CN105749995A - Biological chip hybrid device - Google Patents
Biological chip hybrid device Download PDFInfo
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- CN105749995A CN105749995A CN201610274213.2A CN201610274213A CN105749995A CN 105749995 A CN105749995 A CN 105749995A CN 201610274213 A CN201610274213 A CN 201610274213A CN 105749995 A CN105749995 A CN 105749995A
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- biochip
- box
- hybridizing
- box body
- hybridization
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
- B01L3/50855—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates using modular assemblies of strips or of individual wells
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
- B01L3/50853—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates with covers or lids
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0819—Microarrays; Biochips
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/10—Means to control humidity and/or other gases
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
Abstract
The invention discloses a biological chip hybrid device.The device is characterized by comprising a hybrid wet box and hybrid boxes; a plurality of storage cavities used for containing the hybrid boxes are horizontally formed in the hybrid wet box; each hybrid box comprises a hybrid box body, wherein the hybrid box body comprises a horizontal substrate, the substrate is provided with a plurality of indented observation windows in parallel, the edge of each observation window is provided with a vertically through sample adding hole, the two sides of the substrate are both provided with a downwards bent supporting wall, the supporting walls are perpendicular to the substrate, the inner walls of the supporting walls are provided with sliding grooves indented to the outer walls of the supporting walls, and the width of the sliding grooves is matched with the thickness of a biological chip.The device has the advantages that the hybrid boxes are simple in structure, the biological chip is stably supported through the supporting walls, the chip sliding phenomenon is effectively avoided, and the success rate of hybridization is guaranteed; besides, a constant hybrid space is provided for hybridization of the biological chip through the hybrid wet box, other extra auxiliary devices are not needed, and cost is reduced.
Description
Technical field
The present invention relates to biochip technology field, especially relate to a kind of biochip hybridizing device.
Background technology
Along with the fast development of biotechnology and related discipline, the application of biochip is more and more extensive.The application of biochip is it to be hybridized with the biological specimen of labelling, by detecting the quantity of the strong and weak judgement sample target of hybridization signal.
Chinese invention patent 200610114412.3 discloses a kind of biochip hybridizing box, including base, upper cover, clip slot and bracket, this hybridizing box is in use, first biochip is seated on bracket, then substrate is placed on biochip, biochip and substrate are to separate, when using micropipettor that sample is joined conversion zone, less owing to injecting aperture, the suction nozzle of conventional pipettor is easy to drive substrate to be shifted over, thus substrate is taken away biochip surface, namely the phenomenon producing slide plate it is prone to thus causing hybridizing unsuccessfully, generation in order to avoid slide plate phenomenon, some operator can use the micropipettor of minimum suction nozzle, reagent is repeatedly injected, this can cause that bubble enters conversion zone, thus increasing the probability of failure of hybridization；Additionally, after biochip and substrate installation, need to place it in the water of environment such as constant temperature of temperature constant, calorstat or hybrid heater, now need to cover upper cover, by clip slot, upper cover and base sealing are fixed, then whole hybridizing box is placed in water or other corresponding equipment of constant temperature, and above-mentioned sealing fixed form structure is complicated, and clip slot can produce certain extruding force when sealing fixing, sample in chip conversion zone and reagent are likely subject to the effect of this extruding force and are extruded to outside conversion zone, thus affecting the reaction of biochip；The space that the hybridizing box of said structure takies simultaneously is relatively big, and production cost is higher；Additionally constant temperature is it is generally required to complete by extra instrument, and this makes experimental cost higher.
Summary of the invention
The biochip hybridizing device that the technical problem to be solved is to provide a kind of simple in construction, easy for operation, cost is low and portable, this biochip hybridizing device can be prevented effectively from slide plate phenomenon, also can once carry out multi-disc to hybridize simultaneously, can also pass through self to provide the hybridization environment of constant temperature and constant humidity, be hybridized to power height.
This invention address that the technical scheme that above-mentioned technical problem adopts is:
A kind of biochip hybridizing device, including hybridizing wet box and hybridizing box, it is horizontally disposed with multiple storing chamber for putting described hybridizing box in the wet box of described hybridization, described hybridizing box includes hybridizing box box body, described hybridizing box box body includes a horizontally disposed substrate, described substrate is arranged side by side the watch window of multiple concave downward, the edge of described watch window is provided with the well of a up/down perforation, the both sides of described substrate are respectively arranged with the supporting walls of bending downwards, described supporting walls and described substrate are arranged in a mutually vertical manner, the inwall of described supporting walls arranges the chute of oriented described supporting walls outer wall direction depression, the width of described chute and the thickness of biochip match.
The bottom face of described watch window adopts transparent material, when described biochip is inserted in described chute, the sample area of the biochip surface that described watch window alignment is described, being provided with gap between bottom face and the upper surface of described biochip of described watch window, described gap forms chip conversion zone.Watch window bottom face adopts transparent material, when biochip inserts, sample area on biochip can be accurately placed in the lower section of this watch window through this transparent watch window by operator, it is easy to the solution containing hybridization composition and accurately enters into chip conversion zone by well, contact with the sample in biochip surface, complete hybridization, it is ensured that the success rate of hybridization.
Described gap is 0.2-0.5mm.This interstice coverage is suitable chip conversion zone scope.
One end of described substrate is provided with arc-shaped recess.Fetching of biochip is easy in the setting of this arc-shaped recess.
The wet box of described hybridization includes box body, is provided with cavity in described box body, and a sidepiece of described box body is provided with the opening being connected with described cavity, and described opening is provided with lid for closing described cavity movably；
Biochip storing case it is provided with in described cavity, the top of described biochip storing case is provided with the horizontal slot that multiple with described hybridizing box matches, described horizontal slot is described storing chamber, and the insert port of described horizontal slot is arranged on described opening part；
The bottom of described biochip storing case is provided with the long slot of level, and described level long slot insert port is arranged on described opening part, can be provided with humidification disk in the long slot of described level pull；
Being provided with hot plate and heater circuit in described box body, described hot plate electrically connects with described heater circuit, and the outside of described box body is provided with USB charging plug, and described USB charging plug electrically connects with described heater circuit.
Box body and the lid of the wet box of described hybridization all adopt heat-barrier material, it is possible to play good insulation effect.
Above-mentioned hybridization is wet box simple in construction, arranges opening at box body sidepiece, it is simple to picking and placeing of hybridizing box and humidification disk, lid is used for closing cavity, provides a constant environment to the hybridization of biochip；Biochip storing case is set in the cavity of box body, the top of this biochip storing case arranges multiple horizontal slot matched with hybridizing box, hybridizing box can be arranged in horizontal slot pull, simple in construction, it is easy to pick and place, this horizontal slot gives hybridizing box one stable accommodation space simultaneously, can effectively prevent hybridizing box from shaking in crossover process, thus being effectively ensured the success rate of hybridization；The bottom of biochip storing case is provided with the long slot of level, can be provided with humidification disk in the long slot of this level pull, provides the hybridization environment of a constant humidity can to the hybridization of biochip by this humidification disk；Hot plate and heater circuit it is provided with in box body, thering is provided the hybridization environment of a constant temperature by this hot plate and relevant heater circuit to the hybridization of biochip, box portion outside arranges USB charging plug, is charged to heater circuit by this USB charging plug, simple in construction, easy to operate.
The upper surface of the insert port of described horizontal slot and the long slot of described level is provided with the arc convex portion raised up, the lower surface of the insert port of described horizontal slot and the long slot of described level is provided with the arc-shaped recess portion of concave downward, and described arc convex portion is corresponding up and down with the position in described arc-shaped recess portion.The structure of arc convex portion and arc-shaped recess portion phase structure is easy to fetching of hybridizing box and humidification disk.
The bottom of described lid is arranged on the bottom of described opening by gemel connection, the upper interior end face of described lid is provided with hasp, described upper opening portion is provided with the hasp locating slot matched with described hasp, when lid lid holds together, described hasp buckles in described hasp locating slot, and the joint of described lid and described opening is provided with sealing ring.The connection of above-mentioned lid and manner simple in construction, easy to operate, the joint at lid Yu opening arranges sealing ring, it is ensured that when lid is closed, tray interior is sealing state, it is ensured that the sealing of hybridization environment.
Ventilative gap it is provided with, the middle section concave downward of described humidification box between top and the bottom of described biochip storing case.The dampness that humidification disk produces can be supplied to hybridizing box preferably by this ventilative gap, to meet hybridization conditions preferably；The middle section concave downward of humidification disk, this depression can be used for containing water or moistening absorbent paper.
nullThe wet box of described hybridization includes box body and the lid being arranged on described box body，Described box body includes chassis and the accommodation chamber being arranged on described chassis，The described intracavity that holds laterally is set side by side with equivalently-sized front support seat and rear support seat，Described front support seat and described rear support seat interval are arranged，The rear end face of described front support seat、Enclose between front end face and the described both sides inwall holding cavity of described rear support seat and be set as intermediate cavity，It is provided with humidification mechanism in described intermediate cavity，The upper of described front support seat is vertically arranged at intervals with multiple front spacing block，The uniform upper surface being arranged on described front support seat of described front spacing block，The upper of described rear support seat is vertically arranged at intervals with multiple rear spacing block，The uniform upper surface being arranged on described rear support seat of described rear spacing block，Enclose between two described rear spacing blocks that adjacent two described front spacing blocks are adjacent with on vertically correspondence position and be set as described storing chamber，Hot plate and heater circuit it is provided with in described chassis，Described hot plate electrically connects with described heater circuit，The outside of described box body is provided with USB charging plug，Described USB charging plug electrically connects with described heater circuit.The simple in construction of the wet box of above-mentioned hybridization, is set as storing chamber by enclosing between spacing block after two adjacent with on vertically correspondence position of adjacent two front spacing blocks, is placed on it by hybridizing box, provides a stable accommodation space to hybridizing box；It is provided with humidification mechanism in intermediate cavity, the hybridization environment of a constant humidity is provided to the hybridization of biochip by this humidification mechanism；Hot plate and heater circuit it is provided with in chassis, thering is provided the hybridization environment of a constant temperature by this hot plate and relevant heater circuit to the hybridization of biochip, box portion outside arranges USB charging plug, is charged to heater circuit by this USB charging plug, simple in construction, easy to operate.
Described humidification mechanism is water-absorbing sponge.Absorb enough water by water-absorbing sponge, and place it in intermediate cavity, it is possible to ensure to keep the humidity of relative constancy in the wet box heating process of hybridization, to meet hybridization conditions.
The width of the horizontal width between adjacent two described front spacing blocks, adjacent two described rear spacing blocks and described hybridizing box matches, and the vertical width of described intermediate cavity is less than the length of described hybridizing box.Said structure ensures that hybridizing box can stably be put thereon.
The joint of described lid and described box body is provided with sealing ring.Joint at lid Yu box body arranges sealing ring, it is ensured that when lid is closed, tray interior is sealing state, it is ensured that the sealing of hybridization environment.
Compared with prior art, it is an advantage of the current invention that: the hybridizing box simple in construction in this hybrid device, by arranging the supporting walls bending downwards and being vertically arranged in the both sides of substrate, the inwall of supporting walls is provided with the chute matched with biochip thickness, during use, biochip is inserted by this chute the lower section being arranged on substrate, a stable support is provided to biochip by this supporting walls, it is prevented effectively from the generation of slide plate phenomenon, ensure the success rate of hybridization, and provide a constant hybridization space by hybridizing wet box to the hybridization of biochip, without the auxiliary equipment that other are extra, provide cost savings；Substrate is additionally provided with the watch window of multiple concave downward, it is achieved that once carry out multi-disc and hybridize simultaneously.
Accompanying drawing explanation
Fig. 1 is the structural representation of hybridizing box in the embodiment of the present invention one；
Fig. 2 is the structural representation that in the embodiment of the present invention one, biochip is inserted in hybridizing box；
Fig. 3 is the structural representation of the wet box of hybridization in the embodiment of the present invention one；
Fig. 4 is the sectional structure schematic diagram of the wet box of hybridization in the embodiment of the present invention one；
Fig. 5 is the structural representation of humidification box in the embodiment of the present invention one；
Fig. 6 is the structural representation of the wet box of hybridization in the embodiment of the present invention two；
Fig. 7 is the sectional structure schematic diagram of the wet box box body of hybridization in the embodiment of the present invention two；
Fig. 8 is the structural representation of hybridizing box in the embodiment of the present invention three；
Fig. 9 is the structural representation that in the embodiment of the present invention three, biochip is inserted in hybridizing box.
Detailed description of the invention
Below in conjunction with accompanying drawing embodiment, the present invention is described in further detail.
Embodiment one: such as Fig. 1, shown in Fig. 2, a kind of biochip hybridizing device, including hybridizing wet box A and hybridizing box B, it is horizontally disposed with multiple storing chamber for putting hybridizing box B in the wet box A of hybridization, hybridizing box B includes hybridizing box body, hybridizing box body includes a horizontally disposed substrate 1, substrate 1 is arranged side by side the watch window 11 of multiple concave downward, the edge of watch window 11 is provided with the well 12 of a up/down perforation, the both sides of substrate 1 are respectively arranged with the supporting walls 2 of bending downwards, supporting walls 2 and substrate 1 are arranged in a mutually vertical manner, the inwall of supporting walls 2 arranges the chute 21 of oriented supporting walls 2 outer wall direction depression, the thickness of the width of chute 21 and biochip 3 matches.
In this particular embodiment, the bottom face of watch window 11 adopts transparent material, when biochip 3 is inserted in chute 21, watch window 11 is directed at the sample area on biochip 3 surface, being provided with gap between bottom face and the upper surface of biochip 3 of watch window 11, this gap forms chip conversion zone.Watch window 11 bottom face adopts transparent material, when biochip 3 inserts, sample area on biochip 3 can be accurately placed in the lower section of this watch window 11 through this transparent watch window 11 by operator, it is easy to the solution containing hybridization composition and accurately enters into chip conversion zone by well 12, contact with the sample on biochip 3 surface, complete hybridization, it is ensured that the success rate of hybridization.
In this particular embodiment, above-mentioned gap is 0.2mm.
In this particular embodiment, well 12 aperture from top to bottom is gradually little of funnel-form, suitable with suction pipette head, it is simple to the insertion of suction pipette head, makes solution can accurately drip to the sample region location on biochip 3 surface simultaneously.
In this particular embodiment, as shown in Figures 3 to 5, the wet box A of hybridization includes box body 4, is provided with cavity in box body 4, and a sidepiece of box body 4 is provided with the opening 41 being connected with cavity, and opening 41 is provided with lid 42 for closing cavity movably；
In this particular embodiment, biochip storing case 5 it is provided with in cavity, the top of biochip storing case 5 is provided with multiple with the hybridizing box B horizontal slot 51 matched, and horizontal slot 51 is the storing chamber for putting hybridizing box B, and the insert port of horizontal slot 51 is arranged on opening 41 place；
In this particular embodiment, the bottom of biochip storing case 5 is provided with the long slot 52 of level, and the insert port of the long slot 52 of level is arranged on opening 41 place, can be provided with humidification disk 6 in the long slot 52 of level pull；
In this particular embodiment, being provided with hot plate 7 and heater circuit (not shown) in box body 4, hot plate 7 electrically connects with heater circuit, and the outside of box body 4 is provided with USB charging plug 8, USB charging plug 8 and electrically connects with heater circuit.
In this particular embodiment, box body 4 and the lid 42 of hybridizing wet box A all adopt heat-barrier material, it is possible to play good insulation effect.
In this particular embodiment, heater circuit is provided with Temperature Controlling Chip, controls the temperature in the wet box A of hybridization by this Temperature Controlling Chip, to provide an isoperibol accurately.
In this particular embodiment, the levels of box body 4 is provided with hot plate 7, improves the efficiency of heating surface so that the temperature of levels keeps consistent.
Above-mentioned hybridization is wet box A simple in construction, arranges opening 41 at box body 4 sidepiece, it is simple to picking and placeing of hybridizing box B and humidification disk 6, lid 42 is used for closing cavity, provides a constant environment to the hybridization of biochip 3；Biochip storing case 5 is set in the cavity of box body 4, the top of this biochip storing case 5 arranges multiple with the hybridizing box B horizontal slot 51 matched, hybridizing box 51 can be arranged in horizontal slot 51 pull, simple in construction, it is easy to pick and place, this horizontal slot 51 gives mono-stable accommodation space of hybridizing box B simultaneously, can effectively prevent hybridizing box B from shaking in crossover process, thus being effectively ensured the success rate of hybridization；The bottom of biochip storing case 5 is provided with the long slot 52 of level, can be provided with humidification disk 6 in the long slot 52 of this level pull, provides the hybridization environment of a constant humidity can to the hybridization of biochip 3 by this humidification disk 6；Hot plate 7 and heater circuit it is provided with in box body 4, thering is provided the hybridization environment of a constant temperature by this hot plate 7 and relevant heater circuit to the hybridization of biochip 3, box portion outside arranges USB charging plug 8, is charged to heater circuit by this USB charging plug 8, simple in construction, easy to operate.
In this particular embodiment, the upper surface of the insert port of horizontal slot 51 and the long slot 52 of level is provided with the arc convex portion c raised up, it is corresponding up and down with the position of arc-shaped recess portion d that the lower surface of the insert port of horizontal slot 51 and the long slot 52 of level is provided with the arc-shaped recess portion d of concave downward, arc convex portion c.The structure of arc convex portion c and arc-shaped recess portion d phase structure is easy to fetching of hybridizing box B and humidification disk 6.
In this particular embodiment, the bottom of lid 42 connects the bottom being arranged on opening 41 by hinge (not shown), the upper interior end face of lid 42 is provided with hasp 421, opening 41 top is provided with the hasp locating slot 411 matched with hasp 421, when lid 42 lid holds together, hasp 421 buckles in hasp locating slot 411, and the joint of lid 42 and opening 41 is provided with sealing ring e.The connection of above-mentioned lid 42 and manner simple in construction, easy to operate, the joint at lid 42 with opening 41 arranges sealing ring e, it is ensured that when lid 42 is closed, and box body 4 is internal is sealing state, it is ensured that the sealing of hybridization environment.
In this particular embodiment, between top and the bottom of biochip storing case 5, it is provided with ventilative gap, middle section 61 concave downward of humidification box 6.The dampness that humidification disk 6 produces can be supplied to hybridizing box preferably by this ventilative gap, to meet hybridization conditions preferably；Middle section 61 concave downward of humidification disk 6, this depression can be used for containing water or moistening absorbent paper.
In this particular embodiment, the top of biochip storing case 5 is divided into multiple rows of structure from top to bottom, every row of horizontal is set side by side with multiple horizontal slot 51, the long slot 52 of level is arranged under bottom horizontal slot 51, it is provided with ventilative gap between each horizontal slot 51, the dampness that humidification disk produces can be supplied to hybridizing box preferably by this ventilative gap, to meet hybridization conditions preferably.
Embodiment two: such as Fig. 1, shown in Fig. 2, a kind of biochip hybridizing device, including hybridizing wet box A and hybridizing box B, it is horizontally disposed with multiple storing chamber for putting hybridizing box B in the wet box A of hybridization, hybridizing box B includes hybridizing box body, hybridizing box body includes a horizontally disposed substrate 1, substrate 1 is arranged side by side the watch window 11 of multiple concave downward, the edge of watch window 11 is provided with the well 12 of a up/down perforation, the both sides of substrate 1 are respectively arranged with the supporting walls 2 of bending downwards, supporting walls 2 and substrate 1 are arranged in a mutually vertical manner, the inwall of supporting walls 2 arranges the chute 21 of oriented supporting walls 2 outer wall direction depression, the thickness of the width of chute 21 and biochip 3 matches.
In this particular embodiment, the bottom face of watch window 11 adopts transparent material, when biochip 3 is inserted in chute 21, watch window 11 is directed at the sample area on biochip 3 surface, being provided with gap between bottom face and the upper surface of biochip 3 of watch window 11, this gap forms chip conversion zone.Watch window 11 bottom face adopts transparent material, when biochip 3 inserts, sample area on biochip 3 can be accurately placed in the lower section of this watch window 11 through this transparent watch window 11 by operator, it is easy to the solution containing hybridization composition and accurately enters into chip conversion zone by well 12, contact with the sample on biochip 3 surface, complete hybridization, it is ensured that the success rate of hybridization.
In this particular embodiment, above-mentioned gap is 0.2mm.
In this particular embodiment, well 12 aperture from top to bottom is gradually little of funnel-form, suitable with suction pipette head, it is simple to the insertion of suction pipette head, makes solution can accurately drip to the sample region location on biochip 3 surface simultaneously.
nullIn this particular embodiment，Such as Fig. 6、Shown in Fig. 7，The wet box A of hybridization includes box body A4 and the lid A5 being arranged on box body A4，Box body A4 includes chassis A41 and the accommodation chamber A42 being arranged on the A41 of chassis，Hold intracavity and be laterally set side by side with equivalently-sized front support seat A6 and rear support seat A7，Front support seat A6 and rear support seat A7 interval are arranged，The rear end face of front support seat A6、Enclose between front end face and the both sides inwall holding chamber A42 of rear support seat A7 and be set as intermediate cavity A421，Humidification mechanism it is provided with in intermediate cavity A421，The upper of front support seat A6 is vertically arranged at intervals with multiple front spacing block A61，The uniform upper surface being arranged on front support seat A6 of front spacing block A61，The upper of rear support seat A7 is vertically arranged at intervals with multiple rear spacing block A71，The uniform upper surface being arranged on rear support seat A7 of rear spacing block A71，Enclose, between spacing block A71, the storing chamber being set as putting hybridizing box B after adjacent with on vertically correspondence position for adjacent two front spacing block A61 two，Hot plate A8 and heater circuit (not shown) it is provided with in the A41 of chassis，Hot plate A8 electrically connects with heater circuit，The outside of box body A4 is provided with USB charging plug A9，USB charging plug A9 electrically connects with heater circuit.The simple in construction of the wet box A of above-mentioned hybridization, is set as storing chamber by enclosing between spacing block A71 after adjacent with on vertically correspondence position for adjacent two front spacing block A61 two, is placed on it by hybridizing box B, provides a stable accommodation space to hybridizing box B；It is provided with humidification mechanism in intermediate cavity A421, the hybridization environment of a constant humidity is provided to the hybridization of biochip 3 by this humidification mechanism；Hot plate A8 and heater circuit it is provided with in the A41 of chassis, the hybridization environment of a constant temperature is provided to the hybridization of biochip 3 by this hot plate A8 and relevant heater circuit, USB charging plug A9 is set outside box body A4, charged to heater circuit by this USB charging plug A9, simple in construction, easy to operate.
In this particular embodiment, humidification mechanism is water-absorbing sponge (not shown).Absorb enough water by water-absorbing sponge, and place it in intermediate cavity A421, it is possible to ensure to keep in heating process at the wet box A of hybridization the humidity of relative constancy, to meet hybridization conditions.
In this particular embodiment, adjacent two front spacing block A61, horizontal width between spacing block A71 matches with the width of hybridizing box B after adjacent two, the vertical width of the intermediate cavity A421 length less than hybridizing box B.Said structure ensures that hybridizing box B can stably put thereon.
In this particular embodiment, the joint of lid A5 and box body A4 is provided with sealing ring (not shown).Joint at lid A5 Yu box body A4 arranges sealing ring, it is ensured that when lid A5 closes, and box body A4 is internal is sealing state, it is ensured that the sealing of hybridization environment.
In this particular embodiment, heater circuit is provided with Temperature Controlling Chip, controls the temperature in the wet box A of hybridization by this Temperature Controlling Chip, to provide an isoperibol accurately.
Embodiment three: other parts and embodiment one, embodiment two are identical, and it is different in that one end of substrate 1 is provided with arc-shaped recess 13.Fetching of biochip 3 is easy in the setting of this arc-shaped recess 13, as shown in Figure 8, Figure 9.
Embodiment four: other parts and embodiment one, embodiment two are identical, and it is different in that gap is 0.3mm.
Embodiment five: other parts and embodiment one, embodiment two are identical, and it is different in that gap is 0.4mm.
Embodiment six: other parts and embodiment one, embodiment two are identical, and it is different in that gap is 0.5mm.
Claims (10)
1. a biochip hybridizing device, it is characterized in that including hybridizing wet box and hybridizing box, it is horizontally disposed with multiple storing chamber for putting described hybridizing box in the wet box of described hybridization, described hybridizing box includes hybridizing box box body, described hybridizing box box body includes a horizontally disposed substrate, described substrate is arranged side by side the watch window of multiple concave downward, the edge of described watch window is provided with the well of a up/down perforation, the both sides of described substrate are respectively arranged with the supporting walls of bending downwards, described supporting walls and described substrate are arranged in a mutually vertical manner, the inwall of described supporting walls arranges the chute of oriented described supporting walls outer wall direction depression, the width of described chute and the thickness of biochip match.
2. a kind of biochip hybridizing device as claimed in claim 1, it is characterized in that the bottom face of described watch window adopts transparent material, when described biochip is inserted in described chute, the sample area of the biochip surface that described watch window alignment is described, being provided with gap between bottom face and the upper surface of described biochip of described watch window, described gap forms chip conversion zone.
3. a kind of biochip hybridizing device as claimed in claim 1, it is characterised in that one end of described substrate is provided with arc-shaped recess.
4. a kind of biochip hybridizing device as claimed in claim 1, it is characterized in that the wet box of described hybridization includes box body, it is provided with cavity in described box body, one sidepiece of described box body is provided with the opening being connected with described cavity, and described opening is provided with lid for closing described cavity movably；
Biochip storing case it is provided with in described cavity, the top of described biochip storing case is provided with the horizontal slot that multiple with described hybridizing box matches, described horizontal slot is described storing chamber, and the insert port of described horizontal slot is arranged on described opening part；
The bottom of described biochip storing case is provided with the long slot of level, and described level long slot insert port is arranged on described opening part, can be provided with humidification disk in the long slot of described level pull；
Being provided with hot plate and heater circuit in described box body, described hot plate electrically connects with described heater circuit, and the outside of described box body is provided with USB charging plug, and described USB charging plug electrically connects with described heater circuit.
5. a kind of biochip hybridizing device as claimed in claim 4, it is characterized in that the upper surface of the insert port of described horizontal slot and the long slot of described level is provided with the arc convex portion raised up, the lower surface of the insert port of described horizontal slot and the long slot of described level is provided with the arc-shaped recess portion of concave downward, and described arc convex portion is corresponding up and down with the position in described arc-shaped recess portion.
6. a kind of biochip hybridizing device as claimed in claim 4, it is characterized in that the bottom of described lid is arranged on the bottom of described opening by gemel connection, the upper interior end face of described lid is provided with hasp, described upper opening portion is provided with the hasp locating slot matched with described hasp, when lid lid holds together, described hasp buckles in described hasp locating slot, and the joint of described lid and described opening is provided with sealing ring.
7. a kind of biochip hybridizing device as claimed in claim 1, it is characterised in that be provided with ventilative gap between top and the bottom of described biochip storing case, the middle section concave downward of described humidification box.
null8. a kind of biochip hybridizing device as claimed in claim 1，It is characterized in that the wet box of described hybridization includes box body and covers the lid being located on described box body，Described box body includes chassis and the accommodation chamber being arranged on described chassis，The described intracavity that holds laterally is set side by side with equivalently-sized front support seat and rear support seat，Described front support seat and described rear support seat interval are arranged，The rear end face of described front support seat、Enclose between front end face and the described both sides inwall holding cavity of described rear support seat and be set as intermediate cavity，It is provided with humidification mechanism in described intermediate cavity，The upper of described front support seat is vertically arranged at intervals with multiple front spacing block，The uniform upper surface being arranged on described front support seat of described front spacing block，The upper of described rear support seat is vertically arranged at intervals with multiple rear spacing block，The uniform upper surface being arranged on described rear support seat of described rear spacing block，Enclose between two described rear spacing blocks that adjacent two described front spacing blocks are adjacent with on vertically correspondence position and be set as described storing chamber，Hot plate and heater circuit it is provided with in described chassis，Described hot plate electrically connects with described heater circuit，The outside of described box body is provided with USB charging plug，Described USB charging plug electrically connects with described heater circuit.
9. a kind of biochip hybridizing device as claimed in claim 8, it is characterized in that the width of the horizontal width between adjacent two described front spacing blocks, adjacent two described rear spacing blocks and described hybridizing box matches, the vertical width of described intermediate cavity is less than the length of described hybridizing box.
10. a kind of biochip hybridizing device as claimed in claim 8, it is characterised in that the joint of described lid and described box body is provided with sealing ring.
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| CN201610274213.2A CN105749995B (en) | 2016-04-28 | 2016-04-28 | A kind of biochip hybridizing device |
| US15/466,573 US20170191121A1 (en) | 2016-04-28 | 2017-03-22 | Biochip hybridization device |
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| CN201610274213.2A CN105749995B (en) | 2016-04-28 | 2016-04-28 | A kind of biochip hybridizing device |
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| CN105749995A true CN105749995A (en) | 2016-07-13 |
| CN105749995B CN105749995B (en) | 2017-10-31 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107338288A (en) * | 2017-06-14 | 2017-11-10 | 杨华卫 | A kind of biomolecule detecting method |
| CN108693011A (en) * | 2018-05-14 | 2018-10-23 | 柳州市妇幼保健院 | A kind of aobvious tape drums of chromosome N |
| CN113514644A (en) * | 2021-04-28 | 2021-10-19 | 中农康正技术服务有限公司 | Detection chip is selected for use to quick open-close type antibody screening that facilitates use |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2793088Y (en) * | 2005-05-30 | 2006-07-05 | 西安交通大学 | Biological chip hybridization reacting box |
| CN1948479A (en) * | 2006-11-09 | 2007-04-18 | 博奥生物有限公司 | Biochip hybridizing box |
| US20090017505A1 (en) * | 2006-05-22 | 2009-01-15 | Micronas Holding Gmbh | Process and device for mixing microdroplets |
| CN201785381U (en) * | 2010-04-15 | 2011-04-06 | 四川国兴盛生物科技有限公司 | Foldable hybridization box |
| CN204958886U (en) * | 2015-06-10 | 2016-01-13 | 张璐华 | Biochip reaction box |
| CN205133588U (en) * | 2015-11-20 | 2016-04-06 | 北京泱深生物信息技术有限公司 | Genetic chip placer |
| CN205761217U (en) * | 2016-04-28 | 2016-12-07 | 宁波大学 | A kind of biochip hybridizing device |
-
2016
- 2016-04-28 CN CN201610274213.2A patent/CN105749995B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2793088Y (en) * | 2005-05-30 | 2006-07-05 | 西安交通大学 | Biological chip hybridization reacting box |
| US20090017505A1 (en) * | 2006-05-22 | 2009-01-15 | Micronas Holding Gmbh | Process and device for mixing microdroplets |
| CN1948479A (en) * | 2006-11-09 | 2007-04-18 | 博奥生物有限公司 | Biochip hybridizing box |
| CN201785381U (en) * | 2010-04-15 | 2011-04-06 | 四川国兴盛生物科技有限公司 | Foldable hybridization box |
| CN204958886U (en) * | 2015-06-10 | 2016-01-13 | 张璐华 | Biochip reaction box |
| CN205133588U (en) * | 2015-11-20 | 2016-04-06 | 北京泱深生物信息技术有限公司 | Genetic chip placer |
| CN205761217U (en) * | 2016-04-28 | 2016-12-07 | 宁波大学 | A kind of biochip hybridizing device |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107338288A (en) * | 2017-06-14 | 2017-11-10 | 杨华卫 | A kind of biomolecule detecting method |
| CN108693011A (en) * | 2018-05-14 | 2018-10-23 | 柳州市妇幼保健院 | A kind of aobvious tape drums of chromosome N |
| CN113514644A (en) * | 2021-04-28 | 2021-10-19 | 中农康正技术服务有限公司 | Detection chip is selected for use to quick open-close type antibody screening that facilitates use |
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|---|---|
| CN105749995B (en) | 2017-10-31 |
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Effective date of registration: 20190521 Address after: 315040 Ningbo New Materials Innovation Center East District, Ningbo High-tech Zone, Zhejiang Province, No. 1 11-2-1 Patentee after: Zhejiang Zhenghegu Biotechnology Co., Ltd. Address before: 315201 No. 777, Zhong Guan Xi Road, Zhuang City Street, Zhenhai District, Ningbo, Zhejiang. Patentee before: Ningbo Anxin Biological Technology Co., Ltd. |