CN105724047B - A kind of cultural method of high yield and high quality selenium-rich Cordceps militaris - Google Patents
A kind of cultural method of high yield and high quality selenium-rich Cordceps militaris Download PDFInfo
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Abstract
The invention discloses a kind of cultural method of high yield and high quality selenium-rich Cordceps militaris.Method is on the basis of Conventional solid cultivation, using sodium selenite as selenium source, soak at room temperature is carried out to selenium-enriching cultivation culture medium before sterilizing and handles 24~36h, and selenium-rich Cordceps militaris is cultivated using illumination volume increase cultural method, Cordceps militaris selenium-rich and Selenium endurance can be obviously improved, the excessively prosperous influence formed to annesl and former base of aerial hyphae is reduced, improves selenium-rich Cordceps militaris yield, selenium polysaccharide yield, cordycepin output, cordyceps sinensis acid yield and selenium-rich rate.Cultivation of selenium-rich Cordceps militaris is produced using this method, less investment is easy to operate, effect stability, and product has edible value, medical value and the economic value added of higher.
Description
Technical field
The present invention relates to fungus growing technique, is specifically exactly a kind of high yield and high quality selenium-rich cordyceps militaris cultivation method.
Technical background
Cordceps militaris Cordyceps militaris (Vuill.) Fr. also known as northern Chinese caterpillar Fungus, Cordceps militaris.People have been so far
Detect or isolate various bioactivators from Cordceps militaris, wherein except various amino acid, purine, inorganic salts and sterol etc. it
Outside, there are nucleosides, polysaccharide, alkaloid, ring-type contracting carboxylic peptide etc..Modern medicine shows that Cordceps militaris extract has multiple pharmacological effect,
Such as anti-hepatic fibrosis, suppress mankind's proliferation of glomerular mesangial cells, anti-angiogenesis, improves insulin resistance and insulin point
Secrete, suppress U937 leukemic cell growths, anti-inflammatory etc..
Illumination is to influence one of important environmental condition of Cordceps militaris growth and development, and research is found, light divides stroma of Cordyceps militaris
It is particularly significant to change, formed with shell of ascus maturation, and under non-illuminated conditions, Cordceps militaris can be only formed the bacterium of white on culture medium
Filament, at the same time, the color and luster of stroma is related with illumination power under optimum conditions.In addition, research confirms that intensity of illumination is to north
Cordyceps sinensis annesl, yield, cultivation period, stroma form, matrix utilization ratio have a significant impact, while blue light is that Cordceps militaris is normal
The requirement of annesl, and the synthesis of cordycepin can be promoted, green light is conducive to the extension of stroma, improves amylase activity, feux rouges
Be conducive to stroma thickening with yellow light.
Selenium is the essential trace elements of the human body, and selenium deficiency can cause a variety of diseases of human body to occur.Numerous studies confirm that selenium has
Many physiological functions such as anti-oxidant, anticancer, immunostimulation and suppression inhibition of HIV duplication.Research shows that inorganic selenium is that selenium element exists
The form being primarily present in nature, but Organic Selenium is but easy to animal alimentary canal absorption because having, compared with high toxicity threshold value and life
The advantages that thing utilization rate, obtained people and widely received and approve.In addition, research is found, organic selenium compounds are still formed
The important factor of body endogenous antioxidant defense system, so as to reduce interior free yl, active oxygen and active nitrogen free radical pair
The cellular component oxidative damage such as cell membrane, lipid, lipoprotein, DNA, and this and inflammation, cancer, angiocarpy and nerve
The generation of degenerative disease is closely related.Fungi has good enrichment function to mineral element, can absorb metallic element to son
In entity, and inorganic selenium is changed into Organic Selenium.Numerous studies show that, compared to common edible mushroom, selenium-enriched edible mushroom is respectively provided with more
High bioactivity.Important component of the selenium polysaccharide as Organic Selenium in selenium-enriched edible mushroom, has the double of both selenium and polysaccharide concurrently
Weight effect, research shows, compared to common polysaccharide, selenium polysaccharide has anti-oxidant, the antitumor and immune-enhancing activity of higher.Cause
This, developing selenium polysaccharide functional food using selenium-enriched edible mushroom as raw material or being processed into dietary supplements has become the certainty of future development
Trend.
Cordceps militaris has very strong selenium-rich and Selenium endurance, and medicine or health products have been developed using selenium-rich Cordceps militaris active material
Cause the extensive concern of industry, its potentiality to be exploited is big, has a extensive future.However, Cordceps militaris selenium-enriching cultivation production at present is still
Face many restrictions.Such as, inorganic selenium is because of relative low price, the main selenium cultivated all the time as selenium-enriched edible mushroom for a long time
Source, but inorganic selenium also has stronger toxicity to Cordceps militaris, adding too much will necessarily significantly influence yield.Therefore, not
While influencing Cordceps militaris yield and quality, organic selenium content and the selenium-rich rate of higher are obtained to lift selenium-rich Cordceps militaris quality still
So pendulum is in current practical problem.In addition, nitrogen source addition be Cordceps militaris growth and development, resistance and secondary metabolite it is special
It is the accumulation critical nutrients factor of cordycepin, especially in Cordceps militaris selenium-enriching cultivation, suitable increase nitrogen source is lifting selenium-rich worm
One of yield, quality and selenium-rich rate important means of grass, and this sees that light annesl goes out careless cultivation technique in conventional lucifuge bacterium germination
Aerial hyphae will necessarily be caused excessively prosperous and seriously affect selenium-rich Cordceps militaris yield.Though further more, there is the cultivation of selenium-rich Cordceps militaris so far
The research of training technology, and selenium-rich Cordceps militaris product is existing a small amount of occurs, but focused mostly in the past in using single yield and selenium-rich rate as
Index is studied, and research and molding technology in relation to its quality control do not occur but, its product quality is necessarily difficult to protect
Card.So in view of the above problems, under existence conditions, using a variety of method for increasing, selenium-rich cordyceps militaris cultivation bar is continued to optimize
Part, lifts its quality while selenium-rich cordyceps militaris cultivation yield is improved, is that current assistant officer need to solve the problems, such as.Therefore, it is of the invention
On the basis of original cultivation technique, using illumination yield-increasing technology, its cultivation condition is optimized, and comprehensive consideration production is real
Border, proposes the cultural method of a set of high yield and high quality Cordceps militaris, has certain realistic meaning and wide application prospect.
The content of the invention
It is an object of the invention to provide a kind of cultural method of the high-quality selenium-rich Cordceps militaris of high yield.
The technology used in the present invention is as follows：A kind of cultural method of high yield and high quality selenium-rich Cordceps militaris, method are as follows：Make
Basis cultivation nutrient solution, its formula contain：Potato 200g/L, glucose 20g/L, peptone 15g/L, potassium dihydrogen phosphate 2g/
L, magnesium sulfate 1g/L, Triammonium citrate 1g/L, vitamin B150mg/L；
Cultivated to basis in nutrient solution and add sodium selenite, sodium selenite ultimate density is 0.16g/L, and selenium-enriching cultivation is made
Nutrient solution；
Wheat is added in selenium-enriching cultivation nutrient solution, wheat is 3: 5g/mL with selenium-enriching cultivation nutrient solution solid-to-liquid ratio, room temperature leaching
When bubble 24~36 is small, autoclaving, is made solid wheat selenium-enriching cultivation culture medium, wherein containing sodium selenite 100mg/kg, it is cold
But it is followed by liquid spawn；
Training method is increased production using illumination：
Low-light culture is carried out in the bacterium germination stage, when light application time 24 is small/day, low-light intensity is 501x, and light quality is white
Color, is 16 DEG C in cultivation temperature, culture humidity is 30%~50%, cultivates 3~5 days, is covered with whole culture medium to mycelia；
The mycelia annesl stage carries out intense light irradiation culture, when light application time 24 is small/day, and intensity of illumination 3001x, light quality is white
Color, is 18 DEG C in cultivation temperature, culture humidity is 30%~50%, is cultivated 2~3 days, to media surface mycelia by faint yellow
It is changed into deep crocus；
Mycelia is alternately cultivated when inducing former base stage low-light and small intense light irradiation every 12, when light application time 24 is small/day,
Low-light intensity is 501x, and strong light intensity of illumination is 3001x, and light quality is white, is 18 DEG C in cultivation temperature, and culture humidity is
30%~50%, cultivate 10~12 days, formed completely to former base；
Mycelia stroma growth period is alternately cultivated using dark with intense light irradiation when 12 is small, when light application time 12 is small/
My god, and feux rouges is added when this period intense light irradiation culture, feux rouges intensity of illumination is 1001x, and white light intensity of illumination is 2001x, i.e.,
Total intensity of illumination is 3001x, is 22 DEG C in cultivation temperature, culture humidity is 85%~90%, is cultivated 30~35 days, to stroma top
End expansion and not regrowth；
Harvested, dried, up to high-quality selenium-rich Cordceps militaris dry product.
The present invention can obtain high yield and high quality selenium-rich Cordceps militaris, conventional selenium-enriching cultivation method is different from, before sterilization to selenium-rich
Culture medium for cultivating carries out soak at room temperature processing, can make sodium selenite well into wheat, relative reduction culture medium wheat top layer
Selenium concentration, so that under conditions of higher selenium additive amount, reduces cytotoxicity of the inorganic selenium to cordyceps sinensis, lifting selenium-rich Cordceps militaris production
Amount and quality；In bacterium germination stage, annesl stage and the former base stage is induced, using full exposure culture, can be greatly reduced because of nitrogen source
Increase and the influence for carrying out lucifuge culture and causing aerial hyphae excessively prosperous to cordyceps sinensis annesl and former base formation, promote substrate mycelium life
It is long, and then improve selenium-rich Cordceps militaris yield and selenium-rich rate；Selenium polysaccharide production is can further improve in stroma growth phase addition feux rouges
Amount and selenium-rich rate, so as to achieve the purpose that further increasing yield and improving quality.Cultivation of selenium-rich Cordceps militaris is produced using this method, there is input
Few, easy to operate, the advantages that effect stability, product also has edible value, medical value and the economic value added of higher.
Embodiment
Embodiment 1
It is as follows that bottle of the present invention plants concrete technology flow process：
Step 1：Peeling potatoes are cut into block, weigh potato 200g/L, is placed in appropriate tap water and boils 20 points
Clock, with three layers of filtered through gauze, sequentially adds glucose, peptone, dusty yeast, potassium dihydrogen phosphate, magnesium sulfate, triammonium citrate,
Vitamin B1, it is formulated as potato 200g/L, glucose 20g/L, peptone 15g/L, potassium dihydrogen phosphate 2g/L, magnesium sulfate 1g/
L, Triammonium citrate 1g/L, vitamin B150mg/L, is heated to dissolving, and basis cultivation nutrient solution is made；
Step 2：Sodium selenite is added in basis cultivation nutrient solution to step 1, sodium selenite ultimate density is
0.16g/L, is made selenium-enriching cultivation nutrient solution；
Step 3：500mL blake bottles are selected, every bottle adds wheat 30g, selenium-enriching cultivation nutrient solution 50mL, i.e. culture base-material
Liquor ratio is 3: 5, when soak at room temperature 24 is small after 121 DEG C of autoclavings 1.5 it is small when, solid wheat selenium-enriching cultivation culture medium is made, its
In contain sodium selenite 100mg/kg；
Step 4：In superclean bench, every bottle is uniformly accessed liquid spawn 5mL, and low-light training is carried out in the bacterium germination stage
Support, when light application time 24 is small/day, low-light intensity is 501x, and light quality is white, is 16 DEG C in cultivation temperature, and culture humidity is
30%, cultivate 5 days, be covered with whole culture medium to mycelia；
The mycelia annesl stage carries out intense light irradiation culture, when light application time 24 is small/day, and intensity of illumination 3001x, light quality is white
Color, is 18 DEG C in cultivation temperature, and culture humidity is 30%, is cultivated 3 days, is changed into deep orange to media surface mycelia from faint yellow
Color；
Mycelia is alternately cultivated when inducing former base stage low-light and small intense light irradiation every 12, when light application time 24 is small/day,
Low-light intensity is 501x, and strong light intensity of illumination is 3001x, and light quality is white, is 18 DEG C in cultivation temperature, and culture humidity is
30%, cultivate 12 days, formed completely to former base；
Mycelia stroma growth period is alternately cultivated using dark with intense light irradiation when 12 is small, when light application time 12 is small/
My god, and feux rouges is added when this period intense light irradiation culture, feux rouges intensity of illumination is 1001x, and white light intensity of illumination is 2001x, i.e.,
Always intensity of illumination is 3001x, is 22 DEG C in cultivation temperature, and culture humidity is 85%, is cultivated 35 days, to the expansion of stroma top and not
Regrowth；
Harvested, 45 DEG C of drying, up to high-quality selenium-rich Cordceps militaris dry product.
Embodiment 2
The potted plant concrete technology flow process of the present invention is as follows：
Step 1：Peeling potatoes are cut into block, weigh potato 200g/L, is placed in appropriate tap water and boils 20min,
With three layers of filtered through gauze, glucose, peptone, dusty yeast, potassium dihydrogen phosphate, magnesium sulfate, triammonium citrate, dimension life are sequentially added
Plain B1, it is formulated as potato 200g/L, glucose 20g/L, peptone 15g/L, potassium dihydrogen phosphate 2g/L, magnesium sulfate 1g/L, lemon
Lemon three ammonium 1g/L of acid, vitamin B150mg/L, is heated to dissolving, basic culture solution is made；
Step 2：Sodium selenite is added in basis cultivation nutrient solution to step 1, sodium selenite ultimate density is
0.16g/L, is made selenium-enriching cultivation nutrient solution；
Step 3：Culture basin is selected, wheat 500g, selenium-enriching cultivation nutrient solution 850mL, when soak at room temperature 36 is small are added per basin
When 121 DEG C of autoclavings 1 are small afterwards, solid wheat selenium-enriching cultivation culture medium is made, wherein containing sodium selenite 100mg/kg；
Step 5：In superclean bench, liquid spawn is uniformly accessed per basin, low-light culture is carried out in the bacterium germination stage,
When light application time 24 is small/day, low-light intensity is 501x, and light quality is white, is 16 DEG C in cultivation temperature, and culture humidity is
50%, cultivate 3 days, be covered with whole culture medium to mycelia；
The mycelia annesl stage carries out intense light irradiation culture, when light application time 24 is small/day, and intensity of illumination 3001x, light quality is white
Color, is 18 DEG C in cultivation temperature, and culture humidity is 50%, is cultivated 2 days, is changed into deep orange to media surface mycelia from faint yellow
Color；
Mycelia is alternately cultivated when inducing former base stage low-light and small intense light irradiation every 12, when light application time 24 is small/day,
Low-light intensity is 501x, and strong light intensity of illumination is 3001x, and light quality is white, is 18 DEG C in cultivation temperature, and culture humidity is
50%, cultivate 10 days, formed completely to former base；
Mycelia stroma growth period is alternately cultivated using dark with intense light irradiation when 12 is small, when light application time 12 is small/
My god, and feux rouges is added when this period intense light irradiation culture, feux rouges intensity of illumination is 1001x, and white light intensity of illumination is 2001x, i.e.,
Always intensity of illumination is 3001x, is 22 DEG C in cultivation temperature, and culture humidity is 90%, is cultivated 30 days, to the expansion of stroma top and not
Regrowth；
Harvested, 45 DEG C of drying, up to high-quality selenium-rich Cordceps militaris dry product.
Embodiment 3
Present invention culture high yield and high quality selenium-rich Cordceps militaris technique is drawn and good effect check test result is as follows：
1. the screening of culture medium soaking time before sterilizing
Influence of the different soaking times of table 1 to biological efficiency and selenium-rich rate
Note：CK represents non-selenium-rich and non-immersion treatment group；It is poor under 0.05 levels of P ＜ between same column tool same letter expression group
It is different not notable.
As shown in Table 1, Cordceps militaris selenium-rich and Selenium endurance can be obviously improved by carrying out immersion treatment to culture medium before sterilizing,
Under the conditions of selenium additive amount is 100mg/kg, when soaking time is 0h, its biological efficiency is only 76.23%, is slightly below compareed
Group (non-selenium-rich), illustrates on this condition, and inorganic selenium generates certain inhibitory action to Cordceps militaris growth；And during with immersion
Between its biological efficiency of increase and selenium-rich rate gradually step up, when soaking time is 24h, its biological efficiency and selenium-rich rate point
Not Gao Yu 0h treatment groups 17.17% and 19.97%, to 24h after tend to be steady, the results of analysis of variance shows, 24h treatment groups and
36h treatment groups difference is not notable (P ＜ 0.05)；This is probably since under conditions of total addition level is certain, sterilizing is preceding to culture
Base carries out immersion treatment, and with wheat water swelling, sodium selenite is stepped into wheat, relative reduction media surface selenium
Concentration, reduces the influence that Initial stage of culture inorganic selenium grows Cordceps militaris, and then improves selenium-rich Cordceps militaris yield and selenium-rich rate,
And non-immersion treatment group due to sterilizing when temperature quick increase, wheat top layer starch gelatinization process rapidly enters irreversible water suction
In the stage, have impact in the further wheat of sodium selenite, cause wheat top layer concentration of sodium selenite to have impact on Cordceps militaris higher than inside
Growth.Therefore, actual production is considered, culture medium soaking time is advisable with 24~36h before sterilizing.
2. most suitable selenium additive amount determines
Influence of the different selenium additive amounts of table 2 to biological efficiency and quality
Note：CK represents non-selenium-rich group；All treatment group culture mediums soak 24h；In P between same column tool same letter expression group
0.05 level differences of ＜ are not notable.
As shown in Table 2, selenium additive amount is remarkably improved Cordceps militaris biological efficiency, polysaccharide yield and cordyceps sinensis for 100mg/kg
Plain yield, 11.77%, 47.51% and 371.77% is improved compared to non-selenium-rich group respectively, and has higher selenium-rich rate, and
With selenium additive amount 50mg/kg conventional cultivation selenium-rich Cordceps militaris, the cordyceps sinensis acid yield of higher can be obtained.Therefore, this Process ba- sis is rich
Selenium culture medium for cultivating chooses selenium additive amount 100mg/kg, and carries out the optimization of next step selenium-enriching cultivation illumination condition.
3. the optimization of planting technique optimum illumination condition
(1) optimum illumination processing is definite
Table 3 cultivates optimum illumination processing screening test design
Note：Daily light application time is represented before "/"；" D " represents dark culturing；" S " represents intense light irradiation culture, and intensity of illumination is
3001x, light quality are white light；" W " represents low-light culture, and intensity of illumination 501x, light quality is white light；"-" represents alternate culture.
Influence of the different photo-irradiation treatments of table 4 to selenium-rich Cordceps militaris biological efficiency and upgrowth situation
Note：Difference is not notable under 0.05 levels of P ＜ between same column tool same letter expression group.
From table 3 and table 4, different photo-irradiation treatment group selenium-rich Cordceps militaris biological efficiencies are above conventional group, wherein locating
Manage 1 biological efficiency highest, be 95.65%, improve 8.19% than conventional group, place with outside treatment group 4, but between other each groups
Significant difference (P ＜ 0.05), meanwhile, treatment group 1, treatment group 2 and treatment group 4 are respectively provided with less aerial hyphae and higher son
Seat density, it can be seen that, the low-light processing of some strength is given in the bacterium germination stage can significantly reduce aerial hyphae amount, improve rich
Selenium Cordceps militaris yield.Further more, comparing treatment group 2,1 biological efficiency for the treatment of group improves 4.32%, significant difference (P ＜ 0.05)
And it is less with aerial hyphae amount, it follows that also there is necessity inducing former base stage addition low-light.In addition, variance
Analysis result shows, significant difference is not present in treatment group 1 and treatment group 4, biological efficiency and stroma density, and be respectively provided with compared with
Few aerial hyphae, and Integrated comparative treatment group 3 and treatment group 5, treatment group 5 are given birth to conventional organize knowable to each achievement data in stroma
Long stage addition low-light is not notable to selenium-rich Cordceps militaris yield effect, therefore considers production cost factor, in selenium-rich north worm
In grass cultivation stroma growth phase with illumination and light culture alternately for optimum illumination condition.In summary analyze, in selenium-rich
During cordyceps militaris cultivation, optimum illumination processing is：Bacterium germination stage (low-light/24h), annesl stage (intense light irradiation/24h), lure
Send out former base stage (low-light-intense light irradiation/24h), stroma growth phase (dark-intense light irradiation/12h).
(2) screening of low-light intensity
Influence of the different low-light intensity of table 5 to selenium-rich Cordceps militaris upgrowth situation
Note：Difference is not notable under 0.05 levels of P ＜ between same column tool same letter expression group.
As shown in Table 5, the influence significant difference that different bacterium germination stage intensities of illumination grow selenium-rich Cordceps militaris, in low-light
Cultivation stage, when intensity of illumination is 501x, its biological efficiency highest, is 94.26%, improves 6.72% than conventional group, and
Biological efficiency significant difference between other each treatment groups；With the increase of intensity of illumination, stroma color is gradually deepened, 701x processing
Group color is most deep；In each treatment group, 701x treatment group stromas density is maximum, is 0.091/cm2, is improved than conventional group
178.13%, but with 501x treatment groups without significant difference.In each treatment group, treatment group stroma length will be less than than conventional group,
701x treatment group stroma length is minimum, and 7.4% is have dropped than conventional group stroma length.Comprehensive consideration, when the illumination of bacterium germination stage is strong
Spend for 501x when, stroma length is slightly lower compared with other treatment groups, but its biological efficiency highest, and stroma density and 701x treatment groups
Compared to no significant difference, and selenium-rich rate is maximum at this time, is 25.58%, and therefore, bacterium germination stage intensity of illumination selects 501x.
(3) stroma growth phase addition light quality screening
Influence of the different addition light qualities of 6 stroma growth phase of table to biological efficiency and quality
Note：CK1Treatment group cultivation condition obtains full exposure selenium-enriching cultivation engineering test more than；Above-mentioned experiment is in total light intensity
Under the conditions of 3001x, that is, it is 1001x, the strong 2001x of white light to supplement light intensity；It is horizontal in P ＜ 0.05 between same column tool same letter expression group
Lower difference is not notable.
As shown in Table 6, different light medium is added in stroma growth phase to produce selenium-rich Cordceps militaris biological efficiency and cordycepic acid
Amount influence is smaller, significant difference is not present in the levels of P ＞ 0.05 between group, but different addition light qualities are to selenium-rich Cordceps militaris selenium polysaccharide
Yield and cordycepin output influence significantly, wherein, feux rouges and yellow light can be obviously improved selenium polysaccharide yield, carry respectively compared to the control group
High by 6.11% and 3.62%, the results of analysis of variance shows, feux rouges and yellow light treatment group, P ＞ 0.05, difference is not notable, the two
With blue light and control group significant difference (P ＜ 0.05).Blue light can be obviously improved cordycepin output, which reaches
0.39g·kg-1, 8.57%, 15.15% and 18.75% has been respectively increased compared to feux rouges, yellow light and control group, variance analysis knot
Fruit shows, significant difference (P ＜ 0.05) between blue light treatment group and other each groups, but feux rouges, yellow light and control group difference are not shown
Write (P ＞ 0.05).Furthermore feux rouges, which has selenium-rich rate, to be significantly affected, and up to 28.74%, improves 8.29% compared to the control group.By
This is as it can be seen that there is specificity in influence of the different addition light qualities to selenium-rich Cordceps militaris different activities substance production.In summary analyze,
It is the addition optimal light quality of illumination that can be obviously improved selenium-rich Cordceps militaris Cordyceps sinensis polysaccharide and selenium-rich rate in stroma growth phase addition feux rouges.
4. good effect is examined
(1) verification of technological parameter
The verification of 7 selenium-rich Cordceps militaris full exposure high-yield culturing processing parameter of table
Note：Difference is not notable under 0.05 levels of P ＜ between same column tool same letter expression group.
Empirical tests, using this method selenium-enriching cultivation Cordceps militaris, can be obviously improved its yield and quality, be planted compared to conventional selenium-rich
Training, its biological efficiency, selenium polysaccharide yield, cordycepin output, cordyceps sinensis acid yield and selenium-rich rate be respectively increased 17.65%,
20.59%th, 20.63%, 17.54% and 42.44%, good effect is notable, the reliable and stable pilot plant test for carrying out next step.
(2) pilot scale is cultivated
8 selenium-rich Cordceps militaris full exposure high-yield culturing production technology pilot scale experiment in cultivation of table
Table 8 is understood, in 3 cultivation bases, cultivation of selenium-rich Cordceps militaris, its biological efficiency, list are produced using the invention
Position quality compost dry weight yield and selenium-rich rate standard deviation are respectively 1.52%, 10.42gkg-1And 1.52%, it is relatively small,
It can be seen from the above that the technique good effect is stablized, reliably, production popularization can be carried out as selenium-rich Cordceps militaris high-yield culture technique and is answered
With.
Claims (1)
1. a kind of cultural method of high yield and high quality selenium-rich Cordceps militaris, method are as follows：Basis cultivation nutrient solution is made, its formula contains
Have：Potato 200g/L, glucose 20g/L, peptone 15g/L, potassium dihydrogen phosphate 2g/L, magnesium sulfate 1g/L, Triammonium citrate
1g/L, vitamin B150mg/L；
Sodium selenite is added it is characterized in that, being cultivated to basis in nutrient solution, sodium selenite ultimate density is 0.16g/L, is made
Selenium-enriching cultivation nutrient solution；
Wheat is added in selenium-enriching cultivation nutrient solution, wheat is 3: 5g/mL with selenium-enriching cultivation nutrient solution solid-to-liquid ratio, soak at room temperature 24
~36 it is small when, autoclaving, is made solid wheat selenium-enriching cultivation culture medium, wherein containing sodium selenite 100mg/kg, after cooling
Connect liquid spawn；
Training method is increased production using illumination：
Low-light culture is carried out in the bacterium germination stage, when light application time 24 is small/day, low-light intensity is 50lx, and light quality is white, is trained
It is 16 DEG C to support temperature, and culture humidity is 30%~50%, cultivates 3~5 days, is covered with whole culture medium to mycelia；
The mycelia annesl stage carries out intense light irradiation culture, when light application time 24 is small/day, and intensity of illumination 300lx, light quality is white,
Cultivation temperature is 18 DEG C, and culture humidity is 30%~50%, is cultivated 2~3 days, is changed into deep to media surface mycelia from faint yellow
Crocus；
Mycelia is alternately cultivated when inducing former base stage low-light and small intense light irradiation every 12, when light application time 24 is small/day, dim light
Be 50lx according to intensity, strong light intensity of illumination is 300lx, and light quality is white, and cultivation temperature is 18 DEG C, culture humidity for 30%~
50%, cultivate 10~12 days, formed completely to former base；
Mycelia stroma growth period is alternately cultivated using dark with intense light irradiation when 12 is small, when light application time 12 is small/day, and
Add feux rouges when this period intense light irradiation culture, feux rouges intensity of illumination is 100lx, and white light intensity of illumination is 200lx, i.e., total illumination
Intensity is 300lx, and cultivation temperature is 22 DEG C, and culture humidity is 85%~90%, is cultivated 30~35 days, to the expansion of stroma top and
Not regrowth；
Harvested, dried, up to high-quality selenium-rich Cordceps militaris dry product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610047530.0A CN105724047B (en) | 2016-01-15 | 2016-01-15 | A kind of cultural method of high yield and high quality selenium-rich Cordceps militaris |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201610047530.0A CN105724047B (en) | 2016-01-15 | 2016-01-15 | A kind of cultural method of high yield and high quality selenium-rich Cordceps militaris |
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| CN107333564B (en) * | 2017-07-19 | 2020-08-11 | 安康市农业科学研究所 | Production method of high-yield and high-selenium cordyceps militaris |
| CN107347446A (en) * | 2017-07-25 | 2017-11-17 | 南江宏信生物科技有限公司 | The cultural method of Cordceps militaris |
| CN109076881B (en) * | 2018-06-29 | 2021-06-01 | 江西天和食用菌开发有限公司 | Culture method and application of selenium-rich hericium erinaceus mycelium |
| CN109076882B (en) * | 2018-06-29 | 2021-09-03 | 江西天和食用菌开发有限公司 | Culture method and application of selenium-rich edible fungus mycelia |
| CN108998380A (en) * | 2018-08-09 | 2018-12-14 | 安康市农业科学研究所 | A kind of selenium-rich albefaction cordyceps culturing method |
| CN109964727B (en) * | 2019-04-22 | 2021-07-20 | 淮阴师范学院 | Method for improving selenium enrichment rate of cordyceps militaris by utilizing illumination |
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| CN101897275A (en) * | 2010-08-13 | 2010-12-01 | 义乌市丹溪药用生物开发研究所 | Selenium-rich cordyceps culturing method |
| CN102138436A (en) * | 2010-12-28 | 2011-08-03 | 湖北圣峰药业有限公司 | Method for culturing selenium-enriched Cordyceps militaris |
| CN102246659A (en) * | 2011-04-21 | 2011-11-23 | 金勇� | Method for producing selenium-enriched cordyceps |
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| KR20100104408A (en) * | 2009-03-17 | 2010-09-29 | 주식회사머쉬텍 | Cordyceps militaris mycellia cultured by using rice and poultry soup for health using the smae |
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| CN101897275A (en) * | 2010-08-13 | 2010-12-01 | 义乌市丹溪药用生物开发研究所 | Selenium-rich cordyceps culturing method |
| CN102138436A (en) * | 2010-12-28 | 2011-08-03 | 湖北圣峰药业有限公司 | Method for culturing selenium-enriched Cordyceps militaris |
| CN102246659A (en) * | 2011-04-21 | 2011-11-23 | 金勇� | Method for producing selenium-enriched cordyceps |
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| Title |
|---|
| 蛹虫草人工培养技术研究进展;周思静 等;《江苏农业科学》;20141231;第42卷(第7期);第13-17页 * |
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