CN202808804U - Humidifying temperature-controlling oscillatory type hybridization device - Google Patents
Humidifying temperature-controlling oscillatory type hybridization device Download PDFInfo
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- CN202808804U CN202808804U CN 201220553243 CN201220553243U CN202808804U CN 202808804 U CN202808804 U CN 202808804U CN 201220553243 CN201220553243 CN 201220553243 CN 201220553243 U CN201220553243 U CN 201220553243U CN 202808804 U CN202808804 U CN 202808804U
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Abstract
The utility model relates to a humidifying temperature-controlling oscillatory type hybridization device. The hybridization device comprises a base, an upper cover matched with the base, a lower heating plate and an upper heating board which are arranged on the base and the upper cover respectively, wherein the upper cover and the base are closed to form a heating cavity. The hybridization device is characterized in that a functional frame is supported on the base, the bottom of the functional frame is connected with a vibration motor which is in signal connection with a controller, a humidifying unit is disposed in the heating cavity and is in signal connection with the controller, the upper heating plate and the lower heating plate are in signal connection with a temperature control unit which is in signal connection with the controller. The hybridization device has the advantages that the hybridization device can perform humidification continuously and adjust the humidity and generate oscillation according to requirements of experiments within a relatively long period through a humidification control unit, the temperature control unit and the vibration motor; and the hybridization efficiency and quality can be improved.
Description
Technical field
The utility model relates to a kind of hybrid device, relates in particular to a kind ofly can continue humidification and in time adjust humidity, can produce the humidification temperature control oscillatory type hybrid device that vibrates according to the experiment needs simultaneously according to the experiment needs in relatively long-time.
Background technology
In situ hybridization: a kind of technology that grows up on the basis of researching DNA molecule replicating principle.Its ultimate principle is two nucleotide single-chain fragments, under suitable condition, can cross hydrogen bonded, form the characteristics of the two key molecules of DNA-DNA, DNA-RNA or RNA-RNA, application is with markd DNA or RNA fragment as nucleic acid probe, (RNA or dna fragmentation are hybridized, and then the method such as available radioautograph is shown, observe existence and the location of purpose mRNA or DNA under light microscopic or Electronic Speculum with determined nucleic acid in tissue slice or the cell.This method has very high susceptibility and specificity, can further inquire into functional expression and the regulation mechanism thereof of cell from molecular level.Become the important means of current cytobiology, molecular biology research.
In the hybrid experiment, most important factor is the pH value of temperature, illumination, humidity and all ingredients in position.And temperature and humidity directly affects the hybridization efficiency of probe and target dna.
Be better to increase humidity, prior art adopts polymer composite, porous ceramics, paper or leather suction to be placed on the humidity that means in the heating chamber keep heating chamber usually.Because the water yield that the suction material absorbs when adopting above-mentioned means to carry out humidification is less, can not regulate humidity simultaneously; And, vibrating mechanism is not set yet, can not make the abundant mixing of experiment material, affect hybridization efficiency.
Summary of the invention
The purpose of this utility model is to solve prior art exists the problem such as above-mentioned and a kind of humidification temperature control oscillatory type hybrid device is provided, and can continue humidification and in time adjust humidity, can produce according to the experiment needs and vibrate simultaneously according to the experiment needs in relatively long-time.
Above-mentioned technical purpose of the present utility model mainly solves by the following technical programs: humidification temperature control oscillatory type hybrid device, comprise pedestal, the loam cake that cooperates with pedestal, be separately positioned on pedestal and on the lower hot-plate and the upper hot-plate that cover, described loam cake and pedestal cover the formation heating chamber, it is characterized in that bearing one functional frame on the described pedestal, described functional frame bottom connects a vibrating motor, the controller that is connected with the vibrating motor signal, be provided with damping device in the described heating chamber, described damping device is connected with controller signals, and described lower hot-plate is connected with a temperature-control device signal with upper hot-plate, and described temperature-control device signal connects and controller.Effect by humidity conditioner, temperature-control device and vibrating motor, hybrid device is reached in relatively long-time, can continue humidification and in time adjust humidity, can produce the purpose of vibration according to the experiment needs, raising hybridization efficiency and quality simultaneously according to the experiment needs.In order conveniently to watch the situation in the heating chamber, loam cake, upper hot-plate (and upper heating film) adopt transparent material.
As further improving and replenishing technique scheme, the utility model adopts following technical measures: described damping device comprises water tank, be arranged on the well heater on the water tank, detect the humidity sensor of described heating chamber humidity, described humidity sensor all is connected with controller signals with well heater; Offer the humidity measurement hole on the described upper hot-plate, described humidity sensor is arranged in the humidity measurement hole.By the temperature sensor detected temperatures, feed back signal to controller, controller receives signal and control heater, in time is adjusted to appropriate temperature.
Described temperature-control device comprises the temperature sensor that detects described heating chamber temperature, the temperature sensor and the fan that are connected with described controller signals.When temperature sensor detects temperature in the heating chamber when inadequate, start heater heats, the temperature height in detecting heating chamber, then start fan and lower the temperature.
Heating film and lower heating film are posted respectively in the bottom surface of described lower hot-plate and upper hot-plate, and described temperature sensor cooperates with upper heating film, lower heating film and lower hot-plate end face respectively.In order to be homogeneous heating and the life-span of improving hot-plate, post respectively heating film.
Described loam cake periphery is provided with wear ring, also is provided with the touch display operation screen and the expansion interface that all are connected with controller signals on the described pedestal.Making heating chamber by wear ring is annular seal space.By touching handled easily is set and checks of display operation screen, carry out the expansion of function by expansion interface.
In order to make terminal with compact integral structure integrated, described pedestal has electric container cavity; The position that described pedestal cooperates with functional frame is the sunk type step, and described functional frame periphery has the limit of accepting, and the described limit of accepting is shelved on the sunk type step, and described motor and controller are arranged in the electric container cavity.The fit system of pedestal and functional frame makes things convenient for functional frame to pick and place, and when experiment, avoids the functional frame transverse shift, improves work reliability.
In order to make slide glass or hybridizing box be distributed in the region intermediate of heating chamber, temperature, humidity are suited, then described lower hot-plate is arranged on described functional frame region intermediate, in order to make processing, easy to operate, described water tank and described functional frame are structure as a whole and are arranged on and accept between limit and the lower hot-plate.
The end face of described lower hot-plate is provided with experiment carrier anchor, and described experiment carrier anchor is strip, and is respectively equipped with the evagination card division that a plurality of intervals arrange in the both sides of strip.The concrete structure of experiment carrier anchor greatly facilitates clamping slide glass or hybridizing box, has simplified operation sequence, and in experimentation, has avoided the generation of vibration displacement, has further improved the reliability that experiment is carried out.
Described experiment carrier anchor is provided with the temperature test hole that a plurality of intervals arrange, and corresponding described temperature sensor is arranged in the temperature test hole.Avoid temperature sensor exposed outside, avoid occurring being touched by accident and cause the phenomenon of cisco unity malfunction.
The complex root support post is set respectively between the bottom periphery of described functional frame and the pedestal, and described support post comprises upper contiguous block that the interval arranges with lower connecting block and connects upper contiguous block, the soft pipe connecting of lower connecting block.Support post has on the one hand supports the equilibrium function frame, avoids, and on the other hand, is used for balance vibration, avoids undue oscillation or undervibration.
The beneficial effect that the utlity model has: 1, pass through the effect of humidity conditioner, temperature-control device and vibrating motor, hybrid device is reached in relatively long-time, can continue humidification and in time adjust humidity, can produce the purpose of vibration according to the experiment needs, raising hybridization efficiency and quality simultaneously according to the experiment needs.And in order conveniently to watch the situation in the heating chamber, loam cake, upper hot-plate (and upper heating film) adopt transparent material usually.2, by touching handled easily is set and checks of display operation screen, carry out the expansion of function by expansion interface.3, the fit system of pedestal and functional frame makes things convenient for functional frame to pick and place, and when experiment, avoids the functional frame transverse shift, improves work reliability.4, the concrete structure of experiment carrier anchor greatly facilitates clamping slide glass or hybridizing box, has simplified operation sequence, and in experimentation, has avoided the generation of vibration displacement, has further improved the reliability that experiment is carried out.5, support post has support equilibrium function frame on the one hand, avoids, and on the other hand, is used for balance vibration, avoids undue oscillation or undervibration.
Description of drawings
Fig. 1 is the structural representation of a kind of state of uncapping of the present utility model.
Fig. 2 is the sectional structure synoptic diagram of a kind of closing lid state of the present utility model.
Embodiment
Below by embodiment, and by reference to the accompanying drawings, the technical solution of the utility model is described in further detail.
Embodiment: humidification temperature control oscillatory type hybrid device, as depicted in figs. 1 and 2, it comprises pedestal 1, the loam cake 2 that cooperates with pedestal 1, be separately positioned on lower hot-plate 3 and upper hot-plate 4 on pedestal 1 and the loam cake 2, loam cake 2 covers with pedestal 1 and forms heating chamber 5, and loam cake 2 peripheries are provided with wear ring 14, when loam cake covers on the pedestal, make heating chamber 5 form annular seal space.
Innovative point of the present utility model is: bearing one functional frame 6 on the pedestal 1, functional frame 6 bottoms connect a vibrating motor 7(and are made of motor and eccentric), the controller 11 that is connected with vibrating motor 7 signals, be provided with damping device in the described heating chamber 5, described damping device is connected with controller 11 signals, described lower hot-plate 3 is connected with a temperature-control device signal with upper hot-plate 4, and described temperature-control device signal connects and controller 11.
For functional frame 6 working stabilities are carried out, complex root support post 23 is set respectively between the bottom periphery of functional frame 6 and the pedestal, and support post 23 comprises that upper contiguous block 230 that the interval arranges is connected with lower connecting block and connects contiguous block 230, lower connecting block 231 soft pipe connectings 232.Support post 23 is used for absorbing, avoids functional frame 6 fierce vibrations.
A kind of concrete mode that pedestal and functional frame cooperate: the position that pedestal 1 cooperates with functional frame 6 is sunk type step 18, and functional frame 6 peripheries have the limit of accepting 19, accepts limit 19 and is shelved on the sunk type step 18.This fit system is conducive to be convenient for changing the functional frame 6 of taking.Certainly, sunk type step 18 is not set on the pedestal 1, on the functional frame 6 to accept that limit 19 directly is shelved on also be feasible on the pedestal 1.
Specify the present patent application scheme: damping device comprises water tank 8, is arranged on the well heater 9 on the water tank 8, detects the humidity sensor 10 of heating chamber 5 humidity, and humidity sensor 10 is connected with well heater and all is connected with controller 11 signals; Offer humidity measurement hole 101 on the upper hot-plate 4, humidity sensor 10 is arranged in the humidity measurement hole 101.
Temperature-control device comprises the temperature sensor 12 that detects heating chamber 5 temperature, the temperature sensor 12 and the fan 13 that are connected with controller 9 signals.
The end face of lower hot-plate 3 is provided with experiment carrier anchor 20, and experiment carrier anchor 20 is strip, and is respectively equipped with the evagination card division 21 that a plurality of intervals arrange in the both sides of strip.Experiment carrier anchor 20 is provided with the temperature test hole 22 that a plurality of intervals arrange, and corresponding temperature sensor 22 is arranged in the temperature test hole 22, and namely the temperature sensor 22 on the lower heating film 31 is arranged in the temperature test hole 22.
During experiment, some slide glasss 24 are stuck between the adjacent evagination card division 21 or with some hybridizing boxes 25 are fixed between the corresponding evagination card division 21, in order to make hybridizing box 25 stable being stuck on the experiment carrier anchor 20, also be provided with interior cancave embedded part 251 on the hybridizing box 25, cancave embedded part 251 in insert corresponding evagination card division 21, evagination card division 21 by two ends holds and the interior cancave embedded part 251 by the middle part cooperates the purpose that reaches stable holding hybridizing box 25 with evagination card division 21 inlay cards, and experiment is carried out smoothly.
For the expansion of handled easily and function, also be provided with the certain button display operation screen of touch display operation screen 15(that all is connected with controller 11 signals on the pedestal 1 and also be suitable for) and expansion interface 16.
In order to make the one-piece construction outward appearance succinct, have electric container cavity 17 on the pedestal 1, motor 7 and controller 11 are arranged in the electric container cavity 17.
The above is preferred embodiment of the present utility model only, is not limited to the utility model.In the above-described embodiments, the utility model can have various modifications and variations.All within spirit of the present utility model and principle, any modification of doing, be equal to replacement, improvement etc., all should be included within the protection domain of the present utility model.
Claims (10)
1. humidification temperature control oscillatory type hybrid device, comprise pedestal (1), the loam cake (2) that cooperates with pedestal (1), be separately positioned on lower hot-plate (3) and upper hot-plate (4) on pedestal (1) and the loam cake (2), described loam cake (2) covers with pedestal (1) and forms heating chamber (5), it is characterized in that upper bearing one functional frame (6) of described pedestal (1), described functional frame (6) bottom connects a vibrating motor (7), the controller (11) that is connected with vibrating motor (7) signal, described heating chamber is provided with damping device in (5), described damping device is connected with controller (11) signal, described lower hot-plate (3) is connected with a temperature-control device signal with upper hot-plate (4), and described temperature-control device signal connects and controller (11).
2. humidification temperature control oscillatory type hybrid device according to claim 1, it is characterized in that described damping device comprises water tank (8), be arranged on the well heater (9) on the water tank (8), detect the humidity sensor (10) of described heating chamber (5) humidity, described humidity sensor (10) is connected 9 with well heater) all be connected with controller (11) signal; Offer humidity measurement hole (101) on the described upper hot-plate (4), described humidity sensor (10) is arranged in the humidity measurement hole (101).
3. humidification temperature control oscillatory type hybrid device according to claim 2, it is characterized in that described temperature-control device comprises the temperature sensor (12) that detects described heating chamber (5) temperature, the temperature sensor (12) and the fan (13) that are connected with described controller (9) signal.
4. humidification temperature control oscillatory type hybrid device according to claim 3, heating film (41) and lower heating film (31) are posted respectively in the bottom surface that it is characterized in that described lower hot-plate (3) and upper hot-plate (4), and described temperature sensor (12) cooperates with the end face of upper heating film (41), lower heating film (31) and lower hot-plate (3) respectively.
5. optional described humidification temperature control oscillatory type hybrid device according to claim 2-4, it is characterized in that described loam cake (2) periphery is provided with wear ring (14), also be provided with the touch display operation screen (15) and the expansion interface (16) that all are connected with controller (11) signal on the described pedestal (1).
6. optional described humidification temperature control oscillatory type hybrid device according to claim 1-4, it is characterized in that described pedestal (1) has electric container cavity (17), the position that described pedestal (1) cooperates with functional frame (6) is sunk type step (18), described functional frame (6) periphery has the limit of accepting (19), the described limit (19) of accepting is shelved on the sunk type step (18), and described motor (7) and controller (11) are arranged in the electric container cavity (17).
7. humidification temperature control oscillatory type hybrid device according to claim 6, it is characterized in that described lower hot-plate (3) is arranged on the region intermediate of described functional frame (6), described water tank (8) and described functional frame (6) are structure as a whole and are arranged on and accept between limit (19) and the lower hot-plate (3).
8. humidification temperature control oscillatory type hybrid device according to claim 7, the end face that it is characterized in that described lower hot-plate (3) is provided with experiment carrier anchor (20), described experiment carrier anchor (20) is strip, and is respectively equipped with the evagination card division (21) that a plurality of intervals arrange in the both sides of strip.
9. humidification temperature control oscillatory type hybrid device according to claim 8, it is characterized in that described experiment carrier anchor (20) is provided with the temperature test hole (22) that a plurality of intervals arrange, corresponding described temperature sensor (22) is arranged in the temperature test hole (22).
10. optional described humidification temperature control oscillatory type hybrid device according to claim 1-4, it is characterized in that between the bottom periphery of described functional frame (6) and the pedestal complex root support post (23) being set respectively, described support post (23) comprises that the upper contiguous block (230) that the interval arranges is connected 231 with lower connecting block) and connect and go up contiguous block (230), the soft pipe connecting of lower connecting block (231) (232).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220553243 CN202808804U (en) | 2012-10-25 | 2012-10-25 | Humidifying temperature-controlling oscillatory type hybridization device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220553243 CN202808804U (en) | 2012-10-25 | 2012-10-25 | Humidifying temperature-controlling oscillatory type hybridization device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202808804U true CN202808804U (en) | 2013-03-20 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201220553243 Withdrawn - After Issue CN202808804U (en) | 2012-10-25 | 2012-10-25 | Humidifying temperature-controlling oscillatory type hybridization device |
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| Country | Link |
|---|---|
| CN (1) | CN202808804U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102899248A (en) * | 2012-10-25 | 2013-01-30 | 杭州奥盛仪器有限公司 | Humidification temperature-controlled oscillation type hybridization device |
| CN103992945A (en) * | 2014-05-19 | 2014-08-20 | 东南大学 | Constant-temperature incubation and hybridization device |
-
2012
- 2012-10-25 CN CN 201220553243 patent/CN202808804U/en not_active Withdrawn - After Issue
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102899248A (en) * | 2012-10-25 | 2013-01-30 | 杭州奥盛仪器有限公司 | Humidification temperature-controlled oscillation type hybridization device |
| CN102899248B (en) * | 2012-10-25 | 2014-11-26 | 杭州奥盛仪器有限公司 | Humidification temperature-controlled oscillation type hybridization device |
| CN103992945A (en) * | 2014-05-19 | 2014-08-20 | 东南大学 | Constant-temperature incubation and hybridization device |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| AV01 | Patent right actively abandoned |
Granted publication date: 20130320 Effective date of abandoning: 20141126 |
|
| RGAV | Abandon patent right to avoid regrant |