CN104840603A - Belladonna extract and preparation method thereof - Google Patents
Belladonna extract and preparation method thereof Download PDFInfo
- Publication number
- CN104840603A CN104840603A CN201510287958.8A CN201510287958A CN104840603A CN 104840603 A CN104840603 A CN 104840603A CN 201510287958 A CN201510287958 A CN 201510287958A CN 104840603 A CN104840603 A CN 104840603A
- Authority
- CN
- China
- Prior art keywords
- preparation
- belladonna extract
- thick paste
- belladonna
- value
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The invention provides a preparation method of a belladonna extract. The preparation method comprises the following steps: (A) soaking coarse powder of belladonna herb in ethanol, percolating the coarse powder, filtering the percolate and carrying out evaporation concentration on the filtrate, thus obtaining first thick paste; (B) adding water to dilute residual substances after filtration, adjusting the PH value to acidity, then separating upper chlorophyll, collecting lower clear liquor, adjusting the PH value to alkalinity and concentrating the clear liquor, thus obtaining second thick paste; (C) mixing the first thick paste with the second thick paste, thus obtaining the belladonna extract. The content of atropine sulfate in the belladonna extract obtained by adopting the preparation method is between 9.8mg/ml and 10mg/ml. The belladonna extract has the advantages that the belladonna extract has good effects and high purity; all the indexes meet the corresponding requirements of the national formulary and even outclass the effects superior to the quality indexes stipulated in the formulary.
Description
Technical field
The present invention relates to medical art, in particular to a kind of belladonna extract and preparation method thereof.
Background technology
Semen daturae is a kind of Solanaceae herbaceous plant.Originate in the herbaceous plant for many years in West Europe, be transplanted to the ground such as north African, West Asia, North America afterwards, China also has to introduce cultivates.Original producton location is the in the shade sough in mountain region, is being rich in all living creatures in calcareous soil.The early spring germinates, and total length 40-50 centimetre, reaches as high as 5 meters.Root is cylindrical, diameter 5-15 millimeter, surperficial grayish brown, and tool indulges wrinkle; Old root is wooden, radicula frangibility, and section is smooth, and skin zone is narrow, canescence, and woody part is broad, brown color, and cambium layer ring grain is obvious, marrow hollow.The oblate cylindricality of stem, diameter 3-6 millimeter, surperficial yellow green, has thin vertical wrinkle and dredges rare choice refreshments shape hole skin, hollow, young stem hairiness.The many shrinkages of leaf are broken, and intact leaf ovum shape is oval, yellowish green to bottle green.Florescence can continue until summer, opens darkviolet flower, and calyx 5 splits, and corolla is bell.
Effective ingredient in Semen daturae is belladonna alkaloid, is mainly used in stomachache, the vomiting that gastritis and stomach spasm cause and diarrhoea that stomach spasm, duodenal ulcer, ureteral calculus etc. cause.
General great majority all adopt percolation to extract belladonna extract (Semen daturae extractum, Semen daturae fluid extract) for the extraction process of Semen daturae, a large amount of chlorophylls can be produced in process, about 1000 kilograms of Herba Belladonnaes produce the chlorophyll garbage of about 23 kilograms, common practices of the prior art is directly abandoned by these garbages, but this way not only also can contain a certain amount of effective ingredient in not environmental protection but also chlorophyll, directly abandon and can cause the wasting of resources, improve production cost virtually, both uneconomical also not environmentally.
In view of this, special proposition the present invention.
In prior art, general is all directly in the process of normally producing belladonna extract (i.e. Semen daturae fluid extract), remaining residual substances (main containing chlorophyll in residual substances) will be filtered directly abandon, but but ignore effective ingredient remaining in residual substances, namely required hyoscyami alkaloid, the present invention is just by a large amount of knowhows, find the value of residual substances inherence, therefrom extract effective ingredient, be incorporated in the thick paste normally produced and obtain, not only supplement output, can cost-savingly increase operation rate and expand economic benefit, and detect by each side the requirements that its product quality well meets " Chinese Pharmacopoeia ", on the indices that even its inherent index specifies far away from " Chinese Pharmacopoeia ", during embody rule, therapeutic effect is remarkable.
Wherein, Herba Belladonnae coarse powder first passes through normal production operation flow process, namely after soak with ethanol, percolation, filtration and evaporation and concentration operating procedure, obtains the first thick paste, and the order granularity of coarse powder is between 10-24 order.In course of normal operation, the selection of solvent is preferably selected chemical substance that is harmless and environmental protection, therefore ethanol is optimum selection, solvent is first adopted fully to flood, be conducive to the stripping of effective ingredient in subsequent extracted process like this, the time of dipping preferably at 48 hours because also unsuitable long for the consideration dip time of production cycle.The mass percent concentration of ethanol used preferably controls between 85-88%, and within the scope of this mass percent concentration, its extraction effect is better.
In addition, although percolation belongs to the routine operation in extract drugs process, but still there is certain specific requirement for different extract drugs in conjunction with himself character, therefore in the preparation process of belladonna extract of the present invention, the number of times of its percolation or conditional, preferably control between 2-3 time, and the rate controlled of each percolation is at 1-3ml/min, fully to extract effective ingredient wherein, it completely essence substance may not extracted if percolation speed is too fast, the purity of product can be had influence on.Also have, the percolate that percolation obtains for the first time is called liquid of just filtering, liquid of just filtering obtains rear general meeting decompression recycling ethanol under 60 DEG C of conditions, then let cool to room temperature, after filtering, evaporation and concentration is to thick paste, then is concentrated into thick paste, also decompression recycling ethanol under 60 DEG C of conditions after continuing percolation, filtration after this thick paste is added etoh solvent, so repeatedly several times after last concentrated namely what obtain be the first thick paste, finally detect its alkaloid.Concentrated is generally take the mode of filtrate being carried out evaporating between 60-70 DEG C to concentrate, and the temperature of evaporation and concentration is unsuitable too high, can destroy effective ingredient wherein thus cause certain loss.
Noticeablely to be, after filtration step, remaining residual substances needs through following treatment step: adjust pH value to acid after first thin up, when dilution is in order to follow-up interpolation PH regulator, its easily and residual substances mix, form homogeneous liquid substance, pH value is preferably adjusted between 2-4, and now chlorophyll can be destroyed completely, chlorophyll molecule is easy to the magnesium lost in porphyrin ring becomes de-magnesium pheophytin, and therefore pH value preferably controls within the scope of this.PH regulator can use dilution heat of sulfuric acid, dilute hydrochloric acid solution etc., and during concrete operations, adding rate is not easily too fast, because neutralization reaction itself belongs to exothermic reaction, therefore preferably controls certain speed.
In the present invention, in order to promote chlorophyll stripping, preferably by thin up and the medicinal liquid obtained after regulating pH value heat, can accelerate the stripping of the chloroplast in chlorophyll like this, the temperature of heating should not be too high, control between 40-70 DEG C, can select 45 DEG C, 50 DEG C or 65 DEG C, the time of heating is also unsuitable long, controls between 5-10min, 6min can be selected, 7min or 8min.As long as that is realize suitable heating, do not heat too quickly or heat time heating time is long, the effective ingredient in such residual substances also can be destroyed.The length of the temperature of heating and the time of heating is all that inventor is gone out by a large amount of optimum experimental, preferably controls, in the scope of afore mentioned rules, to be conducive to the quality of raising second thick paste like this.The mode of heating can adopt the mode such as water-bath, electrical heating, as long as can realize the object heated.
After residual substances being regulated PH to acidity, generally lamination can be there is, upper strata chlorophyll is isolated after layering, collect the clear liquor that target product is contained in lower floor, and clear liquor is adjusted to alkalescence, active substance belladonna alkaloid wherein can be made to dissociate out again, then continue to be concentrated into the second thick paste, same pH value preferably controls between 8-10, within the scope of this, hyoscyami alkaloid more easily dissociates out, PH regulator can use sodium hydroxide solution, potassium hydroxide solution etc., during concrete operations, concentration of lye is unsuitable too high, adding rate is not easily too fast, because neutralization reaction itself belongs to exothermic reaction, therefore certain speed will be controlled well.
The embodiment of the present invention additionally provides a kind of belladonna extract adopting above-mentioned preparation method to obtain, its content of atropine sulfate of belladonna extract adopting preparation method of the present invention to obtain is 9.8-10mg/ml, the content of atropine sulfate of the Semen daturae fluid extract in general standards of pharmacopoeia is at more than 6.6mg/ml, and the content of atropine sulfate of the Semen daturae fluid extract adopting percolation method general in prior art to prepare is between 7-8mg/ml, normal method of producing product is remerged after this effective ingredient by extracting in garbage of the visible employing embodiment of the present invention, the product quality obtained also promotes to some extent, purity also can be ensured accordingly, medical value is high.The preparation method of this Semen daturae fluid extract is suitable for large application scope, not only product good drug efficacy, can directly make medicine and sell, and cost is low, profit is high, has wide economic benefit.
Compared with prior art, beneficial effect of the present invention is:
(1) preparation method of belladonna extract that provides of the embodiment of the present invention, make full use of " chlorophyll " garbage obtained in normal productive process, improve the additional value of garbage, also the impact abandoning garbage and need to consider to abandon the factors such as region and environmental requirement is removed from, not only economy but also environmental protection;
(2) preparation method of the belladonna extract of the embodiment of the present invention is simple to operate, without the need to using toxic chemical substance in operating process, omnidistance green non-pollution, and obtain that belladonna extract purity is high, good drug efficacy, its sulphuric acid Ah taking off product content is between 9.8-10mg/ml, can directly make medicine to sell, there is wide economic benefit;
(3) preparation method of the belladonna extract of the embodiment of the present invention by the creationary invention of special properties in conjunction with this medicine of Semen daturae a kind of preparation method of belladonna extract, especially the number of times of percolation and percolation speed are preferably controlled repeatedly in percolation operation well, fully to extract the effective ingredient in raw material.
Summary of the invention
The first object of the present invention is the preparation method providing a kind of belladonna extract, and described preparation method has that environmental protection, method are simple, to be easy to operation, raw material availability high and the advantage such as to reduce costs.
The second object of the present invention is to provide a kind of described belladonna extract obtained by above-mentioned preparation method, this belladonna extract has good drug efficacy, purity is high, and indices all meets the corresponding requirements of state-promulgated pharmacopoeia, even far above the first-class advantage of its pharmacopeia defined quality index.
In order to realize above-mentioned purpose of the present invention, spy by the following technical solutions:
Embodiments provide a kind of preparation method of belladonna extract, comprise the steps:
(A) by Herba Belladonnae coarse powder after soak with ethanol, percolation, filtration and evaporation and concentration operating procedure, obtain the first thick paste;
(B) residual substances thin up remaining after above-mentioned filtration is isolated upper strata chlorophyll after regulating pH value to acidity, collect lower floor's clear liquor and also adjust pH value to obtain the second thick paste to alkalescence is concentrated;
(C) the first thick paste and the second thick paste are merged, obtain belladonna extract.
Accompanying drawing explanation
In order to be illustrated more clearly in the embodiment of the present invention or technical scheme of the prior art, be briefly described to the accompanying drawing used required in embodiment or description of the prior art below.
Fig. 1 is the liquid chromatograph collection of illustrative plates of the belladonna extract that the embodiment of the present invention one is prepared;
Fig. 2 is the liquid chromatograph collection of illustrative plates of the belladonna extract that comparative example one is prepared;
Fig. 3 is atropine sulfate TLC collection of illustrative plates.
Detailed description of the invention
Below in conjunction with embodiment, embodiment of the present invention are described in detail, but it will be understood to those of skill in the art that the following example only for illustration of the present invention, and should not be considered as limiting the scope of the invention.Unreceipted actual conditions person in embodiment, the condition of conveniently conditioned disjunction manufacturer suggestion is carried out.Agents useful for same or the unreceipted production firm person of instrument, be and can buy by commercially available the conventional products obtained.
Embodiment 1
The preparation method of belladonna extract is as follows:
1) take Herba Belladonnae coarse powder 1kg, adopt mass percent concentration be the ethanol of 85% as solvent, first use soak with ethanol Herba Belladonnae coarse powder 48h, constantly stir to improve uniformity; Then with the speed of 1-3ml/min slowly percolation, treat that hyoscyami alkaloid is filtered out gradually completely, obtain just filtering liquid collecting, and by liquid of just filtering at 60 DEG C of decompression recycling ethanols, after being cooled to room temperature, filtering and also the filtrate obtained evaporation is obtained the first thick paste;
2) after filter operation, the first thin up of remaining residual substances i.e. " chlorophyll " garbage obtains medicinal liquid, then while add dilution heat of sulfuric acid, constantly stir and detect its pH value on one side, when its pH value is that acidity can stop neutralization operation, standing a period of time collects lower floor's clarification medicinal liquid after its layering, adds sodium hydroxide solution, constantly stirs on one side and makes lower floor clarify the pH value of medicinal liquid in alkalescence, stop adding alkali liquor, be then concentrated into second thick paste of relative density at 1.20-1.30;
3) the first thick paste and the second thick paste are merged, adding mass percent concentration is 85% appropriate amount of ethanol, and dilute with water, leave standstill and wait to clarify, namely obtain product after filtration, detecting its content of atropine sulfate is 9.8mg/ml, the visible accompanying drawing 1 of HPLC collection of illustrative plates of its product.
Embodiment 2
The preparation method of belladonna extract is as follows:
1) take Herba Belladonnae coarse powder 1kg, adopt mass percent concentration be the ethanol of 88% as solvent, first use soak with ethanol Herba Belladonnae coarse powder 48h, constantly stir to improve uniformity; Then with the speed of 1-3ml/min slowly percolation, treat that hyoscyami alkaloid is filtered out gradually, obtain just filtering liquid collecting, by liquid of just filtering at 60 DEG C of decompression recycling ethanols, after being cooled to room temperature, filter and after the filtrate obtained is carried out evaporation and concentration at 70 DEG C, add the ethanol of 10 times of quality again, stir with the speed of 1-3ml/min slowly percolation, obtain filtering liquid collecting, liquid will be filtered at 60 DEG C of decompression recycling ethanols, and after being cooled to room temperature, filtering and the filtrate obtained is carried out evaporation and concentration at 70 DEG C obtain the first thick paste;
2) after filter operation, the first thin up of remaining residual substances i.e. " chlorophyll " garbage obtains medicinal liquid, then while add dilution heat of sulfuric acid, constantly stir and detect its pH value between 2-4 on one side, neutralization operation can be stopped, medicinal liquid is heated 10min under 40 DEG C of conditions, and standing 20-30min collects lower floor's clarification medicinal liquid after its layering, while add sodium hydroxide solution, constantly stir on one side and make lower floor clarify the pH value of medicinal liquid between 8-10, stop adding alkali liquor, be then concentrated into second thick paste of relative density at 1.20-1.30;
3) the first thick paste and the second thick paste are merged, adding mass percent concentration is 85% appropriate amount of ethanol, and dilute with water, leave standstill and wait to clarify, namely obtain product after filtration, detecting its content of atropine sulfate is 10mg/ml.
Embodiment 3
The preparation method of belladonna extract is as follows:
1) take Herba Belladonnae coarse powder 1kg, adopt mass percent concentration be the ethanol of 87% as solvent, first use soak with ethanol Herba Belladonnae coarse powder 48h, constantly stir to improve uniformity, then with the speed of 1-3ml/min slowly percolation, treat that hyoscyami alkaloid is filtered out gradually, obtain just filtering liquid collecting, by liquid of just filtering at 60 DEG C of decompression recycling ethanols, after being cooled to room temperature, filter and after the filtrate obtained is carried out evaporation and concentration at 60 DEG C, add the ethanol of 10 times of quality again, stir with the speed of 1-3ml/min slowly percolation, obtain filtering liquid collecting, liquid will be filtered at 60 DEG C of decompression recycling ethanols, after being cooled to room temperature, add again alcohol solvent so repeatedly percolation once after, finally filter and the filtrate obtained is carried out evaporation and concentration at 60 DEG C and obtain the first thick paste,
2) after filter operation, the first thin up of remaining residual substances i.e. " chlorophyll " garbage obtains medicinal liquid, then while add dilution heat of sulfuric acid, constantly stir and detect its pH value between 2-4 on one side, neutralization operation can be stopped, medicinal liquid is heated 5min under 70 DEG C of conditions, and standing 20-30min collects lower floor's clarification medicinal liquid after its layering, while add sodium hydroxide solution, constantly stir on one side and make lower floor clarify the pH value of medicinal liquid between 8-10, stop adding alkali liquor, be then concentrated into second thick paste of relative density at 1.20-1.30;
3) the first thick paste and the second thick paste are merged, adding mass percent concentration is 85% appropriate amount of ethanol, and dilute with water, leave standstill and wait to clarify, namely obtain product after filtration, detecting its content of atropine sulfate is 9.9mg/ml.
Embodiment 4
The preparation method of belladonna extract is as follows:
1) take Herba Belladonnae coarse powder 1kg, adopt mass percent concentration be the ethanol of 86% as solvent, first use soak with ethanol Herba Belladonnae coarse powder 48h, constantly stir to improve uniformity, then with the speed of 1-3ml/min slowly percolation, treat that hyoscyami alkaloid is filtered out gradually, obtain just filtering liquid collecting, by liquid of just filtering at 65 DEG C of decompression recycling ethanols, after being cooled to room temperature, filter and after the filtrate obtained is carried out evaporation and concentration at 60 DEG C, add the ethanol of 10 times of quality again, stir with the speed of 1-3ml/min slowly percolation, obtain filtering liquid collecting, liquid will be filtered at 60 DEG C of decompression recycling ethanols, after being cooled to room temperature, add again alcohol solvent so repeatedly percolation once after, finally filter and the filtrate obtained is carried out evaporation and concentration at 65 DEG C and obtain the first thick paste,
2) after filter operation, the first thin up of remaining residual substances i.e. " chlorophyll " garbage obtains medicinal liquid, then while add dilution heat of sulfuric acid, constantly stir and detect its pH value between 2-4 on one side, neutralization operation can be stopped, medicinal liquid is heated 8min under 60 DEG C of conditions, and standing 20-30min collects lower floor's clarification medicinal liquid after its layering, while add sodium hydroxide solution, constantly stir on one side and make lower floor clarify the pH value of medicinal liquid between 8-10, stop adding alkali liquor, be then concentrated into second thick paste of relative density at 1.20-1.30;
3) the first thick paste and the second thick paste are merged, adding mass percent concentration is 85% appropriate amount of ethanol, and dilute with water, leave standstill and wait to clarify, namely obtain product after filtration, detecting its content of atropine sulfate is 10mg/ml.
Comparative example 1
Get Herba Belladonnae coarse powder 1kg, adopt mass percent concentration be the ethanol of 87% as solvent, first use soak with ethanol Herba Belladonnae coarse powder 48h, constantly stir to improve uniformity; Then with the speed of 1-3ml/min slowly percolation, treat that hyoscyami alkaloid is filtered out gradually, obtain just filtering liquid collecting, by liquid of just filtering at 60 DEG C of decompression recycling ethanols, after being cooled to room temperature, filtering and after the filtrate obtained is carried out evaporation and concentration at 60 DEG C, obtain thick paste, adding mass percent concentration is 85% appropriate amount of ethanol, and dilute with water, leave standstill and wait to clarify, after filtration, namely obtain product, detecting its content of atropine sulfate is 7.4mg/ml, the visible accompanying drawing 1 of HPLC collection of illustrative plates of its product.
Experimental example 1
The quality index of the belladonna extract of the embodiment of the present invention and comparative example and standards of pharmacopoeia, industry standard are contrasted, concrete comparing result is as following table 1:
Table 1 comparing result
| Classification | Content of atropine sulfate | Peak area |
| Standards of pharmacopoeia | ≥6.6mg/ml | ≥6.3％ |
| Embodiment 1 | 9.8mg/ml | 9.3％ |
| Embodiment 2 | 10mg/ml | 9.4％ |
| Embodiment 3 | 9.9mg/ml | 9.4％ |
| Embodiment 4 | 10mg/ml | 9.5％ |
| Comparative example 1 | 7.4mg/ml | 6.95％ |
Detection method in upper table 1 measures according to version " Chinese Pharmacopoeia " the 396th page of " Semen daturae fluid extract " item in 2005, require that the peak area sum of all the other two chromatographic peaks outside sulfuric acid atropine chromatographic peak must not be less than 6.3% of above-mentioned three chromatographic peak total peak areas in its step of 396 pages (2), but the present invention in this index all more than 9%.
Can find out in the embodiment of the present invention that from upper table 1 quality index obtaining belladonna extract is higher, and belladonna extract prepared by the embodiment of the present invention has very high medical value, and from the level that is also in a leading position the same industry, quality is high, applied widely.
Contrast as can be seen from accompanying drawing 1-2, the embodiment of the present invention 1 obtains Semen daturae fluid extract and well coincide with the high-efficient liquid phase chromatogram of the Semen daturae fluid extract adopting the method for existing method and comparative example 1 to obtain, almost consistent, illustrate the every quality index of the products obtained therefrom of the embodiment of the present invention meet 2010 version " Chinese Pharmacopoeia " corresponding requirements.
Accompanying drawing 3 is atropine sulfate TLC collection of illustrative plates, label 1-3 is that the atropine sulfate TLC of the Semen daturae fluid extract that the embodiment of the present invention 1 obtains schemes, label 4-5 is that atropine sulfate reference substance TLC schemes, label 6-8 is that the atropine sulfate TLC of the Semen daturae fluid extract that comparative example 1 obtains schemes, the TLC figure of the product that the visible embodiment of the present invention obtains schemes almost consistent with the TLC normally producing the product obtained, very identical, demonstrate further the every quality index of products obtained therefrom meet 2010 version " Chinese Pharmacopoeia " corresponding requirements.
Utilize the method for Semen daturae fluid extract chlorophyll garbage simple to operation, enterprise's existing equipment and condition can meet its technological requirement completely.In normal Semen daturae fluid extract production process, often extract the chlorophyll garbage that 1000 kilograms of Herba Belladonnaes produce about 23 kilograms, enterprise about produces 2500 kilograms of chlorophyll garbages the whole year, the ointment that therefrom extracting and developing goes out can prepare about 4000 kilograms of Semen daturae fluid extracts, by current 450/ kilogram of sales price, estimate the whole year and can be manufacturing enterprise and realize production value added about 1,800,000 yuan.
Although illustrate and describe the present invention with specific embodiment, however it will be appreciated that can to make when not deviating from the spirit and scope of the present invention many other change and amendment.Therefore, this means to comprise all such changes and modifications belonged in the scope of the invention in the following claims.
Claims (10)
1. a preparation method for belladonna extract, is characterized in that, comprises the steps:
(A) by Herba Belladonnae coarse powder after soak with ethanol, percolation, filtration and evaporation and concentration operating procedure, obtain the first thick paste;
(B) residual substances thin up remaining after above-mentioned filtration is isolated upper strata chlorophyll after regulating pH value to acidity, collect lower floor's clear liquor and also adjust pH value to obtain the second thick paste to alkalescence is concentrated;
(C) the first thick paste and the second thick paste are merged, obtain belladonna extract.
2. the preparation method of belladonna extract according to claim 1, it is characterized in that, in described step (A), the mass percent concentration of ethanol used is 85-88%.
3. the preparation method of belladonna extract according to claim 1, it is characterized in that, in described step (A), percolation 2-3 time repeatedly, the speed of each percolation is 1-3ml/min.
4. the preparation method of belladonna extract according to claim 1, is characterized in that, in described step (A), filters the filtrate obtained and carry out evaporation and concentration between 60-70 DEG C.
5. the preparation method of belladonna extract according to claim 1, it is characterized in that, in described step (B), thin up also regulates pH value to 2-4.
6. the preparation method of belladonna extract according to claim 1, is characterized in that, in described step (B), collects lower floor's clear liquor and also adjusts pH value to 8-10.
7. the preparation method of belladonna extract according to any one of claim 1-6, it is characterized in that, in described step (B), regulate the step of pH value and isolate also to comprise the steps: thin up between the chlorophyllous step in upper strata and the medicinal liquid obtained after regulating pH value heats at thin up.
8. the preparation method of belladonna extract according to claim 7, it is characterized in that, the temperature of heating is 40-70 DEG C, and the time of heating is 5-10min.
9. adopt the belladonna extract that the preparation method of the belladonna extract described in any one of claim 1-8 prepares.
10. belladonna extract according to claim 9, is characterized in that, the content of atropine sulfate of described belladonna extract is 9.8-10mg/ml.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510287958.8A CN104840603A (en) | 2015-05-29 | 2015-05-29 | Belladonna extract and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510287958.8A CN104840603A (en) | 2015-05-29 | 2015-05-29 | Belladonna extract and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104840603A true CN104840603A (en) | 2015-08-19 |
Family
ID=53840849
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510287958.8A Pending CN104840603A (en) | 2015-05-29 | 2015-05-29 | Belladonna extract and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104840603A (en) |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105816521A (en) * | 2016-06-03 | 2016-08-03 | 陈瑞 | Bothriospermum chinense (Turcz.) bunge extract for treating urinary tract infection |
| CN105816581A (en) * | 2016-06-03 | 2016-08-03 | 陈瑞 | Herba verbenae extract for treating heatstroke |
| CN105920214A (en) * | 2016-06-03 | 2016-09-07 | 陈瑞 | Herba violae extract with antihypertensive effect |
| CN107582729A (en) * | 2017-09-29 | 2018-01-16 | 中南民族大学 | A kind of preparation method and applications of belladonna extract |
| CN110859887A (en) * | 2019-11-14 | 2020-03-06 | 安徽恒达药业有限公司 | Percolation extraction method of belladonna extract |
| CN111588775A (en) * | 2020-06-15 | 2020-08-28 | 弘美制药（中国）有限公司 | Belladonna extract and belladonna capsule compound preparation |
| CN111671820A (en) * | 2020-07-22 | 2020-09-18 | 江西省林业科学院 | Method for preparing belladonna fluid extract by bionic compounding |
| CN113274436A (en) * | 2021-04-06 | 2021-08-20 | 福建力传生物有限公司 | Belladonna extract preparation method |
| CN113563326A (en) * | 2021-08-28 | 2021-10-29 | 锦州拾正生物科技有限公司 | Atropine separated and purified from alkaloid extract and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1846752A (en) * | 2006-02-21 | 2006-10-18 | 高陆 | Belladonna extract preparing process |
| CN104173578A (en) * | 2014-09-10 | 2014-12-03 | 湖南科技学院 | Preparation method of belladonna extract |
-
2015
- 2015-05-29 CN CN201510287958.8A patent/CN104840603A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1846752A (en) * | 2006-02-21 | 2006-10-18 | 高陆 | Belladonna extract preparing process |
| CN104173578A (en) * | 2014-09-10 | 2014-12-03 | 湖南科技学院 | Preparation method of belladonna extract |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105816521A (en) * | 2016-06-03 | 2016-08-03 | 陈瑞 | Bothriospermum chinense (Turcz.) bunge extract for treating urinary tract infection |
| CN105816581A (en) * | 2016-06-03 | 2016-08-03 | 陈瑞 | Herba verbenae extract for treating heatstroke |
| CN105920214A (en) * | 2016-06-03 | 2016-09-07 | 陈瑞 | Herba violae extract with antihypertensive effect |
| CN107582729A (en) * | 2017-09-29 | 2018-01-16 | 中南民族大学 | A kind of preparation method and applications of belladonna extract |
| CN110859887A (en) * | 2019-11-14 | 2020-03-06 | 安徽恒达药业有限公司 | Percolation extraction method of belladonna extract |
| CN111588775A (en) * | 2020-06-15 | 2020-08-28 | 弘美制药（中国）有限公司 | Belladonna extract and belladonna capsule compound preparation |
| CN111588775B (en) * | 2020-06-15 | 2022-01-18 | 弘美制药（中国）有限公司 | Belladonna extract and belladonna capsule compound preparation |
| CN111671820A (en) * | 2020-07-22 | 2020-09-18 | 江西省林业科学院 | Method for preparing belladonna fluid extract by bionic compounding |
| CN113274436A (en) * | 2021-04-06 | 2021-08-20 | 福建力传生物有限公司 | Belladonna extract preparation method |
| CN113563326A (en) * | 2021-08-28 | 2021-10-29 | 锦州拾正生物科技有限公司 | Atropine separated and purified from alkaloid extract and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104840603A (en) | Belladonna extract and preparation method thereof | |
| CN105130939B (en) | A kind of method that cyanidenon is extracted from peanut shell | |
| CN104710391A (en) | Method for extracting luteolin and beta-sitosterol from peanut shells | |
| CN1028604C (en) | Prepn. of Chinese herb-fresh bamboo-juice | |
| CN108179065A (en) | A kind of extracting method of jasmine essential oil | |
| CN110882329A (en) | Belladonna liquid extract production process | |
| CN103211926A (en) | Method for extracting total flavonoids in grape seeds in double-aqueous phase system | |
| CN107586597A (en) | A kind of processing method of tea seed | |
| CN104726250B (en) | The brewage process of chestnut flower boy's wine | |
| CN108863524A (en) | A kind of dedicated Selenium-rich nutrient solution of selenium-enriched tea leaf and preparation method thereof | |
| CN113274436A (en) | Belladonna extract preparation method | |
| CN104721121B (en) | A kind of skin-care cosmetics | |
| CN101602996B (en) | Health preserving and health care wine and preparation method thereof | |
| CN101611878B (en) | Method for preparing carotenoids in gac fruit | |
| CN103073561B (en) | Process of extracting artemisinin by biological enzyme-percolation method | |
| CN106753912A (en) | A kind of shinyleaf yellowhorn oil handmade soap and preparation method thereof | |
| CN101177448A (en) | Method for extracting mersosin by ultrasonic field assisted with mixed solvent | |
| CN103255022B (en) | Eucommia ulmoides male flower wine and preparation method thereof | |
| CN105497101A (en) | Method for extracting kinds of combined extract from blueberry stems and leaves | |
| CN105131005A (en) | Preparation method for high content macleaya cordata alkaloid | |
| CN108142550A (en) | A kind of fresh walnut kernel plastics containing Artemisinin and preparation method thereof | |
| KR101687447B1 (en) | Method for isolating and removing chlorophyll from natural material | |
| CN103159597A (en) | Comprehensive exploration and utilization of active ingredients in branches and leaves of weeping willows | |
| CN105963479A (en) | Method for extracting paris polyphylla saponin | |
| CN102058626A (en) | Method for extracting effective components from hippochaete ramosissimum |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| EXSB | Decision made by sipo to initiate substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20150819 |