CN105746335A - Liquid pollination agent for early-maturing red pears - Google Patents

Liquid pollination agent for early-maturing red pears Download PDF

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Publication number
CN105746335A
CN105746335A CN201610129517.XA CN201610129517A CN105746335A CN 105746335 A CN105746335 A CN 105746335A CN 201610129517 A CN201610129517 A CN 201610129517A CN 105746335 A CN105746335 A CN 105746335A
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China
Prior art keywords
parts
pollination
liquid pollination
pollination agent
vitamin
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CN201610129517.XA
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Chinese (zh)
Inventor
井良才
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QUANJIAO JINGFU SELENIUM-RICH ECOLOGICAL ANIMAL HUSBANDARY Co Ltd
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QUANJIAO JINGFU SELENIUM-RICH ECOLOGICAL ANIMAL HUSBANDARY Co Ltd
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Priority to CN201610129517.XA priority Critical patent/CN105746335A/en
Publication of CN105746335A publication Critical patent/CN105746335A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02Methods or apparatus for hybridisation; Artificial pollination ; Fertility

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

The invention discloses a liquid pollination agent for early-maturing red pears.The liquid pollination agent comprises, by weight, 500-700 parts of cane sugar, 3-7 parts of potassium dihydrogen phosphate, 2-5 parts of calcium nitrate, 2-3 parts of amino acid chelate, 1.2-2.4 parts of guar gum, 0.3-1 parts of ethylene diamine tetraacetic acid, 1-2 parts of pear pollen particles, 1-2 parts of boric acid, 1-2 parts of multi-vitamin and 2000-2400 parts of water.The components and the preparation method of the liquid pollination agent are improved greatly as compared with those of the prior art.Compared with other pollination agents and pollination methods, the liquid pollination agent has the advantages that the liquid pollination agent is quick in action, high in pollination rate, low in production and labor cost and good in popularization value, and influence of natural environment conditions on the liquid pollination agent is low.

Description

A kind of early crisp red pear liquid pollination agent
Technical field
The invention belongs to field of planting, be specifically related to a kind of early crisp red pear liquid pollination agent.
Background technology
Pears are self-pollination sterility fruit trees, must plant pollinizers aborning or artificial supplementary pollination could obtain corresponding yield and quality.Planting pollinizers mainly utilizes the pollination ability of insecticide to carry out spontaneous pollination, although save labor, but is easy to be subject to the impact of weather to cause Pollination Fertilization bad.Increasing theatre adopts artificial supplementary pollination in recent years.Although the mode selectivity that in artificial supplementary pollination method, artificial point is awarded is strong, accuracy rate is high, and pollen consumption is little, but operable time is short, and recruitment is many, and production cost is higher.Additionally, the method that human assistance solid dusts there is also pollen consumption greatly, relatively costly problem.Liquid pollination is a kind of novel pollination technique, has pollination speed fast, saves artificial feature, and can increase moisture and the nutrient of stigma, extends the stigma Pollination Fertilization time.But liquid pollination technology is still immature, however it remains nutritional solution suspending power poor, pollen dispersion is uneven, the urgent problem such as swelling death in nutritional solution.
Summary of the invention
In order to solve the problems referred to above, the invention provides a kind of early crisp red pear liquid pollination agent, composition and compound method to liquid pollination agent have improved, and solve the easy swelling death of pollen, disperse uneven, and the problem that conventional liq pollination agent cost is high, service efficiency is low.
The present invention is achieved through the following technical solutions:
A kind of early crisp red pear liquid pollination agent, is made up of the material of following weight portion:
500 ~ 700 parts of sucrose, 3 ~ 7 parts of potassium dihydrogen phosphates, 2 ~ 5 parts of calcium nitrates, 2 ~ 3 parts of amino-acid chelates, 1.2 ~ 2.4 parts of guar gum, 0.3 ~ 1 part of ethylenediaminetetraacetic acid, 1 ~ 2 part of pear flower pollen particles, 1 ~ 2 part of boric acid, 1 ~ 2 part of compound vitamin, 2000 ~ 2400 parts of water;
Its preparation method comprises the steps:
(1) choose the petal that outward appearance is good, a bud just ready to burst, put in vial standby after flower pesticide being taken out with the tweezers after sterilization;
(2) when the flower pesticide chosen being placed on 20 ~ 25 DEG C, and slowly rub, after the pollen in flower pesticide ejects, then with 120 order dusting covers, impurity is sifted out, retain pear flower pollen particles standby;
(3) when pear flower pollen particles being placed on 23 ~ 26 DEG C, keeping relative air humidity is 80 ~ 90%, stand after moisture absorption 2 ~ 3h until it, add the guar gum of its gross mass 1.2 times, and blast warm wind, controlled wind speed is 8 ~ 10m/s, after continuing 5 ~ 10min mix homogeneously, obtains arogel mixture standby;
(4) 500 ~ 700 portions of sucrose, 3 ~ 7 parts of potassium dihydrogen phosphates, 2 ~ 5 parts of calcium nitrates, 2 ~ 3 parts of amino-acid chelates, 1 ~ 2 part of boric acid, 1 ~ 2 part of compound vitamin are joined in 2000 ~ 2400 parts of water, it is to slowly warm up to 20 ~ 23 DEG C, period does not stop stirring, after mentioned component fully dissolves, again arogel mixture is passed in aqueous solution with the speed of 4.5 ~ 5.5m/s, finally water solution mixture is placed in ultrasonic Treatment instrument and processes 60 ~ 80s namely obtains liquid pollination agent.
Further, described amino-acid chelate is made up of zinc-amino acid chelate, calcium amino acid chelate, chelating amino acids molybdenum, iron-amino acid chelate.
Further, containing vitamin B, vitamin C, vitamin E in described compound vitamin.
Further, the temperature of the warm wind described in step (3) is 23 ~ 26 DEG C.
Further, the frequency of the ultrasonic Treatment described in step (4) is 30 ~ 33KHz.
There is advantages that
(1) by after pear flower pollen particles moisture absorption, itself and guar gum are stirred by the warm wind of recycling large wind, can make guar gum granule uniform adsorption on pear flower pollen particles, improve the scattered uniformity of pollen particles, then the arogel mixture of gained is passed in aqueous solution with the speed of 4.5 ~ 5.5m/s, the guar gum granule being adsorbed on pollen particles absorbs water rapidly, formed one by one each other scattered appearance have sticking bulk granule, improve pollen particles uniformity in liquid solution and suspension, pass into that speed is too high or too low all can reduce pollen particles uniformity in liquid solution and stability.
(2) interpolation of potassium dihydrogen phosphate, calcium nitrate, amino-acid chelate, compound vitamin and boric acid is not only pollen sprouting on gynoecium and provides required nutrient substance, and can penetrate in pollen cell when ultrasonic Treatment, improve the activity of pollen, ensure that pollination success rate, ethylenediaminetetraacetic acid can eliminate the heavy metal impact on enzyme after being absorbed by plant, further increase efficiency and the success rate of pollination, additionally, ultrasonic Treatment further increases pollen particles scattered uniformity in liquid flux.
(3) form that the pear flower pollen particles of fluid present invention pollination agent wraps one layer of sticky gum with outer layer is dispersed in liquid solution, overcome in common liq pollination agent problems such as easily mutually sticking between pollen particles, block shower nozzle, inequality of dusting, consumption are excessive, and pollen particles attachment dynamics on gynoecium can be strengthened, improve pollination rate, even if meeting with rainy weather after pollination, its effect is not significantly reduced.
(4) the liquid pollination agent of the present invention is compared with other pollination agent and pollinating method, has the advantages that instant effect, pollination rate height, production and labour cost are low, and the impact by natural environmental condition is less, has good promotional value.
Detailed description of the invention
Embodiment 1
A kind of early crisp red pear liquid pollination agent, is made up of the material of following weight portion:
600 parts of sucrose, 5 parts of potassium dihydrogen phosphates, 2 parts of calcium nitrates, 3 parts of amino-acid chelates, 1.2 parts of guar gum, 0.5 part of ethylenediaminetetraacetic acid, 1 part of pear flower pollen particles, 2 parts of boric acid, 1.2 parts of compound vitamines, 2200 parts of water;
Its preparation method comprises the steps:
(1) choose the petal that outward appearance is good, a bud just ready to burst, put in vial standby after flower pesticide being taken out with the tweezers after sterilization;
(2) when the flower pesticide chosen being placed on 20 ~ 25 DEG C, and slowly rub, after the pollen in flower pesticide ejects, then with 120 order dusting covers, impurity is sifted out, retain pear flower pollen particles standby;
(3) when pear flower pollen particles being placed on 23 ~ 26 DEG C, keeping relative air humidity is 80 ~ 90%, stands after moisture absorption 2 ~ 3h until it, adding the guar gum of its gross mass 1.2 times, and blast warm wind, controlled wind speed is 10m/s, after continuing 5 ~ 10min mix homogeneously, obtain arogel mixture standby;
(4) 600 portions of sucrose, 5 parts of potassium dihydrogen phosphates, 2 parts of calcium nitrates, 3 parts of amino-acid chelates, 2 parts of boric acid, 1.2 parts of compound vitamines are joined in 2200 parts of water, it is to slowly warm up to 20 ~ 23 DEG C, period does not stop stirring, after mentioned component fully dissolves, again arogel mixture is passed in aqueous solution with the speed of 5m/s, finally water solution mixture is placed in ultrasonic Treatment instrument and processes 60 ~ 80s namely obtains liquid pollination agent.
Further, described amino-acid chelate is made up of zinc-amino acid chelate, calcium amino acid chelate, chelating amino acids molybdenum, iron-amino acid chelate.
Further, containing vitamin B, vitamin C, vitamin E in described compound vitamin.
Further, the temperature of the warm wind described in step (3) is 23 ~ 26 DEG C.
Further, the frequency of the ultrasonic Treatment described in step (4) is 30 ~ 33KHz.
The wind-force value impact on final liquid properties when preparing arogel granule for checking and be injected into solution, is provided with following several groups of comparative examples:
Comparative example 1 Comparative example 2 Comparative example 3 Comparative example 4 Comparative example 5
Prepare the wind speed (m/s) of arogel mixture 10 10 10 6 12
Arogel mixture injects the wind speed (m/s) in aqueous solution 3.5 6.5 8 5 5
In the comparative example of above-mentioned 1 ~ 5 group, except wind speed is different from embodiment 1, all the other composition, compound methods etc. are all identical, and pear flower flower in full bloom is carried out pollination experiment by liquid pollination agent respectively that prepare, and following table is corresponding correction data:
Embodiment 1 Comparative example 1 Comparative example 2 Comparative example 3 Comparative example 4 Comparative example 5
Flower percentage of fertile fruit (%) 94.2 86.5 84.7 77.6 88.8 94.4
As can be seen from the above table, to liquid pollination agent final utilization influential effect maximum be the wind speed size that arogel mixture is injected in aqueous solution, when its speed is too low, guar gum dissolves completely, pollen particles comparatively disperses, cohesiveness on pistil stigma is bad, and when running into rainy weather, effect can be worse;When its speed is too high, easily agglomerating between arogel granule, skewness, reduces Pollination Effect.When preparing rubber powder mixture, wind speed is too low not easily makes its mix homogeneously, though the uniformity improving mixing of wind speed too high energy low degree, the extra cost increased is excessive and wayward.
Embodiment 2
A kind of early crisp red pear liquid pollination agent, is made up of the material of following weight portion:
650 parts of sucrose, 6 parts of potassium dihydrogen phosphates, 3 parts of calcium nitrates, 2.5 parts of amino-acid chelates, 2.4 parts of guar gum, 1 part of ethylenediaminetetraacetic acid, 2 parts of pear flower pollen particles, 2 parts of boric acid, 1 part of compound vitamin, 2400 parts of water;
Its preparation method comprises the steps:
(1) choose the petal that outward appearance is good, a bud just ready to burst, put in vial standby after flower pesticide being taken out with the tweezers after sterilization;
(2) when the flower pesticide chosen being placed on 20 ~ 25 DEG C, and slowly rub, after the pollen in flower pesticide ejects, then with 120 order dusting covers, impurity is sifted out, retain pear flower pollen particles standby;
(3) when pear flower pollen particles being placed on 23 ~ 26 DEG C, keeping relative air humidity is 80 ~ 90%, stands after moisture absorption 2 ~ 3h until it, adding the guar gum of its gross mass 1.2 times, and blast warm wind, controlled wind speed is 9m/s, after continuing 5 ~ 10min mix homogeneously, obtain arogel mixture standby;
(4) 650 portions of sucrose, 6 parts of potassium dihydrogen phosphates, 3 parts of calcium nitrates, 2.5 parts of amino-acid chelates, 2 parts of boric acid, 1 part of compound vitamin are joined in 2400 parts of water, it is to slowly warm up to 20 ~ 23 DEG C, period does not stop stirring, after mentioned component fully dissolves, again arogel mixture is passed in aqueous solution with the speed of 4.8m/s, finally water solution mixture is placed in ultrasonic Treatment instrument and processes 60 ~ 80s namely obtains liquid pollination agent.
Further, described amino-acid chelate is made up of zinc-amino acid chelate, calcium amino acid chelate, chelating amino acids molybdenum, iron-amino acid chelate.
Further, containing vitamin B, vitamin C, vitamin E in described compound vitamin.
Further, the temperature of the warm wind described in step (3) is 23 ~ 26 DEG C.
Further, the frequency of the ultrasonic Treatment described in step (4) is 30 ~ 33KHz.
Comparative example 6
This comparative example adopts the composition identical with embodiment 2 and proportioning, the method adopting routine in the preparation, namely directly by 650 portions of sucrose, 6 parts of potassium dihydrogen phosphates, 3 parts of calcium nitrates, 2.5 parts of amino-acid chelates, 2.4 parts of guar gum, 1 part of ethylenediaminetetraacetic acid, 2 parts of pear flower pollen particles, 2 parts of boric acid, 1 part of compound vitamin and 2400 parts of water together mix homogeneously.
Comparative example 7
Using current conventional use of sucrose, boric acid, calcium chloride, pear flower pollen particles is as solute, water as solvent, and makes liquid pollination agent in the way of common mixing.
At early crisp red pear, the flowers are in blossom after 2 days, carries out spraying pollination to pear flower with the solvent described in embodiment 2, comparative example 6 and comparative example 7 respectively, and following table is correction data:
Embodiment 2 Comparative example 6 Comparative example 7
Flower percentage of fertile fruit (%) 94.5 72.3 60.8

Claims (5)

1. an early crisp red pear liquid pollination agent, it is characterised in that be made up of the material of following weight portion:
500 ~ 700 parts of sucrose, 3 ~ 7 parts of potassium dihydrogen phosphates, 2 ~ 5 parts of calcium nitrates, 2 ~ 3 parts of amino-acid chelates, 1.2 ~ 2.4 parts of guar gum, 0.3 ~ 1 part of ethylenediaminetetraacetic acid, 1 ~ 2 part of pear flower pollen particles, 1 ~ 2 part of boric acid, 1 ~ 2 part of compound vitamin, 2000 ~ 2400 parts of water;
Its preparation method comprises the steps:
(1) choose the petal that outward appearance is good, a bud just ready to burst, put in vial standby after flower pesticide being taken out with the tweezers after sterilization;
(2) when the flower pesticide chosen being placed on 20 ~ 25 DEG C, and slowly rub, after the pollen in flower pesticide ejects, then with 120 order dusting covers, impurity is sifted out, retain pear flower pollen particles standby;
(3) when pear flower pollen particles being placed on 23 ~ 26 DEG C, keeping relative air humidity is 80 ~ 90%, stand after moisture absorption 2 ~ 3h until it, add the guar gum of its gross mass 1.2 times, and blast warm wind, controlled wind speed is 8 ~ 10m/s, after continuing 5 ~ 10min mix homogeneously, obtains arogel mixture standby;
(4) 500 ~ 700 portions of sucrose, 3 ~ 7 parts of potassium dihydrogen phosphates, 2 ~ 5 parts of calcium nitrates, 2 ~ 3 parts of amino-acid chelates, 1 ~ 2 part of boric acid, 1 ~ 2 part of compound vitamin are joined in 2000 ~ 2400 parts of water, it is to slowly warm up to 20 ~ 23 DEG C, period does not stop stirring, after mentioned component fully dissolves, again arogel mixture is passed in aqueous solution with the speed of 4.5 ~ 5.5m/s, finally water solution mixture is placed in ultrasonic Treatment instrument and processes 60 ~ 80s namely obtains liquid pollination agent.
2. a kind of early crisp red pear liquid pollination agent according to claim 1, it is characterised in that described amino-acid chelate is made up of zinc-amino acid chelate, calcium amino acid chelate, chelating amino acids molybdenum, iron-amino acid chelate.
3. a kind of early crisp red pear liquid pollination agent according to claim 1, it is characterised in that containing vitamin B, vitamin C, vitamin E in described compound vitamin.
4. a kind of early crisp red pear liquid pollination agent according to claim 1, it is characterised in that the temperature of the warm wind described in step (3) is 23 ~ 26 DEG C.
5. a kind of early crisp red pear liquid pollination agent according to claim 1, it is characterised in that the frequency of the ultrasonic Treatment described in step (4) is 30 ~ 33KHz.
CN201610129517.XA 2016-03-08 2016-03-08 Liquid pollination agent for early-maturing red pears Pending CN105746335A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115568417A (en) * 2022-10-24 2023-01-06 广西南亚热带农业科学研究所 Macadamia nut liquid spray pollination method
EP4037473A4 (en) * 2019-10-01 2023-05-24 Monsanto Technology LLC Cross pollination through liquid-mediated delivery of pollen to enclosed stigmas of flowers from recipient plants

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102318549A (en) * 2011-08-16 2012-01-18 南京农业大学 Method with effects of saving cost and improving efficiency for liquid pollination of pear tree
CN102599046A (en) * 2012-03-16 2012-07-25 江苏省农业科学院 Pear pollination solution, and preparation method and application thereof
CN103642748A (en) * 2013-10-27 2014-03-19 大连东芳果菜专业合作社 Liquid pollen for accurate pollination
CN104137771A (en) * 2014-08-01 2014-11-12 岑建卫 Pollen filling agent for plant pollination and preparing technology and using method of pollen filling agent
CN104480184A (en) * 2014-12-16 2015-04-01 甘肃农业大学 Method for determining viability of pollen by carrying out in-vitro germination culture on brassica rapa pollen
CN104803740A (en) * 2015-03-12 2015-07-29 安徽农业大学 Pollen nutrient solution and application thereof to pear pollination

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Publication number Priority date Publication date Assignee Title
CN102318549A (en) * 2011-08-16 2012-01-18 南京农业大学 Method with effects of saving cost and improving efficiency for liquid pollination of pear tree
CN102599046A (en) * 2012-03-16 2012-07-25 江苏省农业科学院 Pear pollination solution, and preparation method and application thereof
CN103642748A (en) * 2013-10-27 2014-03-19 大连东芳果菜专业合作社 Liquid pollen for accurate pollination
CN104137771A (en) * 2014-08-01 2014-11-12 岑建卫 Pollen filling agent for plant pollination and preparing technology and using method of pollen filling agent
CN104480184A (en) * 2014-12-16 2015-04-01 甘肃农业大学 Method for determining viability of pollen by carrying out in-vitro germination culture on brassica rapa pollen
CN104803740A (en) * 2015-03-12 2015-07-29 安徽农业大学 Pollen nutrient solution and application thereof to pear pollination

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Title
徐汉卿: "《植物学》", 31 May 1996, 中国农业出版社 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4037473A4 (en) * 2019-10-01 2023-05-24 Monsanto Technology LLC Cross pollination through liquid-mediated delivery of pollen to enclosed stigmas of flowers from recipient plants
CN115568417A (en) * 2022-10-24 2023-01-06 广西南亚热带农业科学研究所 Macadamia nut liquid spray pollination method
CN115568417B (en) * 2022-10-24 2023-09-05 广西南亚热带农业科学研究所 Liquid spray pollination method for macadimia nuts

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Application publication date: 20160713