CN2793088Y - Biological chip hybridization reacting box - Google Patents
Biological chip hybridization reacting box Download PDFInfo
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- CN2793088Y CN2793088Y CN 200520078844 CN200520078844U CN2793088Y CN 2793088 Y CN2793088 Y CN 2793088Y CN 200520078844 CN200520078844 CN 200520078844 CN 200520078844 U CN200520078844 U CN 200520078844U CN 2793088 Y CN2793088 Y CN 2793088Y
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- cover plate
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Abstract
The utility model relates to a biological chip hybridization reaction box, which comprises a loading pressing bracket and a base arranged in the loading pressing bracket, wherein the upper end of the base in the loading pressing bracket is also successively provided with a cover plate and a pressing plate, the base, the cover plate and the pressing plate are fixed into an integral body by a fixing piece arranged on the loading pressing bracket. The utility model causes the chip to form a minute seal cavity under the function of external force through the base and the cover plate, and realizes favorable sealing property. The utility model has the advantages of simple structure, convenient operation, relative stability of liquid capacity and little waste of hybridisation liquid.
Description
Technical field
The utility model relates to a kind of biochemical reaction utility appliance, particularly a kind of biochip hybridization reaction box.
Background technology
Along with deepening constantly of functional genome research, biotechnology and related discipline have obtained further development, biochip technology also from original technological concept step transition to the technology application stage, and become the important detection means in fields such as biology, medicine, agricultural, environmental protection, the administration of justice.The concrete application process of biochip is that chip and fluorescently-labeled biological specimen are hybridized, by detecting the quantity that intensity of hybridization signal is come target molecule in the judgement sample.Along with the development of biochip technology, relevant experiment proofing unit also arises at the historic moment, and biochip hybridizing box is exactly wherein a kind of.The main application of hybridizing box just provides the place of a fluorescent mark target molecule to be measured and biochip hybridization, guarantees that hybridization carries out in set reaction system.The hybridizing box device is varied, has different constructional features, and also each is variant for working method.Still mainly be divided into following several modes at present: 1, cover glass formula hybridizing box at the hybridizing box that uses.This hybridizing box is announced (http://cmgm.stanford.edu/pbrown/protocols/index.html) by Stanford Univ USA Brown laboratory the earliest: it is that the hybridization mixed solution is dropped on the cover glass, then biochip point sample is faced lower cover on cover glass, around cover glass, seal with seal gum, between cover glass and chip, form airtight solution hybridization space, place thermostat container to hybridize chip then.The advantage of this method is hybridization solution consumption seldom (about 10 μ L), easily produces bubble when shortcoming is operation, influences results of hybridization, because cover glass directly contacts with chip point sample zone, is easy to generate cut in addition.Afterwards, on this method basis, produced the hybridizing box (production of ArrayIt company) of single cabin formula again, but just reduced with this step of seal gum sealing cover slide, the chip that will be stamped cover glass that changes is sealed in single cabin formula hybridizing box of the special use that is placed with 1 centimeter square suction paper handkerchief in advance and carries out constant temperature hybridization, but does not improve above-mentioned technological deficiency; 2, chip immersion hybridizing box.The product that has U.S. Clontech company to produce that this hybridizing box is common.It is that biochip is positioned in the hybridizing box, injects then and contains the hybridization solution of target molecule to be measured in box.This method is easy to grasp, but hybridization solution submergence chip (needing 2mL approximately) fully, and this consumption is unpractical for the experimental analysis of some sample preciousness; 3, partition type hybridizing box.This kind hybridizing box is the most representative to be that U.S. Agilent company produces " Legacy 6 screwchamber " series and the new Surehyb that releases
TMSeries hybridizing box product.The hybridizing box of this form is made up of consumptive materials such as two hybridizing box metal frames and pad, packing ring, Pi Sai up and down.At first the chip point sample is faced up, lies on lower floor's carriage of hybridizing box, pad is covered point sample zone on the chip, pad contact with chip one side around rib shape projection is arranged, therefore can above chip point sample district, form the cavity of fixed volume.There are well and venting hole in the pad top.At pad upper fixed rubber cradle, cover the upper strata square frame of hybridizing box then, tighten the screws is fixed.Well from the pad top adds hybridization solution subsequently.Its advantage is that hybridization step is easy to stdn, but that shortcoming is a step is loaded down with trivial details relatively, and proprietary annex is more, and accuracy requirement is higher, and the hybridizing box frame part adopts metal to make in addition, and precision prescribed is also very high, so cost is higher, is not easy to promote the use of; 4, disposable self-styled hybridizing box.This hybridizing box is a medical disposable material, is made up of plastic sheet suitable with the hybridization region domain sizes and viscosity packing ring, is attached to the hybridization zone during use.Have well on plastic sheet, hybridization solution adds thus, with the viscosity small pieces well sealing can be hybridized then.This method also can form the hybridization zone of fixed system as above-mentioned the third hybridizing box, and consumptive material is also simple relatively.But shortcoming is need carefully stick hybridizing box when using at every turn, the certain skill of manipulation require, and also this hybridizing box is easy to leakage, so experimental repeatability is bad, and wayward, usually influence the experiment process.
Summary of the invention
The purpose of this utility model is to overcome the shortcoming of above-mentioned prior art, and a kind of biochip hybridization reaction box simple in structure, easy and simple to handle is provided.
For reaching above purpose, the utility model is by the following technical solutions: comprising that dress is pressed support and is arranged on dress presses pedestal in the support, be characterized in, the upper end of pedestal also is disposed with cover plate and pressing plate in dress is pressed support, and pedestal, cover plate and pressing plate press the mounting block on the support to be fixed as one by being arranged on dress.
Another characteristics of the present utility model are: the lower surface of cover plate is provided with the boss that is used to form cavity, and boss is provided with the groove of sealing, and the upper surface of cover plate is provided with the liquid filling hole that is connected with cavity; Pressing plate is the sphere of movements for the elephants type pressing plate that rigid material is made; Also be provided with steady brace on the pedestal, cover plate is provided with and the steady brace corresponding positioning hole.
The utility model forms tiny annular seal space by the base and cover sheet with chip under the effect of external force, realized good sealing property, and simple in structure, easy to operate, and liquid capacity is relatively stable, and the waste of hybridization liquid is little.
Description of drawings
Fig. 1 is an one-piece construction synoptic diagram of the present utility model;
Fig. 2 is the elevational cross-sectional view of Fig. 1;
Fig. 3 is the front view of the utility model cover plate 4;
Fig. 4 is the left view of the utility model cover plate 4;
Fig. 5 is the vertical view of the utility model cover plate 4.
Embodiment
Below in conjunction with accompanying drawing the utility model is described in further detail.
Referring to Fig. 1,2, the utility model comprises dress pressure support 2, be disposed with the sphere of movements for the elephants type pressing plate 3 that pedestal 6, cover plate 4 and rigid material that plastics make are made in dress is pressed support 2 from bottom to top, pedestal 6, cover plate 4 and pressing plate 3 press the mounting block 1 on the support 2 to be fixed as one by being arranged on dress in use; And be provided with the boss 10 that is used to form cavity 11 in the lower surface of cover plate 4, boss 10 is provided with the groove 12 of sealing, and the upper surface of cover plate 4 is provided with the liquid filling hole 9 that is connected with cavity 11, and liquid filling hole 9 passes the matrix pattern gap of pressing plate 3 in use.
Referring to Fig. 3,4,5, the lower surface of the utility model cover plate 4 is provided with the dual seal chamber 11 that forms by boss 10, for preventing the seepage of liquid, also be provided with the groove 12 of sealing on boss 10, and also be provided with pilot hole 7 on cover plate 4, pedestal 6 is provided with and pilot hole 7 corresponding steady braces 8.
At first biochip 5 is placed on the pedestal 6 in use, the one side that again cover plate 4 is provided with cavity 11 be placed on biochip 5 above, pedestal 6 is provided with steady brace 8, cover plate 4 is provided with and steady brace 8 corresponding positioning hole 7, prevent the slip of cover plate 4 during assembling by the location of steady brace 8 and pilot hole 7, and the probe array on the destruction biochip 5, again field type pressing plate 3 is placed on the cover plate 4, place dress to press in the support 2 then, by field type pressing plate 3 power that applies is delivered to boss 10 and biochip 5 formed annular seal spaces uniformly by mounting block 1, thereby reach good sealing effectiveness, liquid fillers injects hybridization liquid by liquid filling hole 9 in hybridizing box, carry out hybridization then in water bath.
The utility model is primarily aimed at precious biological sample hybridization and design, and simple in structure, easy to operate, liquid capacity is relatively stable, and the waste of hybridization liquid is little, need not special operating skill.
Claims (4)
1, a kind of biochip hybridization reaction box, comprise dress pressure support [2] and be arranged on dress and press the interior pedestal [6] of support [2], it is characterized in that: the upper end of pedestal [6] also is disposed with cover plate [4] and pressing plate [3] in dress is pressed support [2], and pedestal [6], cover plate [4] and pressing plate [3] press the mounting block [1] on the support [2] to be fixed as one by being arranged on dress.
2, biochip hybridization reaction box according to claim 1, it is characterized in that: the lower surface of said cover plate [4] is provided with the boss [10] that is used to form cavity [11], boss [10] is provided with the groove [12] of sealing, and the upper surface of cover plate [4] is provided with the liquid filling hole [9] that is connected with cavity [11].
3, biochip hybridization reaction box according to claim 1 is characterized in that: the sphere of movements for the elephants type pressing plate that said pressing plate [3] is made for rigid material.
4, biochip hybridization reaction box according to claim 1 is characterized in that: also be provided with steady brace [8] on the said pedestal [6], cover plate [4] is provided with and steady brace [8] corresponding positioning hole [7].
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200520078844 CN2793088Y (en) | 2005-05-30 | 2005-05-30 | Biological chip hybridization reacting box |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200520078844 CN2793088Y (en) | 2005-05-30 | 2005-05-30 | Biological chip hybridization reacting box |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2793088Y true CN2793088Y (en) | 2006-07-05 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200520078844 Expired - Fee Related CN2793088Y (en) | 2005-05-30 | 2005-05-30 | Biological chip hybridization reacting box |
Country Status (1)
| Country | Link |
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| CN (1) | CN2793088Y (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103808771A (en) * | 2012-11-15 | 2014-05-21 | 赵朝辉 | Biochemical reaction basin for electrochemical biochip |
| CN104152342A (en) * | 2014-07-09 | 2014-11-19 | 湖北省农业科学院畜牧兽医研究所 | Constant-pressure transmembrane clamp for hybridization of Southern imprinting |
| CN105219633A (en) * | 2014-05-27 | 2016-01-06 | 昆明寰基生物芯片产业有限公司 | Microarray hybridization module |
| CN105296465A (en) * | 2014-06-13 | 2016-02-03 | 康隽生物科技股份有限公司 | Biological reaction method of biological molecule reaction device |
| CN105749995A (en) * | 2016-04-28 | 2016-07-13 | 宁波大学 | Biological chip hybrid device |
-
2005
- 2005-05-30 CN CN 200520078844 patent/CN2793088Y/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103808771A (en) * | 2012-11-15 | 2014-05-21 | 赵朝辉 | Biochemical reaction basin for electrochemical biochip |
| CN105219633A (en) * | 2014-05-27 | 2016-01-06 | 昆明寰基生物芯片产业有限公司 | Microarray hybridization module |
| CN105296465A (en) * | 2014-06-13 | 2016-02-03 | 康隽生物科技股份有限公司 | Biological reaction method of biological molecule reaction device |
| CN104152342A (en) * | 2014-07-09 | 2014-11-19 | 湖北省农业科学院畜牧兽医研究所 | Constant-pressure transmembrane clamp for hybridization of Southern imprinting |
| CN104152342B (en) * | 2014-07-09 | 2016-08-17 | 湖北省农业科学院畜牧兽医研究所 | Southern marking hybridization constant voltage transferring film is pressed from both sides |
| CN105749995A (en) * | 2016-04-28 | 2016-07-13 | 宁波大学 | Biological chip hybrid device |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |