CN105820820A - Microbial soil conditioner capable of controlling successive cropping obstacles of sorghum, and purpose and application method thereof - Google Patents
Microbial soil conditioner capable of controlling successive cropping obstacles of sorghum, and purpose and application method thereof Download PDFInfo
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- CN105820820A CN105820820A CN201510875664.7A CN201510875664A CN105820820A CN 105820820 A CN105820820 A CN 105820820A CN 201510875664 A CN201510875664 A CN 201510875664A CN 105820820 A CN105820820 A CN 105820820A
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- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K17/00—Soil-conditioning materials or soil-stabilising materials
- C09K17/14—Soil-conditioning materials or soil-stabilising materials containing organic compounds only
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
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Abstract
The invention relates to a microbial soil conditioner capable of controlling successive cropping obstacles of sorghum, and a purpose and an application method thereof. The microbial soil conditioner can effectively overcome the problem of successive cropping obstacles of sorghum, is Bacillus amyloliquefaciens sp. Pb-4 fermentation broth and can be used for controlling and regulation of soil with successive cropping obstacles of sorghum. The application method is to apply a weak solution of the microbial soil conditioner to soil of sowing lines of sorghum or soil in root zone of sorghum. The method is simple, easily practicable, low in cost and saving in labor, can effectively overcome successive cropping obstacles of sorghum, exerts yield-increasing effect on sorghum, is of great significance to sorghum producing districts where crop rotation of sorghum is hard to carry out, and accords with demands of development of ecological agriculture and sustainable agriculture.
Description
Technical field
The invention belongs to microorganism application technology agriculturally, relate generally to the soil conditioner containing bacillus amyloliquefaciens fermentation liquid, purposes and application process thereof.
Background technology
Sorghum vulgare Pers. is one of important five big cereal crops in the whole world, and it is possible not only to eat, and can act also as the raw material of industry, brewing industry, feed industry；Utilizing sweet sorghum production biomass energy is also one of important measures tackling Global climate change.China's Sorghum vulgare Pers. sown area 670,000 hectares at present, yield is 2,700,000 tons, and essentialspecies is implanted in the ground such as the Inner Mongol, Jilin, Liaoning, Sichuan, Guizhou and Shanxi, mostly is brewing industry prosperity or the most barren region of soil；Sorghum vulgare Pers. in Moutai (Spirit) production base uses organic plantation, and Sorghum vulgare Pers. continuous cropping obstacle leverages the output of Maotai；In Shanxi, Guizhou, Hebei, the Sorghum vulgare Pers. plantation of the ground such as Sichuan mostly be made-to-order farming, plant Sorghum vulgare Pers. to complete made-to-order farming behind Sorghum vulgare Pers. plantation cooperative society's circulation soil year after year, cause Sorghum vulgare Pers. continuous cropping obstacle serious；Planting sweet sorghum also have preferable economic benefit at beach, sugar grass continuous cropping obstacle is the most serious.Therefore, Sorghum vulgare Pers. continuous cropping obstacle causes the obstacle that famous brand of wine produces, and affects the agricultural sustainable development in Sorghum vulgare Pers. plantation area, and solving Sorghum vulgare Pers. continuous cropping obstacle for this is to have vital meaning.
Though some successive planting resistant agents controlling continuous cropping obstacles are had the most domestic, as Chinese patent Authorization Notice No. is: CN101735818.B " microbial soil conditioner prepared by edible fungus bran and preparation method thereof ", it it is the conditioner utilizing bacillus megaterium, colloid bacillus cereus and Streptomyces hygrospinosus bacterium solution to prepare with the conditioner carrier mixing prepared by mushroom bran respectively；And for example Chinese Patent Application No. is: a kind of microorganism mix bacterium agent that 201210192739.8 " a kind of microbial soil conditioners and preparation method thereof " are made up of bacillus polymyxa, Bacillus licheniformis and bacillus subtilis；The patent that above is referred to microbial soil conditioner is mainly preparation method, and is the solid dosage forms of mixing after multiple bacterial strain ferments respectively, operates cumbersome, and for the problem of soil continuous cropping Sorghum vulgare Pers. obstacle, does not has solution the most always.
Summary of the invention
It is an object of the invention to provide the application process of a kind of microbial soil conditioner containing bacillus amyloliquefaciens fermentation liquid, after using microbial soil conditioner by this method, it is possible to effectively control Sorghum vulgare Pers. continuous cropping obstacle, effect is notable, and crop is had effect of increasing production；Relatively big to the Sorghum vulgare Pers. producing region meaning being difficult to crop rotation, meet the demand of the ecological agriculture and sustainable agriculture development.
The present invention is by China's Patent No.: new application that the bacillus amyloliquefaciens kind of 201310032838.4 " a kind of the antibacterial bacillus amyloliquefaciens of growth-promoting, conditioner preparation method and applications " is carried out and purposes.
For realizing the purpose of the invention described above, the technical scheme is that
A kind of microbial soil conditioner controlling Sorghum vulgare Pers. continuous cropping obstacle, is characterized in that: for the fermentation liquid of bacillus amyloliquefaciens Bacillus amyloliquefaciens sp.Pb-4.
Bacillus amyloliquefaciens viable count about 2～5 × 10 in described fermentation liquid9cfu/ml。
The microbial soil conditioner of described controlled Sorghum vulgare Pers. continuous cropping obstacle, is characterized in that: the soil for Sorghum vulgare Pers. continuous cropping obstacle regulates and controls.
The microbial soil conditioner application process of described controlled Sorghum vulgare Pers. continuous cropping obstacle, it is characterized in that: the described control for Sorghum vulgare Pers. continuous cropping obstacle, it is that the fermentation liquid of bacillus amyloliquefaciens Pb-4 is diluted 50～150 times, make microbial soil conditioner diluent, and be applied in the soil in Sorghum vulgare Pers. sowing Hang Huoqigen district.
The microbial soil conditioner application process of described controlled Sorghum vulgare Pers. continuous cropping obstacle, it is characterized in that: described is applied to Sorghum vulgare Pers. sowing row or its root region soil, control for Sorghum vulgare Pers. continuous cropping obstacle, it is before sowing, microbial soil conditioner diluent to be loaded in aerosol apparatus or fertilizer applicator behind whole ground, is applied to Sorghum vulgare Pers. sowing row.
The microbial soil conditioner application process of described controlled Sorghum vulgare Pers. continuous cropping obstacle, it is characterized in that: described is applied to Sorghum vulgare Pers. sowing row or its root region soil, control for Sorghum vulgare Pers. continuous cropping obstacle, it it is the different growing Sorghum vulgare Pers., microbial soil conditioner diluent is carried out pouring root use or with irrigation water, microbial soil conditioner is applied field, act on Sorghum vulgare Pers. root region soil.
The above-described microbial soil conditioner application process controlling Sorghum vulgare Pers. continuous cropping obstacle, is characterized in that: use every time, and every mu of Sorghum vulgare Pers. cultivated area uses microbial soil conditioner diluent 100～300L.
Substantive distinguishing features and the remarkable result of the present invention be:
The problem that the present invention solves the most unsolved Sorghum vulgare Pers. soil continuous cropping obstacle.
Can control the microbial soil conditioner application process of Sorghum vulgare Pers. continuous cropping obstacle, simple, with low cost, recruitment is saved, and can effectively control Sorghum vulgare Pers. continuous cropping obstacle, and effect is notable, and crop is had effect of increasing production, bigger to the Sorghum vulgare Pers. producing region meaning being difficult to crop rotation.
Bacillus amyloliquefaciens in the microbial soil conditioner that the present invention provides can be grown surely in root of the crop stem, secrete multiple growth promoting substance, promote root system of plant and the growth of plant, the comparison that after using microbial soil conditioner, Sorghum Stalk Diameter, plant height, leaf area, Biomass and yield are less used all increases, use microbial soil conditioner scalable Soil structure and soil microenvironment, control continuous cropping obstacle.
Detailed description of the invention
The microbial soil conditioner of the controlled Sorghum vulgare Pers. continuous cropping obstacle that the present invention provides, for the fermentation liquid of bacillus amyloliquefaciens Bacillus amyloliquefaciens sp.Pb-4.
Bacillus amyloliquefaciens used in the present invention (Bacillus amyloliquefaciens sp.) Pb-4 bacterial strain is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center；Address: China, Beijing, postcode: 100101, deposit number is CGMCC N0.7155, and preservation date is on January 18th, 2013；
Bacillus amyloliquefaciens viable count about 2～5 × 10 in described fermentation liquid9cfu/ml.The microbial soil conditioner of described controlled Sorghum vulgare Pers. continuous cropping obstacle, the soil for Sorghum vulgare Pers. continuous cropping obstacle regulates and controls.
One, soil conditioner application process on continuous cropping Sorghum vulgare Pers. land for growing field crops in present embodiment
Continuous cropping Sorghum vulgare Pers. land for growing field crops when using, first will contain bacillus amyloliquefaciens viable count about 2～5 × 109The fermentation liquid of cfu/ml dilutes 50～150 times, makes the diluent of microbial soil conditioner.Then Sorghum vulgare Pers. sowing row or its root region soil it are applied to, for the control of Sorghum vulgare Pers. continuous cropping obstacle.
For Sorghum vulgare Pers. at plantation and growth period, the concrete application process of microbial soil conditioner diluent is:
(1), before sowing, the diluent of microbial soil conditioner is loaded in aerosol apparatus or fertilizer applicator behind whole ground, be applied to Sorghum vulgare Pers. sowing row.
(2), emerge laggard Seedling in the ranks, microbial soil conditioner diluent is carried out pouring root and uses by the different growing (seedling stage, jointing stage, Honoka phase) Sorghum vulgare Pers., or microbial soil conditioner is applied field with irrigation water, act on Sorghum vulgare Pers. root region soil.
Using, every mu of Sorghum vulgare Pers. cultivated area uses microbial soil conditioner diluent 100～300L every time.
Two, the application effect of the microbial soil conditioner of Sorghum vulgare Pers. continuous cropping obstacle can be controlled
1, test for potted plant continuous cropping Sorghum vulgare Pers.
Experimental cultivar: Sorghum vulgare Pers. Shanxi miscellaneous 34
Test site: Innovation Base greenhouse, academy of agricultural sciences of Shanxi Province
For examination soil: pick up from 5 years soil of proving ground, Dongyang, academy of agricultural sciences of Shanxi Province Sorghum vulgare Pers. continuous cropping, 10 years soil of proving ground Sorghum vulgare Pers. continuous cropping is improved literature by academy of agricultural sciences of Shanxi Province, 0～30cm soil layer.
For examination vessel: ￠ 21x18cm plastic tub, the hole of basin subdrilling ￠ 1.5cm, at the bottom of basin, add the high pallet of 2.5cm.Every basin dress soil 4kg.
For examination fertilizer: N fertilizer 0.2g/kg, P fertilizer 0.15g/kg, K fertilizer 0.15g/kg.
For examination microbial soil conditioner: bacillus amyloliquefaciens fermentation liquid, diluting 50-150 times, every basin adds diluent 1L, makes microbial inoculum bacterium number in basin soil reach 2～5 × 107cuf/g。
Test processes: 1. 5 years continuous cropping soil 2. 5 years continuous cropping soil+microbial soil conditioner 3. 10 years continuous cropping soil 4. 10 years continuous cropping soil+microbial soil conditioners, 4 process, repeats for 6 times, the strain of every basin 3.
Test method:
(1) potting soil dress basin is at 3/4, before Bo Zhong, microbial soil conditioner diluent is uniformly watered at soil surface, after microbial soil conditioner diluent partially absorbs, sow, on seed, after planting gently cover one layer of thin soil, for the sprouting of beneficially seed, can be at basin overlying thin film；
(2) emerging laggard Seedling in the ranks, microbial soil conditioner diluent is carried out pouring root and uses by the different growing (seedling stage, jointing stage, Honoka phase) Sorghum vulgare Pers.；
In greenhouse, ensure that Sorghum vulgare Pers. growing environment is in suitable condition, unified fertilising before sowing, after planting carry out identical management to potted plant, moisture, illumination, temperature is made all to keep consistent, and need regimen condition according to plant, quantitatively feed moisture, be unified in basin chassis the 200ml that waters every time, water penetrates in soil by the aperture at the bottom of basin, does not does in keeping basin；
Investigation and analysis: plant 60 days " Invest, Then Investigate " plants stems slightly, plant height, the upperground part biomass；Soil conditioner controls the effect of Sorghum vulgare Pers. continuous cropping obstacle and is shown in Table 1.Investigation finds, microbial soil conditioner has significant growth promoting function to Sorghum vulgare Pers. growth, can effectively control Sorghum vulgare Pers. continuous cropping obstacle.
The impact on Sorghum vulgare Pers. biological characteristics of table 1 microbial soil conditioner
2, test for land for growing field crops continuous cropping Sorghum vulgare Pers.
Experimental cultivar: Sorghum vulgare Pers. Shanxi miscellaneous 34
Test site: proving ground, Dongyang, academy of agricultural sciences of Shanxi Province
Result of the test: investigation plant pustulation period stem is thick, plant height, the number of blade, leaf area, microbial soil conditioner controls the effect of Sorghum vulgare Pers. continuous cropping obstacle and is shown in Table 2；Use microbial soil conditioner and the impact of Output of Sorghum is shown in Table 3.Result of the test shows, microbial soil conditioner has significant growth promoting function to Sorghum vulgare Pers., can improve yield, can effectively control Sorghum vulgare Pers. continuous cropping obstacle.
The impact on Sorghum vulgare Pers. biological characteristics of table 2 microbial soil conditioner
The impact on Output of Sorghum of table 3 microbial soil conditioner
Claims (7)
1. can control a microbial soil conditioner for Sorghum vulgare Pers. continuous cropping obstacle, it is characterized in that: this microorganism soil
Earth conditioner is the fermentation of bacillus amyloliquefaciens Bacillus amyloliquefaciens sp.Pb-4
Liquid.
The microbial soil conditioner of Sorghum vulgare Pers. continuous cropping obstacle, its feature can be controlled the most as claimed in claim 1
It is: the bacillus amyloliquefaciens viable count about 2～5 × 10 in described fermentation liquid9cfu/ml。
The microbial soil conditioner of Sorghum vulgare Pers. continuous cropping obstacle, its feature can be controlled the most as claimed in claim 1
It is: the soil for Sorghum vulgare Pers. continuous cropping obstacle regulates and controls.
The microbial soil conditioner side of using of Sorghum vulgare Pers. continuous cropping obstacle can be controlled the most as claimed in claim 1
Method, is characterized in that: the fermentation liquid of bacillus amyloliquefaciens Pb-4 is diluted 50～150 times, makes microorganism
Soil conditioner diluent, and be applied in the soil in Sorghum vulgare Pers. sowing Hang Huoqigen district.
The microbial soil conditioner side of using of Sorghum vulgare Pers. continuous cropping obstacle can be controlled the most as claimed in claim 4
Method, is characterized in that: described is applied to Sorghum vulgare Pers. sowing row or its root region soil, for Sorghum vulgare Pers. continuous cropping obstacle
Control, be before sowing, microbial soil conditioner diluent to be loaded in aerosol apparatus or fertilizer applicator behind whole ground,
It is applied to Sorghum vulgare Pers. sowing row.
The microbial soil conditioner side of using of Sorghum vulgare Pers. continuous cropping obstacle can be controlled the most as claimed in claim 4
Method, is characterized in that: described is applied to Sorghum vulgare Pers. sowing row or its root region soil, for Sorghum vulgare Pers. continuous cropping obstacle
Control, be the different growing Sorghum vulgare Pers., microbial soil conditioner diluent is carried out pouring root and uses or incite somebody to action
Microbial soil conditioner applies field with irrigation water, acts on Sorghum vulgare Pers. root region soil.
7. the microbial soil conditioner of the controlled Sorghum vulgare Pers. continuous cropping obstacle as described in claim 5 or 6 is executed
By method, it is characterized in that: use, every mu of Sorghum vulgare Pers. cultivated area uses microbial soil conditioner dilution every time
Liquid 100～300L.
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| CN201510875664.7A CN105820820A (en) | 2015-12-02 | 2015-12-02 | Microbial soil conditioner capable of controlling successive cropping obstacles of sorghum, and purpose and application method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105851062A (en) * | 2016-04-26 | 2016-08-17 | 山西省农业科学院农业环境与资源研究所 | Microorganism growth-promoting disease-preventing conditioning agent as well as use and application method |
| CN109644654A (en) * | 2017-10-12 | 2019-04-19 | 中国科学院南京土壤研究所 | A kind of method of prevention and control Xiangsha dasheen continuous cropping epidemic disease |
-
2015
- 2015-12-02 CN CN201510875664.7A patent/CN105820820A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105851062A (en) * | 2016-04-26 | 2016-08-17 | 山西省农业科学院农业环境与资源研究所 | Microorganism growth-promoting disease-preventing conditioning agent as well as use and application method |
| CN109644654A (en) * | 2017-10-12 | 2019-04-19 | 中国科学院南京土壤研究所 | A kind of method of prevention and control Xiangsha dasheen continuous cropping epidemic disease |
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