CN105723213B - 高灵敏度电喷射接口 - Google Patents

高灵敏度电喷射接口 Download PDF

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Publication number
CN105723213B
CN105723213B CN201480059260.4A CN201480059260A CN105723213B CN 105723213 B CN105723213 B CN 105723213B CN 201480059260 A CN201480059260 A CN 201480059260A CN 105723213 B CN105723213 B CN 105723213B
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capillary
emitter
sheath
analyte
stream interface
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Chinese (zh)
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CN105723213A (zh
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诺曼·多维奇
孙良亮
朱贵杰
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University Notre Dame
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University Notre Dame
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • G01N30/72—Mass spectrometers
    • G01N30/7233—Mass spectrometers interfaced to liquid or supercritical fluid chromatograph
    • G01N30/724—Nebulising, aerosol formation or ionisation
    • G01N30/7266—Nebulising, aerosol formation or ionisation by electric field, e.g. electrospray
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/4473—Arrangements for investigating the separated zones, e.g. localising zones by electric means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44743—Introducing samples
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometers or separator tubes
    • H01J49/004—Combinations of spectrometers, tandem spectrometers, e.g. MS/MS, MSn
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometers or separator tubes
    • H01J49/02—Details
    • H01J49/04—Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locks; Arrangements for external adjustment of electron- or ion-optical components
    • H01J49/0404—Capillaries used for transferring samples or ions
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometers or separator tubes
    • H01J49/02—Details
    • H01J49/04—Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locks; Arrangements for external adjustment of electron- or ion-optical components
    • H01J49/0431—Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locks; Arrangements for external adjustment of electron- or ion-optical components for liquid samples
    • H01J49/0445—Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locks; Arrangements for external adjustment of electron- or ion-optical components for liquid samples with means for introducing as a spray, a jet or an aerosol
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometers or separator tubes
    • H01J49/02—Details
    • H01J49/10—Ion sources; Ion guns
    • H01J49/16—Ion sources; Ion guns using surface ionisation, e.g. field-, thermionic- or photo-emission
    • H01J49/165—Electrospray ionisation
    • H01J49/167—Capillaries and nozzles specially adapted therefor
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F04—POSITIVE - DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS FOR LIQUIDS OR ELASTIC FLUIDS
    • F04B—POSITIVE-DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS
    • F04B19/00—Machines or pumps having pertinent characteristics not provided for in, or of interest apart from, groups F04B1/00 - F04B17/00
    • F04B19/006—Micropumps

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Dispersion Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Plasma & Fusion (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
CN201480059260.4A 2013-08-29 2014-08-29 高灵敏度电喷射接口 Active CN105723213B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361871562P 2013-08-29 2013-08-29
US61/871,562 2013-08-29
PCT/US2014/053522 WO2015031820A1 (en) 2013-08-29 2014-08-29 High sensitivity electrospray interface

Publications (2)

Publication Number Publication Date
CN105723213A CN105723213A (zh) 2016-06-29
CN105723213B true CN105723213B (zh) 2019-09-13

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN201480059260.4A Active CN105723213B (zh) 2013-08-29 2014-08-29 高灵敏度电喷射接口

Country Status (6)

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US (2) US9465014B2 (ja)
EP (1) EP3042190B1 (ja)
JP (1) JP6601819B2 (ja)
CN (1) CN105723213B (ja)
HK (1) HK1226142A1 (ja)
WO (1) WO2015031820A1 (ja)

Families Citing this family (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105723213B (zh) * 2013-08-29 2019-09-13 圣母大学 高灵敏度电喷射接口
WO2016010748A1 (en) 2014-07-14 2016-01-21 Li-Cor, Inc. Analyte separator with electrohydrodynamic taylor cone jet blotter
JP6498497B2 (ja) * 2015-04-01 2019-04-10 学校法人慶應義塾 シースレスｃｅ−ｍｓ用スプレーデバイスの作成方法、シースレスｃｅ−ｍｓ用スプレーデバイス、及び、シースレスｃｅ−ｍｓ装置
JP6598253B2 (ja) * 2015-05-18 2019-10-30 株式会社Ｈｕｍａｎｉｘ １細胞あるいは超微小域分子の電場捕獲・遊離分離・分子検出法
RU2608362C2 (ru) * 2015-06-11 2017-01-18 Общество с ограниченной ответственностью "Альфа" (ООО "Альфа") Устройство стабильного электрораспыления при атмосферном давлении растворов веществ для источников ионов
EP3384274B1 (en) 2015-11-30 2021-11-10 Intabio, Inc. Methods for sample characterization
EP3398201A4 (en) 2015-12-31 2019-11-06 University of Notre Dame du Lac ESI-MS VIA ELECTROCINÉTICALLY PUMPED INTERFACE
WO2017136284A1 (en) * 2016-02-01 2017-08-10 Li-Cor, Inc. Capillary electrophoresis inkjet dispensing
CN106057630B (zh) * 2016-06-02 2017-10-27 中国检验检疫科学研究院 一种离子溅射镀膜毛细管喷雾电离装置
EP3497434B1 (en) 2016-08-08 2021-05-19 Li-Cor, Inc. Microchip electrophoresis inkjet dispensing
CA3031226A1 (en) 2016-08-08 2018-02-15 Li-Cor, Inc. Multi-sheath flow and on-chip terminating electrode for microfluidic direct-blotting
WO2018057885A1 (en) * 2016-09-23 2018-03-29 University Of Notre Dame Du Lac One-step capillary isoelectric focusing and mobilization of analytes
CN109791132A (zh) * 2016-09-26 2019-05-21 圣母大学 用于缓解毛细管区电泳-电喷雾装置中电流反转的方法和装置
US11073522B2 (en) * 2016-10-03 2021-07-27 Lincoln Memorial University Structural validation of very long chain dicarboxylic acids
CN106783511B (zh) * 2017-02-27 2018-07-13 中国计量科学研究院 基于极性反转电压策略的纳喷雾离子源及其操作方法
US11342173B2 (en) * 2017-07-07 2022-05-24 Dh Technologies Development Pte. Ltd. Electrospray interface device and associated methods
US20190019662A1 (en) * 2017-07-14 2019-01-17 Purdue Research Foundation Electrophoretic mass spectrometry probes and systems and uses thereof
WO2019148198A1 (en) 2018-01-29 2019-08-01 Intabio, Inc. Devices, methods and kits for sample characterization
CN108201654B (zh) * 2018-03-28 2023-10-13 南方医科大学南方医院 一种多用途喷洒导管
US10658168B2 (en) * 2018-05-03 2020-05-19 Perkinelmer Health Sciences Canada, Inc. Multiple gas flow ionizer
US11222779B2 (en) 2018-05-17 2022-01-11 GMJ Technologies, Inc. Apparatuses for optical and mass spectrometry detection
EP4439060A2 (en) 2018-05-31 2024-10-02 Intabio, Llc Software for microfluidic systems interfacing with mass spectrometry
US11117131B2 (en) 2018-08-22 2021-09-14 International Business Machines Corporation Electrokinetically separating, encapsulating and extracting analytes on a microfluidic device
US11640901B2 (en) 2018-09-05 2023-05-02 Protein Metrics, Llc Methods and apparatuses for deconvolution of mass spectrometry data
US11346844B2 (en) * 2019-04-26 2022-05-31 Protein Metrics Inc. Intact mass reconstruction from peptide level data and facilitated comparison with experimental intact observation
US20220120758A1 (en) * 2019-06-06 2022-04-21 Fudan University Method and device for protein sequence analysis
US11285484B2 (en) 2019-08-12 2022-03-29 Intabio, Llc Multichannel isoelectric focusing devices and high voltage power supplies
US11087964B2 (en) * 2019-11-21 2021-08-10 Thermo Finnigan Llc Method and apparatus for improved electrospray emitter lifetime
US11340200B2 (en) * 2020-03-08 2022-05-24 The Board Of Regents Of The University Of Oklahoma Electrospray assisted capillary device for processing ultra low-volume samples
CN111266140B (zh) * 2020-03-09 2021-07-16 厦门大学 无油分选-直接注入-icpms单细胞分析系统
CN111413172A (zh) * 2020-04-17 2020-07-14 山东省分析测试中心 一种原位酶解-直接质谱分析系统及分析方法
US11988652B2 (en) 2020-11-18 2024-05-21 Thermo Finnigan Llc Packed tip electrospray emitter
CN114496717A (zh) * 2022-01-18 2022-05-13 中国科学院成都生物研究所 电喷雾的激发装置及离子化方法
US20230243844A1 (en) * 2022-02-02 2023-08-03 Waters Technologies Corporation Nanospray reactive sprayer

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5115131A (en) * 1991-05-15 1992-05-19 The University Of North Carolina At Chapel Hill Microelectrospray method and apparatus
US5245186A (en) * 1991-11-18 1993-09-14 The Rockefeller University Electrospray ion source for mass spectrometry
CN102324375A (zh) * 2011-07-22 2012-01-18 中国科学院上海有机化学研究所 一种质谱纳喷雾电极及其制作方法和应用
WO2013075081A2 (en) * 2011-11-18 2013-05-23 Arizona Board Of Regents, A Body Corporate Of The State Of Arizona, Acting For And On Behalf Of Arizona State University System and method for providing a micron-scale continuous liquid jet

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4885076A (en) * 1987-04-06 1989-12-05 Battelle Memorial Institute Combined electrophoresis-electrospray interface and method
US5444208A (en) * 1993-03-29 1995-08-22 Fmc Corporation Multiple source plasma generation and injection device
US5423964A (en) * 1993-08-02 1995-06-13 Battelle Memorial Institute Combined electrophoresis-electrospray interface and method
DE4444229C2 (de) * 1994-03-10 1996-07-25 Bruker Franzen Analytik Gmbh Verfahren und Vorrichtungen zur Elektrosprüh-Ionisierung für speichernde Massenspektometer
US5868322A (en) * 1996-01-31 1999-02-09 Hewlett-Packard Company Apparatus for forming liquid droplets having a mechanically fixed inner microtube
US6690006B2 (en) * 2001-05-24 2004-02-10 New Objective, Inc. Method and apparatus for multiple electrospray sample introduction
GB0226160D0 (en) * 2002-11-08 2002-12-18 Diagnoswiss Sa Apparatus for dispensing a sample in electrospray mass spectrometers
JP2004317469A (ja) * 2003-04-10 2004-11-11 Ajinomoto Co Inc 質量分析用ナノ・スプレーイオン化用極細管
JP3991023B2 (ja) * 2003-10-31 2007-10-17 株式会社日立ハイテクノロジーズ 質量分析装置
WO2005101017A1 (en) * 2004-04-07 2005-10-27 Genentech, Inc. Mass spectrometry of antibody conjugates
JP3871689B2 (ja) * 2004-08-25 2007-01-24 ヒューマン・メタボローム・テクノロジーズ株式会社 電気泳動測定によるイオン性化合物の移動時間予測方法
US9885085B2 (en) * 2006-06-28 2018-02-06 University Of Utah Research Foundation Apparatus, compositions, and methods for assessment of chronic obstructive pulmonary disease progression among rapid and slow decline conditions
CA2717482C (en) 2008-03-07 2016-11-22 The University Of British Columbia Self-contained capillary electrophoresis system for interfacing with mass spectrometry
US9234880B2 (en) * 2011-06-03 2016-01-12 University Of Washington Through Its Center For Commercialization Sheath-flow electrospray interface
EP2844373A4 (en) 2012-05-03 2015-12-16 Medimmune Llc METHOD FOR ANALYZING SAMPLE COMPONENTS
CN105723213B (zh) * 2013-08-29 2019-09-13 圣母大学 高灵敏度电喷射接口

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5115131A (en) * 1991-05-15 1992-05-19 The University Of North Carolina At Chapel Hill Microelectrospray method and apparatus
US5245186A (en) * 1991-11-18 1993-09-14 The Rockefeller University Electrospray ion source for mass spectrometry
CN102324375A (zh) * 2011-07-22 2012-01-18 中国科学院上海有机化学研究所 一种质谱纳喷雾电极及其制作方法和应用
WO2013075081A2 (en) * 2011-11-18 2013-05-23 Arizona Board Of Regents, A Body Corporate Of The State Of Arizona, Acting For And On Behalf Of Arizona State University System and method for providing a micron-scale continuous liquid jet

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
A Low-Flow CE/Electrospray Ionization MS Interface for Capillary Zone Electrophoresis,Large-Volume Sample Stacking, and Micellar Electrokinetic Chromatography;Yet-Ran Chen 等;《ANALYTICAL CHEMISTRY》;20030201;第75卷(第3期);摘要，第504~506页，图1 *

Also Published As

Publication number Publication date
EP3042190C0 (en) 2024-04-03
US9465014B2 (en) 2016-10-11
US20150233877A1 (en) 2015-08-20
CN105723213A (zh) 2016-06-29
US20150311056A1 (en) 2015-10-29
WO2015031820A1 (en) 2015-03-05
US9362102B2 (en) 2016-06-07
JP2016534353A (ja) 2016-11-04
EP3042190B1 (en) 2024-04-03
EP3042190A4 (en) 2017-04-05
HK1226142A1 (zh) 2017-09-22
EP3042190A1 (en) 2016-07-13
JP6601819B2 (ja) 2019-11-06

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