CN105766802B - The artificial fecundation method of rhizophagus grandis - Google Patents
The artificial fecundation method of rhizophagus grandis Download PDFInfo
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- CN105766802B CN105766802B CN201610133148.1A CN201610133148A CN105766802B CN 105766802 B CN105766802 B CN 105766802B CN 201610133148 A CN201610133148 A CN 201610133148A CN 105766802 B CN105766802 B CN 105766802B
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- 241000452292 Rhizophagus grandis Species 0.000 title claims abstract description 53
- 238000000034 method Methods 0.000 title claims description 47
- 241001300233 Dendroctonus valens Species 0.000 claims abstract description 15
- 241000351396 Picea asperata Species 0.000 claims abstract description 4
- 230000017448 oviposition Effects 0.000 claims description 57
- 235000013601 eggs Nutrition 0.000 claims description 31
- 102000002322 Egg Proteins Human genes 0.000 claims description 19
- 108010000912 Egg Proteins Proteins 0.000 claims description 19
- 210000004681 ovum Anatomy 0.000 claims description 19
- 241000382353 Pupa Species 0.000 claims description 15
- 230000012447 hatching Effects 0.000 claims description 8
- 239000004576 sand Substances 0.000 claims description 8
- 241000238631 Hexapoda Species 0.000 claims description 7
- 241001415867 Tragopan caboti Species 0.000 claims description 7
- 230000032683 aging Effects 0.000 claims description 6
- 238000004659 sterilization and disinfection Methods 0.000 claims description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 4
- 241001313742 Callosobruchus chinensis Species 0.000 claims description 3
- 241000254154 Sitophilus zeamais Species 0.000 claims description 3
- 241000894006 Bacteria Species 0.000 claims 1
- 238000009395 breeding Methods 0.000 abstract description 7
- 230000001488 breeding effect Effects 0.000 abstract description 7
- 241000218652 Larix Species 0.000 abstract description 3
- 238000005516 engineering process Methods 0.000 abstract description 3
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 235000005590 Larix decidua Nutrition 0.000 abstract description 2
- 235000005205 Pinus Nutrition 0.000 abstract description 2
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- 241000218683 Pseudotsuga Species 0.000 abstract description 2
- 230000000266 injurious effect Effects 0.000 abstract description 2
- 230000002265 prevention Effects 0.000 abstract description 2
- 241000894007 species Species 0.000 abstract description 2
- 239000000126 substance Substances 0.000 abstract description 2
- 235000014466 Douglas bleu Nutrition 0.000 abstract 1
- 235000005386 Pseudotsuga menziesii var menziesii Nutrition 0.000 abstract 1
- 241000607479 Yersinia pestis Species 0.000 description 12
- 239000000463 material Substances 0.000 description 9
- 230000035929 gnawing Effects 0.000 description 8
- 241001300246 Dendroctonus micans Species 0.000 description 6
- 239000004793 Polystyrene Substances 0.000 description 4
- 229920002223 polystyrene Polymers 0.000 description 4
- 230000004083 survival effect Effects 0.000 description 4
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 3
- 241000254173 Coleoptera Species 0.000 description 3
- 235000011610 Pinus tabuliformis Nutrition 0.000 description 3
- 241000018651 Pinus tabuliformis Species 0.000 description 3
- 230000000384 rearing effect Effects 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- 244000050510 Cunninghamia lanceolata Species 0.000 description 2
- 244000035744 Hura crepitans Species 0.000 description 2
- 241000254109 Tenebrio molitor Species 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
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- 238000012549 training Methods 0.000 description 2
- 241000218642 Abies Species 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 241001484296 Monotomidae Species 0.000 description 1
- 241000218657 Picea Species 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 241000545593 Scolytinae Species 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- 229930003270 Vitamin B Natural products 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 229960005070 ascorbic acid Drugs 0.000 description 1
- 235000010323 ascorbic acid Nutrition 0.000 description 1
- 239000011668 ascorbic acid Substances 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 244000144987 brood Species 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 230000002045 lasting effect Effects 0.000 description 1
- 231100000518 lethal Toxicity 0.000 description 1
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- 230000007774 longterm Effects 0.000 description 1
- 230000003020 moisturizing effect Effects 0.000 description 1
- 239000000123 paper Substances 0.000 description 1
- 239000003415 peat Substances 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 239000001739 pinus spp. Substances 0.000 description 1
- 230000019617 pupation Effects 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 238000012502 risk assessment Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 235000012424 soybean oil Nutrition 0.000 description 1
- 239000003549 soybean oil Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 235000019156 vitamin B Nutrition 0.000 description 1
- 239000011720 vitamin B Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Feed For Specific Animals (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
Red turpentine beetle is one of the important injurious forest-insect for invading China, various Pinus tree species and dragon spruce, Douglas fir, fir and the larch etc. caused harm in its distribution, having the characteristics that cause harm, hidden, breeding amount is big, is not easy to be found, and physical control and chemical prevention are all relatively difficult.Its harm is controlled using the natural enemy rhizophagus grandis of red turpentine beetle, there is important practice significance in production.The present invention is studied by the indoor artificial propagation to rhizophagus grandis, is formd a set of effective rhizophagus grandis production technology, is improved the efficiency of rhizophagus grandis artificial propagation.
Description
Technical field
The present invention relates to a kind of methods of artificial propagation rhizophagus grandis, belong to forestry biological pest control technology.
Background technique
Red turpentine beetle (DendroctonusvalensLeConte) (coleoptera Coleoptera: Scolytidae
Scolytidae) source area is the north America regions such as America & Canada, is one of the important injurious forest-insect for invading China.Its conduct
A kind of secondary insect, various Pinus (Pinus spp.) tree species and dragon spruce (Picea), Huang almost caused harm in its distribution
China fir (Pseudotsuga), fir (Abies) and larch (Larix) etc..
It is evaluated by pest risk analysis (PRA) method, red turpentine beetle has in the forest that China belongs to highly dangerous
Evil biology.52.6 ten thousand hm in China2Seriously occur to Chinese pine (Pinustabulaeformis), lethal 35,000,000 plants of Chinese pines,
2,500,000,000 yuan are lost, No. second killer of China forest is had become.
The harm characteristics of red turpentine beetle are its hidden sexual lifves, and the adult overwhelming majority is all below butt 1m
Intrusion, larva live under bark, in bast cluster feeding, form the common tunnel of sheet.When its tunnel is connected with each other, ring
When around trunk one week, the conducting tissue of trees is cut off and destroys, and causes trees dead rapidly.Red turpentine beetle is passed to China
Since, great changes have occurred in life habit, and the individual of 70%-80% pierces root harm, this allows for preventing and treating this evil
Worm is more difficult.The method of current China's prevention and treatment red turpentine beetle is mainly cleared up brood tree and is fumigated with chemical pesticide, these
Although method can play some control effects, time-consuming, at high cost, environment is polluted, cannot fundamentally reach complete
Control the purpose of pest.From the point of view of the theory of the viewpoint and biological control of the ecological balance and sustainable control, to solve external
The problem of invasive pest, it is necessary to its natural enemy is introduced from foreign countries, carries out biological control using natural enemy, natural enemy is made to base oneself upon colonization, into
And expand its population quantity, reach the lasting control complete and long-term to pest.
Rhizophagus grandis (RhizophagusgrandisGyllenhal) category coleoptera Sha La first section (Coleoptera:
It Rhizophagidae), is the Natural Enemies of Insect Pests of red turpentine beetle, adult and larva can prey on red turpentine beetle
Ovum, larva and pupa play an important role in the population quantity of this important pests of Natural regulation.The Belgium in Europe, method
The states such as state, Britain, Georgia have conducted extensive research and apply to using large gnawing wax shell control D. micans, the natural enemy 20
The sixties in century is taken back from Siberia in Georgia first and is raised on dragon spruce trunk, and the master of pest is then released into
Want spot (Kobakhidze et al, 1965)；Later and respectively in Belgium, French (1978), Britain (1983) and soil
Ear its carried out mass propagation and release.Release 6.7 ten thousand large gnawing wax shells between French 1983-1989 altogether, Britain is from 1984-
Nineteen ninety-five releases 15.6 ten thousand large gnawing wax shells altogether.In France, large gnawing wax shell woodland colonizes rate and has reached 75% (Gregoire
Et al, 1989), Britain is after large gnawing wax shell discharges 3-4, woodland colonize rate be more than 80% (Evans and King,
1989) success, is successively obtained.
Mass rearing natural enemy is the precondition for introducing external natural enemy and carrying out biological control of insect pests.It is more by entomologists
The research and practice in year, the mass propagation method for breeding of large gnawing wax shell are mature on the whole, and various countries mainly use two methods: grafting method
With accurate method for breeding.
The program of grafting method: (1) setting section and connect pest, and D. micans female adult pest is accessed high 0.85-1m, diameter l0-
The fresh China fir section (its is enabled freely to pierce or be manually open) of 20cm, the juggle for connecting pest is placed on steelframe.Below steelframe
Connection is used to collect the funnel of propupa, and the tray of peat or wet sand equipped with 1-3cm thickness is placed in funnel lower end.By 40-45
It accesses pairs of large gnawing wax shell adult when making large small moth larvae development in juggle to 2-3 age.(2) connect rhizophagus grandis at
Worm entered pairs of rhizophagus grandis adult in D. micans nest from the triangle osculum excavated on bark with 2-10 days.Training
After supporting 25-27 days, juggle is placed vertically on steelframe, mature larva is allowed to deviate from naturally.(3) pupa and adult culture, will deviate from
The propupa of tree section is put into the sandbox containing certain moisture Adult Development after .40 days and completes, they are counted and are refrigerated.
Accurate method for breeding including the following steps: (1) Adult worms producting eggs, in round polystyrene box (diameter 50mm, height
In 25mm), it is packed into the coarse grain Cortex Cunninghamiae Lanceolatae of half box moisturizing, 20 2-5 age D. micans larvas is put into, pairs of rhizophagus grandis is added
Adult, adds large gnawing wax shell oviposition stimulant, and upper one fritter bark of cover is cultivated 20 days at 20 DEG C, checks the children of hatching
Worm.(2) above-mentioned larva is gone to and is with step outside, in intermediate large full of broken sawdust and food by the larva raising in large,
It is cultivated at 20 DEG C about 40 days, mature larva can be climbed out of and be fallen in external stairs, to pick be put into emergence box in time at this time.
(3) pupa and adult training method are identical as grafting method.
Currently, China's precision method for breeding breeding rhizophagus grandis whole success rate be not it is very high, still have and further change
Into necessity of its propagation method.
Summary of the invention
The present invention is effectively shortened egg-laying time by adjusting the stocking density suitable with screening rhizophagus grandis adult,
Time of the adult in original matrix is shortened simultaneously, reduces adult mortality, forms suitable for biological control rhizophagus grandis
Production technology.
The present invention provides a kind of artificial fecundation method of rhizophagus grandis, in terms of the floor space for container of laying eggs, with 38-64 it is right/
100cm2Stocking density, be positioned in oviposition container to the female male imago of rhizophagus grandis, and add feed, adult is made to lay eggs
It lays eggs in container.
Further, in terms of the floor space for container of laying eggs, when ovum grain number amount reaches 110-180/100cm in oviposition container2
Density when, choose adult.It is preferred that the adult chosen can be put into new oviposition container, continue to feed in the same way
Oviposition.
Further, the feed is other elder brothers that can eat of natural host, Hosts, rhizophagus grandis of rhizophagus grandis
Worm or man-made feeds, feed dosage are one day amount of rhizophagus grandis adult in oviposition container, replace fresh feed daily.
The present invention is described in detail below.
Material therefor is the common material of this field rhizophagus grandis artificial propagation in rhizophagus grandis artificial fecundation method of the present invention
Material, including rhizophagus grandis adult, oviposition container, larva raising container, emergence container, sawdust, husky, feed of pupating.
Wherein, rhizophagus grandis adult, which can be, is obtained with accurate method for breeding or grafting method culture, can also be commercially available.
It will be understood by those skilled in the art that oviposition container, can be cross section is arbitrary shape, circular, pros
Shape or rectangular culture box, further preferably round culture box.The height of oviposition container is at least 0.5cm, preferably at least
For 0.8cm.
In an embodiment of the invention, when cultivating box using circle, size can be diameter × height=10cm
× 1cm perhaps specification ruler diameter × height=6.5cm × 1.7cm or diameter × height=5cm × 2.5cm etc. commonly used in the art
It is very little.
It will be understood by those skilled in the art that larva raising container and emergence container, can be cross section is arbitrary shape,
The box of circular, square or rectangular, further preferably rectangular box.The height of larva raising container and emergence container
At least 2cm.
In an embodiment of the invention, larva raising container and emergence containers size can be identical, and size can
Think length × width × height=18cm × 10cm × 4cm perhaps length × width × height=13.5cm × 7.5cm × 2.2cm or long ×
The specifications commonly used in the art such as width × height=13.5cm × 7.5cm × 5cm.
The oviposition container, larva raising container and emergence container can be made of material commonly used in the art, such as glass, modeling
Material, resin, paper etc..It is preferred that the oviposition container, larva raising container and emergence container are all made of polystyrene material.
Sawdust crushes after taking trunk bast to dry, and stores under room temperature, dark surrounds.
It will be understood by those skilled in the art that the sand of pupating can be selected from but not limited to river sand or sea sand, preferably sea sand.
The feed can be the natural host of rhizophagus grandis, such as adult, the children of red turpentine beetle or D. micans
Worm, pupa and/or ovum；Or the adult, larva, pupa and/or ovum of Hosts of rhizophagus grandis, such as Cabot's Tragopan or calliphorid etc.；Or
Adult, larva, pupa and/or the ovum of other insects that rhizophagus grandis can be eaten, such as sitophilus zea-mais or Callosobruchus chinensis etc.；Or artificial feeding
Material.
The composition of man-made feeds can be for example, be added 3g bark powder, 3g yeast powder, 6g Hosts tissue in every 40ml water
Homogenate, 4g cholesterol, 1.5g sucrose, 1.5g agar, 1ml soybean oil, 0.1g vitamin B, 0.05g ascorbic acid, 0.02g benzene first
Acid.
Preferred feed is natural host, Hosts or other insects of rhizophagus grandis；Most preferably red turpentine beetle,
D. micans, Cabot's Tragopan, calliphorid, sitophilus zea-mais or the adult of Callosobruchus chinensis, larva, pupa and/or ovum.
The health or disinfection control of material therefor, the custom requirements all in accordance with the raising of this field rhizophagus grandis carry out.For example,
Each raising container and tweezers etc. is sterilized using preceding with 75% alcohol wipe.Sawdust mixed with aqua sterilisa it is wet, with mix with.It pupates husky mistake
After sieve, through high-temperature sterilization.Matrix and food will guarantee hygienic, clean and fresh.
It is sterilized 2 hours it is preferred that pupating husky 150 DEG C, mixes wet spare, pupating after further preferably sterilizing with aqua sterilisa later
Sand is used further to pupate after mixing wet placement 1d with aqua sterilisa.
It will be understood by those skilled in the art that feeding environment common and conventional in the rhizophagus grandis artificial propagation of this field is all
Suitable for the present invention, including temperature, humidity and illumination condition.
It is preferred that the environment temperature of rhizophagus grandis artificial propagation is 18-25 DEG C, preferably 19-23 DEG C, more preferably 19-20
℃。
Preferred ambient humidity is 65-90%, preferably 70-75%.
It is preferred that feeding environment is protected from light dark.
It will be understood by those skilled in the art that the reproductive stage in rhizophagus grandis artificial fecundation method of the invention is ability
The stage of domain rhizophagus grandis artificial propagation routine, including Adult worms producting eggs, larva it is aging, pupate and sprout wings.
1, the Adult worms producting eggs stage: in terms of the floor space for container of laying eggs, with 38-64 it is right/100cm2Stocking density, laying eggs
It is positioned in container to the female male imago of rhizophagus grandis, and adds feed, adult is made to lay eggs in oviposition container.
It is preferred that the stocking density be 42-60 it is right/100cm2, further preferably 45-56 is right/100cm2, more preferably
48-53 is right/100cm2。
Laying sawdust in oviposition container, the height of sawdust are at least 0.5cm.
Further, in terms of the floor space for container of laying eggs, when ovum grain number amount reaches 110-180/100cm in oviposition container2
Density when, choose adult.
It is preferred that the ovum grain number amount reaches 120-170/100cm2, further preferably reach 130-150/100cm2When,
Choose adult.
The feed is other insects that the natural host, Hosts, rhizophagus grandis of rhizophagus grandis can be eaten, or artificial
Feed, preferably feed dosage are one day amount of rhizophagus grandis adult in oviposition container, replace fresh feed daily.
It is preferred that the adult chosen can be put into new oviposition container, continue feeding oviposition in the same way.
2, larva is aging: counting since egg hatching, after about 18 days, after usual 18~25 days, larva is aging.
It is preferred that allowing larva feeding in oviposition container to be then moved out to 2 ages after egg hatching is at larva in oviposition container
It is placed into larva raising container and continues to feed.
It is preferred that the dosage of feed is one day amount of larva in box in oviposition container, fresh feed is replaced daily.
It is preferred that the long larva to 2 ages is transferred to the density raised in larva raising container is, with larva raising container bottoms
Product meter, 200-350 larva/100cm2, further preferably 250-300 larva/100cm2.By by worm age consistent children
Worm is put together raising with above-mentioned stocking density, advantageously ensures that larva worm age is consistent, improves larva survival rate.
By taking an embodiment of the invention as an example, diameter × height=10cm × 1cm oviposition box, length × width × height are used
When=18cm × 10cm × 4cm larva raising box, a larva raising box can put 2 instar larvaes of 4-6 oviposition box, preferably
Put 2 instar larvaes of 5 oviposition boxes.
After larva moves to the feeding of larva raising container, fresh feed is preferably replaced daily.
3, pupate and sprout wings: after larva is aging, acquisition mature larva, which is put into be covered with, pupates in husky emergence container, is changed
Pupa and emergence.
In a preferred embodiment of the present invention, using diameter × height=10cm × 1cm oviposition box, grow × wide ×
Height=18cm × 10cm × 4cm larva raising box and emergence box.
By 30-50 to the female male imago pair of rhizophagus grandis, preferably 35-45 pairs, most preferably 40 pairs introduce oviposition box, and add
Feed makes its oviposition.It is preferred that replacing fresh feed daily.
When ovum grain number amount is 90~140, preferably 100~130 in box of laying eggs, choose adult.It is preferred that choose at
Worm is put into new oviposition box, continues feeding oviposition in the same way.
Feed dosage is one day amount of adult in box, such as: daily 1-4 natural hosts or 2-3 Hosts,
Preferably daily 2 Cabot's Tragopan pupas or 1 red turpentine beetle larva.
After egg hatching is larva in oviposition box, its feeding in oviposition box is allowed then to be moved out being placed into larva to 2 ages
Continue to feed in raising box.Feed is preferably daily 2-8 natural host or 3-5 Hosts in oviposition box, more preferably
Daily 4 Cabot's Tragopan pupas, up to larva to 2 ages.
1 larva raising box puts the larva of 4~6 oviposition boxes, continues to feed, replaces fresh feed daily.
Advantages of the present invention:
The present invention effectively shortens egg-laying time by the suitable stocking density of selection rhizophagus grandis adult.
Further, the present invention originates in ovum container by reasonable time point removing adult, changes to new oviposition container,
Time of the adult in former base matter is reduced, the death rate of adult is reduced.
Further, the present invention is by shifting to larva raising container for 2 instar larvaes, and controls larva raising density, guarantees
Larva worm age it is consistent, improve reproductive efficiency.
Detailed description of the invention
Egg laying amount changes over time situation map under Fig. 1 difference stocking density
The eggs on average amount statistical chart of every female adult under Fig. 2 difference stocking density
Adult mortality statistical chart under Fig. 3 difference stocking density
Specific embodiment
The Adult worms producting eggs of 1 rhizophagus grandis of embodiment
Room temperature is 19 DEG C, humidity 75%.
Put 2/3 sawdust in round culture box (diameter × height=10cm × 1cm), sawdust mixed with distilled water it is wet, with
Until hand can not squeeze water droplet.80 rhizophagus grandis adults (40 pairs of female male imagos to) and 1 red turpentine beetle are placed in sawdust
Larva covers box cover, is put in dark place, replaces fresh host daily.
It is the ovum oblong of rhizophagus grandis, white, translucent, less than 2mm, ovum number is counted daily, when ovum grain in culture box
When quantity is 100 or so, adult is chosen to the culture box renewed.
Using identical rearing conditions and method, stocking density is adjusted, 20 pairs of female male imagos of rhizophagus grandis are set again respectively
To group, 30 pairs of female male imagos of rhizophagus grandis are to group, and 50 pairs of female male imagos of rhizophagus grandis are to group, 60 pairs of female male imagos pair of rhizophagus grandis
Group.According to 8 days conventional egg-laying seasons, research and calculate in 8 day egg-laying season, each group egg laying amount change with time situation, every it is female
The eggs on average amount and adult mortality of worm.As a result attached drawing 1-3 is seen respectively.
It can be seen from figure 1 that at first 5 days, accumulative egg laying amount is increased as stocking density increases, but since the 5th day, 40
The accumulative egg laying amount of group is started beyond other each groups.
As it is clear from fig. 2 that in every female adult eggs on average amount, the highest of 40 pairs of groups, 50 pairs and 60 pairs of groups are not so good as 20 pairs instead
With 30 pairs of groups.
It can be seen from figure 3 that adult survival rate is similarly 40 pairs of group highests, and followed by 30 pairs and 50 pairs of groups, 20 pairs and 60 pairs
Group is minimum.
By experimental study, present invention discover that suitable stocking density is particularly important to being proliferated in rhizophagus grandis room.In phase
Under same rearing conditions, the adult of different stocking densities shows apparent difference in terms of egg laying amount and survival rate.Raising
Density is too low, and egg-laying time extends, and not only increases cost of labor but also is unfavorable for guaranteeing adult survival rate；Stocking density is excessively high,
Adult mortality can rise with it, and the spawning rate of unit adult is caused to decline, and increase economic cost.So 30-50 it is right/box
Stocking density can reach the cost-effective target of large-scale production.
The raising of 2 larva of embodiment
Room temperature is 19 DEG C, humidity 75%.
It connects embodiment 1 and carries out subsequent larva raising, after egg hatching is at small grub, be put into 4 yellow meal worms in box daily
Pupa replaces fresh feed daily, allow its in circle culture box feeding yellow meal worm to 2 ages.
Then be moved out being placed on the polystyrene of sawdust cuboid box (length × width × height=18cm × 10cm ×
Raising in 4cm).8mm at the top of the height distance box inside edge that sawdust is placed.5 round culture boxes are put into each cuboid box
Larva, replace fresh feed daily.
It is counted since egg hatching, after 20~22 days, larva is aging.
3 larvae pupation of embodiment and emergence
Room temperature is 19 DEG C, humidity 75%.
Embodiment 2 is connected to be pupated and sprouted wings.Pupate it is husky with 150 DEG C disinfection 2 hours, mixed wet, put with aqua sterilisa later
It is used further to pupate after setting 1d.
Emergence box (cuboid box: length × width × height=18cm × 10cm × 4cm；Polystyrene material) describedization on middle berth
Pupa is husky.
Mature larva was put into emergence box after 40-50 days, and the adult of emergence climbs out of sandbox.
Claims (23)
1. a kind of artificial fecundation method of rhizophagus grandis, which is characterized in that in terms of the floor space for container of laying eggs, with 38-64 it is right/
100cm2Stocking density, be positioned in oviposition container to the female male imago of rhizophagus grandis, and add feed, feed is big swallow wax
Other insects or man-made feeds that the natural host of first, Hosts, rhizophagus grandis can be eaten, feed amount used are oviposition container
Interior one day amount of rhizophagus grandis adult makes adult lay eggs in oviposition container；In terms of the floor space for container of laying eggs, work as oviposition
Ovum grain number amount reaches 110-180/100cm in container2Density when, choose adult；The temperature of feeding environment is 18-25 DEG C,
Humidity is 65-90%.
2. the method as described in claim 1, which is characterized in that the stocking density be 42-60 it is right/100cm2。
3. method according to claim 2, which is characterized in that the stocking density be 45-56 it is right/100cm2。
4. method as claimed in claim 3, which is characterized in that the stocking density be 48-53 it is right/100cm2。
5. method according to any of claims 1-4, which is characterized in that the ovum grain number amount reach 120-170/
100cm2When, choose adult.
6. method as claimed in claim 5, which is characterized in that the ovum grain number amount reaches 130-150/100cm2When, choose
Adult.
7. method according to any of claims 1-4, which is characterized in that the feed is red turpentine beetle, dragon spruce size
Moth, Cabot's Tragopan, calliphorid, sitophilus zea-mais or Callosobruchus chinensis adult, larva, pupa and/or ovum.
8. method according to any of claims 1-4, which is characterized in that after egg hatching is at larva in oviposition container, allow it
In oviposition container then feeding is moved out being placed into larva raising container and continues to feed to 2 ages, feed in container of laying eggs
Dosage be box in one day amount of larva.
9. method according to claim 8, which is characterized in that stocking density of 2 instar larvaes in larva raising container be, with
Larva raising container bottom area meter, 200-350 larva/100cm2。
10. method as claimed in claim 9, which is characterized in that stocking density of 2 instar larvaes in larva raising container be,
In terms of larva raising container bottom area, 250-300 larva/100cm2。
11. method according to claim 8, which is characterized in that after larva is aging, acquisition mature larva, which is put into, is covered with sand of pupating
Emergence container in.
12. method as claimed in claim 11, which is characterized in that described pupate husky is sea sand.
13. method as claimed in claim 11, which is characterized in that it is described pupate it is husky with 150 DEG C disinfection 2 hours, later with going out
Bacterium water mix it is wet, place 1d after be used further to pupate.
14. method according to any of claims 1-4, which is characterized in that the temperature of feeding environment is 19-23 DEG C, humidity
For 70-75%.
15. method as claimed in claim 14, which is characterized in that the temperature of feeding environment is 19-20 DEG C, humidity 70-
75%.
16. method according to any of claims 1-4, which is characterized in that use diameter × height=10cm × 1cm oviposition
Box by 30-50 to the female male imago of rhizophagus grandis to introducing oviposition box, and adds feed, and feed dosage is adult one day in box
Amount is daily 1-4 natural host or 2-3 Hosts, makes its oviposition, when in box of laying eggs ovum grain number amount for 90~
At 140, choose adult.
17. the method described in claim 16, which is characterized in that 35-45 lays eggs to the female male imago of rhizophagus grandis to introducing
Box.
18. method as claimed in claim 17, which is characterized in that by 40 pairs of female male imagos of rhizophagus grandis to introducing oviposition box.
19. the method described in claim 16, which is characterized in that feed dosage is one day amount of adult in box, is every
Its 2 Cabot's Tragopan pupas or 1 red turpentine beetle larva.
20. the method described in claim 16, which is characterized in that when ovum grain number amount is 100~130 in box of laying eggs, choose
Adult out.
21. the method described in claim 16, which is characterized in that after egg hatching is larva in oviposition box, allow it in oviposition box
Then interior feeding is moved out being placed into larva raising box and continues to feed to 2 ages, feed is the daily 2-8 previous day in box of laying eggs
Right host or 3-5 Hosts, up to larva to 2 ages.
22. method as claimed in claim 21, which is characterized in that feed is daily 4 Cabot's Tragopan pupas in oviposition box.
23. the method as described in claim 21 or 22, it is characterised in that use length × width × height=18cm × 10cm × 4cm
Larva raising box, 1 larva raising box put the larva of 4~6 oviposition boxes.
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