CN105661224A - 一种含有乳杆菌的饮料及其制备方法 - Google Patents
一种含有乳杆菌的饮料及其制备方法 Download PDFInfo
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- A—HUMAN NECESSITIES
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Abstract
本发明属于生物技术领域,公开了一种含有乳杆菌的饮料,其按照如下方法制备而得:1)制备提取物A;2)制备提取物B;3)制备提取物C,4)制备提取物D;5)制备提取物E;6)混匀消毒,以及7)发酵。本发明制备方法独特,不同的原料采用不同的处理方式,避免了单一处理方式造成的有效成分浪费,市场前景较好。
Description
技术领域
本发明属于生物技术领域,具体地涉及一种含有乳杆菌的饮料及其制备方法。
背景技术
饮料是指以水为基本原料,由不同的配方和制造工艺生产出来,供人或牲畜直接饮用的液体食品。饮料除提供水分外,由于在不同品种的饮料中含有不等量的糖、酸、乳、钠、脂肪、能量以及各种氨基酸、维生素、无机盐等营养成分,因此有一定的营养。中医对饮料的定义为:加工制造的供饮用的液体,如汽水、果子露、茶等。徐珂《清稗类钞·饮食·饮料食品》:“茶、汤、羹、浆、酪之属,皆饮料也。
目前市场上的饮料多种多样,其侧重点也有不同,有的仅具备解渴功能,有的具备一定的保健功能,例如针对缺钙人群的高钙奶以及针对便秘人群的有益菌饮料等等。糖尿病是由遗传因素、免疫功能紊乱、微生物感染及其毒素、自由基毒素、精神因素等等各种致病因子作用于机体导致胰岛功能减退、胰岛素抵抗等而引发的糖、蛋白质、脂肪、水和电解质等一系列代谢紊乱综合征,临床上以高血糖为主要特点,典型病例可出现多尿、多饮、多食、消瘦等表现,即“三多一少”症状,糖尿病(血糖)一旦控制不好会引发并发症,导致肾、眼、足等部位的衰竭病变,且无法治愈。长期服药对身体会有较大的毒副作用,并且影响胃、肝、肾的功能。如果不需要服药即可控制血糖,而且能够参与正常饮食,那么糖尿病患者将会大大减轻痛苦,并且生活质量大大提高。普通的饮料一般含有较多的糖份,一般不适合患者饮用,开发一种适合患者饮用的饮品尤为重要。
发明内容
为了克服现有技术的不足,本发明提供了一种含有乳杆菌的饮料,其制备方法独特,可有效地控制血糖和血脂。本发明还公开了上述饮料的制备方法。
为实现上述目的,本发明采用的技术方案如下:
一种含有乳杆菌的饮料,其按照如下方法制备而得:1)制备提取物A;2)制备提取物B;3)制备提取物C,4)制备提取物D;5)制备提取物E;6)混匀消毒,以及7)发酵。
具体地,其按照如下方法制备而得:
1)制备提取物A:将木瓜洗净后剖开去籽,切成碎块,风干,粉碎成粉末,然后放入体积分数为70%的乙醇中浸泡30分钟,以没过为准,减压旋转蒸发去除乙醇;随后紫外照射15min,再添加体积分数为85%的乙醇浸泡30min,然后1000转/min离心收集上清液,将上清液浓缩成密度为1.2g/ml的浸膏,80℃干燥后,粉碎成粉末,即得;
2)制备提取物B:将沙棘用高速组织捣碎机打浆,然后加水煮沸20min,用三层纱布过滤去渣,收集滤液即得;所述沙棘和水的质量比为1:3;
3)制备提取物C:将山楂去籽,切成片状,风干,然后添加五倍重量的水,武火煮沸,文火煮2小时,过滤收集滤液即得;
4)制备提取物D:将丹参、枸杞和牛蒡根按照2:2:1的重量比混合均匀,加5倍重量的水煎煮1小时,过滤收集滤液,用1mol/L的盐酸溶液调整滤液的pH为6.5,然后将滤液浓缩成密度为1.1g/ml的浸膏,80℃干燥后,粉碎成粉末即得;
5)制备提取物E:将粳米和薏苡仁按照2:1的重量比混合均匀,然后研磨成粉末状,过200目筛即得;
6)混匀消毒:将提取物A、提取物B、提取物C、提取物D、提取物E以及稳定剂投入到牛奶中,混合均匀,然后置于95℃消毒160秒,冷却降温到32℃,静置30min;所述提取物A、提取物B、提取物C、提取物D、提取物E、稳定剂以及牛奶的重量比为3-5:5-8:5-8:1-2:2-4:0.1-0.2:100-150;所述稳定剂由六偏磷酸钠、黄原胶以及海藻酸钠按照9:1:2的质量比混合而得;
7)发酵:加入保加利亚乳杆菌,发酵12小时,终止发酵,最后冷却降温到4℃,包装密封即得。
本发明还公开了上述饮料的制备方法。
本发明取得的有益效果主要包括:
本发明制备的木瓜提取物含有齐墩果酸、熊果酸以及维生素C等物质,能够有效地降低血糖和血脂,提高身体机能;本发明制备的沙棘提取物含有丰富的维生素以及沙棘黄酮,具备较好的降血糖降血脂等功效;山楂提取物中含有山楂黄酮等物质,可有效地降血糖;本发明通过添加多种名贵中药提取物,提高了饮品的药用价值;本发明含有乳杆菌,促进肠道吸收效果好;本发明制备的饮料可以有效地预防和治疗高血糖高血脂,无需服药或部分取代药物治疗,避免或减少了服用药物带来的毒副作用,并且还可以补充能量,一举两得。本发明制备方法独特,不同的原料采用不同的处理方式,避免了单一处理方式造成的营养成分浪费,可大规模工业化生产,前景较好。
具体实施方式
以下将采用具体的实施例来对本发明作进一步的解释,但是不应当看作是对本发明创新精神的限制。
实施例1
一种含有乳杆菌的饮料,其按照如下方法制备而得:
1)制备提取物A:将木瓜洗净后剖开去籽,切成碎块,风干,粉碎成粉末,然后放入体积分数为70%的乙醇中浸泡30分钟,以没过为准,减压旋转蒸发去除乙醇;随后紫外照射15min,再添加体积分数为85%的乙醇浸泡30min,然后1000转/min离心收集上清液,将上清液浓缩成密度为1.2g/ml的浸膏,80℃干燥后,粉碎成粉末,即得;
2)制备提取物B:将沙棘用高速组织捣碎机打浆,然后加水煮沸20min,用三层纱布过滤去渣,收集滤液即得;所述沙棘和水的质量比为1:3;
3)制备提取物C:将山楂去籽,切成片状,风干,然后添加五倍重量的水,武火煮沸,文火煮2小时,过滤收集滤液即得;
4)制备提取物D:将丹参、枸杞和牛蒡根按照2:2:1的重量比混合均匀,加5倍重量的水煎煮1小时,过滤收集滤液,用1mol/L的盐酸溶液调整滤液的pH为6.5,然后将滤液浓缩成密度为1.1g/ml的浸膏,80℃干燥后,粉碎成粉末即得;
5)制备提取物E:将粳米和薏苡仁按照2:1的重量比混合均匀,然后研磨成粉末状,过200目筛即得;
6)混匀消毒:将提取物A、提取物B、提取物C、提取物D、提取物E以及稳定剂投入到牛奶中,混合均匀,然后置于95℃消毒160秒,冷却降温到32℃,静置30min;所述提取物A、提取物B、提取物C、提取物D、提取物E、稳定剂以及牛奶的重量比为3:5:5:1:2:0.1:100;所述稳定剂由六偏磷酸钠、黄原胶以及海藻酸钠按照9:1:2的质量比混合而得;
7)发酵:加入保加利亚乳杆菌,发酵12小时,终止发酵,最后冷却降温到4℃,包装密封即得;其中,保加利亚乳杆菌的浓度为5×108个/ml,加入量与稳定剂重量相同。
实施例2
一种含有乳杆菌的饮料,其按照如下方法制备而得:
1)制备提取物A:将木瓜洗净后剖开去籽,切成碎块,风干,粉碎成粉末,然后放入体积分数为70%的乙醇中浸泡30分钟,以没过为准,减压旋转蒸发去除乙醇;随后紫外照射15min,再添加体积分数为85%的乙醇浸泡30min,然后1000转/min离心收集上清液,将上清液浓缩成密度为1.2g/ml的浸膏,80℃干燥后,粉碎成粉末,即得;
2)制备提取物B:将沙棘用高速组织捣碎机打浆,然后加水煮沸20min,用三层纱布过滤去渣,收集滤液即得;所述沙棘和水的质量比为1:3;
3)制备提取物C:将山楂去籽,切成片状,风干,然后添加五倍重量的水,武火煮沸,文火煮2小时,过滤收集滤液即得;
4)制备提取物D:将丹参、枸杞和牛蒡根按照2:2:1的重量比混合均匀,加5倍重量的水煎煮1小时,过滤收集滤液,用1mol/L的盐酸溶液调整滤液的pH为6.5,然后将滤液浓缩成密度为1.1g/ml的浸膏,80℃干燥后,粉碎成粉末即得;
5)制备提取物E:将粳米和薏苡仁按照2:1的重量比混合均匀,然后研磨成粉末状,过200目筛即得;
6)混匀消毒:将提取物A、提取物B、提取物C、提取物D、提取物E以及稳定剂投入到牛奶中,混合均匀,然后置于95℃消毒160秒,冷却降温到32℃,静置30min;所述提取物A、提取物B、提取物C、提取物D、提取物E、稳定剂以及牛奶的重量比为5:8:8:2:4:0.2:150;所述稳定剂由六偏磷酸钠、黄原胶以及海藻酸钠按照9:1:2的质量比混合而得;
7)发酵:加入保加利亚乳杆菌,发酵12小时,终止发酵,最后冷却降温到4℃,包装密封即得;其中,保加利亚乳杆菌的浓度为5×108个/ml,加入量与稳定剂重量相同。
实施例3
动物学实验
试验动物选择:选取健康的42日龄的SD大鼠50只,(200±10)g。大鼠在动物房适应喂养3天后,随机抽取10只作为正常对照组喂以普通维持饲料。高糖高脂饲料配方:大鼠基础饲料68%,蔗糖10%,蛋黄粉9%,猪油8%,胆固醇1%,胆盐0.2%,余量为其他物质。
实施方式:其余40只采用高糖高脂饲料喂养4周。4周后大鼠经禁食18h后,链脲佐菌素(STZ)静脉注射20mg/Kg,每两天一次,使用两次后,血糖血脂居高不下者为造模成功。选取造模成功的大鼠,随机分为3组,每组10只,即模型组(不施用任何药物)、本发明实施例1组和对照组:均施用格列奇特(10mg/kg),施用方式均为口服,早晚各一次,进食前半小时服用;本发明实施例1组额外每天施用实施例1制备的饮品50ml。连续服用10天后检测。
统计学分析:数据资料采用x±s表示,使用SPSS17.0统计软件进行数据分析,多组间比较采用方差分析,两组间比较采用t检验。P<0.05有统计学意义。
治疗效果:检测了空腹血糖(FBG)、总胆固醇(TC)、甘油三酯(TG)等生化指标,单位均为mmol/L,具体结果见表1。
表1
结论:通过上表可知,本发明实施例1制备的饮料能够有效地配合西药,显著降低对FBG、TC、TG。
实施例4
临床典型病例
巩某,男53岁,临沭县人,空腹血糖7.3mmol/L,餐后两小时血糖8.8mmol/L,总胆固醇6.5mmol/L,诊断为轻度高血糖高胆固醇,每天坚持饮用本发明实施例1制备的饮料,每次200ml,早晚各一次,一个月后,空腹血糖降至6.1mmol/L,餐后两小时血糖降至7.5mmol/L,总胆固醇为5.41mmol/L,坚持饮用本发明的饮料,上述指标保持正常,精神状态也明显好转。
上述采用具体实施例对本发明进行了详尽描述,但并非对本发明保护范围的限制,所属领域技术人员应该明白,在本发明的技术方案的基础上,本领域技术人员不需要付出创造性劳动即可做出的各种修改或变形仍在本发明的保护范围以内。
Claims (4)
1.一种含有乳杆菌的饮料,其按照如下方法制备而得:1)制备提取物A;2)制备提取物B;3)制备提取物C,4)制备提取物D;5)制备提取物E;6)混匀消毒,以及7)发酵。
2.根据权利要求1所述的饮料,其特征在于,所述饮料按照如下方法制备而得:
1)制备提取物A:将木瓜洗净后剖开去籽,切成碎块,风干,粉碎成粉末,然后放入体积分数为70%的乙醇中浸泡30分钟,以没过为准,减压旋转蒸发去除乙醇;随后紫外照射15min,再添加体积分数为85%的乙醇浸泡30min,然后1000转/min离心收集上清液,将上清液浓缩成密度为1.2g/ml的浸膏,80℃干燥后,粉碎成粉末,即得;
2)制备提取物B:将沙棘用高速组织捣碎机打浆,然后加水煮沸20min,用三层纱布过滤去渣,收集滤液即得;所述沙棘和水的质量比为1:3;
3)制备提取物C:将山楂去籽,切成片状,风干,然后添加五倍重量的水,武火煮沸,文火煮2小时,过滤收集滤液即得;
4)制备提取物D:将丹参、枸杞和牛蒡根按照2:2:1的重量比混合均匀,加5倍重量的水煎煮1小时,过滤收集滤液,用1mol/L的盐酸溶液调整滤液的pH为6.5,然后将滤液浓缩成密度为1.1g/ml的浸膏,80℃干燥后,粉碎成粉末即得;
5)制备提取物E:将粳米和薏苡仁按照2:1的重量比混合均匀,然后研磨成粉末状,过200目筛即得;
6)混匀消毒:将提取物A、提取物B、提取物C、提取物D、提取物E以及稳定剂投入到牛奶中,混合均匀,然后置于95℃消毒160秒,冷却降温到32℃,静置30min;
7)发酵:加入保加利亚乳杆菌,发酵12小时,终止发酵,最后冷却降温到4℃,包装密封即得。
3.根据权利要求2所述的饮料,其特征在于,所述提取物A、提取物B、提取物C、提取物D、提取物E、稳定剂以及牛奶的重量比为3-5:5-8:5-8:1-2:2-4:0.1-0.2:100-150。
4.根据权利要求2或3所述的饮料,其特征在于,所述稳定剂由六偏磷酸钠、黄原胶以及海藻酸钠按照9:1:2的质量比混合而得。
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