CN105934439B - 从生物流体中分离微小rna - Google Patents

从生物流体中分离微小rna Download PDF

Info

Publication number
CN105934439B
CN105934439B CN201480074125.7A CN201480074125A CN105934439B CN 105934439 B CN105934439 B CN 105934439B CN 201480074125 A CN201480074125 A CN 201480074125A CN 105934439 B CN105934439 B CN 105934439B
Authority
CN
China
Prior art keywords
mirna
sodium
binding protein
surfactant
mirnas
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201480074125.7A
Other languages
English (en)
Chinese (zh)
Other versions
CN105934439A (zh
Inventor
C.克里德
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sigma Aldrich Co LLC
Original Assignee
Sigma Aldrich Co LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sigma Aldrich Co LLC filed Critical Sigma Aldrich Co LLC
Priority to CN201910746637.8A priority Critical patent/CN110511924B/zh
Publication of CN105934439A publication Critical patent/CN105934439A/zh
Application granted granted Critical
Publication of CN105934439B publication Critical patent/CN105934439B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C07H1/06—Separation; Purification
    • C07H1/08—Separation; Purification from natural products
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21014—Microbial serine proteases (3.4.21.14)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/566—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/178—Oligonucleotides characterized by their use miRNA, siRNA or ncRNA

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
CN201480074125.7A 2013-11-27 2014-11-25 从生物流体中分离微小rna Active CN105934439B (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201910746637.8A CN110511924B (zh) 2013-11-27 2014-11-25 从生物流体中分离微小rna

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201361909834P 2013-11-27 2013-11-27
US61/909,834 2013-11-27
US201461985000P 2014-04-28 2014-04-28
US61/985,000 2014-04-28
PCT/US2014/067321 WO2015094609A1 (en) 2013-11-27 2014-11-25 Micro rna isolation from biological fluid

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CN201910746637.8A Division CN110511924B (zh) 2013-11-27 2014-11-25 从生物流体中分离微小rna

Publications (2)

Publication Number Publication Date
CN105934439A CN105934439A (zh) 2016-09-07
CN105934439B true CN105934439B (zh) 2019-09-10

Family

ID=53403495

Family Applications (2)

Application Number Title Priority Date Filing Date
CN201480074125.7A Active CN105934439B (zh) 2013-11-27 2014-11-25 从生物流体中分离微小rna
CN201910746637.8A Active CN110511924B (zh) 2013-11-27 2014-11-25 从生物流体中分离微小rna

Family Applications After (1)

Application Number Title Priority Date Filing Date
CN201910746637.8A Active CN110511924B (zh) 2013-11-27 2014-11-25 从生物流体中分离微小rna

Country Status (10)

Country Link
US (2) US10131902B2 (enExample)
EP (2) EP3702361B1 (enExample)
JP (1) JP6644686B2 (enExample)
KR (1) KR102181103B1 (enExample)
CN (2) CN105934439B (enExample)
BR (1) BR112016011770B1 (enExample)
CA (1) CA2930704C (enExample)
ES (2) ES2778223T3 (enExample)
IL (1) IL245646B (enExample)
WO (1) WO2015094609A1 (enExample)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105934439B (zh) 2013-11-27 2019-09-10 西格马-奥尔德里奇有限责任公司 从生物流体中分离微小rna
CN107385014B (zh) * 2017-06-13 2020-12-22 深圳大学 一种直接定量检测循环miRNA的RT-qPCR方法
KR102003594B1 (ko) 2017-08-08 2019-07-24 숙명여자대학교산학협력단 염증 반응 매개 신혈관생성 질환 진단용 조성물
CN111278844B (zh) * 2017-09-13 2023-12-19 贝克顿·迪金森公司 用于使用氧化铁颗粒提取核酸的方法和组合物
KR102811336B1 (ko) 2018-03-22 2025-05-21 아이덱스 래보러토리즈, 인코포레이티드 생물학적 샘플에서 박테리아를 측정하기 위한 방법
CN109490533B (zh) * 2018-12-29 2022-02-15 南京澳林生物科技有限公司 一种胃蛋白酶原ⅱ(pgⅱ)检测试剂盒及生产工艺
US20240312562A1 (en) * 2020-10-26 2024-09-19 Eclipse Bioinnovations, Inc. Methods and kits for enriching for polynucleotides
US11795500B2 (en) 2021-08-19 2023-10-24 Eclipse Bioinnovations, Inc. Methods for detecting RNA binding protein complexes
CN113980954A (zh) * 2021-11-11 2022-01-28 德州学院 一种病毒rna保护剂及其制备方法与应用

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2216406A1 (en) * 2007-11-30 2010-08-11 Wako Pure Chemical Industries, Ltd. Carrier, method and reagent for obtaining small rna
WO2012115885A1 (en) * 2011-02-22 2012-08-30 Caris Life Sciences Luxembourg Holdings, S.A.R.L. Circulating biomarkers
CN103119163A (zh) * 2010-09-24 2013-05-22 和光纯药工业株式会社 小rna的获得方法

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MXPA06003886A (es) * 2003-10-08 2006-08-11 Ebioscience Reactivos de enlace de inmunoglobulina naturales y metodos de fabricacion y uso de los mismos.
WO2010014636A1 (en) * 2008-07-28 2010-02-04 Rockefeller University Methods for identifying rna segments bound by rna-binding proteins or ribonucleoprotein complexes
US8846314B2 (en) * 2009-03-03 2014-09-30 The Board Of Trustees Of The Leland Stanford Junior University Isotachophoretic focusing of nucleic acids
EP3208338B1 (en) * 2011-08-04 2019-04-10 Yeda Research and Development Co., Ltd. Micro-rna mir-15 and compositions comprising same for the treatment of corticotropin-releasing hormone- associated medical conditions
CN102628038A (zh) * 2012-04-09 2012-08-08 武汉生命之美科技有限公司 一种改进的clip法鉴定rna结合蛋白的靶标rna的方法
WO2014082083A1 (en) * 2012-11-26 2014-05-30 Caris Science, Inc. Biomarker compositions and methods
US10435430B2 (en) * 2013-07-31 2019-10-08 Ionis Pharmaceuticals, Inc. Methods and compounds useful in conditions related to repeat expansion
CN105934439B (zh) 2013-11-27 2019-09-10 西格马-奥尔德里奇有限责任公司 从生物流体中分离微小rna
WO2016161375A2 (en) * 2015-04-03 2016-10-06 University Of Massachusetts Methods of using oligonucleotide-guided argonaute proteins

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2216406A1 (en) * 2007-11-30 2010-08-11 Wako Pure Chemical Industries, Ltd. Carrier, method and reagent for obtaining small rna
CN103119163A (zh) * 2010-09-24 2013-05-22 和光纯药工业株式会社 小rna的获得方法
WO2012115885A1 (en) * 2011-02-22 2012-08-30 Caris Life Sciences Luxembourg Holdings, S.A.R.L. Circulating biomarkers

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
CLIP-based prediction of mammalian microRNA binding sites;Chaochun Liu等;《Nucleic Acids Research》;20130522;第41卷(第14期);e138，第1-13页 *

Also Published As

Publication number Publication date
EP3702361B1 (en) 2022-08-31
CA2930704C (en) 2023-02-21
EP3702361A1 (en) 2020-09-02
EP3074413B1 (en) 2020-01-22
US10711266B2 (en) 2020-07-14
KR102181103B1 (ko) 2020-11-23
CA2930704A1 (en) 2015-06-25
BR112016011770B1 (pt) 2022-10-25
ES2778223T3 (es) 2020-08-10
EP3074413A1 (en) 2016-10-05
CN105934439A (zh) 2016-09-07
JP2017501689A (ja) 2017-01-19
US10131902B2 (en) 2018-11-20
US20160376582A1 (en) 2016-12-29
JP6644686B2 (ja) 2020-02-12
IL245646B (en) 2018-06-28
ES2930344T3 (es) 2022-12-12
WO2015094609A1 (en) 2015-06-25
CN110511924A (zh) 2019-11-29
IL245646A0 (en) 2016-06-30
US20190144850A1 (en) 2019-05-16
BR112016011770A2 (enExample) 2017-08-08
CN110511924B (zh) 2023-11-03
EP3074413A4 (en) 2017-05-31
KR20160089398A (ko) 2016-07-27

Similar Documents

Publication Publication Date Title
CN105934439B (zh) 从生物流体中分离微小rna
CA2705267C (en) Method for isolation of genomic dna, rna and proteins from a single sample
JP7573437B2 (ja) 膜タンパク質を使用する治療用エクソソームの調製
CN103764827B (zh) 可用于分离和／或纯化核酸的试剂
CN1685063B (zh) 与基因沉默相关的方法和组合物
CN113388606A (zh) 用于分离细胞外核酸的快速方法
JP6758315B2 (ja) 精子核酸の捕捉方法
US20130197206A1 (en) Method for acquisition of small rna
US20050214765A1 (en) Process for isolating nucleic acid with chaotrope agents and ammonium compounds
US20240375024A1 (en) Methods of isolating a composition comprising a target molecule
US20100311958A1 (en) Carrier, method and reagent for obtaining small rna
EP3296397A1 (en) Rev-clip, a new approach to identify proteins interacting with rna
WO2017026532A1 (ja) 抗原特異的モノクローナル抗体作製方法
WO2025197978A1 (ja) 核酸の回収方法

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant