooling system;
Fig. 4 is the structural schematic diagram of the invention with heating and stirring device.
Specific implementation mode
In order to make the object, technical scheme and advantages of the embodiment of the invention clearer, right below in conjunction with attached drawing of the present invention Technical solution in the embodiment of the present invention is clearly and completely described, it is clear that described embodiment is a part of the invention Embodiment, instead of all the embodiments.Based on the embodiments of the present invention, those of ordinary skill in the art are not making wound The every other embodiment obtained under the premise of the property made labour, shall fall within the protection scope of the present invention.
With reference to figure 1, embodiment 1, a kind of sterilizing cooling inoculation Integration Equipment of culture medium of edible fungus, which includes going out Bacterium machine 1 and cooling inoculation machine 2 are provided with the interior room 6 for carrying out high-temperature sterilization to culture medium of edible fungus, interior room 6 in sterilizer 1 On be provided with vacuum orifice 9,6 steam inlet of interior room and the feed inlet 8 and discharge port passed in and out convenient for culture medium of edible fungus;It is described cold But it is provided with and is connected to 6 discharge port of interior room and by the feeding mouth of the control opening and closing of inlet valve 7, cooling inoculation machine 2 on inoculation device 2 On be additionally provided with feed opening by the control opening and closing of outlet valve 12, be provided on cooling inoculation machine 2 convenient for will be in cooling inoculation machine 2 Temperature be reduced to the temperature cooling body of inoculation temperature, be additionally provided in cooling inoculation machine 2 for that will stir culture medium The cooling and stirring device 11 mixed.Sterilizer 1 is used to carry out high-temperature sterilization to culture medium, and feed inlet 8 is sealed by quick operating valve, convenient Charging, closing;Cooling inoculation machine 2 is used to receive the culture medium in the sterilizer 1 after sterilizing, then using temperature cooling body into Row cools, convenient for being passed through edible fungus species, by cooling and stirring device 11 by edible bacterial vaccine inoculation to culture medium, so After be transported to the enterprising luggage bag of sack filling machine, or when exporting culture medium outward, be inoculated with;Cooling and stirring device 11 includes The agitating shaft being horizontally installed in cooling inoculation machine 2 is provided with propeller on agitating shaft, abundant convenient for being carried out to culture medium The side of stirring, cooling inoculation machine 2 is provided with driving motor, and driving motor is connected by chain or chain wheel driving mechanism with agitating shaft It connects.
It is additionally provided in the sterilizer 1 convenient for the fully heated heating and stirring device 10 of culture medium.In the sterilizer 1 It is additionally provided with convenient for the fully heated heating and stirring device 10 of culture medium.Heating and stirring device 10 includes being horizontally installed on sterilizer Agitating shaft in 1 is provided with double helix agitating paddle on agitating shaft, convenient for being sufficiently stirred to culture medium, the side of sterilizer 1 It is provided with driving motor, driving motor passes through chain or chain wheel driving mechanism and stirring axis connection.
It is additionally provided on the cooling inoculation machine 2 convenient for edible fungus species to be sprayed into the inoculation nozzle in cooling inoculation machine 2 3, convenient for being inoculated with to culture medium in cooling inoculation machine 2, be conducive to the more abundant of inoculation.
It is connected with tremie pipe at the feed opening of the cooling inoculation machine 2, is horizontally installed on tremie pipe convenient in culture medium The inoculation nozzle 3 for carrying out injection inoculation when discharging to culture medium is conducive to convenient for being inoculated with when exporting culture medium outward Improve inoculation efficiency.
It is both provided on the sterilizer 1 and cooling inoculation machine 2 convenient for the spiral discharge device 15 of conveying culture medium outward. Spiral discharge device 15 is provided with conducive to the delivery efficiency of culture medium is improved, and spiral discharge device 15 includes discharging auger and use In the discharging machine of driving discharging auger rotation.
The temperature cooling body include the cooling sandwith layer being sleeved on outside on cooling inoculation machine 2 and/or by heat exchanger 5 with it is cold But the vacuum fast-cooling system 4 that inoculation device 2 connects is provided with cooling water at the top of cooling sandwith layer and is passed through mouth 13, the bottom of cooling sandwith layer Portion is provided with cooling water outlet 14.Temperature cooling body is cooling sandwith layer either vacuum fast-cooling system 4 or cooling sandwith layer And vacuum cooling system.Vacuum fast-cooling system 4 is the prior art, vacuum rapid cooling machine, vacuum cooling system etc. can also be used existing Cooling system replaces;By the heat exchange of heat exchanger 5, the temperature in cooling inoculation machine 2 is on the one hand effectively reduced, is on the one hand also avoided Pollution is generated to cooling inoculation machine 2.
The sterilizer 1 and cooling inoculation machine 2 are both provided with to be detected edible fungus species, culture medium temperature Temperature sensor.The sterilizer 1 and cooling inoculation machine 2 are both provided with to examine edible fungus species, culture medium temperature The temperature sensor of survey.The temperature sensor of setting has several, is respectively used to monitoring interior room 6, cooling inoculation machine 2 and culture medium Temperature.
A kind of sterilizing cooling inoculation method of edible mushroom, this method are cold using the sterilizing of above-described culture medium of edible fungus But inoculation integral is equipped, and its step are as follows:
(1)Culture medium of edible fungus is added by feed inlet 8 in the interior room 6 of sterilizer 1, and by being passed through steam into interior room 6 Interior room 6 is preheated;
(2)Vacuumize process is carried out to the interior room 6 of sterilizer 1, after vacuumizing, is passed through into interior room 6 by 6 steam inlet of interior room Steam carries out heating sterilizing to culture medium, while carrying out sterilization processing into steam into cooling inoculation machine 2;
(3)After sterilizing, opens inlet valve 7 and deliver into the culture medium in sterilizer 1 in cooling inoculation machine 2;Charging knot Shu Hou, closes inlet valve 7, and opening temperature cooling body is quickly cooled down the culture medium in cooling inoculation machine 2, makes culture The temperature of base is down to inoculation temperature;
(4)Culture medium after cooling is inoculated with, inoculation uses any one of following two methods:
Method one is to be sprayed into liquid edible bacterium strain in cooling inoculation machine 2 by the inoculator on cooling inoculation machine 2, is opened simultaneously Cooling and stirring device 11 is opened, is inoculated with while stirring;
Culture medium conveying after cooling is packed into holder second is that unlatching outlet valve 12, is packed into and cultivates in culture medium by method Culture medium is inoculated in the overweight journey of utensil;
(5)For step(4)In be inoculated with according to method one, then after inoculation, open outlet valve 12, will be inoculated with The culture medium conveying of edible fungus species is packed into holder, and then holder is sent into culturing room and carries out cultural hypha; For step(4)In be inoculated with according to method two, then after inoculation, directly the holder equipped with culture medium is sent Enter progress cultural hypha in culturing room.
The inoculation temperature is 22 DEG C -28 DEG C.Preferably, the inoculation temperature is 25 DEG C.
Step(3)In, after charging, continuing step(4)While, culture can be added into sterilizer 1 again Base carries out disinfecting action, convenient for after inoculation, the culture medium after sterilizing being continued to be fed into cooling inoculation machine 2, to realize Continuous operation effectively improves operating efficiency.
Embodiment 2, a kind of sterilizing cooling inoculation Integration Equipment of culture medium of edible fungus, it is characterised in that:The equipment packet Sterilizer 1 and cooling inoculation machine 2 are included, the interior room 6 for carrying out high-temperature sterilization to culture medium of edible fungus is provided in sterilizer 1, Vacuum orifice 9,6 steam inlet of interior room and the feed inlet 8 and discharge port passed in and out convenient for culture medium of edible fungus are provided in interior room 6; The feeding mouth for being connected to 6 discharge port of interior room and being opened and closed by the control of inlet valve 7 is provided on the cooling inoculation machine 2, cooling connects It is additionally provided with the feed opening by the control opening and closing of outlet valve 12 on kind machine 2, is provided with and is convenient for cooling inoculation on cooling inoculation machine 2 Temperature in machine 2 is reduced to the temperature cooling body of inoculation temperature, be additionally provided in cooling inoculation machine 2 for will to culture medium into The cooling and stirring device 11 of row stirring.
It is additionally provided on the cooling inoculation machine 2 convenient for edible fungus species to be sprayed into the inoculation nozzle in cooling inoculation machine 2 3。
The temperature cooling body includes the cooling sandwith layer being sleeved on outside on cooling inoculation machine 2, the top setting of cooling sandwith layer There is cooling water to be passed through mouth 13, the bottom of cooling sandwith layer is provided with cooling water outlet 14.
It is both provided on the sterilizer 1 and cooling inoculation machine 2 convenient for the spiral discharge device 15 of conveying culture medium outward.
It is not provided with agitating device in sterilizer 1, it is then right convenient for improving efficiency for being inoculated with the culture medium being stirred Cooling sandwith layer is passed through cooling water and cools down to carrying out the culture medium in cooling inoculation machine 2, and inoculation nozzle 3 is recycled to be connect in cooling Culture medium is inoculated in kind.
With reference to figure 2, embodiment 3, a kind of sterilizing cooling inoculation Integration Equipment of culture medium of edible fungus, it is characterised in that: The equipment includes sterilizer 1 and cooling inoculation machine 2, is provided in sterilizer 1 for carrying out high-temperature sterilization to culture medium of edible fungus Interior room 6, be provided with vacuum orifice 9,6 steam inlet of interior room and 8 and of feed inlet passed in and out convenient for culture medium of edible fungus in interior room 6 Discharge port;It is provided with and is connected to 6 discharge port of interior room and by the pan feeding of the control opening and closing of inlet valve 7 on the cooling inoculation machine 2 Mouthful, it is additionally provided with the feed opening by the control opening and closing of outlet valve 12 on cooling inoculation machine 2, is provided with and is convenient on cooling inoculation machine 2 Temperature in cooling inoculation machine 2 is reduced to the temperature cooling body of inoculation temperature, is additionally provided in cooling inoculation machine 2 for inciting somebody to action To the cooling and stirring device 11 that culture medium is stirred,
It is connected with tremie pipe at the feed opening of the cooling inoculation machine 2, is horizontally installed on tremie pipe convenient for discharging in culture medium When to culture medium carry out injection inoculation inoculation nozzle 3.
The temperature cooling body includes the vacuum fast-cooling system 4 being connect with cooling inoculation machine 2 by heat exchanger 5.
It is both provided on the sterilizer 1 and cooling inoculation machine 2 convenient for the spiral discharge device 15 of conveying culture medium outward.
It is not provided with agitating device in sterilizer 1, it is then sharp convenient for improving efficiency for being inoculated with the culture medium being stirred Fast cooling is carried out to entering the culture medium in cooling inoculation machine 2 with vacuum fast-cooling system 4, inoculation nozzle 3 is recycled to cultivate Base is inoculated with when being conveyed outward from cooling inoculation machine 2.
With reference to figure 3, embodiment 4, a kind of sterilizing cooling inoculation Integration Equipment of culture medium of edible fungus, it is characterised in that: The equipment includes sterilizer 1 and cooling inoculation machine 2, is provided in sterilizer 1 for carrying out high-temperature sterilization to culture medium of edible fungus Interior room 6, be provided with vacuum orifice 9,6 steam inlet of interior room and 8 and of feed inlet passed in and out convenient for culture medium of edible fungus in interior room 6 Discharge port;It is provided with and is connected to 6 discharge port of interior room and by the pan feeding of the control opening and closing of inlet valve 7 on the cooling inoculation machine 2 Mouthful, it is additionally provided with the feed opening by the control opening and closing of outlet valve 12 on cooling inoculation machine 2, is provided with and is convenient on cooling inoculation machine 2 Temperature in cooling inoculation machine 2 is reduced to the temperature cooling body of inoculation temperature, is additionally provided in cooling inoculation machine 2 for inciting somebody to action The cooling and stirring device 11 that culture medium is stirred.
It is additionally provided in the sterilizer 1 convenient for the fully heated heating and stirring device 10 of culture medium.
It is additionally provided on the cooling inoculation machine 2 convenient for edible fungus species to be sprayed into the inoculation nozzle in cooling inoculation machine 2 3。
The temperature cooling body includes the cooling sandwith layer being sleeved on outside on cooling inoculation machine 2, the top setting of cooling sandwith layer There is cooling water to be passed through mouth 13, the bottom of cooling sandwith layer is provided with cooling water outlet 14.
It is provided with agitating device in sterilizer 1, can carry out high-temperature sterilization while stirring to the culture medium of entrance, while can be with By the uniform of culture medium stirring, it is convenient for subsequent inoculations strain, is then passed through cooling water to entering cooling inoculation machine 2 to cooling sandwith layer Interior culture medium carries out fast cooling, and inoculation nozzle 3 is recycled to be inoculated with to culture medium in cooling inoculation machine 2.
With reference to figure 4, embodiment 5, a kind of sterilizing cooling inoculation Integration Equipment of culture medium of edible fungus, it is characterised in that: The equipment includes sterilizer 1 and cooling inoculation machine 2, is provided in sterilizer 1 for carrying out high-temperature sterilization to culture medium of edible fungus Interior room 6, be provided with vacuum orifice 9,6 steam inlet of interior room and 8 and of feed inlet passed in and out convenient for culture medium of edible fungus in interior room 6 Discharge port;It is provided with and is connected to 6 discharge port of interior room and by the pan feeding of the control opening and closing of inlet valve 7 on the cooling inoculation machine 2 Mouthful, it is additionally provided with the feed opening by the control opening and closing of outlet valve 12 on cooling inoculation machine 2, is provided with and is convenient on cooling inoculation machine 2 Temperature in cooling inoculation machine 2 is reduced to the temperature cooling body of inoculation temperature, is additionally provided in cooling inoculation machine 2 for inciting somebody to action The cooling and stirring device 11 that culture medium is stirred.
It is additionally provided in the sterilizer 1 convenient for the fully heated heating and stirring device 10 of culture medium.
It is connected with tremie pipe at the feed opening of the cooling inoculation machine 2, is horizontally installed on tremie pipe convenient in culture medium The inoculation nozzle 3 of injection inoculation is carried out when discharging to culture medium.
The temperature cooling body includes the vacuum fast-cooling system 4 being connect with cooling inoculation machine 2 by heat exchanger 5.
It is provided with agitating device in sterilizer 1, can carry out high-temperature sterilization while stirring to the culture medium of entrance, while can be with By the uniform of culture medium stirring, it is convenient for subsequent inoculations strain, then utilizes vacuum fast-cooling system 4 to entering in cooling inoculation machine 2 Culture medium carry out fast cooling, recycle inoculation nozzle 3 culture medium from cooling inoculation machine 2 outward convey when be inoculated with.
Compared with prior art, there are following advantages by the application:
1, cultivation cycle is short, and culture medium is stirred while inoculation, and strain is made uniformly to be mixed with culture medium;Mycelia is being planted Realize that multiple spot is sprouted when training culture in utensil, by taking mushroom as an example, mycelia can cover with bacteria stick within 14 days, saved closely compared with proterotype 58% time;
2, Commercial cultivation rate is high, is fed directly to cooling inoculation machine after medium sterilization, and sterilizer can feed sterilizing again, Cooling inoculation machine carries out cooling inoculation to culture medium simultaneously, realizes continuous operation;
Toxigenic capacity is saved, stirring, sterilizing, cooling, the inoculation of the application are completed in sterilizer and cooling inoculation machine, no Workshop, equipment, personnel cost are only reduced, technological process is also simplified.

Claims (9)

1. a kind of sterilizing cooling inoculation Integration Equipment of culture medium of edible fungus, it is characterised in that:The equipment include sterilizer and Cooling inoculation machine is provided with the interior room for carrying out high-temperature sterilization to culture medium of edible fungus in sterilizer, pumping is provided in interior room Vacuum port, interior room steam inlet and the inlet port and outlet port convenient for culture medium of edible fungus disengaging;It is set on the cooling inoculation machine It is equipped with the feeding mouth for being connected to interior room discharge port and being opened and closed by inlet valve control, is additionally provided on cooling inoculation machine by going out Expect the feed opening of valve control opening and closing, is provided on cooling inoculation machine convenient for the temperature in cooling inoculation machine is reduced to inoculation temperature Temperature cooling body, be additionally provided in cooling inoculation machine for will be to cooling and stirring device that culture medium is stirred.
2. the sterilizing cooling inoculation Integration Equipment of culture medium of edible fungus according to claim 1, it is characterised in that:It is described It is additionally provided in sterilizer convenient for the fully heated heating and stirring device of culture medium.
3. the sterilizing cooling inoculation Integration Equipment of culture medium of edible fungus according to claim 1 or 2, it is characterised in that: It is additionally provided on the cooling inoculation machine convenient for edible fungus species to be sprayed into the inoculation nozzle in cooling inoculation machine.
4. the sterilizing cooling inoculation Integration Equipment of culture medium of edible fungus according to claim 1 or 2, it is characterised in that: It is connected with tremie pipe at the feed opening of the cooling inoculation machine, is horizontally installed with and is convenient for when culture medium discharges to training on tremie pipe Foster base carries out the inoculation nozzle of injection inoculation.
5. the sterilizing cooling inoculation Integration Equipment of culture medium of edible fungus according to claim 1, it is characterised in that:It is described It is both provided on sterilizer and cooling inoculation machine convenient for the spiral discharge device of conveying culture medium outward.
6. the sterilizing cooling inoculation Integration Equipment of culture medium of edible fungus according to claim 1, it is characterised in that:It is described Temperature cooling body includes the cooling sandwith layer being sleeved on outside on cooling inoculation machine and/or is connect with cooling inoculation machine by heat exchanger Vacuum fast-cooling system.
7. the sterilizing cooling inoculation Integration Equipment of culture medium of edible fungus according to claim 1, it is characterised in that:It is described Sterilizer and cooling inoculation machine are both provided with the temperature sensor convenient for being detected to edible fungus species, culture medium temperature.
8. a kind of sterilizing cooling inoculation method of edible mushroom, it is characterised in that:This method uses claim 1-7 any one institute The sterilizing cooling inoculation Integration Equipment for the culture medium of edible fungus stated, its step are as follows:
(1)Culture medium of edible fungus is added to the interior interior of sterilizer by feed inlet, and steam is passed through by inside interior and is carried out Interior room is preheated;
(2)Vacuumize process is carried out to the interior room of sterilizer, after vacuumizing, steaming is passed through by the inside interior in interior room steam inlet Vapour carries out heating sterilizing to culture medium, while carrying out sterilization processing into steam into cooling inoculation machine;
(3)After sterilizing, opens inlet valve and deliver into the culture medium in sterilizer in cooling inoculation machine;Charging terminates Afterwards, inlet valve is closed, opening temperature cooling body is quickly cooled down the culture medium in cooling inoculation machine, makes culture medium Temperature is down to inoculation temperature;
(4)Culture medium after cooling is inoculated with, inoculation uses any one of following two methods:
Method one is to be sprayed into liquid edible bacterium strain in cooling inoculation machine by the inoculator on cooling inoculation machine, is opened simultaneously Cooling and stirring device, is inoculated with while stirring;
Culture medium conveying after cooling is packed into holder by method second is that unlatching outlet valve, and cultivator is packed into culture medium Have in overweight journey and culture medium is inoculated with;
(5)For step(4)In be inoculated with according to method one, then after inoculation, open outlet valve, food will be inoculated with It is packed into holder with the culture medium conveying of bacterium strain, then holder is sent into culturing room and carries out cultural hypha;It is right In step(4)In be inoculated with according to method two, then after inoculation, directly by equipped with culture medium holder be sent into Cultural hypha is carried out in culturing room.
9. the sterilizing cooling inoculation method of edible mushroom according to claim 8, it is characterised in that:The inoculation temperature is 22 ℃-28℃。
CN201810574288.1A 2018-06-06 2018-06-06 The sterilizing cooling inoculation Integration Equipment of culture medium of edible fungus Pending CN108476867A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113940236A (en) * 2021-10-27 2022-01-18 浙江百兴食品有限公司 High-temperature high-pressure sterilization inoculation pot for edible fungus culture medium

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113940236A (en) * 2021-10-27 2022-01-18 浙江百兴食品有限公司 High-temperature high-pressure sterilization inoculation pot for edible fungus culture medium

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