CN105296465A - Biological reaction method of biological molecule reaction device - Google Patents
Biological reaction method of biological molecule reaction device Download PDFInfo
- Publication number
- CN105296465A CN105296465A CN201410261281.6A CN201410261281A CN105296465A CN 105296465 A CN105296465 A CN 105296465A CN 201410261281 A CN201410261281 A CN 201410261281A CN 105296465 A CN105296465 A CN 105296465A
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- China
- Prior art keywords
- biological respinse
- biological
- reaction
- biomolecular
- microscope carrier
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Abstract
A biological reaction method of a biological molecule reaction device comprises the following steps of providing biological molecules loaded on a biological molecule reaction carrying table; placing the biological molecules in a reaction solution; and placing the reaction solution in a reaction tank in a biological reaction box to perform biological reaction. The biological molecule reaction carrying table can be maintained at a fixed position in the reaction tank of the biological reaction box by a carrying table fixing device, so that the biological molecule reaction carrying table cannot move when mixing and shaking are carried out in an operation experiment; moreover, the biological reaction box is combined with a sealing box upper cover with a sealing ring in an airtight mode so as to seal the reaction tank, and the reaction solution in the reaction tank is prevented from overflowing and evaporating. Therefore, the biological molecule reaction device assists in operation of a biological molecule experiment, all biological experiment procedures can be finished in the device, the biological molecule reaction carrying table can be protected, other consumable items are reduced, efficiency of operation of the biological molecule experiment is improved effectively, and space of other equipment can be saved.
Description
Technical field
The present invention has a kind of method of carrying out biological respinse about biomolecular reaction device; espespecially relate to a kind of can biomolecular reaction device assist operation biomolecular experiments method; refer in particular to and can in the apparatus all biological experiment flow be completed; protection biomolecular reaction microscope carrier can be reached and reduce other and use consumptive material; effective efficiency increasing operation biomolecular experiments, and save the method in miscellaneous equipment space.
Background technology
For adapting to the operation experiments of various biomolecules microscope carrier in recent years, in operating process, biomolecular reaction must be had to load biological microscope carrier, and it has certain space and reaction reagent is housed, and makes reagent and microscope carrier do abundant reaction.And general used biomolecular reaction, all there is no proprietary formats, arbitrarily with commercially available plastics bag, plastics casing or plastic medicine box as reactive tank, those plastic containers are not only not of uniform size, and form and biomolecules microscope carrier are not also harmonious, biological respinse liquid is caused easily to overflow in the process of operation.Therefore, tradition is when carrying out biomolecular reaction, due to plastic containers without normalization, inconvenience and be not suitable for the shortcomings such as microscope carrier, cause biomolecules microscope carrier place inconvenience, fixing, easily slide, and then cause inconvenience when operating biomolecular reaction experiment and error.Therefore, generally cannot meet user and operation biomolecular experiments can be made when reality uses to reach the requirement of stability and simplicity.
Summary of the invention
Main purpose of the present invention is, overcomes the problems referred to above that known technology meets with and provides a kind of biomolecular reaction device making operation biomolecular experiments reach stability and simplicity to carry out the method for biological respinse.
Secondary objective of the present invention is, provides one can operate biomolecular experiments in order to assistance, by the method that all biological experiment flow completes in biomolecular reaction device.
Another object of the present invention is to; there is provided one can reach protection biomolecular reaction microscope carrier and reduce other and use consumptive material; effective efficiency increasing operation biomolecular experiments, and the biomolecular reaction device saving miscellaneous equipment space carries out the method for biological respinse.
In order to achieve the above object, the technical solution adopted in the present invention is: a kind of biomolecular reaction device carries out the method for biological respinse, is characterized in, the method at least comprises the following steps:
(A) biomolecules is provided;
(B) this biomolecules is loaded on a biomolecular reaction microscope carrier;
(C) the biomolecular reaction microscope carrier being mounted with this biomolecules is placed in a reaction solution;
(D) this reaction solution is inserted in the reactive tank in a biological reaction box body to carry out biological respinse, wherein in the reactive tank of this biological respinse box body, this biomolecular reaction microscope carrier is made to maintain fixed position in this reactive tank by a microscope carrier stationary installation, to make when operation experiments mixing is shaken, this biomolecular reaction microscope carrier can not be shifted, and, this biological respinse box body, by one, the envelope box cover of sealing-ring is housed and it forms airtight combination and seals this reactive tank, overflows and evaporation to prevent reaction solution in this reactive tank.
Described biomolecules is gene, DNA or protein.
The medial surface edge described envelope box cover corresponding to this reactive tank has a concave inward structure, and sealing circle is arranged in this concave inward structure.
The side of described envelope box cover and the side of this biological respinse box body be combined with each other with a connection piece.
Described biomolecular reaction microscope carrier is fixed on above this biomolecular reaction microscope carrier, and the identical reactive tank putting into this biological respinse box body.
Described microscope carrier stationary installation coincide be fastened on this biological respinse box body reactive tank in.
Described biomolecular reaction microscope carrier is chip or polymer.
Described envelope box cover comprises fastener, and biological respinse box body corresponds to this fastener and is provided with subassembly, and the fastener of this envelope box cover is buckled in the subassembly of this biological respinse box body to close this reactive tank.
Described sealing-ring is rubber ring body.
Described sealing-ring to be positioned on this envelope box cover and relative to this biological respinse box body.
So; the present invention assists operation biomolecular experiments with aforementioned means; in aforementioned means, all biological experiment flow can be completed; make operation biomolecular experiments tool stability and simplicity; and protection biomolecular reaction microscope carrier can be reached and reduce other use consumptive material; effective efficiency increasing operation biomolecular experiments, and save miscellaneous equipment space.
Accompanying drawing explanation
Fig. 1 is the STRUCTURE DECOMPOSITION schematic diagram of the biomolecular reaction device of the specific embodiment of the invention.
Fig. 2 is the schematic flow sheet that the specific embodiment of the invention carries out biological respinse.
Label declaration:
Biological respinse box body 1 reactive tank 11
Subassembly 12 envelope box cover 2
Concave inward structure 21 web member 22
Fastener 23 sealing-ring 3
Biomolecular reaction microscope carrier 4 microscope carrier stationary installation 5
Step S111 ~ S114.
Embodiment
Refer to shown in Fig. 1 and Fig. 2, be respectively the STRUCTURE DECOMPOSITION schematic diagram of the biomolecular reaction device of the specific embodiment of the invention and the schematic flow sheet carrying out biological respinse of the specific embodiment of the invention.As shown in the figure: the present invention is a kind of method that biomolecular reaction device carries out biological respinse, wherein biomolecular reaction device comprises biological respinse box body 1, envelope box cover 2, sealing-ring 3, biomolecular reaction microscope carrier 4 and microscope carrier stationary installation 5 formed.
Above-mentioned carried biological respinse box body 1 inside has reactive tank 11.
This envelope box cover 2 links with this biological respinse box body 1, and the medial surface edge corresponding to this reactive tank 11 has concave inward structure 21.Wherein, between the side of this envelope box cover 2 and the side of this biological respinse box body 1, be provided with web member 22, to make to combine each other, and form flip-up style biomolecular reaction device.And this envelope box cover 2 arranges fastener 23 in the opposite side relative to web member 22, and this biological respinse box body 1 is to the outside of fastener 23 arranging subassembly 12, the fastener 23 of this envelope box cover 2 is made to be buckled in the subassembly 12 of this biological respinse box body 1, to close this reactive tank 11.
Sealing circle 3 is rubber ring body, to be arranged in the concave inward structure 21 of this envelope box cover 2 and relative to this biological respinse box body 1.
This biomolecular reaction microscope carrier 4 can be chip or polymer, and it is arranged in the reactive tank 11 of this biological respinse box body 1.
This microscope carrier stationary installation 5 is arranged in the reactive tank 11 of this biological respinse box body 1, and is fixed on above this biomolecular reaction microscope carrier 4.
When using, this biomolecular reaction device carries out the method for biological respinse, at least comprises the following step:
Step S111 a: biomolecules is provided, it can be gene, DNA (DNA) or protein;
Step S112: this biomolecules is loaded on this biomolecular reaction microscope carrier 4;
Step S113: the biomolecular reaction microscope carrier 4 being mounted with this biomolecules is placed in a reaction solution (not shown); And
Step S114: this reaction solution is inserted in the reactive tank 11 in this biological respinse box body 1 to carry out biological respinse, this biomolecular reaction microscope carrier 4 can be maintained in this reactive tank 11 with fixed position by this microscope carrier stationary installation 5 in the reactive tank 11 of wherein this biological respinse box body 1, to make when operation experiments mixing is shaken, therefore this biomolecular reaction microscope carrier 4 can not be shifted, and, this biological respinse box body 1 is closed by the envelope box cover 2 that sealing circle 3 is housed or is opened this reactive tank 11, in order to form airtight combination when this envelope box cover 2 covers this biological respinse box body 1, this reactive tank 11 is sealed to make this this biological respinse box body 1 of envelope box cover 2 tight abutment, overflow and evaporation to prevent reaction solution in this reactive tank 11.In this way, the flow process that above discloses forms the method that a brand-new biomolecular reaction device carries out biological respinse.
In a preferred embodiment, the reactive tank 11 of this biological respinse box body 1 inside not only enough reacts for doing with the biomolecules such as gene, DNA or protein, and can coincide and load this biomolecular reaction microscope carrier 4 and this microscope carrier stationary installation 5; Wherein this biomolecular reaction microscope carrier 4 is of moderate size because of it, is the reactive tank 11 can putting into this biological respinse box body 1 in consistent manner, and this microscope carrier stationary installation 5 is then reach closely sealed loading in the reactive tank 11 being fastened on this biological respinse box body 1 in consistent manner.By this, utilize this microscope carrier stationary installation 5 can make this biomolecular reaction microscope carrier 4 fixed position in this biological respinse box body 1, not because adding reaction reagent or cleaning action and moving, make operation biomolecular experiments tool stability and simplicity.And, this biological respinse box body 1 is connected with this envelope box cover 2 through this web member 22, this biological respinse box body 1 can be covered with this subassembly 12 via this fastener 23, utilize the sealing-ring 3 be equipped with in the concave inward structure 21 at this envelope box cover 2 edge, when can assist to cover this biological respinse box body 1, this envelope box cover 2 and the clearance gap of this biological respinse box body 1 are filled up present edges close to coincide shape, to reach sealing Anti-spill, prevent the reaction solution in this reactive tank 11 from overflowing and evaporation, and be maintained original volume and concentration.Therefore; the present invention aforementioned means can assist operation biomolecular experiments; in aforementioned means, all biological experiment flow can be completed; effectively can improve the nothing normalization of conventional plastic container, inconvenience and be not suitable for the shortcomings such as microscope carrier; protection biomolecular reaction microscope carrier can be reached and reduce other and use consumptive material; effective efficiency increasing operation biomolecular experiments, and save miscellaneous equipment space.
In sum; a kind of biomolecular reaction device of the present invention carries out the method for biological respinse; effectively can improve the various shortcoming of prior art; in biomolecular reaction device, all biological experiment flow can be completed; reach protection biomolecular reaction microscope carrier and reduce other and use consumptive material; effective efficiency increasing operation biomolecular experiments; and save miscellaneous equipment space; and then make generation of the present invention more progressive, more practical, more can meet user institute palpus; indeed meet the important document of application for a patent for invention, propose patent application in accordance with the law.
But the above, be only preferred embodiment of the present invention, when not limiting scope of the invention process with this; Therefore all simple equivalences done according to the present patent application the scope of the claims and description of the invention content change and modify, and all should still remain within the scope of the patent.
Claims (10)
1. biomolecular reaction device carries out a method for biological respinse, it is characterized in that, the method at least comprises the following steps:
(A) biomolecules is provided;
(B) this biomolecules is loaded on a biomolecular reaction microscope carrier;
(C) the biomolecular reaction microscope carrier being mounted with this biomolecules is placed in a reaction solution;
(D) this reaction solution is inserted in the reactive tank in a biological reaction box body to carry out biological respinse, wherein in the reactive tank of this biological respinse box body, this biomolecular reaction microscope carrier is made to maintain fixed position in this reactive tank by a microscope carrier stationary installation, to make when operation experiments mixing is shaken, this biomolecular reaction microscope carrier can not be shifted, and, this biological respinse box body, by one, the envelope box cover of sealing-ring is housed and it forms airtight combination and seals this reactive tank, overflows and evaporation to prevent reaction solution in this reactive tank.
2. biomolecular reaction device as claimed in claim 1 carries out the method for biological respinse, and its thing is levied and is, described biomolecules is gene, DNA or protein.
3. biomolecular reaction device as claimed in claim 1 carries out the method for biological respinse, and its thing is levied and is, the medial surface edge described envelope box cover corresponding to this reactive tank has a concave inward structure, and sealing circle is arranged in this concave inward structure.
4. biomolecular reaction device as claimed in claim 1 carries out the method for biological respinse, and its thing is levied and is, the side of described envelope box cover and the side of this biological respinse box body be combined with each other with a connection piece.
5. biomolecular reaction device as claimed in claim 1 carries out the method for biological respinse, and its thing is levied and is, described biomolecular reaction microscope carrier is fixed on above this biomolecular reaction microscope carrier, and the identical reactive tank putting into this biological respinse box body.
6. biomolecular reaction device as claimed in claim 1 carries out the method for biological respinse, and its thing is levied and is, described microscope carrier stationary installation coincide be fastened on this biological respinse box body reactive tank in.
7. biomolecular reaction device as claimed in claim 1 carries out the method for biological respinse, and its thing is levied and is, described biomolecular reaction microscope carrier is chip or polymer.
8. biomolecular reaction device as claimed in claim 1 carries out the method for biological respinse, its thing is levied and is, described envelope box cover comprises fastener, and biological respinse box body corresponds to this fastener and is provided with subassembly, the fastener of this envelope box cover is buckled in the subassembly of this biological respinse box body to close this reactive tank.
9. biomolecular reaction device as claimed in claim 1 carries out the method for biological respinse, and its thing is levied and is, described sealing-ring is rubber ring body.
10. biomolecular reaction device as claimed in claim 1 carries out the method for biological respinse, and its thing is levied and is, described sealing-ring to be positioned on this envelope box cover and relative to this biological respinse box body.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN201410261281.6A CN105296465A (en) | 2014-06-13 | 2014-06-13 | Biological reaction method of biological molecule reaction device |
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CN201410261281.6A CN105296465A (en) | 2014-06-13 | 2014-06-13 | Biological reaction method of biological molecule reaction device |
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CN105296465A true CN105296465A (en) | 2016-02-03 |
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CN201410261281.6A Pending CN105296465A (en) | 2014-06-13 | 2014-06-13 | Biological reaction method of biological molecule reaction device |
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Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1325026A (en) * | 2001-07-10 | 2001-12-05 | 重庆大学 | Piezoelectric biological chip miniflow detector |
CN2793088Y (en) * | 2005-05-30 | 2006-07-05 | 西安交通大学 | Biological chip hybridization reacting box |
CN1948479A (en) * | 2006-11-09 | 2007-04-18 | 博奥生物有限公司 | Biochip hybridizing box |
CN201153645Y (en) * | 2008-03-04 | 2008-11-26 | 王天强 | Sealed case |
CN203947099U (en) * | 2014-06-13 | 2014-11-19 | 康隽生物科技股份有限公司 | Biomolecular reaction device |
-
2014
- 2014-06-13 CN CN201410261281.6A patent/CN105296465A/en active Pending
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1325026A (en) * | 2001-07-10 | 2001-12-05 | 重庆大学 | Piezoelectric biological chip miniflow detector |
CN2793088Y (en) * | 2005-05-30 | 2006-07-05 | 西安交通大学 | Biological chip hybridization reacting box |
CN1948479A (en) * | 2006-11-09 | 2007-04-18 | 博奥生物有限公司 | Biochip hybridizing box |
CN201153645Y (en) * | 2008-03-04 | 2008-11-26 | 王天强 | Sealed case |
CN203947099U (en) * | 2014-06-13 | 2014-11-19 | 康隽生物科技股份有限公司 | Biomolecular reaction device |
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Application publication date: 20160203 |