CN105851062A - Microorganism growth-promoting disease-preventing conditioning agent as well as use and application method - Google Patents
Microorganism growth-promoting disease-preventing conditioning agent as well as use and application method Download PDFInfo
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- CN105851062A CN105851062A CN201610266112.0A CN201610266112A CN105851062A CN 105851062 A CN105851062 A CN 105851062A CN 201610266112 A CN201610266112 A CN 201610266112A CN 105851062 A CN105851062 A CN 105851062A
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- microorganism growth
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- Agronomy & Crop Science (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Virology (AREA)
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- Agricultural Chemicals And Associated Chemicals (AREA)
- Cultivation Of Plants (AREA)
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Abstract
The invention relates to a microorganism growth-promoting disease-preventing conditioning agent as well as use and an application method. The microorganism growth-promoting disease-preventing conditioning agent is Pb-4 fermentation liquid of bacillus amyloliquefaciens sp.; the microorganism growth-promoting disease-preventing conditioning agent can be added into peat and then the mixture is filled into a seedling culture disc; seeds are sowed and the emergence rate is improved; at the middle period of seedling culture, the microorganism growth-promoting disease-preventing conditioning agent can be diluted and then is sprayed for one time by utilizing a sprayer or a fertilization device, so as to promote the growth of germchits and prevent and control the diseases; and the microorganism growth-promoting disease-preventing conditioning agent can be used for shortening the seeding age and improving the quality of the germchits, is simple and feasible and is low in cost; and the labor force is saved so that the product is used for industrial seedling culture of vegetables.
Description
Technical field
The invention belongs to microorganism application technology agriculturally, relate generally to a kind of microorganism growth-promoting diseases prevention conditioner, purposes and application process thereof containing bacillus amyloliquefaciens fermentation liquid.
Background technology
Growing vegetables industry is agricultural the second pillar industry being only second to Farming industry, and the vegetable seedling of health plays conclusive effect in growing vegetables industry, cultivating healthy and strong seedling is the important step that vegetable produces, and the quality of seedling directly influences the quality and yield of vegetable.Therefore, the quality improving vegetable seedling has become promotion Development of Vegetable Industry, meets people's vital task to vegetable demand.Traditional seedling raising manners does not only take up a large amount of soil, and it is many to waste seed, recruitment, does not the most adapt to the needs of modern agriculture, will progressively be substituted by industrial seedling rearing.Industrial seedling rearing by its with kind less, the advantage such as recruitment province receives in nursery field and extensively to favor.From the point of view of present case, China's vegetables industry has begun to the production using industrial breeding technique to carry out seedling in a large number.But, the expansion produced along with Vegetables Factory Plantation Growing Seedlings, the problems such as emergence rate is low, seedling raise period length, disease are serious occur the most therewith, and therefore, the seedling quality improving Vegetables Factory Plantation Growing Seedlings is the key link of development growing vegetables industry.
The most domestic Vegetables Factory Plantation Growing Seedlings that microorganism conditioner is used for, thus improve emergence rate, promote that the generation of seedling early growth, prevention and control disease, the invention of raising seedling quality also do not have been reported that.
Summary of the invention
It is an object of the invention to the expansion overcoming existing Vegetables Factory Plantation Growing Seedlings to produce, the problems such as emergence rate is low, seedling raise period length, disease are serious, it is provided that a kind of microorganism growth-promoting diseases prevention conditioner, purposes and application process thereof containing bacillus amyloliquefaciens fermentation liquid.
The present inventor is by China's Patent No.: new application that the bacillus amyloliquefaciens strain of 201310032838.4 " a kind of the antibacterial bacillus amyloliquefaciens of growth-promoting, conditioner preparation method and applications " is carried out and purposes.
For realizing the purpose of the invention described above, the technical scheme is that
A kind of microorganism growth-promoting diseases prevention conditioner, is characterized in that: for the fermentation liquid of bacillus amyloliquefaciens Bacillus amyloliquefaciens sp.Pb-4.
Bacillus amyloliquefaciens viable count about 2～5 × 10 in described fermentation liquid9cfu/ml。
Described a kind of microorganism growth-promoting diseases prevention conditioner, is characterized in that: for Vegetables Factory Plantation Growing Seedlings.
The application process of described a kind of microorganism growth-promoting diseases prevention conditioner, is characterized in that: as adding the microorganism growth-promoting diseases prevention conditioner described in 5～50L in the peat of volume every cubic metre, fills seedlings nursing plate, sows.
The application process of described a kind of microorganism growth-promoting diseases prevention conditioner, it is characterized in that: when nursery mid-term, described microorganism growth-promoting diseases prevention conditioner is diluted 500～1000 times, and utilizes aerosol apparatus or fertilizer applicator to spray once, to promote that seedling early growth, prevention and control disease occur.
Substantive distinguishing features and the remarkable result of the present invention be:
Bacillus amyloliquefaciens in a kind of microorganism growth-promoting conditioner that the present invention provides can be grown in seedling rhizome portion surely, secrete multiple growth promotion and disease prevention material, promote seedling root system and the growth of plant, prevention and control disease occurs, the comparison that after using microorganism growth-promoting diseases prevention conditioner seedling stem is thick, plant height, Biomass etc. are less used all increases, use microorganism growth-promoting diseases prevention conditioner can improve emergence rate, promote seedling early growth, shorten seedling age, prevention and control disease occurs, improve seedling quality.
Present invention facilitates the seedling early growth of Vegetables Factory Plantation Growing Seedlings, improve emergence rate, shortening seedling age 3～5 days, prevention and control disease occurs, and improves seedling quality, and strong sprout, growth-promoting effect was obvious.For the microorganism growth-promoting diseases prevention conditioner application process of Vegetables Factory Plantation Growing Seedlings, simple, with low cost, recruitment is saved.
Detailed description of the invention
One microorganism growth-promoting diseases prevention conditioner of the present invention, for the fermentation liquid of bacillus amyloliquefaciens Bacillus amyloliquefaciens sp.Pb-4.Bacillus amyloliquefaciens viable count about 2～5 × 10 in described fermentation liquid9cfu/ml.It is mainly used in the growth-promoting diseases prevention strong sprout of Vegetables Factory Plantation Growing Seedlings.
Bacillus amyloliquefaciens used in the present invention (Bacillus amyloliquefaciens) Pb-4 bacterial strain is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center；Address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, deposit number is CGMCC N0.7155, and preservation date is on January 18th, 2013.
One, the application process of a kind of microorganism of present invention growth-promoting diseases prevention conditioner
(1), microorganism growth-promoting diseases prevention conditioner is joined (addition 5～50L conditioners in the peat of i.e. 1 cubic metre) in peat in the ratio 5～50 ‰ of volume, stir, add water in right amount, it is added to substrate grab agglomerating squeezing the water out and be advisable, fully after mixing, lid plastic foil stand 3-4 hour or overnight after, seedlings nursing plate can be filled, sow.
(2), when nursery mid-term, utilize aerosol apparatus or fertilizer applicator to spray the microorganism growth-promoting diseases prevention conditioner of once dilution 500～1000 times, promote seedling early growth.
Two, for the application effect of microorganism growth-promoting diseases prevention conditioner of Vegetables Factory Plantation Growing Seedlings
1, tomato seedling effect test
Test site: Shanxi medium green brightness new crop varieties promotes Specialty Co-operative Organization's industrial seedling rearing base
Conditioner 10ml/L is added in peat, carry out tomato seedling, 45 days investigation tomato seedling growing states of nursery are: the Seedling adding the process of microorganism growth-promoting diseases prevention conditioner is sturdy, high, blade is big, color is deep, well developed root system, plant height, stem be thick and plant fresh weight and dry weight all increased than the comparison without conditioner, is shown in Table 1.
Table 1 peat adds microorganism growth-promoting diseases prevention conditioner tomato seedling growing state
2, pepper seedling raising effect test
Test site: Shanxi medium green brightness new crop varieties promotes Specialty Co-operative Organization's industrial seedling rearing base
Result of the test: add conditioner 10ml/L and 40ml/L in peat, carry out pepper seedling raising, 50 days investigation Hot Pepper Seedling growing states of nursery are: the Seedling adding the process of microorganism growth-promoting diseases prevention conditioner is sturdy, high, blade is big, color is deep, well developed root system, plant height, stem are thick, leaf area and plant fresh weight and dry weight all increased than the comparison without conditioner, are shown in Table 2.
Table 2 peat adds microorganism growth-promoting diseases prevention conditioner Hot Pepper Seedling growing state
3, Caulis et Folium Brassicae capitatae nursery effect test
Test site: the rich vegetable variety Demonstration Garden industrial seedling rearing base of benefit, Sun Jia stockaded village, Xiaodian District, Shanxi province Taiyuan city
Result of the test: carry out Caulis et Folium Brassicae capitatae Mechanization sowing after adding microorganism growth-promoting diseases prevention conditioner by 10ml/L, 20ml/L variable concentrations in peat.Sow 7 days " Invest, Then Investigate " seed sprouting situations, from seeding ratio, add microorganism growth-promoting diseases prevention conditioner and can improve emergence rate；Caulis et Folium Brassicae capitatae Seedling growing state is observed in nursery 32 days: add the Seedling growth that microorganism growth-promoting diseases prevention conditioner processes fast, than without comparison seedling age shift to an earlier date 3-5 days；In terms of plant weights, during in peat, interpolation microorganism growth-promoting diseases prevention conditioner processes, the amount of plant above ground portion and root is all high than comparison, is shown in Table 3.
Table 3 peat adds variable concentrations microorganism growth-promoting diseases prevention conditioner Caulis et Folium Brassicae capitatae emerge and growing state
4, for the application effect of microorganism growth-promoting diseases prevention conditioner of Vegetables Factory Plantation Growing Seedlings
Conditioner is added in peat and sprays, all can promote plant strain growth in seedling stage, under reaching standard premise in strong sprout, peat add conditioner and can shift to an earlier date seedling age 3-5 days (table 4), reduce liquid manure consumption more than 10%, also reduce seedling-raising technique labor management expense more than 10%.
Table 4 adds microorganism growth-promoting diseases prevention conditioner and shortens seedling age situation
Claims (5)
1. a microorganism growth-promoting diseases prevention conditioner, is characterized in that: this microorganism growth-promoting diseases prevention conditioner is for solving
The fermentation liquid of bacillus amyloliquefaciens Bacillus amyloliquefaciens sp.Pb-4.
2. a kind of microorganism growth-promoting diseases prevention conditioner as claimed in claim 1, is characterized in that: described sends out
Bacillus amyloliquefaciens viable count about 2～5 × 10 in ferment liquid9cfu/ml。
The purposes of a kind of microorganism growth-promoting diseases prevention conditioner the most as claimed in claim 1, is characterized in that: use
In Vegetables Factory Plantation Growing Seedlings.
The application process of a kind of microorganism growth-promoting diseases prevention conditioner the most as claimed in claim 2, is characterized in that:
As the peat of volume every cubic metre adds the microorganism growth-promoting diseases prevention conditioner described in 5～50L, fill nursery
Dish, sows.
The application process of a kind of microorganism growth-promoting diseases prevention conditioner the most as claimed in claim 2, its feature
It is: when nursery mid-term that described microorganism growth-promoting diseases prevention conditioner is diluted 500～1000 times, and profit
Spray once with aerosol apparatus or fertilizer applicator, to promote that seedling early growth, prevention and control disease occur.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610266112.0A CN105851062A (en) | 2016-04-26 | 2016-04-26 | Microorganism growth-promoting disease-preventing conditioning agent as well as use and application method |
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| CN201610266112.0A CN105851062A (en) | 2016-04-26 | 2016-04-26 | Microorganism growth-promoting disease-preventing conditioning agent as well as use and application method |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1952116A (en) * | 2006-04-18 | 2007-04-25 | 兰州大学 | Bacillusamyloliquefaciens strain and application thereof |
| CN103074287A (en) * | 2013-01-28 | 2013-05-01 | 山西省农业科学院农业环境与资源研究所 | Growth-promoting antibacterial bacillus amyloliquefaciens sp., preparation method of conditioner and application |
| EP2820953A1 (en) * | 2013-07-02 | 2015-01-07 | Symborg, S.L. | Glomus iranicum var. tenuihypharum var. nov. strain and its use as biostimulant |
| CN105820820A (en) * | 2015-12-02 | 2016-08-03 | 山西省农业科学院农业环境与资源研究所 | Microbial soil conditioner capable of controlling successive cropping obstacles of sorghum, and purpose and application method thereof |
-
2016
- 2016-04-26 CN CN201610266112.0A patent/CN105851062A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1952116A (en) * | 2006-04-18 | 2007-04-25 | 兰州大学 | Bacillusamyloliquefaciens strain and application thereof |
| CN103074287A (en) * | 2013-01-28 | 2013-05-01 | 山西省农业科学院农业环境与资源研究所 | Growth-promoting antibacterial bacillus amyloliquefaciens sp., preparation method of conditioner and application |
| EP2820953A1 (en) * | 2013-07-02 | 2015-01-07 | Symborg, S.L. | Glomus iranicum var. tenuihypharum var. nov. strain and its use as biostimulant |
| CN105820820A (en) * | 2015-12-02 | 2016-08-03 | 山西省农业科学院农业环境与资源研究所 | Microbial soil conditioner capable of controlling successive cropping obstacles of sorghum, and purpose and application method thereof |
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Application publication date: 20160817 |