CN103980049B - Bi-enzyme polypeptide urea and preparation method thereof - Google Patents

Bi-enzyme polypeptide urea and preparation method thereof Download PDF

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Publication number
CN103980049B
CN103980049B CN201410222050.4A CN201410222050A CN103980049B CN 103980049 B CN103980049 B CN 103980049B CN 201410222050 A CN201410222050 A CN 201410222050A CN 103980049 B CN103980049 B CN 103980049B
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carbamide
enzyme
preparation
polypeptide
pelletize
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CN103980049A (en
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张�杰
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Ningming Of Guangxi Pepsi Health Biotechnology Co Ltd
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Ningming Of Guangxi Pepsi Health Biotechnology Co Ltd
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Abstract

The invention relates to the technical field of production of agricultural product fertilizer, and particularly relates to a bi-enzyme polypeptide urea and a preparation method thereof. The preparation method comprises the main steps of (1) preparing fungus liquid; (2) processing raw materials; (3) prilling; and (4) drying: drying mixed particles in a 50-60DEG C drying system for 10-15 minutes. The polypeptide urea prepared by the method has good solubility and more nutrients, can be absorbed by crops favorably, has low fertilizer application rate, and improves the yield of the agricultural products.

Description

A kind of pair of enzyme polypeptide carbamide and preparation method thereof
Technical field
The present invention relates to agricultural product technical field of fertilizer production, more particularly, to a kind of pair of enzyme polypeptide carbamide and its preparation side Method.
Background technology
Carbamide is a kind of high concentration nitrogenous fertilizer, belongs to neutral quick-acting fertilizer it can also be used to produce multiple compound fertilizers.Because having Nitrogen content is high, is in granular form fertilising conveniently, the advantages of not remaining any harmful substance in soil in measures for increasing agricultural output, quilt Using the most extensive.Peptide urea is in carbamide forming process, adds metalloproteases, concentrate through vaporizer in urine Pelletize forms.With the addition of metalloproteases is to greatly promote growth and development of plants.Through test, apply peptide urea, plant 5-15 days maturations typically can be shifted to an earlier date, and chemical fertilizer utilization ratio and crops quality etc. can be improved.But weak point sprays polypeptide Carbamide, the element absorption such as nitrogen that crops discharge to it, phosphorus, potassium is not comprehensive, and molten speed is slow, causes urea desorption column low, fertile Effect runs off seriously, causes crops prevalence high, promotes to develop the situation but do not increased production.
Content of the invention
In order to solve the existing issue existing for background technology, one aspect of the present invention provides a kind of pair of enzyme polypeptide urine Element is it is characterised in that be prepared from by the raw material of following components by weight percent:
Carbamide 95-98 part, metalloproteases 3-5 part, oxidoreductase 3-5 part, transferring enzyme 3-5 part, cellulase 3-5 Part, hydrolytic enzyme 3-5 part, urase 3-5 part and polypeptide 3-5 part.
Preferably, described double enzyme polypeptide carbamide are it is characterised in that be prepared from by the raw material of following components by weight percent: Carbamide 95-96 part, metalloproteases 4-5 part, oxidoreductase 3-4 part, transferring enzyme 3-4 part, cellulase 4-5 part, hydrolytic enzyme 3-4 part, urase 4-5 part and polypeptide 3-4 part.
On the other hand, there is provided the preparation method of this kind of pair of enzyme polypeptide carbamide it is characterised in that using above-mentioned raw material, Comprise the following steps:
1) preparation of bacterium feed liquid: take the ratio mixing as female strain and clear water with mass percent for 1: 200 for the yeast It is placed in sealing and fermenting 3-4 hour at a temperature of 35-40 DEG C, prepare bacterium feed liquid;
2) process of raw material: be sent in blender after choosing described raw material and its components by weight percent and bacterium feed liquid mix homogeneously It is stirred, the mixing speed of blender is arranged on 60-80 rev/min, obtains mixed material, with the addition of bacterium feed liquid, to product Provide source of organic matter, there is provided good humus, after administration can improved soil, improve the fertility of soil;
3) pelletize: mixed material is placed in rotary drum granulator and carries out pelletize, first preheated at a temperature of 30-35 DEG C Then naturally cool to room after pelletize 10-20 minute, then prilling temperature is set in 40-50 DEG C, pressure set is in 1.0- 1.5mpa, pelletize 40-60 minute, obtain hybrid particles, temperature control pelletize is preheated using segmentation, pelletize rate is high, urea efficiency is high, temperature Too low, pelletize is incomplete;Temperature is too high, can decomposite biuret, have impact on the fertilizer efficiency of product;
4) dry: hybrid particles are placed in and are dried 10~15 minutes inside 50-60 DEG C of drying system, then temperature is adjusted 10-15 minute is dried to 70-80 DEG C, finally again temperature is transferred to 100-120 DEG C and 20-30 minute is dried, obtain urea granules;
Preferably, described double enzyme polypeptide carbamide are it is characterised in that the enzyme in described raw material can be according to crop Difference needs to select two kinds therein or two or more.
Preferably, the preparation method of described double enzyme polypeptide carbamide is it is characterised in that be spaced in described granulation process 10-20 minute uniformly sprays clear water, sprays the 10-15% that clear water amount is mixed material mass percent.
Preferably, described double enzyme polypeptide carbamide preparation method it is characterised in that after pelletize hybrid particles particle diameter Reach the entrance next one baking step of 3-5mm, be not reaching in the return blender of described particle size range stir pelletize again.
Beneficial effects of the present invention, provide nitrogen source using raw material carbamide to agricultural cultivation, and polypeptide is to be related to organism The bioactive substance of interior various cell function.Peptide bond is main connected mode in protein molecule for the aminoacid, with the addition of Metalloproteases make the metal ion on peptide bond have very strong biological activity, promote urea molecule to enliven, metalloproteases Can directly be absorbed by plant, greatly promote growth and development of plants;In addition oxidoreductase, transferring enzyme, cellulase have been worked in coordination with Brightly embody the functions such as biological identification, catalysis, regulation, intensify chemical fertilizer, finally together with hydrolytic enzyme and urase, carbamide is urged Change and hydrolyze the nickeliferous enzyme generating carbonic acid and two molecules of ammonia so that the peptide urea dissolubility preparing is good, comprehensive nutrition, more Beneficial to the absorption of agricultural product plant, the fertile rate of mistake is low, improves the yield of agricultural product.
Specific embodiment
Embodiment
Process 1: take 950 grams of carbamide, 30 grams of metalloproteases, 30 grams of oxidoreductase, 30 grams of transferring enzyme, cellulase 30 Gram, 30 grams of hydrolytic enzyme, 30 grams of urase and 30 grams of polypeptide;
Process 2: 15 kilograms of common urea;
Process 3: take 900 grams of carbamide, 25 grams of metalloproteases, 25 grams of oxidoreductase, 25 grams of transferring enzyme and 25 grams of polypeptide;
Process 4: take 900 grams of carbamide, 30 grams of metalloproteases, 30 grams of cellulase, 30 grams of hydrolytic enzyme, 30 grams of urase and many 30 grams of peptide.
The preparation of bacterium feed liquid: take the ratio mixing as female strain and clear water with mass percent for 1: 200 for the yeast to put Sealing and fermenting 3 hours at a temperature of 35 DEG C, prepare bacterium feed liquid;
The process of raw material: be sent in blender after choosing described raw material and its components by weight percent and bacterium feed liquid mix homogeneously Row stir process, the mixing speed of blender is arranged on 60 revs/min, obtains mixed material, with the addition of bacterium feed liquid, provides to product Source of organic matter, there is provided good humus, after administration can improved soil, improve the fertility of soil;
Pelletize: mixed material is placed in rotary drum granulator and carries out pelletize, first carry out preheating pelletize at a temperature of 30 DEG C Afterwards and then naturally cool to room within 10 minutes, then prilling temperature is set in 40 DEG C, pressure set is in 1.0mpa, 40 points of pelletize Clock, obtains hybrid particles, preheats temperature control pelletize using segmentation, and pelletize rate is high, and urea efficiency is high, and temperature is too low, and pelletize is incomplete;Temperature Spend height, biuret can be decomposited, have impact on the fertilizer efficiency of product;
Dry: hybrid particles are placed in inside 50 DEG C of drying system and are dried 10 minutes, then temperature is transferred to 70 DEG C and does Dry 10 minutes, finally again temperature is transferred to 100 DEG C of dryings 20 minutes, obtains urea granules.
With above 4 different process, interval lab scale is carried out to 333 square metres of corn planting, the time of cultivating is 3-4 Month.
Species Bloom/d Plant height/cm Blade/ 100-grain weight/g Planting percent/% Yield/%
Process 1 35 63.5 7 36 98 1010.4
Process 2 45 76.4 5 28 86 800.1
Process 3 38 70.3 6 32 96 860.4
Process 4 40 68.4 6 34 95 910.3
Can analyze out by table, the Semen Maydiss after the double enzyme polypeptide carbamide fertilisings being provided using the present invention are urinated than common Element is advanced by 3-10 days with the Semen Maydiss that with the addition of after two to three sample enzyme fertilisings and blooms, and then every growth parameter(s) is all leading, volume increase Even more increase 26.3% for rate contrast common urea, illustrate that double enzyme polypeptide carbamide that the present invention provides have greatly facilitated agriculture The growth promoter of product, comprehensive nutrition, it is more favorable for the absorption of agricultural product plant, improve the yield of agricultural product.
Although embodiment of the present invention is disclosed as above, it is not restricted to listed in description and embodiment With, it can be applied to various suitable the field of the invention completely, for those skilled in the art, can be easily Realize other modification, therefore under the general concept being limited without departing substantially from claim and equivalency range, the present invention does not limit In specific details with shown here as the embodiment with description.

Claims (5)

1. the preparation method of a kind of pair of enzyme polypeptide carbamide is it is characterised in that described double enzyme polypeptide carbamide are by following components by weight percent Raw material be prepared from:
Carbamide 95-98 part, metalloproteases 3-5 part, oxidoreductase 3-5 part, transferring enzyme 3-5 part, cellulase 3-5 part, water Solution enzyme 3-5 part, urase 3-5 part and polypeptide 3-5 part, comprise the following steps:
1) preparation of bacterium feed liquid: take the ratio mixing as female strain and clear water with mass percent for 1: 200 for the yeast to be placed in Sealing and fermenting 3-4 hour at a temperature of 35-40 DEG C, prepares bacterium feed liquid;
2) process of raw material: be sent in blender after choosing described raw material and its components by weight percent and bacterium feed liquid mix homogeneously and carry out Stir process, the mixing speed of blender is arranged on 60-80 rev/min, obtains mixed material;
3) pelletize: mixed material is placed in rotary drum granulator and carries out pelletize, first carry out preheating pelletize at a temperature of 30-35 DEG C Then naturally cool to room after 10-20 minute, then prilling temperature is set in 40-50 DEG C, pressure set is in 1.0- 1.5mpa, pelletize 40-60 minute, obtain hybrid particles;
4) dry: hybrid particles are placed in and are dried 10~15 minutes inside 50-60 DEG C of drying system, then temperature is transferred to 70-80 DEG C is dried 10-15 minute, finally again temperature is transferred to 100-120 DEG C and 20-30 minute is dried, obtain urea granules.
2. the preparation method of according to claim 1 pair of enzyme polypeptide carbamide is it is characterised in that former by following components by weight percent Material is prepared from: carbamide 95-96 part, metalloproteases 4-5 part, oxidoreductase 3-4 part, transferring enzyme 3-4 part, cellulase 4- 5 parts, hydrolytic enzyme 3-4 part, urase 4-5 part and polypeptide 3-4 part.
3. the preparation method of according to claim 1 pair of enzyme polypeptide carbamide is it is characterised in that the enzyme in described raw material can To need to select two kinds therein or two or more according to the difference of crop.
4. the preparation method of according to claim 3 pair of enzyme polypeptide carbamide is it is characterised in that in the middle of described granulation process Uniformly spray clear water every 10-20 minute, spray the 10-15% that clear water amount is mixed material mass percent.
5. according to claim 3 pair of enzyme polypeptide carbamide preparation method it is characterised in that after pelletize hybrid particles grain Footpath reaches the entrance next one baking step of 3-5mm, is not reaching to stir in the return blender of described particle size range and again makes Grain.
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CN104892324A (en) * 2015-07-01 2015-09-09 德州鑫桥农化有限公司 Organic fertilizer capable of improving plant rhizosphere soil micro-environment
CN105819928A (en) * 2016-03-28 2016-08-03 山东禹城瑞利源科技有限公司 Application of compound chemical fertilizer in producing functional apples
CN109912348A (en) * 2019-04-10 2019-06-21 武鸣县红鹰肥业有限公司 Suitable for irrigating the organic fertilizer special of mandarin orange
CN109879698A (en) * 2019-04-10 2019-06-14 广西起凤橘洲生态农业有限公司 Organic fertilizer special suitable for citrus
CN114853526A (en) * 2021-01-20 2022-08-05 北京云图生态工程有限公司 Multi-enzyme active peptide combined fertilizer and preparation method thereof

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CN101155367B (en) * 2006-09-26 2011-07-13 中兴通讯股份有限公司 Identified number belonged network in multi-module mobile phone application and its method for selecting network when calling out
CN101875578A (en) * 2009-04-29 2010-11-03 崔明华 Ultra-efficient nitrogen fertilizer and manufacturing method thereof
CN102120711B (en) * 2010-12-10 2013-10-16 北京中农瑞利源高科技发展有限公司 Dual enzymes and double-enzyme serial fertilizer as well as application thereof in agriculture
CN103626566A (en) * 2012-08-21 2014-03-12 江苏新港金源化肥有限公司 Production method for ecological fertilizer containing medium and trace elements
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