CN105669273A - Culture medium for edible mushrooms and culture method thereof - Google Patents
Culture medium for edible mushrooms and culture method thereof Download PDFInfo
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- CN105669273A CN105669273A CN201610025998.XA CN201610025998A CN105669273A CN 105669273 A CN105669273 A CN 105669273A CN 201610025998 A CN201610025998 A CN 201610025998A CN 105669273 A CN105669273 A CN 105669273A
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- mussaendae pubescentis
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D3/00—Calcareous fertilisers
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
Abstract
The invention relates to a culture medium for edible mushrooms. The culture medium comprises the following ingredients (by weight): 15-70 parts of sawdust, 4-23 parts of trimmed branch and leaf powder of white tea, 1-7 parts of white tea powder, 18-25 parts of wheat bran, 0-2 parts of gypsum powder and 1-2 parts of cane sugar. The culture method of the culture medium for edible mushrooms comprises the following steps: 1, weighing the above ingredients in proportion and uniformly stirring, adding water and uniformly mixing; 2, putting the mixture into a bag and pressing, and carrying out sterilization treatment; 3, cooling, punching the sterilization bag to form inoculation holes, inoculating culture spawn for edible mushrooms into the inoculation holes, and sealing the inoculation holes with an aseptic adhesive tape; and 4, culturing in an environment of 22-26 DEG C, cutting off the bag after culture, discharging mycelia and the culture medium out of the bag, and culturing together in a greenhouse. Edible mushrooms obtained by the use of the culture medium have good mouthfeel, have thicky texture, have fragrance and have high edibleness.
Description
Technical field
The present invention relates to the cultivation matrix of edible fungi and cultivation method field, particularly to cultivation matrix and the cultural method of a kind of edible fungi.
Background technology
Edible fungi refers to mushroom fungus that can be edible, is commonly referred to as mushroom. the known edible fungi of China has kind more than 350, wherein belongs to Basidiomycotina more, common are: Lentinus Edodes, Volvariella volvacea (Bull.Ex Franch.) Singer．, mushroom, Auricularia, Tremella, hedgehog hydnum, Caulis Bambusae In Taeniam, Trichotoma matsutake, Tricholoma mongolicum Imai, Russula, Ganoderma, Cordyceps, Truffe, lark and bolete etc., minority belongs to Ascomycotina, Qi Zhongyou: Morchella esculenta (L.) Pers, saddle fungus, truffles etc. investment is little, the cycle is short, the project of having got rich of instant effect is able to fast development in China as one for edible fungus culturing, and once once supply falls short of demand for edible fungus. mushroom industry is a short, adaptable and fast rural economic development project integrating economic benefit, ecological benefits and social benefit, edible fungi is again that a class is organic, nutrition, health care pollution-free food. development mushroom industry meets people and consumes the needs increased with agricultural sustainable development, it it is the effective way quickly got rich of peasant, along with edible fungi market demand is increasing, cultivated area continues to increase greatly, conventional plantation raw material resources are fewer and feweri, find the existing raw material resources that new raw material replaces or supplements, become extremely urgent thing, in existing fungus growing technique, mushroom body absorbance is low simultaneously, cause that cost is high, and mouthfeel is not good, such as publication No. is: CN104557258A(2015-04-29) disclosed in a kind of method utilizing potato residues to prepare edible fungus culturing substrate, although solving the cultivation raw material sources of locality, but the edible fungi mouthfeel turned out haves much room for improvement.
Summary of the invention
Present invention aim to address the problems referred to above, it is provided that the cultivation matrix of a kind of edible fungi and cultural method, using the teaching of the invention it is possible to provide a kind of plantation raw material resources being different from routine, and the edible fungi quality that cultivation obtains is abundant, mouthfeel is fresh and tender.
The above-mentioned technical purpose of the present invention has the technical scheme that
A kind of cultivation matrix of edible fungi, it includes following component and weight portion:
Wood sawdust 15-70 weight portion
Ramulus et Folium Mussaendae Pubescentis trimming leaf end 4-23 weight portion
Ramulus et Folium Mussaendae Pubescentis powder 1-7 weight portion
Testa Tritici 18-25 weight portion
Gypsum Fibrosum powder 0-2 weight portion
Sucrose 1-2 weight portion.
Branch and leaf end and the Ramulus et Folium Mussaendae Pubescentis powder that the Ramulus et Folium Mussaendae Pubescentis of this programme is pruned is branch and leaf end and the Anji Ramulus et Folium Mussaendae Pubescentis powder of Anji Ramulus et Folium Mussaendae Pubescentis pruning, the branch and leaf end pruned by Ramulus et Folium Mussaendae Pubescentis and Ramulus et Folium Mussaendae Pubescentis powder join in culture matrix, both the idle waste problem of locality, Anji trimming leaf had been solved, also solve cultivation edible fungi raw materials shortage and cause rise in price problem, and finding through test, this kind of preparation more common edible fungi mouthfeel of edible fungi that obtain of cultivation is better, and fragrance is more strong, quality is more abundant.
As preferably, the water content of described cultivation matrix is between 55%-60%, and the edible fungi growing way between described water content is best, and eating effect is more preferably.
As preferably, the cultural method of described a kind of edible fungus culturing substrate, comprises the following steps: (1) weighs wood sawdust, Ramulus et Folium Mussaendae Pubescentis trimming leaf end, Ramulus et Folium Mussaendae Pubescentis powder, Testa Tritici, Gypsum Fibrosum powder and sucrose in proportion, stirs, precentagewise adds water mix homogeneously again, obtains mixture;
(2) take mixture described in step (1) to load in sterilizing bag, described mixture weight is controlled at 0.8-1.5kg, and compacting, then carry out sterilization treatment, obtain sterilizing bag;
(3) sterilizing bag described in step (2) is cooled down, more described sterilizing bag is worn dozen inoculation cave, then in inoculation cave, access Lentinus Edodes edible fungi original seed, cave will be inoculated by aseptic adhesive tape after inoculation and close, and obtain Lentinus Edodes edible fungi rod;
(4) Lentinus Edodes edible fungi rod described in step (3) is put in 22-26 DEG C of environment and cultivates, when mycelia color transition is sepia, sack described in step (2) is cut off, mycelia and culture matrix are carried out de-bag discharge, then concentrates and carry out booth cultivation.
Cultural method in this programme, operation is simple, easy to operate, and the edible fungi that obtains of cultivation is with effect of Ramulus et Folium Mussaendae Pubescentis, not only mouthfeel is good, and local flavor is good, can also draw through experimental data, the edible fungi survival rate height that this kind of cultivation method obtains, and grow fine, it is suitable for large-scale production.
As preferably, in described step (2), the weight of every bag of wet feed is 0.8-1kg, the weight of described every bag of cultivation matrix being contained within limits, and is that weight is in 0.8-1kg in order to the upgrowth situation according to edible fungi sets, growing fine of edible fungi, and culture matrix is without waste, it is possible to reach the effect checked and balanceed, and control the weight of every bag, the batch production of edible fungi can be controlled, it is simple to produce and management.
As preferably, the sterilizing methods in described step (2) is autoclaving, is first 0.5-1m by volume in step (2)3Sterilizing bag put into high-pressure sterilizing pot, be then forced into 1.5 atmospheric pressure and keep 40-60min, finally closing power supply, through 40-60min cooling decompression, sterilizing completes. Described step (2) carries out sterilizing be in order to prevent afterwards access edible fungi original seed time, edible fungi original seed is corroded by antibacterial, reduce edible fungi survival rate, thus productivity ratio can be reduced, increase production cost.
As preferably, sterilizing methods in described step (2) is sterilizing 12-16 hour at 100 DEG C, described step (2) carries out sterilizing be in order to prevent afterwards access edible fungi original seed time, edible fungi original seed is corroded by antibacterial, reduce the survival rate of edible fungi, thus productivity ratio can be reduced, increase production cost.
As preferably, in described step (3), inoculation cave diameter is 1.5cm, and the degree of depth is 2cm.The setting of described inoculation cave size, is that the size according to Inoculating needle sets, and the size inoculating cave also influences whether the growing way of mycelia, and the setting value of the hole size in inoculation cave is also the best hole value drawn according to experiment.
As preferably, the preparation method at described Ramulus et Folium Mussaendae Pubescentis trimming leaf end includes: take Ramulus et Folium Mussaendae Pubescentis trimming leaf, is dried by described Ramulus et Folium Mussaendae Pubescentis trimming leaf, with cutter, the Ramulus et Folium Mussaendae Pubescentis trimming leaf dried is sheared, make Ramulus et Folium Mussaendae Pubescentis trimming leaf between 0.5-2cm, make Ramulus et Folium Mussaendae Pubescentis trimming leaf end. Described Ramulus et Folium Mussaendae Pubescentis trimming leaf is contained within the composition that Ramulus et Folium Mussaendae Pubescentis has, and has effect similar with Ramulus et Folium Mussaendae Pubescentis, utilizes Ramulus et Folium Mussaendae Pubescentis trimming leaf, it is possible to reduce the consumption of Ramulus et Folium Mussaendae Pubescentis tea powder in scheme, reduces cost, and does not affect mouthfeel and the quality of edible fungi.
As preferably, the preparation method of described Ramulus et Folium Mussaendae Pubescentis powder includes: takes Ramulus et Folium Mussaendae Pubescentis and is put in exsiccator, at 80-100 DEG C of inner drying 10-40min, then take out and be rapidly cooled to-35 DEG C ~-10 DEG C, cooling time is 3-4 hour, then heat to room temperature, take every other day, the Ramulus et Folium Mussaendae Pubescentis handled well is put into grinder be ground, cross 200 mesh sieves, then Ramulus et Folium Mussaendae Pubescentis powder is made, the purpose being dried by Ramulus et Folium Mussaendae Pubescentis is to remove moisture, prevent mouldy, and it is to prevent Ramulus et Folium Mussaendae Pubescentis degeneration that dried Ramulus et Folium Mussaendae Pubescentis carries out rapidly cooling freezing, to ensure the functional component of Ramulus et Folium Mussaendae Pubescentis, have additional nutrients value for edible fungi, additionally, it is to make Ramulus et Folium Mussaendae Pubescentis tea powder better be integrated in other compositions that Ramulus et Folium Mussaendae Pubescentis tea powder is crossed 200 mesh sieves, make the edible fungus culture medium made more uniform, the edible fungi edibility of plantation is suitable, appearance is well-balanced.
In sum, the method have the advantages that
1. the cultivation matrix of a kind of edible fungi provided by the invention and cultural method, provides a kind of novel culturing raw material for cultivating edible fungi, adds the raw material resources of culturing edible fungus;
2. the cultivation matrix of a kind of edible fungi provided by the invention and cultural method, composition containing Anji Ramulus et Folium Mussaendae Pubescentis in cultivation matrix, for the edible nutritive value that adds of edible fungi, mouthfeel is more preferably;
3. the cultivation matrix of a kind of edible fungi provided by the invention and cultural method, cultural method is simple, and the survival rate cultivating edible fungi is high, excellent in efficiency;
4. the cultivation matrix of a kind of edible fungi provided by the invention and cultural method, the edible fungi quality that cultivation obtains is abundant, and edible fungi relatively before edible fungi more fragrant and sweet, mouthfeel is fresh and tender.
Detailed description of the invention
This specific embodiment is only explanation of the invention; it is not limitation of the present invention; the present embodiment can be made the amendment not having creative contribution as required by those skilled in the art after reading this specification, but as long as being affected by the protection of Patent Law in scope of the presently claimed invention.
The preparation method at Ramulus et Folium Mussaendae Pubescentis trimming leaf end includes: take Ramulus et Folium Mussaendae Pubescentis trimming leaf, is dried by Ramulus et Folium Mussaendae Pubescentis trimming leaf, with cutter, the Ramulus et Folium Mussaendae Pubescentis trimming leaf dried is sheared so that Ramulus et Folium Mussaendae Pubescentis trimming leaf, between 0.5-2cm, makes Ramulus et Folium Mussaendae Pubescentis trimming leaf end.
The preparation method one of Ramulus et Folium Mussaendae Pubescentis powder includes: takes Ramulus et Folium Mussaendae Pubescentis and is put in exsiccator, at 80 DEG C of inner drying 10min, then take out and be rapidly cooled to-35 DEG C, cooling time is 3 hours, then heat to room temperature, take every other day, the Ramulus et Folium Mussaendae Pubescentis handled well is put into grinder and is ground, cross 200 mesh sieves, then make Ramulus et Folium Mussaendae Pubescentis powder.
The preparation method two of Ramulus et Folium Mussaendae Pubescentis powder includes: takes Ramulus et Folium Mussaendae Pubescentis and is put in exsiccator, at 100 DEG C of inner drying 40min, then take out and be rapidly cooled to-10 DEG C, cooling time is 4 hours, then heat to room temperature, take every other day, the Ramulus et Folium Mussaendae Pubescentis handled well is put into grinder and is ground, cross 200 mesh sieves, then make Ramulus et Folium Mussaendae Pubescentis powder.
The preparation method three of Ramulus et Folium Mussaendae Pubescentis powder includes: takes Ramulus et Folium Mussaendae Pubescentis and is put in exsiccator, at 90 DEG C of inner drying 30min, then take out and be rapidly cooled to-20 DEG C, cooling time is 3.4 hours, then heat to room temperature, take every other day, the Ramulus et Folium Mussaendae Pubescentis handled well is put into grinder and is ground, cross 200 mesh sieves, then make Ramulus et Folium Mussaendae Pubescentis powder.
Formula 1: wood sawdust 58 weight portion, Ramulus et Folium Mussaendae Pubescentis trimming leaf end 15 weight portions, Ramulus et Folium Mussaendae Pubescentis powder 5 weight portion of preparation method one preparation of Ramulus et Folium Mussaendae Pubescentis powder, Testa Tritici 20 weight portion, Gypsum Fibrosum powder 1 weight portion, sucrose 1 weight portion.
Formula 2: wood sawdust 70 weight portion, Ramulus et Folium Mussaendae Pubescentis trimming leaf end 23 weight portions, Ramulus et Folium Mussaendae Pubescentis powder 7 weight portion of preparation method two preparation of Ramulus et Folium Mussaendae Pubescentis powder, Testa Tritici 20 weight portion, sucrose 1.5 weight portion, Gypsum Fibrosum powder 2 weight portion.
Formula 3: wood sawdust 15 weight portion, Ramulus et Folium Mussaendae Pubescentis trimming leaf end 4 weight portions, Ramulus et Folium Mussaendae Pubescentis powder 1 weight portion of preparation method three preparation of Ramulus et Folium Mussaendae Pubescentis powder, Testa Tritici 25 weight portion, sucrose 2 weight portion.
Formula 4: wood sawdust 10 weight portion, Ramulus et Folium Mussaendae Pubescentis trimming leaf end 50 weight portions, Testa Tritici 15 weight portion, sucrose 1 weight portion, Gypsum Fibrosum powder 3 weight portion.
Formula 5: wood sawdust 80 weight portion, Ramulus et Folium Mussaendae Pubescentis powder 7 weight portion, Testa Tritici 30 weight portion, sucrose 5 weight portion, Gypsum Fibrosum powder 5 weight portion.
Embodiment 1
The cultural method of a kind of edible fungus culturing substrate, comprises the following steps:
(1) weigh the wood sawdust in formula 1, Ramulus et Folium Mussaendae Pubescentis trimming leaf end, Ramulus et Folium Mussaendae Pubescentis powder, Testa Tritici, Gypsum Fibrosum powder and sucrose in proportion, stir, add the water mix homogeneously of gross mass 55%, obtain mixture;
(2) take mixture in step (1) and load in bag, control mixture wet feed weight at 1kg, and compacting, then carry out sterilization treatment, obtain sterilizing bag;
(3) sterilizing bag in step (2) is cooled down, then sterilizing bag is worn dozen inoculation cave, then in inoculation cave, access edible fungi original seed, cave will be inoculated by aseptic adhesive tape after inoculation and close, and obtain Lentinus Edodes edible fungi rod;
(4) Lentinus Edodes edible fungi rod in step (3) is put in 26 DEG C of environment and cultivates, when mycelia color transition is sepia, sack in step (2) is cut off, mycelia and culture matrix are carried out de-bag discharge, then concentrates and carry out booth cultivation.
Sterilizing methods in step (2) is autoclaving, is first 0.5-1m by volume in step (2)3Sterilizing bag put into high-pressure sterilizing pot, be then forced into 1.5 atmospheric pressure and keep 40min, finally closing power supply, decompression cooling, sterilizing completes.
In step (3), inoculation cave diameter is 1.5cm, and the degree of depth is 2cm.
Embodiment 2
The cultural method of edible fungus culturing substrate with embodiment 1, the difference is that:
Step (1) adds the water mix homogeneously of heavy amount 58% after weighing dispensing in formula 2 ratio, obtain mixture;
Step (2) controls mixture wet feed weight at 0.8kg, and compacting, then carries out sterilization treatment, obtains sterilizing bag;
Step (4) is to be put in Lentinus Edodes edible fungi rod in step (3) in 22 DEG C of environment to cultivate, and when mycelia color transition is sepia, is cut off by sack in step (2), mycelia and culture matrix carries out de-bag discharge, then concentrates and carry out booth cultivation.
Sterilizing methods in step (2) is autoclaving, is first 0.5-1m by volume in step (2)3Sterilizing bag put into high-pressure sterilizing pot, be then forced into 1.5 atmospheric pressure and keep 50min, finally closing power supply, decompression cooling, sterilizing completes.
Embodiment 3
The cultural method of edible fungus culturing substrate with embodiment 2, the difference is that:
Step (1) adds the water mix homogeneously of heavy amount 60% after weighing dispensing in formula 3 ratio, obtain mixture;
Step (2) controls mixture wet feed weight at 0.9kg, and compacting, then carries out sterilization treatment, obtains sterilizing bag;
Step (4) is to be put in Lentinus Edodes edible fungi rod in step (3) in 24 DEG C of environment to cultivate, and when mycelia color transition is sepia, is cut off by sack in step (2), mycelia and culture matrix carries out de-bag discharge, then concentrates and carry out booth cultivation.
Sterilizing methods in step (2) is autoclaving, is first 0.5-1m by volume in step (2)3Sterilizing bag put into high-pressure sterilizing pot, be then forced into 1.5 atmospheric pressure and keep 60min, finally closing power supply, decompression cooling, sterilizing completes.
Embodiment 4
The cultural method of edible fungus culturing substrate with embodiment 1, the difference is that: the sterilizing methods in step (2) is sterilizing 12 hours at 100 DEG C.
Embodiment 5
The cultural method of edible fungus culturing substrate with embodiment 2, the difference is that: the sterilizing methods in step (2) is sterilizing 14 hours at 100 DEG C.
Embodiment 6
The cultural method of edible fungus culturing substrate with embodiment 3, the difference is that: the sterilizing methods in step (2) is sterilizing 16 hours at 100 DEG C.
Comparative example 1
The cultural method of a kind of edible fungus culturing substrate, comprises the following steps:
(1) weigh the wood sawdust in formula 4, Ramulus et Folium Mussaendae Pubescentis trimming leaf end, Ramulus et Folium Mussaendae Pubescentis powder, Testa Tritici, Gypsum Fibrosum powder and sucrose in proportion, stir, add the water mix homogeneously of gross mass 70%, obtain mixture;
(2) take mixture described in step (1) and load in bag, control mixture weight at 1.5kg, and compacting, then carry out sterilization treatment, obtain sterilizing bag;
(3) sterilizing bag described in step (2) is cooled down, more described sterilizing bag is worn dozen inoculation cave, then in inoculation cave, access edible fungi original seed, cave will be inoculated by aseptic adhesive tape after inoculation and close, and obtain Lentinus Edodes edible fungi rod;
(4) Lentinus Edodes edible fungi rod described in step (3) is put in 20 DEG C of environment and cultivates, when mycelia color transition is sepia, sack described in step (2) is cut off, mycelia and culture matrix are carried out de-bag discharge, then concentrates and carry out booth cultivation.
Sterilizing methods in step (2) is autoclaving, is first 0.5-1m by volume in step (2)3Sterilizing bag put into high-pressure sterilizing pot, be then forced into 2 atmospheric pressure and keep 30min, finally closing power supply, decompression cooling, sterilizing completes.
In step (3), inoculation cave diameter is 2cm, and the degree of depth is 1.5cm.
Comparative example 2
The cultural method of edible fungus culturing substrate with embodiment 2, the difference is that:
Step (1) adds the water mix homogeneously of gross mass 50% after weighing dispensing in proportion by formula 5, obtain mixture;
Step (2) controls mixture wet feed weight at 0.5kg, and compacting, then carries out sterilization treatment, obtains sterilizing bag;
Lentinus Edodes edible fungi rod described in step (3) is put in 28 DEG C of environment and cultivates by step (4), when mycelia color transition is sepia, is cut off by sack described in step (2), mycelia and culture matrix carries out de-bag discharge, then concentrates and carry out booth cultivation.
Sterilizing methods in step (2) is sterilizing 10 hours at 100 DEG C.
Experimental result
Cultivation matrix culturing edible fungus is prepared respectively according to above-mentioned 5 kinds of embodiments, observed result after 2.5 months, and pluck edible, record result is as follows:
From the above experimental results, embodiment 1, embodiment 2, embodiment 3, embodiment 4, embodiment 5, the edible fungi that embodiment 6 is cultivated has good growing way, quality is abundant, head is uniform, and good mouthfeel, there is fragrance, but the edible fungi growth of comparative example 1 and comparative example 2 is bad, mouthfeel is not good yet, illustrate that the component proportion of the cultivation matrix of edible fungi and cultural method may decide that the upgrowth situation of edible fungi, and the component proportion of the cultivation matrix of the edible fungi of this programme and cultural method fit into the growth of edible fungi, and there is good effect.
Claims (9)
1. the cultivation matrix of an edible fungi, it is characterised in that: it includes following component and weight portion:
Wood sawdust 15-70 weight portion
Ramulus et Folium Mussaendae Pubescentis trimming leaf end 4-23 weight portion
Ramulus et Folium Mussaendae Pubescentis powder 1-7 weight portion
Testa Tritici 18-25 weight portion
Gypsum Fibrosum powder 0-2 weight portion
Sucrose 1-2 weight portion.
2. the cultivation matrix of a kind of edible fungi according to claim 1, it is characterised in that: described component also includes water, and the water content of described cultivation matrix is between 55%-60%.
3. the cultural method of an edible fungus culturing substrate according to claim 1 and 2, it is characterised in that: it comprises the following steps:
(1) weigh wood sawdust, Ramulus et Folium Mussaendae Pubescentis trimming leaf end, Ramulus et Folium Mussaendae Pubescentis powder, Testa Tritici, Gypsum Fibrosum powder and sucrose in proportion, stir, then precentagewise adds water mix homogeneously, obtains mixture;
(2) take mixture described in step (1) to load in sterilizing bag, described mixture weight is controlled at 0.8-1.5kg, and compacting, then carry out sterilization treatment, obtain sterilizing bag;
(3) sterilizing bag described in step (2) is cooled down, more described sterilizing bag is worn dozen inoculation cave, then in inoculation cave, access Lentinus Edodes edible fungi original seed, cave will be inoculated by aseptic adhesive tape after inoculation and close, and obtain Lentinus Edodes edible fungi rod;
(4) Lentinus Edodes edible fungi rod described in step (3) is put in 22-26 DEG C of environment and cultivates, when mycelia color transition is sepia, sack described in step (2) is cut off, mycelia and culture matrix are carried out de-bag discharge, then concentrates and carry out booth cultivation.
4. the cultural method of a kind of edible fungus culturing substrate according to claim 3, it is characterised in that: in described step (2), the weight of every bag of wet feed is 0.8-1kg.
5. the cultural method of a kind of edible fungus culturing substrate according to claim 4, it is characterised in that: the sterilizing methods in described step (2) is autoclaving, is first 0.5-1m by volume in step (2)3Sterilizing bag put into high-pressure sterilizing pot, be then forced into 1.5 atmospheric pressure and keep 40-60min, finally closing power supply, decompression cooling, sterilizing completes.
6. the cultural method of a kind of edible fungus culturing substrate according to claim 4, it is characterised in that: the sterilizing methods in described step (2) is sterilizing 12-16 hour at 100 DEG C.
7. the cultural method of a kind of edible fungus culturing substrate according to claim 3, it is characterised in that: in described step (3), inoculation cave diameter is 1.5cm, and the degree of depth is 2cm.
8. the cultural method of a kind of edible fungus culturing substrate according to claim 1, it is characterized in that: the preparation method at described Ramulus et Folium Mussaendae Pubescentis trimming leaf end includes: take Ramulus et Folium Mussaendae Pubescentis trimming leaf, described Ramulus et Folium Mussaendae Pubescentis trimming leaf is dried, with cutter, the Ramulus et Folium Mussaendae Pubescentis trimming leaf dried is sheared, make Ramulus et Folium Mussaendae Pubescentis trimming leaf between 0.5-2cm, make Ramulus et Folium Mussaendae Pubescentis trimming leaf end.
9. the cultural method of a kind of edible fungus culturing substrate according to claim 1, it is characterized in that: the preparation method of described Ramulus et Folium Mussaendae Pubescentis powder includes: take Ramulus et Folium Mussaendae Pubescentis and be put in exsiccator, at 80-100 DEG C of inner drying 10-40min, then taking out and be rapidly cooled to-35 DEG C ~-10 DEG C, cooling time is 3-4 hour, then heats to room temperature, take every other day, the Ramulus et Folium Mussaendae Pubescentis handled well is put into grinder be ground, cross 200 mesh sieves, then make Ramulus et Folium Mussaendae Pubescentis powder.
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