CN107586597A - A kind of processing method of tea seed - Google Patents
A kind of processing method of tea seed Download PDFInfo
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- CN107586597A CN107586597A CN201710942181.3A CN201710942181A CN107586597A CN 107586597 A CN107586597 A CN 107586597A CN 201710942181 A CN201710942181 A CN 201710942181A CN 107586597 A CN107586597 A CN 107586597A
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Abstract
The invention discloses a kind of processing method of tea seed, after camellia oleifera fruit dries, peel off shell, obtain tea seed, then the seed shell of tea seed is peelled off, obtain camellia seed kernel, camellia seed kernel crushes, with hot-water soak, filtering, obtain the first filtrate and the first filter residue, first filtrate concentrates, dry, obtain Tea Saponin, ethanol solution is added in first filter residue, first carry out ultrasonic wave extraction, carry out Microwave Extraction, filtering, obtain the second filtrate and the second filter residue, second filtrate concentrates, reclaim ethanol, dry, obtain Tea Saponin, cellulase solution is added in second filter residue to be digested, digest liquid precipitate, collect top layer oil, obtain tea-seed oil.This method first extracts Tea Saponin from tea seed, then extracts tea-seed oil.When extracting Tea Saponin, first extracted with hot water, then extracted with ethanol, carried out when ethanol extracts with ultrasonic microwave auxiliary, the recovery rate of Tea Saponin is high.Tea-seed oil is extracted using the method for enzymolysis, tea seed well cuts are few, quality better.
Description
Technical field
The invention belongs to edible oil processing method technical field, in particular it relates to a kind of deep working method of tea-seed oil.
Background technology
Tea seed (Camellia seed), also known as camellia seed, it is the fruit of tea oil tree.Tea oil tree is the main wood in China
This tallow tree, it is described as " east tree ", belongs to Theaceae Camellia evergreen dungarunga, is the distinctive woody vegetable oil money in China
Source.3-6 meters of the height of tree, for the diameter of a cross-section of a tree trunk 1.3 meters above the ground up to 24-30 centimetres, smooth bark is taupe.Tea oil tree list leaf alternate, flower are white for both sexes
Color, October Post flowering until fruit between October next year can be ripe, therefore, tea oil tree has rare flowers and fruits homophyletic phenomenon.Fruit
Actually capsule, it is mostly ellipse, has fine, soft fur.Seed is mostly yellowish-brown, glossy, triangular shape.Tea oil tree is grown in remote mountains, inhales day to the greatest extent
Right nutrient, evergreen longevity, the age of tree was up to more than 200 years.
Tea-seed oil, also known as camellia oil, be derived from the seed of tea oil tree, be free of erucic acid in tea-seed oil, cholesterol, aflatoxins and
Other additives.After tested：Unrighted acid is up to more than 90% in tea-seed oil, and oleic acid reaches 80-83%, and linoleic acid reaches
7-13%, and be rich in protein and vitamin A, B, D, E etc., the abundant leukotrienes especially contained by it be it is needed by human and
It can not synthesize.
Tea Saponin also known as TS, belong to triterpenes saponin, there is toil pungent, be a kind of natural table of good performance
Face activating agent, it can be widely applied to light industry, chemical industry, agricultural chemicals, feed, cultivation, weaving, oil recovery, mining, building materials and highway
The fields such as construction.
In the prior art, tea seed is first extracted oil, and extracts Tea Saponin in the tea bran after oil expression again, this method tea-seed oil
Post-process comparatively laborious, the recovery rate of Tea Saponin is relatively low.
The content of the invention
The invention aims to overcome drawbacks described above, there is provided a kind of processing method of tea seed.
The purpose of the present invention implements by the following technical programs：
A kind of processing method of tea seed, after camellia oleifera fruit dries, shell is peelled off, tea seed is obtained, then peels off tea seed
Seed shell, obtain camellia seed kernel, camellia seed kernel crushes, and with hot-water soak, filtering, obtains the first filtrate and the first filter residue, the first filter
Liquid concentrates, and dries, obtains Tea Saponin, ethanol solution is added in the first filter residue, first carries out ultrasonic wave extraction, carries out Microwave Extraction, mistake
Filter, the second filtrate and the second filter residue are obtained, the concentration of the second filtrate, ethanol is reclaimed, dries, obtained Tea Saponin, added in the second filter residue fine
Tie up plain enzyme solutions to be digested, digest liquid precipitate, collect top layer oil, obtain tea-seed oil.
Further, methods described specifically comprises the following steps：
(1) pretreatment of camellia oleifera fruit
After camellia oleifera fruit collection, dry, peel off shell, obtain tea seed, then peel off the seed shell of tea seed, obtain tea seed
Benevolence；
(2) hot-water soak extraction Tea Saponin
The camellia seed kernel that step (1) obtains is crushed, and obtained camellia seed kernel particle is carried out secondary with hot-water soak method
Extraction, filtering, filtrate merge, and obtain the first filtrate and the first filter residue, and the first filtrate is concentrated under reduced pressure, and concentrate is dried, and is obtained
Tea Saponin；
(3) organic solvent extraction Tea Saponin
Using ethanol solution as solvent, the first filter residue obtained using ultrasonic-microwave method to step (2) carries out secondary carry
Take, filter, filtrate merges, and obtains the second filtrate and the second filter residue, and the second filtrate is concentrated under reduced pressure, and reclaims ethanol, concentrate
Dry, obtain Tea Saponin；
(4) enzymolysis and extraction tea-seed oil
Cellulase solution is added in the second filter residue that step (3) obtains, carries out enzyme digestion reaction, collects enzymolysis liquid, enzymolysis
Liquid is stood, and is collected top layer oil, is obtained tea-seed oil.
Further, in the step (1), it is 8-10 days to dry the time.
Further, in the step (2), the temperature of the hot water is 90-95 DEG C, and amount of hot water is during immersion every time
3-4 times of camellia seed kernel particle weight.
Further, in the step (2), each soak time is 15-20min, and hot water first stirs 3min when adding.
Further, in the step (3), the volume fraction of the ethanol solution is 60-70%.
Further, in the step (3), the dosage of ethanol solution is the 2-3 of the first filter residue weight during extraction every time
Times.
Further, in the step (3), when extracting every time, ultrasonic wave extraction 10-15min is first carried out, then microwave
Extract 50-100s.
Further, in the step (4), the concentration of the cellulase solution is 6-10mg/L, the pH of enzymolysis liquid
It is 4-5 times of the second filter residue weight to be worth for 4.7-5.2, addition；
Enzyme digestion reaction carries out 3-4h at 45-50 DEG C.
Further, in the step (4), time of repose 12-16h.
The processing method of the tea seed of the present invention, the Tea Saponin in tea seed is first extracted, then extract tea-seed oil.Tea Saponin carries
When taking, first with hot water extraction, then extracted with ethanol, ethanol uses ultrasonic-microwave combined extraction method when extracting, not only can be with
Recovery rate is improved, also beneficial to the extraction of follow-up tea-seed oil.Extract the residue Enzymatic Extraction tea-seed oil after Tea Saponin.
The invention has the advantages that：
The processing method of tea seed provided by the invention, Tea Saponin is first extracted from tea seed, then extract tea-seed oil, it is different
In the common method first extracted oil and extract Tea Saponin again.When extracting Tea Saponin, first extracted with hot water, then extracted with ethanol, ethanol
Carried out during extraction with ultrasonic-microwave auxiliary, the recovery rate of Tea Saponin is high.Tea-seed oil, tea-seed oil are extracted using the method for enzymolysis
Middle impurity is few, quality better.
Embodiment
The preferred embodiments of the present invention are illustrated below, it will be appreciated that preferred embodiment described herein is only used
In the description and interpretation present invention, it is not intended to limit the present invention.
Embodiment 1
A kind of processing method of tea seed, comprises the following steps：
(1) pretreatment of camellia oleifera fruit
After camellia oleifera fruit collection, dry 10 days, peel off shell, obtain tea seed, then peel off the seed shell of tea seed, obtain oil
Tea seed kernel.
(2) hot-water soak extraction Tea Saponin
Camellia seed kernel is crushed, and obtained camellia seed kernel particle carries out secondary soak extraction with 90-95 DEG C of hot water.First
Amount of hot water is 4 times of camellia seed kernel particle weight during secondary extraction, soak time 20min.Amount of hot water is during second of extraction
3 times of camellia seed kernel particle weight, soak time 15min.After adding hot water every time, 3min is first stirred.Filtered after immersion, filtrate
Merge, obtain the first filtrate and the first filter residue, the first filtrate is concentrated under reduced pressure, and concentrate is dried, and obtains Tea Saponin.
(3) organic solvent extraction Tea Saponin
Using 60vt% ethanol solutions as solvent, second extraction is carried out to the first filter residue.When extracting for the first time, ethanol solution
Addition is 3 times of the first filter residue weight, first carries out ultrasonic wave extraction 10min, then Microwave Extraction 100s.Second of extraction
When, ethanol solution addition is 2 times of the first filter residue weight, first carries out ultrasonic wave extraction 15min, then Microwave Extraction 50s.Carry
Filtered after taking, filtrate merges, and obtains the second filtrate and the second filter residue, and the second filtrate is concentrated under reduced pressure, and reclaims ethanol, concentrate
Dry, obtain Tea Saponin.
(4) enzymolysis and extraction tea-seed oil
Add the cellulase solution of 5 times of its weight into the second filter residue, cellulase solution concentration is 10mg/L, pH value
Between 4.7-5.2, enzyme digestion reaction 3h is carried out at 50 DEG C, collects enzymolysis liquid, enzymolysis liquid stands 16h, collects top layer oil, obtains
Tea-seed oil.
Embodiment 2
A kind of processing method of tea seed, comprises the following steps：
(1) pretreatment of camellia oleifera fruit
After camellia oleifera fruit collection, dry 8 days, peel off shell, obtain tea seed, then peel off the seed shell of tea seed, obtain oil
Tea seed kernel.
(2) hot-water soak extraction Tea Saponin
Camellia seed kernel is crushed, and obtained camellia seed kernel particle carries out secondary soak extraction with 90-95 DEG C of hot water.First
Amount of hot water is 3 times of camellia seed kernel particle weight during secondary extraction, soak time 15min.Amount of hot water is during second of extraction
4 times of camellia seed kernel particle weight, soak time 20min.After adding hot water every time, 3min is first stirred.Filtered after immersion, filtrate
Merge, obtain the first filtrate and the first filter residue, the first filtrate is concentrated under reduced pressure, and concentrate is dried, and obtains Tea Saponin.
(3) organic solvent extraction Tea Saponin
Using 70vt% ethanol solutions as solvent, second extraction is carried out to the first filter residue.When extracting for the first time, ethanol solution
Addition is 2 times of the first filter residue weight, first carries out ultrasonic wave extraction 15min, then Microwave Extraction 50s.During second of extraction,
Ethanol solution addition is 3 times of the first filter residue weight, first carries out ultrasonic wave extraction 10min, then Microwave Extraction 100s.Extraction
After filter, filtrate merges, and obtains the second filtrate and the second filter residue, and the second filtrate is concentrated under reduced pressure, and reclaims ethanol, concentrate does
It is dry, obtain Tea Saponin.
(4) enzymolysis and extraction tea-seed oil
Add the cellulase solution of 4 times of its weight into the second filter residue, cellulase solution concentration is 6mg/L, pH value
Between 4.7-5.2, enzyme digestion reaction 4h is carried out at 45 DEG C, collects enzymolysis liquid, enzymolysis liquid stands 12h, collects top layer oil, obtains
Tea-seed oil.
The preferred embodiments of the present invention are the foregoing is only, are not intended to limit the invention, although with reference to foregoing reality
Apply example the present invention is described in detail, for those skilled in the art, it still can be to foregoing each implementation
Technical scheme described in example is modified, or carries out equivalent substitution to which part technical characteristic.All essences in the present invention
God any modification, equivalent substitution and improvements made etc., should be included in the scope of the protection with principle.
Claims (10)
1. a kind of processing method of tea seed, it is characterised in that after camellia oleifera fruit dries, peel off shell, obtain tea seed, then shell
The seed shell of tea seed is removed, obtains camellia seed kernel, camellia seed kernel crushes, and with hot-water soak, filtering, obtains the first filtrate and the first filter
Slag, the first filtrate concentration, dries, obtains Tea Saponin, ethanol solution is added in the first filter residue, first carries out ultrasonic wave extraction, carries out micro-
Ripple extracts, filtering, obtains the second filtrate and the second filter residue, the concentration of the second filtrate, reclaims ethanol, dry, obtain Tea Saponin, the second filter residue
Middle addition cellulase solution is digested, and digests liquid precipitate, is collected top layer oil, is obtained tea-seed oil.
2. the processing method of tea seed according to claim 1, it is characterised in that methods described specifically includes following step
Suddenly：
(1) pretreatment of camellia oleifera fruit
After camellia oleifera fruit collection, dry, peel off shell, obtain tea seed, then peel off the seed shell of tea seed, obtain camellia seed kernel；
(2) hot-water soak extraction Tea Saponin
The camellia seed kernel that step (1) obtains is crushed, and obtained camellia seed kernel particle carries out second extraction with hot-water soak method,
Filtering, filtrate merge, and obtain the first filtrate and the first filter residue, and the first filtrate is concentrated under reduced pressure, and concentrate is dried, and obtains tea soap
Element；
(3) organic solvent extraction Tea Saponin
Using ethanol solution as solvent, the first filter residue obtained using ultrasonic-microwave method to step (2) carries out second extraction,
Filtering, filtrate merge, and obtain the second filtrate and the second filter residue, and the second filtrate is concentrated under reduced pressure, and reclaim ethanol, and concentrate is done
It is dry, obtain Tea Saponin；
(4) enzymolysis and extraction tea-seed oil
Cellulase solution is added in the second filter residue that step (3) obtains, carries out enzyme digestion reaction, collects enzymolysis liquid, enzymolysis liquid is quiet
Put, collect top layer oil, obtain tea-seed oil.
3. the processing method of tea seed according to claim 2, it is characterised in that in the step (1), the drying time is
8-10 days.
4. the processing method of tea seed according to claim 2, it is characterised in that in the step (2), the hot water
Temperature is 90-95 DEG C, and amount of hot water is 3-4 times of camellia seed kernel particle weight during immersion every time.
5. the processing method of tea seed according to claim 2, it is characterised in that in the step (2), when soaking every time
Between be 15-20min, hot water first stirs 3min when adding.
6. the processing method of tea seed according to claim 2, it is characterised in that in the step (3), the ethanol is molten
The volume fraction of liquid is 60-70%.
7. the processing method of tea seed according to claim 2, it is characterised in that in the step (3), when extracting every time
The dosage of ethanol solution is 2-3 times of the first filter residue weight.
8. the processing method of tea seed according to claim 2, it is characterised in that in the step (3), extraction every time
When, ultrasonic wave extraction 10-15min is first carried out, then Microwave Extraction 50-100s.
9. the processing method of tea seed according to claim 2, it is characterised in that in the step (4), the cellulose
The concentration of enzyme solutions is 6-10mg/L, and the pH value of enzymolysis liquid is 4.7-5.2, and addition is 4-5 times of the second filter residue weight；
Enzyme digestion reaction carries out 3-4h at 45-50 DEG C.
10. the processing method of tea seed according to claim 2, it is characterised in that in the step (4), time of repose
For 12-16h.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108550832A (en) * | 2018-05-15 | 2018-09-18 | 肇庆益晟商贸有限公司 | A kind of anode material of lithium battery and preparation method thereof of rare earth doped element |
| CN108690728A (en) * | 2018-07-26 | 2018-10-23 | 贵州夜郎古国生物科技有限公司 | A kind of preparation method of tea seed essential oil |
| CN108865413A (en) * | 2018-08-13 | 2018-11-23 | 安徽兆龙生物科技有限公司 | A kind of tea-seed oil low-temperature cold wind extraction process |
| CN109498515A (en) * | 2018-12-23 | 2019-03-22 | 广州素颜皮肤管理科技有限公司 | It is a kind of have effects that repair extracts of pinus koraiensis and its application |
| CN111690459A (en) * | 2020-05-18 | 2020-09-22 | 六安市裕安区伊甸园油茶种植专业合作社 | Method for extracting tea seed oil |
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| CN101569329A (en) * | 2009-04-28 | 2009-11-04 | 韶关学院 | Method for extracting tea-seed oil by aqueous enzymatic method |
| CN101812111A (en) * | 2010-04-19 | 2010-08-25 | 韶关学院 | Comprehensive deep processing method for oil tea fruits |
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| CN101569329A (en) * | 2009-04-28 | 2009-11-04 | 韶关学院 | Method for extracting tea-seed oil by aqueous enzymatic method |
| CN101812111A (en) * | 2010-04-19 | 2010-08-25 | 韶关学院 | Comprehensive deep processing method for oil tea fruits |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108550832A (en) * | 2018-05-15 | 2018-09-18 | 肇庆益晟商贸有限公司 | A kind of anode material of lithium battery and preparation method thereof of rare earth doped element |
| CN108690728A (en) * | 2018-07-26 | 2018-10-23 | 贵州夜郎古国生物科技有限公司 | A kind of preparation method of tea seed essential oil |
| CN108865413A (en) * | 2018-08-13 | 2018-11-23 | 安徽兆龙生物科技有限公司 | A kind of tea-seed oil low-temperature cold wind extraction process |
| CN109498515A (en) * | 2018-12-23 | 2019-03-22 | 广州素颜皮肤管理科技有限公司 | It is a kind of have effects that repair extracts of pinus koraiensis and its application |
| CN111690459A (en) * | 2020-05-18 | 2020-09-22 | 六安市裕安区伊甸园油茶种植专业合作社 | Method for extracting tea seed oil |
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