CN105746520A - Application of nano-chitin in improvement of yield and quality of tobaccos - Google Patents
Application of nano-chitin in improvement of yield and quality of tobaccos Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
- A01N25/04—Dispersions, emulsions, suspoemulsions, suspension concentrates or gels
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
- A01N37/46—N-acyl derivatives
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/28—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N<
- A01N47/34—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N< containing the groups, e.g. biuret; Thio analogues thereof; Urea-aldehyde condensation products
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/16—Heavy metals; Compounds thereof
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Abstract
The invention prepares a novel nano-biological material, namely nano-chitin by virtue of acidolysis deamidization. A nano-chitin water suspending agent is a nano-chitin whisker particle with a polycation property, the average size of whiskers of the particle is 100nm-150nm, the width is 15nm-3nm, and the comprehensive surface load capacity is 33mmol/kg. By processing tobacco seeds by virtue of the nano-chitin water suspending agent, the germination potential of the seeds can be improved; by processing tobacco seedlings in a seedbed stage, the growth vigor of the seedlings can be improved; by carrying out root-irrigation on transplanted tobacco seedlings, the plant height, the stem thickness, the number of leaves and the maximal leaf area of the tobaccos can be increased; by mixing the nano-chitin water suspending agent with thiophanate methyl for use, the synergistic interaction effect to the botanical characters of the tobaccos is achieved, and the application amount of chemical pesticides is reduced; and by mixing the nano-chitin water suspending agent with metalaxyl.Mn-Zn for processing transplanted tobaccos, the prevention effect to the root rot of the tobaccos can be improved, and the consumption of chemical bactericides can be reduced.
Description
Technical field
The invention belongs to technical field of tobacco, be specifically related to the application on tobacco leaf production of a kind of new bio nano material nanometer chitin, the especially nanometer chitin application in terms of improving tobacco production and quality.
Background technology
Bio-Nano-Materials is acknowledged as 21 century the most promising scientific research field, is forward position and the hot-topic subject in International Biotechnology field, and nanotechnology is causing a new industrial technology revolution.Nanosecond science and technology interpenetrate with agrotechnical, have also expedited the emergence of the new branch of science such as agriculture nanosecond science and technology, provide new theory, method and technological means for agricultural sciences, and the development of the future of agriculture will produce extensively far-reaching influence.Nano material has a big effect of special four: skin effect, bulk effect, quantum effect and macro quanta tunnel effect.These special effects make nano material have the unusual or unusual Wuli-Shili-Renli system approach not available for traditional material.
Chitin (chitin, structural formula is as shown in bottom left) it is the renewable polymer being widely present in nature, the main shell that source is the Crustaceans such as shrimp, Eriocheir sinensis, insecticide and molluscan organ (cartilage of such as sepiellae seu sepiae), and the cell wall etc. of Mycophyta.Its reserves natural polymer on earth accounts for second, estimates that the synthetic quantity of annual nature biotechnology is up to 1 × 1011Ton, is only second to cellulose.Under natural conditions, chitin is water insoluble, is also insoluble in acid or organic solvent, is therefore difficult to be used effectively.Chitosan (chitosan,
Structural formula is as shown in bottom right) it is chitinous derivant, under specific condition, chitin is degraded and de-acyl reaction obtains.Chitosan is soluble in weak acid.In weak acid, become positively charged polymer after the protonated amino on chitosan, thus there is biological activity, be widely used in the field such as medicine, plant pest management.
The same with cellulose, natural chitin is also to exist with filamentous form, is combined by N-acetyl glucosamine molecule in close by intermolecular and intramolecular hydrogen bond, is formed and have crystal and the chitin fiber of noncrystal two kinds of structures.Chitinous crystal structure has three kinds, i.e. α, β and γ type.The chitin of separate sources, its chemical constitution is different with character.Crusta Penaeus seu Panulirus shell is mainly α-type, and the gladius of squid is mainly β-type, and the chitin of fungal cell wall is mainly γ-type (degree of crystallinity is relatively low).α-type, β-type chitin is the abundantest, and degree of crystallinity is high, the most stable, is difficult to fusing, not readily dissolves, be not easy to be dissolved in common organic solvents simultaneously.α-type chitin resource is the abundantest, it is easy to industrialized production.
Pure chitinous processing technology is fairly simple, produce at present upper conventional method be by raw material (Crusta Penaeus seu Panulirus or Carapax Eriocheir sinensis) through deproteinization, desalination, the depigmentation, bleach, dry grinding and obtain chitin.Conventionally produced chitosan is chemistry de-acyl reaction or Hydrolyze method, and its principle is to destroy chitinous amorphous area by hydrolysis, makes molecular scission, and part N-acetyl glucosamine deacylated tRNA in strand improves hydrophilic.But chemistry deacylated tRNA or Hydrolyze method reaction condition require higher, should not control, and the response time is longer, power consumption, environmental pollution.Utilizing enzymatic isolation method can solve these problems, including using chitinase, chitosanase and lysozyme are hydrolyzed.But this fermentoid is expensive and is difficult to obtain, and causes product cost too high.
The method that hydrochloric acid hydrolysis is conventional prepares nanometer chitin.It is 150-800nm length that α-type chitin can produce size through acid hydrolysis, the chitin nano-particle of wide 5-70nm.The present invention is to use 3M HCl, in the ratio of 1:300 at 85-90oHydrolyze 2 hours under the conditions of C.Its product is 100-150nm length through dialysis, supersound process acquisition granularity, and chitin nano whisker wide for width 15-30nm, the consolidated statement density of surface charge is 33mmol/kg.
Due to chitinous natural character and unique molecular structure, increasingly paid attention to by scientific circles.Numerous studies show, chitin is in addition to having the performances such as nontoxic, bio-compatible, biodegradability, chitinous derivant (chitosan) can be used as the coating of the prevention and control of plant diseases, pest control, seed pelleting, fruits and vegetables in agricultural production, plant growing, inducing plant resistance and the effect of raising via plant immunity can also be improved.Nanometer chitin, in addition to inheriting chitinous fundamental property, also has the character of the highest specific surface area, high-crystallinity and polycation, is considered the natural macromolecular material of excellent combination property.Utilizing the nanometer chitin of hydrolysis preparation, specific surface area is relatively big and carries a certain amount of polycation, and its nano-particle has more significant biological activity than general chitosan.Detecting through toxicity, 0.3% nanometer chitin aqueous suspension agent is all higher than 5000mg/kg.BW to male and female rats acute footpath mouth LD50,
Acute percutaneous LD50 is all higher than 2000mg/kg.BW,
The most nontoxic to other biological.There are some researches show nano titanium oxide, multilayer carbon nanotube, metal nanoparticle and the nanometer induction apparatus based on nano material etc., these materials are the most all having application, but have the application on Nicotiana tabacum L. of the nanometer chitin of good biological characteristic and using value the most not by systematic study.
Nicotiana tabacum L. is the industrial crops that China is important, and pest and disease damage is the key factor affecting China's tobacco production and quality.Improvement to these pest and disease damages, chemical prevention is to produce one of upper major measure used, but extensively, in a large number with life-time service chemical pesticide causes agricultural chemical residue in tobacco and increase, and the Drug resistance of disease pest strengthens and rampant once again, i.e. " three R " problem.Research shows, has nearly 70 kinds of chemical substances that may interfere with human endocrine at present, and its Pesticides just has more than 40 to plant.The event causing pesticide intoxication in recent years due to food such as the use residual veterinary antibiotics exceeded standard of agriculture is of common occurrence.Although Nicotiana tabacum L. can not directly be eaten, but a lot of country still divides foodstuff or quasi-foodstuff into Nicotiana tabacum L., and as sucking product, Nicotiana tabacum L. occurs the chemical reaction of large amount of complex in combustion, produces many new chemical substances, and the pesticide of residual also can be participated.Pesticide residues index has become the important content in various countries Nicotiana tabacum L. and tobacco product quality control.Therefore modern agriculture replaces in the urgent need to new formulation efficient, eco-friendly or reduces chemistry and the use of fertilizer.
Summary of the invention
Present invention aim to overcome that prior art defect, it is provided that the application in terms of improving tobacco production and quality of a kind of nanometer chitin.
For achieving the above object, the present invention adopts the following technical scheme that
The application in terms of improving tobacco production and quality of the nanometer chitin.
Concrete, tobacco seed can be processed with 0.001-0.02% nanometer chitin water slurry, to improve the germinating energy of tobacco seed.
Concrete, seedbed phase tobacco seedling can be processed with 0.001-0.01% nanometer chitin water slurry, to improve the growing way of tobacco seedling.
Concrete, with 0.001-0.005% nanometer chitin water slurry, the tobacco seedling after transplanting can be carried out pouring root, to improve that the plant height of Nicotiana tabacum L., stem be thick, the number of blade and leaf area.
Concrete, 0.001-0.005% nanometer chitin water slurry and 36% thiophanate-methyl can be used with the tobacco seedling processed after transplanting, to improve the Agronomic trait of Nicotiana tabacum L., as thick in plant height, stem, the number of blade and leaf area etc., can reduce the applied amount of chemical pesticide simultaneously.
Concrete, mixed with metalaxyl-mn-zn for the 0.002% nanometer chitin water slurry Nicotiana tabacum L. processed after transplanting, to improve the preventive effect to Tobacco Root maize ear rot, can be reduced the consumption of chemical bactericide simultaneously.
The present invention utilizes new bio nano material nanometer chitin to improve the yield and quality of Nicotiana tabacum L., and utilize its synergistic function with common fungicide to prevent and treat tobacco rhizome disease, thus solve Social Events such as using chemicals, pesticide, chemical fertilizer etc. to have resulted in the increase of pest and disease damage resistance in a large number, soil degradation, environmental pollution, Practice for Pesticide Residue in Agricultural Products exceed standard.
Compared to the prior art, beneficial effects of the present invention:
1) nanometer chitin of the present invention is a kind of environmentally friendly nanometer formulation-water slurry, it is not necessary to any organic or inorganic auxiliary agent；2) on Nicotiana tabacum L., the application of ultralow consumption can improve the Agronomic trait of crop, increasing yield and improving quality, can reduce again the impact on soil, water and environment；3) can improve protection effect mixed with common fungicide, reduces the consumption of antibacterial simultaneously, reduces pesticide residues, destruction to human and environment and pollution are greatly reduced；4) less demanding to the technology of user, and can save time, saving of labor, laborsaving, meet Rural Areas at Present social economy reality；5) there is offer nutrient, improvement soil and the several functions of controlling crop diseases and insect pests；6) main employing agricultural wastes produce nano material, can promote recycling of agricultural wastes resource.
Accompanying drawing explanation
Fig. 1 is the facilitation that seedbed phase tobacco seedling is grown by nanometer chitin water slurry；
Fig. 2 is the impact on tobacco plant character of the nanometer chitin water slurry；
Fig. 3 is nanometer chitin water slurry and the mixed synergistic function to tobacco plant character of 36% thiophanate-methyl；
Fig. 4 is nanometer chitin water slurry synergistic function to preventing and treating tobacco diseases mixed with metalaxyl-mn-zn；
Fig. 5 is the chitinous transmission electron microscope picture of nanometer of the present invention, it can be seen that chitin nano-particle is whisker structure in figure, granularity is at 100-150nm length, 15-30nm width.
Detailed description of the invention
Below in conjunction with embodiment, technical scheme is discussed in detail further, but protection scope of the present invention is not limited thereto.
In following embodiment, nanometer chitin used obtains through following method:
The present invention uses 3M HCl, by raw material chitin (Sigma-Aldrich,
C9213-1KG) and 3M hydrochloric acid w/v 1g:300mL by both mix, under the conditions of 85-90 DEG C hydrolyze 2 hours.After hydrolysis terminates, product is dialysed with under regenerated cellulose dialyzer (MWCF12-14KDa) room temperature in deionized water, stable with the pH in the free acid of release to lye.Product after dialysis is under the conditions of 0 DEG C, supersound process 3-10 minute, then under the conditions of 4 DEG C, (12000rpm) is centrifuged 15 minutes at a high speed, after removing precipitation, i.e. obtaining granularity is 100-150nm length, chitin nano whisker water slurry (see figure 5) wide for width 15-30nm, the consolidated statement density of surface charge of its nanoparticle is 33mmol/kg.Measuring through acute toxicology, nanometer chitin aqueous suspensions is all higher than 5000mg/Kg.BW, acute percutaneous LD50 more than 2000mg/Kg.BW to female and male rat acute footpath mouth LD50.Therefore can be as novel tobacco adjuvant.
The nanometer chitin water slurry that the above-mentioned nanometer chitin preparing gained is configured to mass concentration 0.002%, 0.004%, 0.006%, 0.008%, 0.01% is carried out following application test.
Following embodiment, as a example by tobacco bred Zhongyan-100, carries out correlation test.
Embodiment
1
Nanometer chitin processes tobacco seed and improves potentiality of seed
Tobacco seed cultivated by the nanometer chitin water slurry of indoor 0.002-0.01%, compares with clear water comparison, and nanometer chitin can significantly improve Tobacco Seed Germination gesture (P < 0.05) (table 1).Concrete grammar: indoor are chosen substantial, full seed and are placed in centrifuge tube, with standby after distillation washing several times.Process tobacco seed with nanometer chitin, observe the germinating dynamics of seed.Respectively isopyknic 0.002%, 0.004%, 0.006%, 0.008%, 0.01% (wt/v) nanometer chitin water slurry is joined in the culture dish being lined with two layers of filter paper.Test compares with deionized water.Each process 3 repetition, 100 tobacco seeds of each repetition.Culture dish is put into (temperature is 27 DEG C, and relative humidity is 60%, light application time 12h) in PQX type multi-segment Programmable growth cabinet, supplement the nanometer chitin suspension of corresponding equivalent every 24h.The investigation method that Tobacco Seed Germination gesture is affected by nanometer chitin: observe the germination of seed every 12h record, reaches seed length for germinateing with radicle.Germinating energy is defined as: germinate most same day and the seed amount of all germinations before.
Table 1: the nanometer chitin impact on Tobacco Seed Germination gesture
Note: in table, capitalization English letter represents the significance level of difference, P < 0.01；Small English alphabet represents the significance level of difference, P < 0.05.
Embodiment
2
Nanometer chitin improves the growing way of seedbed phase tobacco seedling
Indoor 0.001-0.01% nanometer chitin processes seedbed phase tobacco seedling, compares with clear water comparison, and nanometer chitin has facilitation (see figure 1) to the growth of seedbed phase seedling, and different processing methods is different to the growth effect of seedling.Concrete grammar: sprayed by whole strain and execute two ways with root, is processed, with 0.001%, 0.003%, 0.005%, 0.01% nanometer chitin, the tobacco seedling that seedbed phase growing state is consistent respectively, observes the growing way of seedling after 30 days.Spraying standard: till blade face drop is the most saturated, every strain 3-4ml: root executes standard: every cave 10ml.Each process 5 repetition.
Embodiment
3
Nanometer chitin is thick on Nicotiana tabacum L. plant height, stem, the number of blade and the impact of leaf area
Under the natural conditions of field, with the nanometer chitin water slurry of 0.001%, 0.005%, the tobacco seedling after transplanting is carried out pouring root (100mL/ strain) respectively, manage by normal tobacco growing and topdress.Result of the test is shown in Table 2 and Fig. 2.
Nicotiana tabacum L. after test processes exceeds more than 16% at plant height, the thick aspect of stem than blank, and leaf area ratio comparison exceeds more than 33.76%, and the nanometer chitin of 0.001% promotes the effect of the plant growing nanometer chitin than 0.005% more preferably (Fig. 2).Nicotiana tabacum L. after spss software analysis can be processed is thick at plant height, stem, in the number of blade and leaf area and comparison reaches significant difference (table 2).
Table 2: nanometer chitin affects result to tobacco plant character
Note: in table, capitalization English letter represents the significance level of difference, P < 0.01；Small English alphabet represents the significance level of difference, P < 0.05.
Embodiment
4
Nanometer chitin and
36%
The mixed synergistic function to tobacco plant character of thiophanate-methyl
Under field conditions (factors), tobacco seedling after transplanting by constant antibacterial (36% thiophanate-methyl 800 times), 0.001%, 0.005% nanometer chitin water slurry and nanometer chitin and constant, decrement 25%, the antibacterial mixed processing of decrement 50% respectively, field is by the management of normal tobacco growing and topdresses, and result of the test is shown in Table 3 and Fig. 3.
Finding through test: compared with blank, the Nicotiana tabacum L. of 0.001-0.005% nanometer chitin and antibacterial (constant, decrement 25%, decrement 50%) mixed processing is thick at plant height, stem, reached significant difference (P < 0.05) in terms of the number of blade and leaf area.
Table 3: the synergistic function result of nanometer chitin and antibacterial
Remarks: T-0-0: blank；T-0-1:36% thiophanate-methyl；T-1-0:0.001% nanometer chitin water slurry；T-1-1:0.001% nanometer chitin water slurry and 36% thiophanate-methyl constant are used with；T-1-2:0.001% nanometer chitin water slurry and 36% thiophanate-methyl decrement 25% are used with；T-1-3:0.001% nanometer chitin water slurry and 36% thiophanate-methyl decrement 50% are used with；T-5-0:0.005% nanometer chitin water slurry；T-5-1:0.005% nanometer chitin water slurry and 36% thiophanate-methyl constant are used with；T-5-2:0.005% nanometer chitin water slurry and 36% thiophanate-methyl decrement 25% are used with；T-5-3:0.005% nanometer chitin water slurry and 36% thiophanate-methyl decrement 50% are used with
Embodiment
5
Nanometer chitin synergistic function to preventing and treating tobacco diseases mixed with metalaxyl-mn-zn
Under field conditions (factors), respectively with 0.002% nanometer chitin water slurry, antibacterial routine administration amount (metalaxyl-mn-zn, 1.5 kilograms/hectare) and the nanometer chitin water slurry Nicotiana tabacum L. that process transplant 70 day after mixed with the metalaxyl-mn-zn of decrement 25%, decrement 50%, field is by the management of normal tobacco growing and topdresses.Result of the test is shown in Table 4 and Fig. 4.
Find through test: the nanometer chitin obvious synergy to first frost MnZn, metalaxyl-mn-zn routine administration amount prevention effect mixed with nanometer chitin is 62.57%, is single use 1.13 times of metalaxyl-mn-zn prevention effect；Metalaxyl-mn-zn decrement 25% prevention effect mixed with nanometer chitin is 56.45%, and there is not significant difference between alone first frost MnZn prevention effect；Being used alone nanometer chitin and fusarium tabacinum root rot is also had certain preventive and therapeutic effect, preventive effect is 24.26%；First frost MnZn decrement 50% prevention effect pole mixed with nanometer chitin is remarkably decreased, and preventive effect is 14.07%(table 4).
Table 4: nanometer chitin and the antibacterial synergistic function result to preventing and treating Tobacco Root maize ear rot
Remarks: CK:72% first frost MnZn routine administration amount；Process two: 72% first frost MnZn routine administration amounts+nanometer chitin；The 75%+ nanometer chitin of three: the 72% first frost MnZn routine administration amounts of process；The 50%+ nanometer chitin of four: the 72% first frost MnZn routine administration amounts of process；Process one: nanometer chitin；CK0: clear water compares.
In summary it can be seen: nanometer chitin biomaterial of the present invention can promote tobacco growing, controlling disease, the consumption of minimizing chemical pesticide, reduces agricultural chemicals and uses the pollution to environment.If by antibacterial for nano material growth promotion and improve that the feature of environment is cementing with nano material or peplos slow/controlled release antibacterial combines, develop multifunctional nano antibacterial, using of chemical pesticide and growth regulator can be reduced, reduce soil it is adsorbed, alleviate the pollution of soil and groundwater, improve soil physical chemistry structure, improve soil and the crop utilization rate to nutrient.
Claims (6)
1. nanometer chitin application in terms of improving tobacco production and quality.
2. nanometer chitin application in terms of improving tobacco production and quality as claimed in claim 1, it is characterised in that process tobacco seed to improve the germinating energy of tobacco seed with 0.001-0.02% nanometer chitin water slurry.
3. nanometer chitin application in terms of improving tobacco production and quality as claimed in claim 1, it is characterised in that process seedbed phase tobacco seedling to improve the growing way of tobacco seedling with 0.001-0.01% nanometer chitin water slurry.
4. nanometer chitin application in terms of improving tobacco production and quality as claimed in claim 1, it is characterized in that, with 0.001-0.005% nanometer chitin water slurry the tobacco seedling after transplanting carried out pouring root to improve that the plant height of Nicotiana tabacum L., stem be thick, the number of blade and leaf area.
5. nanometer chitin application in terms of improving tobacco production and quality as claimed in claim 1, it is characterized in that, 0.001-0.005% nanometer chitin water slurry and 36% thiophanate-methyl use with process the tobacco seedling after transplanting to improve that the plant height of Nicotiana tabacum L., stem be thick, the number of blade and leaf area.
6. nanometer chitin application in terms of improving tobacco production and quality as claimed in claim 1, it is characterised in that the 0.002% nanometer chitin water slurry Nicotiana tabacum L. that process transplant after mixed with metalaxyl-mn-zn is to improve the preventive effect to Tobacco Root maize ear rot.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111116263A (en) * | 2020-01-17 | 2020-05-08 | 河南农业大学 | Application of nano chitin in improving fertilizer utilization rate |
| CN115005216A (en) * | 2022-06-22 | 2022-09-06 | 河南农业大学 | Application of nano chitin in promoting production of melons, fruits and vegetables |
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| CN115005216B (en) * | 2022-06-22 | 2023-08-08 | 河南农业大学 | Application of nano chitin in promoting melon fruit and vegetable production |
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| CN105746520B (en) | 2017-10-31 |
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