CN113514644B - Detection chip for rapid on-off type antibody screening convenient to use - Google Patents
Detection chip for rapid on-off type antibody screening convenient to use Download PDFInfo
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- CN113514644B CN113514644B CN202110468447.1A CN202110468447A CN113514644B CN 113514644 B CN113514644 B CN 113514644B CN 202110468447 A CN202110468447 A CN 202110468447A CN 113514644 B CN113514644 B CN 113514644B
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- box body
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6854—Immunoglobulins
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/50—Reuse, recycling or recovery technologies
- Y02W30/60—Glass recycling
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- General Physics & Mathematics (AREA)
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- Automatic Analysis And Handling Materials Therefor (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention relates to the technical field of chip laboratories and discloses a detection chip for rapid opening and closing type antibody screening, which comprises a box body, a box body and a biochip, wherein a plurality of accommodating cavities are formed in one side of the interior of the box body at equal intervals, the box body is arranged in each accommodating cavity, two groups of accommodating cavities are formed in the interior of the box body at equal intervals, the biochip is arranged in each accommodating cavity, the biochip comprises a chip body, positioning pins and titration holes, slots are formed right above the accommodating cavities, and transparent cover plates are arranged in the slots. The invention not only improves the taking and placing efficiency of the biochip, but also adopts the positioning modes of elastic adsorption and elastic contact on the two side surfaces of the biochip, thereby improving the safety of storing and using the biochip, and improving the taking and placing efficiency of the box body with a plurality of groups of biochips.
Description
Technical Field
The invention relates to the technical field of chip laboratories, in particular to a detection chip for rapid on-off antibody screening, which is convenient to use.
Background
The chip laboratory, also called micro total analysis system, is one technology for realizing chemical analysis and detection via analysis chemistry, micro electromechanical processing, computer, electronics, material science, biology, medicine, engineering, etc. For example, when the technology is used for screening and detecting the biological antibody, the related basic operation units such as sample preparation, biological and chemical reactions, separation detection and the like are integrated on a chip with a few square centimeters, so that different biological or chemical reaction processes can be completed at one time, and the products are analyzed, so that the detection efficiency is greatly improved.
In the actual operation process, multiple groups of detection tests are required to be carried out by selecting multiple biological detection chips with different structures according to different experimental purposes and design parameters, in the prior art, the biological detection chips are generally collected and stored through matched kits, but the internal structures of a plurality of kits are complex, so that the biological detection chips are more troublesome to select and take and place, meanwhile, the biological detection chips can shake in the kits to cause corner damage, in addition, the kits and detection instruments are usually stored separately, and the taking and placing process before and after each test is also more complicated.
Therefore, a person skilled in the art provides a detection chip for rapid on-off antibody screening, which is convenient to use, so as to solve the problems in the prior art.
Disclosure of Invention
The invention aims to provide a detection chip for rapid on-off antibody screening, which is convenient to use, so as to solve the problems in the background technology.
In order to achieve the above purpose, the present invention provides the following technical solutions:
the utility model provides a quick start-stop type antibody screening of facilitate use detects chip, includes box, box and biochip, the inside one side equidistant a plurality of chamber of acceping of box, just one side of acceping the chamber all horizontally extending to the outside of box, the inside of acceping the chamber all is provided with the box, two sets of holding chambers have all been offered to the inside equidistant of box, just the inside of holding chamber all is provided with biochip, biochip all includes chip body, location pin and titration orifice, all equidistant welding has a plurality of on the both outer lateral wall of chip body location pin, the chip body all is hollow structure, the titration orifice has all been seted up to one side on chip body top, all seted up the slot directly over the holding chamber, just one side of slot all horizontally extending to the outside of box, transparent cover plate is all installed in the slot.
As still further aspects of the invention: the two sides of the bottom of the containing cavity are horizontally provided with sliding grooves, and one sides of the sliding grooves horizontally extend to the outside of the box body and are used for being mutually matched with a plurality of groups of rubber rollers.
As still further aspects of the invention: two first magnetic blocks are symmetrically inlaid on one side of the bottom of the accommodating cavity, and the surfaces of the first magnetic blocks are flush with the inner side wall of the accommodating cavity, are mutually matched with the second magnetic blocks, and are prevented from colliding with the box body.
As still further aspects of the invention: a plurality of grooves are formed in the two side walls of the bottom of the box body at equal intervals, rubber rollers are connected to the inner portions of the grooves in a rotating mode, one sides of the rubber rollers horizontally extend to the outer portion of the box body, two groups of the rubber rollers are respectively arranged corresponding to the two groups of sliding grooves, one sides of the rubber rollers are in rolling contact with the inner side walls of the sliding grooves, the sliding grooves are used for assisting in limiting the translation track of the box body, contact friction is reduced, and therefore the box body is prevented from being deviated or blocked in the drawing process.
As still further aspects of the invention: two second magnetic attraction blocks are symmetrically inlaid on one side of the bottom of the box body, and the first magnetic attraction blocks are arranged corresponding to the second magnetic attraction blocks and are mutually attracted and used for automatically locking the box body after being placed in place, so that relative sealing is maintained, and falling caused by self-moving of the box body is avoided.
As still further aspects of the invention: the middle part of box body one side has all seted up heavy groove, just all inlay in the heavy groove has the handle, and the both ends of handle all rotate connect in the heavy groove, heavy groove with the handle all is semicircle annular, is convenient for manual the taking out and placing the box body, and reduce unnecessary space occupation.
As still further aspects of the invention: the first gaskets are adhered to the central positions of the bottom ends of the accommodating cavities, the first gaskets are made of silica gel, and the top ends of the first gaskets are elastically adsorbed to the bottom ends of the chip bodies so as to prevent the chip bodies from being displaced or damaged by collision in the accommodating cavities.
As still further aspects of the invention: two first concave holes are symmetrically formed in one side of the top of the slot, and two second concave holes are symmetrically formed in the other side of the top of the slot and are used for being mutually matched with the protrusions.
As still further aspects of the invention: the transparent cover plate is in sliding fit with the slot and forms a limiting structure, the transparent cover plate is made of toughened glass, a second gasket is attached to the central position of the bottom end of the transparent cover plate, the second gasket is made of sponge, the bottom end of the second gasket is in elastic contact with the top end of the chip body, a certain elastic shrinkage space is provided, and the contact surface is kept flexible during sliding contact, so that the chip body is prevented from being extruded or damaged by friction.
As still further aspects of the invention: two bulges are symmetrically arranged on one side of the top end of the transparent cover plate, and are respectively mutually embedded with the first concave holes and the second concave holes to form a limiting structure, and the limiting structure is respectively used for limiting the maximum insertion depth and the maximum pull-out amplitude of the transparent cover plate so as to prevent the transparent cover plate from shaking or falling.
Compared with the prior art, the invention has the beneficial effects that:
1. the transparent cover plate is pressed with a little force, so that the bulge is separated from the first concave hole, and then the transparent cover plate is slid outwards until the bulge is embedded with the second concave hole for positioning, at the moment, the biochip can be clamped out from the accommodating cavity and then placed on the experiment table at the top end of the box body for detection, and after detection is finished, the biochip can be sealed by reversing the process, so that the taking and placing efficiency of the biochip is improved;
2. the first gasket made of silica gel material is elastically adsorbed with the bottom end of the chip body, so that the chip body is prevented from shaking in the accommodating cavity, the second gasket made of sponge material is elastically contacted with the top end of the chip body, the chip body is prevented from being extruded or damaged by friction, and the two groups of positioning pins are convenient for accurately positioning the chip body, and the placing position is prevented from being deviated, so that the safety of storing and using the biochip is improved;
3. through the rolling contact between two sets of rubber gyro wheels and the spout, be convenient for insert the box body in acceping the chamber steadily, when first magnetism is inhaled the piece and is inhaled the piece with the second magnetism corresponds, indicate that the box body is accomodate completely promptly, or through the handle in the countersink groove that revolves, can pull out the box body with reverse power to the efficiency of putting is got to the box body has been improved.
Drawings
FIG. 1 is a schematic perspective view of a detection chip for rapid on-off antibody screening, which is convenient to use;
FIG. 2 is an enlarged schematic view of the structure of the portion A of FIG. 1 in a detection chip for rapid on-off antibody screening, which is convenient to use;
FIG. 3 is a schematic diagram showing the development of a cartridge in a detection chip for rapid on-off antibody screening, which is convenient to use;
FIG. 4 is a schematic view of a part of the structure of a cartridge in a detection chip for rapid on-off antibody screening, which is convenient to use;
FIG. 5 is a schematic diagram of an explosion structure of a receiving cavity in a detection chip for rapid on-off antibody screening, which is convenient to use;
FIG. 6 is an enlarged schematic view of a biochip in a conveniently used detection chip for rapid on-off antibody screening.
In the figure: 1. a case; 2. a housing chamber; 3. a chute; 4. a first magnetic block; 5. a case body; 6. a groove; 7. rubber rollers; 8. a second magnetic block; 9. sinking grooves; 10. a handle; 11. a receiving chamber; 12. a first gasket; 13. a biochip; 1301. a chip body; 1302. positioning pins; 1303. titration wells; 14. a slot; 15. a first concave hole; 16. a second concave hole; 17. a transparent cover plate; 18. a second gasket; 19. a protrusion.
Detailed Description
Referring to fig. 1-6, in an embodiment of the invention, a detection chip for rapid on-off antibody screening, which is convenient to use, comprises a box 1, a box 5 and a biochip 13, wherein a plurality of accommodating cavities 2 are arranged at equal intervals on one side of the inside of the box 1, one sides of the accommodating cavities 2 horizontally extend to the outside of the box 1, the inside of the accommodating cavities 2 is provided with the box 5, two groups of accommodating cavities 11 are arranged at equal intervals in the inside of the box 5, the inside of the accommodating cavities 11 is provided with the biochip 13, the biochip 13 comprises a chip body 1301, positioning pins 1302 and titration holes 1303, a plurality of positioning pins 1302 are welded on two outer side walls of the chip body 1301 at equal intervals, the chip body 1301 is of a hollow structure, the titration holes 1303 are arranged on one side of the top end of the chip body 1301, slots 14 are arranged above the accommodating cavities 11, one sides of the slots 14 horizontally extend to the outside of the box 5, and transparent cover plates 17 are arranged in the slots 14.
In fig. 1, 2, 3 and 4: the two sides of the bottom of the accommodating cavity 2 are horizontally provided with sliding grooves 3, and one side of each sliding groove 3 horizontally extends to the outside of the box body 1 and is used for being mutually matched with a plurality of groups of rubber rollers 7; two first magnetic blocks 4 are symmetrically embedded on one side of the bottom of the accommodating cavity 2, and the surfaces of the first magnetic blocks 4 are flush with the inner side wall of the accommodating cavity 2 and are used for being mutually matched with the second magnetic blocks 8 and avoiding collision with the box body 5; a plurality of grooves 6 are formed in the two side walls of the bottom of the box body 5 at equal intervals, rubber rollers 7 are rotatably connected to the inside of the grooves 6, one sides of the rubber rollers 7 horizontally extend to the outside of the box body 5, two groups of rubber rollers 7 are respectively arranged corresponding to the two groups of sliding grooves 3, one sides of the rubber rollers 7 are in rolling contact with the inner side walls of the sliding grooves 3 and are used for assisting in limiting the translation track of the box body 5 and reducing contact friction, so that the box body 5 is prevented from shifting or clamping in the drawing process; two second magnetic attraction blocks 8 are symmetrically inlaid on one side of the bottom of the box body 5, and the first magnetic attraction blocks 4 are arranged corresponding to the second magnetic attraction blocks 8 and are attracted mutually and used for automatically locking the box body 5 after being placed in place, so that relative sealing is maintained, and falling caused by self-moving of the box body 5 is avoided; the middle part of one side of the box body 5 is provided with a sinking groove 9, a lifting handle 10 is inlaid in the sinking groove 9, two ends of the lifting handle 10 are rotatably connected in the sinking groove 9, and the sinking groove 9 and the lifting handle 10 are semi-circular, so that the box body 5 can be conveniently pulled out and placed manually, and unnecessary space occupation is reduced.
In fig. 2, 3 and 5: the first gaskets 12 are attached to the central positions of the bottom ends of the accommodating cavities 11, the first gaskets 12 are made of silica gel, and the top ends of the first gaskets 12 are elastically adsorbed to the bottom ends of the chip bodies 1301, so that the chip bodies 1301 are prevented from being displaced or damaged by collision in the accommodating cavities 11; the transparent cover plate 17 is slidably embedded with the slot 14 and forms a limiting structure, the transparent cover plate 17 is made of toughened glass, the second gasket 18 is attached to the central position of the bottom end of the transparent cover plate 17, the second gasket 18 is made of sponge, the bottom end of the second gasket 18 is elastically contacted with the top end of the chip body 1301, a certain elastic shrinkage space is provided, and the contact surface is kept flexible during sliding contact, so that the chip body 1301 is prevented from being extruded or friction damaged.
In fig. 3 and 5: two first concave holes 15 are symmetrically formed on one side of the top of the slot 14, and two second concave holes 16 are symmetrically formed on the other side of the top of the slot 14 and are used for being mutually matched with the protrusions 19; two bulges 19 are symmetrically arranged on one side of the top end of the transparent cover plate 17, and the bulges 19 are respectively embedded with the first concave holes 15 and the second concave holes 16 to form a limiting structure, and are respectively used for limiting the maximum insertion depth and the maximum pull-out amplitude of the transparent cover plate 17 so as to prevent the transparent cover plate 17 from shaking or falling.
The working principle of the invention is as follows: firstly, selecting a corresponding biochip 13 according to the requirement of a biological antibody screening test, at this time, pressing the transparent cover plate 17 with a little force, extruding and deforming the second gasket 18 made of sponge material with the top end of the chip body 1301 to be concave, so that the bulge 19 can be separated from the first concave hole 15 downwards, sliding and pushing the transparent cover plate 17 outwards until the bulge 19 is embedded with the second concave hole 16, at this time, clamping the biochip 13 from the accommodating cavity 11 by using a special tweezers tool, and then placing the biochip 13 on a test table at the top end of the box body 1 for test, in the process, an experimenter can accurately position the positioning pins 1302 at two sides of the chip body 1301 to ensure that the chip body 1301 is accurately abutted with a matched experimental instrument, then dripping a proper amount of reagent into the hollow structure chip body 1301 by using the titration hole 1303 to start the test, and after the test is completed, sealing the biochip 13 can be stored by reversing the process, thereby improving the taking and placing efficiency of the biochip 13;
in the above process, the first pad 12 made of silica gel material elastically adsorbs the bottom end of the chip body 1301, so as to avoid the chip body 1301 from shaking in the accommodating cavity 11, and the second pad 18 made of sponge material elastically contacts the top end of the chip body 1301, so as to avoid the chip body 1301 from being extruded or friction damaged in the sliding and drawing process of the transparent cover plate 17, and the two groups of positioning pins 1302 are convenient for accurately positioning the chip body 1301, so that the placement position is prevented from being deviated, and the safety of the biochip 13 during storage and use is improved;
in addition, after finishing a round of detection test, the rolling contact between two sets of rubber gyro wheels 7 and spout 3, insert box body 5 steadily in corresponding acceping chamber 2, when first magnetism inhale piece 4 and second magnetism inhale piece 8 correspond the suction, indicate that box body 5 is accomodate completely promptly, when the biochip 13 of corresponding model needs to be used, through the handle 10 in the supination sink 9, be convenient for in time draw out corresponding box body 5 reverse power to the efficiency of putting of getting to box body 5 has been improved, and then the operating efficiency of detection work has been improved.
The foregoing description is only a preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art, who is within the scope of the present invention, should make equivalent substitutions or modifications according to the technical solution of the present invention and the inventive concept thereof, and should be covered by the scope of the present invention.
Claims (4)
1. The utility model provides a detection chip for quick start-stop antibody screening that facilitates use, includes box (1), box (5) and biochip (13), its characterized in that, one side of box (1) inside equidistant is provided with a plurality of holding chamber (2), just hold one side of chamber (2) all horizontally extends to the outside of box (1), hold the inside of chamber (2) all to be provided with box (5), two sets of holding chamber (11) have all been offered to the inside equidistant of box (5), just hold the inside of chamber (11) all to be provided with biochip (13), biochip (13) all include chip body (1301), location pin (1302) and titration hole (1303), all equidistant welding has a plurality of on the both outer lateral walls of chip body (1301) location pin (1302), chip body (1301) all are hollow structure, one side on the top of chip body (1301) all has seted up accommodation hole (1303), just top cavity (11) all has been provided with biochip (13), just all to the slot (14) have been seted up in the slot (14) all to the slot (14) have extended in the slot (14);
a sliding groove (3) is horizontally formed in two sides of the bottom of the accommodating cavity (2), and one side of the sliding groove (3) horizontally extends to the outside of the box body (1);
a plurality of grooves (6) are formed in the two side walls of the bottom of the box body (5) at equal intervals, rubber rollers (7) are rotatably connected in the grooves (6), one sides of the rubber rollers (7) horizontally extend to the outside of the box body (5), two groups of the rubber rollers (7) are respectively arranged corresponding to the two groups of sliding grooves (3), and one sides of the rubber rollers (7) are in rolling contact with the inner side walls of the sliding grooves (3);
a first gasket (12) is attached to the central position of the bottom end of the accommodating cavity (11), the first gaskets (12) are made of silica gel, and the top ends of the first gaskets (12) are elastically adsorbed to the bottom end of the chip body (1301);
two first concave holes (15) are symmetrically formed in one side of the top of the slot (14), and two second concave holes (16) are symmetrically formed in the other side of the top of the slot (14);
the transparent cover plates (17) are all in sliding fit with the slots (14) and form a limiting structure, the transparent cover plates (17) are made of toughened glass, second gaskets (18) are attached to the central positions of the bottom ends of the transparent cover plates (17), the second gaskets (18) are made of sponge materials, and the bottom ends of the second gaskets (18) are in elastic contact with the top ends of the chip bodies (1301);
two bulges (19) are symmetrically arranged on one side of the top end of the transparent cover plate (17), and the bulges (19) are respectively embedded with the first concave holes (15) and the second concave holes (16) to form a limiting structure.
2. The detection chip for rapid on-off antibody screening, which is convenient to use, according to claim 1, wherein two first magnetic blocks (4) are symmetrically embedded on one side of the bottom of the accommodating cavity (2), and the surfaces of the first magnetic blocks (4) are flush with the inner side wall of the accommodating cavity (2).
3. The detection chip for rapid on-off antibody screening, which is convenient to use, according to claim 2, wherein two second magnetic blocks (8) are symmetrically embedded on one side of the bottom of the box body (5), and the first magnetic blocks (4) are arranged corresponding to the second magnetic blocks (8) and are attracted with each other.
4. The detection chip for rapid on-off antibody screening, which is convenient to use, according to claim 3, wherein a sinking groove (9) is formed in the middle of one side of the box body (5), a lifting handle (10) is embedded in the sinking groove (9), two ends of the lifting handle (10) are rotatably connected in the sinking groove (9), and the sinking groove (9) and the lifting handle (10) are semicircular.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110468447.1A CN113514644B (en) | 2021-04-28 | 2021-04-28 | Detection chip for rapid on-off type antibody screening convenient to use |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110468447.1A CN113514644B (en) | 2021-04-28 | 2021-04-28 | Detection chip for rapid on-off type antibody screening convenient to use |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN113514644A CN113514644A (en) | 2021-10-19 |
| CN113514644B true CN113514644B (en) | 2023-07-25 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202110468447.1A Active CN113514644B (en) | 2021-04-28 | 2021-04-28 | Detection chip for rapid on-off type antibody screening convenient to use |
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| CN (1) | CN113514644B (en) |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2280497C (en) * | 1999-08-19 | 2003-12-09 | Andre Lafond | Biological specimen cassette |
| EP1161984A1 (en) * | 2000-06-08 | 2001-12-12 | F. Hoffmann-La Roche Ag | Device for packaging a chip shaped carrier and process for assembling a plurality of such carriers |
| CN204958886U (en) * | 2015-06-10 | 2016-01-13 | 张璐华 | Biochip reaction box |
| CN205199530U (en) * | 2015-11-24 | 2016-05-04 | 北京市农林科学院 | Leek storage box that incomplete testing process of leek farming was used |
| CN105749995B (en) * | 2016-04-28 | 2017-10-31 | 宁波大学 | A kind of biochip hybridizing device |
| CN106754244A (en) * | 2016-12-05 | 2017-05-31 | 成都叮当自动化设备有限公司 | A kind of genetic chip case |
| CN111890318A (en) * | 2019-05-06 | 2020-11-06 | 江苏康正生物科技有限公司 | Antibody screening that facilitates use is with detecting chip |
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2021
- 2021-04-28 CN CN202110468447.1A patent/CN113514644B/en active Active
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| CN113514644A (en) | 2021-10-19 |
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