CN105961453B - A kind of vegetalitas culicifuge, preparation and its application of repellent Culex quinquefasciatus - Google Patents
A kind of vegetalitas culicifuge, preparation and its application of repellent Culex quinquefasciatus Download PDFInfo
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- CN105961453B CN105961453B CN201610361191.3A CN201610361191A CN105961453B CN 105961453 B CN105961453 B CN 105961453B CN 201610361191 A CN201610361191 A CN 201610361191A CN 105961453 B CN105961453 B CN 105961453B
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- Prior art keywords
- essential oil
- culicifuge
- vegetalitas
- repellent
- culex quinquefasciatus
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/36—Rutaceae [Rue family], e.g. lime, orange, lemon, corktree or pricklyash
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Agronomy & Crop Science (AREA)
- Biotechnology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Mycology (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Botany (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention relates to a kind of vegetalitas culicifuge, preparation and its application of repellent Culex quinquefasciatus, the chief active object of the culicifuge is：The composition for touching essential oil, arborvitae essential oil and Lantana camara essential oil containing one, the mass parts content of each component is one to touch 10 ~ 30 parts of 40 ~ 70 parts of essential oil, 15 ~ 30 parts of arborvitae essential oil and Lantana camara essential oil in the composition.Preparation method is made of to be uniformly mixed each raw material, emulsify, homogeneous.The vegetalitas repellant of the present invention has significant repellent effect to Culex quinquefasciatus indoors, and to safety of human and livestock's nonhazardous, is not polluted to environment.
Description
Technical field
The present invention relates to a kind of vegetalitas culicifuge of repellent Culex quinquefasciatus and its applications, belong to botanical pesticide technology neck
Domain.
Background technology
Culex quinquefasciatus（Culex quinquefasciatus）It is distributed in south China each province and city, Jiangsu that north and south has a common boundary,
The provinces such as Zhejiang, Anhui, Hubei, Shaanxi are also distributed, generally movable indoors, are indoor most important mosquitoes of sucking blood.Except harassing and wrecking
Outside the normal life of the mankind, at the same be also a kind of important viral vector, it can propagate a variety of diseases, as bancroftosis,
Mosquitos matchmaker's infectious disease such as dengue fever.
The main Metadelphene of mosquito component in the culicifuge being commonly used（DEET）, pyrethroid etc., these chemistry
Although repellant effect is good, efficiency time is long, and existing to human body toxic side effect, to environment has the drawbacks such as pollution.And it plants
The culicifuge of physical property has the characteristics that high-efficiency low-toxicity or nontoxic, environmentally friendly, plants essential oil complex chemical composition, to mosquito
Mechanism of action is unique, and often therefore same plants essential oil filters out to causing the repellent effect significant difference of different mosquitoes
Tired culex has the plants essential oil of notable repellent activity, develops the vegetalitas repellant of safety and environmental protection, to protecting human health
And its environment of existence has a very important significance.
Invention content
The object of the present invention is to provide a kind of vegetalitas culicifuges that side effect non-toxic to humans is environmentally safe, are used for
Repellent Culex quinquefasciatus has significant repellent effect.
The technical scheme is that：A kind of vegetalitas culicifuge of repellent Culex quinquefasciatus, the chief active of the culicifuge
Object is：The composition for touching essential oil, arborvitae essential oil and Lantana camara essential oil containing one, the mass parts content of each component in the composition
10 ~ 30 parts of 40 ~ 70 parts of essential oil, 15 ~ 30 parts of arborvitae essential oil and Lantana camara essential oil are touched for one.
Described one touch essential oil, arborvitae essential oil and Lantana camara essential oil be by steam distillation respectively from one touch spiceleaf,
It extracts and obtains in arborvitae branches and leaves and the wounded in the battle branches and leaves of Lantana camara.
Further, the composition further includes solvent, and solvent accounts for the 5 ~ 10% of the composition total weight.
In a specific embodiment, which is creme, and the composition accounts for the mass percentage content of the creme
It is 60 ~ 85%.Mosquito-dispelling cream further includes emulsifier 1~4%, moisturizer 1~8%, moisturizing agent 10~20%, counter-stimulus 0.1~1%, pH
Conditioning agent 0.1~0.5%, preservative 0.01~0.05% and odorant 0.1~0.5%, remaining is distilled water, adds up to 100%, to account for
The mass percent meter of the creme.
In a specific embodiment, which is spray, and the composition accounts for the mass percent of the spray
Content is 45 ~ 65%.Spray further include emulsifier 1~5%, moisturizer 1~8%, moisturizing agent 1~4%, counter-stimulus 0.1~1%,
PH adjusting agent 0.1~0.6%, preservative 0.01~0.1% and odorant 0.1~0.5%, remaining is distilled water, adds up to 100%, with
Account for the mass percent meter of the spray.
The emulsifier is one kind in polyethenoxy ether class, carboxylate, sulfate, polyoxypropylene ethers and sulfonate
Or more, moisturizer be polyalcohol, hyaluronic acid and amino acid in it is one or more of, the moisturizing agent be isomery dodecane,
One or more of in higher alcohol, vegetable and animals oils and Synthetic Oil, the counter-stimulus is α-bisabolol, the pH adjusting agent
For triethanolamine or citric acid.
The preparation method of above-mentioned vegetalitas culicifuge is made of to be uniformly mixed each raw material, emulsify, homogeneous.
The vegetalitas repellant of the present invention, touches the composition that essential oil, arborvitae essential oil and Lantana camara essential oil form by one, matches
Enter auxiliary agent and mosquito-dispelling cream, Mosquito-repellant spray be made, indoors to Culex quinquefasciatus have significant repellent effect, and to safety of human and livestock without
It poisons, environment is not polluted.Up to now it combines and is made there is not yet touching essential oil, arborvitae essential oil and Lantana camara essential oil by one
Vegetalitas culicifuge.
Specific implementation mode
The present invention vegetalitas culicifuge chief active object be：Essential oil, arborvitae essential oil and Lantana camara essential oil are touched containing one
Composition（Following " vegetalitas mosquito repellent compositions "）, the mass parts content of each component is one to touch essential oil in the composition
10 ~ 30 parts of 40 ~ 70 parts, 15 ~ 30 parts of arborvitae essential oil and Lantana camara essential oil.When preparation with absolute ethyl alcohol respectively to one touch essential oil,
Arborvitae essential oil and the dissolving of Lantana camara essential oil, then mix to obtain the final product, wherein absolute ethyl alcohol accounts for the 5 ~ 10% of mosquito repellent composition weight.
Described one touches essential oil by Rutaceae（Rutaceae）One touches perfume (or spice)（Zanthoxylum beecheyanum）Ye Jing
Steam distillation and obtain.The arborvitae essential oil is by Cupressaceae（Cupressaceae）Arborvitae（Thuja sutchuenensis）'s
Branches and leaves steam distillation and obtain.The Lantana camara essential oil is by Verenaceae（Verbenaceae）Plant Lantana camara
（Lantana camara）Wounded in the battle branches and leaves steam distillation and obtain.The steam distillation is steamed using conventional total water
It evaporates.
As needed, vegetalitas culicifuge can be made to the forms such as creme or spray.
In a specific embodiment, mosquito-dispelling cream includes following components（Following mass percents with mosquito-dispelling cream generally
Benchmark）：Vegetalitas mosquito repellent composition 60 ~ 85%, emulsifier 1~4%, moisturizer 1~8%, moisturizing agent 10~20%, counter-stimulus
0.1~1%, pH adjusting agent 0.1~0.5%, preservative 0.01~0.05%, odorant 0.1~0.5%.
Above-mentioned raw materials are mixed in proportion when preparation, 100% is added to distilled water, is emulsified, then homogeneous 5~8
Minute, that is, it is prepared into mosquito-dispelling cream.Then it samples and the stability of preparation is detected with appearance, packed to get finished product after qualified.
In a specific embodiment, Mosquito-repellant spray includes following components（Following mass percents are whole with spray
On the basis of body）：Vegetalitas mosquito repellent composition 45 ~ 65% of the present invention, emulsifier 1~5%, moisturizer 1~8%, moisturizing agent 1~4%,
Counter-stimulus 0.1~1%, PH conditioning agents 0.1~0.6%, preservative 0.01~0.1%, odorant 0.1~0.5%, remaining for distillation
Water.
Above-mentioned raw materials are mixed in proportion when manufacture, 100% is added to distilled water, is emulsified, then homogeneous 3~5
Min is prepared into Mosquito-repellant spray.Then sample and the stability of preparation be detected with appearance, it is qualified after packaging to get at
Product.
The present invention is described further for following embodiment, rather than limits the scope of the invention.
Embodiment 1, the preparation of mosquito-dispelling cream and indoor repellent Culex quinquefasciatus experiment
Mosquito-dispelling cream is made by following methods：It is vegetalitas mosquito repellent composition 70% of the present invention, emulsifier to take mass percent
2%, moisturizer 7%, moisturizing agent 15%, counter-stimulus 0.5%, pH adjusting agent 0.25%, preservative 0.05%, odorant 0.2%.It will be upper
It states raw material to mix in proportion, 100% is added to distilled water, be pumped into emulsification pot and emulsified, then homogeneous 5~8 minutes, i.e.,
It is prepared into mosquito-dispelling cream, then samples and the stability of preparation is detected with appearance, is packed to get finished product after qualified.
Vegetalitas mosquito repellent composition described above touches essential oil mass percent 55%, arborvitae essential oil 20%, the five colors by one
Plum essential oil 20%, be added 5% ethyl alcohol dissolving mix.Emulsifier described above is polyethenoxy ether class, and the moisturizing agent is
Synthetic Oil, the amino acid, the counter-stimulus are α-bisabolol, and the pH adjusting agent is citric acid.
In laboratory conditions（Laboratory room is the m of 4 m × 4m × 3, and temperature is 25 °C ± 2 °C, and humidity is
70% ± 5%）, with reference to National Standard of the People's Republic of China's " indoor harmacological effect evaluation/repellent of agriculture chemical registration hygienic insecticide
Agent GB/T 13917.9-2009 " test repellent effect.Test the mosquito cage of 3 days Culex quinquefasciatus after sprouting wings equipped with 100（30
cm × 30 cm × 25 cm）Middle progress, 4 volunteers will be put on after both hands wash clean the back of the hand position there are one 4cm ×
The medical latex glove of 4 cm notches puts his hand into the attack of mosquito cage 2 min test mosquitoes（Stop falling to count and be considered as 20 or more
Qualification, this person and this mosquito cage can be used for repellent experiment）.One hand is blank control when experiment, and another hand is directly naked in the back of the hand
Reveal position and smear mosquito-dispelling cream, dosage used is 3 mg/cm2.It puts his hand into 2 min of mosquito cage after natural air drying to be tested, every 0.5
H tests are primary, continue until observing that first mosquito stops falling or attempting to bite, are considered as complete shielding failure.Experiment repeats 3
Secondary, experimental result takes the average value tested every time.Measure mosquito-dispelling cream is to the 100% complete guard time average value of volunteer
6.56 ± 0.15 h。
Embodiment 2, the preparation of Mosquito-repellant spray and indoor repellent experiment
The Mosquito-repellant spray of experiment is made by following methods：Mass percent is taken to be combined for vegetalitas culicifuge of the present invention
Object 58%, moisturizer 5%, moisturizing agent 2%, counter-stimulus 0.25%, pH adjusting agent 0.6%, preservative 0.05%, assigns perfume at emulsifier 5%
Agent 0.1%, remaining be distilled water.Above-mentioned raw materials are mixed in proportion when manufacture, 100% is added to distilled water, is pumped into emulsification
Pot is emulsified, and then 3~5 min of homogeneous, that is, be prepared into Mosquito-repellant spray.Then sample to the stability of preparation and appearance into
Row detection is packed after qualified to get finished product.
Vegetalitas mosquito repellent composition described above touches essential oil mass percent 55%, arborvitae essential oil 20%, the five colors by one
Plum essential oil 20%, be added 5% ethyl alcohol dissolving mix.Emulsifier described above is polyethenoxy ether class, and the moisturizing agent is
Synthetic Oil, the amino acid, the counter-stimulus are α-bisabolol, and the pH adjusting agent is citric acid.
In laboratory conditions（Laboratory room is the m of m × 3 of 4 m × 4, and temperature is 25 °C ± 2 °C, and humidity is
70% ± 5%）, with reference to National Standard of the People's Republic of China's " indoor harmacological effect evaluation/repellent of agriculture chemical registration hygienic insecticide
Agent GB/T 13917.9-2009 " test repellent effect.Test the mosquito cage of 3 days Culex quinquefasciatus after sprouting wings equipped with 100（30
cm × 30 cm × 25 cm）Middle progress, 4 volunteers will be put on after both hands wash clean the back of the hand position there are one 4cm ×
The medical latex glove of 4cm notches puts his hand into the attack of mosquito cage 2 min test mosquitoes（Stop falling to count and be considered as 20 or more
Qualification, this person and this mosquito cage can be used for repellent experiment）.One hand is blank control when experiment, and another hand is directly naked in the back of the hand
Reveal position and smear mosquito-dispelling cream, dosage used is 3 mg/cm2.It puts his hand into 2 min of mosquito cage after natural air drying to be tested, every 0.5
H tests are primary, continue until observing that first mosquito stops falling or attempting to bite, are considered as complete shielding failure.Experiment repeats 3
Secondary, experimental result takes the average value tested every time.Measure 100% complete guard time average value of the Mosquito-repellant spray to volunteer
For 4.75 ± 0.70 h.
Embodiment 3
Mosquito-dispelling cream is prepared as described in Example 1, and tests repellent effect.Except that each component content：Vegetalitas
Mosquito repellent composition 60%, emulsifier 3%, moisturizer 3%, moisturizing agent 15%, counter-stimulus 0.3%, pH adjusting agent 0.3%, preservative
0.05%, odorant 0.3%.Vegetalitas mosquito repellent composition：One touches essential oil mass percent 40%, arborvitae essential oil 25%, the five colors
Plum essential oil 30%, ethyl alcohol 5%.Test result：The 100% complete guard time average value to volunteer is 4.93 ± 0.37 h.
By the method for the present embodiment, essential oil, arborvitae essential oil, Lantana camara essential oil are touched with one respectively and replace the present embodiment formula
In vegetalitas mosquito repellent composition, prepare three kinds of single dose essential oil mosquito-dispelling creams, and test repelling effect.Obtained test result
It is：One touches fragrant mosquito-dispelling cream, arborvitae mosquito-dispelling cream, Lantana camara mosquito-dispelling cream is respectively to the 100% complete guard time average value of volunteer
2.25 ± 0.19 h、 3.26 ± 0.19 h、3.03 ± 0.23 h。
Test result shows the mosquito-dispelling cream prepared by vegetalitas mosquito repellent composition of the present invention than using in composition respectively
Mosquito-dispelling cream prepared by any single essential oil, repellent effect are remarkably reinforced.
Embodiment 4
Mosquito-repellant spray is prepared as described in Example 2, and tests repellent effect.Except that each component content：It plants
Physical property mosquito repellent composition 45%, emulsifier 3%, moisturizer 2%, moisturizing agent 3%, counter-stimulus 0.5%, pH adjusting agent 0.3%, anti-corrosion
Agent 0.05%, odorant 0.2%.Vegetalitas mosquito repellent composition：One touches essential oil mass percent 70%, arborvitae essential oil 15%, five
Color plum essential oil 10%, ethyl alcohol 5%.
Test result：The 100% complete guard time average value to volunteer is 3.87 ± 0.18 h.
By the method for the present embodiment, essential oil, arborvitae essential oil, Lantana camara essential oil are touched with one respectively and replace the present embodiment formula
In vegetalitas mosquito repellent composition, prepare three kinds of single dose essential oil Mosquito-repellant sprays, and test repelling effect.Obtained test
The result is that：One touches fragrant Mosquito-repellant spray, arborvitae Mosquito-repellant spray, Lantana camara Mosquito-repellant spray protects completely to the 100% of volunteer
Time average is 2.19 ± 0.07 h, 3.06 ± 0.09 h, 2.33 ± 0.14 h.
Test result shows the Mosquito-repellant spray prepared by vegetalitas mosquito repellent composition of the present invention than respectively using combination
The Mosquito-repellant spray that in object prepared by any single essential oil, repellent effect are remarkably reinforced.
Summarize the present invention：
By the repetition test of inventor, find to touch essential oil, arborvitae essential oil and Lantana camara essential oil by certain ratio by one
The composition that example compounding obtains has significant repellent action to Culex quinquefasciatus.In three vegetable oils that the present invention selects respectively
There are a plurality of types of repellency compounds, such as one touches in essential oil containing limonene, laurene, carypohyllene；Arborvitae essential oil
In containing elemene, cedrol, thujene, chamigrene etc.；Lantana camara essential oil contains alpha-terpineol, linalool, Cineole, Isosorbide-5-Nitrae-
Lauro lene, laurene etc.；Plants essential oil complex chemical composition, these three plants essence very unique to the repellent action mechanism of housefly
After oily mixture, pharmacologically mutually promote between active constituent.Vegetalitas mosquito repellent composition of the present invention is nontoxic degradable, adopts simultaneously
Auxiliary agent is all environment-friend auxiliaries, safe to the human body nontoxic environmentally friendly, and preparation method is simple, is easy to implement.
Claims (9)
1. a kind of vegetalitas culicifuge of repellent Culex quinquefasciatus, it is characterised in that the chief active object of the culicifuge is：Contain one
Touch the composition of essential oil, arborvitae essential oil and Lantana camara essential oil, the mass parts content of each component is one to touch essence in the composition
10 ~ 30 parts of 40 ~ 70 parts of oil, 15 ~ 30 parts of arborvitae essential oil and Lantana camara essential oil；Described one touches essential oil, arborvitae essential oil and Lantana camara
Essential oil is to touch to extract in spiceleaf, arborvitae branches and leaves and the wounded in the battle branches and leaves of Lantana camara from one respectively by steam distillation to obtain.
2. the vegetalitas culicifuge of repellent Culex quinquefasciatus according to claim 1, it is characterised in that the composition also wraps
Solvent is included, solvent accounts for the 5 ~ 10% of the composition total weight.
3. the vegetalitas culicifuge of the repellent Culex quinquefasciatus according to one of claim 1 ~ 2, it is characterised in that the culicifuge
For creme, the mass percentage content that the composition accounts for the creme is 60 ~ 85%.
4. the vegetalitas culicifuge of repellent Culex quinquefasciatus according to claim 3, it is characterised in that mosquito-dispelling cream further includes breast
Agent 1~4%, moisturizer 1~8%, moisturizing agent 10~20%, counter-stimulus 0.1~1%, pH adjusting agent 0.1~0.5%, preservative
0.01~0.05% and odorant 0.1~0.5%, remaining is distilled water, adds up to 100%, in terms of the mass percent for accounting for the creme.
5. the vegetalitas culicifuge of the repellent Culex quinquefasciatus according to one of claim 1 ~ 2, it is characterised in that the culicifuge
For spray, the mass percentage content that the composition accounts for the spray is 45 ~ 65%.
6. the vegetalitas culicifuge of repellent Culex quinquefasciatus according to claim 5, it is characterised in that spray further includes breast
Agent 1~5%, moisturizer 1~8%, moisturizing agent 1~4%, counter-stimulus 0.1~1%, pH adjusting agent 0.1~0.6%, preservative
0.01~0.1% and odorant 0.1~0.5%, remaining is distilled water, adds up to 100%, in terms of the mass percent for accounting for the spray.
7. the vegetalitas culicifuge of the repellent Culex quinquefasciatus according to claim 4 or 6, it is characterised in that the emulsifier
One or more of in polyethenoxy ether class, carboxylate, sulfate, polyoxypropylene ethers and sulfonate, moisturizer is polynary
One or more of in alcohol, hyaluronic acid and amino acid, the moisturizing agent is isomery dodecane, higher alcohol, vegetable and animals oils and conjunction
One or more of in grease, the counter-stimulus is α-bisabolol, and the pH adjusting agent is triethanolamine or citric acid.
8. the preparation method of vegetalitas culicifuge described in a kind of claim 1, it is characterised in that be uniformly mixed each raw material, breast
Change, homogeneous is made.
9. a kind of application in repellent Culex quinquefasciatus indoors of vegetalitas culicifuge described in claim 1.
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| CN109090151A (en) * | 2018-08-17 | 2018-12-28 | 威宁县农景种植专业合作社 | A kind of Chinese prickly ash mosquito-repellent spray and preparation method thereof |
| CN111758723A (en) * | 2020-07-20 | 2020-10-13 | 成都彩虹电器（集团）股份有限公司 | Synergist for electric heating mosquito-repellent incense tablet and electric heating mosquito-repellent incense tablet |
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| CN101390525A (en) * | 2008-11-07 | 2009-03-25 | 中南林业科技大学 | Plant source mosquito driving and killing agent |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101390525A (en) * | 2008-11-07 | 2009-03-25 | 中南林业科技大学 | Plant source mosquito driving and killing agent |
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