CN105699473A - 胃癌唾液蛋白指纹图谱分子诊断模型建立方法 - Google Patents
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- G—PHYSICS
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- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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Abstract
Description
组别 | 例数 | 正确诊断(例) | 错误诊断(例) | 正确率(%) |
胃癌组 | 57 | 55 | 2 | 96 |
慢性胃炎组 | 28 | 24 | 4 | 86 |
组别 | 例数 | 正确诊断(例) | 错误诊断(例) | 正确率(%) |
胃癌组 | 57 | 51 | 6 | 89 |
慢性胃炎组 | 28 | 21 | 7 | 75 |
Claims (7)
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Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106950380A (zh) * | 2017-03-02 | 2017-07-14 | 先思达(南京)生物科技有限公司 | 一种胃癌监测试剂盒及其使用方法 |
CN110220965A (zh) * | 2019-07-17 | 2019-09-10 | 山东省分析测试中心 | 一种快速鉴别反复冻融肉类的方法 |
CN110880356A (zh) * | 2018-09-05 | 2020-03-13 | 南京格致基因生物科技有限公司 | 对卵巢癌进行筛查、诊断或风险分级的方法和装置 |
CN113466465A (zh) * | 2021-01-20 | 2021-10-01 | 浙江大学滨海产业技术研究院 | 一种基于蛋白质组学的唾液检测方法 |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104007169A (zh) * | 2014-05-29 | 2014-08-27 | 深圳市第二人民医院 | 一种胃癌的唾液蛋白诊断模型及其建立方法 |
CN104007166A (zh) * | 2014-05-29 | 2014-08-27 | 深圳市第二人民医院 | 一种慢性胃炎诊断的唾液蛋白诊断模型及其构建方法 |
CN104515797A (zh) * | 2014-05-29 | 2015-04-15 | 深圳市第二人民医院 | 乳腺癌早期诊断的唾液蛋白指纹图谱模型及其构建方法 |
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2016
- 2016-03-23 CN CN201610169314.3A patent/CN105699473B/zh active Active
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104007169A (zh) * | 2014-05-29 | 2014-08-27 | 深圳市第二人民医院 | 一种胃癌的唾液蛋白诊断模型及其建立方法 |
CN104007166A (zh) * | 2014-05-29 | 2014-08-27 | 深圳市第二人民医院 | 一种慢性胃炎诊断的唾液蛋白诊断模型及其构建方法 |
CN104515797A (zh) * | 2014-05-29 | 2015-04-15 | 深圳市第二人民医院 | 乳腺癌早期诊断的唾液蛋白指纹图谱模型及其构建方法 |
Non-Patent Citations (1)
Title |
---|
杨长青: "基于唾液蛋白质组学的2型糖尿病病证结合分子诊断模型研究", 《万方数据》 * |
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106950380A (zh) * | 2017-03-02 | 2017-07-14 | 先思达(南京)生物科技有限公司 | 一种胃癌监测试剂盒及其使用方法 |
CN106950380B (zh) * | 2017-03-02 | 2019-01-11 | 江苏先思达生物科技有限公司 | 一种胃癌监测试剂盒及其使用方法 |
CN110880356A (zh) * | 2018-09-05 | 2020-03-13 | 南京格致基因生物科技有限公司 | 对卵巢癌进行筛查、诊断或风险分级的方法和装置 |
CN110220965A (zh) * | 2019-07-17 | 2019-09-10 | 山东省分析测试中心 | 一种快速鉴别反复冻融肉类的方法 |
CN110220965B (zh) * | 2019-07-17 | 2020-05-22 | 山东省分析测试中心 | 一种快速鉴别反复冻融肉类的方法 |
CN113466465A (zh) * | 2021-01-20 | 2021-10-01 | 浙江大学滨海产业技术研究院 | 一种基于蛋白质组学的唾液检测方法 |
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Effective date of registration: 20240422 Address after: No. 3002 Sungang West Road, Huafu Street, Futian District, Shenzhen City, Guangdong Province, 518032 Patentee after: Shenzhen Second People's Hospital (Shenzhen Institute of translational medicine) Country or region after: China Address before: 518020 No. 1017 Dongmen North Road, Shenzhen, Guangdong, Luohu District Patentee before: SHENZHEN INSTITUTE OF GERIATRICS Country or region before: China Patentee before: Wu Zhengzhi |