CN103059158A - Method for inhibiting foam generation in tea flower polysaccharide extraction - Google Patents

Method for inhibiting foam generation in tea flower polysaccharide extraction Download PDF

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Publication number
CN103059158A
CN103059158A CN2013100333310A CN201310033331A CN103059158A CN 103059158 A CN103059158 A CN 103059158A CN 2013100333310 A CN2013100333310 A CN 2013100333310A CN 201310033331 A CN201310033331 A CN 201310033331A CN 103059158 A CN103059158 A CN 103059158A
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China
Prior art keywords
tea
tea flower
precipitation
polysaccharide
ethanol
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CN2013100333310A
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Chinese (zh)
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杨普香
李文金
江新凤
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JIANGXI SERICULTURE AND TEA RESEARCH INSTITUTE
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JIANGXI SERICULTURE AND TEA RESEARCH INSTITUTE
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Priority to CN2013100333310A priority Critical patent/CN103059158A/en
Publication of CN103059158A publication Critical patent/CN103059158A/en
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  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

The invention discloses a method for inhibiting foam generation in tea flower polysaccharide extraction, and belongs to the field of deep processing of tea resources. The method comprises the steps of tea flower treatment, microwave digestion, centrifugation after filtration, foam inhibition treatment, vacuum concentration, ethanol precipitation, and low-temperature or freeze drying. A foam inhibition technique adopted by the method disclosed by the invention not only can solve the technical problem that concentration cannot be performed as foam is very easy to be generated during concentration due to a great number of tea saponin contained in the tea flower but also can realize effective separation of subsequent ethanol precipitate. The method disclosed by the invention is simple in technique, realizes high extraction rate, does not cause 'the three wastes', causes no pollution, makes a solvent be easily recycled, and has low cost and good economic benefit. A polysaccharide product obtained by the method disclosed by the invention is soluble in cold water and hot water, and not only can be used as an instant health care beverage but also can be used as a health care food additive, so that the application of a tea resource product is widened.

Description

A kind of method that suppresses the generation of Tea Flower polysaccharide leaching process foam
Technical field
The present invention relates to a kind of suds suppressor that method, particularly a kind of the present invention of employing that Tea Flower polysaccharide leaching process foam produces prepare and addition of suppressing not only can suppress a large amount of foams of Tea Flower extract concentration process and produce, but also can make follow-up precipitation reach the foam inhibition technology of best effect.
Background technology
Tea polysaccharide is a kind of acid glycoprotein, and is combined with a large amount of mineral elements, is called the Tea Polysaccharides mixture, referred to as Tea Polysaccharides or tea polysaccharide (Tea Polysaccharide).Tea Flower is the reproductive organ of tea tree, and China's tea place total area is 1,500,000 hectares at present, and year can be produced tree plant flowers more than 1,700,000 tons.Modern study finds that Tea Flower contains the materials such as a large amount of protein, tea polysaccharide, tea-polyphenol.And total sugar content is higher than the coarse tea leaf far away in the Tea Flower.Protein and reducing sugar content are quite high in the camellia, and both sums account for dry matter 45%, even more.Compare with multiple edible plant flowers, Tea Flower has good comprehensive utilization DEVELOPMENT PROSPECT.The effects such as that tea polysaccharide has is hypoglycemic, reducing blood-fat, strengthening immunity, hypotensive, reducing heart rate, increase coronary flow, anticoagulation, antithrombotic and hypoxia tolerance, the discovered in recent years tea polysaccharide also has the effect for the treatment of diabetes.Therefore, take full advantage of the camellia resource, carry out the deep processing development utilization, the product that extracts directly utilizes or is applied to other industries, not only have larger social benefit, and can greatly increase economic efficiency, plucked owing to Tea Flower simultaneously, be conducive to tea tree and nourish and grow, for the increasing yield and improving quality of tealeaves is created good condition.
The solubleness of tea polysaccharide crude product in 85~90 ℃ of hot water is 76%, and tea polysaccharide is insoluble to the organic solvents such as the ethanol, acetone, ethyl acetate, propyl carbinol of high density, and is relatively poor to thermostability.The Tea Flower of at present data record or one-tenth are sampled tea the extracting method of middle tea polysaccharide usually through lixiviate, filtration and centrifugal, vacuum concentration, ethanol precipitation, the technique such as dry, but because saponin content is higher in the Tea Flower, produce a large amount of foams during vacuum concentration, cause concentration process normally to carry out, if use general suds suppressor bring impact can for the ethanol precipitation of back, the generation of impact precipitation with separate.
Summary of the invention
The object of the present invention is to provide a kind of foam inhibition technology of a kind of Tea Flower polysaccharide extraction and purge process.
Technical scheme of the present invention is: a kind of method that suppresses the generation of Tea Flower polysaccharide leaching process foam may further comprise the steps:
(1) Tea Flower is processed and the microwave lixiviate: will pluck fresh Tea Flower tissue mashing, press tree plant flowers 1kg than 14kg water, or pluck tree plant flowers through microwave de-enzyming and dryly pulverize after must doing Tea Flower, by dried tea plant pollen (fresh flower of harvesting through complete, the Tea Flower of baking operation after processing, moisture content 5-7%) 1kg adds distilled water than the ratio of 18kg water, puts into microwave oven, and moderate heat (400W) extracted 25-30 minute, filtered while hot, centrifugal; (2) pressing down bubble processes: get previous step and filter the suds suppressor that the supernatant liquor that obtains after centrifugal adds 0.8-2 ‰, fully mixing leaves standstill slightly; (3) concentrated: as to put into vacuum concentrating apparatus, at pressure 0.09Mpa, temperature 65-75 ℃; Condition under vacuum concentration to 1/8 volume; (4) precipitation: take out concentrated solution, 70% ethanol that in concentrated solution, adds equal volume after the cooling, centrifugal, remove supernatant liquor, in precipitation, add again once 70% ethanol of half volume, centrifugal, 70% ethanol of half volume once in precipitation, adding again, until (after the precipitation process) supernatant liquor is colourless, all supernatant liquors merge, Distillation recovery ethanol.(5) drying: take out 70 ℃ of oven dryings of precipitation and get brown polysaccharide, or lyophilize get yellow polysaccharide the higher fiery drying of microwave make camellia; Camellia directly or after the reprocessing eats.
At described a kind of specific suds suppressor that bubble uses in processing and usage quantity (the compound suds suppressor that described suds suppressor is prepared in the 9:1 ratio as tween 80 and toluylic acid bay alcohol ester that presses down, usage quantity is 0.8-2.0 ‰), as use other suds suppressor or usage quantity is very few or too much all can not reach the desirable bubble effect that presses down, also can affect simultaneously the generation of post precipitation process precipitation.The control of the selection of suds suppressor kind and the control of amount, concentrated solution volume and alcohol concn is the key of successfully extracting polysaccharide in present method.Described suds suppressor is the compound suds suppressor that tween 80 and toluylic acid bay alcohol ester are prepared in the 9:1 ratio, and usage quantity is 0.8-2.0 ‰; Vacuum concentration condition pressure 0.08-0.10Mpa, temperature 65-75 ℃; The concentrated solution volume is about 1/8 of present method institute usage ratio gained vat liquor volume; Alcohol concn is 70%, and usage quantity is for the first time identical with the concentrated solution volume, and the second (three) inferior 1/2 volume uses 2 times at least.The extraction yield that adopts this technique to extract the camellia polysaccharide crude can reach about 10%.
The invention has the advantages that: adopt this to press down the bubble treatment process, solved in the Tea Flower polysaccharide crude extract vacuum Concentrating Process, a large amount of foams produce, concentration process can't normally be carried out, and use conventional suds suppressor to affect the technological difficulties of ethanol precipitate and separate tea polysaccharide, make concentrated vacuum tightness, concentrated speed and the ethanol precipitation of having realized reach best of breed, improved thickening efficiency, improved extraction yield; The polysaccharide that utilizes this technology to extract can be dissolved in cold and hot water, and in the extraction process except having used ethanol and less than 2 ‰ the suds suppressor, do not use any other chemical reagent, the suds suppressor food grade additives of the present invention's preparation is preparation of raw material, extracting method is safe, easy and simple to handle, extraction yield is high, it is easy to foaming to have solved the tea polysaccharide water extract, the technical barrier of the concentrated and precipitate and separate of impact, the extraction time that also solves general extraction process is long, energy consumption is high, use in a large number the problem of chemical reagent.The polysaccharide that extracts is direct-edible, also can be used for processing other health product.
Embodiment
Embodiment 1:
The extraction of tree plant flowers polysaccharide
(1) Tea Flower is processed: pluck the direct tissue mashing of tree plant flowers, or pluck tree plant flowers and pulverize after processing makes dried Tea Flower.
(2) lixiviate: in the ratio adding distilled water of tree plant flowers 1kg than 14kg water, put into microwave oven, moderate heat extracted 25 minutes;
(3) filter: from microwave oven, take out, filtered while hot, filtrate is centrifugal;
(4) pressing down bubble processes: get supernatant liquor and add 0.8 ‰ suds suppressor;
(5) concentrated: as to add the extracting solution of suds suppressor, put into vacuum concentrating apparatus, vacuum concentration to 1/8 volume under the condition of pressure 0.09Mpa, temperature 70 C;
(6) precipitation: take out concentrated solution, 70% ethanol that in concentrated solution, adds equal volume after the cooling, centrifugal, remove supernatant liquor, in precipitation, add again once 70% ethanol of half volume, centrifugal, 70% ethanol of half volume once in precipitation, adding again, until supernatant liquor is colourless, all supernatant liquors merge, Distillation recovery ethanol.
(7) drying: take out precipitation, lyophilize gets yellow polysaccharide.
Embodiment 2:
The extraction of tree plant flowers polysaccharide
(1) Tea Flower is processed: pluck the direct tissue mashing of tree plant flowers, or pluck tree plant flowers and pulverize after processing makes dried Tea Flower.
(2) lixiviate: in the ratio adding distilled water of dried tea plant pollen 1kg than 18kg water, put into microwave oven, moderate heat extracted 30 minutes;
(3) filter: from microwave oven, take out, filtered while hot, filtrate is centrifugal;
(4) pressing down bubble processes: get supernatant liquor and add 2 ‰ suds suppressor;
(5) concentrated: as to add the extracting solution of suds suppressor, put into vacuum concentrating apparatus, vacuum concentration to 1/8 volume under the condition of pressure 0.09Mpa, temperature 70 C;
(6) precipitation: take out concentrated solution, 70% ethanol that in concentrated solution, adds equal volume after the cooling, centrifugal, remove supernatant liquor, in precipitation, add again once 70% ethanol of half volume, centrifugal, 70% ethanol of half volume once in precipitation, adding again, until supernatant liquor is colourless, all supernatant liquors merge, Distillation recovery ethanol.
(7) drying: take out precipitation, cryodrying gets brown polysaccharide.

Claims (1)

1. one kind is suppressed the method that Tea Flower polysaccharide leaching process foam produces, and may further comprise the steps:
(1) Tea Flower is processed and the microwave lixiviate: will pluck fresh Tea Flower tissue mashing, and press tree plant flowers 1kg than 14kg water; Or pluck tree plant flowers through microwave de-enzyming and dryly pulverize after must doing Tea Flower, add distilled water by dried tea plant pollen 1kg than the ratio of 18kg water, put into microwave oven, the moderate heat extraction of 400W 25-30 minute, filtered while hot, centrifugal;
(2) pressing down bubble processes: get previous step and filter the suds suppressor that the supernatant liquor that obtains after centrifugal adds 0.8-2 ‰, fully mixing leaves standstill slightly;
(3) concentrated: as to put into vacuum concentrating apparatus, at pressure 0.09Mpa, temperature 65-75 ℃; Condition under vacuum concentration to 1/8 volume;
(4) precipitation: take out concentrated solution, 70% ethanol that in concentrated solution, adds equal volume after the cooling, centrifugal, remove supernatant liquor, in precipitation, add again once 70% ethanol of half volume, centrifugal, 70% ethanol of half volume once in precipitation, adding again, until the supernatant liquor after the precipitation process is colourless, all supernatant liquors merge, Distillation recovery ethanol;
(5) drying: take out 70 ℃ of oven dryings of precipitation and get brown polysaccharide, or lyophilize get yellow polysaccharide the higher fiery drying of microwave make camellia; Camellia directly or after the reprocessing eats;
Described suds suppressor is the compound suds suppressor that tween 80 and toluylic acid bay alcohol ester are prepared in the 9:1 ratio, and usage quantity is 0.8-2.0 ‰, vacuum concentration condition pressure 0.08-0.10Mpa, temperature 65-75 ℃.
CN2013100333310A 2013-01-29 2013-01-29 Method for inhibiting foam generation in tea flower polysaccharide extraction Pending CN103059158A (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103497257A (en) * 2013-09-27 2014-01-08 福建中烟工业有限责任公司 Tea flower polysaccharide, preparation method and application of polysaccharide to cigarette
CN104877039A (en) * 2015-05-29 2015-09-02 上海师范大学 Method for inhibiting foam generation during vacuum concentration of saponin solution
CN106306175A (en) * 2016-08-22 2017-01-11 四川雅安全义茶树花科技有限公司 Tea-flower functional tea and preparation method thereof
CN109393484A (en) * 2018-12-11 2019-03-01 深圳前海金万鸿实业有限公司 Tea flower processing technology and tea flower product

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH1180203A (en) * 1997-09-02 1999-03-26 Kobe Tennenbutsu Kagaku Kk Preparation of viscous polysaccharide
CN101265302A (en) * 2008-04-29 2008-09-17 武汉大学 Panax japonicus polysaccharides, preparation method and use thereof
CN102030834A (en) * 2009-09-24 2011-04-27 上海新康制药厂 Method for extracting and preparing camellia polysaccharide from camellia and application of camellia polysaccharide

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH1180203A (en) * 1997-09-02 1999-03-26 Kobe Tennenbutsu Kagaku Kk Preparation of viscous polysaccharide
CN101265302A (en) * 2008-04-29 2008-09-17 武汉大学 Panax japonicus polysaccharides, preparation method and use thereof
CN102030834A (en) * 2009-09-24 2011-04-27 上海新康制药厂 Method for extracting and preparing camellia polysaccharide from camellia and application of camellia polysaccharide

Non-Patent Citations (1)

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Title
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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103497257A (en) * 2013-09-27 2014-01-08 福建中烟工业有限责任公司 Tea flower polysaccharide, preparation method and application of polysaccharide to cigarette
CN104877039A (en) * 2015-05-29 2015-09-02 上海师范大学 Method for inhibiting foam generation during vacuum concentration of saponin solution
CN106306175A (en) * 2016-08-22 2017-01-11 四川雅安全义茶树花科技有限公司 Tea-flower functional tea and preparation method thereof
CN109393484A (en) * 2018-12-11 2019-03-01 深圳前海金万鸿实业有限公司 Tea flower processing technology and tea flower product

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Application publication date: 20130424