CN105874069B - 改进的dna聚合酶寡核苷酸抑制剂 - Google Patents

改进的dna聚合酶寡核苷酸抑制剂 Download PDF

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CN105874069B
CN105874069B CN201480069584.6A CN201480069584A CN105874069B CN 105874069 B CN105874069 B CN 105874069B CN 201480069584 A CN201480069584 A CN 201480069584A CN 105874069 B CN105874069 B CN 105874069B
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double
chain
oligonucleotides
uracil
dna
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CN105874069A (zh
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E·H·菲斯
J·桑菲里普波
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F Hoffmann La Roche AG
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/99—Enzyme inactivation by chemical treatment
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
    • C12N2310/531—Stem-loop; Hairpin

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
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  • Wood Science & Technology (AREA)
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  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN201480069584.6A 2013-12-20 2014-12-18 改进的dna聚合酶寡核苷酸抑制剂 Active CN105874069B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361919520P 2013-12-20 2013-12-20
US61/919520 2013-12-20
PCT/EP2014/078350 WO2015091720A1 (en) 2013-12-20 2014-12-18 An improved oligonucleotide inhibitor of dna polymerases

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CN105874069A CN105874069A (zh) 2016-08-17
CN105874069B true CN105874069B (zh) 2019-01-18

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US (2) US10036061B2 (enExample)
EP (1) EP3083961B1 (enExample)
JP (1) JP6608826B2 (enExample)
CN (1) CN105874069B (enExample)
CA (1) CA2930926C (enExample)
WO (1) WO2015091720A1 (enExample)

Families Citing this family (11)

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Publication number Priority date Publication date Assignee Title
JP6490576B2 (ja) * 2012-04-12 2019-03-27 ニユー・イングランド・バイオレイブス・インコーポレイテツド 重合反応のための合成核酸
EP3464596B1 (en) * 2016-06-07 2025-07-30 Cepheid Thermostable polymerase inhibitor compositions and methods
US10669580B2 (en) * 2016-08-26 2020-06-02 Roche Sequencing Solutions, Inc. Tagged nucleotides useful for nanopore detection
US10724017B1 (en) * 2017-12-06 2020-07-28 Cepheid Inhibition of DNA polymerases by uracil-DNA glycosylase-cleavable oligonucleotide ligands
US10689629B1 (en) 2017-12-06 2020-06-23 Cepheid Inhibition of nucleic acid polymerases by endonuclease V-cleavable circular oligonucleotide ligands
US10724083B1 (en) 2017-12-06 2020-07-28 Cepheid Inhibition of nucleic acid polymerases by endonuclease V-cleavable oligonucleotide ligands
CN112251441B (zh) * 2020-10-26 2021-08-17 集美大学 一种具有靶向抑制鳗弧菌的核酸适配体h7及其应用
WO2023092128A2 (en) * 2021-11-22 2023-05-25 Integrated Dna Technologies, Inc. Aptamers for the reversible inhibition of dna polymerases
JP7514460B2 (ja) * 2022-03-31 2024-07-11 邦明 長峯 ポリアミンの検出方法、ポリアミンの検出試薬及びアプタマ
WO2026136820A1 (en) * 2024-12-20 2026-06-25 Cepheid Dna polymerase inhibitor
WO2026164135A1 (ja) * 2025-01-30 2026-08-06 国立研究開発法人産業技術総合研究所 耐熱性ｄｎａポリメラーゼの活性を阻害するオリゴヌクレオチドアプタマー

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1466018B2 (en) * 2002-01-08 2015-08-12 Roche Diagnostics GmbH Use of silica material in an amplification reaction
AU2006269282B2 (en) * 2005-07-07 2013-10-24 Quanta Biosciences, Inc. Compositions and methods for increasing amplification efficiency
US8669061B2 (en) * 2008-06-26 2014-03-11 Roche Molecular Systems, Inc. Method for the prevention of carryover contamination in nucleic acid amplification technologies
CA2688174C (en) 2008-12-19 2018-08-07 F. Hoffmann-La Roche Ag Dry composition of reaction compounds with stabilized polymerase

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CA2930926C (en) 2020-06-02
WO2015091720A1 (en) 2015-06-25
US10683540B2 (en) 2020-06-16
EP3083961A1 (en) 2016-10-26
US20180291429A1 (en) 2018-10-11
CA2930926A1 (en) 2015-06-25
JP6608826B2 (ja) 2019-11-20
US20150176059A1 (en) 2015-06-25
EP3083961B1 (en) 2018-07-18
CN105874069A (zh) 2016-08-17
JP2016540517A (ja) 2016-12-28
US10036061B2 (en) 2018-07-31

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