CN105746520B - Application of the nanometer chitin in terms of tobacco production and quality is improved - Google Patents
Application of the nanometer chitin in terms of tobacco production and quality is improved Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
- A01N25/04—Dispersions, emulsions, suspoemulsions, suspension concentrates or gels
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
- A01N37/46—N-acyl derivatives
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
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- A01N47/28—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N<
- A01N47/34—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N< containing the groups, e.g. biuret; Thio analogues thereof; Urea-aldehyde condensation products
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
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Abstract
The present invention prepares a kind of novel nano biomaterial nanometer chitin through acidolysis deacylation.This nanometer of chitin aqueous suspension agent is the nanometer chitin whisker particle with polycation property, and the average-size of its whisker is length 100-150nm, width 15-30nm, comprehensive surface band lotus amount 33mmol/kg.This nanometer of chitin water slurry processing tobacco seed can improve the germinating energy of seed, processing seedbed phase tobacco seedling and can improve the growing way of seedling, the thick plant height of tobacco, stem, the number of blade and maximum leaf area, its Agronomic trait to raising tobacco mixed with thiophanate-methyl can be improved to the tobacco seedlings pouring root after transplanting there is good Synergistic and subtracting for chemical pesticide to apply use；After its processing mixed with metalaxyl-mn-zn is transplanted, the preventive effect to tobacco root rot can be improved, the consumption of chemical bactericide is reduced.
Description
Technical field
The invention belongs to technical field of tobacco, and in particular to a kind of new bio nano material nanometer chitin is given birth in tobacco
The application of application in production, especially nanometer chitin in terms of tobacco production and quality is improved.
Background technology
Bio-Nano-Materials are acknowledged as before 21 century most promising scientific research field, International Biotechnology field
Edge and hot-topic subject, nanometer technology are triggering a new industrial technology revolution.Nanosecond science and technology with it is agrotechnical mutually
Infiltration, has also expedited the emergence of the new branch of science such as agriculture nanosecond science and technology, and new theoretical, methods and techniques means are provided for agricultural sciences, must
The development of the future of agriculture will be produced extensively and far-reaching influence.Nano material has the big effect of special four：Skin effect, body
Product effect, quantum effect and macro quanta tunnel effect.These special effects make nano material that there is traditional material not have
Standby unusual or unusual Wuli-Shili-Renli system approach.
Chitin（Chitin, structural formula is as shown in bottom left）It is the renewable polymer for being widely present in nature, mainly
Source be the shell of shellfish such as shrimp, crab, insect and molluscan organ（The cartilage of such as cuttlefish）, and fungi
Cell membrane of class etc..Second is accounted in the natural polymer of its reserves on earth, the synthesis of annual nature biotechnology is estimated
Amount is up to 1 × 1011Ton, is only second to cellulose.Under natural conditions, chitin is water insoluble, is also insoluble in sour or organic molten
Agent, therefore be difficult to be used effectively.Chitosan（Chitosan, structural formula is as shown in bottom right）It is the derivative of chitin, in spy
Under the conditions of different, chitin is degraded and de-acyl reaction is obtained.Chitosan is soluble in weak acid.In weak acid
In, positively charged polymer is become after the protonated amino on chitosan, so as to bioactivity, be widely used
In fields such as medicine, plant pest managements.
As cellulose, natural chitin is also to exist with filamentous form, passes through intermolecular and intramolecular hydrogen bond
N- acetyl glucosamine molecule in close is combined together, the chitin fiber with crystal and noncrystal two kinds of structures is formed.It is several
The crystal structure of fourth matter has three kinds, i.e. α, β and γ types.The chitin of separate sources, its chemical constitution is different with property.Outside shrimp shell
Shell is mainly α-type, and the gladius of squid is mainly β-type, and the chitin of fungal cell wall is mainly γ-type（Crystallinity compared with
It is low）.α-type, β-type chitin is most abundant, and crystallinity is high, most stable, is difficult fusing, not readily dissolves, while being not easy to be dissolved in
Common organic solvents.α-type chitin resource is most abundant, it is easy to industrialized production.
The manufacture craft of pure chitin is fairly simple, and the method commonly used at present in production is by raw material（Shrimp shell or crab shell）
Chitin is obtained by de- albumen, desalination, depigmentation, bleaching, drying grinding.Conventionally produced chitosan is that chemical deacylation is anti-
Answer or Hydrolyze method, its principle is the amorphous area by hydrolyzing destruction chitin, makes part N- in molecular scission, strand
Acetyl glucosamine deacylation, improves hydrophily.But chemical deacylation or Hydrolyze method reaction condition require higher, should not control, and instead
It is longer between seasonable, power consumption, environmental pollution.These problems can be solved using enzymatic isolation method, including the use of chitinase, chitosan enzyme
It is hydrolyzed with lysozyme.But this fermentoid is expensive and is difficult to obtain, and causes product cost too high.
Hydrochloric acid hydrolysis is that conventional method prepares nanometer chitin.α-type chitin can produce size through sour water solution
150-800nm length, wide 5-70nm chitin nano particle.The present invention is to use 3M HCl, by 1:300 ratio is in 85-
90oHydrolyzed 2 hours under the conditions of C.Its product is 100-150nm length through dialysis, ultrasonically treated acquisition granularity, and width 15-30nm is wide
Chitin nano whisker, comprehensive surface charge density is 33mmol/kg.
Due to the natural property and unique molecular structure of chitin, increasingly paid attention to by scientific circles.Numerous studies
Show, chitin is in addition to performances such as nontoxic, bio-compatible, biodegradabilities, the derivative of chitin（Chitosan）
It can be used as the prevention and control of plant diseases, pest control, seed pelleting, the coating of fruits and vegetables in agricultural production, plant growth can also be improved, lured
Send out plant resistance to environment stress and improve the effect of via plant immunity.Nanometer chitin also has very in addition to inheriting the fundamental property of chitin
The property of high specific surface area, high-crystallinity and polycation, is considered as the natural macromolecular material of excellent combination property.Utilize
The nanometer chitin prepared is hydrolyzed, specific surface area is larger and carries a certain amount of polycation, and its nano particle is more poly- than general shell
Sugar has more significant bioactivity.Detected through toxicity, 0.3% nanometer of chitin aqueous suspension agent is to male and the urgency of female rats
Property footpath mouthful LD50 be all higher than 5000mg/kg.BW, acute percutaneous LD50 is all higher than 2000mg/kg.BW, basic to other biological
It is nontoxic.There are some researches show nano titanium oxide, multilayer carbon nanotube, metal nanoparticle and receiving based on nano material
Rice inductor etc., these materials are agriculturally having application, but have the nanometer of good biological characteristic and application value several
Application of the fourth matter on tobacco be not also by system research.
Tobacco is the important industrial crops of China, and pest and disease damage is to influence the key factor of China's tobacco production and quality.
Improvement to these pest and disease damages, chemical prevention is one of major measure for using in production, but extensively, it is a large amount of and be used for a long time
Chemical pesticide causes agricultural chemical residue in tobacco increase, the once again resistance to the action of a drug enhancing of disease pest and wildness, i.e. " three R " problem.Study table
It is bright, there are the nearly 70 kinds chemical substances that may interfere with human endocrine at present, its Pesticides just there are more than 40 kinds.In recent years due to using
The food such as the residual exceeded veterinary antibiotics of agriculture and cause the event of pesticide poisoning of common occurrence.Although tobacco can not directly be eaten,
But tobacco is still divided into foodstuff or quasi- foodstuff by many countries, as product are sucked, tobacco occurs greatly in combustion
Complicated chemical reaction is measured, many new chemical substances are produced, the agricultural chemicals of residual can also participate.Residues of pesticides index into
For the important content in various countries tobacco and tobacco product quality control.Therefore modern agriculture is in the urgent need to efficient, environment-friendly
New formulation replaces or reduced the use of chemistry and fertilizer.
The content of the invention
Present invention aims to overcome that prior art defect is improving tobacco production and quality there is provided a kind of nanometer chitin
The application of aspect.
To achieve the above object, the present invention is adopted the following technical scheme that：
Application of the nanometer chitin in terms of tobacco production and quality is improved.
Specifically, can be with 0.001-0.02% nanometers of chitin water slurry processing tobacco seeds, to improve tobacco seed
The germinating energy of son.
Specifically, can be with 0.001-0.01% nanometers of chitin water slurry processing seedbed phase tobacco seedlings, to improve
The growing way of tobacco seedling.
Specifically, can be filled with 0.001-0.005% nanometers of chitin water slurries to the tobacco seedling after transplanting
Root, to improve the thick plant height of tobacco, stem, the number of blade and leaf area.
Specifically, can be by 0.001-0.005% nanometers of chitin water slurries and the mixed processing of 36% thiophanate-methyl
Tobacco seedling after transplanting, to improve the Agronomic trait of tobacco, such as plant height, stem are thick, the number of blade and leaf area, while can be with
Reduce the applied amount of chemical pesticide.
Specifically, the cigarette after can the processing mixed with metalaxyl-mn-zn of 0.002% nanometer of chitin water slurry be transplanted
Grass, to improve the preventive effect to tobacco root rot, while reducing the consumption of chemical bactericide.
The present invention improves the yield and quality of tobacco using new bio nano material nanometer chitin, and utilizes it
Tobacco rhizome disease is prevented and treated with the synergistic function of common fungicide, thus solve it is a large amount of using chemicals, agricultural chemicals,
Chemical fertilizer etc. has resulted in the great societies such as the increase of pest and disease damage resistance, soil degradation, environmental pollution, Practice for Pesticide Residue in Agricultural Products be exceeded and asked
Topic.
Compared to the prior art, beneficial effects of the present invention：
1）Nanometer chitin of the present invention is a kind of environmentally friendly nanometer formulation-water slurry, without any organic or
Inorganic assistant；2）Ultralow consumption application can improve the Agronomic trait of crop, increasing yield and improving quality on tobacco, and can reduce to soil
The influence of earth, water and environment；3）It is mixed with common fungicide to improve protection effect, while the consumption of bactericide is reduced,
Residues of pesticides are reduced, the destruction and pollution to human and environment is greatly reduced；4）Technical requirements to user are not high, and can save
When, it is saving of labor, laborsaving, meet Rural Areas at Present social economy reality；5）With offer nutrient, improved soil and controlling crop diseases and insect pests
A variety of functions；6）It is main to produce nano material using agricultural wastes, recycling for agricultural wastes resource can be promoted.
Brief description of the drawings
Fig. 1 is the facilitation that nanometer chitin water slurry grows to seedbed phase tobacco seedling；
Fig. 2 is influence of the nanometer chitin water slurry to tobacco plant character；
Fig. 3 is nanometer chitin water slurry and the mixed Synergistic to tobacco plant character of 36% thiophanate-methyl
Effect；
Fig. 4 is that the nanometer chitin water slurry Synergistic to preventing and treating tobacco diseases mixed with metalaxyl-mn-zn is made
With；
Fig. 5 is the transmission electron microscope picture of nanometer chitin of the present invention, and it can be seen from the figure that, chitin nano particle is whisker knot
Structure, granularity is in 100-150nm length, and 15-30nm is wide.
Embodiment
Technical scheme is further discussed in detail with reference to embodiments, but protection scope of the present invention
It is not limited thereto.
In following embodiments, nanometer chitin used is obtained through following method：
The present invention uses 3M HCl, by raw material chitin（Sigma-Aldrich, C9213-1KG）With the weight of 3M hydrochloric acid
Volume ratio 1g：300mL mixes both, is hydrolyzed 2 hours under the conditions of 85-90 DEG C.After hydrolysis terminates, product regenerated fiber
Plain dialysis membrane（MWCF12-14KDa）Dialysed in deionized water under normal temperature, it is steady to the pH separated out in liquid to discharge free acid
It is fixed.Product after dialysis is ultrasonically treated 3-10 minutes under the conditions of 0 DEG C, then under the conditions of 4 DEG C at a high speed（12000rpm）From
The heart 15 minutes, is removed after precipitation, that is, it is 100-150nm length, chitin nano whisker water wide width 15-30nm to obtain granularity
Suspension（See Fig. 5）, the synthesis surface charge density of its nano-particle is 33mmol/kg.Determined through acute toxicology, nanometer chitin
Matter aqueous suspensions are all higher than 5000mg/Kg.BW to the acute footpath mouthful LD50 of female and male rat, and acute percutaneous LD50 is more than
2000mg/Kg.BW.Therefore novel tobacco adjuvant can be used as.
By the nanometer chitin obtained by above-mentioned preparation be configured to mass concentration 0.002%, 0.004%, 0.006%, 0.008%,
0.01% nanometer chitin water slurry carries out following application tests.
Following embodiments carry out correlation test by taking tobacco bred Zhongyan-100 as an example.
1 nanometer of chitin processing tobacco seed of embodiment improves potentiality of seed
Indoor 0.002-0.01% nanometer chitin water slurry culture tobacco seed, compares with clear water control, receives
Rice chitin can significantly improve Tobacco Seed Germination gesture（P<0.05）（Table 1）.Specific method：Indoor selection is substantial, full
Seed is placed in centrifuge tube, rear several times standby with distillation washing.Tobacco seed is handled with nanometer chitin, sprouting for seed is observed
Start state.It is respectively that isometric 0.002%, 0.004%, 0.006%, 0.008%, 0.01% (wt/v) nanometer chitin water is outstanding
Supernatant liquid is added in the culture dish for being lined with two layers of filter paper.Experiment is compared with deionized water.Each 3 repetitions of processing, Mei Gechong
Multiple 100 tobacco seeds.Culture dish is put into PQX type multi-segment Programmable growth cabinets（Temperature is 27 DEG C, and relative humidity is
60%, light application time 12h）, the nanometer chitin suspension of corresponding equivalent is supplemented every 24h.Nanometer chitin is to tobacco seed
The investigation method of germinating energy influence：The germination of observation seed is recorded every 12h, reaches seed length as germination using radicle.
Germinating energy is defined as：Germination most same day and the seed amount of all germinations before.
Table 1：Influence of the nanometer chitin to Tobacco Seed Germination gesture
Note：Capitalization English letter represents the significance level of difference, P in table<0.01；Small English alphabet represents significant difference
Level, P<0.05.
2 nanometers of chitins of embodiment improve the growing way of seedbed phase tobacco seedling
Indoor 0.001-0.01% nanometers of chitin handles seedbed phase tobacco seedling, is compared with clear water control, nanometer is several
The growth of seedling of fourth confrontation seedbed phase has facilitation（See Fig. 1）, different processing method is different to the growth effect of seedling.Tool
Body method：Sprayed by whole strain and apply two ways with root, respectively with 0.001%, 0.003%, 0.005%, 0.01% nanometer of chitin
The consistent tobacco seedling of seedbed phase growing state is handled, the growing way of seedling is observed after 30 days.Spray standard：Blade face drop is uniformly satisfied
With untill, every plant of 3-4ml：Root applies standard：Per cave 10ml.Each 5 repetitions of processing.
Influence of the 3 nanometers of chitins of embodiment to tobacco plant height, thick stem, the number of blade and leaf area
Under the natural conditions of field, respectively with 0.001%, 0.005% nanometer chitin water slurry to the cigarette after transplanting
Careless seedling carries out pouring root（100mL/ plants）, managed and topdressed by normal tobacco growing.Result of the test is shown in Table 2 and Fig. 2.
Tobacco after test process is higher by more than 16% at plant height, the thick aspect of stem than blank control, and leaf area is higher than compareing
The nanometer chitin for going out more than 33.76%, and 0.001% promotes nanometer chitin of the effect than 0.005% of plant growth more preferable
（Fig. 2）.Tobacco after being handled through spss software analysis reaches in plant height, thick stem, the number of blade and leaf area with control
Significant difference（Table 2）.
Table 2：Influence result of the nanometer chitin to tobacco plant character
Note：Capitalization English letter represents the significance level of difference, P in table<0.01；Small English alphabet represents significant difference
Level, P<0.05.
4 nanometers of chitins of embodiment and the mixed synergistic function to tobacco plant character of 36% thiophanate-methyl
Under field conditions (factors), constant bactericide is used respectively（36% 800 times of thiophanate-methyl）, 0.001%, 0.005% nanometer it is several
Tobacco after fourth matter water slurry and nanometer chitin and constant, decrement 25%, the transplanting of the bactericide mixed processing of decrement 50%
Seedling, field is managed and topdressed by normal tobacco growing, and result of the test is shown in Table 3 and Fig. 3.
Found through experiment：Compared with blank control, 0.001-0.005% nanometers of chitins and bactericide（Constant, decrement
25%th, decrement 50%）The tobacco of mixed processing has reached significant difference in terms of plant height, thick stem, the number of blade and leaf area（P<
0.05）.
Table 3：The synergistic function result of nanometer chitin and bactericide
Remarks：T-0-0：Blank control；T-0-1：36% thiophanate-methyl；T-1-0：0.001% nanometer of chitin aqueous suspension
Liquid；T-1-1：0.001% nanometer of chitin water slurry and 36% thiophanate-methyl constant are mixed；T-1-2：0.001% nanometer of chitin
Matter water slurry and 36% thiophanate-methyl decrement 25% are mixed；T-1-3：0.001% nanometer of chitin water slurry and 36% methyl
Thiophanate decrement 50% is used with；T-5-0：0.005% nanometer of chitin water slurry；T-5-1：0.005% nanometer of chitin aqueous suspension
Liquid and 36% thiophanate-methyl constant are mixed；T-5-2：0.005% nanometer of chitin water slurry and 36% thiophanate-methyl decrement
25% uses with；T-5-3：0.005% nanometer of chitin water slurry and 36% thiophanate-methyl decrement 50% are mixed
5 nanometers of chitins of embodiment synergistic function to preventing and treating tobacco diseases mixed with metalaxyl-mn-zn
Under field conditions (factors), respectively with 0.002% nanometer of chitin water slurry, bactericide routine administration amount（Metalaxyl
MnZn, 1.5 kilograms/hectare）And nanometer chitin water slurry is mixed with the metalaxyl-mn-zn of decrement 25%, decrement 50%
The tobacco after transplanting 70 days is handled, field is managed and topdressed by normal tobacco growing.Result of the test is shown in Table 4 and Fig. 4.
Found through experiment：Nanometer chitin is to the obvious synergy of the white MnZn of first, metalaxyl-mn-zn routine
Dosage prevention effect mixed with nanometer chitin is 62.57%, is single use the 1.13 of metalaxyl-mn-zn prevention effect
Times；The prevention effect mixed with nanometer chitin of metalaxyl-mn-zn decrement 25% is 56.45%, is imitated with the white MnZn preventing and treating of alone first
Significant difference is not present between fruit；Nanometer chitin, which is used alone, also there is certain preventing and treating to make fusarium tabacinum root rot
With preventive effect is 24.26%；The prevention effect pole mixed with nanometer chitin of first frost MnZn decrement 50% is remarkably decreased, and preventive effect is
14.07%（Table 4）.
Table 4：Nanometer chitin and bactericide to prevent and treat tobacco root rot synergistic function result
Remarks：CK：72% first frost MnZn routine administration amount；Processing two：72% first frost MnZn routine administration amount+nanometer is several
Ding Zhi；Processing three：The 75%+ nanometer chitins of 72% first frost MnZn routine administration amount；Processing four：72% first frost MnZn is conventional
The 50%+ nanometer chitins of dosage；Processing one：Nanometer chitin；CK0：Clear water is compareed.
It in summary it can be seen：Nanometer chitin biomaterial of the present invention can promote tobacco growing, controlling disease, reductionization
Learn to farm the consumption of medicine, reduction agricultural chemicals use the pollution to environment.If by nano material antibacterial growth promotion and improvement
The characteristics of environment, is cementing or coating slow/controlled release bactericide is combined with nano material, develops multifunctional nano bactericide, can
To reduce the administration of chemical pesticide and growth regulator, reduce soil and it is adsorbed, mitigate the pollution of soil and groundwater, change
Kind soil physical chemistry structure, improves the utilization rate of soil and crop to nutrient.
Claims (6)
1. application of nanometer chitin in terms of tobacco production and quality is improved；The nanometer chitin is granularity in 100-
150nm length, 15-30nm wide chitin nano whisker.
2. application of the as claimed in claim 1 nanometer of chitin in terms of tobacco production and quality is improved, it is characterised in that use
0.001-0.02% nanometers of chitin water slurries handle tobacco seeds to improve the germinating energy of tobacco seed.
3. application of the as claimed in claim 1 nanometer of chitin in terms of tobacco production and quality is improved, it is characterised in that use
0.001-0.01% nanometers of chitin water slurries handle seedbed phase tobacco seedlings to improve the growing way of tobacco seedling.
4. application of the as claimed in claim 1 nanometer of chitin in terms of tobacco production and quality is improved, it is characterised in that use
0.001-0.005% nanometers of chitin water slurries carry out pouring root to improve plant height, the stem of tobacco to the tobacco seedling after transplanting
Slightly, the number of blade and leaf area.
5. application of the as claimed in claim 1 nanometer of chitin in terms of tobacco production and quality is improved, it is characterised in that
0.001-0.005% nanometers of chitin water slurries and 36% thiophanate-methyl use the tobacco seedling after processing is transplanted with to improve
The plant height of tobacco, stem are thick, the number of blade and leaf area.
6. application of the as claimed in claim 1 nanometer of chitin in terms of tobacco production and quality is improved, it is characterised in that
0.002% nanometer of chitin water slurry is mixed with metalaxyl-mn-zn to be handled the tobacco after transplanting to improve to tobacco root rot
Preventive effect.
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| WO2004078790A1 (en) * | 2003-03-06 | 2004-09-16 | The Procter & Gamble Company | Chitosan powder |
| CN102986391A (en) * | 2011-09-08 | 2013-03-27 | 郑州大学 | Method for improving content and aromas of tobacco secondary metabolite products |
| CN105408421A (en) * | 2013-07-05 | 2016-03-16 | 博科科技有限公司 | Chitin nanowhisker composites and methods |
-
2016
- 2016-04-12 CN CN201610222996.XA patent/CN105746520B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004078790A1 (en) * | 2003-03-06 | 2004-09-16 | The Procter & Gamble Company | Chitosan powder |
| CN102986391A (en) * | 2011-09-08 | 2013-03-27 | 郑州大学 | Method for improving content and aromas of tobacco secondary metabolite products |
| CN105408421A (en) * | 2013-07-05 | 2016-03-16 | 博科科技有限公司 | Chitin nanowhisker composites and methods |
Non-Patent Citations (2)
| Title |
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| Nanoparticulate material delivery to plants;Remya Nair等;《Plant Science》;20101231;第179卷;第154-163页 * |
| 甲壳素及壳聚糖在农业领域方面的应用;蒋小姝等;《中国农学通报》;20130225;第29卷(第6期);第170-174页 * |
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| CN105746520A (en) | 2016-07-13 |
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