CN201695044U - Medicine filling and perfusion device for closed culture chamber - Google Patents
Medicine filling and perfusion device for closed culture chamber Download PDFInfo
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- CN201695044U CN201695044U CN2010201965517U CN201020196551U CN201695044U CN 201695044 U CN201695044 U CN 201695044U CN 2010201965517 U CN2010201965517 U CN 2010201965517U CN 201020196551 U CN201020196551 U CN 201020196551U CN 201695044 U CN201695044 U CN 201695044U
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Abstract
The utility model belongs to the technical field of microculture machinery, relates to a medicine filling and perfusion device for a closed culture chamber, which comprises a circular support of a culture chamber, a circular support of a culture dish, a thin hose, a thin steel tube, an upper cover of the culture chamber, an upper cover of the culture dish, a base of the culture chamber, a base of the culture dish, an injection syringe and a perforated steel tube, wherein the circular support is installed between the upper cover of the culture chamber and the base, a hole is drilled in the wall, and the thin hose is penetrated through the hole; the circular support is installed between the upper cover of the culture dish and the bottom of the dish, the hole is drilled in the wall of the culture dish wall, and the thin steel tube is penetrated through the hole. The device can be used on the closed culture chamber matched with a living cell work station and a disposable glass bottom culture dish. Under the premise of not changing the inner environment of the culture chamber, reagent medicine addition or metabolite or culture medium changing can be carried out on the cells, chemical gradient can be prevented from generating, and the influence on the cell growth when the liquid culture medium is changed is reduced to the minimum extent.
Description
Technical field
The utility model belongs to microorganism culturing technique with the apparatus field, is specifically related to a kind of dosing perfusion device that is used for closed cultivation cell.This dosing perfusion device can be engaged at the bottom of supporting closed cultivation cell of viable cell workstation and the disposable glass and use on the culture dish.
Background technology
At present, life science begins to pay close attention to more and more the dynamic growth process of cell.This imaging that just need utilize the viable cell workstation to carry out the long time-histories of viable cell is observed, and wherein, culturing room is a very important basic link in the viable cell imaging research.At present, the cultivation cell that is installed on the microscope stage has replaced the box-type culture systems gradually, becomes the main flow culture apparatus of viable cell workstation.
At present, multiple cultivation cell is developed to satisfy the demand of different researchs, but there is following various defective, as: the compound cultivation cell that has multiple function is very expensive, and be installed in and be used for accurately controlling the localized piezoelectric ceramic device of Z axle on the Stage microscope size of cultivating cell is limited to some extent, therefore, the function and the price of cell cultivated in balance, and domestic researcher is the most normal, and what select for use is to cooperate the small enclosed that culture dish uses at the bottom of the disposable glass to cultivate cell.Described closed cultivation cell is embedded on the Stage microscope that is installed in inverted microscope in the piezoelectric ceramic device, is made of the structure of airtight chamber top cover and base two portions.During use, place disposable glass floor cells culture dish therebetween, and remain on the level that is fit to the cell growth by special controller adjusting room temp, CO2 concentration.Above-mentioned closed cultivation cell can satisfy basic experiment demand.But, when the experimenter need operate culture sample in experimentation, adding (medicine or meta-bolites) or change substratum etc. as reagent, the experimenter need open the cell top cover lid of opening culture dish again, and very inconvenience seems.Aforesaid operations not only can change pericellular environment, influences the process of growth of cell, also may change the position of original object observing at the removable cover period of the day from 11 p.m. to 1 a.m, causes the cell that can not find original observation, will greatly influence experimental result.
Summary of the invention
The purpose of this utility model is for overcoming the defective that prior art exists, a kind of dosing perfusion device that is used for closed cultivation cell being provided.This device just can be given the culturing cell dosing without uncap.
Particularly, the dosing perfusion device that is used for closed cultivation cell of the present utility model, it is characterized in that, make by cultivating cell ring support (1), culture dish annular support (2), small hose (3), fine steel tube (4), cultivation cell loam cake (5), culture dish loam cake (6), cultivation cell base (7), culture dish base (8), syringe (9) and porous steel pipe (10) combination.
In the utility model, between the loam cake (5) of cultivation cell and the base (7) ring support (1) is installed, the edge up and down of ring support (1) agrees with loam cake (5), the base (7) of cultivating cell respectively, cultivates on the little locular wall and holes, run through inside and outside making cell, small hose (3) penetrates from the hole;
In the utility model, culture dish is a culture dish at the bottom of the disposable glass;
In the utility model, ring support (2) is installed between (8) at the bottom of culture dish loam cake (6) and the ware, edge agrees with culture dish loam cake (6), base (8) respectively about it, hole on the wall, make the inside and outside perforation of ware, penetrate fine steel tube (4), the ware outside part of steel pipe (4) links with introducing the small hose (3) of cultivating cell, part bending at the bottom of ware in the ware.
In the utility model, during perfusion, corresponding straight two fine steel tubes (4) are installed, end respectively connects a porous fine steel tube (10), during dosing, the small hose (3) outside cultivating cell medicine is injected, medicine enters culture dish at last by flexible pipe (3) and fine steel tube (4), finishes the interpolation of reagent.
The utility model is used for the experiment that needs are changed whole liquid nutrient mediums, and this situation not only needs to realize bigger perfusion flow (having into has), requires perfusion to change the liquid process simultaneously and finishes in mode as mild as a dove, in order to avoid influence the process of growth of cell.In order to satisfy this demand, in the utility model, on the diametric(al) of the supporting ring support of culture dish (2), bore two holes, fine steel tube (4) is installed in a hole, influx as liquid nutrient medium, the spout of fine steel tube (4) as liquid nutrient medium installed in another hole, the responsible inflow and the effusive fine steel tube (4) that stretch in the culture dish will be bent downwardly to the bottom near ware, simultaneously, the end of fine steel tube (4) will respectively connect the porous fine steel tube (10) of a horizontal positioned, the process of changing liquid nutrient medium is finished in the mode of advection, can be avoided the generation of chemical gradient, drop to the influence of changing liquid nutrient medium process cell growth minimum.
Characteristics of the present utility model are:
Need not open and cultivate cell and culture dish, can not change under the cultivation cell interior environmental conditions, cell in the culture dish in the cultivation cell be carried out reagent add (medicine or meta-bolites) or change substratum, and can not influence the experimental observation before and after the dosing.
For the ease of understanding, below will describe in detail dosing perfusion device of the present utility model by concrete drawings and Examples.It needs to be noted, specific examples and accompanying drawing only are in order to illustrate, obviously those of ordinary skill in the art can illustrate according to this paper, in scope of the present utility model the utility model is made various corrections and change, these corrections and change are also included in the scope of the present utility model.
Description of drawings
Fig. 1 is a dosing perfusion device structural representation of the present utility model.
Fig. 2 is the utility model dosing perfusion device administration state plan structure synoptic diagram.
Fig. 3 is the side-looking structural representation of dosing perfusion device administration state.
Fig. 4 is the plan structure synoptic diagram of dosing perfusion device perfusion state.
Fig. 5 is the side-looking structural representation of dosing perfusion device perfusion state.
Indicate among the figure: the 1st, cultivate the cell ring support, the 2nd, culture dish annular support, the 3rd, small hose, the 4th, fine steel tube, the 5th, cultivate the cell loam cake, the 6th, the culture dish loam cake, the 7th, cultivate the cell base, the 8th, culture dish base, the 9th, syringe, the 10th, porous steel pipe.
Embodiment
The structure of exemplary dosing perfusion device is shown in Fig. 1-5, it comprises cultivates cell ring support (1), culture dish annular support (2), small hose (3), fine steel tube (4), cultivation cell loam cake (5), culture dish loam cake (6), cultivation cell base (7), culture dish base (8), syringe (9) and porous steel pipe (10)
Cultivating cell ring support (1) is placed between the loam cake (5) and base (7) of closed cultivation cell, agree with cultivation cell loam cake (5) and base (7), guarantee to cultivate the relative resistance to air loss of cell, run through inside and outside cultivating cell punchinging on the position that this support is fit to make, penetrate small hose (3); Culture dish annular support (2) is placed between the loam cake (6) and base (8) of culture dish, agree with culture dish loam cake (6) and base (8), guarantee the relative resistance to air loss of culture dish, connect inside and outside making ware punchinging on the position that this support is fit to, penetrate fine steel tube (4) and fixing, steel pipe ware outside part links with penetrating the small hose (3) of cultivating cell, part bending at the bottom of ware in the ware.During dosing, connect syringe (9), syringe (9) Chinese traditional medicine is injected,, enter capsule at last, finish the interpolation of reagent by small hose (3) and fine steel tube (4) by cultivating the outer small hose (3) of cell.During perfusion, the fine steel tube of using when using two porous steel pipes (10) to change dosing (4) is cultivated outer two small hoses (3) of cell and is connected peristaltic pump respectively, realizes the replacing of nutrient solution in the ware.
Claims (8)
1. the dosing perfusion device that is used for closed cultivation cell, it is characterized in that, form by cultivating cell ring support (1), culture dish annular support (2), small hose (3), fine steel tube (4), cultivation cell loam cake (5), culture dish loam cake (6), cultivation cell base (7), culture dish base (8), syringe (9) and porous steel pipe (10); Between loam cake of described cultivation cell (5) and the base (7) ring support (1) is installed, is cultivated on the little locular wall and hole, small hose (3) penetrates from the hole; Ring support (2) is installed between (8) at the bottom of described culture dish loam cake (6) and the ware, is holed on the culture dish wall, penetrate fine steel tube (4).
2. by the described dosing perfusion device of claim 1, it is characterized in that the edge up and down that cell ring support (1) is cultivated in described boring agrees with loam cake (5), the base (7) of cultivating cell respectively.
3. by the described dosing perfusion device of claim 1, it is characterized in that, run through inside and outside the cultivation cell of described boring.
4. by the described dosing perfusion device of claim 1, it is characterized in that, described culture dish annular support (2), edge agrees with culture dish loam cake (6), base (8) respectively about it.
5. by the described dosing perfusion device of claim 1, it is characterized in that the culture dish of described boring is inside and outside to connect.
6. by the described dosing perfusion device of claim 1, it is characterized in that, part bending at the bottom of ware in described fine steel tube (4) ware, the small hose (3) of the ware outside part of fine steel tube (4) and the cultivation cell of introducing links.
7. by the described dosing perfusion device of claim 1, it is characterized in that, during dosing, connect syringe (9), syringe (9) Chinese traditional medicine is injected,, enter capsule at last by small hose (3) and fine steel tube (4) by cultivating the outer small hose (3) of cell.
8. by the described dosing perfusion device of claim 1, it is characterized in that during perfusion, the fine steel tube of using when adopting two porous steel pipes (10) to change dosing (4) is cultivated outer two small hoses (3) of cell and connected peristaltic pump respectively.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010201965517U CN201695044U (en) | 2010-05-17 | 2010-05-17 | Medicine filling and perfusion device for closed culture chamber |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010201965517U CN201695044U (en) | 2010-05-17 | 2010-05-17 | Medicine filling and perfusion device for closed culture chamber |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201695044U true CN201695044U (en) | 2011-01-05 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010201965517U Expired - Fee Related CN201695044U (en) | 2010-05-17 | 2010-05-17 | Medicine filling and perfusion device for closed culture chamber |
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| CN (1) | CN201695044U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104928180A (en) * | 2015-06-29 | 2015-09-23 | 重庆大学 | Culture dish device based on base of micro-column type topological structure and application method of culture dish device in target responsiveness reinforcement |
| CN106318866A (en) * | 2015-06-24 | 2017-01-11 | 中国科学院大连化学物理研究所 | Small multi-purpose cell culturing and testing device |
| CN110373323A (en) * | 2019-07-27 | 2019-10-25 | 万贤能 | A kind of Synthetical cultivation system and method |
-
2010
- 2010-05-17 CN CN2010201965517U patent/CN201695044U/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106318866A (en) * | 2015-06-24 | 2017-01-11 | 中国科学院大连化学物理研究所 | Small multi-purpose cell culturing and testing device |
| CN106318866B (en) * | 2015-06-24 | 2019-06-18 | 中国科学院大连化学物理研究所 | A kind of multipurpose small cell culture and experimental provision |
| CN104928180A (en) * | 2015-06-29 | 2015-09-23 | 重庆大学 | Culture dish device based on base of micro-column type topological structure and application method of culture dish device in target responsiveness reinforcement |
| CN110373323A (en) * | 2019-07-27 | 2019-10-25 | 万贤能 | A kind of Synthetical cultivation system and method |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20110105 Termination date: 20130517 |