CN101569329A - Method for extracting tea-seed oil by aqueous enzymatic method - Google Patents
Method for extracting tea-seed oil by aqueous enzymatic method Download PDFInfo
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- CN101569329A CN101569329A CNA2009100390393A CN200910039039A CN101569329A CN 101569329 A CN101569329 A CN 101569329A CN A2009100390393 A CNA2009100390393 A CN A2009100390393A CN 200910039039 A CN200910039039 A CN 200910039039A CN 101569329 A CN101569329 A CN 101569329A
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Abstract
The invention relates to a method for extracting tea-seed oil by an aqueous enzymatic method, which comprises the following processing steps: 1, removing tea saponin in the tea-seed oil; 2, pretreating the residue of the tea-seed oil; 3, performing zymohydrolysis; and 4, recovering the tea-seed oil. The tea saponin is firstly removed from the tea-seed oil so as to be favorable for the extraction yield of free oil in a later process; and the method has the advantages of simple processing equipment, safe operation, less pollution, high free oil yield, no residue of organic solvent, and capacity of obtaining high-quality finished product tea oil, and is suitable for large scale production.
Description
[technical field]
The invention belongs to biotechnology in the Application in Food Industry technical field, relate to a kind of method of from the tea seed, removing Tea Saponin earlier, helping the aqueous enzymatic extraction tea-seed oil of postorder free oil recovery rate.
[background technology]
Tea seed (Camellia oleifera seed) also claims the camellia seed, is one of the world's four big woody oleiferous plants, mainly is distributed in Chinese Jiangxi, Hunan, Anhui, Guangxi Si Sheng, and is maximum with the Hunan.In addition, have only ground such as Southeast Asia, Japan that the distribution of minute quantity is arranged.Tea-seed oil with olive oil and be called the world's two big woody edible oils, has the title of " treasure in the oil " from ancient times.Check confirms that the quality of tea oil is better than olive oil through Chinese Center for Disease Control, and high-grade refining tea oil is compared with olive oil, and the nutrition and health care quality is higher.Olive oil contains unrighted acid 75%～90%, and the unsaturated fatty acid content of tea oil is the hat of various edible oils up to 85%～97%.According to Shanghai two medical university's investigation results, tea oil can effectively improve the rising of cardiovascular and cerebrovascular disease, reduction cholesterol and fasting blood-glucose, inhibition triglyceride, helps " three height " patient to improve health status.The scholarly forecast of Ministry of Agriculture's oil plant and quality of item supervision inspection center is said, green, health has become a kind of irresistible fashion, be in the tea seed of nature grows wild state, be subjected to the pollution of chemical fertilizer, agricultural chemicals hardly, do not contain carcinogen aflatoxins and harmful erucic acid and gossypol etc., be real natural, green, healthy food, must be subjected to the welcome in market.
In the tea seed producing region, tea oil is produced the workshop-based squeezing process of most employing, not only cost height, oil yield are low, and owing to contain more recuding sugars in the tea seed, in expressing process, grease color is deepened because of being subjected to high temperature that brown stain easily takes place, make the oil quality difference and price is not high, purposes is restricted, and its press cake also is difficult to utilize.Also have factory to adopt lixiviation process, but the organic solvent that adopts is volatile, is difficult to satisfy requirement of safe production for midget plant, the cost height, big for environment pollution.And easy residual solvent in the product oil, even exceed standard, the harm consumer is healthy.Therefore, press for a kind of new technology and improve present production status.
[summary of the invention]
In order to overcome the above-mentioned shortcoming of prior art, the invention provides a kind ofly have that technology is simple, equipment investment is few, handling safety, pollution less, residual, the free oil yield height of organic solvent-free, can obtain the method for the aqueous enzymatic extraction tea-seed oil of high-quality finished product tea oil and suitable large-scale production.
The technical solution adopted for the present invention to solve the technical problems is: a kind of method of aqueous enzymatic extraction tea-seed oil, and its processing step is:
The first step is removed the Tea Saponin in the tea seed: the Tea Saponin with in organic solvent extraction or the water extract method removal tea seed, separate obtaining the tea seed residue;
Second step was carried out preliminary treatment to the tea seed residue: reduction vaporization reclaims the organic solvent in the tea seed residue, the tea seed residue that obtains is directly used in the enzymolysis and extraction grease, or tea seed residue is again through after the high steam processing, be used for the enzymolysis and extraction grease, or tea seed residue proposes processing through alkali again, is used for the enzymolysis and extraction grease;
The 3rd step was carried out enzymolysis: the tea seed residue of above-mentioned processing is mixed with the ratio of mass volume ratio 1:3～10 with water, the complex cellulase that adds tea seed residue quality 1.0%～5%, under the condition of 50 ℃ of pH5.0 and temperature, stirred enzymolysis 2～5 hours, or first addition is the protease of tea seed residue quality 1.0%, under certain pH and temperature, stirred enzymolysis 1～2 hour, regulate pH to 5.0 then, addition is the complex cellulase of tea seed residue quality 1.0%～5%, 50 ℃ of temperature stirred enzymolysis 2～5 hours;
The 4th step was reclaimed tea-seed oil: enzymolysis liquid directly carries out centrifugation, separates obtaining free oil, emulsion, hydrolyzate and slag, or static placement enzymolysis liquid, reclaims the top layer free oil.
The organic solvent of the Tea Saponin in the described removal tea seed adopts methyl alcohol, ethanol or acetone.
It is heat treated tea seed residue under pressurized conditions that described high steam is handled tea seed residue, and temperature range is 110 ℃～121 ℃, and heat time heating time, scope was 10～20 minutes.
The process conditions that described alkali is carried are to regulate tea seed residue slurries pH to 8～9,50 ℃～70 ℃ of temperature, stirring in water bath 90～100min.
Described tea seed residue mixes by mass volume ratio with water, and its proportion is 1: 4～1: 7 times.
Described protease includes neutral proteinase, alkali protease and acid protease.
The invention has the beneficial effects as follows: the Tea Saponin that contains in the tea seed is a kind of surface-active material that has, and its existence can influence aqueous enzymatic method extracts free oil from the tea seed yield, removes Tea Saponin from the tea seed earlier, helps the recovery rate of postorder free oil; The present invention has that process equipment is simple, handling safety, pollution less, free oil yield height, organic solvent-free be residual, can obtain the advantage of high-quality finished product tea oil, is fit to large-scale production.
[description of drawings]
Fig. 1 is a technological process block diagram of the present invention.
[specific embodiment]
The present invention is further described below in conjunction with drawings and Examples:
Referring to Fig. 1, a kind of method of aqueous enzymatic extraction tea-seed oil, its processing step is: the first step is removed the Tea Saponin in the tea seed: with the Tea Saponin in organic solvent extraction or the water extract method removal tea seed, separate obtaining the tea seed residue, the organic solvent of removing the Tea Saponin in the tea seed adopts methyl alcohol, ethanol or acetone.Second step was carried out preliminary treatment to the tea seed residue: reduction vaporization reclaims the organic solvent in the tea seed residue, the tea seed residue that obtains is directly used in the enzymolysis and extraction grease, or after handling through high steam again, be used for the enzymolysis and extraction grease, wherein high steam processing tea seed residue is a heat treated tea seed residue under pressurized conditions, temperature range is preferably 110 ℃～121 ℃, heat time heating time, scope was preferably 10～20 minutes, or after proposing processing through alkali again, be used for the enzymolysis and extraction grease, wherein to carry and handle tea seed residue be to regulate about tea seed residue slurries pH to 8.8 to alkali, and about 60 ℃ of temperature are about stirring in water bath 95min.The 3rd step was carried out enzymolysis: the tea seed residue of above-mentioned processing is mixed with the ratio of mass volume ratio 1: 3～10 with water, its proportion is preferably 1: 4～and 1: 7 times, addition is the complex cellulase of tea seed residue quality 1.0%～5%, under the condition of 50 ℃ of pH5.0 and temperature, stirred enzymolysis 2～5 hours, perhaps first addition is the protease of tea seed residue quality 1.0%, wherein protease includes neutral proteinase, alkali protease and acid protease, as adopting neutral proteinase, under the condition that pH7.0 and temperature are 50 ℃, stirred enzymolysis 1～2 hour, regulate pH to 5.0 then, addition is the complex cellulase of tea seed residue quality 1.0%～5%, 50 ℃ of temperature stirred enzymolysis 2～5 hours;
The 4th step was reclaimed tea-seed oil: enzymolysis liquid directly carries out centrifugation, separates obtaining free oil, emulsion, hydrolyzate and slag, or static placement enzymolysis liquid, reclaims the top layer free oil.
Embodiment: take by weighing big fruit Semen Camelliae residue 3 grams (weight in wet base) of removing Tea Saponin, 121 ℃ of high steams were handled 15 minutes, sodium hydrogen phosphate-citrate buffer solution the 10ml that adds pH5.0 then, addition is the complex cellulase of tea seed residue quality 3.0%, 50 ℃ of enzymolysis 2 hours, after enzymolysis finishes, carry out centrifugation, collect free oil, drying is weighed, calculate the free oil yield, can reach 85%.
Claims (6)
1, a kind of method of aqueous enzymatic extraction tea-seed oil is characterised in that its processing step is:
The first step is removed the Tea Saponin in the tea seed: the Tea Saponin with in organic solvent extraction or the water extract method removal tea seed, separate obtaining the tea seed residue;
Second step was carried out preliminary treatment to the tea seed residue: reduction vaporization reclaims the organic solvent in the tea seed residue, the tea seed residue that obtains is directly used in the enzymolysis and extraction grease, or tea seed residue is again through after the high steam processing, be used for the enzymolysis and extraction grease, or tea seed residue proposes processing through alkali again, is used for the enzymolysis and extraction grease;
The 3rd step was carried out enzymolysis: the tea seed residue of above-mentioned processing is mixed with the ratio of mass volume ratio 1: 3～10 with water, the complex cellulase that adds tea seed residue quality 1.0%～5%, under the condition of 50 ℃ of pH5.0 and temperature, stirred enzymolysis 2～5 hours, or first addition is the protease of tea seed residue quality 1.0%, under certain pH and temperature, stirred enzymolysis 1～2 hour, regulate pH to 5.0 then, addition is the complex cellulase of tea seed residue quality 1.0%～5%, 50 ℃ of temperature stirred enzymolysis 2～5 hours;
The 4th step was reclaimed tea-seed oil: enzymolysis liquid directly carries out centrifugation, separates obtaining free oil, emulsion, hydrolyzate and slag, or static placement enzymolysis liquid, reclaims the top layer free oil.
2, the method for aqueous enzymatic extraction tea-seed oil as claimed in claim 1 is characterized in that: the organic solvent of the Tea Saponin in the described removal tea seed adopts methyl alcohol, ethanol or acetone.
3, the method for aqueous enzymatic extraction tea-seed oil as claimed in claim 1 is characterized in that: it is heat treated tea seed residue under pressurized conditions that described high steam is handled tea seed residue, and temperature range is 110 ℃～121 ℃, and heat time heating time, scope was 10～20 minutes.
4, the method for aqueous enzymatic extraction tea-seed oil as claimed in claim 1 is characterized in that: the process conditions that described alkali is carried are to regulate tea seed residue slurries pH to 8～9,50 ℃～70 ℃ of temperature, stirring in water bath 90～100min.
5, the method for aqueous enzymatic extraction tea-seed oil as claimed in claim 1 is characterized in that: described tea seed residue mixes by mass volume ratio with water, and its proportion is 1: 4～1: 7 times.
6, the method for aqueous enzymatic extraction tea-seed oil as claimed in claim 1 is characterized in that: described protease includes neutral proteinase, alkali protease and acid protease.
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| CN102816632A (en) * | 2012-09-10 | 2012-12-12 | 韶关学院 | Method for digesting grease through microwave pretreatment and aqueous enzymatic method |
| CN102952624A (en) * | 2012-11-06 | 2013-03-06 | 安徽新世界绿洲茶油有限公司 | Preparation process for strong-flavor tea-seed oil and prepared strong-flavor tea-seed oil |
| CN103205310A (en) * | 2013-05-08 | 2013-07-17 | 长沙理工大学 | Method for sequentially extracting tea saponin and tea seed oil from oil-tea camellia seeds |
| CN103275804A (en) * | 2013-05-10 | 2013-09-04 | 福建省岳秀农林有限公司 | Health protection tea-seed oil and manufacturing technology thereof |
| CN103773591A (en) * | 2014-02-17 | 2014-05-07 | 广西巴马印象生活体验产业有限公司 | Method for extracting tea oil |
| CN104804899A (en) * | 2015-03-31 | 2015-07-29 | 湖南农业大学 | Method for preparing scrubbing solution basic liquid by recycling wastewater produced in oil-refining process with aqueous enzymatic method |
| CN105238551A (en) * | 2015-10-09 | 2016-01-13 | 福建天斯顿生物科技有限公司 | Method for supercritical CO2 extraction and purification of camellia seed oil |
| CN106318599A (en) * | 2016-08-31 | 2017-01-11 | 浙江工业大学 | Method for increasing yield of camellia seed oil extracted with aqueous enzymatic method |
| CN106565812A (en) * | 2016-09-26 | 2017-04-19 | 中国林业科学研究院亚热带林业研究所 | Efficient recovery process for tea saponin continuous extraction solvent |
| CN107183225A (en) * | 2017-05-26 | 2017-09-22 | 钦州市桂耘贸易有限公司 | A kind of anti-oxidant tea oil extract and preparation method thereof |
| CN107334678A (en) * | 2017-07-18 | 2017-11-10 | 安徽省华银茶油有限公司 | A kind of method that antibacterial wet tissue is prepared using camellia seed dregs of rice extract |
| CN107474937A (en) * | 2017-10-13 | 2017-12-15 | 三江县稻香山茶油生态发展有限公司 | A kind of method of Enzymatic Extraction tea-seed oil |
| CN107586597A (en) * | 2017-10-11 | 2018-01-16 | 三江县稻香山茶油生态发展有限公司 | A kind of processing method of tea seed |
| CN107653055A (en) * | 2017-09-29 | 2018-02-02 | 浙江工业大学 | A kind of method of ultrasonic wave added extracting tea seed oil by using enzymatic hydrolysis |
| CN108192718A (en) * | 2018-01-05 | 2018-06-22 | 贵州省香瑞莱香料有限公司 | A kind of method that aqueous enzymatic method extracts seed oil of Moringa oleigera and protein hydrolysate simultaneously |
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| CN114794468A (en) * | 2022-04-22 | 2022-07-29 | 贵州山茶产业综合开发有限公司 | In-situ camellia oil emulsion and preparation method and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102816632A (en) * | 2012-09-10 | 2012-12-12 | 韶关学院 | Method for digesting grease through microwave pretreatment and aqueous enzymatic method |
| CN102952624A (en) * | 2012-11-06 | 2013-03-06 | 安徽新世界绿洲茶油有限公司 | Preparation process for strong-flavor tea-seed oil and prepared strong-flavor tea-seed oil |
| CN103205310A (en) * | 2013-05-08 | 2013-07-17 | 长沙理工大学 | Method for sequentially extracting tea saponin and tea seed oil from oil-tea camellia seeds |
| CN103275804A (en) * | 2013-05-10 | 2013-09-04 | 福建省岳秀农林有限公司 | Health protection tea-seed oil and manufacturing technology thereof |
| CN103275804B (en) * | 2013-05-10 | 2015-11-18 | 周萍 | A kind of health care tea-seed oil and reparation technology thereof |
| CN103773591A (en) * | 2014-02-17 | 2014-05-07 | 广西巴马印象生活体验产业有限公司 | Method for extracting tea oil |
| CN104804899A (en) * | 2015-03-31 | 2015-07-29 | 湖南农业大学 | Method for preparing scrubbing solution basic liquid by recycling wastewater produced in oil-refining process with aqueous enzymatic method |
| CN104804899B (en) * | 2015-03-31 | 2016-03-09 | 湖南农业大学 | A kind of method of recycle-water enzyme process oil-producing technique waste water preparing washing liquid basal liquid |
| CN105238551B (en) * | 2015-10-09 | 2018-12-21 | 福建天斯顿生物科技有限公司 | A kind of supercritical CO2The method of abstraction purification camellia seed oil |
| CN105238551A (en) * | 2015-10-09 | 2016-01-13 | 福建天斯顿生物科技有限公司 | Method for supercritical CO2 extraction and purification of camellia seed oil |
| CN106318599A (en) * | 2016-08-31 | 2017-01-11 | 浙江工业大学 | Method for increasing yield of camellia seed oil extracted with aqueous enzymatic method |
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| CN106565812B (en) * | 2016-09-26 | 2018-12-07 | 中国林业科学研究院亚热带林业研究所 | A kind of continuous Extraction solvent high efficiente callback technique of Tea Saponin |
| CN115121378A (en) * | 2017-02-28 | 2022-09-30 | 唐凌霄 | Driven centrifugal unit, manufacturing method and using method thereof and solid-liquid separation equipment thereof |
| CN107183225A (en) * | 2017-05-26 | 2017-09-22 | 钦州市桂耘贸易有限公司 | A kind of anti-oxidant tea oil extract and preparation method thereof |
| CN107334678A (en) * | 2017-07-18 | 2017-11-10 | 安徽省华银茶油有限公司 | A kind of method that antibacterial wet tissue is prepared using camellia seed dregs of rice extract |
| CN107653055A (en) * | 2017-09-29 | 2018-02-02 | 浙江工业大学 | A kind of method of ultrasonic wave added extracting tea seed oil by using enzymatic hydrolysis |
| CN107586597A (en) * | 2017-10-11 | 2018-01-16 | 三江县稻香山茶油生态发展有限公司 | A kind of processing method of tea seed |
| CN107474937A (en) * | 2017-10-13 | 2017-12-15 | 三江县稻香山茶油生态发展有限公司 | A kind of method of Enzymatic Extraction tea-seed oil |
| CN108192718A (en) * | 2018-01-05 | 2018-06-22 | 贵州省香瑞莱香料有限公司 | A kind of method that aqueous enzymatic method extracts seed oil of Moringa oleigera and protein hydrolysate simultaneously |
| CN112972376A (en) * | 2021-03-02 | 2021-06-18 | 浙江亚林生物科技股份有限公司 | Preparation method of active peptide nanoemulsion in camellia seeds |
| CN114794468A (en) * | 2022-04-22 | 2022-07-29 | 贵州山茶产业综合开发有限公司 | In-situ camellia oil emulsion and preparation method and application thereof |
| CN114794468B (en) * | 2022-04-22 | 2023-09-26 | 贵州山茶产业综合开发有限公司 | In-situ camellia oil emulsion and preparation method and application thereof |
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