CN111214562A - Compound traditional Chinese medicine injection for treating goose gout caused by novel goose astrovirus and preparation method thereof - Google Patents
Compound traditional Chinese medicine injection for treating goose gout caused by novel goose astrovirus and preparation method thereof Download PDFInfo
- Publication number
- CN111214562A CN111214562A CN202010232690.9A CN202010232690A CN111214562A CN 111214562 A CN111214562 A CN 111214562A CN 202010232690 A CN202010232690 A CN 202010232690A CN 111214562 A CN111214562 A CN 111214562A
- Authority
- CN
- China
- Prior art keywords
- parts
- filtrate
- goose
- traditional chinese
- chinese medicine
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 241000272814 Anser sp. Species 0.000 title claims abstract description 52
- 239000003814 drug Substances 0.000 title claims abstract description 44
- 239000007924 injection Substances 0.000 title claims abstract description 34
- 238000002347 injection Methods 0.000 title claims abstract description 34
- 201000005569 Gout Diseases 0.000 title claims abstract description 31
- 241000540503 Goose astrovirus Species 0.000 title claims abstract description 29
- 238000002360 preparation method Methods 0.000 title claims abstract description 19
- 150000001875 compounds Chemical class 0.000 title claims abstract description 17
- 241000205585 Aquilegia canadensis Species 0.000 claims abstract description 29
- 241001674044 Blattodea Species 0.000 claims abstract description 22
- 244000292697 Polygonum aviculare Species 0.000 claims abstract description 17
- 235000006386 Polygonum aviculare Nutrition 0.000 claims abstract description 17
- 238000004821 distillation Methods 0.000 claims abstract description 15
- 238000001914 filtration Methods 0.000 claims abstract description 15
- 241001116757 Pyrrosia lingua Species 0.000 claims abstract description 13
- 241000045403 Astragalus propinquus Species 0.000 claims abstract description 12
- 240000004534 Scutellaria baicalensis Species 0.000 claims abstract description 12
- 235000017089 Scutellaria baicalensis Nutrition 0.000 claims abstract description 12
- 235000006533 astragalus Nutrition 0.000 claims abstract description 12
- 239000007788 liquid Substances 0.000 claims abstract description 8
- 239000002994 raw material Substances 0.000 claims abstract description 8
- 230000001954 sterilising effect Effects 0.000 claims abstract description 5
- 240000001972 Gardenia jasminoides Species 0.000 claims abstract 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 98
- 239000000243 solution Substances 0.000 claims description 65
- 239000000706 filtrate Substances 0.000 claims description 50
- 230000002829 reductive effect Effects 0.000 claims description 33
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 31
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 24
- 238000000967 suction filtration Methods 0.000 claims description 23
- 238000003756 stirring Methods 0.000 claims description 20
- 238000001816 cooling Methods 0.000 claims description 14
- 238000002156 mixing Methods 0.000 claims description 14
- WVDDGKGOMKODPV-UHFFFAOYSA-N Benzyl alcohol Chemical compound OCC1=CC=CC=C1 WVDDGKGOMKODPV-UHFFFAOYSA-N 0.000 claims description 12
- 238000007710 freezing Methods 0.000 claims description 12
- 230000008014 freezing Effects 0.000 claims description 12
- 239000011259 mixed solution Substances 0.000 claims description 12
- 238000002791 soaking Methods 0.000 claims description 12
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 11
- 235000003826 Artemisia Nutrition 0.000 claims description 10
- 235000003261 Artemisia vulgaris Nutrition 0.000 claims description 10
- 244000030166 artemisia Species 0.000 claims description 10
- 235000009052 artemisia Nutrition 0.000 claims description 10
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims description 10
- 241000246044 Sophora flavescens Species 0.000 claims description 9
- 229920001131 Pulp (paper) Polymers 0.000 claims description 8
- 238000009835 boiling Methods 0.000 claims description 8
- 239000012153 distilled water Substances 0.000 claims description 8
- 229940079593 drug Drugs 0.000 claims description 8
- 238000010438 heat treatment Methods 0.000 claims description 8
- 239000002244 precipitate Substances 0.000 claims description 8
- 239000000047 product Substances 0.000 claims description 8
- 238000007789 sealing Methods 0.000 claims description 8
- 239000000203 mixture Substances 0.000 claims description 7
- 239000007864 aqueous solution Substances 0.000 claims description 4
- 235000019445 benzyl alcohol Nutrition 0.000 claims description 4
- 238000001704 evaporation Methods 0.000 claims description 4
- 238000011049 filling Methods 0.000 claims description 4
- 238000005057 refrigeration Methods 0.000 claims description 3
- 244000111489 Gardenia augusta Species 0.000 claims description 2
- 235000018958 Gardenia augusta Nutrition 0.000 claims description 2
- 230000001376 precipitating effect Effects 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 14
- 230000003115 biocidal effect Effects 0.000 abstract description 7
- 238000000034 method Methods 0.000 abstract description 4
- 235000008658 Artemisia capillaris Nutrition 0.000 abstract description 2
- 241000092668 Artemisia capillaris Species 0.000 abstract description 2
- 239000000273 veterinary drug Substances 0.000 abstract description 2
- 238000000605 extraction Methods 0.000 abstract 1
- 238000004659 sterilization and disinfection Methods 0.000 abstract 1
- 229940126680 traditional chinese medicines Drugs 0.000 abstract 1
- 201000010099 disease Diseases 0.000 description 16
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 16
- 241000272808 Anser Species 0.000 description 13
- 241000157835 Gardenia Species 0.000 description 8
- LEHOTFFKMJEONL-UHFFFAOYSA-N Uric Acid Chemical compound N1C(=O)NC(=O)C2=C1NC(=O)N2 LEHOTFFKMJEONL-UHFFFAOYSA-N 0.000 description 8
- 208000024891 symptom Diseases 0.000 description 7
- 230000001737 promoting effect Effects 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- 241000894006 Bacteria Species 0.000 description 5
- 239000003242 anti bacterial agent Substances 0.000 description 5
- 229940088710 antibiotic agent Drugs 0.000 description 5
- 210000004369 blood Anatomy 0.000 description 5
- 239000008280 blood Substances 0.000 description 5
- 210000004185 liver Anatomy 0.000 description 5
- 210000004072 lung Anatomy 0.000 description 5
- 241000700605 Viruses Species 0.000 description 4
- 210000000436 anus Anatomy 0.000 description 4
- 238000010255 intramuscular injection Methods 0.000 description 4
- 239000007927 intramuscular injection Substances 0.000 description 4
- 210000001503 joint Anatomy 0.000 description 4
- 230000027939 micturition Effects 0.000 description 4
- 210000002966 serum Anatomy 0.000 description 4
- 230000008961 swelling Effects 0.000 description 4
- 206010011224 Cough Diseases 0.000 description 3
- 206010059866 Drug resistance Diseases 0.000 description 3
- 208000004880 Polyuria Diseases 0.000 description 3
- 229940124350 antibacterial drug Drugs 0.000 description 3
- 235000009508 confectionery Nutrition 0.000 description 3
- 238000003745 diagnosis Methods 0.000 description 3
- 230000035619 diuresis Effects 0.000 description 3
- 238000009313 farming Methods 0.000 description 3
- 210000003746 feather Anatomy 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 244000052769 pathogen Species 0.000 description 3
- 230000001717 pathogenic effect Effects 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- 210000000952 spleen Anatomy 0.000 description 3
- 210000003932 urinary bladder Anatomy 0.000 description 3
- 206010012735 Diarrhoea Diseases 0.000 description 2
- 206010061218 Inflammation Diseases 0.000 description 2
- 206010023126 Jaundice Diseases 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 208000007502 anemia Diseases 0.000 description 2
- 244000309743 astrovirus Species 0.000 description 2
- 230000008602 contraction Effects 0.000 description 2
- 230000034994 death Effects 0.000 description 2
- 230000003467 diminishing effect Effects 0.000 description 2
- 239000003640 drug residue Substances 0.000 description 2
- 206010016256 fatigue Diseases 0.000 description 2
- 210000000232 gallbladder Anatomy 0.000 description 2
- 210000002216 heart Anatomy 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 230000004054 inflammatory process Effects 0.000 description 2
- 210000003734 kidney Anatomy 0.000 description 2
- 230000033001 locomotion Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 244000045947 parasite Species 0.000 description 2
- 231100000915 pathological change Toxicity 0.000 description 2
- 230000036285 pathological change Effects 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- 238000010926 purge Methods 0.000 description 2
- 210000002784 stomach Anatomy 0.000 description 2
- 231100000331 toxic Toxicity 0.000 description 2
- 230000002588 toxic effect Effects 0.000 description 2
- 231100000419 toxicity Toxicity 0.000 description 2
- 230000001988 toxicity Effects 0.000 description 2
- 201000004384 Alopecia Diseases 0.000 description 1
- 206010006895 Cachexia Diseases 0.000 description 1
- 206010008479 Chest Pain Diseases 0.000 description 1
- 241000721047 Danaus plexippus Species 0.000 description 1
- 208000000059 Dyspnea Diseases 0.000 description 1
- 206010013975 Dyspnoeas Diseases 0.000 description 1
- 208000032843 Hemorrhage Diseases 0.000 description 1
- 239000009636 Huang Qi Substances 0.000 description 1
- 208000006083 Hypokinesia Diseases 0.000 description 1
- 206010062717 Increased upper airway secretion Diseases 0.000 description 1
- 206010023232 Joint swelling Diseases 0.000 description 1
- 206010030113 Oedema Diseases 0.000 description 1
- 208000002193 Pain Diseases 0.000 description 1
- 206010033546 Pallor Diseases 0.000 description 1
- 206010033799 Paralysis Diseases 0.000 description 1
- 206010035148 Plague Diseases 0.000 description 1
- 208000003251 Pruritus Diseases 0.000 description 1
- 206010038084 Rectocele Diseases 0.000 description 1
- 241000207929 Scutellaria Species 0.000 description 1
- 206010061577 Ulcer haemorrhage Diseases 0.000 description 1
- 206010047700 Vomiting Diseases 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 230000001133 acceleration Effects 0.000 description 1
- 208000022531 anorexia Diseases 0.000 description 1
- 239000002246 antineoplastic agent Substances 0.000 description 1
- 229940107666 astragalus root Drugs 0.000 description 1
- 210000003323 beak Anatomy 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 210000000941 bile Anatomy 0.000 description 1
- 230000000740 bleeding effect Effects 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 229940044683 chemotherapy drug Drugs 0.000 description 1
- 208000019902 chronic diarrheal disease Diseases 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 206010061428 decreased appetite Diseases 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000008021 deposition Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 241001493065 dsRNA viruses Species 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 208000026500 emaciation Diseases 0.000 description 1
- 244000144992 flock Species 0.000 description 1
- 230000037406 food intake Effects 0.000 description 1
- 235000012631 food intake Nutrition 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 230000003676 hair loss Effects 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000007803 itching Effects 0.000 description 1
- 230000002147 killing effect Effects 0.000 description 1
- 208000030175 lameness Diseases 0.000 description 1
- 210000002429 large intestine Anatomy 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 230000007721 medicinal effect Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 208000026435 phlegm Diseases 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 230000003449 preventive effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 238000010254 subcutaneous injection Methods 0.000 description 1
- 239000007929 subcutaneous injection Substances 0.000 description 1
- 230000009885 systemic effect Effects 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 230000017423 tissue regeneration Effects 0.000 description 1
- 150000007971 urates Chemical group 0.000 description 1
- 210000000626 ureter Anatomy 0.000 description 1
- 229960005486 vaccine Drugs 0.000 description 1
- 210000001835 viscera Anatomy 0.000 description 1
- 230000009278 visceral effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
- A61K36/539—Scutellaria (skullcap)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/63—Arthropods
- A61K35/64—Insects, e.g. bees, wasps or fleas
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/11—Pteridophyta or Filicophyta (ferns)
- A61K36/12—Filicopsida or Pteridopsida
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
- A61K36/282—Artemisia, e.g. wormwood or sagebrush
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/35—Caprifoliaceae (Honeysuckle family)
- A61K36/355—Lonicera (honeysuckle)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/481—Astragalus (milkvetch)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/489—Sophora, e.g. necklacepod or mamani
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/70—Polygonaceae (Buckwheat family), e.g. spineflower or dock
- A61K36/704—Polygonum, e.g. knotweed
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/74—Rubiaceae (Madder family)
- A61K36/744—Gardenia
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0019—Injectable compositions; Intramuscular, intravenous, arterial, subcutaneous administration; Compositions to be administered through the skin in an invasive manner
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/08—Solutions
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P19/00—Drugs for skeletal disorders
- A61P19/06—Antigout agents, e.g. antihyperuricemic or uricosuric agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
- A61P31/14—Antivirals for RNA viruses
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation or decoction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
Landscapes
- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Chemical & Material Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Epidemiology (AREA)
- Engineering & Computer Science (AREA)
- Botany (AREA)
- Mycology (AREA)
- Medical Informatics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Alternative & Traditional Medicine (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Virology (AREA)
- Insects & Arthropods (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Oncology (AREA)
- Dermatology (AREA)
- Neurosurgery (AREA)
- Animal Husbandry (AREA)
- Communicable Diseases (AREA)
- Zoology (AREA)
- Pain & Pain Management (AREA)
- Rheumatology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Physical Education & Sports Medicine (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention belongs to the technical field of veterinary drugs, and particularly relates to a compound traditional Chinese medicine injection for treating goose gout caused by novel goose astrovirus, which is an injection prepared by processing traditional Chinese medicines through processes of extraction, distillation, filtration, concentration, liquid preparation, sterilization and the like, wherein the raw materials of the traditional Chinese medicine injection comprise 3-6 parts of scutellaria baicalensis, 3-6 parts of artemisia capillaris, 10-20 parts of pyrrosia lingua, 8-16 parts of gardenia, 6-12 parts of honeysuckle, 20-40 parts of astragalus mongholicus, 5-10 parts of cockroaches, 1-3 parts of radix sophorae flavescentis and 1-2 parts of polygonum aviculare. The preparation is a pure traditional Chinese medicine preparation, has no antibiotic residue, and has a good treatment effect on goose gout caused by the novel goose astrovirus.
Description
Technical Field
The invention belongs to the technical field of veterinary drugs, and particularly relates to a compound traditional Chinese medicine injection for treating goose gout caused by novel goose astrovirus and a preparation method thereof.
Background
The new goose astrovirus is a tiny and circular virus, and the diameter of the new goose astrovirus is 25-35 nm. Under an electron microscope, the virus has 5 or 6 star-shaped protrusions on the surface, so the virus is called as a star virus. The astrovirus is a non-capsular membrane positive strand RNA virus, can cause gosling gout, and is mainly characterized in that urate and urate crystals are deposited in organs such as viscera, ureter, kidney, joint cavity and the like; the initial diagnosis can be performed according to the age of the disease and clinical symptoms, and the molecular diagnosis is required for the accurate diagnosis.
At present, the disease is widely prevalent in the world, which causes great economic loss to the goose farming industry in the world, and the occurrence and prevalence of the disease become serious day by day. Diseased geese and bacteria-carrying geese are main infection sources of the disease, and have no clinical symptoms and pathological change geese, or have no clinical symptoms and no pathological change negative bacteria-carrying geese, so that the disease is aggravated and the death rate is increased if secondary or concurrent other diseases are caused; the disease has a tendency of rising year by year in China in recent years, becomes one of the most serious diseases harming goose farming, and causes serious economic loss to the goose farming in China.
In recent years, new goose astrovirus has become an important disease affecting the development of the goose industry. After the disease occurs, the mortality of animals can be obviously reduced by adopting antibiotics for treatment, and the incidence of the disease can be reduced to a certain extent by properly adding the antibiotics in the production process; however, with the wide use of a large number of antibacterial drugs, particularly broad-spectrum antibacterial drugs, in the breeding industry, the problem of drug resistance of bacteria to antibacterial drugs is more and more prominent, and meanwhile, research data shows that antibiotics with sub-inhibitory concentration can influence the metabolic process of bacteria to change the toxicity of bacteria or the adaptability of bacteria to the environment and the like. There have been no effective vaccines, chemotherapeutic drugs or other methods reported to date to control and prevent astrovirus infection, which has been a problem that continues to plague the goose industry.
The early stage of the disease is effectively treated by antibiotics, the death can be reduced, common intramuscular or subcutaneous injection needs large dose repeated administration, but the drug resistance is easy to generate, serious drug residue is caused, and the food safety is influenced, so that a drug which can treat goose gout and does not generate antibiotic residue is needed.
Disclosure of Invention
Aiming at the problem that antibiotic residues in meat and eggs are easily caused by the fact that goose gout is treated by antibiotic, the invention provides a non-residue traditional Chinese medicine injection for treating goose gout and a preparation method thereof.
The technical scheme adopted by the invention for solving the technical problems is as follows: a compound traditional Chinese medicine injection for treating goose gout caused by novel goose astrovirus comprises the following traditional Chinese medicine raw materials in parts by weight: 3-10 parts of scutellaria baicalensis, 3-10 parts of artemisia capillaries, 15-25 parts of pyrrosia lingua, 10-20 parts of gardenia, 10-15 parts of honeysuckle, 35-45 parts of astragalus membranaceus, 5-15 parts of cockroaches, 1-5 parts of radix sophorae flavescentis and 1-5 parts of polygonum aviculare.
The compound traditional Chinese medicine injection for treating goose gout caused by the novel goose astrovirus comprises the following traditional Chinese medicine raw materials in parts by weight: 6 parts of scutellaria baicalensis, 6 parts of artemisia capillaries, 20 parts of pyrrosia lingua, 16 parts of cape jasmine, 12 parts of honeysuckle, 40 parts of astragalus mongholicus, 10 parts of cockroaches, 3 parts of sophora flavescens and 2 parts of polygonum aviculare.
The invention also provides a preparation method of the compound traditional Chinese medicine injection for treating goose gout caused by the novel goose astrovirus, which comprises the following steps:
step one, putting honeysuckle into a distillation tank, adding water which is 10 times of the weight of the honeysuckle into the distillation tank, distilling, collecting distillate, adding distilled water which is 1.5 times of the weight of the distillate into the distillate for redistillation, collecting redistillation, placing the redistillation in a refrigeration mode at 0-4 ℃, and preserving honeysuckle residues and aqueous solution after distillation for later use;
step two, respectively crushing cockroaches and polygonum aviculare, uniformly mixing, adding 90-95% ethanol with the weight of 4 times of that of the cockroaches and the polygonum aviculare, soaking for 0.5h, percolating after soaking, collecting percolate, and storing residues after percolating for later use;
putting artemisia capillaries, pyrrosia lingua, astragalus mongholicus, sophora flavescens, gardenia and scutellaria baicalensis into a multifunctional decoction pot, adding water which is 5 times of the total amount of the medicines into the pot, heating to 60 ℃, keeping the temperature for 2 hours at the temperature of 60 ℃, then filtering with gauze, collecting filtrate, and storing filtered residues for later use; carrying out reduced pressure distillation and concentration on the collected filtrate until half of the filtrate is concentrated to obtain a first concentrated solution;
step four, adding water in an amount which is 4 times that of the residues into the residues obtained in the step two and the step three for soaking, combining the residues with the honeysuckle residues and the water solution obtained in the step one, boiling for 0.5h, filtering, collecting the first filtrate, and keeping the filtered residues for later use; adding water 3 times the weight of the medicinal residue into the medicinal residue, boiling for 30min, filtering, collecting the second filtrate, mixing with the first filtrate, mixing, and concentrating under reduced pressure to obtain second concentrated solution;
step five, mixing the concentrated solution I and the concentrated solution II, concentrating the mixture into concentrated juice under reduced pressure, cooling the concentrated juice, pouring the concentrated juice into the cockroach percolate to obtain mixed solution, extracting the mixed solution with alcohol, and concentrating the mixed solution under reduced pressure to one third to obtain pure concentrated liquid medicine;
adding 1 time of distilled water into the pure concentrated liquid medicine, uniformly stirring, adding 0.5% of activated carbon, freezing and standing for 24 hours, performing suction filtration by using a No. 3 filter stick or filter paper pulp, and evaporating and concentrating the filtrate to one fifth;
and seventhly, adding honeysuckle distillate into the concentrated solution obtained in the sixth step, adding 1N sodium hydroxide solution to adjust the pH to about 7, adding 0.1% bone charcoal, sealing, insulating water and heating for 2 hours, adding 1N sodium hydroxide solution after cooling to adjust the pH to 6.5-7.0, freezing and placing for 48 hours, performing suction filtration by using a No. 3 filter stick or filter paper pulp, adding 2% benzyl alcohol into the filtrate obtained by suction filtration, uniformly stirring, freezing and placing for 24 hours, performing suction filtration by using a No. 4 filter stick, filling and sealing the filtrate, and sterilizing to obtain a finished product.
The preparation method of the compound traditional Chinese medicine injection for treating goose gout caused by the novel goose astrovirus comprises the following steps:
(1) adding 95% ethanol into the mixed solution, stirring, adjusting ethanol content to 50%, standing for 12 hr after a large amount of flocculent precipitate is separated out, vacuum filtering, collecting filtrate as first refined ethanol solution, recovering ethanol, and concentrating under reduced pressure to half amount;
(2) pouring the concentrated solution obtained in the step (1) into 95% ethanol with the volume of 1.6-2 times of that of the concentrated solution, continuously stirring, adjusting the alcohol content to 60%, precipitating flocculent precipitate, standing for 24h, performing suction filtration, collecting filtrate as second refined alcohol solution, recovering ethanol, and concentrating under reduced pressure to half amount;
(3) slowly pouring the concentrated solution obtained in the step (2) into 95% ethanol with the alcohol content being 3.2 times that of the concentrated solution, continuously stirring, adjusting the alcohol content to 75%, separating out a paste, standing for 24h, performing suction filtration to obtain a filtrate, namely a third refined alcohol solution, recovering ethanol, performing reduced pressure concentration to one third, volatilizing the ethanol completely, and cooling.
According to the preparation method of the compound traditional Chinese medicine injection for treating goose gout caused by the novel goose astrovirus, the finished product prepared in the seventh step is a brown yellow clear solution, and the pH value is 5.0-6.5.
The scutellaria in the formula is bitter in taste and cold in nature, is a monarch drug in the formula, has the effects of clearing heat, drying dampness, purging intense heat and detoxifying, and can be used for treating symptoms such as chest distress, nausea and vomiting, diarrhea, lung heat cough and the like.
Herba Artemisiae Scopariae, bitter and pungent in taste, slightly cold in nature, enter spleen, stomach, liver, gallbladder channel, and serve as the ministerial drugs of the formula together with folium Pyrrosiae and fructus Gardeniae, has effects of clearing heat and promoting diuresis, promoting bile flow and removing jaundice, and clearing heat and protecting liver, and can be used for treating rheumatism, cold and heat, jaundice, and protecting liver and gallbladder when being combined with fructus Gardeniae.
Pyrrosia lingua, sweet and bitter in taste and slightly cold in nature, enters lung and bladder channels, has the effects of promoting urination, clearing lung heat, relieving cough, cooling blood, stopping bleeding, relieving cough, eliminating phlegm, inhibiting bacteria and resisting inflammation.
Gardenia, bitter in taste and cold in nature, enters heart, lung and triple energizer channels, has the effects of clearing heat and promoting urination, cooling blood and dissipating blood stasis, and can purge fire and remove toxicity when being combined with radix scutellariae and honeysuckle.
The honeysuckle flower is sweet in taste and cold in nature, and has the effects of clearing away heat and toxic materials, diminishing inflammation and relieving swelling.
Astragalus root, radix astragali, sweet in taste and slightly warm in nature, enters spleen and lung channels, and is used together with cockroach in the formula to tonify qi, strengthen superficies, promote urination and promote tissue regeneration, and is used for treating symptoms such as qi deficiency, hypodynamia, chronic diarrhea and rectocele.
The cockroach is salty in taste and cold in nature, enters liver, spleen and kidney channels, and has the effects of promoting blood circulation to remove blood stasis, detoxifying and diminishing swelling, and resolving masses and removing food retention.
Kuh-seng, bitter in taste and cold in nature, enters heart, liver, stomach, large intestine and bladder meridians, and is used together with Biao knotgrass to clear heat and dry dampness, kill parasites and induce diuresis.
The herba polygoni avicularis is bitter in taste and cold in nature, enters bladder channels, and has the effects of promoting urination, treating stranguria, killing parasites and relieving itching.
The invention has the beneficial effects that: the invention is a pure Chinese medicinal preparation, has reasonable compatibility and obvious medicinal effect, and can be used as a special injection preparation for preventing and treating goose gout caused by novel goose astrovirus. The injection has effects of clearing heat, eliminating dampness, inducing diuresis and relieving edema, and has good therapeutic effect on goose gout caused by new goose astrovirus; particularly has obvious effects on the swelling, the broken walking and the difficult walking of the joint type goose toe and leg joint and the visceral gout. Compared with antibiotic medicines, the traditional Chinese medicine composition has the characteristics of pure nature, preventive treatment, no toxic or side effect, no residue, no drug resistance, complete cure and the like, is reasonable in compatibility, safe and effective, treats both principal and secondary aspect of disease, has no recurrence, avoids drug residue for prevention and treatment by antibiotics, ensures food safety, and is suitable for popularization in animal production. Has better curative effect on preventing and treating goose gout caused by the novel goose astrovirus, and has wide market prospect and larger economic benefit.
Detailed Description
In order to well prevent and control the novel goose astrovirus and solve the hidden danger of antibiotic residue caused by the prevention and treatment of the antibiotic, the invention provides a compound traditional Chinese medicine injection for the goose gout caused by the novel goose astrovirus according to the traditional Chinese medicine theory, and the injection can effectively control the goose gout caused by the novel goose astrovirus. The present invention is further illustrated below with reference to examples to better understand the technical solutions of the present invention, and it should be noted that: in the following examples, 10g of each Chinese medicinal material is 1 part in selected amount.
Example 1: the embodiment provides a compound traditional Chinese medicine injection for treating goose gout caused by novel goose astrovirus, which comprises the following raw materials by weight, 60g of scutellaria baicalensis, 60g of artemisia capillaris, 200g of pyrrosia lingua, 160g of gardenia, 120g of honeysuckle, 400g of astragalus mongholicus, 100g of cockroach, 30g of sophora flavescens and 20g of polygonum aviculare.
The preparation method comprises the following steps:
step one, putting 120g of honeysuckle into a distillation retort, adding 1200mL of water into the retort, distilling, collecting distillate, adding 1.5 times of distilled water into the distillate for redistillation, collecting redistillate, refrigerating the redistillate at 0-4 ℃, and preserving distilled honeysuckle residues and aqueous solution for later use;
step two, respectively crushing cockroaches and polygonum aviculare, uniformly mixing, adding 90-95% ethanol with the weight of 4 times of that of the cockroaches and the polygonum aviculare, soaking for 0.5h, percolating after soaking, collecting percolate, and storing residues after percolating for later use;
putting artemisia capillaries, pyrrosia lingua, astragalus mongholicus, sophora flavescens, gardenia and scutellaria baicalensis into a multifunctional decoction pot, adding water with the weight 5 times that of the medicines into the pot, heating to 60 ℃, keeping the temperature for 2 hours at the temperature of 60 ℃, then filtering with gauze, collecting filtrate, and storing filtered residues for later use; carrying out reduced pressure distillation and concentration on the collected filtrate until half of the filtrate is concentrated to obtain a first concentrated solution;
step four, adding water in an amount which is 4 times that of the residue into the residue obtained in the step two and the residue obtained in the step three for soaking, combining the residue with the honeysuckle residue and the water solution obtained in the step one, boiling for 30min, filtering, and collecting the first filtrate; adding water 3 times the weight of the medicinal residue into the medicinal residue, boiling for 30min, filtering, collecting the second filtrate, mixing with the first filtrate, mixing, and concentrating under reduced pressure to obtain second concentrated solution;
step five, combining the concentrated solution I and the concentrated solution II, concentrating the mixture into thick juice under reduced pressure, cooling the thick juice, pouring the mixture into cockroach percolate to obtain mixed solution, adding 95% ethanol into the mixed solution, continuously stirring, adjusting the ethanol content to be 50%, standing for 12h after a large amount of flocculent precipitates are separated out, performing suction filtration, collecting filtrate as first refined alcohol solution, recovering ethanol, and concentrating the filtrate under reduced pressure to half amount;
step six, pouring the concentrated solution obtained in the step five into 95% ethanol which is 1.6-2 times of the concentrated solution, continuously stirring, adjusting the alcohol content to 60%, separating out flocculent precipitate, standing for 24 hours, performing suction filtration, collecting filtrate as second refined alcohol solution, recovering ethanol, and concentrating under reduced pressure to half amount;
and step seven, slowly pouring the concentrated solution obtained in the step six into 95% ethanol in an amount which is 3.2 times that of the concentrated solution, continuously stirring, adjusting the alcohol content to 75%, separating out a paste, standing for 24 hours, performing suction filtration to obtain a filtrate, namely a third refined alcohol solution, recovering ethanol, performing reduced pressure concentration to one third, volatilizing the ethanol completely, and cooling.
Step eight, adding 1 time of distilled water into the pure concentrated liquid medicine, uniformly stirring, adding 0.5% of active carbon, freezing and standing for 24 hours, performing suction filtration by using a No. 3 filter stick or filter paper pulp, and evaporating and concentrating the filtrate to one fifth of the original medicine of the preparation;
and step nine, adding honeysuckle distillate into the concentrated solution, adding 1N sodium hydroxide solution to adjust the pH to about 7, adding 0.1% bone charcoal, sealing, insulating water and heating for 2 hours, cooling, measuring the pH to about 5.5, adding 1N sodium hydroxide solution, adjusting the pH to 6.5-7.0, freezing and placing for 48 hours, performing suction filtration by using a No. 3 filter stick or filter paper pulp, adding 2% benzyl alcohol into the filtrate, uniformly stirring, freezing and placing for 24 hours, performing suction filtration by using a No. 4 filter stick, filling and sealing the filtrate, and sterilizing to obtain a finished product, wherein the finished product is a brown yellow clear solution, and the pH is 5.0-6.5.
Example 2: the embodiment provides a compound traditional Chinese medicine injection for treating goose gout caused by novel goose astrovirus, which comprises the following raw materials, by weight, 30g of scutellaria baicalensis, 100g of artemisia capillaries, 150g of pyrrosia lingua, 200g of gardenia, 100g of honeysuckle, 450g of astragalus mongholicus, 150g of cockroaches, 50g of sophora flavescens and 10g of polygonum aviculare.
The preparation method comprises the following steps:
step one, putting honeysuckle into a distillation tank, adding water which is 10 times of the weight of the honeysuckle into the distillation tank, distilling, collecting distillate, adding distilled water which is 1.5 times of the weight of the distillate into the distillate for redistillation, collecting redistillation, placing the redistillation in a refrigeration mode at 0-4 ℃, and preserving honeysuckle residues and aqueous solution after distillation for later use;
step two, respectively crushing cockroaches and polygonum aviculare, uniformly mixing, adding 90-95% ethanol with the weight of 4 times of that of the cockroaches and the polygonum aviculare, soaking for 0.5h, percolating after soaking, collecting percolate, and storing residues after percolating for later use;
putting artemisia capillaries, pyrrosia lingua, astragalus mongholicus, sophora flavescens, gardenia and scutellaria baicalensis into a multifunctional decoction pot, adding water with the weight 5 times that of the medicines into the pot, heating to 60 ℃, keeping the temperature for 2 hours at the temperature of 60 ℃, then filtering with gauze, collecting filtrate, and storing filtered residues for later use; carrying out reduced pressure distillation and concentration on the collected filtrate until half of the filtrate is concentrated to obtain a first concentrated solution;
step four, adding water in an amount which is 4 times of the total amount of the residues into the residues obtained in the step two and the step three, soaking, combining the residues with the honeysuckle residues and the water solution obtained in the step one, boiling for 30min, filtering, and collecting primary filtrate; adding water 3 times the weight of the medicinal residue into the medicinal residue, boiling for 30min, filtering, collecting the second filtrate, mixing with the first filtrate, mixing, and concentrating under reduced pressure to obtain second concentrated solution;
step five, combining the concentrated solution I and the concentrated solution II, concentrating the mixture into thick juice under reduced pressure, cooling the thick juice, pouring the mixture into cockroach percolate to obtain mixed solution, adding 95% ethanol into the mixed solution, continuously stirring, adjusting the ethanol content to be 50%, standing for 12h after a large amount of flocculent precipitates are separated out, performing suction filtration, collecting filtrate as first refined alcohol solution, recovering ethanol, and concentrating the filtrate under reduced pressure to half amount;
step six, pouring the concentrated solution obtained in the step five into 95% ethanol which is 1.6-2 times of the concentrated solution, continuously stirring, adjusting the alcohol content to 60%, separating out flocculent precipitate, standing for 24 hours, performing suction filtration, collecting filtrate as second refined alcohol solution, recovering ethanol, and concentrating under reduced pressure to half amount;
and step seven, slowly pouring the concentrated solution obtained in the step six into 95% ethanol in an amount which is 3.2 times that of the concentrated solution, continuously stirring, adjusting the alcohol content to 75%, separating out a paste, standing for 24 hours, performing suction filtration to obtain a filtrate, namely a third refined alcohol solution, recovering ethanol, performing reduced pressure concentration to one third, volatilizing the ethanol completely, and cooling.
Step eight, adding 1 time of distilled water into the pure concentrated liquid medicine, uniformly stirring, adding 0.5% of activated carbon, freezing and standing for 24 hours, performing suction filtration by using a No. 3 filter stick or filter paper pulp, and evaporating and concentrating the filtrate to one fifth;
and step nine, adding honeysuckle distillate into the concentrated solution, adding 1N sodium hydroxide solution to adjust the pH to about 7, adding 0.1% bone charcoal, sealing, insulating water and heating for 2 hours, cooling, measuring the pH to about 5.5, adding 1N sodium hydroxide solution, adjusting the pH to 6.5-7.0, freezing and placing for 48 hours, performing suction filtration by using a No. 3 filter stick or filter paper pulp, adding 2% benzyl alcohol into the filtrate, uniformly stirring, freezing and placing for 24 hours, performing suction filtration by using a No. 4 filter stick, filling and sealing the filtrate, and sterilizing to obtain a finished product, wherein the finished product is a brown yellow clear solution, and the pH is 5.0-6.5.
Example 3: the same parts of this embodiment as those of embodiment 1 are not repeated, except that the traditional Chinese medicine injection comprises the following raw materials, by weight, 100g of scutellaria baicalensis, 30g of artemisia capillaries, 250g of pyrrosia lingua, 100g of gardenia, 150g of honeysuckle, 350g of astragalus mongholicus, 50g of cockroach, 10g of sophora flavescens and 50g of polygonum aviculare.
Experimental example: in order to demonstrate the effectiveness of the injectable preparation of the invention, the applicant carried out the following tests:
selecting 20 diseased goslings and 20 diseased adult geese, randomly dividing the test geese into 2 groups, namely a test group and a control group, and ensuring that each group comprises 10 goslings and 10 adult geese.
The experimental group injects the injection according to the method of injecting 2ml per young goose and injecting 5ml per adult goose; the control group was injected with physiological saline according to the method of injecting 2 ml/young goose and 5 ml/adult goose. Blood is collected before and after administration, and the concentration of urate in goose serum is measured before and after administration, and the measurement results are shown in table 1.
As can be seen from Table 1, there was no significant difference in the concentrations of urate in the blood serum of the diseased goose in the test group and the control group before administration, and the concentrations of urate in the blood serum of the diseased goose were both high; after the injection is used, the concentration of the urate in the serum of the diseased goose in a test group is obviously reduced, a control group is not obviously changed, and the test group is obviously reduced compared with the control group (P is less than 0.05), which shows that the injection has good treatment effect on the goose gout.
Clinical application effect
Specific case 1: in 8 months and 10 days in 2017, 6000 laying geese exist in a certain goose farmer in Shangqiu, the geese individually suffer from lassitude, pale beak and pale skin, anemia, loose and fallen feathers, slow movement, lameness, swollen joints of legs and wings, pain feeling caused by touch, loose anus, weak contraction, white excrement discharge and pollution of feathers near the anus, and the goose gout is confirmed to be caused by the novel goose astrovirus through pathogen separation and identification.
The injection is used for intramuscular injection twice a day, 4 ml/time is used for 2 days, the disease condition is controlled on the 3 rd day, and the diseased goose is raised to be normal.
Specific case 2: 26 days in 2019, 4 months, 10000 geese of 6 days old in a certain goose farm in south Yang. On-site observation shows that the sick goose has poor growth, anorexia, lassitude, dyspnea, acceleration and deepening, shows systemic dystrophy and has weight lower than normal value; the anus is loose and weak in contraction, white semi-viscous water-like excrement is usually discharged, a large amount of off-white urate is contained, and white semi-liquid dilute excrement is arranged around the anus; the sick goose likes lying, does not want to launch and does not want to move; gradually emaciation and weakness, loose and disorderly feathers, loss of hair and lusterless hair, anemia. The goose gout caused by the novel goose astrovirus is confirmed and diagnosed through pathogen separation and identification.
The traditional Chinese medicine injection is used for intramuscular injection twice a day, 2 ml/injection is carried out each time, and the disease condition is controlled on the 3 rd day after the injection is used for continuous use for 2 days; after 5 days, the return visit is carried out, the symptoms disappear, and the goose group returns to normal.
Specific case 3: in 2019, 23 days after 6 months, 40000 geese of 65 days old in a certain goose farm at the mouth of the week are diseased, and the diseased geese are difficult to walk, limp and paralyzed in severe cases and cannot walk. Swelling joints, particularly leg joints, can gradually form hard and obviously contoured movable nodules due to different degrees of deposition of urate in joint cavities, and discharge gray-yellow cheese-like urate nodules and hemorrhagic ulcer after the nodules are broken; the movement is difficult, some goose wings and leg joints are deformed obviously and take the squatting or single-limb standing posture; the food intake is greatly reduced, the weight is reduced, and particularly the sick conical geese and the gosling grow slowly. The goose gout caused by the novel goose astrovirus is confirmed and diagnosed through pathogen separation and identification.
The traditional Chinese medicine injection is used for intramuscular injection twice a day, 5 ml/injection is carried out each time, and the disease condition is controlled on the 2 nd day after the injection is used for 2 days; after 4 days, the symptoms disappeared and the goose flocks recovered to normal.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and scope of the present invention are intended to be covered thereby.
Claims (5)
1. A compound traditional Chinese medicine injection for treating goose gout caused by novel goose astrovirus is characterized by comprising the following traditional Chinese medicine raw materials in parts by weight: 3-10 parts of scutellaria baicalensis, 3-10 parts of artemisia capillaries, 15-25 parts of pyrrosia lingua, 10-20 parts of gardenia, 10-15 parts of honeysuckle, 35-45 parts of astragalus membranaceus, 5-15 parts of cockroaches, 1-5 parts of radix sophorae flavescentis and 1-5 parts of polygonum aviculare.
2. The compound traditional Chinese medicine injection for treating goose gout caused by the novel goose astrovirus according to claim 1, which is characterized by comprising the following traditional Chinese medicine raw materials in parts by weight: 6 parts of scutellaria baicalensis, 6 parts of artemisia capillaries, 20 parts of pyrrosia lingua, 16 parts of cape jasmine, 12 parts of honeysuckle, 40 parts of astragalus mongholicus, 10 parts of cockroaches, 3 parts of sophora flavescens and 2 parts of polygonum aviculare.
3. The preparation method of the compound traditional Chinese medicine injection for treating goose gout caused by the novel goose astrovirus as claimed in claim 1 or 2, which comprises the following steps:
step one, putting honeysuckle into a distillation tank, adding water which is 10 times of the weight of the honeysuckle into the distillation tank, distilling, collecting distillate, adding distilled water which is 1.5 times of the weight of the distillate into the distillate for redistillation, collecting redistillation, placing the redistillation in a refrigeration mode at 0-4 ℃, and preserving honeysuckle residues and aqueous solution after distillation for later use;
step two, respectively crushing cockroaches and polygonum aviculare, uniformly mixing, adding 90-95% ethanol with the weight of 4 times of that of the cockroaches and the polygonum aviculare, soaking for 0.5h, percolating after soaking, collecting percolate, and storing residues after percolating for later use;
putting artemisia capillaries, pyrrosia lingua, astragalus mongholicus, sophora flavescens, gardenia and scutellaria baicalensis into a multifunctional decoction pot, adding water which is 5 times of the total amount of the medicines into the pot, heating to 60 ℃, keeping the temperature for 2 hours at the temperature of 60 ℃, then filtering with gauze, collecting filtrate, and storing filtered residues for later use; carrying out reduced pressure distillation and concentration on the collected filtrate until half of the filtrate is concentrated to obtain a first concentrated solution;
step four, adding water in an amount which is 4 times that of the residues into the residues obtained in the step two and the step three for soaking, combining the residues with the honeysuckle residues and the water solution obtained in the step one, boiling for 0.5h, filtering, collecting the first filtrate, and keeping the filtered residues for later use; adding water 3 times the weight of the medicinal residue into the medicinal residue, boiling for 30min, filtering, collecting the second filtrate, mixing with the first filtrate, mixing, and concentrating under reduced pressure to obtain second concentrated solution;
step five, mixing the concentrated solution I and the concentrated solution II, concentrating the mixture into concentrated juice under reduced pressure, cooling the concentrated juice, pouring the concentrated juice into the cockroach percolate to obtain mixed solution, extracting the mixed solution with alcohol, and concentrating the mixed solution under reduced pressure to one third to obtain pure concentrated liquid medicine;
adding 1 time of distilled water into the pure concentrated liquid medicine, uniformly stirring, adding 0.5% of activated carbon, freezing and standing for 24 hours, performing suction filtration by using a No. 3 filter stick or filter paper pulp, and evaporating and concentrating the filtrate to one fifth;
seventhly, adding honeysuckle flower distillate into the concentrated solution obtained in the sixth step, adding 1N sodium hydroxide solution to adjust the pH to about 7, adding 0.1% bone charcoal, sealing, heating in a water-proof manner for 2 hours, cooling, adding 1N sodium hydroxide solution, adjusting the pH to 6.5-7.0, standing for 48 hours in a freezing manner, performing suction filtration by using a No. 3 filter stick or filter paper pulp, adding 2% benzyl alcohol into the filtrate, stirring uniformly, standing for 24 hours in a freezing manner, performing suction filtration by using a No. four filter stick, filling and sealing the filtrate, and sterilizing to obtain a finished product.
4. The preparation method of the compound traditional Chinese medicine injection for treating goose gout caused by the novel goose astrovirus according to claim 3,
(1) adding 95% ethanol into the mixed solution, stirring, adjusting ethanol content to 50%, standing for 12 hr after a large amount of flocculent precipitate is separated out, vacuum filtering, collecting filtrate as first refined ethanol solution, recovering ethanol, and concentrating under reduced pressure to half amount;
(2) pouring the concentrated solution obtained in the step (1) into 95% ethanol with the volume of 1.6-2 times of that of the concentrated solution, continuously stirring, adjusting the alcohol content to 60%, precipitating flocculent precipitate, standing for 24h, performing suction filtration, collecting filtrate as second refined alcohol solution, recovering ethanol, and concentrating under reduced pressure to half amount;
(3) slowly pouring the concentrated solution obtained in the step (2) into 95% ethanol with the alcohol content being 3.2 times that of the concentrated solution, continuously stirring, adjusting the alcohol content to 75%, separating out a paste, standing for 24h, performing suction filtration to obtain a filtrate, namely a third refined alcohol solution, recovering ethanol, performing reduced pressure concentration to one third, volatilizing the ethanol completely, and cooling.
5. The preparation method of the compound traditional Chinese medicine injection for treating goose gout caused by the novel goose astrovirus according to claim 3, wherein a finished product prepared in the seventh step is a brownish yellow clear solution, and the pH value is 5.0-6.5.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010232690.9A CN111214562A (en) | 2020-03-28 | 2020-03-28 | Compound traditional Chinese medicine injection for treating goose gout caused by novel goose astrovirus and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010232690.9A CN111214562A (en) | 2020-03-28 | 2020-03-28 | Compound traditional Chinese medicine injection for treating goose gout caused by novel goose astrovirus and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111214562A true CN111214562A (en) | 2020-06-02 |
Family
ID=70813354
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010232690.9A Withdrawn CN111214562A (en) | 2020-03-28 | 2020-03-28 | Compound traditional Chinese medicine injection for treating goose gout caused by novel goose astrovirus and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111214562A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111494485A (en) * | 2020-05-29 | 2020-08-07 | 仕裕(焦作)中药生物科技有限公司 | Compound traditional Chinese medicine injection for treating goose gout caused by novel goose astrovirus and preparation method thereof |
-
2020
- 2020-03-28 CN CN202010232690.9A patent/CN111214562A/en not_active Withdrawn
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111494485A (en) * | 2020-05-29 | 2020-08-07 | 仕裕(焦作)中药生物科技有限公司 | Compound traditional Chinese medicine injection for treating goose gout caused by novel goose astrovirus and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103417654A (en) | Traditional Chinese medicine composition for preventing and controlling poultry coccidiosis and preparation method of same | |
| CN111214562A (en) | Compound traditional Chinese medicine injection for treating goose gout caused by novel goose astrovirus and preparation method thereof | |
| CN111494485A (en) | Compound traditional Chinese medicine injection for treating goose gout caused by novel goose astrovirus and preparation method thereof | |
| CN114886961A (en) | Traditional Chinese medicine composition for treating/preventing avian gout and preparation method and application thereof | |
| CN104547186A (en) | Compound fermented type traditional Chinese medicine for preventing and treating fowl cholera and preparation method of compound fermented type traditional Chinese medicine | |
| CN102106999B (en) | A kind of compound Chinese medicinal preparation for the treatment of dysphagia after apoplexy and preparation method thereof | |
| CN107397886A (en) | Chinese medicine composition for preventing and treating piglet red dysentery and its preparation method and application | |
| CN109091393B (en) | Liposome microcapsule for preventing and treating pediatric epilepsy | |
| CN115350233B (en) | Oral liquid for protecting livers of livestock and poultry and preparation method thereof | |
| CN110302326A (en) | A kind of Chinese herbal compound preparation and preparation method for preventing and treating Trichomonad of dove | |
| CN103690771B (en) | Be used for the treatment of medicine of lamb colibacillosis and preparation method thereof | |
| CN117462620B (en) | Traditional Chinese medicine formula for treating lung cancer and preparation method and application thereof | |
| CN103768153A (en) | Traditional Chinese medicine composition for treating air sacculitis of poultry and preparation method thereof | |
| CN109091633B (en) | Composition for treating recurrent respiratory tract infection of children and preparation method and application thereof | |
| CN115607614B (en) | Traditional Chinese medicine composition for treating colibacillosis of livestock and poultry and preparation method thereof | |
| CN116036174B (en) | Medicine for treating pulmonary nodules and preparation method thereof | |
| CN111375028B (en) | Preparation and use method of compound traditional Chinese medicine oral liquid for preventing and treating gosling gout | |
| CN106620211A (en) | Traditional Chinese medicine composition for treating pigeon chlamydiosis | |
| CN101045116A (en) | Traditional Chinese medicine for treating cholecystitis | |
| CN105497459A (en) | Traditional Chinese medicine preparation for treating woman leukorrheal diseases as well as preparation method and application of traditional Chinese medicine preparation | |
| CN105381320A (en) | Traditional Chinese medicine for treating chronic bronchitis caused when phlegm heat is accumulated in lungs and preparation method thereof | |
| CN104940373A (en) | Traditional Chinese medicine oral solution for treating pawn eye-rot disease and preparation method thereof | |
| CN105012761A (en) | Traditional Chinese medicine composition for treating urinary tract infection | |
| CN104491070A (en) | Traditional Chinese medicinal extract compound used for preventing and treating chicken coccocidiosis and production method thereof | |
| CN104208136A (en) | Preparation process and using method of sophora alopecuroide alkaloid dairy cow breast injectant |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| WW01 | Invention patent application withdrawn after publication | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20200602 |