CN1325026A - Piezoelectric biological chip miniflow detector - Google Patents
Piezoelectric biological chip miniflow detector Download PDFInfo
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- CN1325026A CN1325026A CN 01108613 CN01108613A CN1325026A CN 1325026 A CN1325026 A CN 1325026A CN 01108613 CN01108613 CN 01108613 CN 01108613 A CN01108613 A CN 01108613A CN 1325026 A CN1325026 A CN 1325026A
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- biological chip
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Abstract
The piezoelectric biochip microflow detection device comprises piezoelectric biochip which is placed in a box and formed into a closed detection cell, on the box body there are inlet pipe and outlet pipe of the detection cell. Said invention is easy to implement automation, simple in structure, less in required sample, convenient for practical applications of clinical examination and environmental monitoring, can simultaneously determine several target molecules in liquid sample, and is applicable to analysis and detection in the fields of chemistry, physics, clinical medicine, pharmacy and environmental sciences.
Description
Technical field
The present invention relates to biological and chemical analysis instrument technical field.The circulation type piezoelectric biological chip pick-up unit of the detection working fluid sample that particularly a kind of microbody is long-pending.
Background technology
Have the pick-up unit that liquid phase is used a plurality of piezoelectric sensors that is used for of report, as " AnalyticaChimica Acta " 1991, Vol.251, No.1, the article of " piezoelectric quartz sensor is used for the solution multicomponent analysis " that p143-148 delivers, disclose and adopted four piezoelectric sensors, response to different component there are differences under different pH based on each sensor, realized in the water adjacent, measure in the time of metacresol, all be to constitute by a plurality of independently quartz-crystal resonators and oscillatory circuit thereof, but this device can not detect the liquor sample that flows, and device is complicated, the cost height is not easy to practical application.The report that does not also relate at present circulation type piezoelectric biological chip pick-up unit.
Piezoelectric biological chip, as Chinese patent 991174402 " MIniature piezoelectric resonant sensor array chip " and the disclosed technology of Chinese patent 001131109 " in-situ biochip and preparation method thereof ", be that a plurality of piezoelectric biological sensors are integrated on the chip, when being connected with matching used oscillatory circuit, each electrode on the chip forms independently resonance detecting unit, measure the response signal of each detecting unit such as the resonance frequency of device, the acoustic-electric impedance spectrum, frequency spectrum or phase place etc., can obtain the composition of relevant target components or multicomponent mixture system, the one or more dimensions information of proterties obtains the comprehensive of target, dynamically, description real-time or on the throne.This piezoelectric biological chip is applicable to the analyzing and testing in fields such as chemistry, biology, pharmacy, clinical medicine and environmental science.
Summary of the invention
The purpose of this invention is to provide a kind ofly can detect the flowing liquid sample, easily be automated, simple in structure, required sample is few, be convenient to practical applications such as clinical examination and environmental monitoring, and can measure the piezoelectric biological chip miniflow detector of plurality of target molecule in the liquor sample simultaneously.
For reaching above-mentioned purpose, the present invention is on the basis of existing piezoelectric biological chip, employing is installed in piezoelectric biological chip in the sealing box body of liquid in-out passage, this device can utilize the liquid in-out passage that liquor sample is flowed on piezo chips, thereby realizes the liquor sample that flows is detected.
Piezoelectric biological chip miniflow detector of the present invention (referring to accompanying drawing) comprises piezoelectric biological chip (1), and piezoelectric biological chip places box body and constitutes enclosed detection cell (3), has detection cell to lead to outer at least one pipeline (5) on the box body.
Above-mentioned box body has pedestal (8) and the cover plate that is connected with pedestal (4), the middle part of pedestal is the close solid above-mentioned piezoelectric biological chip (1) of stage of concave shaped, form above-mentioned detection cell (3) between one side of at least one microelectrode (2) that bioprobe is arranged of piezoelectric biological chip and the cover plate (4), between the opposite side that common electrode (10) is arranged of piezoelectric biological chip and pedestal cavity (9) is arranged, microelectrode of piezoelectric biological chip (2) and common electrode (10) have the lead-in wire (6,7) that leads to the external world, and above-mentioned pipeline (5) is on cover plate (4).
Above-mentioned piezoelectric biological chip (1) is plain film shape, and its unilateral circle or ellipse or limit of being shaped as is counted the n limit shape of n 〉=3, and above-mentioned box body is the right cylinder bodily form, and the unilateral shape of the transverse shape of this cylinder and piezoelectric biological chip matches.
Above-mentioned bioprobe is that said bioprobe is at least a in the biomolecule, cell, microorganism of nucleic acid, protein, peptide.
Use when of the present invention, the present invention is fitted on the detector, the flowing liquid sample from importing, flow out from two pipelines respectively, is detected by usual way.
The present invention has following advantage and effect compared with the prior art.
One, the dress piezoelectric biological chip constitutes the box body structure in flow detection pond in of the present invention, can realize mobile liquor sample is detected, so be easy to realize the robotization that detects.
Two, of the present invention simple in structure, volume is small and exquisite, required sample is few during detection, easy to use flexibly, make things convenient for practical applications such as clinical examination and environmental monitoring.
Three, the piezoelectric biological chip structure that matches with box body of the present invention can be measured the plurality of target molecule in the liquor sample simultaneously.
Be applicable to the analyzing and testing in fields such as chemistry, biology, pharmacy, clinical medicine and environmental science.
Below, with embodiment and accompanying drawing thereof the present invention is further described again.
Description of drawings
Fig. 1 is the structural representation of a kind of little circulation type piezoelectric biological chip pick-up unit of the present invention.
Fig. 2 is the A-A sectional view of Fig. 1.
Fig. 3 is the B-B cut-open view of Fig. 1.
Embodiment
Embodiment 1
A kind of piezoelectric biological chip miniflow detector of the present invention as shown in drawings, is made of piezoelectric biological chip and box body.
Above-mentioned piezoelectric biological chip 1, adopt common structure, the microelectrode array that at least one microelectrode 2 is arranged by piezoelectric patches, piezoelectric patches one lateral plate face, with the unilateral common electrode 10 of opposite side and be fixed on microelectrode 2 lip-deep bioprobe (not shown)s and constitute, common electrode and microelectrode are drawn by lead-in wire 6,7 separately respectively.Above-mentioned piezoelectric patches quartz crystal slice also can select for use piezoelectrics such as piezoelectric ceramics or piezoelectric polyvinylidene fluoride film to make.Above-mentioned microelectrode 2, common electrode 10 and go between and 6,7 select gold copper-base alloy for use also can select for use conductive materials such as silver or aluminium to make.Piezoelectric patches and common electrode, microelectrode are sheet shape, and its unilateral circle or ellipse or limit of being shaped as is counted the n limit shape of n 〉=3, promptly can be polygons such as triangle, quadrilateral, pentagon, and present embodiment adopts quadrilateral.Above-mentioned bioprobe is selected nucleic acid for use, also can select biomolecule such as protein, peptide for use, at least a in the biomaterials such as cell, microorganism.
Above-mentioned box body is the right cylinder bodily form.The unilateral shape of the transverse shape of cylinder and piezoelectric biological chip matches.Because the piezoelectric biological chip of present embodiment adopts rectangle, so box body adopts cubic vertical cyclindrical shape, constitute by the base and cover plate of cuboid, all select pottery for use, also can select for use insulating material such as plastics or glass to make.Said base 8 is the recessed box-like that is formed with the rectangular cavity of stage in middle part.On the wall of the chamber of cavity,, open the lead-in wire groove of the lead-in wire groove that is shaped on common electrode and each microelectrode corresponding to the position of common electrode and little shape electrode.Above-mentioned piezoelectric biological chip 1 is placed on the interior stage of said base, respectively form a cavity in both sides up and down at piezoelectric biological chip, the size of upside cavity is advisable be able to hold piezoelectric biological chip, stage is a bit larger tham the thickness of piezoelectric biological chip to the height of pedestal upper surface, piezoelectric biological chip has the cavity 9 of the hypopleurite face of common electrode 10 towards downside, and is advisable not contact common electrode 10 at the bottom of chamber wall and the chamber; Stick with glue agent or hot pressing or other method, with piezoelectric biological chip 1 deadend be fixed on the stage, and 6 and 7 each microelectrode 2 and common electrode 10 drawn one by one by going between.
Above-mentioned cover plate 4 is plate shape, and a pipeline 5 that leads to the L shaped tubulose of medial surface from end face is respectively arranged at the cover plate two ends.Stick with glue agent or hot pressing or other method, the medial surface of cover plate and the table top of pedestal are closely linked, like this at piezoelectric biological chip 1 and 4 detection cells 3 that form the inner chamber shape of a sealing of cover plate, thereby a lateral plate face of the microelectrode that bioprobe is arranged 2 of piezoelectric biological chip 1 is arranged in detection cell, and on detection cell and the cover plate mutually away from two pipelines 5 communicate, the flowing liquid sample can be by flowing into by two pipelines respectively and the outflow detection cell.The volume of this detection cell is placed piezoelectric biological chip by pedestal stage determines to the gap of the thickness of the height of its upper surface and piezoelectric biological chip and the area of detection cell.When the size one of piezoelectric biological chip regularly, can adjust the volume of detection cell to the height of upper surface by adjusting the pedestal stage, be that the area of 0.10mm, detection cell is 15 * 7mm as the thickness of piezoelectric biological chip
2, when the pedestal stage when the height of upper surface is respectively 0.20mm, 0.14mm and 0.11mm, the volume of detection cell 3 is respectively 10.5 μ L, 4.2 μ L and 1.0 μ L.Therefore, the height dimension precision just can make the volume of detection cell be controlled in several microlitres below 0.01mm, obtains piezoelectric biological chip miniflow detector of the present invention.
When using present embodiment, this miniflow detector is fitted in the common detector, and the lead-in wire 6,7 of each microelectrode 2 and common electrode 10 connected with detector respectively, make the flowing liquid sample flow into, flow out detection cell 3 from two pipelines 5 continuously, in the flowing of liquor sample, detect.
Claims (4)
1, piezoelectric biological chip miniflow detector comprises piezoelectric biological chip (1), it is characterized in that piezoelectric biological chip places box body and constitutes enclosed detection cell (3), has detection cell to lead to outer at least one pipeline (5) on the box body.
2, piezoelectric biological chip miniflow detector according to claim 1, it is characterized in that said box body has pedestal (8) and the cover plate that is connected with pedestal (4), the middle part of pedestal is the close solid above-mentioned piezoelectric biological chip (1) of stage of concave shaped, form above-mentioned detection cell (3) between one side of at least one microelectrode (2) that bioprobe is arranged of piezoelectric biological chip and the cover plate (4), between the opposite side that common electrode (10) is arranged of piezoelectric biological chip and pedestal cavity (9) is arranged, microelectrode of piezoelectric biological chip (2) and common electrode (10) have the lead-in wire (6 that leads to the external world, 7), said pipeline (5) is on cover plate (4).
3, piezoelectric biological chip miniflow detector according to claim 1 and 2, it is characterized in that said piezoelectric biological chip (1) is plain film shape, its unilateral circle or ellipse or limit of being shaped as is counted the n limit shape of n 〉=3, said box body is the right cylinder bodily form, and the unilateral shape of the transverse shape of this cylinder and piezoelectric biological chip matches.
4, piezoelectric biological chip miniflow detector according to claim 3 is characterized in that said bioprobe is at least a in the biomolecule, cell, microorganism of nucleic acid, protein, peptide.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 01108613 CN1325026A (en) | 2001-07-10 | 2001-07-10 | Piezoelectric biological chip miniflow detector |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 01108613 CN1325026A (en) | 2001-07-10 | 2001-07-10 | Piezoelectric biological chip miniflow detector |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004083863A1 (en) * | 2003-03-04 | 2004-09-30 | Chengdu Kuachang Medical Industrial Limited | An integrating analysis chip with minimized reactors and its application |
| WO2004111626A1 (en) * | 2003-06-18 | 2004-12-23 | Shizheng Chen | A mass amplified piezoelectric biochip |
| WO2005073363A1 (en) * | 2003-09-30 | 2005-08-11 | Chengdu Kuachang Medical Industrial Limited | An analysis chip, an apparatus comprising the chip, the chip test kit and the measurement method thereof |
| CN101915793A (en) * | 2010-08-18 | 2010-12-15 | 中国科学院半导体研究所 | Microelectrode array and microchannel integrated sensor structure and manufacturing method thereof |
| CN102279255A (en) * | 2010-10-20 | 2011-12-14 | 重庆工商大学 | Mechanical dynamic regulation and control chip for single cell total environment |
| CN102980826A (en) * | 2011-09-03 | 2013-03-20 | 崔学晨 | Quartz crystal microbalance detection cell |
| CN105296465A (en) * | 2014-06-13 | 2016-02-03 | 康隽生物科技股份有限公司 | Biological reaction method of biological molecule reaction device |
| CN107570482A (en) * | 2017-07-06 | 2018-01-12 | 天津大学 | The removal device and method of the non-specific adsorption thing at interface |
| CN107817318A (en) * | 2017-11-23 | 2018-03-20 | 昌微系统科技(上海)有限公司 | A kind of mounting box and its application method for sensor chip |
| WO2018209923A1 (en) * | 2017-05-15 | 2018-11-22 | 中国科学院苏州生物医学工程技术研究所 | Clotting test paper and method for preparing piezoelectric sensor chip thereof |
| CN111351848A (en) * | 2020-03-19 | 2020-06-30 | 山东科技大学 | Preparation method of sensor, sensor and detection method of sensor |
-
2001
- 2001-07-10 CN CN 01108613 patent/CN1325026A/en active Pending
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004083863A1 (en) * | 2003-03-04 | 2004-09-30 | Chengdu Kuachang Medical Industrial Limited | An integrating analysis chip with minimized reactors and its application |
| WO2004111626A1 (en) * | 2003-06-18 | 2004-12-23 | Shizheng Chen | A mass amplified piezoelectric biochip |
| WO2005073363A1 (en) * | 2003-09-30 | 2005-08-11 | Chengdu Kuachang Medical Industrial Limited | An analysis chip, an apparatus comprising the chip, the chip test kit and the measurement method thereof |
| CN101915793A (en) * | 2010-08-18 | 2010-12-15 | 中国科学院半导体研究所 | Microelectrode array and microchannel integrated sensor structure and manufacturing method thereof |
| CN102279255B (en) * | 2010-10-20 | 2013-08-28 | 重庆工商大学 | Mechanical dynamic regulation and control chip for single cell total environment |
| CN102279255A (en) * | 2010-10-20 | 2011-12-14 | 重庆工商大学 | Mechanical dynamic regulation and control chip for single cell total environment |
| CN102980826A (en) * | 2011-09-03 | 2013-03-20 | 崔学晨 | Quartz crystal microbalance detection cell |
| CN105296465A (en) * | 2014-06-13 | 2016-02-03 | 康隽生物科技股份有限公司 | Biological reaction method of biological molecule reaction device |
| WO2018209923A1 (en) * | 2017-05-15 | 2018-11-22 | 中国科学院苏州生物医学工程技术研究所 | Clotting test paper and method for preparing piezoelectric sensor chip thereof |
| CN107570482A (en) * | 2017-07-06 | 2018-01-12 | 天津大学 | The removal device and method of the non-specific adsorption thing at interface |
| CN107817318A (en) * | 2017-11-23 | 2018-03-20 | 昌微系统科技(上海)有限公司 | A kind of mounting box and its application method for sensor chip |
| CN107817318B (en) * | 2017-11-23 | 2023-11-10 | 宁卡赛科技(上海)有限公司 | Mounting box for sensor chip and use method thereof |
| CN111351848A (en) * | 2020-03-19 | 2020-06-30 | 山东科技大学 | Preparation method of sensor, sensor and detection method of sensor |
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