CN110698879A - Method for extracting natural pigment from mulberry - Google Patents
Method for extracting natural pigment from mulberry Download PDFInfo
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- CN110698879A CN110698879A CN201911094984.3A CN201911094984A CN110698879A CN 110698879 A CN110698879 A CN 110698879A CN 201911094984 A CN201911094984 A CN 201911094984A CN 110698879 A CN110698879 A CN 110698879A
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- mulberry
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- mulberries
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B61/00—Dyes of natural origin prepared from natural sources, e.g. vegetable sources
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B67/00—Influencing the physical, e.g. the dyeing or printing properties of dyestuffs without chemical reactions, e.g. by treating with solvents grinding or grinding assistants, coating of pigments or dyes; Process features in the making of dyestuff preparations; Dyestuff preparations of a special physical nature, e.g. tablets, films
- C09B67/0096—Purification; Precipitation; Filtration
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- Chemical Kinetics & Catalysis (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a method for extracting natural pigment from mulberry, which comprises the following specific steps: adding fresh mulberries into salt water, heating, immersing in deionized water, cleaning, and pre-drying to obtain mulberries with clean surfaces; crushing the dried mulberries by using a wall breaking machine, uniformly mixing the crushed mulberries with cellulase, heating, placing in a dark and light-resistant environment for enzymolysis, and then heating, refluxing and primarily extracting to obtain a mulberry extracting solution; soaking and extracting the mulberry extract for 3-5 times by using an ethanol solution, wherein the volume ratio of the mulberry extract to the ethanol solution is 1: 2, soaking and extracting for 18-20 hours each time, filtering and centrifuging, and then concentrating in vacuum at 60 ℃ until no alcohol smell exists to obtain a mulberry concentrated solution; extracting the mulberry concentrated solution by ultrasonic wave to promote pigment leaching, eluting for 2-3 times by using ethanol solution, collecting and concentrating eluent, and drying the obtained concentrated solution to obtain the natural pigment.
Description
Technical Field
The invention belongs to the technical field of pigment extraction, and relates to a method for extracting natural pigment from mulberry.
Background
The natural pigment is added into food for effectively improving the color, the fragrance and the taste of the food, the edible pigment gradually enters our lives with the continuous improvement of the dietary level of people and the rapid development of the food industry in China, and consumers continuously pursue the pure natural edible pigment with safe eating and no toxic or side effect at present in the situation of advocating natural and healthy lives.
Today, resources and development are highly valued at home and abroad, natural pigment additives are widely used in food industry. Compared with artificially synthesized pigments, the natural pigments have many varieties, are safe, have no side effect, can be obtained from plants, animals and microorganisms, and have abundant varieties. Natural pigments are widely present in the vacuoles of plant flowers, stems and leaves, and fruit cells, which is also one of the important reasons why various fruits and vegetables exhibit different colors.
Mulberries, also known as mulberries, jujubes, mulberries and the like, are mature ears of mulberry trees of the Moraceae plant, are mostly purple or black oval flowers and fruits, have the length of about 1-2.5 cm, are thick and honey in juice, are sweet, sour and fragrant, and are rich in nutritional ingredients. The scientific identification shows that the fresh fruits contain a large amount of free acid and 16 amino acids, and also contain mineral substances and trace elements such as zinc, iron, calcium, manganese and the like which are lacked by human bodies, carotene, fructose, glucose, succinic acid pectin, cellulose and the like, so the fresh fruits are known as third-generation fruits together with sea buckthorn, raspberry and the like. Is listed as one of the agricultural products with homology of medicine and food which are not only food but also medicine by the national ministry of health. The mulberry contains main nutritional components such as moisture, sugar, crude protein, ash, crude fiber, free acid, soluble nitrogen-free substances and the like, and in addition, the mulberry also contains essential amino acids for human bodies, easily absorbed polysaccharide, abundant vitamins, haematochrome and mineral substances such as calcium, iron, zinc, selenium and the like which are deficient in human bodies, has the effects of enhancing immunity, promoting the growth of hematopoietic cells, promoting metabolism and the like, and has abundant nutritional value and certain pharmacological value.
However, the utilization rate of pigment extracted from the mulberries by the existing pigment extraction process is low, the yield of the mulberries is originally lower compared with other fruits, resource waste is caused, and the existing process is complex.
Disclosure of Invention
The invention aims to provide a method for extracting natural pigment from mulberry, which solves the problems of low utilization rate of pigment extracted from mulberry and resource waste in the prior art.
The technical scheme adopted by the invention is that the method for extracting the natural pigment from the mulberry concretely comprises the following steps:
step 1, adding fresh mulberries into salt water, heating to 30-35 ℃, immersing in deionized water, cleaning, and pre-drying to obtain mulberries with clean surfaces;
step 2, crushing the dried mulberries by using a wall breaking machine, uniformly mixing the crushed mulberries with cellulase, heating to keep the temperature at 50-55 ℃, placing in a dark and light-resistant environment, carrying out enzymolysis for 1-2 days, and then carrying out heating reflux primary extraction to obtain a mulberry extracting solution;
and 3, soaking and extracting the mulberry extracting solution for 3-5 times by using an ethanol solution, wherein the volume ratio of the mulberry extracting solution to the ethanol solution is 1: 2, soaking and extracting for 18-20 hours each time, filtering and centrifuging, and then concentrating in vacuum at 60 ℃ until no alcohol smell exists to obtain a mulberry concentrated solution;
and 4, carrying out ultrasonic extraction on the mulberry concentrated solution to promote pigment leaching, eluting for 2-3 times by using an ethanol solution, wherein the flow rate of the eluent is 1-1.5mL/min, collecting and concentrating the eluent, and drying the obtained concentrated solution to obtain the natural pigment.
The invention is also characterized in that:
the cellulase is endoglucanase or exoglucanase.
And 3, soaking and extracting the mulberry extract by using 50-60% ethanol solution with the pH value of 3.2.
And 4, performing ultrasonic extraction on the mulberry concentrated solution to promote pigment leaching, and then eluting for 1 time by using 50-70% ethanol solution with the pH value of 3.5-4.5.
In step 4, vacuum concentration is carried out at 50-55 ℃ by using a flash concentration device.
The invention has the beneficial effects that: the process is simple, the pigment extraction rate is high, the utilization rate of the mulberry is high, the resource waste is greatly reduced, other additives are not added in the extraction process, and the mulberry fruit pigment is green, safe and pollution-free and can be used as an edible pigment.
Detailed Description
The invention relates to a method for extracting natural pigment from mulberry, which comprises the following steps:
step 1, adding fresh mulberries into salt water, heating to 30-35 ℃, immersing in deionized water, cleaning, and pre-drying to obtain mulberries with clean surfaces;
step 2, crushing the dried mulberries by using a wall breaking machine, uniformly mixing the crushed mulberries with cellulase, heating to keep the temperature at 50-55 ℃, placing in a dark and light-resistant environment, carrying out enzymolysis for 1-2 days, and then carrying out heating reflux primary extraction to obtain a mulberry extracting solution;
and 3, soaking and extracting the mulberry extracting solution for 3-5 times by using an ethanol solution, wherein the volume ratio of the mulberry extracting solution to the ethanol solution is 1: 2, soaking and extracting for 18-20 hours each time, filtering and centrifuging, and then concentrating in vacuum at 60 ℃ until no alcohol smell exists to obtain a mulberry concentrated solution;
and 4, carrying out ultrasonic extraction on the mulberry concentrated solution to promote pigment leaching, eluting for 2-3 times by using an ethanol solution, wherein the flow rate of the eluent is 1-1.5mL/min, collecting and concentrating the eluent, and drying the obtained concentrated solution to obtain the natural pigment.
The cellulase is endoglucanase or exoglucanase.
And 3, soaking and extracting the mulberry extract by using 50-60% ethanol solution with the pH value of 3.2.
And 4, performing ultrasonic extraction on the mulberry concentrated solution to promote pigment leaching, and then eluting for 1 time by using 50-70% ethanol solution with the pH value of 3.5-4.5.
In step 4, vacuum concentration is carried out at 50-55 ℃ by using a flash concentration device.
The invention relates to a method for extracting natural pigment from mulberry, which has the advantages that: the method solves the problems of low utilization rate of pigment extracted from the mulberries and resource waste in the prior art, has the advantages of simple pigment extraction process, high pigment extraction rate and high utilization rate of the mulberries, greatly reduces the resource waste, does not add other additives in the extraction process, is green, safe and pollution-free, and can be used as edible pigment.
Claims (5)
1. A method for extracting natural pigment from mulberry is characterized by comprising the following steps:
step 1, adding fresh mulberries into salt water, heating to 30-35 ℃, immersing in deionized water, cleaning, and pre-drying to obtain mulberries with clean surfaces;
step 2, crushing the dried mulberries by using a wall breaking machine, uniformly mixing the crushed mulberries with cellulase, heating to keep the temperature at 50-55 ℃, placing in a dark and light-resistant environment, carrying out enzymolysis for 1-2 days, and then carrying out heating reflux primary extraction to obtain a mulberry extracting solution;
and 3, soaking and extracting the mulberry extracting solution for 3-5 times by using an ethanol solution, wherein the volume ratio of the mulberry extracting solution to the ethanol solution is 1: 2, soaking and extracting for 18-20 hours each time, filtering and centrifuging, and then concentrating in vacuum at 60 ℃ until no alcohol smell exists to obtain a mulberry concentrated solution;
and 4, carrying out ultrasonic extraction on the mulberry concentrated solution to promote pigment leaching, eluting for 2-3 times by using an ethanol solution, wherein the flow rate of the eluent is 1-1.5mL/min, collecting and concentrating the eluent, and drying the obtained concentrated solution to obtain the natural pigment.
2. The method for extracting natural pigment from mulberry according to claim 1, wherein the cellulase is endoglucanase or exoglucanase.
3. The method for extracting natural pigment from mulberry according to claim 1, wherein the mulberry extract is extracted by soaking the mulberry extract in 50% -60% ethanol solution with pH of 3.2 in the step 3.
4. The method for extracting natural pigment from mulberry according to claim 1, wherein the mulberry concentrated solution is subjected to ultrasonic extraction to promote pigment leaching in the step 4, and then is eluted 1 time by using 50-70% ethanol solution with pH of 3.5-4.5.
5. The method for extracting natural pigments from mulberry according to claim 1, wherein the step 4 is performed vacuum concentration at 50-55 ℃ using a flash concentration device.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201911094984.3A CN110698879A (en) | 2019-11-11 | 2019-11-11 | Method for extracting natural pigment from mulberry |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201911094984.3A CN110698879A (en) | 2019-11-11 | 2019-11-11 | Method for extracting natural pigment from mulberry |
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| CN110698879A true CN110698879A (en) | 2020-01-17 |
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| CN201911094984.3A Pending CN110698879A (en) | 2019-11-11 | 2019-11-11 | Method for extracting natural pigment from mulberry |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112111172A (en) * | 2020-09-14 | 2020-12-22 | 苏州森创纺织科技有限公司 | Extraction method of anti-bacterial plant dye combining cellulose mildew and ultrasonic waves |
Citations (7)
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|---|---|---|---|---|
| CN102212053A (en) * | 2011-04-16 | 2011-10-12 | 湖州柳荫生物科技有限公司 | Method for extracting mulberry cyanidin |
| CN104513217A (en) * | 2013-09-26 | 2015-04-15 | 青岛中人智业生物科技有限公司 | Mulberry anthocyanidin extraction technology |
| CN105037575A (en) * | 2015-07-22 | 2015-11-11 | 贵州师范大学 | Extraction method and product of fructus mori polysaccharide |
| CN105061529A (en) * | 2015-08-31 | 2015-11-18 | 桂林茗兴生物科技有限公司 | Extraction process of mulberry anthocyanin |
| CN105385188A (en) * | 2015-12-09 | 2016-03-09 | 重庆三零三科技有限公司 | Preparation method of mulberry red pigment |
| CN106046848A (en) * | 2016-06-06 | 2016-10-26 | 西昌学院 | Method for extracting and purifying anthocyanin from mulberry pomace |
| CN106579443A (en) * | 2016-12-29 | 2017-04-26 | 界首市菁华科技信息咨询服务有限公司 | Method for extracting anthocyanin from mulberries |
-
2019
- 2019-11-11 CN CN201911094984.3A patent/CN110698879A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102212053A (en) * | 2011-04-16 | 2011-10-12 | 湖州柳荫生物科技有限公司 | Method for extracting mulberry cyanidin |
| CN104513217A (en) * | 2013-09-26 | 2015-04-15 | 青岛中人智业生物科技有限公司 | Mulberry anthocyanidin extraction technology |
| CN105037575A (en) * | 2015-07-22 | 2015-11-11 | 贵州师范大学 | Extraction method and product of fructus mori polysaccharide |
| CN105061529A (en) * | 2015-08-31 | 2015-11-18 | 桂林茗兴生物科技有限公司 | Extraction process of mulberry anthocyanin |
| CN105385188A (en) * | 2015-12-09 | 2016-03-09 | 重庆三零三科技有限公司 | Preparation method of mulberry red pigment |
| CN106046848A (en) * | 2016-06-06 | 2016-10-26 | 西昌学院 | Method for extracting and purifying anthocyanin from mulberry pomace |
| CN106579443A (en) * | 2016-12-29 | 2017-04-26 | 界首市菁华科技信息咨询服务有限公司 | Method for extracting anthocyanin from mulberries |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112111172A (en) * | 2020-09-14 | 2020-12-22 | 苏州森创纺织科技有限公司 | Extraction method of anti-bacterial plant dye combining cellulose mildew and ultrasonic waves |
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