KR20120059151A

KR20120059151A - Culture tray

Info

Publication number: KR20120059151A
Application number: KR1020100120788A
Authority: KR
Inventors: 고정문
Original assignee: (주)나비바이오텍
Priority date: 2010-11-30
Filing date: 2010-11-30
Publication date: 2012-06-08

Abstract

The present invention relates to a culture tray, and particularly discloses a micro culture tray that can open and close multiple channels at the inlet and outlet at the same time and is easy to handle by minimizing volume.
In the present invention, a channel for culturing, and a culture tray having an inlet and an outlet formed at both ends of the channel, wherein the channel is formed between an upper plate formed with the inlet and an outlet, and a lower plate superimposed on one side of the upper plate. The inlet and the outlet are respectively located on the upper surface of the upper surface that is in contact with each other, the hinge is coupled to one side of the top plate and rotated based on the hinge axis to cover or open the inlet and outlet formed on the top plate, and the other side of the top plate By providing a culture tray equipped with a label space for recording, it is possible to incubate a plurality of samples in a minimized culture tray, thereby minimizing the economic burden and enabling efficient handling and management.

Description

Culture tray {CULTURE TRAY}

The present invention relates to a culture tray, and more particularly, to a micro culture tray that can be opened and closed at the same time inlet and outlet of multiple channels, and is easy to handle by minimizing volume.

As is well known, in general, the cells and the culture medium in the culture dish in a classic method for culturing the cells, and then covered with a cover and left for about 3 days to allow cell culture to be carried out. Dispense the mixture, stir evenly, and tilt to extract the washing solution.

Then, in order to detach the cells from the culture dish, an enzyme such as trypsin (TRYPSIN) or accutase (ACCUTASE) was added to the CO2 incubator, incubated for 1 to 3 minutes, and then observed under a microscope to see if the cells were removed from the culture dish. Check and if it does not come off, apply some impact to make it go away. Subsequently, the contents of the culture dish are put together with the culture solution in a centrifuge, rotated, and centrifuged to remove the separated supernatant, thereby allowing the cultured cells to be taken out.

This series of processes has a problem that the work efficiency is low because the process of the multiple steps must be carried out with careful manual work.

In addition, in the related art, since the process must be performed using an open plane such as a culture dish, the amount of various solutions or enzymes must be sufficiently used to work in the open plane, thereby increasing the cost burden.

In order to solve this problem, a culture tray has been proposed, and a representative example thereof may be viewed by US Patent No. 7,517,499 (name of the invention: Flow Chamber; hereinafter referred to as 'quotation invention').

This cited invention is made of plastic provided with an object carrier for microscopic observation of one or more channels 4, as shown in FIG. 1, wherein the channels described above are 0.01-20 mm wide and 0.01-5 mm high. One liquid container 3, 3 'provides a flow chamber that is structured in communication with the inlet and outlet apertures 6, 6' of the channel, respectively. The bottom of this chamber and the cover covering the receptors 3, 3 'are formed and functionalized with high grade plastic material.

On the other hand, this cited invention has the trouble of covering and removing the cover of the liquid receptors (3, 3 ') at the inlet and the outlet for each process of injecting and recovering the sample, and can be covered and removed by hand. Since it should be manufactured to a size that is enough, it is difficult to produce smaller than the appropriate size, there is a problem that is inadequate in the culture of a very small amount of sample.

In addition, the cited invention is to be provided with a separate flow chamber (flow chamber) for each sample, when there is a problem in that the purchase cost increases as well as cumbersome handling and storage when a large number of sample cultures should be carried out.

Cited Invention: US Patent No. 7,517,499

The purpose of the present invention is to remove the receptor protruding from the inlet and outlet of the channel to solve this problem, and to form the plate so that the inlet and outlet of the channel can be covered or opened by a cover rotated by the hinge axis In addition to miniaturization, it is possible to form a plurality of the channels, form a division mark on the plurality of channels, and provide a culture tray for easy handling because a space for describing a sample name for each channel is provided on one side of the main body.

The present invention provides a channel for culturing and an inlet and an outlet formed at both ends of the channel to achieve the above object,

The channel is formed between the upper plate formed with the inlet and the outlet, and the lower plate superimposed on one surface of the upper plate, wherein the inlet and the outlet are respectively located on the upper plate surface where both ends of the channel contact each other, and one side and the hinge of the upper plate. The cover is rotated about the hinge axis to cover or open the inlet and the outlet formed on the upper plate, and a marking space for recording on the other side of the upper plate.

In this way, the present invention is capable of culturing a plurality of samples in a minimized culture tray, thereby minimizing the economic burden and enabling efficient handling and management.

In addition, the present invention is a cover of a size that can be handled by hand and the corresponding container is unnecessary, replaced by a rotating plate cover, so that the volume can be minimized to be miniaturized, so a very small amount In the case of the sample also has a useful effect that can be cultured.

In addition, the present invention is to be able to open and close a plurality of channel inlet and outlet by opening and closing one plate-like cover is to be easy to handle and efficient work is possible.

1 is a cross-sectional view showing the configuration of the cited invention.
Figure 2 is a perspective view illustrating a specific embodiment of the culture tray according to the present invention.
3, 4 is an exploded perspective view of the culture tray according to the present invention.
5 is a cross-sectional view of the culture tray according to the present invention.
Figure 6 is an exploded perspective view of another embodiment of the culture tray according to the present invention.
Figure 7 is a cross-sectional view of another embodiment of the culture tray according to the present invention.

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings.

The overall configuration of a specific example of the culture tray according to the present invention is shown in FIG. In the present exemplary embodiment, the culture tray in which the two channels 10 are formed is illustrated, but one channel 10, three channels 10, four channels 10, ..., 16 channels 10, etc. It is not limited to the number.

3 and 4 illustrate exploded perspective views of different components in a direction of looking at the culture tray according to the present invention in order to show each component of the embodiment shown in FIG. 2 in detail.

As can be seen from the present invention, in the culture tray 10 and the culture tray formed with an inlet 211 and an outlet 212 at both ends of the channel 10,

The channel 10 is formed between the upper plate 200 formed with the inlet 211 and the outlet 212 and the lower plate 300 superimposed on one surface of the upper plate 200.

The inlet 211 and the outlet 212 are respectively located on the surface of the upper plate 200 in contact with both ends of the channel 10,

A cover 220 may be provided that is hinged to one side of the upper plate 200 and rotates based on the hinge axis to cover or open the inlet 211 and the outlet 212 formed on the upper plate 200.

If you explain this in more detail for each component,

The above-described channels 10 may be formed in plural between the upper plate 200 and the lower plate 300, and both ends of each channel 10 may be formed at each inlet 211 and outlet through the upper plate 200. 212) is connected.

3 and 4, the inlet 211 and the outlet 212 are connected to both ends of the channel 10, respectively, and the cover 200 is formed on one side of the upper plate 200. 220 is hinge-coupled, the other side of the upper plate 200 is provided with a marking space 230 for recording.

Here, the marking space 230 is formed to protrude so that the outer surface of the marking space 230 and the outer surface of the cover 220 are located on the same plane, so that the plurality of channels from the cover 220 to the marking space 230. (10) It is possible to display a distinctive marker for distinguishing each one, and to describe the sample name and the experiment name for each channel 10. It will be possible. This can be seen in Figure 5 showing a cross section of the culture tray according to the present invention.

In addition, the cover 220, as shown in Figures 3 and 4, the anti-slip projections 221 may be formed protruding on the side, the leakage preventing packing 222 around the surface in contact with the top plate 200 It can be formed, it is easy to open and close the cover 220 without slipping with one hand, and when the cover 220 is closed, it is sealed with the leakage preventing packing 222 to prevent leakage of the sample and the like. In addition, by simply closing and opening the cover 220, all inlets 211 and outlets 212 of each channel 10 can be opened and closed at the same time to facilitate the culture operation.

Furthermore, the liquid receptor protruding from the inlet 211 and the outlet 212 of the channel 10 and a plurality of covers separately covering each of these liquid receptors are removed and the inlet 211 and the outlet 212 of the channel 10 are removed. The plate-shaped cover 220 directly covering) is configured to minimize the thickness of the culture tray.

Here, the leakage preventing packing 222 of the cover 220 is a plurality of packings 222 ′ inserted into each of the inlet 211 and the outlet 212 of the channel 10, as shown in FIGS. 6 and 7. ) May be formed to protrude on one surface of the cover 220 in contact with the top plate 200.

In addition, the upper plate 200, the cover 220 and the lower plate 300 is polydimethylsiloxane (poly (dimethylsiloxane), PDMS), polymethyl methacrylate (polymethylmethacrylate (PMMA), polyacrylates, polycarbonate (polycarbonates), polycyclic olefins, polyimides, polyurethanes, or a synthetic polymer material thereof, but the culture tray itself covered with the cover 220 by a microscope It is desirable to form transparent so that it can be observed.

The culture tray according to the present invention is hinged to the upper plate 200, the cover 220 for opening and closing the inlet 211 and the outlet 212 of the channel 10 by rotating based on the hinge axis of all the channels 10 Opening the inlet 211 and the outlet 212 at the same time and put different cells, culture medium, sample, etc. into the respective channels 10 through the respective inlet 211 and again rotate the cover 220 to reverse all By simply closing both the inlet 211 and the outlet 212 of the channel 10 at the same time, the culture preparation is completed,

The upper plate 200, the cover 220, and the lower plate 300 are formed of a transparent polymer material having a very high light transmittance, and thus the cover 220 is closed so that the leakage preventing packings 222 and 222 ′ may be used to form cells, culture fluids, and samples. By cultivating the culture tray itself in a state that prevents leakage of the back directly to the microscope, you can easily observe the culture state and results, etc. without fear of leakage.

Instead of opening the inlet 211 and the outlet 212 of each channel 10 to extract the cells in which the culture is completed, the cover 220 is rotated about the hinge axis and opened to open all the channels 10. The inlet 211 and the outlet 212 can be opened at the same time, so that enzymes such as trypsin or accutase, which release the cells which have been incubated through the inlet 211 of the respective channels 10, are introduced. By using the outlet 212 of each channel 10 will be able to reduce the elaborate manual process of taking out the cells completed culture.

As described above, the present invention is not limited to the above-described embodiments, but can be variously changed within the spirit and concept of the present invention.

10: channel 200: top plate
211: entrance 212: exit
220: cover 221: protrusion
222, 222 ': Packing 230: Marker space
300: lower plate

Claims

In the culture tray and the culture tray formed inlet and outlet at both ends of the channel,
The channel is formed between the upper plate formed with the inlet and the outlet, and the lower plate superimposed on one surface of the upper plate,
The inlet and the outlet are respectively located on the upper surface of the channel in contact with both ends of the channel,
And a cover coupled to one side of the top plate and pivoted about a hinge axis to cover or open an inlet and an outlet formed on the top plate.

The method of claim 1,
The top plate is culture tray, characterized in that having a marking space for recording on the other side.

The method of claim 1,
The cover is a culture tray, characterized in that the anti-slip projection protruding on the side.

The method of claim 1,
The cover is a culture tray, characterized in that the leakage preventing packing is formed around the one surface in contact with the top plate.

The method of claim 1,
The cover is culture tray, characterized in that the packing is formed in the inlet and outlet formed on the top plate to prevent leakage.

The method of claim 1,
The cover and the bottom plate are made of polydimethylsiloxane (PDMS), polymethylmethacrylate (PMMA), polyacrylates, polycarbonates, polycyclic olefins, A culture tray, characterized in that formed by transparently using any one of polyimides, polyurethanes (polyurethanes).