CN105941334A - Method for incubation and rapid separation of Artemia nauplii - Google Patents
Method for incubation and rapid separation of Artemia nauplii Download PDFInfo
- Publication number
- CN105941334A CN105941334A CN201610276290.1A CN201610276290A CN105941334A CN 105941334 A CN105941334 A CN 105941334A CN 201610276290 A CN201610276290 A CN 201610276290A CN 105941334 A CN105941334 A CN 105941334A
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- hatching
- artemia
- artemia nauplii
- nauplii
- mesh bag
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- 241000238582 Artemia Species 0.000 title claims abstract description 53
- 238000000034 method Methods 0.000 title claims abstract description 34
- 238000000926 separation method Methods 0.000 title claims abstract description 17
- 238000011534 incubation Methods 0.000 title abstract description 14
- 230000012447 hatching Effects 0.000 claims description 55
- 102000002322 Egg Proteins Human genes 0.000 claims description 21
- 108010000912 Egg Proteins Proteins 0.000 claims description 21
- 210000004681 ovum Anatomy 0.000 claims description 21
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 19
- 239000011152 fibreglass Substances 0.000 claims description 18
- 241000133262 Nauplius Species 0.000 claims description 14
- 210000001136 chorion Anatomy 0.000 claims description 12
- 239000013535 sea water Substances 0.000 claims description 11
- 208000031513 cyst Diseases 0.000 claims description 9
- 239000003623 enhancer Substances 0.000 claims description 9
- 235000016709 nutrition Nutrition 0.000 claims description 9
- 230000035764 nutrition Effects 0.000 claims description 9
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 claims description 6
- DTOSIQBPPRVQHS-PDBXOOCHSA-N alpha-linolenic acid Chemical compound CC\C=C/C\C=C/C\C=C/CCCCCCCC(O)=O DTOSIQBPPRVQHS-PDBXOOCHSA-N 0.000 claims description 6
- 239000013505 freshwater Substances 0.000 claims description 6
- 239000000843 powder Substances 0.000 claims description 6
- 238000002791 soaking Methods 0.000 claims description 6
- 239000004744 fabric Substances 0.000 claims description 4
- 239000004575 stone Substances 0.000 claims description 4
- WRIDQFICGBMAFQ-UHFFFAOYSA-N (E)-8-Octadecenoic acid Natural products CCCCCCCCCC=CCCCCCCC(O)=O WRIDQFICGBMAFQ-UHFFFAOYSA-N 0.000 claims description 3
- LQJBNNIYVWPHFW-UHFFFAOYSA-N 20:1omega9c fatty acid Natural products CCCCCCCCCCC=CCCCCCCCC(O)=O LQJBNNIYVWPHFW-UHFFFAOYSA-N 0.000 claims description 3
- QSBYPNXLFMSGKH-UHFFFAOYSA-N 9-Heptadecensaeure Natural products CCCCCCCC=CCCCCCCCC(O)=O QSBYPNXLFMSGKH-UHFFFAOYSA-N 0.000 claims description 3
- 239000004470 DL Methionine Substances 0.000 claims description 3
- 229920002430 Fibre-reinforced plastic Polymers 0.000 claims description 3
- ZQPPMHVWECSIRJ-UHFFFAOYSA-N Oleic acid Natural products CCCCCCCCC=CCCCCCCCC(O)=O ZQPPMHVWECSIRJ-UHFFFAOYSA-N 0.000 claims description 3
- 239000005642 Oleic acid Substances 0.000 claims description 3
- 108010001441 Phosphopeptides Proteins 0.000 claims description 3
- 108010073771 Soybean Proteins Proteins 0.000 claims description 3
- 229920002494 Zein Polymers 0.000 claims description 3
- 235000020661 alpha-linolenic acid Nutrition 0.000 claims description 3
- 229940071162 caseinate Drugs 0.000 claims description 3
- 239000012141 concentrate Substances 0.000 claims description 3
- 239000003365 glass fiber Substances 0.000 claims description 3
- 238000005286 illumination Methods 0.000 claims description 3
- QXJSBBXBKPUZAA-UHFFFAOYSA-N isooleic acid Natural products CCCCCCCC=CCCCCCCCCC(O)=O QXJSBBXBKPUZAA-UHFFFAOYSA-N 0.000 claims description 3
- 229960004488 linolenic acid Drugs 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 3
- FFEARJCKVFRZRR-UHFFFAOYSA-N methionine Chemical compound CSCCC(N)C(O)=O FFEARJCKVFRZRR-UHFFFAOYSA-N 0.000 claims description 3
- 229930182817 methionine Natural products 0.000 claims description 3
- 235000006109 methionine Nutrition 0.000 claims description 3
- ZQPPMHVWECSIRJ-KTKRTIGZSA-N oleic acid Chemical compound CCCCCCCC\C=C/CCCCCCCC(O)=O ZQPPMHVWECSIRJ-KTKRTIGZSA-N 0.000 claims description 3
- 235000019710 soybean protein Nutrition 0.000 claims description 3
- 239000005019 zein Substances 0.000 claims description 3
- 229940093612 zein Drugs 0.000 claims description 3
- 241001247197 Cephalocarida Species 0.000 claims 13
- 230000015572 biosynthetic process Effects 0.000 claims 1
- 241000251468 Actinopterygii Species 0.000 abstract description 3
- 238000005516 engineering process Methods 0.000 abstract description 3
- 238000009395 breeding Methods 0.000 abstract 2
- 230000001488 breeding effect Effects 0.000 abstract 2
- 244000144972 livestock Species 0.000 abstract 1
- 241000238426 Anostraca Species 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 241000595940 Notostraca Species 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 238000005273 aeration Methods 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 235000015170 shellfish Nutrition 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 241000238421 Arthropoda Species 0.000 description 1
- 241000238423 Branchiopoda Species 0.000 description 1
- 230000004308 accommodation Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000002203 pretreatment Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000007634 remodeling Methods 0.000 description 1
- 230000027272 reproductive process Effects 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention provides a method for incubation and rapid separation of Artemia nauplii. The method can realize high-level incubation rate and rapid separation of Artemia nauplii. According to a plurality of years of experiences of incubation and separation of Artemia in the process of fish breeding, directed at biological characteristics of Artemia, a novel scientific and reasonable method and technology with good operability for incubation and separation of Artemia are provided, so incubation rate and separation speed of Artemia nauplii are improved; and a stable and high-quality biological feed is provided for fry breeding of aquatic livestock.
Description
Technical field
The present invention relates to artemia nauplii hatching and isolation technics during aquatic product animal raise seedling, particularly to the hatching of a kind of artemia nauplii and the method for sharp separation.
Background technology
Artemia (Brine Shrimp) is also referred to as saline fairy shrimp, belongs to Arthropoda, Branchiata, Crustachia, Branchiopoda, Anostraca, saline fairy shrimp section, is seawater fish and the preferable biological feed of shell-fish, is constantly subjected to the extensive attention of people.The hatching water temperature of artemia is 25~30 DEG C, and salinity is 20~30, and the generally employing that separates of artemia nauplii stands the method combined with photo-induction.After the hatching of worm's ovum completes, artemia nauplii be collected in incubation vessel.First gas tube, gas stone being taken out from couveuse, cover one block of black cloth, make in cylinder in dark state on couveuse top, from container bottom siphon nauplius with do not hatch the mixture of ovum in bolting silk net after 10~15min, this process should be avoided being mixed into ghost as far as possible.After nauplius collects, in addition it is also necessary to being separated by the worm's ovum of the ghost being mixed into and end hatching, otherwise ghost is eaten by fry and can be caused large quantities of death.At present, separation method mainly has light to separate to separate with proportion, though these two kinds of methods can complete the separation of artemia larvae, but existence duration, separate halfway drawback.Finding out a separating rate fast, the separation of artemia larvae is very important by the new method that point interest rate is high.
Summary of the invention
It is an object of the invention to provide the hatching of a kind of artemia nauplii and the method for sharp separation, the method can realize the incubation rate of the higher level of artemia nauplii and quickly separate.
The technical solution adopted for the present invention to solve the technical problems is:
The hatching of a kind of artemia nauplii and the method for sharp separation, the method comprises the steps:
1., before hatching, artemia cysts processes
The glass fibre reinforced plastic groove making apparatus conical bottom is hatched, and artemia cysts with freshwater soaking 1 to 3 hour, makes worm's ovum fully absorb water before hatching, before hatching sterilizes worm's ovum, with 2~the dipped into formalin 10~15min of 3%;
2., artemia ovum hatching
Hatching water temperature maintains 25~30 DEG C, best 28 DEG C;Salinity is the sea water of 20~30;PH value is 7.5~8.5;Gas stone is placed in the bottom of hatching cylinder, continuous charge in hatching process, water body is made to roll, avoid forming dead angle at cylinder bottom, the density of high-quality worm's ovum (incubation rate more than 85%) is 3-4g/L, and artemia cysts freshwater soaking carries out illumination (general use 2000lx artificial lighting to illuminate above hatching cylinder) in lh after fully absorbing water;
3., artemia nauplii fortification
Under these conditions, start broken shell through about 25h artemia nauplii, in joining hatching barrel after being now sufficiently stirred for agitated for artemia nutrition enhancer machine dissolving, carry out fortification;
4., artemia nauplii rapidly and efficiently separates
As preferably, step process 4. is:
First, preparing the fiberglass tank having conical bottom and long 80cm, 60 mesh sieve thin,tough silk bags of diameter 30cm, bottom of gullet has flowing water Valve, has inlet valve above, adds the clean sea water of 2/3 in tank;
Secondly, hatching cylinder, after adding nutrition enhancer 12 hours, stops inflation, and covers with black cloth at hatching cylinder top, stand 10 minutes;Slowly opening hatching cylinder bottom end, drained by the ovum of not hatching flowed out at first, on outlet set, the bolting silk mesh bag of 120 mesh, collects nauplius;When in container, liquid level drops to conical lower portion, take bolting silk mesh bag away, chorion is drained;Nauplius in bolting silk bag is transferred in the bucket equipped with clean sea water;
Again, in in appropriate bucket, the artemia nauplii of band shell pours bolting silk bag into, the right hand holds bolting silk bag mouth, firmly in ready fiberglass tank whipping for several times after, open inlet valve, the nauplius and chorion rinsed in mesh bag is concentrated with current, it is allowed to concentrate on the bottom of mesh bag, continue firmly that whipping is for several times in fiberglass tank, after 2-3 time is repeated, nauplius in mesh bag can be thrown in fiberglass tank, chorion is stayed in mesh bag, outwell the chorion in mesh bag, add artemia nauplii to be separated, continue to separate by this method, after whole artemia naupliis has been separated, open the flowing water Valve of fiberglass tank, the artemia nauplii of separator well in fiberglass tank is collected with the bolting silk mesh bag of 120 mesh, after fully rinsing, put into Miao Chizhong throw something and feed.
As preferably, the formula of nutrition enhancer is: DL-methionine 1.2g/L, oleic acid 0.5 g/L, Zein powder 3 g/L, soybean protein powder 3 g/L, phosphopeptide caseinate 0.3 g/L, alpha-linolenic acid 0.2 g/L.
As preferably, step 2. in, DO is maintained the level of 2mg/L, available optimal hatching effect.Aeration quantity should suitably be controlled, make worm's ovum can be uniformly distributed and be avoided that mechanical injuries are advisable.
Artemia is as indispensable biological feed in seawater fish and shell-fish seedling raising process, and it is hatched pre-treatment, hatches, separates and strengthen incubation rate and the survival rate all directly affecting artemia.The invention has the beneficial effects as follows: the present invention is according to the hatching of artemia in Fish reproductive process for many years and separating experience, for artemia biological characteristics, propose scientific and reasonable, workable artemia hatching and novel method for separating and technology, to improve incubation rate and the germling separating rate of artemia larvae, breed for aquatic animal fry and the biological feed stablizing high-quality is provided.
Detailed description of the invention
Below by specific embodiment, technical scheme is described in further detail.Should be appreciated that the enforcement of the present invention is not limited to the following examples, any pro forma accommodation and/or the change of being made the present invention fall within scope.
In the present invention, if not refering in particular to, all of part, percentage ratio are unit of weight, and the equipment used and raw material etc. are all commercially available or commonly used in the art.Method in following embodiment, if no special instructions, is the conventional method of this area.
Embodiment:
1, before hatching, artemia cysts processes
The glass fibre reinforced plastic groove making apparatus conical bottom is hatched, and hatching cylinder is with front carrying out disinfection.Artemia cysts with freshwater soaking 1 to 3 hour, makes worm's ovum fully absorb water before hatching, before hatching sterilizes worm's ovum, with 2~the dipped into formalin 10~15min of 3%.
2, artemia ovum hatching
Hatching water temperature maintains 25~30 DEG C;Salinity is the sea water of 20~30;PH value is preferred with 7.5~8.5.Place enough gas stone in the bottom of hatching cylinder, hatching process needs continuous charge, makes water body roll, it is to avoid form dead angle at cylinder bottom.The level that DO maintains 2mg/L can get optimal hatching effect.Aeration quantity should suitably be controlled, make worm's ovum can be uniformly distributed and be avoided that mechanical injuries are advisable.The worm's ovum density that the density of high-quality worm's ovum (incubation rate more than 85%) typically uses is 3g/L.Artemia cysts freshwater soaking fully absorb water after lh in illumination be important to improving incubation rate, general use 2000lx artificial lighting to illuminate above hatching cylinder.
3, artemia nauplii fortification
Under these conditions, start broken shell through about 25h artemia nauplii, in joining hatching barrel after being now sufficiently stirred for agitated for artemia nutrition enhancer machine dissolving, carry out fortification.
The formula of nutrition enhancer is: DL-methionine 1.2g/L, oleic acid 0.5 g/L, Zein powder 3 g/L, soybean protein powder 3 g/L, phosphopeptide caseinate 0.3 g/L, alpha-linolenic acid 0.2 g/L.
4, artemia nauplii rapidly and efficiently separates
First, preparing the fiberglass tank having conical bottom and long 80cm, 60 mesh sieve thin,tough silk bags of diameter 30cm, bottom of gullet has flowing water Valve, has inlet valve above, adds the clean sea water of 2/3 in tank.
Secondly, hatching cylinder, after adding nutrition enhancer 12 hours, stops inflation, and covers with black cloth at hatching cylinder top, stand 10 minutes.Slowly opening hatching cylinder bottom end, drained by the ovum of not hatching flowed out at first, on outlet set, the bolting silk mesh bag of 120 mesh, collects nauplius.When in container, liquid level drops to conical lower portion, take bolting silk mesh bag away, chorion is drained.Nauplius in bolting silk bag is transferred in the bucket equipped with clean sea water.
Again, in in appropriate bucket, the artemia nauplii of band shell pours bolting silk bag into, the right hand holds bolting silk bag mouth, firmly in ready fiberglass tank whipping for several times after, open inlet valve, the nauplius and chorion rinsed in mesh bag is concentrated with current, it is allowed to concentrate on the bottom of mesh bag, continue firmly that whipping is for several times in fiberglass tank, after 2-3 time is repeated, nauplius in mesh bag can be thrown in fiberglass tank, chorion is stayed in mesh bag, outwell the chorion in mesh bag, add artemia nauplii to be separated, continue to separate by this method, after whole artemia naupliis has been separated, open the flowing water Valve of fiberglass tank, the artemia nauplii of separator well in fiberglass tank is collected with the bolting silk mesh bag of 120 mesh, after fully rinsing, put into Miao Chizhong throw something and feed.
The incubation rate 96.8% of artemia.
Embodiment described above is the one preferably scheme of the present invention, and the present invention not makees any pro forma restriction, also has other variant and remodeling on the premise of without departing from the technical scheme described in claim.
Claims (4)
1. an artemia nauplii hatching and the method for sharp separation, it is characterised in that the method comprises the steps:
1., before hatching, artemia cysts processes
The glass fibre reinforced plastic groove making apparatus conical bottom is hatched, and artemia cysts with freshwater soaking 1 to 3 hour, makes worm's ovum fully absorb water before hatching, before hatching sterilizes worm's ovum, with 2~the dipped into formalin 10~15min of 3%;
2., artemia ovum hatching
Hatching water temperature maintains 25~30 DEG C;Salinity is the sea water of 20~30;PH value is 7.5~8.5;Place gas stone, continuous charge in hatching process in the bottom of hatching cylinder, make water body roll, it is to avoid at cylinder bottom formation dead angle, the density of high-quality worm's ovum is 3-4g/L, and artemia cysts freshwater soaking carries out illumination in lh after fully absorbing water;
3., artemia nauplii fortification
Under these conditions, start broken shell through about 25h artemia nauplii, in joining hatching barrel after being now sufficiently stirred for agitated for artemia nutrition enhancer machine dissolving, carry out fortification;
4., artemia nauplii rapidly and efficiently separates.
Artemia nauplii the most according to claim 1 hatching and the method for sharp separation, it is characterised in that step process 4. is:
First, preparing the fiberglass tank having conical bottom and long 80cm, 60 mesh sieve thin,tough silk bags of diameter 30cm, bottom of gullet has flowing water Valve, has inlet valve above, adds the clean sea water of 2/3 in tank;
Secondly, hatching cylinder, after adding nutrition enhancer 12 hours, stops inflation, and covers with black cloth at hatching cylinder top, stand 10 minutes;Slowly opening hatching cylinder bottom end, drained by the ovum of not hatching flowed out at first, on outlet set, the bolting silk mesh bag of 120 mesh, collects nauplius;When in container, liquid level drops to conical lower portion, take bolting silk mesh bag away, chorion is drained;Nauplius in bolting silk bag is transferred in the bucket equipped with clean sea water;
Again, in in appropriate bucket, the artemia nauplii of band shell pours bolting silk bag into, the right hand holds bolting silk bag mouth, firmly in ready fiberglass tank whipping for several times after, open inlet valve, the nauplius and chorion rinsed in mesh bag is concentrated with current, it is allowed to concentrate on the bottom of mesh bag, continue firmly that whipping is for several times in fiberglass tank, after 2-3 time is repeated, nauplius in mesh bag can be thrown in fiberglass tank, chorion is stayed in mesh bag, outwell the chorion in mesh bag, add artemia nauplii to be separated, continue to separate by this method, after whole artemia naupliis has been separated, open the flowing water Valve of fiberglass tank, the artemia nauplii of separator well in fiberglass tank is collected with the bolting silk mesh bag of 120 mesh, after fully rinsing, put into Miao Chizhong throw something and feed.
Artemia nauplii the most according to claim 1 hatching and the method for sharp separation, it is characterized in that: the formula of nutrition enhancer is: DL-methionine 1.2g/L, oleic acid 0.5 g/L, Zein powder 3 g/L, soybean protein powder 3 g/L, phosphopeptide caseinate 0.3 g/L, alpha-linolenic acid 0.2 g/L.
Artemia nauplii the most according to claim 1 hatching and the method for sharp separation, it is characterised in that: step 2. in, DO is maintained the level of 2mg/L.
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Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106879560A (en) * | 2017-04-18 | 2017-06-23 | 天津渤海水产研究所 | A kind of portable separator of indoor artemia hatching |
CN106912428A (en) * | 2016-10-13 | 2017-07-04 | 盘锦光合蟹业有限公司 | A kind of method of multi-biology cooperated preventing and treating river crab ecologic nursery pond harmful algae |
CN109618990A (en) * | 2018-12-04 | 2019-04-16 | 天津海友佳音生物科技股份有限公司 | A kind of incubation fluid and its hatching method of high density shelling slag oxygenation |
CN110301385A (en) * | 2019-08-16 | 2019-10-08 | 渤海水产股份有限公司 | A method of cultivation fairy shrimp |
CN114472168A (en) * | 2022-01-04 | 2022-05-13 | 山东省海洋科学研究院(青岛国家海洋科学研究中心) | Artemia grading device and grading screening method |
CN114698600A (en) * | 2022-03-28 | 2022-07-05 | 浙江省淡水水产研究所 | Method for efficiently purifying artemia used in shrimp seedling raising period |
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Cited By (8)
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CN106912428A (en) * | 2016-10-13 | 2017-07-04 | 盘锦光合蟹业有限公司 | A kind of method of multi-biology cooperated preventing and treating river crab ecologic nursery pond harmful algae |
CN106912428B (en) * | 2016-10-13 | 2020-06-09 | 盘锦光合蟹业有限公司 | Method for preventing and treating harmful algae in river crab ecological breeding pond through multi-organism synergy |
CN106879560A (en) * | 2017-04-18 | 2017-06-23 | 天津渤海水产研究所 | A kind of portable separator of indoor artemia hatching |
CN109618990A (en) * | 2018-12-04 | 2019-04-16 | 天津海友佳音生物科技股份有限公司 | A kind of incubation fluid and its hatching method of high density shelling slag oxygenation |
CN110301385A (en) * | 2019-08-16 | 2019-10-08 | 渤海水产股份有限公司 | A method of cultivation fairy shrimp |
CN114472168A (en) * | 2022-01-04 | 2022-05-13 | 山东省海洋科学研究院(青岛国家海洋科学研究中心) | Artemia grading device and grading screening method |
CN114472168B (en) * | 2022-01-04 | 2022-12-16 | 山东省海洋科学研究院(青岛国家海洋科学研究中心) | Artemia grading device and grading screening method |
CN114698600A (en) * | 2022-03-28 | 2022-07-05 | 浙江省淡水水产研究所 | Method for efficiently purifying artemia used in shrimp seedling raising period |
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