WO2011006285A1 - Méthode d’inoculation d’un champignon comestible survivant dans les graines, méthode de culture du mycélium et dispositif associé - Google Patents

Méthode d’inoculation d’un champignon comestible survivant dans les graines, méthode de culture du mycélium et dispositif associé Download PDF

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Publication number
WO2011006285A1
WO2011006285A1 PCT/CN2009/001518 CN2009001518W WO2011006285A1 WO 2011006285 A1 WO2011006285 A1 WO 2011006285A1 CN 2009001518 W CN2009001518 W CN 2009001518W WO 2011006285 A1 WO2011006285 A1 WO 2011006285A1
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Prior art keywords
container
culture
inoculation
edible fungus
stirring
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PCT/CN2009/001518
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English (en)
Chinese (zh)
Inventor
徐寿海
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Xu Shouhai
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00—Cultivation of mushrooms
    • A01G18/20—Culture media, e.g. compost
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00—Cultivation of mushrooms
    • A01G18/30—Accessories for use before inoculation of spawn, e.g. sterilisers
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00—Cultivation of mushrooms
    • A01G18/40—Cultivation of spawn
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00—Cultivation of mushrooms
    • A01G18/50—Inoculation of spawn
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00—Cultivation of mushrooms
    • A01G18/60—Cultivation rooms; Equipment therefor
    • A01G18/64—Cultivation containers; Lids therefor

Definitions

  • the present invention relates to the cultivation of edible fungi, especially the inoculation method of edible fungi; the present invention also relates to a method for cultivating seed-feeding fungus hyphae; the present invention also relates to a device for realizing the above-mentioned edible fungus inoculation or hyphae culture.
  • the cultivation of edible fungi can be divided into cultivation period and growth period.
  • the cultivation period is divided into the pre-hyphae culture and the hyphal post-mature cultivation period; the growth period is divided into the early growth and the late growth.
  • the early growth is to cultivate the edible mushroom stalk, and the later growth is from the stalk stage to the edible fungus. Reproductive maturity.
  • the culture method of inoculation and pre-hyphae culture in the prior art is divided into the following 5 steps:
  • Bottling Put the stirred medium material in a culture bottle (bag), compact it, and punch a number of pores on the medium material, and then press the bottle cap;
  • Sterilization transport the packed culture bottle (bag) to a sterilizer for sterilization, and cool it in a clean environment after sterilization;
  • Cultivation of hyphae culture under the conditions of cleanness, temperature control, humidity control, and CO 2 concentration until the culture bottle is full of hyphae; because the strains are inoculated at the mouth of the culture bottle when they are connected, Therefore, the growth of mycelium gradually grows from the mouth of the bottle to the bottom of the bottle to cover the entire culture bottle (also called food); the period from the beginning of the growth of the mycelium to fullness in this step is also called the food period.
  • the feeding period is long and the cultivation efficiency is low. According to different strains, the cultivation period of edible fungi will be different.
  • the cultivation period of Pleurotus eryngii is about 35 days. Among them: the culture period of the hyphae at the early stage is about 25 days, and the culture period of the hyphae at the later maturity is about 10 days. It can be seen from this that, in the prior art, the early mycelial culture of edible fungi takes a lot of time, while the first four steps (mixing, bottling, sterilization, and inoculation) of the early mycelial culture are time-consuming. Generally it is about 1 day, basically negligible, because Almost all of the 25 days required are spent in the feeding period of step 5.
  • the cost of training is mainly reflected in two aspects: one is the cost of equipment; the other is the cost of energy consumption to maintain the cultivation environment.
  • the steps of mixing, bottling, sterilization and inoculation all require certain equipment and a demanding environment.
  • the cultivation environment during the feeding period needs to be clean, temperature controlled, humidity controlled, and CO 2 controlled.
  • To maintain the cultivation environment requires a lot of equipment and energy, so how to shorten the feeding period It is a major problem that the existing technology needs to solve in order to save energy and cost.
  • the technical problem to be solved by the present invention is to address the shortcomings of the prior art and provide a method for inoculating edible fungi strains that requires relatively simple equipment and can shorten the mycelial culture time after inoculation.
  • Another technical problem to be solved by the present invention is to provide two methods for cultivating edible fungus mycelium that are short in time and can greatly save cultivation energy and cost.
  • Another technical problem to be solved by the present invention is to provide a device for realizing the above-mentioned edible fungus seed inoculation method or edible fungus mycelium culture method.
  • the method and device of the invention are suitable for the inoculation and cultivation of liquid strains and solid strains.
  • the technical problem to be solved by the present invention is achieved through the following technical solutions.
  • the present invention is a method for inoculating edible fungi strains, and its characteristic is that the steps are as follows:
  • the technical problem to be solved by the present invention can be further realized by the following technical solutions.
  • the above-mentioned method for inoculating edible fungi strains is characterized in that a jacket is provided outside the container, and water of a certain temperature is passed into the jacket to adjust the temperature in the container. In this way, the temperature in the container can be conveniently adjusted, and the temperature adjustment cost is low.
  • the present invention can also use temperature adjustment methods and equipment disclosed in the prior art to adjust the temperature in the container.
  • the charging, sterilization, cooling, and inoculation of steps (1)-(3) are all carried out in a container.
  • the mycelium culture can be carried out according to the method of the present invention, or the hypha culture and growth period can be carried out according to conventional techniques, until the finished edible fungus is obtained by cultivation.
  • the technical problem to be solved by the present invention can be further realized by the following technical solutions.
  • the invention also discloses a method for culturing edible fungus hyphae, which is characterized in that the steps are as follows:
  • steps (4) and (5) can be replaced by the following steps (4) and (5):
  • Cultivation of hyphae Place the culture device in the culture room for cultivation until the growth period of the hyphae is completed.
  • the inoculated culture medium can be immediately loaded into a culture device (culture bottle, bag, or other Applicable container, the same below), let the inoculated culture medium be cultured in the culture tool for the pre-hyphae culture to the hyphae growth period (that is, the feeding period, the same below); you can also use the inoculated culture medium
  • the material is placed in the container and cultivated for any period of time, and the culture material is loaded before the completion of the growth period of the hyphae Cultivation equipment, and then culture according to the conventional method until the growth period of the hypha is completed; the inoculated culture medium can also be placed in a container for cultivation until the growth period of the hypha is completed.
  • the growth period of the mycelium is completed, it is then loaded into the culture device according to the conventional method in the field to carry out the later culture (post-ripening culture period
  • the container when cultured mycelia in a container, the container also requires a clean, temperature control, humidity, the control (0 2 concentration and other conventional culture conditions. Cultivation was charged
  • the equipment should be completed in a clean environment. After the culturing equipment is installed, the hyphae should be cultured in a conventional culturing room.
  • the culturing room needs conventional conditions such as cleanliness, temperature control, humidity control, and CO 2 concentration.
  • the bottling machine is carried out, and it needs to be clean when it is installed in the culture equipment.
  • the conditions of "cleanliness, temperature control, humidity control, and CO 2 concentration control" mentioned in the present invention refer to the inoculation and cultivation of edible fungi unless otherwise specified. Conventional air, temperature, humidity, indicators, CO 2 concentration and other indicators needed in the process.
  • the technical problem to be solved by the present invention can be further realized by the following technical solutions.
  • the above-mentioned edible fungus mycelium culture method is characterized in that a jacket is provided outside the container, and water of a certain temperature is passed into the jacket to adjust the temperature in the container. In this way, the temperature in the container can be conveniently adjusted, and the temperature adjustment cost is low.
  • the present invention can also use the temperature adjustment methods and equipment disclosed in the prior art to adjust the temperature in the container.
  • the technical problem to be solved by the present invention can be further realized by the following technical solutions.
  • the above-mentioned edible fungus mycelium culture method is characterized in that, in the hyphal culture process of step (4), the concentration of carbon dioxide in the container is adjusted by feeding clean air into the container, and the concentration of carbon dioxide in the container is adjusted while feeding clean air. , The gas in the container is replaced with clean gas.
  • the technical problem to be solved by the present invention can further realize the above-mentioned edible fungus mycelium culture method through the following technical solutions, which is characterized in that clean air with a certain humidity is fed into the container to adjust the culture medium in the container. humidity.
  • the technical problem to be solved by the present invention can be further realized by the following technical solutions.
  • the present invention also discloses an edible fungus inoculation or mycelial culture device for realizing the above-mentioned inoculation method or mycelial culture method, which is characterized in that it includes a container with a stirring device on the container, and a Several import and export.
  • the container is used to fill the culture material, and the stirring device is used to stir the culture material before inoculation, so that the culture material is uniform; the stirring device can also stir while inoculation Mix, so that the strains are evenly connected to the culture medium.
  • the technical problem to be solved by the present invention can be further realized by the following technical solutions.
  • the above-mentioned edible fungus inoculation or mycelial culture device is characterized in that a jacket is provided outside the container, and water inlet and outlet pipes are provided on the jacket.
  • the setting of the jacket is mainly to realize the adjustment of the temperature in the container, and the adjustment of the temperature in the container can be carried out in any process step.
  • the technical problem to be solved by the present invention can be further realized by the following technical solutions.
  • the above-mentioned edible fungus inoculation or mycelial culture device is characterized in that a jacket temperature sensor is provided on the jacket.
  • the technical problem to be solved by the present invention can be further realized by the following technical solutions.
  • the above-mentioned edible fungus inoculation or mycelial culture device is characterized in that the stirring device is arranged in the container, the stirring device includes a stirring shaft, and the stirring shaft is provided with a stirrer, and the stirring shaft extends out of the container and the motor and transmission Device connection.
  • One way of setting up this kind of stirring device is that the stirring shaft and the stirrer rotate but the container does not move, or the container rotates while the stirrer does not move.
  • the present invention has no special requirements on the stirring device. Any stirring device disclosed in the prior art that can achieve the purpose of the stirring of the present invention can be used in the present invention, and the above-mentioned stirring device is one of the preferred technical solutions.
  • the technical problem to be solved by the present invention can be further realized by the following technical solutions.
  • the above-mentioned edible fungus inoculation or mycelial culture device is characterized in that the inlet and outlet provided on the container include a material outlet, a material inlet, an exhaust port and several spouts.
  • the material outlet is used for discharging. After inoculation, the culture material or the culture material that grows mycelia needs to be taken out from the material outlet when it needs to be bottled; the material inlet can supply the culture material, solid strains, etc. into the container ;
  • the spout can be used for clean gas, liquid bacteria, water, steam, etc. into the container; the exhaust port is for exhaust.
  • the technical problem to be solved by the present invention can be further realized by the following technical solutions.
  • the above-mentioned edible fungus inoculation or mycelial culture device is characterized in that the spout is provided with a spout protection valve. The spout protection is opened when the spout is sprayed, and closed after the spout is sprayed, which can effectively prevent the culture medium from clogging the spout.
  • the technical problem to be solved by the present invention can be further realized by the following technical solutions.
  • the above-mentioned edible fungus inoculation or mycelial culture device is characterized in that there is a material at the material inlet Import quick opening door; There is a material outlet quick opening door on the material outlet.
  • the quick-opening door can adopt the conventional quick-opening setting.
  • the technical problem to be solved by the present invention can be further realized by the following technical solutions.
  • the above-mentioned edible fungus inoculation or mycelial culture device is characterized in that the exhaust port is connected with a vacuum pump.
  • the technical problem to be solved by the present invention can be further realized by the following technical solutions.
  • the above-mentioned edible fungus inoculation or mycelial culture device is characterized in that the material outlet is connected to a purification vehicle, and an air shower and a closed automatic bottling or bagging machine are installed in the purification vehicle.
  • This is the preferred bottling device of the present invention.
  • the device is small overall and easy to use. Since the culture material after inoculation or the culture material that grows mycelium needs to be taken out from the material outlet when bottling, the bottling conditions are generally clean environment (class 100) or clean environment (class 100,000). Any device or method disclosed in the prior art can be applicable to the present invention as long as it can provide the required environmental conditions for the bottling operation of the present invention.
  • the technical problem to be solved by the present invention can be further realized by the following technical solutions.
  • the above-mentioned edible fungus inoculation or mycelial culture device is characterized in that the bottom of the purification vehicle is provided with movable wheels.
  • the technical problem to be solved by the present invention can be further realized by the following technical solutions.
  • the above-mentioned edible fungus inoculation or mycelial culture device is characterized in that the container is also provided with a pressure sensor, a container temperature sensor, a humidity sensor and a carbon dioxide concentration sensor. In this way, various environmental parameters in the container can be conveniently controlled.
  • the technical problem to be solved by the present invention can be further realized by the following technical solutions.
  • the above-mentioned edible fungus inoculation or mycelial culture device is characterized in that the container is fixedly connected to the frame.
  • the multifunctional edible fungus mycelium culture device of the present invention can have the following functions at the same time: one is the stirring function; the other is the sterilization function; the third is the cooling function; the fourth is the inoculation function; the fifth is it can be used as a pre-culture room.
  • the inoculation method, culture method and inoculation or culture device of the present invention can be said to be a revolutionary innovation in the method and equipment of inoculation and pre-hyphae culture.
  • Its technology has the following advantages: 1. Short feeding period, High cultivation efficiency.
  • the method of the present invention carries out stirring while inoculating, so that The strains can be more evenly loaded into the culture medium and are not easily contaminated.
  • the growth of the hyphae does not need to be eaten from top to bottom, but is carried out in a divergent multi-directional manner, so the hyphae can grow up quickly.
  • the feeding period is greatly shortened; according to the test calculation, the feeding period is shortened by about 60%, which greatly reduces the energy cost for maintaining the cultivation conditions during the cultivation of edible fungi, and at the same time shortens the cultivation period. Improved cultivation efficiency;
  • the training cost is low.
  • the cost of training is mainly reflected in the cost of equipment and energy consumption.
  • the multiple steps of the method of the present invention can be completed in a container, and the container itself does not need to be in a special culture environment. Therefore, it not only reduces the equipment investment, but also saves the process flow.
  • the energy consumption cost of the conditions required by the process steps is also greatly reduced, and the production of edible fungi will not be affected.
  • the device of the present invention can conveniently adjust the culture parameters in the container during the mycelial culture period, and the adjustment method is simpler and more energy-saving;
  • the device of the present invention has a simple and reasonable structure, strong operability, and low cost.
  • Figure 1 is a schematic diagram of an edible fungus inoculation or mycelial culture device.
  • Example 1 Refer to Figure 1. A method for inoculating edible fungi strains, the steps are as follows:
  • Example 2 Sterilization and cooling: Pour steam into the container 2 for steam sterilization, and cool after the sterilization; (3) Inoculation: The strain is connected to the container 2, and the stirring device on the container 2 is started to stir while the strain is inserted. -Example 2.
  • a jacket 1 is provided outside the container 2 and water of a certain temperature is passed into the jacket 1 to adjust the temperature in the container 2.
  • Example 3 Refer to Figure 1. A method for cultivating edible fungus hyphae, the steps are as follows:
  • Example 4 In the edible fungus mycelium culture method described in Example 3, the container 2 is provided with a jacket 1, and water of a certain temperature is injected into the jacket 1 to adjust the temperature in the container.
  • Example 5 In the edible fungus mycelium culture method described in embodiment 3 or 4, in the hyphal culture process of step 4, the concentration of carbon dioxide in the container 2 is adjusted by feeding clean air into the container 2, and the concentration of carbon dioxide in the container 2 is adjusted while feeding the clean air , The gas in the container 2 is replaced with the clean gas.
  • Example 6 In the method for cultivating edible fungus hyphae described in embodiment 3 or 4 or 5, clean air with a certain humidity is fed into the container 2 to adjust the humidity of the culture medium in the container 2.
  • Example 7 Refer to Figure 1. A method for cultivating edible fungus hyphae, the steps are as follows:
  • Example 8 Refer to Figure 1. A method for cultivating edible fungus hyphae, the steps are as follows:
  • Cultivation of hyphae Place the culture device in the culture room for cultivation until the growth period of the hyphae is completed.
  • Example 9 In the edible fungus mycelium culture method described in embodiment 7 or 8, a jacket 1 is provided outside the container 2, and water of a certain temperature is injected into the jacket 1 to adjust the temperature in the container 2.
  • Example 10 Refer to Figure 1. An edible fungus inoculation or mycelial culture device for realizing the method described in Examples 1-9, which includes a container 2, on which a stirring device is provided, and on the container 2 is provided with several inlets and outlets.
  • Example 11 In the edible fungus inoculation or mycelial culture device described in embodiment 10, a jacket 1 is provided outside the container 2 and a water inlet and outlet pipeline 5 is provided on the jacket 1.
  • Example 12 In the edible fungus inoculation or mycelial culture device described in embodiment 10 or 11, a jacket temperature sensor 6 is provided on the jacket 1.
  • Example 13 In the edible fungus inoculation or mycelial culture device described in any one of embodiments 10-12, the stirring device is arranged in the container 2, and the stirring device includes a stirring shaft 4, and a stirrer 3 is provided on the stirring shaft 4, and stirring The shaft 4 extends out of the container 2 and is connected to the motor 15 and the transmission device 14.
  • Example 14 In the edible fungus inoculation or mycelial culture device described in any one of embodiments 10-13, the inlet and outlet provided on the container 2 include a material outlet 23, a material inlet 21, an exhaust port 22, and a number of spouts 7.
  • Example 15 In the edible fungus inoculation or mycelial culture device described in embodiment 14, the spout 7 is provided with a spout protection valve 8.
  • Example 16 In the edible fungus inoculation or mycelial culture device described in embodiment 14, at the material inlet There is a material inlet quick-opening door 11 on 21; a material outlet quick-opening door 17 on the material outlet 23.
  • Example 17 In the edible fungus inoculation or mycelial culture device described in Embodiment 14, the exhaust port 22 is connected to the vacuum pump 13.
  • Example 18 In the edible fungus inoculation or mycelium culture device of embodiment 14 or 16, the material outlet 23 is connected to a purification vehicle 19, and the purification vehicle 19 is equipped with an air shower and a closed automatic bottling or bagging machine .
  • Example 19 In the edible fungus inoculation or mycelial culture device described in Embodiment 18, the purification vehicle 19 is provided with movable wheels 24 at the bottom.
  • Example 20 In the edible fungus inoculation or mycelial culture device described in any one of embodiments 1-19, the container 2 is further provided with a pressure sensor 16, a container temperature sensor 9, a humidity sensor 10, and a carbon dioxide concentration sensor 12.
  • Example 21 In the edible fungus inoculation or mycelial culture device described in any one of embodiments 1-20, the container 2 is fixedly connected to the frame 20.
  • Experimental example 1 Comparative example of liquid strain Pleurotus eryngii cultivation experiment.
  • the cultivation experiment was carried out with the cultivation method of the prior art and the method of the present invention, and the results were compared.
  • Edible fungus strain Pleurotus eryngii liquid strain, provided by Lianyungang Guosheng Biological Technology Co., Ltd.
  • the cost of equipment and energy consumption for the production of 10,000 culture flasks (the volume of each culture flask is 1100 ml) is used to calculate, and only the process and the process and The equipment is indistinguishable, so no comparison is made during the experiment.
  • the edible fungus inoculation or mycelium culture device shown in Figure 1 is provided by Lianyungang Guosheng Biotechnology Co., Ltd., and its structure is as follows:
  • the inlet and outlet set on the container 2 include a material outlet 23, a material inlet 21, an exhaust port 22 and a number of nozzles 7,
  • the nozzle 7 is provided with a nozzle protection valve 8, and a material inlet quick opening door 11 is provided on the material inlet 21; a material outlet quick opening door 17 is provided on the material outlet 23; the exhaust opening 22 is connected to the vacuum pump 13;
  • the material outlet 23 is connected to the purification vehicle 19.
  • the purification vehicle 19 there is an air shower and a closed automatic bottling or bagging machine.
  • the bottom of the purification vehicle 19 is provided with moving wheels 24; and the container 2 is also provided There are a pressure sensor 16, a container temperature sensor 9, a humidity sensor 10 and a carbon dioxide concentration sensor 12; the container 2 is fixedly connected to the rack 20.
  • the total time from loading to completion of the feeding period is 15 days, of which: 1 day from loading to completion of inoculation, and 14 days after inoculation to completion of feeding period.
  • the total operating cost is approximately RMB 2600.
  • Post-ripening period Place the culture bottle in the culture room for the post-ripening period culture of the hyphae for 10 days; Then according to the conventional growth and culture for about 20 days, the finished Pleurotus eryngii was obtained.
  • Pleurotus eryngii 1600Kg is cultivated in 10000 bottles of culture flasks;
  • the infection rate is 0.1%.
  • the total time from mixing to completion of the feeding period is 25 days, of which: about 1 day from mixing to inoculation, and after inoculation to completion of the feeding period 24 days.
  • Serial number building name area cost 1 Mixing machine room 40 square meters 1000 yuan/square meter
  • Bottling machine room 350 square meters 1000 yuan / square meter
  • Bottling Put the stirred medium material in a culture bottle, compact it, and punch a number of stomata on the medium material, and then press the bottle cap; it takes 2 hours;
  • Sterilization Transport the packed culture bottle to a sterilizer for sterilization, and cool it in a clean environment after sterilization; it takes 17 hours;
  • Inoculation Put the bacteria into the sterilized culture bottle; it takes 2 hours;
  • Post-ripening period Place the culture bottle in the culture room to cultivate the hyphae during post-ripening period for 10 days; then, according to conventional growth and culture for 20 days, the finished Pleurotus eryngii is obtained.
  • the equipment and construction cost is about 1.561 million yuan; the total operating cost is about 6300 yuan;
  • the infection rate is 3%.
  • the liquid strain basic experiment has the advantages of low cost, short feeding period, short total cultivation time, low infection rate, and high yield.
  • Experimental example 2 Comparison of solid strain Pleurotus eryngii cultivation experiment.
  • the cultivation experiment was carried out with the cultivation method of the prior art and the method of the present invention, and the results were compared.
  • Edible fungus strains Pleurotus eryngii solid strains, provided by Lianyungang Guosheng Biological Technology Co., Ltd.
  • the cost of equipment and energy consumption for the production of 10,000 culture flasks (the volume of each culture flask is 1100 ml) is used to calculate, and only the process and the process and The equipment is indistinguishable, so no comparison is made during the experiment.
  • the edible fungus inoculation or mycelium culture device shown in Figure 1 is provided by Lianyungang Guosheng Biotechnology Co., Ltd., and its structure is as follows:
  • the stirring device includes a container 2 with a stirring device on the container 2 and a number of inlets and outlets on the container 2; a jacket 1 is provided outside the container 2 and a water inlet and outlet pipeline 5 is provided on the jacket 1; and on the jacket 1 With a jacket temperature sensor 6;
  • the stirring device is located in the container 2, the stirring device includes a stirring shaft 4, the stirring shaft 4 is provided with a stirrer 3, the stirring shaft 4 extends out of the container 2 and the motor 15 and the transmission device 14 Connection;
  • the inlet and outlet set on the container 2 include a material outlet 23, a material inlet 21, an exhaust port 22 and a number of nozzles 7,
  • the nozzle 7 is provided with a nozzle protection valve 8, and a material inlet quick opening door 11 is provided on the material inlet 21; a material outlet quick opening door 17 is provided on the material outlet 23; the exhaust outlet 22 is connected to the vacuum pump 13
  • the material outlet 23 is connected to the purification vehicle 19, in the purification vehicle 19 is equipped with an air shower and a
  • the total time from loading to completion of the feeding period is 19 days, of which: 1 day from loading to completion of inoculation, and 18 days after inoculation to completion of feeding period.
  • the total operating cost is approximately RMB 3,500.
  • Bottling Put the inoculated culture medium into the culture bottle through the purification truck; (5) Cultivating mycelium: place the culture bottle in the culture room for mycelial culture until the hyphal growth period is completed, and the feed period for mycelial culture is about 18 days;
  • Post-ripening period Place the culture bottle in the culture room to culture the hyphae during post-ripening period for 10 days; and then cultivate for about 20 days to obtain the finished Pleurotus eryngii.
  • the infection rate is 1%.
  • the total time from mixing to the completion of the feeding period is 29 days, of which: about 1 day from mixing to inoculation, and after inoculation to completion of the feeding period 28 days.
  • Sterilization transport the packed culture bottle to a sterilizer for sterilization, and cool it in a clean environment after sterilization; it takes 17 hours;
  • Post-ripening period Place the culture bottle in the culture room to cultivate the hyphae during post-ripening period for 10 days; then, according to conventional growth and culture for 20 days, the finished Pleurotus eryngii is obtained.
  • the infection rate is 3%.
  • the solid strain basic experiment has the advantages of low cost, short feeding period, short total cultivation time, low infection rate, and high yield.

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  • Mushroom Cultivation (AREA)

Abstract

Cette invention concerne une méthode d’inoculation d’un champignon comestible survivant dans les graines, ladite méthode consistant à : charger un milieu de culture dans un conteneur (2), à brasser ce milieu de culture au moyen d’un agitateur prévu dans le conteneur, à induire de la vapeur dans le conteneur pour une stérilisation à la vapeur, à refroidir après stérilisation, et à inoculer le champignon attaquant les graines dans le conteneur, tout en activant l’agitateur dans le conteneur. Une méthode de culture du mycélium d’un champignon comestible survivant dans les graines consiste à inoculer le champignon selon la méthode décrite ci-dessus, puis à cultiver le mycélium. Un dispositif d’inoculation du champignon ou un dispositif de culture permettant de mettre en œuvre l’inoculation et la méthode de culture décrites ci-dessus comprend un conteneur, un agitateur et quelques ports d’entrée et ports de sortie prévus dans le conteneur. Cette méthode et ce dispositif peuvent raccourcir le délai du processus et réduire le coût de la culture du champignon comestible.
PCT/CN2009/001518 2009-07-13 2009-12-29 Méthode d’inoculation d’un champignon comestible survivant dans les graines, méthode de culture du mycélium et dispositif associé WO2011006285A1 (fr)

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CN2009101823965A CN101822168B (zh) 2009-07-13 2009-07-13 食用菌接种或菌丝培养装置
CN200910182396.5 2009-07-13

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WO2011006285A1 true WO2011006285A1 (fr) 2011-01-20

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CN111406573A (zh) * 2020-03-16 2020-07-14 黄山市徽珍食品有限公司 一种食用菌综合工厂化循环生产方法
CN111713338A (zh) * 2020-06-29 2020-09-29 广西禾美生态农业股份有限公司 一种自动化食用菌固体接种系统

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CN106232798B (zh) * 2014-02-21 2019-06-07 生命科技股份有限公司 用于培养基再水合的系统、方法和设备
CN106105786A (zh) * 2016-08-30 2016-11-16 武汉开明智城科技有限公司 食用菌接种装置

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CN111406573A (zh) * 2020-03-16 2020-07-14 黄山市徽珍食品有限公司 一种食用菌综合工厂化循环生产方法
CN111406573B (zh) * 2020-03-16 2021-08-17 黄山市徽珍食品有限公司 一种食用菌综合工厂化循环生产方法
CN111713338A (zh) * 2020-06-29 2020-09-29 广西禾美生态农业股份有限公司 一种自动化食用菌固体接种系统
CN111713338B (zh) * 2020-06-29 2023-01-03 广西禾美生态农业股份有限公司 一种自动化食用菌固体接种系统

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