CN105613294A - Culture medium for improving stem section propagation coefficient of dendrobium officinale - Google Patents
Culture medium for improving stem section propagation coefficient of dendrobium officinale Download PDFInfo
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- CN105613294A CN105613294A CN201610034101.XA CN201610034101A CN105613294A CN 105613294 A CN105613294 A CN 105613294A CN 201610034101 A CN201610034101 A CN 201610034101A CN 105613294 A CN105613294 A CN 105613294A
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- culture medium
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
Abstract
The invention discloses a culture medium for improving a stem section propagation coefficient of dendrobium officinale. The culture medium consists of a basal culture medium and additives. By using the culture medium, the stem section propagation coefficient of the dendrobium officinale can be improved obviously, the culture period is shortened relatively, and the economic benefit can be remarkably improved.
Description
Technical field:
The invention belongs to biological field, in particular to a kind of culture medium improving Herba Dendrobii Stem section reproduction coefficient.
Background technology:
Herba Dendrobii (DendrobiumofficinaleKimuraetMigo), has another name called Herba hedyotis costatae (" Chinese Higher plant illustrated handbook "), for the perennial herbaceous plant that grows nonparasitically upon another plant of the orchid family Dendrobium. Herba Dendrobii produces the west and south, Anhui, southeastern Yunnan, Xining, Fujian, Eastern Part of Zhejiang Province, China, the northwestward, Guangxi and Sichuan. It is born on the rock that the mountain region half of height above sea level up to 1600 meters is dark and damp. The medicinal effects of Herba Dendrobii is mainly fresh or dry stem, records: Herba Dendrobii has reinforcing stomach reg fluid, effect of nourishing YIN and clearing away heat in 2010 editions pharmacopeia. The medicinal ingredient of Herba Dendrobii mainly has polysaccharide, alkaloid, bibenzyl material and luxuriant and rich with fragrance class material etc., polysaccharose substance can improve body immunity, luxuriant and rich with fragrance class material and bibenzyl material etc. have the effect suppressing kinds of tumor cells, and wherein polysaccharide is the topmost active ingredient generally acknowledged at present.
Digging wantonly artificial in recent years, adds that Herba Dendrobii natural propagation speed is very low, and Herba Dendrobii wild resource is endangered. At present, the method for artificial propagation Herba Dendrobii mainly has plant division, cuttage and tissue-culturing rapid propagation. When group training, can mainly there be seed, stem section, blade and young root etc. as outer implant. Wherein, when seed is as outer implant, the meristematic capacity of obtained callus and protocorm and quality are better, but the rate of vaccination of Herba Dendrobii is low, not easily obtains. So utilizing stem section to carry out numerous soon to supplement as the one meeting the market demand as outer implant, therefore find a kind of breeding coefficient that can improve Herba Dendrobii stem section and shorten the culture medium of cultivation cycle, can better utilize this resource of Herba Dendrobii.
Summary of the invention:
It is an object of the invention to provide a kind of culture medium improving Herba Dendrobii Stem section reproduction coefficient, the composition of this culture medium includes: basal medium and additive.
The component of described basal medium and content is:
KNO375-2950mg/L, NH4NO3103-1522mg/L, KH2PO418-410mg/L, MgSO4��7H2O240-750mg/L, CaCl2��2H2O144-462mg/L, KCl0-68mg/L, KI0.7-1.7mg/L, H3BO30.6-6.4mg/L, MnSO4��4H2O3-26mg/L, ZnSO4��7H2O1.4-8.7mg/L, Na2MoO4��2H2O0-0.3mg/L, CuSO4��5H2O0-0.35mg/L, CoCl2��6H2O0-0.03mg/L, Na2-EDTA0-37mg/L, FeSO4��7H2O24-29mg/L, inositol 0-105mg/L, glycine 0-2.8mg/L, VB10.2-12.0mg/L, VB60.3-1.2mg/L, nicotinic acid 0.4-1.4mg/L, 6-benzyladenine 1.0-3.0mg/L, naphthalene acetic acid 0.05-0.5mg/L, sucrose 20-30g/L, agar 4.0-7.0g/L.
Described additive is the mixture of murphy juice, bananas juice and Sucus Cocois, the total concentration of the former two is 20-300g/L, the addition volume of Sucus Cocois is 1-255mL/L, ratio M (Rhizoma Solani tuber osi): M (Fructus Musae) of mixture: V (Sucus Cocois)=5-100: 2-150: 1-50.
A kind of culture medium improving Herba Dendrobii Stem section reproduction coefficient of the present invention is decided to be A culture medium, and it is formulated as existing compound method, and difference is to replace distilled water to prepare with the juice extracted, and wherein the acquisition mode of additive is as follows:
1. murphy juice acquisition methods: weigh the Rhizoma Solani tuber osi of required grams, is cut into Semen phaseoli radiati size, puts in pot, adds the water of dose volume about 70%, boils 30 minutes, then by 8 layers of filtered through gauze and get final product.
2. bananas juice acquisition methods: weigh the Fructus Musae of required grams, Fructus Musae is pressed into pasty state, puts in pot, adds the water of dose volume about 70%, boils 30 minutes, then by 2 layers of filtered through gauze and get final product.
3. Sucus Cocois acquisition methods: directly measure volume required from Cortex cocois radicis.
Usefulness of the present invention: adopt this culture medium can improve Herba Dendrobii Stem section reproduction coefficient, and shorten subculture cycle, can provide a thinking for the imbalance between supply and demand and protection Herba Dendrobii wild resource alleviating Herba Dendrobii.
Detailed description of the invention
Embodiment 1
Take dendrobium officinale stem section, soak 5 minutes with 0.1% washing powder solution, flowing water rushes 1 hour, with 75% alcohol disinfecting 30 seconds on aseptic superclean bench, aseptic washing 3 times, sterilize 8 minutes in 0.10% mercuric chloride, aseptic washing 6 times, is cut into each axillalry bud one section with dissecting knife by the stem section after sterilizing, is inoculated in MS culture medium, another containing 6-BA1.0mg/L, NAA0.2mg/L, treated that axillalry bud extends after 20 days, after stem section restoration ecosystem, it is inoculated in A culture medium and grows, consisting of of A culture medium:
KNO375mg/L, NH4NO3103mg/L, KH2PO418mg/L, MgSO4��7H2O240mg/L, CaCl2��2H2O144mg/L, KCl1mg/L, KI0.7mg/L, H3BO30.6mg/L, MnSO4��4H2O3mg/L, ZnSO4��7H2O1.4mg/L, Na2MoO4��2H2O0.1mg/L, CuSO4��5H2O0.15mg/L, CoCl2��6H2O0.01mg/L, Na2-EDTA10mg/L, FeSO4��7H2O24mg/L, inositol 12mg/L, glycine 1.2mg/L, VB10.2mg/L, VB60.3mg/L, nicotinic acid 0.4mg/L, 6-benzyladenine 1.0mg/L, naphthalene acetic acid 0.05mg/L, sucrose 20g/L, agar 4.0g/L. It is 20g/L that added Rhizoma Solani tuber osi and Fructus Musae extract mixture total concentration, additive ratio M (Rhizoma Solani tuber osi): M (Fructus Musae): V (Sucus Cocois)=5: 2: 1. Cultivation temperature 25 �� 1 DEG C, illumination every day 12 hours, intensity of illumination 1000-3000lux, cultivation cycle is 50 days. Adding up Herba Dendrobii Multiple Buds situation after 50 days, growth coefficient reaches 6-9, and the culture medium not adding additive improves 3-5 times.
Embodiment 2
Take dendrobium officinale stem section, soak 5 minutes with 0.1% washing powder solution, flowing water rushes 1 hour, with 75% alcohol disinfecting 30 seconds on aseptic superclean bench, aseptic washing 3 times, sterilize 8 minutes in 0.10% mercuric chloride, aseptic washing 6 times, is cut into each axillalry bud one section with dissecting knife by the stem section after sterilizing, is inoculated in MS culture medium, another containing 6-BA1.0mg/L, NAA0.2mg/L, treated that axillalry bud extends after 20 days, after stem section restoration ecosystem, it is inoculated in A culture medium and grows, consisting of of A culture medium:
KNO380mg/L, NH4NO3113mg/L, KH2PO419mg/L, MgSO4��7H2O250mg/L, CaCl2��2H2O154mg/L, KCl23mg/L, KI0.9mg/L, H3BO30.76mg/L, MnSO4��4H2O5mg/L, ZnSO4��7H2O1.6mg/L, Na2MoO4��2H2O0.1mg/L, CuSO4��5H2O0.25mg/L, CoCl2��6H2O0.01mg/L, Na2-EDTA12mg/L, FeSO4��7H2O25mg/L, inositol 15mg/L, glycine 1.4mg/L, VB10.3mg/L, VB60.4mg/L, nicotinic acid 0.5mg/L, 6-benzyladenine 1.2mg/L, naphthalene acetic acid 0.15mg/L, sucrose 25g/L, agar 4.0g/L. It is 50g/L that added Rhizoma Solani tuber osi and Fructus Musae extract mixture total concentration, additive ratio M (Rhizoma Solani tuber osi): M (Fructus Musae): V (Sucus Cocois)=15: 42: 19. Cultivation temperature 25 �� 1 DEG C, illumination every day 12 hours, intensity of illumination 1000-3000lux, cultivation cycle is 50 days. Adding up Herba Dendrobii Multiple Buds situation after 50 days, growth coefficient reaches 6-9, and the culture medium not adding additive improves 3-5 times.
Embodiment 3
Take dendrobium officinale stem section, soak 5 minutes with 0.1% washing powder solution, flowing water rushes 1 hour, with 75% alcohol disinfecting 30 seconds on aseptic superclean bench, aseptic washing 3 times, sterilize 8 minutes in 0.10% mercuric chloride, aseptic washing 6 times, is cut into each axillalry bud one section with dissecting knife by the stem section after sterilizing, is inoculated in MS culture medium, another containing 6-BA1.0mg/L, NAA0.2mg/L, treated that axillalry bud extends after 20 days, after stem section restoration ecosystem, it is inoculated in A culture medium and grows, consisting of of A culture medium:
KNO31000mg/L, NH4NO31113mg/L, KH2PO4190mg/L, MgSO4��7H2O550mg/L, CaCl2��2H2O254mg/L, KCl43mg/L, KI0.9mg/L, H3BO33.6mg/L, MnSO4��4H2O25mg/L, ZnSO4��7H2O8.6mg/L, Na2MoO4��2H2O0.3mg/L, CuSO4��5H2O0.25mg/L, CoCl2��6H2O0.03mg/L, Na2-EDTA32mg/L, FeSO4��7H2O26mg/L, inositol 95mg/L, glycine 2.4mg/L, VB110mg/L, VB60.9mg/L, nicotinic acid 0.7mg/L, 6-benzyladenine 1.8mg/L, naphthalene acetic acid 0.25mg/L, sucrose 25g/L, agar 5.0g/L. It is 100g/L that added Rhizoma Solani tuber osi and Fructus Musae extract mixture total concentration, additive ratio M (Rhizoma Solani tuber osi): M (Fructus Musae): V (Sucus Cocois)=25: 65: 34. Cultivation temperature 25 �� 1 DEG C, illumination every day 12 hours, intensity of illumination 1000-3000lux, cultivation cycle is 50 days. Adding up Herba Dendrobii Multiple Buds situation after 50 days, growth coefficient reaches 6-9, and the culture medium not adding additive improves 3-5 times.
Embodiment 4
Take dendrobium officinale stem section, soak 5 minutes with 0.1% washing powder solution, flowing water rushes 1 hour, with 75% alcohol disinfecting 30 seconds on aseptic superclean bench, aseptic washing 3 times, sterilize 8 minutes in 0.10% mercuric chloride, aseptic washing 6 times, is cut into each axillalry bud one section with dissecting knife by the stem section after sterilizing, is inoculated in MS culture medium, another containing 6-BA1.0mg/L, NAA0.2mg/L, treated that axillalry bud extends after 20 days, after stem section restoration ecosystem, it is inoculated in A culture medium and grows, consisting of of A culture medium:
KNO32300mg/L, NH4NO31513mg/L, KH2PO4290mg/L, MgSO4��7H2O600mg/L, CaCl2��2H2O354mg/L, KCl53mg/L, KI1.2mg/L, H3BO34.6mg/L, MnSO4��4H2O20mg/L, ZnSO4��7H2O7.6mg/L, Na2MoO4��2H2O0.2mg/L, CuSO4��5H2O0.35mg/L, CoCl2��6H2O0.03mg/L, Na2-EDTA35mg/L, FeSO4��7H2O28mg/L, inositol 105mg/L, glycine 2.5mg/L, VB111mg/L, VB60.7mg/L, nicotinic acid 0.9mg/L, 6-benzyladenine 2.4mg/L, naphthalene acetic acid 0.35mg/L, sucrose 30g/L, agar 6.0g/L. It is 200g/L that added Rhizoma Solani tuber osi and Fructus Musae extract mixture total concentration, additive ratio M (Rhizoma Solani tuber osi): M (Fructus Musae): V (Sucus Cocois)=78: 43: 5. Cultivation temperature 25 �� 1 DEG C, illumination every day 12 hours, intensity of illumination 1000-3000lux, cultivation cycle is 50 days. Adding up Herba Dendrobii Multiple Buds situation after 50 days, growth coefficient reaches 6-9, and the culture medium not adding additive improves 3-5 times.
Embodiment 5
Take dendrobium officinale stem section, soak 5 minutes with 0.1% washing powder solution, flowing water rushes 1 hour, with 75% alcohol disinfecting 30 seconds on aseptic superclean bench, aseptic washing 3 times, sterilize 8 minutes in 0.10% mercuric chloride, aseptic washing 6 times, is cut into each axillalry bud one section with dissecting knife by the stem section after sterilizing, is inoculated in MS culture medium, another containing 6-BA1.0mg/L, NAA0.2mg/L, treated that axillalry bud extends after 20 days, after stem section restoration ecosystem, it is inoculated in A culture medium and grows, consisting of of A culture medium:
KNO32500mg/L, NH4NO31450mg/L, KH2PO4400mg/L, MgSO4��7H2O700mg/L, CaCl2��2H2O154mg/L, KCl5mg/L, KI1.2mg/L, H3BO33.6mg/L, MnSO4��4H2O12mg/L, ZnSO4��7H2O4.6mg/L, Na2MoO4��2H2O0.1mg/L, CuSO4��5H2O0.15mg/L, CoCl2��6H2O0.02mg/L, Na2-EDTA25mg/L, FeSO4��7H2O28mg/L, inositol 55mg/L, glycine 2.1mg/L, VB11.1mg/L, VB60.7mg/L, nicotinic acid 0.8mg/L, 6-benzyladenine 3.0mg/L, naphthalene acetic acid 0.4mg/L, sucrose 30g/L, agar 6.0g/L. It is 250g/L that added Rhizoma Solani tuber osi and Fructus Musae extract mixture total concentration, additive ratio M (Rhizoma Solani tuber osi): M (Fructus Musae): V (Sucus Cocois)=55: 93: 50. Cultivation temperature 25 �� 1 DEG C, illumination every day 12 hours, intensity of illumination 1000-3000lux, cultivation cycle is 50 days. Adding up Herba Dendrobii Multiple Buds situation after 50, growth coefficient reaches 6-9, and the culture medium not adding additive improves 3-5 times.
Embodiment 6
Take dendrobium officinale stem section, soak 5 minutes with 0.1% washing powder solution, flowing water rushes 1 hour, with 75% alcohol disinfecting 30 seconds on aseptic superclean bench, aseptic washing 3 times, sterilize 8 minutes in 0.10% mercuric chloride, aseptic washing 6 times, is cut into each axillalry bud one section with dissecting knife by the stem section after sterilizing, is inoculated in MS culture medium, another containing 6-BA1.0mg/L, NAA0.2mg/L, treated that axillalry bud extends after 20 days, after stem section restoration ecosystem, it is inoculated in A culture medium and grows, consisting of of A culture medium:
KNO32911mg/L, NH4NO31031mg/L, KH2PO4138mg/L, MgSO4��7H2O249mg/L, CaCl2��2H2O181mg/L, KCl19mg/L, KI0.7mg/L, H3BO35.1mg/L, MnSO4��4H2O17mg/L, ZnSO4��7H2O6.4mg/L, Na2MoO4��2H2O0.1mg/L, CuSO4��5H2O0.11mg/L, CoCl2��6H2O0.01mg/L, Na2-EDTA13mg/L, FeSO4��7H2O25mg/L, inositol 102mg/L, glycine 1.5mg/L, VB12.2mg/L, VB60.7mg/L, nicotinic acid 0.7mg/L, 6-benzyladenine 1.3mg/L, naphthalene acetic acid 0.15mg/L, sucrose 20g/L, agar 4.0g/L. It is 50g/L that added Rhizoma Solani tuber osi and Fructus Musae extract mixture total concentration, the two ratio M (Rhizoma Solani tuber osi): M (Fructus Musae)=5: 2. Cultivation temperature 25 �� 1 DEG C, illumination every day 12 hours, intensity of illumination 1000-3000lux, cultivation cycle is 50 days. Adding up Herba Dendrobii Multiple Buds situation after 50 days, growth coefficient is only 3-5, all adds fashionable growth coefficient lower than three.
Embodiment 7
Take dendrobium officinale stem section, soak 5 minutes with 0.1% washing powder solution, flowing water rushes 1 hour, with 75% alcohol disinfecting 30 seconds on aseptic superclean bench, aseptic washing 3 times, sterilize 8 minutes in 0.10% mercuric chloride, aseptic washing 6 times, is cut into each axillalry bud one section with dissecting knife by the stem section after sterilizing, is inoculated in MS culture medium, another containing 6-BA1.0mg/L, NAA0.2mg/L, treated that axillalry bud extends after 20 days, after stem section restoration ecosystem, it is inoculated in A culture medium and grows, consisting of of A culture medium:
KNO31075mg/L, NH4NO31331mg/L, KH2PO4320mg/L, MgSO4��7H2O511mg/L, CaCl2��2H2O244mg/L, KCl19mg/L, KI0.7mg/L, H3BO30.6mg/L, MnSO4��4H2O11mg/L, ZnSO4��7H2O5.4mg/L, Na2MoO4��2H2O0.1mg/L, CuSO4��5H2O0.25mg/L, CoCl2��6H2O0.01mg/L, Na2-EDTA18mg/L, FeSO4��7H2O27mg/L, inositol 72mg/L, glycine 1.4mg/L, VB15.2mg/L, VB60.5mg/L, nicotinic acid 1.4mg/L, 6-benzyladenine 1.4mg/L, naphthalene acetic acid 0.16mg/L, sucrose 25g/L, agar 4.5g/L. Adding Rhizoma Solani tuber osi extracting solution, concentration is 120g/L. Cultivation temperature 25 �� 1 DEG C, illumination every day 12 hours, intensity of illumination 1000-3000lux, cultivation cycle is 50 days. Adding up Herba Dendrobii Multiple Buds situation after 50 days, growth coefficient is only 3-5.
Embodiment 8
Take dendrobium officinale stem section, soak 5 minutes with 0.1% washing powder solution, flowing water rushes 1 hour, with 75% alcohol disinfecting 30 seconds on aseptic superclean bench, aseptic washing 3 times, sterilize 8 minutes in 0.10% mercuric chloride, aseptic washing 6 times, is cut into each axillalry bud one section with dissecting knife by the stem section after sterilizing, is inoculated in MS culture medium, another containing 6-BA1.0mg/L, NAA0.2mg/L, treated that axillalry bud extends after 20 days, after stem section restoration ecosystem, it is inoculated in A culture medium and grows, consisting of of A culture medium:
KNO31135mg/L, NH4NO31233mg/L, KH2PO4101mg/L, MgSO4��7H2O711mg/L, CaCl2��2H2O417mg/L, KI0.7mg/L, H3BO31.9mg/L, MnSO4��4H2O11mg/L, ZnSO4��7H2O6.1mg/L, Na2MoO4��2H2O0.2mg/L, CuSO4��5H2O0.25mg/L, CoCl2��6H2O0.01mg/L, Na2-EDTA11mg/L, FeSO4��7H2O27mg/L, inositol 99mg/L, glycine 2.3mg/L, VB110mg/L, VB60.4mg/L, nicotinic acid 0.9mg/L, 6-benzyladenine 2.0mg/L, naphthalene acetic acid 0.3mg/L, sucrose 30g/L, agar 6.0g/L. Adding Fructus Musae extracting solution, concentration is 80g/L. Cultivation temperature 25 �� 1 DEG C, illumination every day 12 hours, intensity of illumination 1000-3000lux, cultivation cycle is 50 days. Adding up Herba Dendrobii Multiple Buds situation after 50 days, growth coefficient is only 3-5.
Embodiment 9
Take dendrobium officinale stem section, soak 5 minutes with 0.1% washing powder solution, flowing water rushes 1 hour, with 75% alcohol disinfecting 30 seconds on aseptic superclean bench, aseptic washing 3 times, sterilize 8 minutes in 0.10% mercuric chloride, aseptic washing 6 times, is cut into each axillalry bud one section with dissecting knife by the stem section after sterilizing, is inoculated in MS culture medium, another containing 6-BA1.0mg/L, NAA0.2mg/L, treated that axillalry bud extends after 20 days, after stem section restoration ecosystem, it is inoculated in A culture medium and grows, consisting of of A culture medium:
KNO32177mg/L, NH4NO31111mg/L, KH2PO4330mg/L, MgSO4��7H2O511mg/L, CaCl2��2H2O279mg/L, KI1.7mg/L, H3BO36.1mg/L, MnSO4��4H2O21mg/L, ZnSO4��7H2O7.1mg/L, Na2MoO4��2H2O0.2mg/L, CuSO4��5H2O0.05mg/L, CoCl2��6H2O0.02mg/L, Na2-EDTA31mg/L, FeSO4��7H2O27mg/L, inositol 97mg/L, glycine 2.4mg/L, VB16mg/L, VB60.6mg/L, nicotinic acid 1.1mg/L, 6-benzyladenine 2.2mg/L, naphthalene acetic acid 0.5mg/L, sucrose 30g/L, agar 6.5g/L. Adding coconut juice, volume is 100mL/L. Cultivation temperature 25 �� 1 DEG C, illumination every day 12 hours, intensity of illumination 1000-3000lux, cultivation cycle is 50 days. Adding up Herba Dendrobii Multiple Buds situation after 50 days, growth coefficient is only 3-5.
Claims (3)
1. the culture medium improving Herba Dendrobii Stem section reproduction coefficient, it is characterised in that the composition of this culture medium includes: basal medium and additive.
2. a kind of culture medium improving Herba Dendrobii Stem section reproduction coefficient as claimed in claim 1, it is characterised in that the component of described basal medium and content is:
KNO375-2950mg/L, NH4NO3103-1522mg/L, KH2PO418-410mg/L, MgSO4��7H2O240-750mg/L, CaCl2��2H2O144-462mg/L, KCl0-68mg/L, KI0.7-1.7mg/L, H3BO30.6-6.4mg/L, MnSO4��4H2O3-26mg/L, ZnSO4��7H2O1.4-8.7mg/L, Na2MoO4��2H2O0-0.3mg/L, CuSO4��5H2O0-0.35mg/L, CoCl2��6H2O0-0.03mg/L, Na2-EDTA0-37mg/L, FeSO4 7H2O24-29mg/L, inositol 0-105mg/L, glycine 0-2.8mg/L, VB10.2-12.0mg/L, VB60.3-1.2mg/L, nicotinic acid 0.4-1.4mg/L, 6-benzyladenine 1.0-3.0mg/L, naphthalene acetic acid 0.05-0.5mg/L, sucrose 20-30g/L, agar 4.0-7.0g/L.
3. a kind of culture medium improving Herba Dendrobii Stem section reproduction coefficient as claimed in claim 1, it is characterized in that, described additive is the mixture of murphy juice, bananas juice and Sucus Cocois, the total concentration of the former two is 20-300g/L, the addition volume of Sucus Cocois is 1-255mL/L, ratio M (Rhizoma Solani tuber osi): M (Fructus Musae) of mixture: V (Sucus Cocois)=5-100: 2-150: 1-50.
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| CN106993536A (en) * | 2017-05-09 | 2017-08-01 | 中国药科大学 | A kind of culture medium for improving dendrobium candidum protocorm breeding coefficient |
| CN110291987A (en) * | 2019-06-20 | 2019-10-01 | 成都师范学院 | A kind of Seeds of Dendrobium Candidum culture medium adding natural phytohormone |
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| CN104996301A (en) * | 2015-08-06 | 2015-10-28 | 云南省农业科学院花卉研究所 | Rapid propagation method for holcoglossum flavescens |
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| CN104996301A (en) * | 2015-08-06 | 2015-10-28 | 云南省农业科学院花卉研究所 | Rapid propagation method for holcoglossum flavescens |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106993536A (en) * | 2017-05-09 | 2017-08-01 | 中国药科大学 | A kind of culture medium for improving dendrobium candidum protocorm breeding coefficient |
| CN110291987A (en) * | 2019-06-20 | 2019-10-01 | 成都师范学院 | A kind of Seeds of Dendrobium Candidum culture medium adding natural phytohormone |
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