CN105641082A - Method for preparing anti-fatigue Chinese herb extract - Google Patents
Method for preparing anti-fatigue Chinese herb extract Download PDFInfo
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- CN105641082A CN105641082A CN201610045094.3A CN201610045094A CN105641082A CN 105641082 A CN105641082 A CN 105641082A CN 201610045094 A CN201610045094 A CN 201610045094A CN 105641082 A CN105641082 A CN 105641082A
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- Prior art keywords
- herba delphinii
- extract
- delphinii anthriscifolii
- anthriscifolii
- chinese medicine
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/71—Ranunculaceae (Buttercup family), e.g. larkspur, hepatica, hydrastis, columbine or goldenseal
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
Abstract
The invention discloses a method for preparing an anti-fatigue Chinese herb extract. The Chinese herb extract is a methanol extract of chervil larkspur herb. The Chinese herb extract prepared through the method has the effects of improving the exercise tolerance of an organism and reducing lactic acid in serum of the organism after exercises, and can well maintain the content of liver glucogen, and improve the adaptability of animals to exercise loads.
Description
The application is the divisional application of the applicant Cui Yinfang application for a patent for invention (denomination of invention is: the Chinese medicine extract of a kind of resisting fatigue and preparation technology thereof and application, and application number is: 2014103992846, and the applying date is: on August 14th, 2014) proposed.
Technical field
The invention belongs to Chinese crude drug extractive technique field, a kind of method relating to Chinese medicine extract preparing resisting fatigue.
Background technology
Along with the development of society, life stress, employment pressure increase, and increasing people feels tired in live and work, and fatigue is a kind of sub-health state, has a strong impact on quality of life. Clinical practice is a lot of in the medicine of alleviating physical fatigue and tonic, but these kinds have containing hormone, toxic and side effects is relatively big, and is inconvenient to take.
Herba Delphinii Anthriscifolii (DelphiniumanthriscifoliumHance), another name fish lamp is revived, Ranunculaceae Delphinium annual herb. High 30��70 centimetres; Blade Pedicellus et Pericarpium Trapae shape is avette or triangular shape is avette, and 2��3 times pinniform is totally cleaved; Raceme tool 2��15 flower, spends light greenish blue purple; Really. It is distributed in Guangdong, Guangxi, Guizhou, Hunan, Jiangxi, Fujian, Zhejiang, Jiangsu, Anhui, Henan, Southern Shanxi Province. It is born in hillside thick grass or the meadow, small stream limit on hills or low mountain between height above sea level 200-1200 rice. It is prone to plantation, has a very wide distribution, have higher medical value, be currently used for apoplexy and hemiplegia, rheumatism bones and muscles pain, there is no bibliographical information Herba Delphinii Anthriscifolii or its extract has effect of resisting fatigue.
Summary of the invention
It is an object of the invention to provide a kind of anti-fatigue Chinese medicine extract prepared using Herba Delphinii Anthriscifolii as raw material and preparation technology thereof and application.
In order to realize the purpose of the present invention, inventor, by lot of experiments research persistent exploration, is finally obtained following technical scheme:
The Chinese medicine extract of a kind of resisting fatigue, described Chinese medicine extract is the methanol extract of Herba Delphinii Anthriscifolii. It should be noted that Chinese crude drug Herba Delphinii Anthriscifolii of the present invention selects the herb of Ranunculaceae Genus Delphinium L. Herba Delphinii Anthriscifolii DelphiniumanthriscifoliumHance, it is possible to for the fresh goods just plucked, it is also possible to for the dry product after drying.
The preparation technology of the Chinese medicine extract of a kind of above-mentioned resisting fatigue, this technique comprises the steps: that extracting solution is condensed into thick paste, lyophilization, to obtain final product by the methanol solution lixiviate that Herba Delphinii Anthriscifolii volumetric concentration is 72-78%.
Preferably, the preparation technology of the Chinese medicine extract of resisting fatigue described above, wherein said leach step is: vibrate 0.5-1.5h by Herba Delphinii Anthriscifolii in the methanol solution that volumetric concentration is 72-78%, then at room temperature places 40-200h.
Preferably, the preparation technology of the Chinese medicine extract of resisting fatigue described above, wherein said concentration step is carried out below at 42 DEG C.
Preferably, the preparation technology of the Chinese medicine extract of resisting fatigue described above, wherein the consumption of methanol solution is 3-6 times (v/w) of Herba Delphinii Anthriscifolii quality.
Preferably, the preparation technology of the Chinese medicine extract of resisting fatigue described above, wherein this technique specifically includes following steps: Herba Delphinii Anthriscifolii is ground into powder, it is soaked in the methanol aqueous solution that concentration expressed in percentage by volume is 75% and carries out vibration 0.8-1.2h, it is filtered after at room temperature placing 60-96h subsequently, filtrate concentrating under reduced pressure under 40 DEG C of conditions is become thick paste, lyophilization, obtains the methanol extract of Herba Delphinii Anthriscifolii.
The present invention is observed the mice burden swimming time by design experiment, the content of lactic acid in blood plasma after detection mice burden swimming, it was demonstrated that Herba Delphinii Anthriscifolii extract have improve mouse movement endurance, reduce motion after Mouse Blood purify the blood the effect of middle lactic acid. Present invention test also confirms that after Herba Delphinii Anthriscifolii extract group mouse movement that liver glycogen content is apparently higher than blank group, serum urea nitrogen is significantly lower than blank group, illustrate that Herba Delphinii Anthriscifolii extract has and better maintain liver glycogen content, improve the animal adaptive capacity to sports load. Based on this result of study, further object is that a kind of pharmaceutical applications of offer, it may be assumed that the application in the medicine of preparation resisting fatigue of the above-mentioned Herba Delphinii Anthriscifolii extract.
Tired topmost performance is the decline of exercise tolerance, and exercise tolerance is the reflection the most direct index of organism fatigue, and the length of movement time can reflect the degree that animal movement is tired; And lactic acid is the product of sugar anerobic glycolysis in body, the accumulation of internal lactic acid, is the major reason causing sports fatigue, and the Chinese medicine extract that the present invention relates to have improve body movement endurance, reduce motion after body blood purify the blood the effect of middle lactic acid. It addition, sugar is the significant energy source of muscular tissue, glycogen depletion can affect motor capacity, and substantial amounts of research shows, along with being continuously increased of Body development consumption, human body, for maintaining blood sugar level, causes the minimizing of liver glycogen by employing liver glycogen. Therefore, the content of liver glycogen is the sensitive indicator of reflection degree of fatigue. After motion, liver glycogen content is more high, it was demonstrated that anti-fatigue effect is more good. When sugar, catabolism of fat can not obtain enough energy sources, protein catabolism strengthens and produces to increase with serum urea nitrogen, body serum urea nitrogen content increases with the increase of sports load, and the Herba Delphinii Anthriscifolii extract prepared by the present invention has better maintenance liver glycogen content, improve the animal adaptive capacity to sports load.
Detailed description of the invention
The following is the preparation embodiment of Herba Delphinii Anthriscifolii extract dry powder of the present invention and effect test example, the present invention will be further described. But, protection scope of the present invention is not limited to following example, and every technology realized based on foregoing of the present invention belongs to the scope of the present invention.
The preparation of embodiment 1 Herba Delphinii Anthriscifolii extract
1kg Herba Delphinii Anthriscifolii dry product is ground into powder, it is soaked in the methanol aqueous solution that 4L concentration expressed in percentage by volume is 75% and carries out vibration 1h, it is filtered after at room temperature placing 72h subsequently, filtrate concentrating under reduced pressure under 40 DEG C of conditions is become thick paste, lyophilization, it is ground into fine powder, crosses 100 mesh sieves, obtain Herba Delphinii Anthriscifolii extract dry powder.
The preparation of comparative example Herba Delphinii Anthriscifolii extract
1kg Herba Delphinii Anthriscifolii dry product is ground into powder, by the methanol aqueous solution heating and refluxing extraction that 4L concentration expressed in percentage by volume is 75% 2 times, merges 2 filtrates, filtrate concentrating under reduced pressure under 40 DEG C of conditions is become thick paste, lyophilization, is ground into fine powder, cross 100 mesh sieves, obtain Herba Delphinii Anthriscifolii extract dry powder.
Embodiment 2 Herba Delphinii Anthriscifolii extract is to mouse anti-reflecting fatigue experimental study
Healthy Kunming mouse 108, body weight l8g��22g, male and female half and half. After raising 7d in advance, it is randomly divided into following three groups, blank group, test A group, test B group, every group 36. And carry out following gavage process: with weight for benchmark, blank group gavage 0.9% normal saline, dosage is 0.15ml/10g; The Herba Delphinii Anthriscifolii extract of the test A group gavage embodiment of the present invention 1 preparation, Herba Delphinii Anthriscifolii extract prepared by test B group gavage comparative example, dosage is 1.72g/kg/d, and about 5 each gavages of every morning 9 and afternoon once, are administered 30d altogether. During experiment, animal freely ingests and drinks water, and last gavage is stopped eating evening before that day.
(1) exercise tolerance experiment
30min after last gavage, each group takes 12 mices respectively at random and is placed in swimming trunk went swimming. Depth of water 40cm, water temperature (25 �� 1) DEG C, from rat-tail point 1cm place, with the sheet lead of thin cotton cord system 5% body weight. Record mice enters water to head and all enters the time the lasting 8s of water can not emerge, i.e. the mice burden swimming time, investigate the impact on the mice burden swimming time of the Herba Delphinii Anthriscifolii extract water extract with this.
Herba Delphinii Anthriscifolii extract affects result in Table 1 to the mice burden swimming time. Result of the test according to table 1 can be seen that, the weight of each group mice is without significant difference, but the test A group mice burden swimming time is above matched group and test B group, and difference has extremely notable meaning (P < 0.01). This illustrates that the Herba Delphinii Anthriscifolii extract obtained by lixiviate has the effect extending the mice burden swimming time.
Table 1 respectively organizes Mouse Weight and walking weight load compares
Compare with blank group,��P < 0.05,���P < 0.01;
Compare with test B group,��P < 0.05,����P < 0.01.
(2) after mouse movement, liver glycogen and serum urea measure
30min after last gavage, each group takes 12 mices respectively at random, not swimming with a load attached to the body 90min in the water that temperature be (25 �� 1) DEG C, blood sampling after rest 60min. Pulling out eyeball to adopt whole blood (being not added with anticoagulant) and put 4 DEG C of refrigerators and be about 3h, after hemopexis, 2000r/min is centrifuged 15min, takes determination of serum serum urea content. Blood sampling puts to death animal after terminating immediately, takes liver and blots with filter paper after normal saline rinses, accurately weigh liver 100mg, measures liver glycogen content.
Herba Delphinii Anthriscifolii extract on Mouse Liver glycogen, serum urea content affect result in Table 2. Result of the test according to table 2 can be seen that, the liver glycogen level of test A group mice is significantly higher than matched group and test B group (P < 0.01), and serum urea level is substantially less than matched group and test B group (P < 0.05). This illustrate the Herba Delphinii Anthriscifolii extract obtained by lixiviate have promote mouse movement after liver glycogen content and effect of serum urea content after reducing mouse movement.
Table 2 respectively organizes liver glycogen and serum urea comparision contents after mouse movement
Compare with blank group,��P < 0.05,���P < 0.01;
Compare with test B group,��P < 0.05,����P < 0.01.
(3) mensuration of blood lactase acid after mouse movement
30min after last gavage, each group takes 12 mices respectively at random, and the 1st eye socket blood sampling is standby, then in the water that temperature be (25 �� 1) DEG C after not swimming with a load attached to the body 10min, the 2nd blood sampling immediately, take serum after being centrifuged standby. Measure twice Serum lactic acid content before and after swimming.
Serum lactic acid content is affected result in Table 3 by Herba Delphinii Anthriscifolii extract water extract.Result of the test according to table 3 can be seen that, by testing before mouse movement, post exercise Serum lactic acid content, it has been found that compared with matched group, the difference of blood lactase acid notable (P > 0.05) before the swimming of each administration group mice; But, after test A group mice swimming, 0min Serum lactic acid content is below matched group (P < 0.05) and test B group (P < 0.01). This illustrate the Herba Delphinii Anthriscifolii extract obtained by lixiviate have suppress mouse movement after effect of increasing of blood lactase acid.
After table 3 respectively organizes mouse movement, Serum lactic acid content compares
Compare with blank group,��P < 0.05,���P < 0.01;
Compare with test B group,��P < 0.05,����P < 0.01.
By testing discovery above, the Herba Delphinii Anthriscifolii extract obtained by lixiviate has the prolongation mice burden swimming time, promote after mouse movement liver glycogen content and reduce mouse movement after serum urea content, and effect that blood lactase acid increases after suppressing mouse movement. But the Herba Delphinii Anthriscifolii extract obtained by heating and refluxing extraction does not have above-mentioned biological activity, this is likely to relevant with the active component of resisting fatigue in high temperature destructible Herba Delphinii Anthriscifolii.
Claims (2)
1. the method for the Chinese medicine extract preparing resisting fatigue, it is characterised in that comprise the technical steps that: by the methanol solution lixiviate that Herba Delphinii Anthriscifolii volumetric concentration is 72-78%, extracting solution is condensed into thick paste, lyophilization, to obtain final product;
Described leach step is: by vibration 0.5-1.5h in methanol solution that Herba Delphinii Anthriscifolii volumetric concentration is 72-78%, then at room temperature place 40-200h;
Described concentration step is carried out below at 42 DEG C;
The consumption of described methanol solution is 3-6 times of v/w of Herba Delphinii Anthriscifolii quality.
2. the method for the Chinese medicine extract of preparation resisting fatigue according to claim 1, it is characterized in that: the preparation technology of the Chinese medicine extract of described resisting fatigue specifically includes following steps: Herba Delphinii Anthriscifolii is ground into powder, it is soaked in the methanol aqueous solution that concentration expressed in percentage by volume is 75% and carries out vibration 0.8-1.2h, it is filtered after at room temperature placing 60-96h subsequently, filtrate concentrating under reduced pressure under 40 DEG C of conditions is become thick paste, lyophilization, obtains the methanol extract of Herba Delphinii Anthriscifolii.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610045094.3A CN105641082A (en) | 2014-08-14 | 2014-08-14 | Method for preparing anti-fatigue Chinese herb extract |
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| CN201610045094.3A CN105641082A (en) | 2014-08-14 | 2014-08-14 | Method for preparing anti-fatigue Chinese herb extract |
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| CN201410399284.6A Division CN104173476B (en) | 2014-08-14 | 2014-08-14 | A kind of Chinese medicine extract of resisting fatigue and preparation technology thereof and application |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106688415A (en) * | 2016-11-30 | 2017-05-24 | 杨立新 | Method for cultivating selenium-zinc-enriched forest product |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106688415A (en) * | 2016-11-30 | 2017-05-24 | 杨立新 | Method for cultivating selenium-zinc-enriched forest product |
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Inventor after: Zheng Fang Inventor after: Shi Tingqi Inventor before: Shi Tingqi |
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Application publication date: 20160608 |