CN113514644A

CN113514644A - Detection chip is selected for use to quick open-close type antibody screening that facilitates use

Info

Publication number: CN113514644A
Application number: CN202110468447.1A
Authority: CN
Inventors: 林琳; 皮付伟; 戴春阳; 刘婧涵; 刘建
Original assignee: Zhongnong Kangzheng Technology Service Co ltd
Current assignee: Zhongnong Kangzheng Technology Service Co ltd
Priority date: 2021-04-28
Filing date: 2021-04-28
Publication date: 2021-10-19
Anticipated expiration: 2041-04-28
Also published as: CN113514644B

Abstract

The invention relates to the technical field of lab-on-a-chip, and discloses a detection chip for screening an antibody, which is convenient to use and can be opened and closed quickly, and the detection chip comprises a box body, a box body and biological chips, wherein a plurality of accommodating cavities are formed in one side of the interior of the box body at equal intervals, the box body is arranged in each accommodating cavity, two groups of accommodating cavities are formed in the interior of the box body at equal intervals, the biological chips are arranged in the accommodating cavities, each biological chip comprises a chip body, a positioning pin and a titration hole, slots are formed right above the accommodating cavities, and transparent cover plates are arranged in the slots. The invention not only improves the efficiency of taking and placing the biological chips, but also adopts the positioning modes of elastic adsorption and elastic contact on the two side surfaces of the biological chips respectively, thereby improving the safety of the biological chips during storage and use and improving the efficiency of taking and placing the box body with a plurality of groups of biological chips.

Description

Detection chip is selected for use to quick open-close type antibody screening that facilitates use

Technical Field

The invention relates to the technical field of lab-on-a-chip, in particular to a quick-opening-closing type detection chip for screening antibodies, which is convenient to use.

Background

Lab-on-a-chip, also known as micro total analysis system, is a technology for cross-implementing chemical analysis and detection by analytical chemistry, micro electro mechanical processing, computer, electronics, material science and biology, medicine, engineering, etc. For example, when the technology is used for screening and detecting biological antibodies, the related basic operation units such as sample preparation, biological and chemical reactions, separation and detection and the like are integrated on a chip with the square centimeter, so that different biological or chemical reaction processes can be completed at one time, and the products can be analyzed, so that the detection efficiency is greatly improved.

And in the actual operation in-process, to different experimental purpose and design parameter, need choose for use the biological detection chip of multiple different structures to carry out multiunit testing, among the prior art, this type of biological detection chip is generally concentrated through supporting kit and is accomodate, but the inner structure of many kits is complicated, lead to choosing for use of biological detection chip, it is comparatively troublesome to get and put, biological detection chip probably takes place to rock in the kit simultaneously, lead to the corner impaired, in addition, kit and detecting instrument also separately deposit usually, it is equally comparatively loaded down with trivial details to get the flow of putting around experimental each time.

Therefore, the person skilled in the art provides a convenient detection chip for screening antibodies with quick open and close, so as to solve the problems mentioned in the background art.

Disclosure of Invention

The invention aims to provide a quick open-close type antibody screening detection chip which is convenient to use, so as to solve the problems in the background technology.

In order to achieve the purpose, the invention provides the following technical scheme:

a detection chip for screening a quick open-close type antibody, which is convenient to use, comprises a box body, a box body and a biological chip, wherein a plurality of accommodating cavities are arranged on one side in the box body at equal intervals, one side of each accommodating cavity horizontally extends to the outside of the box body, the box body is arranged in each accommodating cavity, two groups of accommodating cavities are arranged in the box body at equal intervals, the biological chip is arranged in each accommodating cavity, the biological chip comprises a chip body, positioning pins and titration holes, a plurality of positioning pins are welded on two outer side walls of the chip body at equal intervals, the chip body is of a hollow structure, the titration holes are arranged on one side of the top end of the chip body, slots are arranged right above the accommodating cavities, and one sides of the slots horizontally extend to the outside of the box body, transparent cover plates are arranged in the slots.

As a still further scheme of the invention: accept the both sides of chamber bottom and all horizontally seted up the spout, just one side of spout all horizontally extends to the outside of box for with a plurality of a set of rubber gyro wheel mutual adaptation.

As a still further scheme of the invention: it has two first magnetism to inhale the piece all to inlay symmetrically to accept one side of chamber bottom, just the surface of first magnetism inhale the piece all with it flushes each other to accept the inside wall in chamber for inhale the mutual adaptation of piece with the second magnetism, and avoid with the box body bumps.

As a still further scheme of the invention: a plurality of recess has all been seted up to equidistant on the both sides wall of box body bottom, just the inside of recess all rotates and is connected with the rubber gyro wheel, the equal level in one side of rubber gyro wheel extends to the outside of box body, two sets of the rubber gyro wheel respectively with two sets of the spout corresponds the setting, just one side of rubber gyro wheel all with the inside wall rolling contact of spout is used for supplementary injecing the translation orbit of box body to reduce the contact friction, thereby avoid the box body takes place to squint or card at the pull in-process and pauses.

As a still further scheme of the invention: one side of box body bottom all the symmetry is inlayed and is had two second magnetism to inhale the piece, just first magnetism inhale the piece all with second magnetism inhale the piece and correspond the setting and mutual actuation for place the back automatic locking that targets in place the box body to maintain relative sealed, and avoid the box body removes by oneself and leads to dropping.

As a still further scheme of the invention: heavy groove has all been seted up at the middle part of box body one side, just all inlay in heavy groove and have the handle, and the both ends of handle all rotate connect in heavy groove, heavy groove with the handle all is half circular, is convenient for manually take out and put the box body to reduce the unnecessary space and occupy.

As a still further scheme of the invention: the central point of holding chamber bottom puts the department and all attaches with first gasket, just first gasket all is the silica gel material, the top of first gasket all with the bottom elasticity of chip body adsorbs, avoids the chip body is in take place the displacement in the holding chamber or receive the collision damage.

As a still further scheme of the invention: two first shrinkage pools have all been seted up to one side at slot top the symmetry, two second shrinkage pools have all been seted up to the opposite side at slot top the symmetry for with protruding mutual adaptation.

As a still further scheme of the invention: transparent cover plate all with slot slip gomphosis constitutes limit structure, transparent cover plate all is the toughened glass material, the central point department of putting of transparent cover plate bottom all pastes with the second gasket, just the second gasket all is the sponge material, the bottom of second gasket all with the top elastic contact of chip body has certain elastic shrinkage space, and keeps the gentle and agreeable of contact surface during sliding contact, thereby avoids the chip body receives extrusion or friction damage.

As a still further scheme of the invention: the equal symmetry in one side on transparent cover piece top is provided with two archs, just the arch respectively with first shrinkage pool the mutual gomphosis of second shrinkage pool constitutes limit structure, is used for injecing respectively the maximum depth of insertion and the biggest outer range of drawing of transparent cover piece avoid transparent cover piece takes place to rock or drop.

Compared with the prior art, the invention has the beneficial effects that:

1. the transparent cover plate is slightly pressed to enable the protrusions to be separated from the first concave holes, then the transparent cover plate is pushed and pushed in an outward sliding mode until the protrusions and the second concave holes are embedded and positioned, at the moment, the biochip clamp can be taken out from the accommodating cavity and then placed on a test table at the top end of the box body to perform detection work, after the detection is completed, the biochip can be sealed and stored by reversely operating the processes, and therefore the biochip taking and placing efficiency is improved;

2. the first gasket made of silica gel is elastically adsorbed to the bottom end of the chip body, so that the chip body is prevented from shaking in the accommodating cavity, the second gasket made of sponge is elastically contacted with the top end of the chip body, extrusion or friction damage is avoided, the two groups of positioning pins are convenient for accurately positioning the chip body, and the deviation of the placement position is avoided, so that the safety of the biological chip during storage and use is improved;

3. through the rolling contact between two sets of rubber gyro wheels and the spout, be convenient for insert the box body stably and accept the chamber in, when first magnetism piece of inhaling was inhaled the piece with the second magnetism and is inhaled the piece and correspond when inhaling, showed that the box body is accomodate completely promptly, or through the handle in the top spin heavy groove, can pull out the box body reverse hard to the efficiency of getting of box body has been improved.

Drawings

FIG. 1 is a schematic perspective view of a conveniently used detection chip for screening an antibody in a rapid open/close manner;

FIG. 2 is an enlarged schematic view of the detection chip for screening rapid open/close antibody, which is convenient to use and is shown in the position A of FIG. 1;

FIG. 3 is a schematic diagram of an expanded structure of a case of a conveniently used rapid open/close antibody screening detection chip;

FIG. 4 is a schematic view of a partial structure of a case of a rapid open/close antibody screening test chip for convenient use;

FIG. 5 is a schematic diagram of an explosion structure of a receiving cavity in a detection chip for conveniently used rapid open/close antibody screening;

FIG. 6 is an enlarged schematic view of a biochip in a rapid start-stop antibody screening test chip which is convenient to use.

In the figure: 1. a box body; 2. an accommodating cavity; 3. a chute; 4. a first magnetic attraction block; 5. a box body; 6. a groove; 7. a rubber roller; 8. a second magnetic attraction block; 9. sinking a groove; 10. a handle; 11. an accommodating cavity; 12. a first gasket; 13. a biochip; 1301. a chip body; 1302. positioning a pin; 1303. a titration well; 14. a slot; 15. a first recess; 16. a second recess; 17. a transparent cover sheet; 18. a second gasket; 19. and (4) protruding.

Detailed Description

Referring to fig. 1 to 6, in the embodiment of the invention, a conveniently used detection chip for screening an antibody in a rapid open/close manner comprises a box body 1, a box body 5 and a biochip 13, wherein a plurality of accommodating cavities 2 are arranged at equal intervals on one side inside the box body 1, one side of each accommodating cavity 2 horizontally extends to the outside of the box body 1, the box body 5 is arranged inside each accommodating cavity 2, two groups of accommodating cavities 11 are arranged at equal intervals inside the box body 5, the biochip 13 is arranged inside each accommodating cavity 11, each biochip 13 comprises a chip body 1301, positioning pins 1302 and titration holes 1303, a plurality of positioning pins 1302 are welded at equal intervals on two outer side walls of the chip body 1301, the chip body 1301 is of a hollow structure, the titration holes are arranged on one side of the top end of the chip body 1301, slots 14 are arranged right above the accommodating cavities 11, and one side of each slot 14 horizontally extends to the outside of the box body 5, the slots 14 are each provided with a transparent cover 17.

In fig. 1, 2, 3 and 4: the two sides of the bottom of the accommodating cavity 2 are both horizontally provided with sliding chutes 3, and one sides of the sliding chutes 3 are both horizontally extended to the outside of the box body 1 and are used for being matched with a plurality of groups of rubber rollers 7; two first magnetic blocks 4 are symmetrically embedded on one side of the bottom of the accommodating cavity 2, and the surfaces of the first magnetic blocks 4 are flush with the inner side wall of the accommodating cavity 2 and are used for being matched with the second magnetic blocks 8 and avoiding collision with the box body 5; the two side walls of the bottom of the box body 5 are provided with a plurality of grooves 6 at equal intervals, the insides of the grooves 6 are rotatably connected with rubber rollers 7, one sides of the rubber rollers 7 horizontally extend to the outside of the box body 5, the two groups of rubber rollers 7 are respectively arranged corresponding to the two groups of chutes 3, and one sides of the rubber rollers 7 are in rolling contact with the inner side walls of the chutes 3 to be used for assisting in limiting the translation track of the box body 5 and reducing contact friction, so that the box body 5 is prevented from shifting or jamming in the drawing process; two second magnetic blocks 8 are symmetrically embedded on one side of the bottom of the box body 5, and the first magnetic blocks 4 are arranged corresponding to the second magnetic blocks 8 and mutually attracted for automatically locking the box body 5 after being placed in place, so that relative sealing is maintained, and the box body 5 is prevented from falling off due to self-movement; heavy groove 9 has all been seted up at the middle part of box body 5 one side, and all inlayed handle 10 in heavy groove 9 to the both ends of handle 10 are all rotated and are connected in heavy groove 9, and heavy groove 9 all is half annular with handle 10, and the manual box body 5 of putting of taking out of being convenient for reduces the unnecessary space and occupies.

In fig. 2, 3 and 5: the first gaskets 12 are attached to the central positions of the bottom ends of the accommodating cavities 11, the first gaskets 12 are made of silica gel, the top ends of the first gaskets 12 are elastically adsorbed to the bottom ends of the chip bodies 1301, and the chip bodies 1301 are prevented from displacing or being damaged by collision in the accommodating cavities 11; transparent cover 17 all slides the gomphosis with slot 14 and constitutes limit structure, and transparent cover 17 all is the toughened glass material, and the central point department of putting of transparent cover 17 bottom all pastes with second gasket 18, and second gasket 18 all is the sponge material, and the bottom of second gasket 18 all with the top elastic contact of chip body 1301, has certain elastic shrinkage space, and keeps the compliance of contact surface during sliding contact to avoid chip body 1301 to receive extrusion or frictional damage.

In fig. 3 and 5: two first concave holes 15 are symmetrically formed in one side of the top of the slot 14, and two second concave holes 16 are symmetrically formed in the other side of the top of the slot 14 and are used for being matched with the protrusions 19; one side on transparent cover 17 top all is provided with two archs 19 symmetrically, and protruding 19 respectively with first shrinkage pool 15, the mutual gomphosis of second shrinkage pool 16 and constitute limit structure, be used for injecing the biggest insertion depth and the biggest outer range of drawing of transparent cover 17 respectively, avoid transparent cover 17 to take place to rock or drop.

The working principle of the invention is as follows: firstly, according to the requirements of screening and detecting experiments of biological antibodies, selecting a corresponding biological chip 13, at the moment, slightly pressing the transparent cover plate 17 with force, extruding and deforming the second gasket 18 made of sponge material and the top end of the chip body 1301 to form a recess, so that the bulge 19 can be downwards separated from the first concave hole 15, then sliding and pushing the transparent cover plate 17 outwards until the bulge 19 is embedded and positioned with the second concave hole 16, at the moment, clamping the biological chip 13 from the accommodating cavity 11 by using a special tweezers tool, then placing on an experiment table at the top end of the box body 1 for detection, in the process, an experimenter can carry out accurate positioning through positioning pins 1302 at two sides of the chip body 1301 to ensure that the chip body 1301 is accurately butted with a matched experimental instrument, then dropping a proper amount of reagent into the hollow chip body 1301 by using a titration hole 1303, and after the detection is completed, the biochip 13 can be sealed and stored by reversely operating the above processes, so that the efficiency of taking and placing the biochip 13 is improved;

in the process, the first gasket 12 made of silica gel is elastically adsorbed to the bottom end of the chip body 1301, so that the chip body 1301 is prevented from shaking in the accommodating cavity 11, the second gasket 18 made of sponge is elastically contacted with the top end of the chip body 1301, the chip body 1301 is prevented from being extruded or rubbed and damaged in the sliding and drawing process of the transparent cover plate 17, the two groups of positioning pins 1302 are convenient for accurately positioning the chip body 1301, the placing position is prevented from deviating, and therefore the safety of the biological chip 13 in storage and use is improved;

in addition, after accomplishing a round of testing, the rolling contact between two sets of rubber gyro wheels 7 of accessible and the spout 3, insert the chamber 2 of acceping that corresponds with box body 5 stable insertion, when first magnetism piece 4 and second magnetism piece 8 correspond the actuation, show that box body 5 is accomodate completely promptly, when the biochip 13 of needs use corresponding model, through handle 10 in the last spiral groove 9, be convenient for in time pull out the box body 5 that corresponds hard backward, thereby improved the efficiency of getting of box body 5, and then improved the operating efficiency of detection achievement.

The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention are equivalent to or changed within the technical scope of the present invention.

Claims

1. The utility model provides a quick open-close type antibody screening that facilitates use is with detecting chip, includes box (1), box body (5) and biochip (13), characterized in that, the equidistant a plurality of accepting chamber (2) that is provided with of one side of box (1) inside, and one side of accepting chamber (2) all horizontally extends to the outside of box (1), the inside of accepting chamber (2) all is provided with the box body (5), the inside of box body (5) all is equidistant to be seted up two sets of holding chamber (11), and the inside of holding chamber (11) all is provided with biochip (13), biochip (13) all includes chip body (1301), location pin (1302) and titration hole (1303), all equidistant welding has a plurality of location pin (1302) on two lateral walls of chip body (1301), chip body (1301) all is hollow structure, titration holes (1303) are formed in one side of the top end of the chip body (1301), slots (14) are formed right above the accommodating cavities (11), one sides of the slots (14) extend to the outer portion of the box body (5) horizontally, and transparent cover plates (17) are mounted in the slots (14).

2. The detection chip for screening the convenient-to-use quick-opening and-closing antibody is characterized in that sliding grooves (3) are horizontally formed in two sides of the bottom of the accommodating cavity (2), and one sides of the sliding grooves (3) horizontally extend to the outside of the box body (1).

3. The detection chip for screening the convenient quick open-close type antibody according to claim 1, wherein two first magnetic blocks (4) are symmetrically embedded on one side of the bottom of the accommodating cavity (2), and the surfaces of the first magnetic blocks (4) are flush with the inner side wall of the accommodating cavity (2).

4. The detection chip for screening the rapid open-close type antibody convenient to use according to claim 2, characterized in that a plurality of grooves (6) are formed in two side walls of the bottom of the box body (5) at equal intervals, rubber rollers (7) are rotatably connected to the insides of the grooves (6), one sides of the rubber rollers (7) horizontally extend to the outside of the box body (5), two sets of the rubber rollers (7) are respectively arranged corresponding to the two sets of the sliding grooves (3), and one sides of the rubber rollers (7) are in rolling contact with the inner side walls of the sliding grooves (3).

5. The convenient detection chip for screening the rapid open-close type antibody as claimed in claim 3, wherein two second magnetic attraction blocks (8) are symmetrically embedded on one side of the bottom of the box body (5), and the first magnetic attraction block (4) is arranged corresponding to the second magnetic attraction block (8) and attracted with each other.

6. The detection chip is used in screening of quick open-close type antibody of a facilitating use of claim 1, characterized in that, heavy groove (9) has all been seted up at the middle part of box body (5) one side, and all inlay handle (10) in heavy groove (9), and the both ends of handle (10) all rotate connect in heavy groove (9), heavy groove (9) with handle (10) all are half-circular.

7. The detection chip for screening the convenient and fast open-close type antibody as claimed in claim 1, wherein the central position of the bottom end of the accommodating cavity (11) is attached with a first gasket (12), the first gaskets (12) are made of silica gel, and the top ends of the first gaskets (12) are elastically adsorbed to the bottom end of the chip body (1301).

8. The detection chip for screening the convenient and fast open and close type antibody as claimed in claim 1, wherein two first concave holes (15) are symmetrically formed on one side of the top of the slot (14), and two second concave holes (16) are symmetrically formed on the other side of the top of the slot (14).

9. The detection chip is used in screening of quick open-close type antibody of a claim 1 to facilitate use, characterized in that, transparent cover plate (17) all with slot (14) slip gomphosis and constitute limit structure, transparent cover plate (17) all are toughened glass material, the central point department of transparent cover plate (17) bottom all attaches second gasket (18), and second gasket (18) all are the sponge material, the bottom of second gasket (18) all with the top elastic contact of chip body (1301).

10. The convenient detection chip for screening the quick open-close type antibody according to claim 8, wherein two protrusions (19) are symmetrically arranged on one side of the top end of the transparent cover plate (17), and the protrusions (19) are respectively embedded with the first concave hole (15) and the second concave hole (16) to form a limiting structure.