CN110174454A - A kind of electrochemical sensor and preparation method thereof of microtrabeculae high throughput - Google Patents
A kind of electrochemical sensor and preparation method thereof of microtrabeculae high throughput Download PDFInfo
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- CN110174454A CN110174454A CN201910527632.6A CN201910527632A CN110174454A CN 110174454 A CN110174454 A CN 110174454A CN 201910527632 A CN201910527632 A CN 201910527632A CN 110174454 A CN110174454 A CN 110174454A
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
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Abstract
The present invention provides a kind of electrochemical sensor and preparation method thereof of microtrabeculae high throughput, the sensor is assembled into several micro-pillar arrays by simply plug, realize the high-flux electric chemical sensitisation of microlayer model, the sensor includes microtrabeculae drop administrative unit and electrochemistry transmission unit.Wherein drop administrative unit is the small-sized microtrabeculae comprising three-electrode system, and the adhesion strength on surface can fix micro drop；Electrochemistry transmission unit is the integrated circuit board with array electrode hole, and the electrode wires and circuit board of microtrabeculae end are directly connected to form micro-pillar array, and circuit board is integrated by internal wiring and realizes high-flux electric chemical detection.This microtrabeculae sensor has the detection scale of construction small, can multifunction modification, signal easily detect and the high-throughput characteristics such as integrate are, it can be achieved that trace body fluid ionic monitoring, the more marker detections of trace and high-flux medicaments sifting etc. are expected to that conventional commercial orifice plate is replaced to become high throughput analysis senser element of new generation.
Description
Technical field
The present invention relates to devices to construct and chemical sensitisation field, in particular to a kind of electrochemical sensor of microtrabeculae high throughput
And preparation method thereof.
Background technique
High-throughput detection platform greatly reduces detection error by the integration output of multi signal, shortens detection time.
Staff realizes food-safe property using array detection platform and analyzes in recent years, the detection of the more markers of biology, raw
Object oxicity analysis and high-flux medicaments sifting etc..The array detection platform of current most maturing is multifunctional orifice plate, passes through battle array
High-throughput microlayer model analysis is realized in columnization micro chamber and mechanization titration.This orifice plate imprisons drop in micro chamber, is unfavorable for
The evaporation of microlayer model is stirred and transfer, simultaneously because orifice plate itself does not have sensing capabilities, also stops to the analysis of inner droplets
In simple fluorescence and colorimetric detection, these methods be not only easy by outside fluctuation interference but also can introduce toxic light emitting molecule, only
It can accomplish semi-quantitative analysis, this just significantly limits the development and practical application of high-throughput sensing platform.
Electrochemical sensing technology is due to its strong, high sensitivity of selectivity, and detection speed is fast, and equipment is simple, and testing cost is low
The advantages that be widely used in environmental monitoring, food safety and drug screening field.There is scientific research personnel's trial in recent years
By Electrochemical Detection and array Platform integration to together, using the microelectrode of the methods of photoetching preparation array, then with close and distant
Moisture film or polymer microcavity will test the high-throughput detection that liquid separates microlayer model.This method had both solved Conventional electrochemical inspection
Survey the big problem of the scale of construction, and realize precisely quantitative Electrochemical Detection, greatly pushed microarray platform in high-precision and
The application of high accuracy biochemistry detection.
Summary of the invention
To solve the above-mentioned problems, the present invention provides a kind of electrochemical sensor and preparation method thereof of microtrabeculae high throughput,
The sensor design is flexible, at low cost, have can functional modification, detection the scale of construction it is small, it is reusable and be convenient for high pass
Amount integration.
A kind of electrochemical sensor of microtrabeculae high throughput, the sensor includes microlayer model fixed test unit and electric signal
Transmission unit, for the microlayer model fixed test unit for carrying out electrical signal detection and fixed micro drop, the microlayer model is solid
Determine detection unit connection electric signal transmission unit；
Further, the microlayer model fixed test unit is the small-sized microtrabeculae of several independents, inside each small-sized microtrabeculae
It include three-electrode system, electrical signal detection is carried out by the three-electrode system, and the small-sized micro-post surface adhesion strength can be consolidated
Fixed micro drop；
Further, the electric signal transmission unit be integrated circuit board, the integrated circuit board by itself electrode hole with
The electrode wires plug-in connection outstanding of microtrabeculae end, the microtrabeculae of independent is integrated together, realizes the high throughput in microlayer model
Electrochemical Detection；
Further, for the three-electrode system using Au-Ag-Pt metal wire as three-electrode system, silver is used as reference electrode, and platinum is made
For to electrode, Jin Zuowei working electrode, metal wire outer layer all has Teflon thin layer in the three-electrode system, avoids three kinds of gold
Belong to contacting with each other between line and causes short circuit；
Further, the three-electrode system can realize that Conventional electrochemical detects, and in the three-electrode system, be while meeting
Special detection analyte detection and high-sensitivity analysis demand, on the basis of gold and silver platinum filament is as three-electrode system, on the electrode into
The multi-functional modification of row, to improve sensitivity and the practicability of small-sized microtrabeculae sensor, the multi-functional modification is repaired including gold surface
Adorn the dendritic gold of nanoscale, graphene absorption and carbon nanotube deposition；
Further, the small-sized microtrabeculae of several independents is 8 × 12 array distributions, and the integrated circuit board has 8 × 12
Array socket unit respectively corresponds the small-sized microtrabeculae of 8 × 12 array distributions, and there are three electrode holes respectively for each receptacle unit, divide
Not with the working electrode line in microtrabeculae, reference electrode wire connects with to electrode wires, and circuit board internal circuit integration is by 8 × 12 arrays
Three-electrode system in the small-sized microtrabeculae of distribution, is integrated into circuit board two sides, realizes convenient and efficient formula high throughput detection；
Further, small-sized microtrabeculae is directly inserted into board in circuit board slot, and detection, which is completed directly to pull out, is inserted into new microtrabeculae, repeatedly
Circuit board recycling is realized in plug, and the receptacle unit on the small-sized microtrabeculae and integrated circuit board of several independents can also be set
Count into the high-throughput microtrabeculae electrochemical sensor of 16 × 16 arrays or 16 × 24 arrays；
Further, a kind of preparation method of the electrochemical sensor of microtrabeculae high throughput, it is described the preparation method is as follows:
Step 1: the plastic long tube for choosing suitable diameter according to demand is fixed vertically, by each of three electrodes silk edge with insulating layer
Plastic long tube upper end is put into；
Step 2: polymer and coagulator being sufficiently mixed and stir evenly removing bubble, by polymer and coagulated along plastic long tube upper end
Gu the mixture of agent pours into；
Step 3: plastic long tube is transferred in baking oven, to the polymer coagulation forming in pipe, scratches plastic long tube simultaneously with scissors
It removes, obtains the longer polymer microtrabeculae with three-electrode system；
Step 4: the microtrabeculae of height required for longer polymer microtrabeculae is cut into removes small-sized microtrabeculae one end appropriate poly-
It closes the wire electrode that object leaks out part and thus obtains the small-sized microtrabeculae electrochemical sensor of batch convenient for connecting with integrated circuit board；
Further, the diameter range of the plastic long tube is 5 millimeters to 5 centimetres, and plastic long tube is triangular prism, cylinder, rectangular
Body or hexagonal prisms, the small-sized microtrabeculae sensor select polymerization of different nature according to the temperature and detection acid or alkali environment that use
Object, the polymer include PDMS, PMMA and PLA, the flexibility and adhesion property of the sensor by regulation polymer with
The proportion of coagulator manipulates；
Further, the sensor can be realized by aptamers combination, the method for cDNA chip and protein adsorption to grape
Sugar, pathogenic bacteria, albumen, the high-throughput detection of nucleic acids marker, the small-sized microtrabeculae can also cover type demoulding and milling by 3D printing
Bed processing method preparation；
Beneficial effects of the present invention are as follows:
1, microtrabeculae high-flux electric chemical sensor flexible design of the present invention, at low cost, functional modification, the detection scale of construction are small,
Convenient for high throughput integration；
2, three-electrode system of the present invention can realize that Conventional electrochemical detects, simultaneously because noble metal electrode stability is good, this
Kind small electrical chemical sensor can be used repeatedly；
3, small-sized microtrabeculae flexible design of the present invention, it is at low cost, can batch making.
Detailed description of the invention
Fig. 1 show small-sized microtrabeculae electrochemical sensor sectional view；
Fig. 2 show single small-sized microtrabeculae electrochemical sensor shearogram；
Fig. 3 show different shape microtrabeculae electrochemical sensor schematic diagram；
Fig. 4 show single microtrabeculae and integrated circuit board integrates schematic diagram；
Fig. 5 show the electrochemical sensor schematic diagram of 96 microtrabeculaes high throughput；
Fig. 6 show the fixed microlayer model array schematic diagram of electrochemical sensor of 96 microtrabeculaes high throughput；
Fig. 7 show the electrochemical sensor high throughput detection ferrocene datagram of 96 microtrabeculaes high throughput；
Fig. 8 show gold electrode and dendritic gold electrode performance comparison figure；
Fig. 9 show microtrabeculae electrochemical sensor detection Tau protein data figure.
Specific embodiment
Below in conjunction with specific attached drawing the present invention is described in detail specific embodiment.It should be noted that in following embodiments
The combination of the technical characteristic or technical characteristic of description is not construed as isolated, they can be combined with each other to reaching
To superior technique effect.In the drawings of the following embodiments, the identical label that each attached drawing occurs represent identical feature or
Person's component, can be apply to different embodiments.
To keep the technical problem to be solved in the present invention, technical solution and advantage clearer, below in conjunction with attached drawing and tool
Body embodiment is described in detail.
As shown in figs. 1-9, the present invention provides a kind of electrochemical sensor of microtrabeculae high throughput, and the sensor includes micro-
Drop fixed test unit and electric signal transmission unit.The microlayer model fixed test unit is the small of insertion three-electrode system
Type polymer microtrabeculae, surface adhesion can fix micro drop.The electric signal transmission unit is integrated circuit board, circuit
Slot on plate is connect with microtrabeculae end electrode wires outstanding, and independent microtrabeculae is integrated into identical platform, micro- liquid is realized
The high-flux electric chemical detection of drop.
The sensor is to be directly connected to preparation by integrated circuit board and multiple small-sized microtrabeculaes, and middle-size and small-size microtrabeculae can
Large scale preparation is realized by simple casting method.
The plastic long tube for being suitble to size is chosen first, and three plain conductors with insulating layer are put into from long tube one end
Blend compounds band seals one end of long tube.It is vertical to place long tube and metal wire is straightened, polymer pre-polymerization is poured into the non-sealing end of long tube
Object and being transferred to baking oven solidifies polymer.Object coagulation forming to be polymerized, scratches plastic long tube with scissors, and obtaining the inside has three
The polymer microtrabeculae unified with long length of tube of root electrode wires, cuts polymer microtrabeculae further according to Miniature Sensor length requirement
At millimeter or centimetre length, and this small-sized microtrabeculae one end removed into appropriate polymer leaks out partial electrode silk and be convenient for and integrated electricity
Road plate is connected.
The acid or alkali environment that the polymer of microtrabeculae can be used according to microtrabeculae, temperature height are prepared, solution polarity etc. carries out multiplicity
Change selection, such as PDMS, PMMA, PLA etc..Furthermore adherency of the micro-post surface to drop, microtrabeculae flexible rigidity etc. can pass through regulation
Polymer and coagulator ratio are realized.The shape of microtrabeculae can select triangular prism, cylinder, cuboid, hexagonal prisms etc. by demand
Different shape plastic long tube.
Three-electrode system in microtrabeculae chooses precious metal wire such as gold, and silver, platinum etc., generally silver are used as reference electrode, platinum conduct
To electrode, Jin Zuowei working electrode.Package Teflon insulating layer avoids three metals in microtrabeculae preparation process outside metal wire
Line contact leads to short circuit.
The sensor can meet Conventional electrochemical detection, to improve this sensor to the detection of particular matter and sensitive
The promotion of degree can carry out multi-functional modification on the working electrode in the three-electrode system of array micro-post surface, such as modify
Dendritic gold, enzyme, carbon nanotube etc. carry out highly sensitive high accuracy Electrochemical Detection.
Trace detection liquid can be fixed by the adhesion strength of micro-post surface and realize electric signal in drop by the sensor
Transmission, can be realized by the functional modification of working electrode to glucose, pathogenic bacteria, albumen, the high throughput of nucleic acids marker etc.
High accuracy detection.
The sensor can be connected by small-sized microtrabeculae bottom three electrode wires outstanding with integrated circuit board slot mouth
, this connection is simple and fast, while can directly remove after having used a microtrabeculae and change another microtrabeculae into, realizes integrated electricity
The repeatability of road plate utilizes.
12 × 8 array manner in the sensor, the one of the only this integrated high-flux electric chemical sensor of microtrabeculae
A example, it can also be designed to 256,384 or the high-throughput microtrabeculae electrochemical sensors of General Cell.Furthermore the preparation of microtrabeculae
It is not limited only to simple casting method, can also cover the methods of type demoulding and milling machine processing preparation by 3D printing.
It is explained combined with specific embodiments below.
Embodiment 1
A kind of electrochemical sensor of microtrabeculae high throughput includes integrated circuit board and small-sized microtrabeculae electrochemical sensing unit, medium and small
Type microtrabeculae can industrialized production, the present embodiment introduces the preparation method of small-sized microtrabeculae using PDMS as microtrabeculae substrate, comprising:
Step 1: the plastic long tube of both ends open is placed vertically, wraps up the gold of Teflon insulating layer, and silver-colored, platinum line is long along plastics
Tube top end is put into, uses adhesive tape to fix each of three electrodes line in long tube bottom end and to seal bottom end standby as the container for preparing microtrabeculae sensor
With.
Step 2: PDMS prepolymer and coagulator are uniformly mixed with the ratio of 10:1, gas is drained in vacuum oven
It is filled after bubble along long tube top, 4-5 hours in the environment of long tube the is placed on 80 DEG C PDMS microtrabeculaes solidified, shown in Fig. 1
For microtrabeculae sectional view, outermost layer is plastic long tube, and centre is microtrabeculae polymer, is wrapped up in polymer with Teflon insulating layer
Three electrode wires.
Step 3: plastic long tube is scratched into removing PDMS microtrabeculae with knife, the strip microtrabeculae with three electrode wires is obtained, will grow
Microtrabeculae cuts batches of small size microtrabeculae.
Step 4: obtained small electrical chemistry microtrabeculae bottom end is cut out remove part PDMS as shown in Figure 2, so that one end leakage portion
Sub-electrode line peels off the insulating layer on electrode wires surface, and electrode wires, which can be used to connect with integrated circuit board, realizes high-flux electric chemistry
Sensing, thus small-sized microtrabeculae electrochemical sensor preparation is completed.In addition, small-sized microtrabeculae electrochemical sensor can pass through Demand Design
At different shape, it is illustrated in figure 3 cylinder, triangular prism, cuboid and hexagonal prisms decline column electrochemical sensor.
Embodiment 2
12 × 8(96 of array) microtrabeculae high throughput electrochemical sensor combination
The integrated circuit board with 8 × 12 array receptacle units, each receptacle unit are designed by circuit design software
There are three sockets for tool respectively with the working electrode in microtrabeculae, connects to electrode with reference electrode.96 are sensed inside circuit board
Working electrode is integrated into circuit board ends and facilitates high-throughput inspection by being integrated together respectively in unit to electrode and reference electrode
It surveys.
The small-sized microtrabeculae that embodiment 1 is obtained is connected (Fig. 4) according to slot mouth with circuit board, and integration obtains as shown in Figure 5
High-flux electric chemical sensor with 96 microtrabeculaes.
Embodiment 3
12 × 8(96 of array) microtrabeculae high throughput electrochemical sensor chemical property analysis: as embodiment 2 prepare array 12
× 8(96) electrochemical sensor of microtrabeculae high throughput is by gold electrode as working electrode, and silver electrode is as reference electrode, platinum electrode
Electrochemical sensing is carried out as to electrode.Microtrabeculae, which is respectively washed, with ethyl alcohol acetone and ultrapure water removes electrode surface impurity, array
12 × 8(96) microtrabeculae high throughput electrochemical sensor be added dropwise various concentration ferrocene phosphate buffer solution as electrolyte
(Fig. 6), wherein the concentration of ferrocene is respectively 1,2,3,4,5,6,7,8,9,10,15,20 mM, each measurement of concetration
Eight groups of parallel laboratory tests carry out cyclic voltammetry measurement under -0.6-0.3 V voltage, the symmetrical redox volt-ampere being closed
Curve is as shown in fig. 7, each concentration obtains complete cyclic voltammetry curve and gradually increases as concentration increases electric signal, explanation
The sensor has excellent chemical property, can be utilized for Conventional electrochemical detection.
Embodiment 4
12 × 8(96 of array) electrochemical sensor of microtrabeculae high throughput can carry out Conventional electrochemical detection, and it is more to improve its detection
Sample and sensitivity can carry out functional modification in electrode surface, and the present embodiment is introduced by taking the dendritic gold of working electrode deposition as an example
The functional modification of sensor.
12 × 8(96 of array) microtrabeculae high throughput the dendritic gold modification of the middle-size and small-size microtrabeculae working electrode of electrochemical sensor it is specific
Steps are as follows: by the electrode ethyl alcohol of micro-post surface, acetone and ultrapure water cleaning remove surface impurity, with 10 μ L 1mg/mL's
Chlorauric acid solution is deposition liquid, and 1000 s of electro-deposition is carried out under -1.5 V voltages, can grow branch on metal working electrode surface
The nanogold of shape structure.
To prove that dendritic gold enhances the sensitivity of microtrabeculae electrochemical sensor, by dendritic gold and common gold electrode electric signal
It is slow that phosphate of the 10 μ L comprising 0.1 M NaCl and 5 mM hexaammine ruthenium chlorides is added dropwise in comparison respectively on two kinds of electrode microtrabeculaes
Solution is rushed, differential pulse voltammetry measurement is carried out under -0.6-0.2 V voltage, there are two quantity for obtained dendritic golden electric signal
The increase (Fig. 8) of grade illustrates that dendritic gold modification significantly improves microtrabeculae sensor sensitivity.
Embodiment 5
12 × 8(96 of array) microtrabeculae high throughput electrochemical sensor biological marker analyte detection: by it is dendritic gold modification it is small
Type electrochemical sensor not only increases electrochemistry sensitivity, and coarse dendritic gold is aptamers, and enzyme and antibody provide more
Adsorption site, the present embodiment illustrates microtrabeculae electrochemical sensor in biological marker analyte detection by taking Tau Protein Detection as an example
Using.Testing principle is to be fixed Tau antibody on the working electrode (s using the absorption property of albumen and dendritic gold, is sealed using BSA
Tau antigen is added after stifled, electrode resistance significantly increases after antibody specificity crawl antigen, and electric signal gradually weakens, and passes through telecommunications
Number weaken size quantitative detection Tau concentration.
Specific implementation step is as follows: the microtrabeculae sensor for the dendritic golden functionalization that embodiment 3 obtains being cleaned up, by 10
The Tau protein antibodies that μ L concentration is 10 μ g/mL are added drop-wise to dendritic metal working electrode surface and react two hours so that antibody with
Dendritic gold sufficiently combines, then the bovine serum albumin of 10 μ L 1mg/ml is added drop-wise to micro-post surface reaction and is blocked within 2-3 hours,
Guarantee dendritic gold can not adhesion protein again, remove unbonded antibody and bovine serum albumin with the dendritic gold surface of ultrapure water.
After antibody modification, micro-post surface be added dropwise respectively various concentration Tau albumen (10 pg/mL, 100 pg/mL, 1
Ng/mL, 10ng/mL, 100 ng/mL), it is reacted 2-3 hours at 37 DEG C and makes the abundant binding protein of antibody, use is ultrapure
Water rinses microtrabeculae and removes unbonded albumen, slow in phosphate of the 10 μ L comprising 0.1M NaCl and 5 mM hexaammine ruthenium chlorides
It rushes solution and carries out differential pulse voltammetry detection, as shown in figure 9, working electrode resistance is gradually increased as protein concentration increases, electricity
Signal is gradually reduced, and is combined from there through antigen-antibody and is successfully realized the electrochemistry Protein Detection on microtrabeculae sensor.
Embodiment described above, only one kind of the present invention more preferably specific embodiment, those skilled in the art
The usual variations and alternatives that member carries out within the scope of technical solution of the present invention should be all included within the scope of the present invention.
Claims (10)
1. a kind of electrochemical sensor of microtrabeculae high throughput, which is characterized in that the sensor includes microlayer model fixed test list
Member and electric signal transmission unit, the microlayer model fixed test unit is for carrying out electrical signal detection and fixed micro drop, institute
The small-sized microtrabeculae that microlayer model fixed test unit is several independents is stated, the electric signal transmission unit is integrated circuit board, institute
State the small-sized microtrabeculae connection integrated circuit board of several independents.
2. sensor as described in claim 1, which is characterized in that the integrated circuit board by itself electrode hole with it is small-sized
The electrode wires plug-in connection outstanding of microtrabeculae end, the small-sized microtrabeculae of independent is integrated together, realizes the height in microlayer model
Flux Electrochemical Detection.
3. sensor as described in claim 1, which is characterized in that include three electrode bodies inside each small-sized microtrabeculae
System, electrical signal detection are carried out by the three-electrode system, and the small-sized micro-post surface adhesion strength can fix micro drop.
4. sensor as claimed in claim 3, which is characterized in that the three-electrode system is using Au-Ag-Pt metal wire as three
Electrode system, silver are used as reference electrode, and platinum is used as to electrode, Jin Zuowei working electrode, in the three-electrode system outside metal wire
Layer all has Teflon thin layer, avoids contacting with each other between three kinds of metal wires and causes short circuit.
5. sensor as claimed in claim 4, which is characterized in that the three-electrode system has multi-functional modification, described more
Functionalized modification includes the dendritic gold of gold surface modification nanoscale, graphene absorption and carbon nanotube deposition.
6. sensor as claimed in claim 4, which is characterized in that the small-sized microtrabeculae of several independents is 8 × 12 arrays
Distribution, the integrated circuit board have 8 × 12 array socket units, respectively correspond the small-sized microtrabeculae of 8 × 12 array distributions, each
Receptacle unit respectively there are three electrode hole, respectively in small-sized microtrabeculae working electrode line, reference electrode wire and electrode wires are connected
It connects, integrated circuit board internal circuit is integrated the three-electrode system in the small-sized microtrabeculae of 8 × 12 array distributions, and integrated electricity is integrated into
Convenient and efficient formula high throughput detection is realized in road plate two sides.
7. sensor as claimed in claim 6, which is characterized in that small-sized microtrabeculae is directly inserted into the slot list of integrated circuit board
Member, detection are completed directly pull out the new small-sized microtrabeculae of insertion, and repeatedly integrated circuit board recycling is realized in plug, described several
It can also be designed to 16 × 16 arrays or 16 × 24 arrays for receptacle unit on the small-sized microtrabeculae and integrated circuit board of independent
High-throughput microtrabeculae electrochemical sensor.
8. a kind of preparation method of the electrochemical sensor of microtrabeculae high throughput, based on biography described in one of the claims 1-7
Sensor, which is characterized in that it is described the preparation method is as follows:
Step 1: the plastic long tube for choosing suitable diameter according to demand is fixed vertically, by each of three electrodes silk edge with insulating layer
Plastic long tube upper end is put into；
Step 2: polymer and coagulator being sufficiently mixed and stir evenly removing bubble, by polymer and coagulated along plastic long tube upper end
Gu the mixture of agent pours into；
Step 3: plastic long tube is transferred in baking oven, to the polymer coagulation forming in pipe, scratches plastic long tube simultaneously with scissors
It removes, obtains the longer polymer microtrabeculae with three-electrode system；
Step 4: the small-sized microtrabeculae of height required for longer polymer microtrabeculae is cut into removes small-sized microtrabeculae one end suitable
When thus the wire electrode of polymer leakage part obtains the electrification of the microtrabeculae high throughput of batch convenient for connecting with integrated circuit board
Learn sensor.
9. preparation method as claimed in claim 8, which is characterized in that the diameter range of the plastic long tube is 5 millimeters to 5 lis
Rice, plastic long tube are triangular prism, cylinder, cuboid or hexagonal prisms, and the small-sized microtrabeculae sensor is according to the temperature and inspection used
Survey acid or alkali environment and select polymer of different nature, the polymer includes PDMS, PMMA and PLA, the sensor it is soft
Property and adhesion property manipulated by the proportion of regulation polymer and coagulator.
10. preparation method as claimed in claim 8, which is characterized in that the electrochemical sensor of the microtrabeculae high throughput can pass through
Aptamers combine, the method for cDNA chip and protein adsorption, realize to glucose, pathogenic bacteria, albumen, the high pass of nucleic acids marker
Amount detects, and the small-sized microtrabeculae in the electrochemical sensor of the microtrabeculae high throughput can also cover type demoulding and milling machine by 3D printing
Processing method preparation.
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| CN112649366A (en) * | 2020-12-07 | 2021-04-13 | 北京科技大学 | Freely-assembled spliced micro-droplet detection chip capable of realizing multi-mode output |
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| CN113737235A (en) * | 2021-08-23 | 2021-12-03 | 西安交通大学 | Microelectrode structure preparation device and method based on electrofluid reduction drop printing |
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