CN105831137A - Insecticidal composition containing destruxins and diflubenzuron, and preparation method and application thereof - Google Patents

Insecticidal composition containing destruxins and diflubenzuron, and preparation method and application thereof Download PDF

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CN105831137A
CN105831137A CN201610196613.6A CN201610196613A CN105831137A CN 105831137 A CN105831137 A CN 105831137A CN 201610196613 A CN201610196613 A CN 201610196613A CN 105831137 A CN105831137 A CN 105831137A
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agent
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grams
destruxins
ichlorbenzuron
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CN105831137B (en
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邵振芳
王泽清
陈建海
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Guangdong Xinjingxiang Bioengineering Co Ltd
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Guangdong Xinjingxiang Bioengineering Co Ltd
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
    • A01N47/28—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N<
    • A01N47/34—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N< containing the groups, e.g. biuret; Thio analogues thereof; Urea-aldehyde condensation products
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom

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  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Agronomy & Crop Science (AREA)
  • Plant Pathology (AREA)
  • Dentistry (AREA)
  • Wood Science & Technology (AREA)
  • Environmental Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Mycology (AREA)
  • Virology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention relates to an insecticidal composition with synergistic effect. The basic active components of the insecticidal composition are destruxins and diflubenzuron which are mixed according to a ratio of 1-9 g: 1-9 g. Adjuvants and excipients are added into the active components for preparation of a wettable powder, a suspending agent or an emulsion in water. The insecticidal composition can be applied to prevention and treatment of diamondback moth, prodenia litura, Lithocolletis ringoniella, grubs, wireworms, Trialeurodes vaporariorum, Liriomyza sativae, root maggots and Bradysia odoriphaga, preferably to prevention and treatment of lepidoptera insects like diamondback moth and prodenia litura on cruciferous vegetable, and has the advantages of stable efficacy, fast action, long action period, safety, environmental protection, etc.

Description

A kind of Pesticidal combination containing destruxins and d ichlorbenzuron and its preparation method and application
Technical field
The invention belongs to technical field of pesticide, be specifically related to a kind of containing destruxins and the Pesticidal combination of d ichlorbenzuron and preparation side Method and application.
Background technology
Destruxins (destruxin) is also known as destruxin or destroys element, the ring being made up of 5 aminoacid and 1 hydroxy acid Contracting carboxylic peptide toxin, a kind of secondary metabolite produced in metabolic process for green muscardine fungus (Pedras, et al., 2002), to squama wing 20 various pests such as mesh, Homoptera, Diptera, Isoptera, Orthoptera, coleoptera have action of contace poison (Strasser et al., 2000；Pedras,et al.,2002；Hu Qiongbo etc., 2007), stomach poison function (Brousseau et al., 1996) and refusing to eat are made With (Amiri et al., 1999；Thomsen et al., 2000)；Destruxins and some entomogenous fungi or commodity Mixture Use of Insecticides There is potentiation, can significantly improve Bemisia tabaci, twill night as mixed with beauveria bassiana, Paecilomyces javanicus, green muscardine fungus etc. The preventive effect of moth, Holotrichia diomphalia Bates etc. (Hu, et al., 2007；Hu Qiongbo etc., 2008)；Mixed with Bt, avilamycin, imidacloprid etc. also Can significantly improve preventive effect to choristoneura fumigerana, diamondback moth and Bemisia tabaci (Brousseua, et al., 1998；Hu, et al., 2009), And have efficiently, shelf life length, be susceptible to the features such as environmental influence, prevention effect are stable.
D ichlorbenzuron belongs to benzoyl area kind Chitin synthesis inhibitor, for insect growth regulators, it is possible to suppression insect chitin Synthesis thus cause insecticide normally not cast off a skin and dead.Affect the DNA in the respiratory metabolism of ovum and embryo development procedure and egg White matter metabolism, shows as good insecticidal activity to abnormal insecticide, particularly lepidopterous larvae.Not yet it is found to have relevant at present Destruxins and the compounded pesticides of d ichlorbenzuron.
Summary of the invention
It is an object of the invention to overcome the technical deficiency of existing insect hormone, it is provided that a kind of new and effective Pesticidal combination, The application of destruxins d ichlorbenzuron Pesticidal combination can reduce environmental pollution, reduce pesticide residues, delays pest resistance, subtracts Few Pesticide use amount.
The present invention is achieved through the following technical solutions:
A kind of Pesticidal combination of the present invention, containing destruxins and d ichlorbenzuron, described destruxins and described d ichlorbenzuron are by as follows Ratio mixes: described destruxins is (9-1) with the grams ratio of described d ichlorbenzuron: (1-9)
Preferably, the grams of described destruxins and described d ichlorbenzuron is than for 3:7,2:8,1:9.
Preferably, described compositions is made up of destruxins, d ichlorbenzuron and adjuvant.
The present invention, with destruxins and d ichlorbenzuron as host, adds adjuvant and can be processed into wettable powder, suspending agent, aqueous emulsion etc. Preparation.Described adjuvant changes because the dosage form of processing is different.
Described destruxins is the pure substance containing destruxins A, B, C, D, E or mixture, and described d ichlorbenzuron is for containing going out children The pure substance of urea or mixture.
Preferably, described adjuvant is wetting agent, dispersant, stabilizer and carrier, and each component of described adjuvant accounts for described parasite killing group The percent of the gross weight of compound is respectively as follows: wetting agent and accounts for 5-25%, and dispersant accounts for 4-15%, and stabilizer accounts for 0%-5%, carrier Complement to 100%；Described wetting agent is dodecylbenzene sodium sulfonate, the withered powder of benzene, spaonin powder, SOPA 235, detergent, draws Open one or more in powder, agriculture breast No. 2000 series, span 20, polysorbate60s；Described dispersant is NNO, MF, wood Quality sodium sulfonate, calcium lignosulfonate, polyoxyethylene polyoxypropylene base ether block copolymers, alkylphenol-polyethenoxy base phosphate ester In one or more.Described stabilizer is butylated hydroxyarisol, 2,6 ditertiary butyl p cresol, gallic acid third One or more in ester and tert-butylhydroquinone；Described carrier is Kaolin, white carbon, attapulgite, kieselguhr and swollen One or more in profit soil, precipitated calcium carbonate.
Above-mentioned adjuvant is processed into wettable powder, destruxins, d ichlorbenzuron, wetting agent, dispersant, carrier, stabilizer is mixed Close, mix homogeneously in premixing tank, after jet mill is pulverized, prepare micropowder.
Preferably, described adjuvant is thickening agent, emulsifying agent, co-emulsifier, stabilizer, antifreeze and solvent, described adjuvant The percent of the gross weight that each component accounts for described Pesticidal combination is respectively as follows: emulsifying agent and accounts for 5-20%, and co-emulsifier accounts for 5-10%, Thickening agent accounts for 0.5%-5%, and stabilizer accounts for 0.5%-5%, and antifreezing agent accounts for 0.5%-5%, solvent 10%-90%, and deionized water is supplied. Described emulsifying agent is ethylene oxide-propylene oxide block copolymer, alkyl polyglycol ether, alkyl phenyl polyglycol ether, polyoxy One or more in ethylene sorbitan ester, sulfonate, sulfate, lignosulfonates, phosphate ester；Described emulsifying altogether Agent is one or more in butanol, isobutanol, dodecanol, tetradecanol；Described thickening agent be xanthan gum, sodium alginate, One or more in arabic gum, bentonite, white carbon；Described stabilizer be butylated hydroxyarisol, 2,6-bis- One or more in Butylated Hydroxytoluene, propylgallate and tert-butylhydroquinone；Described antifreezing agent is ethylene glycol, urine One or more in element, glycerol, propylene glycol.Described solvent is one or more in alcohols, esters, arene.
Above-mentioned adjuvant is processed into aqueous emulsion, d ichlorbenzuron, destruxins is dissolved in proportion in solvent, by emulsifying agent, co-emulsifier, Stabilizer, antifreeze, thickening agent add in d ichlorbenzuron, destruxins mixed solution in the ratio of formula, after stirring It is slowly added to deionized water, stirs.
Preferably, the dosage form of described compositions is suspending agent, and the grams of described destruxins and described d ichlorbenzuron is than for 2:8.
Preferably, described adjuvant is wetting agent, dispersant, thickening agent, defoamer, thixotropic agent, preservative, stabilizer, prevents Freeze agent；The percent of the gross weight that each component of described adjuvant accounts for described Pesticidal combination is respectively as follows: wetting agent and accounts for 1-15%, point Powder accounts for 1-15%, and thickening agent accounts for 0.1-3%, and defoamer accounts for 0.5-5%, and thixotropic agent accounts for 0.5%-5%, and preservative accounts for 0.1%-1%, Stabilizer accounts for 0.1%-2%, antifreezing agent 0.1%-5%, and deionized water complements to 100%；Described wetting agent is K12, NPE, Ooze T, alkyl phenol (or fatty alcohol) polyoxyethylene poly-oxygen propylene aether and sulfate (or phosphate ester) thereof, polyether-modified poly-organic In siloxanes, sodium lauryl sulphate, fatty acid sulfates, fatty alcohol-polyoxyethylene ether, alkyl phenolic group polyoxyethylene ether One or more；Described dispersant is lignin salt, NNO, MF, BX, polycarboxylate, the contracting of alkylphenol-polyethenoxy formaldehyde One or more in compound sulfate, phosphate, morwet EFW, D-425；Described thickening agent be xanthan gum, CMC, One or more in PVA, sodium alginate, arabic gum, bentonite, white carbon, starch；Described defoamer for bubble enemy, One or more in organosilicon breast, C4-8 fatty alcohol, tributyl phosphate, machinery oil, silicone；Described thixotropic agent is silicic acid Magnalium；Described stabilizer is butylated hydroxyarisol, 2,6 ditertiary butyl p cresol, propylgallate and tertiary butyl One or more in hydroquinone；Described antifreezing agent is one or more in ethylene glycol, carbamide, glycerol, propylene glycol.
Above-mentioned adjuvant is processed into suspending agent, weigh destruxins, d ichlorbenzuron, wetting agent, dispersant, defoamer, thixotropic agent, Preservative, stabilizer, antifreezing agent proportionally mix through high shear dispersion, vertical grinding machine sand milling, after particle diameter reaches standard, It is proportionally added into thickening agent, stirs.
Preferably, the grams of the grams of described destruxins and described d ichlorbenzuron is than for 1:9.
Above-mentioned Pesticidal combination can be applicable to prevent and treat diamondback moth, Prodenia litura, apple leaf miner, Holotrichia diomphalia Bates, wireworm, trialeurodes vaporariorum, In liriomyza bryoniae, root maggot, Bradysia odoriphaga, preferably prevent and treat the lepidopteran insects such as brassicaceous vegetable diamondback moth, Prodenia litura. Application method is spraying.Formulation rate is different with the condition such as soil, crop true through simple experiment for age according to worm kind, insect density, worm Fixed.
Having obvious potentiation in the Pesticidal combination of the present invention, compared with single dose pesticide, insecticidal toxicity significantly improves, speed Effect property is good；The usage amount of pesticide reduces, and environmental pollution reduces；The present invention reduces the usage amount of destruxins, reduces production cost； Two kinds of diverse farm chemical ingredients of mechanism of action are compounded, adds target site, make insecticide be not likely to produce Drug resistance；Multiple Compositions after joining all has preferable prevention effect to the ovum of insect, larva, pupa, adult.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is further detailed, but the invention is not limited in this.In embodiment Percentage ratio refers both to percentage by weight, and described destruxins refers both to effective dose, and the amount of described d ichlorbenzuron is effective dose, actual preparation Time convert according to former medicine content used, the amount of deviation is adjusted by filling adding amount.
The embodiment of the present invention is the method using indoor virulence test and field test to combine.
Embodiment 1: destruxins d ichlorbenzuron wettable powder (grams of described destruxins and described d ichlorbenzuron than for 1:9)
Weigh destruxins 20 grams, d ichlorbenzuron 180 grams, wetting agent dodecylbenzene sodium sulfonate 50 grams, spaonin powder 5 gram, point Powder NNO 10 grams, alkylphenol-polyethenoxy base phosphate ester 30 grams, tert-butylhydroquinone 6 grams, surplus is kieselguhr conduct Carrier.
By described d ichlorbenzuron, described destruxins, described wetting agent, described dispersant, described stabilizer and described carrier in advance Mix homogeneously in mixed tank, prepares micropowder after jet mill is pulverized, obtains wettable powder Pesticidal combination.
In another embodiment, Pesticidal combination be formulated as weighing destruxins 20 grams, d ichlorbenzuron 180 grams, wetting agent benzene 25 grams of withered powder, spaonin powder 25 grams, Dispersant MF 40 grams, surplus is that Kaolin, white carbon are supplied gross weight as carrier and be 1000 grams.
In another embodiment, Pesticidal combination be formulated as weighing destruxins 20 grams, d ichlorbenzuron 180 grams, wetting agent SOPA 235 weight is 50 grams, detergent 50 grams, pull open 50 grams of powder, agriculture No. 2000 series of breast 100 grams, dispersant polyoxy Ethylene polyoxypropylene base ether block copolymers 150 grams, stabilizer 2,6 ditertiary butyl p cresol 50 grams, surplus is attapulgite Supplying gross weight with bentonite as carrier is 1000 grams.
In another embodiment, Pesticidal combination be formulated as weighing destruxins 20 grams, d ichlorbenzuron 180 grams, wetting agent department Each 100 grams of dish 20, polysorbate60, each 50 grams of dispersant sodium lignin sulfonate, calcium lignosulfonate, the stabilizer tert-butyl group is to hydroxyl Each 10 grams of base methoxybenzene, propylgallate, surplus is that to supply gross weight as carrier be 1000 grams to precipitated calcium carbonate.
Embodiment 2: destruxins Chlorbenzuron deflocculant (grams of described destruxins and described d ichlorbenzuron than for 2:8).
Weigh destruxins 20 grams, d ichlorbenzuron 80 grams, wetting agent sodium lauryl sulphate 20 grams, aliphatic alcohol polyethenoxy polyoxy 10 grams of propylene ether, Dispersant MF 10 grams, morwet10 gram, thickening agent xanthan gum 50 grams, defoamer silicone 20 grams, Thixotropic agent aluminium-magnesium silicate 15 grams, stabilizer butylated hydroxyarisol 5 grams, tert-butylhydroquinone 5 grams, preservative benzene first 5 grams of sodium of acid, antifreeze glycol 5 grams, surplus is deionized water.
By described d ichlorbenzuron, described wetting agent, described dispersant, described stabilizer, described defoamer, described thixotropic agent with go Ionized water is sequentially added in mixing kettle, stirs, and enters sand mill and carry out sand milling after high shear dispersion still is sheared, and sand milling is extremely After 90% particle diameter is less than 5 micronsAdd xanthan gum to stir, obtain suspending agent disinfestation composition.
In another embodiment, Pesticidal combination be formulated as weigh destruxins 20 grams, d ichlorbenzuron 80 grams, wetting agent K12 Weight 1 gram, NPE weight 1 gram, ooze totally 7 grams of T weight 1 gram, alkylphenol-polyethenoxy polyethenoxy ether and sulfate thereof, Dispersant lignin salt 10 grams, thickening agent CMC0.3 gram, PVA0.3 gram, sodium alginate 0.4 gram, defoamer bubble enemy 2 grams, Organosilicon breast 3 grams, thixotropic agent aluminium-magnesium silicate 5 grams, preservative sodium benzoate 1 gram, stabilizer 2,6 ditertiary butyl p cresol 1 gram, 1 gram of antifreezing agent carbamide, surplus is that deionized water complements to 1000 grams.
In another embodiment, Pesticidal combination be formulated as weigh destruxins 20 grams, d ichlorbenzuron 80 grams, wetting agent fat Alcohol polyoxyethylene poly-oxygen propylene aether and phosphate ester 150 grams thereof, dispersant NNO30 gram, BX30 gram, polycarboxylate 30 grams, 60 grams of alkylphenol-polyethenoxy formaldehyde condensation products sulfate, thickening agent arabic gum 10 grams, bentonite 10 grams, white carbon 10 Gram, defoamer C4-8 fatty alcohol 50 grams, thixotropic agent aluminium-magnesium silicate 50 grams, stabilizer propylgallate 20 grams, preservative Sodium benzoate 10 grams, antifreezing agent glycerol 50 grams, surplus is that deionized water complements to 1000 grams.
In another embodiment, Pesticidal combination be formulated as weigh destruxins 20 grams, d ichlorbenzuron 80 grams, wetting agent polyethers Modified polyorganosiloxanes, each 25 grams of fatty acid sulfates, fatty alcohol-polyoxyethylene ether, alkyl phenolic group polyoxyethylene ether, Dispersant phosphate 40 grams, each 30 grams of EFW, D-425, thickening agent starch 20 grams, defoamer tributyl phosphate, machinery Each 15 grams of oil, thixotropic agent aluminium-magnesium silicate 20 grams, each 5 grams of stabilizer 2,6 ditertiary butyl p cresol, propylgallate, Preservative sodium benzoate 5 grams, antifreezing agent propylene glycol 20 grams, surplus is that deionized water complements to 1000 grams.
Embodiment 3: destruxins d ichlorbenzuron aqueous emulsion (grams of described destruxins grams and described d ichlorbenzuron than for 3:7)
Weigh destruxins 45 grams, d ichlorbenzuron 105 grams, emulsifying agent alkyl polyglycol ether 20 grams, lignosulfonates 30 grams Co-emulsifier isobutanol 50 grams, thickening agent xanthan gum 10 grams, stabilizer butylated hydroxyarisol 10 grams, antifreezing agent second two Alcohol 5 grams, solvent is that methanol accounts for 100 grams, and dimethylbenzene 200 grams, surplus is deionized water.
By described destruxins, described d ichlorbenzuron, methanol, xylene solvent, it is sequentially added in mixing kettle, stirs, treat After being completely dissolved add alkyl polyglycol ether, lignosulfonates, isobutanol, xanthan gum, butylated hydroxyarisol, Ethylene glycol, deionized water, high shear 1 hour, obtain aqueous emulsion Pesticidal combination.
In another embodiment, Pesticidal combination be formulated as destruxins 45 grams, d ichlorbenzuron 105 grams, emulsifying agent oxirane Each 25 grams of-propylene oxide block copolymer, alkyl phenyl polyglycol ether, 50 grams of co-emulsifier butanol, thickening agent sodium alginate 5 grams, stabilizer 2,6 ditertiary butyl p cresol 5 grams, 5 grams of antifreezing agent carbamide, solvent is that methanol accounts for 100 grams of surpluses for going Ionized water complements to 1000 grams.
In another embodiment, Pesticidal combination be formulated as destruxins 45 grams, d ichlorbenzuron 105 grams, emulsifier polyoxyethylene Totally 30 grams of sorbitan ester, sulfonate, sulfate, each 25 grams of co-emulsifier dodecanol, isobutanol, thickening agent I Each 5 grams of primary glue, bentonite, stabilizer propylgallate 10 grams, antifreezing agent glycerol 5 grams, solvent is that methanol accounts for 100 grams, Dimethylbenzene 200 grams, surplus is that deionized water complements to 1000 grams.
In another embodiment, Pesticidal combination be formulated as destruxins 45 grams, d ichlorbenzuron 105 grams, emulsifying agent phosphate ester 100 grams, co-emulsifier tetradecanol 80 grams, thickening agent white carbon 20 grams, stabilizer tert-butylhydroquinone 20 grams, antifreeze Agent propylene glycol 20 grams, solvent is that methanol accounts for 100 grams, dimethylbenzene 200 grams, and surplus is that deionized water complements to 1000 grams.
Embodiment 4: the present embodiment is the method using indoor virulence test and field test to combine
Test reagent: the former powder of destruxins 90%, benzoylphenyl urea original medicine 97%.
Subjects: diamondback moth 3 instar larvae Plutella xylostella (Linnaeus)
Test method: use leaf dipping method test.The compound method of medicament is with reference to pesticide toxicity evaluation method, respectively to Avermectin Element (A), d ichlorbenzuron (B), with preliminary survey LC50Centered by value, concentration respectively extends 2 gradient concentrations to two ends, sets 5 altogether Concentration for the treatment of, is processed as comparison with clear water.The cabbage leaves being not in contact with any medicament is soaked into the liquid 10s taking-up dry Rear defence enters in culture dish, and every culture dish accesses healthy growth, diamondback moth of the same size 3 instar larvae 20, every concentration weight Multiple 4 times, it is placed in calorstat (temperature 25 DEG C ± 1 DEG C), checks result after 48h, calculate mortality rate and corrected mortality, Carry out statistical analysis with SPSS data processing software, calculate the LC of each medicament50, evaluate the activity of reagent agent with this.
The co-toxicity coefficient (CTC value) of mixture is calculated by following equation:
A T I = S M &times; 100
In formula:
ATI-mixture actual measurement toxicity index；
The LC of S-standard pesticide agent50, unit is milligrams per liter；
The LC of M-mixture50, unit is milligrams per liter.
TTI=TIA × PA+TIB × PB
In formula:
TTI mixture theory toxicity index；
TIA A medicament toxicity index；
PA A medicament percentage composition in mixture, unit is percentage rate；
TIB B medicament toxicity index；
PB B medicament percentage composition in mixture, unit is percentage rate.
In formula:
CTC co-toxicity coefficient；
AT mixture actual measurement toxicity index；
TTI mixture theory toxicity index.
The co-toxicity coefficient (CTC) of built agent:
C T C = A T I T T I &times; 100
CTC >=120 show as potentiation；
CTC≤80 show as antagonism；
80 ＜ CTC ＜ 120 show as summation action.
Table 1 is the work of the different reagent agents of the Pesticidal combination of the destruxins (A) containing different proportion of the present invention and d ichlorbenzuron (B) Property (LC50), the testing result of co-toxicity coefficient (CTC).By table 1 result it can be seen that the matter of destruxins and d ichlorbenzuron Amount ratio is for (1-9): (9-1) all shows obvious potentiation, wherein with destruxins: d ichlorbenzuron (mass ratio)=2: 8 synergistic effects are the most obvious.
Table 1
A: destruxins B: d ichlorbenzuron
Embodiment 5: Field information is tested:
This experimental establishment is in Xi Qiao town, Fushan City, Guangdong Province, and test medicine is researched and developed by Guangdong new scene biological engineering company limited, carried Confession, comparison medicament 0.5% destruxins suspending agent (Agricultural University Of South China's offer), 20% Chlorbenzuron deflocculant (commercial).
In diamondback moth larvae occurance peak (based on 2～3 ages), Caulis et Folium Brassicae capitatae growth is in the lotus throne initial stage and carries out spraying medicine preventing and treating.Spray Medicine apparatus is (Super-Green)-16 type knapsack sprayer, and spray value is 45L/667m2, spray at cabbage plant leaf table In face and lobus cardiacus.
Efficacy survey method uses the method determining strain investigation.Dispenser Qian Mei community by 5 point samplings and with bamboo let labelling Caulis et Folium Brassicae capitatae, Carefully investigate and record the population number on every dish as insect population radix before medicine, 1,3,7,14 days investigation fixed points after dispenser In survival borer population, calculate Revision insect recluced rate and prevention effect by following equation, and with Deng Kenshi new multipole difference method of inspection (DMRT Method) carry out statistical analysis, compare the level of difference of prevention effect between each process.The results are shown in Table 2, by seen from this table this Bright compositions reaches more than 80% for preventive effect after dose on probation spraying 1d, and after showing preferable quick-acting, medicine, 14d preventive effect can To reach more than 75%, at least 14 days lasting periods.The present composition, while improve preventive effect, reduces dosage.
Table 2
Last institute is it should be noted that, above example is only in order to illustrate technical scheme rather than to scope Restriction, although the present invention being explained in detail with reference to preferred embodiment, it will be understood by those within the art that, can Technical scheme is modified or equivalent, without deviating from the spirit and scope of technical solution of the present invention.

Claims (10)

1. one kind contains destruxins and the Pesticidal combination of d ichlorbenzuron, it is characterised in that described Pesticidal combination contains destruxins And d ichlorbenzuron, described destruxins is mixed in following ratio with described d ichlorbenzuron: the grams of described destruxins and described d ichlorbenzuron Grams than for (1-9): (9-1).
Pesticidal combination the most according to claim 1, it is characterised in that described compositions is by destruxins, d ichlorbenzuron Forming with adjuvant, described destruxins is the pure substance containing destruxins A, B, C, D, E or mixture, described d ichlorbenzuron For the pure substance containing d ichlorbenzuron or mixture.
Pesticidal combination the most according to claim 2, it is characterised in that described adjuvant is wetting agent, dispersant, stablizes Agent and carrier, the percent of the gross weight that the weight of each component of described adjuvant accounts for described Pesticidal combination is respectively as follows:
Described wetting agent accounts for 5-25%；
Described dispersant accounts for 4-15%；
Described stabilizer accounts for 0%-5%；
Described carrier complements to 100%；
Described wetting agent is dodecylbenzene sodium sulfonate, the withered powder of benzene, spaonin powder, SOPA235, detergent, pull open powder, agriculture breast One or more in No. 2000 series, span 20, polysorbate60s；
Described dispersant is NNO, MF, sodium lignin sulfonate, calcium lignosulfonate, polyoxyethylene polyoxypropylene base ether block One or more in copolymer, alkylphenol-polyethenoxy base phosphate ester；
Described stabilizer is butylated hydroxyarisol, 2,6 ditertiary butyl p cresol, propylgallate and tertiary butyl pair One or more in Benzodiazepines；
Described carrier is one or more in Kaolin, white carbon, attapulgite, kieselguhr and bentonite, precipitated calcium carbonate.
4. the preparation method of the Pesticidal combination described in claim 3, it is characterised in that by described destruxins, described in go out children Urea, described wetting agent, described dispersant, described stabilizer, described carrier mix, and mix homogeneously in premixing tank, through air-flow Pulverizer prepares micropowder after pulverizing.
Pesticidal combination the most according to claim 2, it is characterised in that described adjuvant is thickening agent, emulsifying agent, is total to Emulsifying agent, stabilizer, antifreezing agent and solvent, the weight of each component of described adjuvant accounts for the hundred of the gross weight of described Pesticidal combination Mark is respectively as follows:
Described thickening agent accounts for 0.5%-5%；
Described emulsifying agent accounts for 5-20%；
Described co-emulsifier accounts for 5-10%；
Described stabilizer accounts for 0.5%-5%；
Described antifreezing agent accounts for 0.5%-5%；
Described solvent 10%-90%；
Described deionized water supplies 100%；
Described thickening agent is one or more in xanthan gum, sodium alginate, arabic gum, bentonite, white carbon；
Described emulsifying agent be ethylene oxide-propylene oxide block copolymer, alkyl polyglycol ether, alkyl phenyl polyglycol ether, One or more in polyoxyethylene sorbitol acid anhydride ester, sulfonate, sulfate, lignosulfonates, phosphate ester；
Described co-emulsifier is one or more in butanol, isobutanol, dodecanol, tetradecanol；
Described stabilizer is butylated hydroxyarisol, 2,6 ditertiary butyl p cresol, propylgallate and tertiary butyl pair One or more in Benzodiazepines；
Described antifreezing agent is one or more in ethylene glycol, carbamide, glycerol, propylene glycol；
Described solvent is one or more in alcohols, esters, arene.
6. the preparation method of the Pesticidal combination described in claim 5, it is characterised in that by described d ichlorbenzuron, described green muscardine fungus Element is dissolved in described solvent in proportion, by described emulsifying agent, described thickening agent, described co-emulsifier, described stabilizer, described Antifreeze is added in described d ichlorbenzuron and described destruxins mixed solution in the ratio of formula, is slowly added to after stirring Deionized water, stirs.
Pesticidal combination the most according to claim 2, it is characterised in that described adjuvant is wetting agent, dispersant, thickening Agent, defoamer, thixotropic agent, preservative, stabilizer, antifreezing agent；The weight of each component of described adjuvant accounts for described Insecticidal combinations The percent of the gross weight of thing is respectively as follows:
Described wetting agent accounts for 1-15%；
Described dispersant accounts for 1-15%；
Described thickening agent accounts for 0.1-3%；
Described defoamer accounts for 0.5-5%；
Described thixotropic agent accounts for 0.5%-5%；
Described preservative accounts for 0.1%-1%；
Described stabilizer accounts for 0.1%-2%；
Described antifreezing agent 0.1%-5%；
Deionized water complements to 100%；
Described wetting agent is K12, NPE, ooze T, alkyl phenol (or fatty alcohol) polyoxyethylene poly-oxygen propylene aether and sulfate thereof (or phosphate ester), polyether-modified polysiloxane, sodium lauryl sulphate, fatty acid sulfates, aliphatic alcohol polyethenoxy One or more in ether, alkyl phenolic group polyoxyethylene ether；
Described dispersant is lignin salt, NNO, MF, BX, polycarboxylate, alkylphenol-polyethenoxy formaldehyde condensation products sulphuric acid One or more in salt, phosphate, morwet EFW, D-425；
Described thickening agent is in xanthan gum, CMC, PVA, sodium alginate, arabic gum, bentonite, white carbon, starch One or more；
Described defoamer be bubble enemy, organosilicon breast, C4-8 fatty alcohol, tributyl phosphate, machinery oil, silicone in one or Multiple；
Described thixotropic agent is aluminium-magnesium silicate；Described stabilizer be butylated hydroxyarisol, 2,6 ditertiary butyl p cresol, One or more in propylgallate and tert-butylhydroquinone；
Described stabilizer is butylated hydroxyarisol, 2,6 ditertiary butyl p cresol, propylgallate and tertiary butyl pair One or more in Benzodiazepines；
Described antifreezing agent is one or more in ethylene glycol, carbamide, glycerol, propylene glycol.
8. the preparation method of the Pesticidal combination described in claim 7, it is characterised in that by described destruxins, described in go out children Urea, described wetting agent, described dispersant, described defoamer, described thixotropic agent, described preservative, described stabilizer and described Antifreezing agent proportionally mixes through high shear dispersion, vertical grinding machine sand milling, after sand milling to 90% particle diameter is less than 5 microns, by than Example adds described thickening agent, stirs.
Pesticidal combination the most according to claim 7, it is characterised in that the dosage form of described compositions is suspending agent, described The grams of the grams of destruxins and described d ichlorbenzuron is than for 1:9.
10. the Pesticidal combination that one of claim 1-9 is described is preventing and treating scale insect diamondback moth, white lead at above-mentioned Pesticidal combination Louse Prodenia litura, thrips apple leaf miner, Holotrichia diomphalia Bates, wireworm, citrus fruit fly trialeurodes vaporariorum, liriomyza bryoniae, root maggot, fragrant-flowered garlic maggot and Folium Allii tuberosi The application of Bradysia fungus gnat.
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