CN105754988A - 用于磁珠捕获dna的纯化方法、装置及自动化移液站 - Google Patents
用于磁珠捕获dna的纯化方法、装置及自动化移液站 Download PDFInfo
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- CN105754988A CN105754988A CN201410776600.7A CN201410776600A CN105754988A CN 105754988 A CN105754988 A CN 105754988A CN 201410776600 A CN201410776600 A CN 201410776600A CN 105754988 A CN105754988 A CN 105754988A
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- 238000000034 method Methods 0.000 title abstract description 16
- 239000002480 mineral oil Substances 0.000 claims abstract description 21
- 235000010446 mineral oil Nutrition 0.000 claims abstract description 21
- 238000000746 purification Methods 0.000 claims description 81
- 239000006166 lysate Substances 0.000 claims description 71
- 239000012530 fluid Substances 0.000 claims description 22
- 238000000926 separation method Methods 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 238000011084 recovery Methods 0.000 abstract description 4
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- 238000013467 fragmentation Methods 0.000 description 2
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- YTRQFSDWAXHJCC-UHFFFAOYSA-N chloroform;phenol Chemical compound ClC(Cl)Cl.OC1=CC=CC=C1 YTRQFSDWAXHJCC-UHFFFAOYSA-N 0.000 description 1
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Description
Claims (10)
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201410776600.7A CN105754988B (zh) | 2014-12-15 | 2014-12-15 | 用于磁珠捕获dna的纯化方法、装置及自动化移液站 |
HK16109818.3A HK1221737A1 (zh) | 2014-12-15 | 2016-08-16 | 用於磁珠捕獲 的純化方法、裝置及自動化移液站 |
Applications Claiming Priority (1)
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CN201410776600.7A CN105754988B (zh) | 2014-12-15 | 2014-12-15 | 用于磁珠捕获dna的纯化方法、装置及自动化移液站 |
Publications (2)
Publication Number | Publication Date |
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CN105754988A true CN105754988A (zh) | 2016-07-13 |
CN105754988B CN105754988B (zh) | 2019-05-03 |
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CN201410776600.7A Active CN105754988B (zh) | 2014-12-15 | 2014-12-15 | 用于磁珠捕获dna的纯化方法、装置及自动化移液站 |
Country Status (2)
Country | Link |
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CN (1) | CN105754988B (zh) |
HK (1) | HK1221737A1 (zh) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106546674A (zh) * | 2016-10-20 | 2017-03-29 | 北京蛋白质组研究中心 | 用少量样品快速富集、分离、鉴定和定量内源转录因子及其复合物的方法及其专用装置 |
CN110804608A (zh) * | 2018-08-06 | 2020-02-18 | 绍兴迅敏康生物科技有限公司 | 一种用于磁棒进行磁珠吸附的试剂以及方法 |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101538612A (zh) * | 2009-04-16 | 2009-09-23 | 上海科华生物工程股份有限公司 | 一体化血液核酸筛查平台 |
US20120252115A1 (en) * | 2011-03-29 | 2012-10-04 | Chris Suh | Methods and devices for nucleic acid purification |
CN103269787A (zh) * | 2010-12-21 | 2013-08-28 | 株式会社岛津制作所 | 用于在管内操作对象成分的器件和方法 |
-
2014
- 2014-12-15 CN CN201410776600.7A patent/CN105754988B/zh active Active
-
2016
- 2016-08-16 HK HK16109818.3A patent/HK1221737A1/zh unknown
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101538612A (zh) * | 2009-04-16 | 2009-09-23 | 上海科华生物工程股份有限公司 | 一体化血液核酸筛查平台 |
CN103269787A (zh) * | 2010-12-21 | 2013-08-28 | 株式会社岛津制作所 | 用于在管内操作对象成分的器件和方法 |
US20120252115A1 (en) * | 2011-03-29 | 2012-10-04 | Chris Suh | Methods and devices for nucleic acid purification |
Non-Patent Citations (2)
Title |
---|
JINGYI LI: "《Label-free DNA quantification via a "pipette, aggregate and blot" (PAB) approach with magnetic silica particles on filter paper》", 《THE ROYAL SOCIETY OF CHEMISTRY》 * |
KIMIMICHI OBATA等: "《Development of a novel method for operating magnetic particles, magtration technology, and its use for automating nucleic acid purification 》", 《JOURNAL OF BIOSCIENCE AND BIOENGINEERING》 * |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106546674A (zh) * | 2016-10-20 | 2017-03-29 | 北京蛋白质组研究中心 | 用少量样品快速富集、分离、鉴定和定量内源转录因子及其复合物的方法及其专用装置 |
CN110804608A (zh) * | 2018-08-06 | 2020-02-18 | 绍兴迅敏康生物科技有限公司 | 一种用于磁棒进行磁珠吸附的试剂以及方法 |
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Publication number | Publication date |
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HK1221737A1 (zh) | 2017-06-09 |
CN105754988B (zh) | 2019-05-03 |
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Address after: 518083 comprehensive building of Beishan industrial zone and 11 Building 2, Yantian District, Guangdong, Shenzhen Patentee after: Shenzhen Huada Zhizao Technology Co.,Ltd. Address before: 518083 comprehensive building of Beishan industrial zone and 11 Building 2, Yantian District, Guangdong, Shenzhen Patentee before: MGI TECH Co.,Ltd. |