CN105866303A - 一种基于原位衍生测定多种神经递质的检测方法 - Google Patents
一种基于原位衍生测定多种神经递质的检测方法 Download PDFInfo
- Publication number
- CN105866303A CN105866303A CN201610466429.9A CN201610466429A CN105866303A CN 105866303 A CN105866303 A CN 105866303A CN 201610466429 A CN201610466429 A CN 201610466429A CN 105866303 A CN105866303 A CN 105866303A
- Authority
- CN
- China
- Prior art keywords
- acid
- liquid
- neurotransmitter
- solution
- rhodamine
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
- G01N2030/062—Preparation extracting sample from raw material
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
- G01N2030/067—Preparation by reaction, e.g. derivatising the sample
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
Abstract
Description
Claims (10)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201610466429.9A CN105866303B (zh) | 2016-06-24 | 2016-06-24 | 一种基于原位衍生测定多种神经递质的检测方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201610466429.9A CN105866303B (zh) | 2016-06-24 | 2016-06-24 | 一种基于原位衍生测定多种神经递质的检测方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN105866303A true CN105866303A (zh) | 2016-08-17 |
CN105866303B CN105866303B (zh) | 2018-05-25 |
Family
ID=56655502
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201610466429.9A Active CN105866303B (zh) | 2016-06-24 | 2016-06-24 | 一种基于原位衍生测定多种神经递质的检测方法 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN105866303B (zh) |
Cited By (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106248838A (zh) * | 2016-10-25 | 2016-12-21 | 杭州佰辰医学检验所有限公司 | 高通量液相色谱串联质谱的检测方法以及检测4种儿茶酚胺代谢物的方法 |
CN106645516A (zh) * | 2016-12-29 | 2017-05-10 | 香港科技大学 | 毛发中神经递质的检测方法 |
CN107449841A (zh) * | 2017-07-20 | 2017-12-08 | 上海药明康德新药开发有限公司 | 一种基于衍生化测定单胺类神经递质的检测方法 |
CN107966520A (zh) * | 2017-11-07 | 2018-04-27 | 广东中科康仪生物技术有限公司 | 用液相色谱串联质谱技术检测儿茶酚胺及代谢产物的方法 |
CN108802250A (zh) * | 2018-08-28 | 2018-11-13 | 上海中医药大学 | 超高效液质联用检测脑微透析液中11种神经递质的方法 |
CN109142594A (zh) * | 2018-08-14 | 2019-01-04 | 美康生物科技股份有限公司 | 用于准确测定体液样本中儿茶酚胺和变肾上腺素的质谱试剂盒 |
CN111044642A (zh) * | 2019-12-31 | 2020-04-21 | 贵州医科大学 | 一种同时测定五种神经递质含量的方法 |
CN112630311A (zh) * | 2020-07-09 | 2021-04-09 | 深圳云合医药科技合伙企业(有限合伙) | 用于检测情感障碍的代谢标记物和试剂盒及使用方法 |
US20210389336A1 (en) * | 2020-06-04 | 2021-12-16 | Korea Institute Of Science And Technology | Method for simultaneous analysis of neurotransmitters and their metabolites based on derivatization |
CN114621182A (zh) * | 2020-12-10 | 2022-06-14 | 中国科学院深圳先进技术研究院 | 一种衍生化试剂及其合成方法以及基于maldi-ms原位分析单胺类神经递质的方法 |
CN115184525A (zh) * | 2022-09-13 | 2022-10-14 | 裕菁科技(上海)有限公司 | 同一样品中性质相差很大的化合物的同时检测方法 |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH1151934A (ja) * | 1997-07-31 | 1999-02-26 | Kagakuhin Kensa Kyokai | 生体成分の分析方法、及び生体成分分析装置 |
CN1210262A (zh) * | 1998-09-18 | 1999-03-10 | 中国科学院生态环境研究中心 | 胺羟酰氯色谱衍生试剂及其制备工艺 |
WO2008071937A1 (en) * | 2006-12-11 | 2008-06-19 | Glaxo Group Ltd. | Novel fluorescent kinase ligands and assays employing the same |
CN103884676A (zh) * | 2014-02-27 | 2014-06-25 | 浙江大学 | 一种中药材多指标成分含量的快速测定方法 |
CN104678028A (zh) * | 2015-02-12 | 2015-06-03 | 中国人民解放军军事医学科学院毒物药物研究所 | 生物原胺类神经递质的前处理和检测方法、及检测试剂盒 |
-
2016
- 2016-06-24 CN CN201610466429.9A patent/CN105866303B/zh active Active
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH1151934A (ja) * | 1997-07-31 | 1999-02-26 | Kagakuhin Kensa Kyokai | 生体成分の分析方法、及び生体成分分析装置 |
CN1210262A (zh) * | 1998-09-18 | 1999-03-10 | 中国科学院生态环境研究中心 | 胺羟酰氯色谱衍生试剂及其制备工艺 |
WO2008071937A1 (en) * | 2006-12-11 | 2008-06-19 | Glaxo Group Ltd. | Novel fluorescent kinase ligands and assays employing the same |
CN103884676A (zh) * | 2014-02-27 | 2014-06-25 | 浙江大学 | 一种中药材多指标成分含量的快速测定方法 |
CN104678028A (zh) * | 2015-02-12 | 2015-06-03 | 中国人民解放军军事医学科学院毒物药物研究所 | 生物原胺类神经递质的前处理和检测方法、及检测试剂盒 |
Non-Patent Citations (2)
Title |
---|
XIAN-EN ZHAO等: "Simultaneous determination of amino acid and monoamine neurotransmitters in PC12 cells and rats models of Parkinson’s disease using a sensitizing derivatization reagent by UHPLC–MS/MS", 《JOURNAL OF CHROMATOGRAPHY B》 * |
YONGRUI HE等: "In situ derivatization-ultrasound-assisted dispersive liquid–liquid microextraction for the determination of neurotransmitters in Parkinson’s rat brain microdialysates by ultra high performance liquid chromatography-tandem mass spectrometry", 《JOURNAL OF CHROMATOGRAPHY A》 * |
Cited By (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106248838A (zh) * | 2016-10-25 | 2016-12-21 | 杭州佰辰医学检验所有限公司 | 高通量液相色谱串联质谱的检测方法以及检测4种儿茶酚胺代谢物的方法 |
CN106645516B (zh) * | 2016-12-29 | 2019-06-07 | 香港科技大学 | 毛发中神经递质的检测方法 |
CN106645516A (zh) * | 2016-12-29 | 2017-05-10 | 香港科技大学 | 毛发中神经递质的检测方法 |
CN107449841A (zh) * | 2017-07-20 | 2017-12-08 | 上海药明康德新药开发有限公司 | 一种基于衍生化测定单胺类神经递质的检测方法 |
CN107449841B (zh) * | 2017-07-20 | 2020-07-17 | 上海药明康德新药开发有限公司 | 一种基于衍生化测定单胺类神经递质的检测方法 |
CN107966520A (zh) * | 2017-11-07 | 2018-04-27 | 广东中科康仪生物技术有限公司 | 用液相色谱串联质谱技术检测儿茶酚胺及代谢产物的方法 |
CN109142594A (zh) * | 2018-08-14 | 2019-01-04 | 美康生物科技股份有限公司 | 用于准确测定体液样本中儿茶酚胺和变肾上腺素的质谱试剂盒 |
CN108802250A (zh) * | 2018-08-28 | 2018-11-13 | 上海中医药大学 | 超高效液质联用检测脑微透析液中11种神经递质的方法 |
CN111044642A (zh) * | 2019-12-31 | 2020-04-21 | 贵州医科大学 | 一种同时测定五种神经递质含量的方法 |
US20210389336A1 (en) * | 2020-06-04 | 2021-12-16 | Korea Institute Of Science And Technology | Method for simultaneous analysis of neurotransmitters and their metabolites based on derivatization |
CN112630311A (zh) * | 2020-07-09 | 2021-04-09 | 深圳云合医药科技合伙企业(有限合伙) | 用于检测情感障碍的代谢标记物和试剂盒及使用方法 |
CN112630311B (zh) * | 2020-07-09 | 2023-07-18 | 深圳云合医药科技合伙企业(有限合伙) | 用于检测情感障碍的代谢标记物和试剂盒及使用方法 |
CN114621182A (zh) * | 2020-12-10 | 2022-06-14 | 中国科学院深圳先进技术研究院 | 一种衍生化试剂及其合成方法以及基于maldi-ms原位分析单胺类神经递质的方法 |
CN114621182B (zh) * | 2020-12-10 | 2023-10-03 | 中国科学院深圳先进技术研究院 | 一种衍生化试剂及其合成方法以及基于maldi-ms原位分析单胺类神经递质的方法 |
CN115184525A (zh) * | 2022-09-13 | 2022-10-14 | 裕菁科技(上海)有限公司 | 同一样品中性质相差很大的化合物的同时检测方法 |
Also Published As
Publication number | Publication date |
---|---|
CN105866303B (zh) | 2018-05-25 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN105866303A (zh) | 一种基于原位衍生测定多种神经递质的检测方法 | |
CN105866316B (zh) | 一种同时检测食品中氨基酸和生物胺的分析方法 | |
Greco et al. | Microdialysate analysis of monoamine neurotransmitters—a versatile and sensitive LC–MS/MS method | |
Smyth et al. | A critical evaluation of high performance liquid chromatography‐electrospray ionisation‐mass spectrometry and capillary electrophoresis‐electrospray‐mass spectrometry for the detection and determination of small molecules of significance in clinical and forensic science | |
Meng et al. | Application of dispersive liquid–liquid microextraction and CE with UV detection for the chiral separation and determination of the multiple illicit drugs on forensic samples | |
CN106018612B (zh) | 一种环境与食品中多种内分泌干扰物的检测分析方法 | |
US8319194B2 (en) | Drug detection equipment | |
CN104991019B (zh) | 生物检材中钩吻素甲和钩吻素子的液相色谱-串联质谱检测方法 | |
CN106596796B (zh) | 基于液液微萃取的gc-ms测卷烟烟气中芳香胺的方法 | |
Norouzi et al. | Determination of morphine and oxymorphone in exhaled breath condensate samples: application of microwave enhanced three–component deep eutectic solvent-based air–assisted liquid–liquid microextraction and derivatization prior to gas chromatography–mass spectrometry | |
Ji et al. | Development of a modified quick, easy, cheap, effective, rugged, and safe method based on melamine sponge for multi-residue analysis of veterinary drugs in milks by ultra-performance liquid chromatography tandem mass spectrometry | |
CN107505411A (zh) | 一种合相色谱‑串联质谱法测定电子烟烟液中降烟碱对映异构体含量的方法 | |
Jin et al. | Determination of bromadiolone in whole blood by high-performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry | |
CN109738565A (zh) | 一种测定保健食品中非法添加化合物的方法 | |
CN105092753A (zh) | 组合型胺类代谢标志物的应用及试剂盒与检测方法 | |
CN107228914A (zh) | 一种合相色谱‑串联质谱法测定无烟气烟草制品中降烟碱对映异构体含量的方法 | |
Świądro et al. | Development of a new method for drug detection based on a combination of the dried blood spot method and capillary electrophoresis | |
Jain et al. | Simple determination of dichlorvos in cases of fatal intoxication by gas Chromatography-Mass spectrometry | |
Zhang et al. | Effects of the ambient fine particulate matter (PM2. 5) exposure on urinary metabolic profiles in rats using UPLC-Q-TOF-MS | |
Duverneuil et al. | Liquid chromatography/photodiode array detection for determination of strychnine in blood: a fatal case report | |
CN106226423A (zh) | 一种分离测定唾液中吡嗪类和吡啶类物质的方法 | |
Wang et al. | Development of a rapid detection method for seven pesticides in cucumber using hollow fibre liquid phase microextraction and ion mobility spectrometry | |
CN102507794A (zh) | 一种采用尿液快速检测所中毒物的方法 | |
CN105158372B (zh) | 一种化妆品中尿刊酸及其乙酯的测定方法 | |
Bodur et al. | One step derivatization and dispersive liquid-liquid microextraction of hydroxychloroquine sulfate for its sensitive and accurate determination using GC–MS |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: A detection method based on in situ derivatization for the determination of multiple neurotransmitters Effective date of registration: 20211209 Granted publication date: 20180525 Pledgee: Zou Cheng Branch of industrial and Commercial Bank of China Ltd. Pledgor: Zoucheng Xinqi Property Management Co.,Ltd. Registration number: Y2021980014651 |
|
PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
TR01 | Transfer of patent right |
Effective date of registration: 20211213 Address after: 273500 No. 517, Chenggong Road, Zoucheng City, Jining City, Shandong Province Patentee after: Zoucheng Xinqi Property Management Co.,Ltd. Address before: 273165 Jingxuan West Road, Qufu City, Jining, Shandong Province, No. 57 Patentee before: QUFU NORMAL University |
|
TR01 | Transfer of patent right | ||
CP01 | Change in the name or title of a patent holder |
Address after: 273500 No. 517, Chenggong Road, Zoucheng City, Jining City, Shandong Province Patentee after: Shandong Zhurong Property Co., Ltd. Address before: 273500 No. 517, Chenggong Road, Zoucheng City, Jining City, Shandong Province Patentee before: Zoucheng Xinqi Property Management Co.,Ltd. |
|
CP01 | Change in the name or title of a patent holder |