CN110373323A - A kind of Synthetical cultivation system and method - Google Patents
A kind of Synthetical cultivation system and method Download PDFInfo
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- CN110373323A CN110373323A CN201910685527.5A CN201910685527A CN110373323A CN 110373323 A CN110373323 A CN 110373323A CN 201910685527 A CN201910685527 A CN 201910685527A CN 110373323 A CN110373323 A CN 110373323A
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Abstract
The present invention relates to field of cell culture, a kind of Synthetical cultivation system and method are provided, for solving the problems, such as that cell culture environment is unstable.A kind of Synthetical cultivation system provided by the invention, including closed case, microscope, cultivation region, display, mechanical arm, culture tampering devic, environment regulating device, the microscope, cultivation region, mechanical arm are arranged in closed case, the display is connected with microscope, and the cultivation region is arranged in microscopical observation area；Culture dish, quantity >=1 of the culture dish are provided in the cultivation region；The culture tampering devic includes the first tampering devic and the second tampering devic, and first tampering devic is arranged in closed case, and second tampering devic is connected with cultivation region.The stability and conventional efficient for improving the environment of cell culture experiments can be improved the precision of the experimental result of single-factor influence experiment, ensure that the accuracy of experimental data.
Description
Technical field
The present invention relates to field of cell culture, and in particular to a kind of Synthetical cultivation system and method.
Background technique
The growth of biology is many by environmental impact factor, and the bat of such as permanent life in the dark, eyes pass through the centuries
Or it slowly degenerates after thousands of years, but hearing organ is flourishing；And in present environment, all shadows such as electromagnetic wave, sound wave, temperature, humidity
Ring the growth of biology.
In Bioexperiment, colored direction can be changed with the movement of light source from simple sunflower, from moist class worm
Son like wet environment is medium and culture dish in variation etc. of the microorganism mushroom by external influence, current experiment
All be special equipment, poor universality, all kinds of instrument and equipments such as Bioexperiment unit such as school, R&D institution, medical institutions all
Purchase, invisible is a kind of no small expenditure.
It is stable that the closed culture apparatus of current major part, which all not can guarantee the indoor environment of experiment,.
Summary of the invention
Present invention solves the technical problem that providing a kind of Synthetical cultivation system to solve the problems, such as that cell culture environment is unstable
System and method.
In order to solve the above technical problem, the present invention provides technical solution are as follows:
A kind of Synthetical cultivation system, including closed case, microscope, cultivation region, mechanical arm, tampering devic, the microscope, training
Area, mechanical arm setting are supported in closed case, the cultivation region is arranged in microscopical observation area；It is provided in the cultivation region
Culture dish, microscopical quantity >=1, quantity >=1 of the culture dish；The tampering devic include the first tampering devic and
Second tampering devic, first tampering devic are connected with cultivation region, and second tampering devic is trained outside culture dish for adjusting
The environment in case is supported, first tampering devic passes through the exogenous conduit connection of at least one with cultivation region；The closed case
It is made of metal, plastics, the one of which of glass or different materials.
Culture environment is adjusted by environment regulating device in closed case, sensor obtains real-time environment ginseng
Number, microscope can timely obtain the state of cell growth, while can carry out shirtsleeve operation by mechanical arm, as far as possible
The intracorporal environment of guard box stabilization.
The reality of single-factor influence experiment can be improved in the stability and conventional efficient for improving the environment of cell culture experiments
The precision for testing result ensure that the accuracy of experimental data.
Preferably, first tampering devic includes culture medium regulating device, and the culture medium regulating device includes pH tune
It is same that regulating device, regulating current device and medicament adjusting device, the pH regulating device and medicament adjusting device pass through conduit respectively
Cultivation region connection, the regulating current device include multiple power supplys, and the anode and cathode of the power supply are flexibly connected with culture dish；
The medicament adjusting device conveys growth factor, drug, extract, hormone, forerunner into culture medium by exogenous conduit
Body, product promotor it is one of or several.First tampering devic can be influenced by adjusting the pH value in culture dish
The growth of cell, drug adding set can to culture medium intracellular addition drug to observe drug to influence, such as observe
Influence of the drug to cancer cell；Portion's adding set mainly passes through conduit and connects with cultivation region.
Preferably, the pH regulating device includes sour regulating device and alkali regulating device, and the acid regulating device includes acid
Pump, acid liquor storage tank, acid solution conduit, sour controller, sour loader, the acid loader is the same as sour controller connection, the acid control
Device processed is the same as acid pump connection, the acid pump connection acid solution conduit and acid liquor storage tank；The alkali regulating device includes alkali pump, lye storage
Tank, lye conduit, alkali controller, alkali loader are deposited, the alkali loader is connected with alkali controller, the same alkali pump of alkali controller
Connection, the alkali pump connection lye conduit and alkali storage tank；Acid buffer system, strong acid are stored in the acid liquor storage tank
Or weak acid is one of or several, is stored with alkaline buffer system in the lye storage holding vessel, highly basic, weak base are wherein
It is one or more of.
Preferably, the medicament adjusting device includes Teat pipette, liquid medicine storage tank, liquid medicine catheter, medicine controller, medicine input
Device, the medicine loader are connected with medicine controller, and the medicine controller is connected with Teat pipette, the Teat pipette connection liquid medicine catheter and medicine
Liquid holding vessel.
Preferably, second tampering devic includes light regulating device, temperature-adjusting device, humidity control apparatus, gas
Regulating device, the air regulating device include carbon dioxide regulating device, oxygen regulating device, nitrogen regulating device.Second
Environment inside the main adjustment equipment of tampering devic.
Preferably, the light intensity and color in the smooth regulating device adjustable case body；Second tampering devic further includes
Sound wave generating device, electromagnetic wave generating device, negative ion generating device, ray generating device it is one of or several, it is described
The producible ray of ray generating device includes infrared ray.The growth of cell is influenced by applying sound field or magnetic field.
Preferably, the smooth regulating device is the light source of adjustable wavelength or intensity, and the temperature-adjusting device is air-conditioning,
The humidity control apparatus is humidification dehumidifying integrated machine；The air regulating device includes carbon dioxide regulating device, oxygen tune
Regulating device, nitrogen regulating device, the carbon dioxide regulating device include carbon dioxide air pump, carbon dioxide gas tank, and described two
Carbonoxide air pump is connected with carbon dioxide gas tank, and the carbon dioxide air pump is connected with closed case；The oxygen regulating device
Including oxygen pump, oxygen tank, the oxygen tank is pumped with oxygen and is connected, and the oxygen pump is connected with closed case；The nitrogen gas tank
It pumps and connects with nitrogen, the nitrogen pump is connected with closed case.Environment regulating device is for keeping the intracorporal culture environment of case steady
It is fixed.
Preferably, the exogenous conduit is connected with sampler.
Preferably, the cultivation region is connected with nutrient solution adding device, and the nutrient solution adding device passes through with cultivation region
The connection of external source formula conduit；Nutrient solution is stored in the nutrient solution adding device, the nutrient solution includes microelement, mineral
Matter, amino acid, vitamin, sugar, fatty acid, inorganic salts, organic nitrogen, inorganic nitrogen, serum, natural extract, protein, organic matter
It is one of or several.
It preferably, further include control device, the control device includes processing unit, operation panel, and the processing unit is same
Second tampering devic and the connection of the first tampering devic, the processing unit are connected with the operation panel.Multiple press is set on control panel
Button, for integrated control inside and the first tampering devic.Control device can control the intracorporal environment of case, facilitate operator logical
Input parameter is crossed the intracorporal environment of case is adjusted.
Preferably, detection device is provided in the closed case, the detection device includes spectrometer, microscope, temperature
Spend table, humidometer, gas concentration table.
Preferably, cabinet door and observation window are provided in the closed case；The microscope is connected with display, described aobvious
Show that the outside of closed case is arranged in device.
Preferably, flow sensor is provided on the external source formula conduit.
A kind of Synthetical cultivation method, comprising:
It will be cultivated in the certain environment of culture dish containing cell, drug added into cultivation region by medicament adjusting device；Institute
Stating certain environment includes sound wave environment, electromagnetic wave environment, acidic environment, alkaline environment, hot environment, low temperature environment, high humidity
Environment, low moisture environments, anaerobic environment, oxygen-enriched environment, hypocapnia environment, carbon dioxide enriched environment, nitrogen stress environment, rich azo-cycle
Border is one of or several；
It is equipped with photographic device in microscope, cultivates after a certain period of time, after the photographic device obtains the amplified image of microscope
Image is transmitted on display；The cell be zooblast, plant cell, fungal cell, bacterium, thallus, polypide its
Middle one or more；
After the completion of shooting, continue to cultivate or cultivate again.
Compared with prior art, the device have the advantages that are as follows: improve the stabilization of the environment of cell culture experiments
Property and conventional efficient, can be improved single-factor influence experiment experimental result precision, ensure that the accuracy of experimental data；It can
It is declared so that whether verification portion drug plays the role of it to cell.
Detailed description of the invention
Fig. 1 is a kind of schematic diagram of Synthetical cultivation system.
Fig. 2 is a kind of another schematic diagram of Synthetical cultivation system.
Specific embodiment
Following implementation column is to further explanation of the invention, is not limitation of the present invention.
Embodiment 1
A kind of Synthetical cultivation system, including closed case 1, microscope 2, cultivation region 3, display 4, mechanical arm 5, tampering devic,
The microscope 2, cultivation region 3, mechanical arm 5 are arranged in closed case 1, and the display 4 is connected with microscope 2, the training
Support the observation area that microscope 2 is arranged in area 3；Culture dish is provided in the cultivation region 3, the quantity of the culture dish is 1；Institute
Stating culture tampering devic includes the first tampering devic and the second tampering devic, and first tampering devic is connected with cultivation region 3, institute
The second tampering devic is stated for adjusting the environment in the outer incubator of culture dish；The closed case by metal, plastics, glass its
Middle one or more material is made, and is provided with observation window in the closed case 1.First tampering devic includes that pH adjusts dress
It sets, regulating current device and medicament adjusting device, the pH regulating device and medicament adjusting device pass through conduit with culture respectively
Area's connection, the regulating current device 66 include multiple power supplys, and the anode and cathode of the power supply are flexibly connected with culture dish.Institute
Stating pH regulating device includes sour regulating device and alkali regulating device, and the acid regulating device includes 611, acid liquor storage tank of acid pump
612, acid solution conduit 613, sour controller 614, sour loader 615, the acid loader 615 is connected with sour controller 614, described
Sour controller 614 is the same as 611 connection of acid pump, the 611 connection acid solution conduit 613 of acid pump and acid liquor storage tank 612；The alkali is adjusted
Device includes alkali pump 621, alkali storage tank 622, lye conduit 623, alkali controller 624, alkali loader 625, the alkali input
Device 625 is connected with alkali controller 624, and the alkali controller 624 is connected with alkali pump 621, and the alkali pump 621 connects lye conduit
623 and alkali storage tank 622；Acid buffer system is stored in the acid liquor storage tank 612, the lye stores holding vessel
Alkaline buffer system is stored in 622.The medicament adjusting device includes Teat pipette 631, liquid medicine storage tank 632, liquid medicine catheter
633, medicine controller 634, medicine loader 635, the medicine loader 635 are connected with medicine controller 634, the medicine controller 634
It is connected with Teat pipette 631, the Teat pipette 631 connects liquid medicine catheter 633 and liquid medicine storage tank 632.Second tampering devic includes
Sound wave generating device 64, electromagnetic wave generating device 65.Second tampering devic further includes light regulating device 71, temperature adjusting dress
72, humidity control apparatus 73, air regulating device are set, the smooth regulating device 71 is the light source of adjustable wavelength or intensity, institute
Stating temperature-adjusting device 72 is air-conditioning, and the humidity control apparatus 73 is humidification dehumidifying integrated machine；The air regulating device packet
Carbon dioxide regulating device, oxygen regulating device, nitrogen regulating device are included, the carbon dioxide regulating device includes carbon dioxide
Air pump 741, carbon dioxide gas tank 742, the carbon dioxide air pump 741 are connected with carbon dioxide gas tank 742, the carbon dioxide
Air pump 741 is connected with closed case 1；The oxygen regulating device includes that oxygen pumps 751, oxygen tank 752, the oxygen tank 752
With 751 connection of oxygen pump, the oxygen pump 751 is connected with closed case 1；The nitrogen gas tank 762 is the same as 761 connection of nitrogen pump, institute
Nitrogen pump 761 is stated to connect with closed case 1.The cultivation region 3 is connected with nutrient solution adding device, the nutrient solution adding device
Including nutrient pump 91, standing reservoir tank 92, the standing reservoir tank 92 is connected with nutrient pump 91, the nutrient pump
91 are connected with cultivation region 3 by external source formula conduit；Nutrient solution is stored in the standing reservoir tank 92, the nutrient solution includes
Microelement, minerals, amino acid, vitamin, sugar, protein, organic matter.It is provided with detection device in the closed case,
The detection device includes spectrometer, thermometer 81, humidometer 82, gas concentration table 83.The environment regulating device further includes
Control device, the control device include operation panel 78 and processing unit 77, and the operation panel 78 is connected with processing unit 77, institute
Processing unit 77 is stated with light source 71, air-conditioning 72, humidification dehumidifying integrated machine 73, carbon dioxide air pump 741, oxygen pump 751, nitrogen pump
761 connections；The synthermal table 81 of the processing unit 77, humidometer 82, gas concentration table 83 connect.It is set on the external source formula conduit
It is equipped with flowmeter.The external source formula conduit is connected with sampler, the substance in the available culture medium of sampler.
Culture environment is adjusted by environment regulating device in closed case, sensor obtains real-time environment ginseng
Number, microscope can timely obtain the state of cell growth, while can carry out shirtsleeve operation by mechanical arm, as far as possible
The intracorporal environment of guard box stabilization.The stability and conventional efficient for improving the environment of cell culture experiments, can be improved
The precision of the experimental result of single-factor influence experiment, ensure that the accuracy of experimental data.First tampering devic can pass through tune
PH value in section culture dish influences the growth of cell, drug adding set can to culture medium intracellular addition drug with
Drug is observed to influence, such as influence of the observation drug to cancer cell.Second tampering devic is by applying sound field or magnetic field come shadow
The growth of cell is rung, and for keeping the intracorporal culture environment of case to stablize.Control device can control the intracorporal environment of case, convenient
Operator is adjusted the intracorporal environment of case by inputting parameter.
Embodiment 2
A kind of Synthetical cultivation system, including closed case 1, microscope 2, cultivation region 3, display 4, mechanical arm 5, tampering devic,
The microscope 2, cultivation region 3, mechanical arm 5 are arranged in closed case 1, and the display 4 is connected with microscope 2, the training
Support the observation area that microscope 2 is arranged in area 3；Culture dish is provided in the cultivation region 3, the quantity of the culture dish is 1；Institute
Stating culture tampering devic includes the first tampering devic and the second tampering devic, and first tampering devic is connected with cultivation region 3, institute
The second tampering devic is stated for adjusting the environment in the outer incubator of culture dish；The closed case by metal, plastics, glass its
Middle one or more material is made, and is provided with observation window in the closed case 1.
Embodiment 3
A kind of Synthetical cultivation system, including closed case 1, microscope 2, cultivation region 3, display 4, mechanical arm 5, tampering devic,
The microscope 2, cultivation region 3, mechanical arm 5 are arranged in closed case 1, and the display 4 is connected with microscope 2, the training
Support the observation area that microscope 2 is arranged in area 3；Culture dish is provided in the cultivation region 3, the quantity of the culture dish is 1；Institute
Stating culture tampering devic includes the first tampering devic and the second tampering devic, and first tampering devic is connected with cultivation region 3, institute
The second tampering devic is stated for adjusting the environment in the outer incubator of culture dish；The closed case by metal, plastics, glass its
Middle one or more material is made, and is provided with observation window in the closed case 1.First tampering devic includes that pH adjusts dress
It sets, regulating current device and medicament adjusting device, the pH regulating device and medicament adjusting device pass through conduit with culture respectively
Area's connection, the regulating current device 66 include multiple power supplys, and the anode and cathode of the power supply are flexibly connected with culture dish.Institute
Stating pH regulating device includes sour regulating device and alkali regulating device, and the acid regulating device includes 611, acid liquor storage tank of acid pump
612, acid solution conduit 613, sour controller 614, sour loader 615, the acid loader 615 is connected with sour controller 614, described
Sour controller 614 is the same as 611 connection of acid pump, the 611 connection acid solution conduit 613 of acid pump and acid liquor storage tank 612；The alkali is adjusted
Device includes alkali pump 621, alkali storage tank 622, lye conduit 623, alkali controller 624, alkali loader 625, the alkali input
Device 625 is connected with alkali controller 624, and the alkali controller 624 is connected with alkali pump 621, and the alkali pump 621 connects lye conduit
623 and alkali storage tank 622；The one of which of acid buffer system, strong acid or weak acid is stored in the acid liquor storage tank 612
Or it is several, be stored with alkaline buffer system in lye storage holding vessel 622, highly basic, weak base it is one of or several.Institute
Stating medicament adjusting device includes Teat pipette 631, liquid medicine storage tank 632, liquid medicine catheter 633, medicine controller 634, medicine loader 635,
The medicine loader 635 is connected with medicine controller 634, and the medicine controller 634 is connected with Teat pipette 631, and the Teat pipette 631 connects
Liquid medicine catheter 633 and liquid medicine storage tank 632.Second tampering devic includes sound wave generating device 64, electromagnetic wave generating device
65.Second tampering devic includes light regulating device 71, temperature-adjusting device 72, humidity control apparatus 73, gas regulation dress
It sets, the smooth regulating device 71 is the light source of adjustable color, wavelength or intensity, and the temperature-adjusting device 72 is air-conditioning, institute
Humidity control apparatus 73 is stated as humidification dehumidifying integrated machine；The air regulating device includes carbon dioxide regulating device, oxygen tune
Regulating device, nitrogen regulating device, the carbon dioxide regulating device include carbon dioxide air pump 741, carbon dioxide gas tank 742,
The carbon dioxide air pump 741 is connected with carbon dioxide gas tank 742, and the carbon dioxide air pump 741 is connected with closed case 1；
The oxygen regulating device includes that oxygen pumps 751, oxygen tank 752, and the oxygen tank 752 is the same as 751 connection of oxygen pump, the oxygen
Pump 751 is connected with closed case 1；The nitrogen gas tank 762 connects with 761 connection of nitrogen pump, the nitrogen pump 761 with closed case 1
It connects.The cultivation region 3 is connected with nutrient solution adding device, and the nutrient solution adding device includes nutrient pump 91, nutrient solution storage
Tank 92 is deposited, the standing reservoir tank 92 is connected with nutrient pump 91, and the nutrient pump 91 is led with cultivation region 3 by external source formula
Pipe connection；Store nutrient solution in the standing reservoir tank 92, the nutrient solution include microelement, minerals, amino acid,
Vitamin, sugar, protein, organic matter it is one of or several.Detection device, the detection are provided in the closed case
Device includes spectrometer, thermometer 81, humidometer 82, gas concentration table 83.
Manual adjustment can be carried out according to the data of thermometer, humidometer, gas concentration table.
Embodiment 4
A kind of Synthetical cultivation system, including closed case 1, microscope 2, cultivation region 3, display 4, mechanical arm 5, tampering devic,
The microscope 2, cultivation region 3, mechanical arm 5 are arranged in closed case 1, and the display 4 is connected with microscope 2, the training
Support the observation area that microscope 2 is arranged in area 3；Culture dish is provided in the cultivation region 3, the quantity of the culture dish is 1, institute
The quantity for stating microscope 2 is 1；The culture tampering devic include the first tampering devic and the second tampering devic, described first
Tampering devic is connected with cultivation region 3, and second tampering devic is used to adjust the environment in the outer incubator of culture dish；Described first
Tampering devic is connected with cultivation region, and second tampering devic is used to adjust the environment in the outer incubator of culture dish, and described first
Tampering devic is connected with cultivation region by exogenous conduit；The closed case by metal, plastics, glass it is one of or several
Kind material is made, and is provided with observation window in the closed case 1.First tampering devic includes culture medium regulating device, institute
Stating culture medium regulating device includes pH regulating device 61, regulating current device 66 and medicament adjusting device 63, and the pH adjusts dress
Set 61 and medicament adjusting device 63 respectively by conduit with cultivation region 3 connect, the regulating current device 66 include multiple power supplys,
The anode and cathode of the power supply are flexibly connected with culture dish.Second tampering devic includes light regulating device 71, temperature tune
Regulating device 72, humidity control apparatus 73, air regulating device, the air regulating device include carbon dioxide regulating device 74,
Oxygen regulating device 75, nitrogen regulating device 76.Second tampering devic further includes sound wave generating device 64 and electromagnetic wave hair
Generating apparatus 65.Light intensity and color in 71 adjustable case body of smooth regulating device；Second tampering devic further include bear from
Sub- generating device, ray generating device, the producible ray of ray generating device includes infrared ray.System further includes control
Device, the control device include processing unit, operation panel, and the processing unit is the same as the second tampering devic and the first tampering devic
Connection, the processing unit are connected with the operation panel.Cabinet door and observation window are provided in the closed case；The microscope is same
The outside of closed case is arranged in display connection, the display.The cultivation region is connected with nutrient solution adding device, described
Nutrient solution adding device is connected with cultivation region by external source formula conduit；Nutrient solution, institute are stored in the nutrient solution adding device
Stating nutrient solution includes microelement, minerals, amino acid, vitamin, sugar, fatty acid, inorganic salts, organic nitrogen, inorganic nitrogen, blood
Clearly, natural extract, protein, organic matter it is one of or several.The detection device include spectrometer, thermometer 81,
Humidometer 82, gas concentration table 83, negative ion concentration detection device, light intensity instrument.The exogenous conduit is connected with sampler.
It is connected on the conduit with sampler, is provided with flow sensor on the conduit.The same sampler of the conduit, nutrient solution
Adding set, drug adding set are led to by four-way or five to be connect.
Embodiment 5
A kind of Synthetical cultivation method, comprising:
It will be cultivated in the certain environment of culture dish containing cell, drug added into cultivation region by medicament adjusting device；Institute
Stating certain environment includes sound wave environment, electromagnetic wave environment, acidic environment, alkaline environment, hot environment, low temperature environment, high humidity
Environment, low moisture environments, anaerobic environment, oxygen-enriched environment, hypocapnia environment, carbon dioxide enriched environment, nitrogen stress environment, rich azo-cycle
Border is one of or several；
It is equipped with photographic device in microscope, cultivates after a certain period of time, after the photographic device obtains the amplified image of microscope
Image is transmitted on display；The cell be zooblast, plant cell, fungal cell, bacterium, thallus, polypide its
Middle one or more；
After the completion of shooting, continue to cultivate or cultivate again.
Above-listed detailed description is illustrating for possible embodiments of the present invention, and above embodiments are not to limit this
The scope of the patents of invention, all equivalence enforcements or change without departing from carried out by the present invention, is intended to be limited solely by the scope of the patents of this case.
Claims (10)
1. a kind of Synthetical cultivation system, which is characterized in that including closed case, microscope, cultivation region, mechanical arm, tampering devic,
The microscope, cultivation region, mechanical arm are arranged in closed case, and the cultivation region is arranged in microscopical observation area；It is described
Culture dish, microscopical quantity >=1, quantity >=1 of the culture dish are provided in cultivation region；The tampering devic includes
First tampering devic and the second tampering devic, first tampering devic are connected with cultivation region, and second tampering devic is used for
The environment in the outer incubator of culture dish is adjusted, first tampering devic is connected with cultivation region by the exogenous conduit of at least one
It connects；The closed case is made of metal, plastics, the one of which of glass or different materials.
2. a kind of Synthetical cultivation system according to claim 1, which is characterized in that first tampering devic includes culture
Keynote regulating device, the culture medium regulating device include pH regulating device, regulating current device and medicament adjusting device, the pH
Regulating device passes through conduit with medicament adjusting device respectively and connects with cultivation region, and the regulating current device includes multiple power supplys,
The anode and cathode of the power supply are flexibly connected with culture dish；The medicament adjusting device is by exogenous conduit into culture medium
Convey growth factor, drug, extract, hormone, presoma, product promotor it is one of or several.
3. a kind of Synthetical cultivation system according to claim 2, which is characterized in that second tampering devic includes light tune
Regulating device, temperature-adjusting device, humidity control apparatus, air regulating device, the air regulating device include carbon dioxide tune
Regulating device, oxygen regulating device, nitrogen regulating device；Light intensity and color in the smooth regulating device adjustable case body；It is described
Second tampering devic further include sound wave generating device, electromagnetic wave generating device, negative ion generating device, ray generating device its
Middle one or more, the producible ray of ray generating device includes infrared ray.
4. a kind of Synthetical cultivation system according to claim 2, which is characterized in that the exogenous same sampler of conduit
Connection.
5. a kind of Synthetical cultivation system according to claim 1, which is characterized in that it further include control device, the control
Device includes processing unit, operation panel, and the processing unit is connected with the second tampering devic and the first tampering devic, the processing
Unit is connected with the operation panel.
6. a kind of Synthetical cultivation system according to claim 1, which is characterized in that be provided with cabinet door in the closed case
And observation window；The microscope is connected with display, and the outside of closed case is arranged in the display.
7. a kind of Synthetical cultivation system according to claim 1, which is characterized in that add and fill with nutrient solution in the cultivation region
Connection is set, the nutrient solution adding device is connected with cultivation region by external source formula conduit；It is stored in the nutrient solution adding device
Nutritious liquid, the nutrient solution include microelement, minerals, amino acid, vitamin, sugar, fatty acid, inorganic salts, organic nitrogen,
Inorganic nitrogen, serum, natural extract, protein, organic matter it is one of or several.
8. a kind of Synthetical cultivation system according to claim 1, which is characterized in that be provided with detection in the closed case
Device, the detection device include spectrometer, microscope, thermometer, humidometer, gas concentration table, negative ion concentration detection dress
It sets, light intensity instrument.
9. a kind of Synthetical cultivation system according to claim 1, which is characterized in that be provided with stream on the external source formula conduit
Quantity sensor.
10. a kind of Synthetical cultivation method characterized by comprising
It will be cultivated in the certain environment of culture dish containing cell, drug added into cultivation region by medicament adjusting device；Institute
Stating certain environment includes sound wave environment, electromagnetic wave environment, acidic environment, alkaline environment, hot environment, low temperature environment, high humidity
Environment, low moisture environments, anaerobic environment, oxygen-enriched environment, hypocapnia environment, carbon dioxide enriched environment, nitrogen stress environment, rich azo-cycle
Border is one of or several；
It is equipped with photographic device in microscope, cultivates after a certain period of time, after the photographic device obtains the amplified image of microscope
Image is transmitted on display；The cell be zooblast, plant cell, fungal cell, bacterium, thallus, polypide its
Middle one or more；
After the completion of shooting, continue to cultivate or cultivate again.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115125127A (en) * | 2022-07-13 | 2022-09-30 | 广州逆熵电子科技有限公司 | Method and device for observing full growth period of microorganisms |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201695044U (en) * | 2010-05-17 | 2011-01-05 | 复旦大学 | Medicine filling and perfusion device for closed culture chamber |
| CN102174395A (en) * | 2011-01-30 | 2011-09-07 | 中国科学院广州生物医药与健康研究院 | Automation augmentation and culture system of induced pluripotent stem cells |
| US20110263008A1 (en) * | 2010-04-26 | 2011-10-27 | National Changhua University Of Education | Cell Culture Real-Time Observation System |
| CN102712886A (en) * | 2009-01-30 | 2012-10-03 | 埃米利奥·马特森塔曼斯 | Method and modular equipment for the processing and storage of cell cultures |
| CN104480010A (en) * | 2014-11-28 | 2015-04-01 | 清华大学 | Biological reaction device and application thereof |
| CN206014933U (en) * | 2016-09-19 | 2017-03-15 | 济宁学院 | It is easy to the microbial cultivation device for sampling observation |
| JP2019017340A (en) * | 2017-07-20 | 2019-02-07 | 学校法人近畿大学 | Cell culture observation device and cell observation unit |
-
2019
- 2019-07-27 CN CN201910685527.5A patent/CN110373323A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102712886A (en) * | 2009-01-30 | 2012-10-03 | 埃米利奥·马特森塔曼斯 | Method and modular equipment for the processing and storage of cell cultures |
| US20110263008A1 (en) * | 2010-04-26 | 2011-10-27 | National Changhua University Of Education | Cell Culture Real-Time Observation System |
| CN201695044U (en) * | 2010-05-17 | 2011-01-05 | 复旦大学 | Medicine filling and perfusion device for closed culture chamber |
| CN102174395A (en) * | 2011-01-30 | 2011-09-07 | 中国科学院广州生物医药与健康研究院 | Automation augmentation and culture system of induced pluripotent stem cells |
| CN104480010A (en) * | 2014-11-28 | 2015-04-01 | 清华大学 | Biological reaction device and application thereof |
| CN206014933U (en) * | 2016-09-19 | 2017-03-15 | 济宁学院 | It is easy to the microbial cultivation device for sampling observation |
| JP2019017340A (en) * | 2017-07-20 | 2019-02-07 | 学校法人近畿大学 | Cell culture observation device and cell observation unit |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115125127A (en) * | 2022-07-13 | 2022-09-30 | 广州逆熵电子科技有限公司 | Method and device for observing full growth period of microorganisms |
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Application publication date: 20191025 |