CN105816521A - Bothriospermum chinense (Turcz.) bunge extract for treating urinary tract infection - Google Patents
Bothriospermum chinense (Turcz.) bunge extract for treating urinary tract infection Download PDFInfo
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- CN105816521A CN105816521A CN201610385974.5A CN201610385974A CN105816521A CN 105816521 A CN105816521 A CN 105816521A CN 201610385974 A CN201610385974 A CN 201610385974A CN 105816521 A CN105816521 A CN 105816521A
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- Prior art keywords
- urinary tract
- tract infection
- bufo siccus
- extract
- thick paste
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
- A61K36/537—Salvia (sage)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
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- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Medicines Containing Plant Substances (AREA)
- Medicinal Preparation (AREA)
Abstract
The invention relates to the technical field of medicine, in particular to bothriospermum chinense (Turcz.) bunge extract for treating urinary tract infection .When the extract is manufactured, bothriospermum chinense (Turcz.) bunge is crushed to 80 meshes, distilled water is added, treatment is conducted for 45 minutes under microwaves with the power being 85 W, an object stands still, upper clear liquid is taken, residues are reserved, the upper clear liquid is concentrated to thick paste with the water content being 8% under a rotary evaporator, the thick paste is subjected to vacuum drying and crushed to a substance A in a powder shape, ethyl alcohol with the concentration being 75% and being eight times the size of the residues is added to the residues, reflux extraction is conducted for 1 hour at the temperature of 80 DEG C, an extraction solution is concentrated to thick paste with the water content being 8% under the rotary evaporator, the thick paste is subjected to vacuum drying and crushed into a substance B in a powder shape, the powdery A and the powdery B are mixed, and the bothriospermum chinense (Turcz.) bunge extract for treating urinary tract infection is obtained .The bothriospermum chinense (Turcz.) bunge extract for treating urinary tract infection has the effects of clearing away heat and toxic materials, sterilizing and diminishing inflammation and relieving stranguria by dieresis, the effect on treatment of urinary tract infection is obvious, medicine application is safe, and no toxic or side effect exists.
Description
Technical field
The present invention relates to pharmaceutical technology field, be specifically related to a kind of Bufo siccus grass extract treating urinary tract infection.
Background technology
Bufo siccus grass is the plant that Lamiaceae Salvia belongs to, annual or biennial herb, and multi-branched is swung to thin pubescence, cool in nature, bitter in the mouth, pungent.From clinical practice, Bufo siccus grass extract is used for antitussive, treatment laryngopharynx swelling and pain.There are no at present with the report in terms of its extract for treating urinary tract infection.
Summary of the invention
It is an object of the invention to provide a kind of Bufo siccus grass extract treating urinary tract infection.
A kind of Bufo siccus grass extract treating urinary tract infection, prepares by the following method:
Bufo siccus grass meal is broken to 80 mesh, the distilled water adding Bufo siccus grass weight 10 times processes 45 minutes under the microwave that power is 85W, standing takes supernatant liquid, retain residue, being 0.098mpa by supernatant liquid in vacuum, the speed of rotation is 100rpm, and temperature is to be concentrated into, under the Rotary Evaporators of 72-75 DEG C, the thick paste that water content is 8%, thick paste is vacuum dried, is ground into powder thing A；
In residue, add the ethanol of residue volume 8 times 75%, reflux, extract, 1 hour at 80 DEG C, be 0.098mpa by extracting solution in vacuum, the speed of rotation is 100rpm, temperature is to be concentrated into, under the Rotary Evaporators of 65-68 DEG C, the thick paste that water content is 8%, is vacuum dried by thick paste, is ground into powder thing B；
Powder thing A and powder thing B is mixed to get the Bufo siccus grass extract for the treatment of urinary tract infection.
The Bufo siccus grass extract of described treatment urinary tract infection moors flavonoid glycoside 0.2mg/g containing protocatechuic acid 0.037mg/g, homoplantaginin 0.18mg/g, Buddhist nun.
The Bufo siccus grass extract of described treatment urinary tract infection is used for being prepared as tablet, granule, oral liquid, capsule as sole active ingredient, wherein, every tablet is containing described Bufo siccus grass extract 1.3g, often bag granule contains described Bufo siccus grass extract 1.3g, containing described Bufo siccus grass extract 1g in every 15ml oral liquid, containing described Bufo siccus grass extract 1.3g in every seed lac capsule.
The present invention treats the Bufo siccus grass extract of urinary tract infection, has effect of heat-clearing and toxic substances removing, inhibiting bacteria and diminishing inflammation, relieving stranguria by diuresis, and treatment urinary tract infection effect is obvious, and drug safety has no side effect.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention will be described,
Embodiment 1,
Bufo siccus grass meal is broken to 80 mesh, the distilled water adding Bufo siccus grass weight 10 times processes 45 minutes under the microwave that power is 85W, standing takes supernatant liquid, retain residue, being 0.098mpa by supernatant liquid in vacuum, the speed of rotation is 100rpm, and temperature is to be concentrated into, under the Rotary Evaporators of 72-75 DEG C, the thick paste that water content is 8%, thick paste is vacuum dried, is ground into powder thing A；
In residue, add the ethanol of residue volume 8 times 75%, reflux, extract, 1 hour at 80 DEG C, be 0.098mpa by extracting solution in vacuum, the speed of rotation is 100rpm, temperature is to be concentrated into, under the Rotary Evaporators of 65-68 DEG C, the thick paste that water content is 8%, is vacuum dried by thick paste, is ground into powder thing B；
Powder thing A and powder thing B is mixed to get the Bufo siccus grass extract for the treatment of urinary tract infection.
The Bufo siccus grass extract of described treatment urinary tract infection moors flavonoid glycoside 0.2mg/g containing protocatechuic acid 0.037mg/g, homoplantaginin 0.18mg/g, Buddhist nun.
The Bufo siccus grass extract of described treatment urinary tract infection is used for being prepared as tablet, granule, oral liquid, capsule as sole active ingredient, wherein, every tablet is containing described Bufo siccus grass extract 1.3g, often bag granule contains described Bufo siccus grass extract 1.3g, containing described Bufo siccus grass extract 1g in every 15ml oral liquid, containing described Bufo siccus grass extract 1.3g in every seed lac capsule.
Select 10-12 week old male rat 50, body weight 180-250g, it is randomly divided into A, B two groups, after rat is prohibited water 18 hours, with the pentobarbital sodium 40mg/kg of 2%, intraperitoneal injection of anesthesia, rat is fixed on dissection plate, sterilizes after abdominal part unhairing, cut open the belly, enter abdominal cavity, spill left side posterior abdominal wall, see clearly left side ureter, the angular pin being installed with silk thread is threaded a needle by outside the stomach wall respectively backward of both sides, ureter stage casing, exposing bladder, by 0.75ml/ Escherichia coli bacteria liquid, (in bacterium solution, escherichia coli bacterium number is 3-5x104/ ml) it is slowly injected into rat bladder, after having injected, silk thread two ends outside tension stomach wall, ligature ureter with suitable elasticity, cleaning abdominal cavity, layer-by-layer suture otch, recovers drinking-water and feeds, postoperative 20 hours, remove the Unilateral Ureteral Obstruction line outside stomach wall, make ureter reopen.
After open ureter, it is administered 2 every day, to A group rat every/each Bufo siccus grass extract to 1.3g treatment urinary tract infection, (preparation method is the Bufo siccus grass extract prepared every/every day of B group rat according to a conventional method to 1.3g, Bufo siccus grass is mixed by the weight ratio of 1:10 with water, heating and cooking 30 minutes, take boiling liquid and concentrate cream processed, final vacuum drying, is ground into powder thing).
Being administered latter 1 hour and observe two groups of rat two just symptoms of A, B, obtain, B group rat urine frequency number is significantly more than A group (P ＜ 0.01), after successive administration 3 times, the A group rat mental status recovers good, and two is the most normal, B group rat lethargy, slow movement, frequent micturition, dry stool.
After successive administration 2 days, put to death two groups of rats of A, B, extract two groups of rat bladder urines of A, B in an aseptic environment, it is inoculated on plating medium, after being placed in 37 DEG C of calorstats cultivation 24 hours, observes bacterial growth situation, as a result, A group has a large amount of bacterial growth without bacterial growth, B group.
Claims (3)
1. the Bufo siccus grass extract treating urinary tract infection, it is characterised in that this extract prepares by the following method:
Bufo siccus grass meal is broken to 80 mesh, the distilled water adding Bufo siccus grass weight 10 times processes 45 minutes under the microwave that power is 85W, standing takes supernatant liquid, retain residue, being 0.098mpa by supernatant liquid in vacuum, the speed of rotation is 100rpm, and temperature is to be concentrated into, under the Rotary Evaporators of 72-75 DEG C, the thick paste that water content is 8%, thick paste is vacuum dried, is ground into powder thing A；
In residue, add the ethanol of residue volume 8 times 75%, reflux, extract, 1 hour at 80 DEG C, be 0.098mpa by extracting solution in vacuum, the speed of rotation is 100rpm, temperature is to be concentrated into, under the Rotary Evaporators of 65-68 DEG C, the thick paste that water content is 8%, is vacuum dried by thick paste, is ground into powder thing B；
Powder thing A and powder thing B is mixed to get the Bufo siccus grass extract for the treatment of urinary tract infection.
A kind of Bufo siccus grass extract treating urinary tract infection the most according to claim 1, it is characterised in that
The Bufo siccus grass extract of described treatment urinary tract infection moors flavonoid glycoside 0.2mg/g containing protocatechuic acid 0.037mg/g, homoplantaginin 0.18mg/g, Buddhist nun.
A kind of Bufo siccus grass extract treating urinary tract infection the most according to claim 1, it is characterised in that
The Bufo siccus grass extract of described treatment urinary tract infection is used for being prepared as tablet, granule, oral liquid, capsule as sole active ingredient, wherein, every tablet is containing described Bufo siccus grass extract 1.3g, often bag granule contains described Bufo siccus grass extract 1.3g, containing described Bufo siccus grass extract 1g in every 15ml oral liquid, containing described Bufo siccus grass extract 1.3g in every seed lac capsule.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610385974.5A CN105816521A (en) | 2016-06-03 | 2016-06-03 | Bothriospermum chinense (Turcz.) bunge extract for treating urinary tract infection |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610385974.5A CN105816521A (en) | 2016-06-03 | 2016-06-03 | Bothriospermum chinense (Turcz.) bunge extract for treating urinary tract infection |
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| CN105816521A true CN105816521A (en) | 2016-08-03 |
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| CN201610385974.5A Pending CN105816521A (en) | 2016-06-03 | 2016-06-03 | Bothriospermum chinense (Turcz.) bunge extract for treating urinary tract infection |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106619836A (en) * | 2016-11-18 | 2017-05-10 | 安徽禾泉农庄生态农业有限公司 | Method for extracting active ingredient from all-grass of Bothriospermum chinense Bunge and applications of active ingredient |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104840603A (en) * | 2015-05-29 | 2015-08-19 | 重庆希尔安药业有限公司 | Belladonna extract and preparation method thereof |
-
2016
- 2016-06-03 CN CN201610385974.5A patent/CN105816521A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104840603A (en) * | 2015-05-29 | 2015-08-19 | 重庆希尔安药业有限公司 | Belladonna extract and preparation method thereof |
Non-Patent Citations (3)
| Title |
|---|
| 叶勇: "《制药工艺学》", 28 February 2014, 华南理工大学出版社 * |
| 周德生等: "《简明中药手册》", 31 January 2014, 山西科学技术出版社 * |
| 范文昌等: "《广东地产清热解毒药物大全》", 31 July 2011, 中医古籍出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106619836A (en) * | 2016-11-18 | 2017-05-10 | 安徽禾泉农庄生态农业有限公司 | Method for extracting active ingredient from all-grass of Bothriospermum chinense Bunge and applications of active ingredient |
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Application publication date: 20160803 |