CN106719951A - A kind of preparation method of the biological agent for preventing and treating tomato seedling damping-off - Google Patents
A kind of preparation method of the biological agent for preventing and treating tomato seedling damping-off Download PDFInfo
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- CN106719951A CN106719951A CN201611074563.0A CN201611074563A CN106719951A CN 106719951 A CN106719951 A CN 106719951A CN 201611074563 A CN201611074563 A CN 201611074563A CN 106719951 A CN106719951 A CN 106719951A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/02—Sulfur; Selenium; Tellurium; Compounds thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
- A01N65/48—Zingiberaceae [Ginger family], e.g. ginger or galangal
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Abstract
The present invention relates to a kind of preparation method of the biological agent for preventing and treating tomato seedling damping-off, including following preparation process：Honegsukle flower P.E, dandelion extract, Folium Artemisiae Argyi extract, Dysosma versipellis extract solution and garlic P.E are sequentially prepared first；Then it is 23 32% according to the mass percent of Honegsukle flower P.E, the mass percent of dandelion extract is 18 25%, the mass percent of Folium Artemisiae Argyi extract is 8 15%, the mass percent of Dysosma versipellis extract solution is 10 20%, the mass percent of garlic P.E is 8 15%, and the mass percent of Cosan is 15 23%；Heating, insulation, obtain mixture I after Honegsukle flower P.E, dandelion extract, Folium Artemisiae Argyi extract and garlic P.E are mixed；Add Cosan to stir in mixture I, be eventually adding after Dysosma versipellis extract solution is well mixed and prepare the biological agent of preventing and treating tomato seedling damping-off.Biological agent of the present invention has preferable prevention effect to tomato seedling damping-off.
Description
Technical field
The present invention relates to a kind of biological agent, specifically a kind of biological agent for preventing and treating tomato seedling damping-off and its preparation
Method.
Background technology
Tomato alias tomato, tomato, ancient name June persimmon, report of good news ternary.In Peru and Mexico, initially it is referred to as
" wolf peach ".Fruits nutrition enriches, and has flavour.Can eat raw, cook, being processed into catsup, juice or whole fruit canning.Tomato
It is that one of fruit and vegetable the most universal are cultivated in the whole world.The U.S., the Soviet Union, Italy and China are major producing country.In Europe, the United States continent
National, Chinese and Japan have large area greenhouse, vinyl house and other protecting field facility cultivations.Generally plant all over China.
Cultivated area still is continuing to expand.
The tomato seedling damping-off multiple nursery middle and later periods being born in after true leaf launches, dead seedling is slower.First there is ellipse in seedling base portion
Shape brown scab, ground blade noon on daytime wilts, and morning and evening can recover normal, gradually expand with scab, be recessed,
Around stem one week, basal part of stem discoloration drying shrinkage, sick seedling is no longer recovered after wilting, until withered.Now seedling stem has begun to wooden
Change, sick seedling can not fall uprightly, referred to as damping-off.When moist, the visible sparse mould layer of filbert spider reticulation near sick seedling,
Later stage forms sclerotium.This sick identification feature is seedling basal part of stem drying shrinkage, upright withered.
Rhizoctonia solani cause of disease belongs to the Rhizoctonia solani Kuhn of Deuteromycotina, mainly in forms such as mycelium and sclerotium in soil
In survive the winter.Found the general 2-3 that can be survived in soil of withered sclerotium.Germ is mainly by the side such as rainwater, flowing water, fertilising and farming
Formula its spread in china.In 15-21 DEG C of soil temperature, soil easily induces the disease under the conditions of gluing weight, acid soil, continuous cropping etc..
The content of the invention
The present invention seeks to the deficiency for solution above-mentioned technical problem, there is provided a kind of biological agent of preventing and treating tomato seedling damping-off
And preparation method thereof, it has preferable prevention effect to tomato seedling damping-off.
A kind of preparation method of the biological agent for preventing and treating tomato seedling damping-off, including following preparation process：
The preparation of step one, Honegsukle flower P.E：Extracting honeysuckle 280g, removes impurity, after cleaning, drying, adds 1L water, heating
To after seething with excitement, slow fire boils truce in 30 minutes, and after filtering, gained supernatant is Honegsukle flower P.E, is cooled to room temperature
It is standby；
The preparation of step 2, dandelion extract：Dandelion complete stool 120g is taken, is cleaned, dried, crushed, be subsequently adding 1L
Petroleum ether immersion extracted within 48 hours, Buchner funnel suction filtration, collect filtrate；Petroleum ether is reclaimed in 55~65 DEG C of vacuum distillations,
Add the petroleum ether of recovery to soak in filter residue to be extracted, repeat extraction 4~5 times and reclaim filtrate；Suction filtration after repeatedly extracting
The filtrate decompression distillation and concentration for obtaining, obtains mixture, as dandelion extract, standby；
The preparation of step 3, Folium Artemisiae Argyi extract：Folium artemisiae argyi 150g is taken, impurity is removed, cleaned, add 1L water, be heated to 5 points after boiling
Clock, the ginger splices for adding 5g fresh, re-modulation slow fire boils truce in 30 minutes, and after filtering, gained supernatant is Folium Artemisiae Argyi extract,
Room temperature is cooled to, it is standby；
The preparation of step 4, Dysosma versipellis extract solution：500 grams of fresh Dysosma versipellis is taken to mash, after adding 1L water to soak 6 hours, filtering,
Gained supernatant is Dysosma versipellis extract solution, is placed in 4 DEG C of refrigerators and saves backup；
The preparation of step 5, garlic P.E：Choose fresh garlic, clean, dry after weigh 300g, be put in volumetric concentration
The min of surface sterilization 5 is carried out in 75% ethanol, with being placed in tissue refiner after aseptic water washing 3 times, add 100 mL without
Bacterium water, is homogenized 5 min and is made into pasty state, then 4 DEG C of temperature, rotating speed 10000r/ in centrifuge with the rotating speed of 5000r/min
10min is centrifuged under conditions of min, gained supernatant is to obtain garlic P.E, is placed in 4 DEG C of refrigerators and saves backup；
Step 6, it is 23-32% according to the mass percent of Honegsukle flower P.E, the mass percent of dandelion extract is 18-
25%, the mass percent of Folium Artemisiae Argyi extract is 8-15%, and the mass percent of Dysosma versipellis extract solution is 10-20%, garlic P.E
Mass percent be 8-15%, the mass percent of Cosan is 15-23%, takes the honeysuckle prepared by above-mentioned steps and extracts
Thing, dandelion extract, Folium Artemisiae Argyi extract, Dysosma versipellis extract solution, garlic P.E and the Cosan for preparing in addition, it is standby；
Heated after the mixing of step 7, the Honegsukle flower P.E that will be taken, dandelion extract, Folium Artemisiae Argyi extract and garlic P.E
17-19 hours is incubated to 45-60 DEG C, mixture I is obtained；Then taken Cosan is added to stir in mixture I, most
The biological agent for taking and preventing and treating tomato seedling damping-off being prepared after Dysosma versipellis extract solution is well mixed is added afterwards.
Institute extracting honeysuckle extract, dandelion extract, Folium Artemisiae Argyi extract, Dysosma versipellis extract solution, garlic P.E and sulphur
The mass percent of sulphur powder is preferably：Honegsukle flower P.E 25%, dandelion extract 25%, Folium Artemisiae Argyi extractive liquid 10%, Dysosma versipellis carries
Take liquid 7%, garlic P.E 13%, Cosan 20%.
Heating-up temperature after the Honegsukle flower P.E, dandelion extract, Folium Artemisiae Argyi extract and the garlic P.E mixing that are taken
It is preferably with the time：55 DEG C, 18 hours.
Beneficial effect is：
1st, the present invention is used to prevent and treat the biological agent of tomato seedling damping-off, and its constituent honeysuckle, Dysosma versipellis and Cosan are in itself
With bactericidal action, with reference to the drive bacterium of Folium Artemisiae Argyi extractive liquid and its bactericidal effect, preferably sterilized and its prevention effect is reached,
Under the booster action of the dandelion extract in composition, it is optimal prevention effect；Garlic P.E can make as bleeding agent
Liquid rapid osmotic is in tomato plant；And the composition containing ginger in Folium Artemisiae Argyi extract, bactericidal effect is more preferably；The present invention is used for
The each component perfect adaptation of the biological agent of tomato seedling damping-off is prevented and treated, has preferably preventing and treating effect to the tomato seedling damping-off of tomato plant
Really.
2nd, the present invention is simple for preventing and treating the biological agent its preparation method of tomato seedling damping-off, with low cost, respectively constitutes into
Point innocuous substance is, in addition to Cosan, other components are extracted from plant, it is prepared for preventing and treating tomato seedling damping-off
Biological agent, green non-pollution to the nontoxic effect of people and animals, has control rate higher, reaches 90% to tomato seedling damping-off
More than, and drug effect is more long, up to 30 ~ 36 days.
Specific embodiment
A kind of preparation method of the biological agent for preventing and treating tomato seedling damping-off, including following preparation process：
The preparation of step one, Honegsukle flower P.E：Extracting honeysuckle 280g, removes impurity, after cleaning, drying, adds 1L water, heating
To after seething with excitement, slow fire boils truce in 30 minutes, and after filtering, gained supernatant is Honegsukle flower P.E, is cooled to room temperature
It is standby；
The preparation of step 2, dandelion extract：Dandelion complete stool 120g is taken, is cleaned, dried, crushed, be subsequently adding 1L
Petroleum ether immersion extracted within 48 hours, Buchner funnel suction filtration, collect filtrate；Petroleum ether is reclaimed in 55~65 DEG C of vacuum distillations,
Add the petroleum ether of recovery to soak in filter residue to be extracted, repeat extraction 4~5 times and reclaim filtrate；Suction filtration after repeatedly extracting
The filtrate decompression distillation and concentration for obtaining, obtains mixture, as dandelion extract, standby；
The preparation of step 3, Folium Artemisiae Argyi extract：Folium artemisiae argyi 150g is taken, impurity is removed, cleaned, add 1L water, be heated to 5 points after boiling
Clock, the ginger splices for adding 5g fresh, re-modulation slow fire boils truce in 30 minutes, and after filtering, gained supernatant is Folium Artemisiae Argyi extract,
Room temperature is cooled to, it is standby；
The preparation of step 4, Dysosma versipellis extract solution：500 grams of fresh Dysosma versipellis is taken to mash, after adding 1L water to soak 6 hours, filtering,
Gained supernatant is Dysosma versipellis extract solution, is placed in 4 DEG C of refrigerators and saves backup；
The preparation of step 5, garlic P.E：Choose fresh garlic, clean, dry after weigh 300g, be put in volumetric concentration
The min of surface sterilization 5 is carried out in 75% ethanol, with being placed in tissue refiner after aseptic water washing 3 times, add 100 mL without
Bacterium water, is homogenized 5 min and is made into pasty state, then 4 DEG C of temperature, rotating speed 10000r/ in centrifuge with the rotating speed of 5000r/min
10min is centrifuged under conditions of min, gained supernatant is to obtain garlic P.E, is placed in 4 DEG C of refrigerators and saves backup；
Step 6, it is 23-32% according to the mass percent of Honegsukle flower P.E, the mass percent of dandelion extract is 18-
25%, the mass percent of Folium Artemisiae Argyi extract is 8-15%, and the mass percent of Dysosma versipellis extract solution is 10-20%, garlic P.E
Mass percent be 8-15%, the mass percent of Cosan is 15-23%, takes the honeysuckle prepared by above-mentioned steps and extracts
Thing, dandelion extract, Folium Artemisiae Argyi extract, Dysosma versipellis extract solution, garlic P.E and the Cosan for preparing in addition, it is standby；
Heated after the mixing of step 7, the Honegsukle flower P.E that will be taken, dandelion extract, Folium Artemisiae Argyi extract and garlic P.E
17-19 hours is incubated to 45-60 DEG C, mixture I is obtained；Then taken Cosan is added to stir in mixture I, most
The biological agent for taking and preventing and treating tomato seedling damping-off being prepared after Dysosma versipellis extract solution is well mixed is added afterwards.
Institute extracting honeysuckle extract, dandelion extract, Folium Artemisiae Argyi extract, Dysosma versipellis extract solution, garlic P.E and sulphur
The mass percent of sulphur powder is preferably：Honegsukle flower P.E 25%, dandelion extract 25%, Folium Artemisiae Argyi extractive liquid 10%, Dysosma versipellis carries
Take liquid 7%, garlic P.E 13%, Cosan 20%.
Heating-up temperature after the Honegsukle flower P.E, dandelion extract, Folium Artemisiae Argyi extract and the garlic P.E mixing that are taken
It is preferably with the time：55 DEG C, 18 hours.
Step 8, every 50 plants of tomato plants, are 100ml using the biological agent of present invention preventing and treating tomato seedling damping-off, are added water
20-30L, fully shakes up, and complete stool is carried out to tomato plant and sprays mist to tear drop.
Embodiment 1
A kind of preparation method of the biological agent for preventing and treating tomato seedling damping-off, including following preparation process：
The preparation of step one, Honegsukle flower P.E：Extracting honeysuckle 280g, removes impurity, after cleaning, drying, adds 1L water, heating
To after seething with excitement, slow fire boils truce in 30 minutes, and after filtering, gained supernatant is Honegsukle flower P.E, is cooled to room temperature
It is standby；
The preparation of step 2, dandelion extract：Dandelion complete stool 120g is taken, is cleaned, dried, crushed, be subsequently adding 1L
Petroleum ether immersion extracted within 48 hours, Buchner funnel suction filtration, collect filtrate；Petroleum ether is reclaimed in 55~65 DEG C of vacuum distillations,
Add the petroleum ether of recovery to soak in filter residue to be extracted, repeat extraction 4~5 times and reclaim filtrate；Suction filtration after repeatedly extracting
The filtrate decompression distillation and concentration for obtaining, obtains mixture, as dandelion extract, standby；
The preparation of step 3, Folium Artemisiae Argyi extract：Folium artemisiae argyi 150g is taken, impurity is removed, cleaned, add 1L water, be heated to 5 points after boiling
Clock, the ginger splices for adding 5g fresh, re-modulation slow fire boils truce in 30 minutes, and after filtering, gained supernatant is Folium Artemisiae Argyi extract,
Room temperature is cooled to, it is standby；
The preparation of step 4, Dysosma versipellis extract solution：500 grams of fresh Dysosma versipellis is taken to mash, after adding 1L water to soak 6 hours, filtering,
Gained supernatant is Dysosma versipellis extract solution, is placed in 4 DEG C of refrigerators and saves backup；
The preparation of step 5, garlic P.E：Choose fresh garlic, clean, dry after weigh 300g, be put in volumetric concentration
The min of surface sterilization 5 is carried out in 75% ethanol, with being placed in tissue refiner after aseptic water washing 3 times, add 100 mL without
Bacterium water, is homogenized 5 min and is made into pasty state, then 4 DEG C of temperature, rotating speed 10000r/ in centrifuge with the rotating speed of 5000r/min
10min is centrifuged under conditions of min, gained supernatant is to obtain garlic P.E, is placed in 4 DEG C of refrigerators and saves backup；
Step 6, it is 25% according to the mass percent of Honegsukle flower P.E, the mass percent of dandelion extract is 25%, Chinese mugwort
The mass percent of leaf extract is 10%, and the mass percent of Dysosma versipellis extract solution is 10%, the quality percentage of garlic P.E
Than being 12%, the mass percent of Cosan is 18%, take Honegsukle flower P.E prepared by above-mentioned steps, dandelion extract,
Folium Artemisiae Argyi extract, Dysosma versipellis extract solution, garlic P.E and the Cosan for preparing in addition, it is standby；
Heated after the mixing of step 7, the Honegsukle flower P.E that will be taken, dandelion extract, Folium Artemisiae Argyi extract and garlic P.E
17-19 hours is incubated to 45-60 DEG C, mixture I is obtained；Then taken Cosan is added to stir in mixture I, most
The biological agent for taking and preventing and treating tomato seedling damping-off being prepared after Dysosma versipellis extract solution is well mixed is added afterwards.
Step 8, every 50 plants of tomato plants, are 100ml using the biological agent of present invention preventing and treating tomato seedling damping-off, are added water
20L, fully shakes up, and complete stool is carried out to tomato plant and sprays mist to tear drop.
Result shows：It is vertical to tomato after the biological agent of present invention preventing and treating tomato seedling damping-off sprays to tomato plant
The control rate of rot reaches 90%, and drug effect is up to 32 days.
Embodiment 2
A kind of preparation method of the biological agent for preventing and treating tomato seedling damping-off, including following preparation process：
The preparation of step one, Honegsukle flower P.E：Extracting honeysuckle 280g, removes impurity, after cleaning, drying, adds 1L water, heating
To after seething with excitement, slow fire boils truce in 30 minutes, and after filtering, gained supernatant is Honegsukle flower P.E, is cooled to room temperature
It is standby；
The preparation of step 2, dandelion extract：Dandelion complete stool 120g is taken, is cleaned, dried, crushed, be subsequently adding 1L
Petroleum ether immersion extracted within 48 hours, Buchner funnel suction filtration, collect filtrate；Petroleum ether is reclaimed in 55~65 DEG C of vacuum distillations,
Add the petroleum ether of recovery to soak in filter residue to be extracted, repeat extraction 4~5 times and reclaim filtrate；Suction filtration after repeatedly extracting
The filtrate decompression distillation and concentration for obtaining, obtains mixture, as dandelion extract, standby；
The preparation of step 3, Folium Artemisiae Argyi extract：Folium artemisiae argyi 150g is taken, impurity is removed, cleaned, add 1L water, be heated to 5 points after boiling
Clock, the ginger splices for adding 5g fresh, re-modulation slow fire boils truce in 30 minutes, and after filtering, gained supernatant is Folium Artemisiae Argyi extract,
Room temperature is cooled to, it is standby；
The preparation of step 4, Dysosma versipellis extract solution：500 grams of fresh Dysosma versipellis is taken to mash, after adding 1L water to soak 6 hours, filtering,
Gained supernatant is Dysosma versipellis extract solution, is placed in 4 DEG C of refrigerators and saves backup；
The preparation of step 5, garlic P.E：Choose fresh garlic, clean, dry after weigh 300g, be put in volumetric concentration
The min of surface sterilization 5 is carried out in 75% ethanol, with being placed in tissue refiner after aseptic water washing 3 times, add 100 mL without
Bacterium water, is homogenized 5 min and is made into pasty state, then 4 DEG C of temperature, rotating speed 10000r/ in centrifuge with the rotating speed of 5000r/min
10min is centrifuged under conditions of min, gained supernatant is to obtain garlic P.E, is placed in 4 DEG C of refrigerators and saves backup；
Step 6, it is 32% according to the mass percent of Honegsukle flower P.E, the mass percent of dandelion extract is 18%, Chinese mugwort
The mass percent of leaf extract is 15%, and the mass percent of Dysosma versipellis extract solution is 15%, the quality percentage of garlic P.E
Than being 15%, the mass percent of Cosan is 15%, take Honegsukle flower P.E prepared by above-mentioned steps, dandelion extract,
Folium Artemisiae Argyi extract, Dysosma versipellis extract solution, garlic P.E and the Cosan for preparing in addition, it is standby；
Heated after the mixing of step 7, the Honegsukle flower P.E that will be taken, dandelion extract, Folium Artemisiae Argyi extract and garlic P.E
17-19 hours is incubated to 45-60 DEG C, mixture I is obtained；Then taken Cosan is added to stir in mixture I, most
The biological agent for taking and preventing and treating tomato seedling damping-off being prepared after Dysosma versipellis extract solution is well mixed is added afterwards.
Step 8, every 50 plants of tomato plants, are 100ml using the biological agent of present invention preventing and treating tomato seedling damping-off, are added water
20-30L, fully shakes up, and complete stool is carried out to tomato plant and sprays mist to tear drop.
Result shows：It is vertical to tomato after the biological agent of present invention preventing and treating tomato seedling damping-off sprays to tomato plant
The control rate of rot reaches 92%, and drug effect is up to 36 days.
Embodiment 3
A kind of preparation method of the biological agent for preventing and treating tomato seedling damping-off, including following preparation process：
The preparation of step one, Honegsukle flower P.E：Extracting honeysuckle 280g, removes impurity, after cleaning, drying, adds 1L water, heating
To after seething with excitement, slow fire boils truce in 30 minutes, and after filtering, gained supernatant is Honegsukle flower P.E, is cooled to room temperature
It is standby；
The preparation of step 2, dandelion extract：Dandelion complete stool 120g is taken, is cleaned, dried, crushed, be subsequently adding 1L
Petroleum ether immersion extracted within 48 hours, Buchner funnel suction filtration, collect filtrate；Petroleum ether is reclaimed in 55~65 DEG C of vacuum distillations,
Add the petroleum ether of recovery to soak in filter residue to be extracted, repeat extraction 4~5 times and reclaim filtrate；Suction filtration after repeatedly extracting
The filtrate decompression distillation and concentration for obtaining, obtains mixture, as dandelion extract, standby；
The preparation of step 3, Folium Artemisiae Argyi extract：Folium artemisiae argyi 150g is taken, impurity is removed, cleaned, add 1L water, be heated to 5 points after boiling
Clock, the ginger splices for adding 5g fresh, re-modulation slow fire boils truce in 30 minutes, and after filtering, gained supernatant is Folium Artemisiae Argyi extract,
Room temperature is cooled to, it is standby；
The preparation of step 4, Dysosma versipellis extract solution：500 grams of fresh Dysosma versipellis is taken to mash, after adding 1L water to soak 6 hours, filtering,
Gained supernatant is Dysosma versipellis extract solution, is placed in 4 DEG C of refrigerators and saves backup；
The preparation of step 5, garlic P.E：Choose fresh garlic, clean, dry after weigh 300g, be put in volumetric concentration
The min of surface sterilization 5 is carried out in 75% ethanol, with being placed in tissue refiner after aseptic water washing 3 times, add 100 mL without
Bacterium water, is homogenized 5 min and is made into pasty state, then 4 DEG C of temperature, rotating speed 10000r/ in centrifuge with the rotating speed of 5000r/min
10min is centrifuged under conditions of min, gained supernatant is to obtain garlic P.E, is placed in 4 DEG C of refrigerators and saves backup；
Step 6, it is 23% according to the mass percent of Honegsukle flower P.E, the mass percent of dandelion extract is 18%, Chinese mugwort
The mass percent of leaf extract is 12%, and the mass percent of Dysosma versipellis extract solution is 17%, the quality percentage of garlic P.E
Than being 14%, the mass percent of Cosan is 16%, take Honegsukle flower P.E prepared by above-mentioned steps, dandelion extract,
Folium Artemisiae Argyi extract, Dysosma versipellis extract solution, garlic P.E and the Cosan for preparing in addition, it is standby；
Heated after the mixing of step 7, the Honegsukle flower P.E that will be taken, dandelion extract, Folium Artemisiae Argyi extract and garlic P.E
17-19 hours is incubated to 45-60 DEG C, mixture I is obtained；Then taken Cosan is added to stir in mixture I, most
The biological agent for taking and preventing and treating tomato seedling damping-off being prepared after Dysosma versipellis extract solution is well mixed is added afterwards.
Step 8, every 50 plants of tomato plants, are 100ml using the biological agent of present invention preventing and treating tomato seedling damping-off, are added water
20-30L, fully shakes up, and complete stool is carried out to tomato plant and sprays mist to tear drop.
Result shows：It is vertical to tomato after the biological agent of present invention preventing and treating tomato seedling damping-off sprays to tomato plant
The control rate of rot reaches 94%, and drug effect is up to 32 days.
Embodiment 4
A kind of preparation method of the biological agent for preventing and treating tomato seedling damping-off, including following preparation process：
The preparation of step one, Honegsukle flower P.E：Extracting honeysuckle 280g, removes impurity, after cleaning, drying, adds 1L water, heating
To after seething with excitement, slow fire boils truce in 30 minutes, and after filtering, gained supernatant is Honegsukle flower P.E, is cooled to room temperature
It is standby；
The preparation of step 2, dandelion extract：Dandelion complete stool 120g is taken, is cleaned, dried, crushed, be subsequently adding 1L
Petroleum ether immersion extracted within 48 hours, Buchner funnel suction filtration, collect filtrate；Petroleum ether is reclaimed in 55~65 DEG C of vacuum distillations,
Add the petroleum ether of recovery to soak in filter residue to be extracted, repeat extraction 4~5 times and reclaim filtrate；Suction filtration after repeatedly extracting
The filtrate decompression distillation and concentration for obtaining, obtains mixture, as dandelion extract, standby；
The preparation of step 3, Folium Artemisiae Argyi extract：Folium artemisiae argyi 150g is taken, impurity is removed, cleaned, add 1L water, be heated to 5 points after boiling
Clock, the ginger splices for adding 5g fresh, re-modulation slow fire boils truce in 30 minutes, and after filtering, gained supernatant is Folium Artemisiae Argyi extract,
Room temperature is cooled to, it is standby；
The preparation of step 4, Dysosma versipellis extract solution：500 grams of fresh Dysosma versipellis is taken to mash, after adding 1L water to soak 6 hours, filtering,
Gained supernatant is Dysosma versipellis extract solution, is placed in 4 DEG C of refrigerators and saves backup；
The preparation of step 5, garlic P.E：Choose fresh garlic, clean, dry after weigh 300g, be put in volumetric concentration
The min of surface sterilization 5 is carried out in 75% ethanol, with being placed in tissue refiner after aseptic water washing 3 times, add 100 mL without
Bacterium water, is homogenized 5 min and is made into pasty state, then 4 DEG C of temperature, rotating speed 10000r/ in centrifuge with the rotating speed of 5000r/min
10min is centrifuged under conditions of min, gained supernatant is to obtain garlic P.E, is placed in 4 DEG C of refrigerators and saves backup；
Step 6, it is 25% according to the mass percent of Honegsukle flower P.E, the mass percent of dandelion extract is 25%, Chinese mugwort
The mass percent of leaf extract is 10%, and the mass percent of Dysosma versipellis extract solution is 7%, the mass percent of garlic P.E
It is 13%, the mass percent of Cosan is 20%, takes Honegsukle flower P.E, dandelion extract, the Chinese mugwort prepared by above-mentioned steps
Leaf extract, Dysosma versipellis extract solution, garlic P.E and the Cosan for preparing in addition, it is standby；
Heated after the mixing of step 7, the Honegsukle flower P.E that will be taken, dandelion extract, Folium Artemisiae Argyi extract and garlic P.E
17-19 hours is incubated to 45-60 DEG C, mixture I is obtained；Then taken Cosan is added to stir in mixture I, most
The biological agent for taking and preventing and treating tomato seedling damping-off being prepared after Dysosma versipellis extract solution is well mixed is added afterwards.
Step 8, every 50 plants of tomato plants, are 100ml using the biological agent of present invention preventing and treating tomato seedling damping-off, are added water
20-30L, fully shakes up, and complete stool is carried out to tomato plant and sprays mist to tear drop.
Result shows：It is vertical to tomato after the biological agent of present invention preventing and treating tomato seedling damping-off sprays to tomato plant
The control rate of rot reaches 94%, and drug effect is up to 32 days.
Medication points for attention：The dusk of warm, wet weather or cloudy dispenser are preferably selected, and is forbidden same with bactericide, Basic pesticides
Phase or compounding use.The present invention for prevent and treat tomato seedling damping-off should not be long placed in place, should be now with the current, in order to avoid reduce drug effect.
Claims (3)
1. it is a kind of prevent and treat tomato seedling damping-off biological agent preparation method, it is characterised in that：Including following preparation process：
The preparation of step one, Honegsukle flower P.E：Extracting honeysuckle 280g, removes impurity, after cleaning, drying, adds 1L water, heating
To after seething with excitement, slow fire boils truce in 30 minutes, and after filtering, gained supernatant is Honegsukle flower P.E, is cooled to room temperature
It is standby；
The preparation of step 2, dandelion extract：Dandelion complete stool 120g is taken, is cleaned, dried, crushed, be subsequently adding 1L
Petroleum ether immersion extracted within 48 hours, Buchner funnel suction filtration, collect filtrate；Petroleum ether is reclaimed in 55~65 DEG C of vacuum distillations,
Add the petroleum ether of recovery to soak in filter residue to be extracted, repeat extraction 4~5 times and reclaim filtrate；Suction filtration after repeatedly extracting
The filtrate decompression distillation and concentration for obtaining, obtains mixture, as dandelion extract, standby；
The preparation of step 3, Folium Artemisiae Argyi extract：Folium artemisiae argyi 150g is taken, impurity is removed, cleaned, add 1L water, be heated to 5 points after boiling
Clock, the ginger splices for adding 5g fresh, re-modulation slow fire boils truce in 30 minutes, and after filtering, gained supernatant is Folium Artemisiae Argyi extract,
Room temperature is cooled to, it is standby；
The preparation of step 4, Dysosma versipellis extract solution：500 grams of fresh Dysosma versipellis is taken to mash, after adding 1L water to soak 6 hours, filtering,
Gained supernatant is Dysosma versipellis extract solution, is placed in 4 DEG C of refrigerators and saves backup；
The preparation of step 5, garlic P.E：Choose fresh garlic, clean, dry after weigh 300g, be put in volumetric concentration
The min of surface sterilization 5 is carried out in 75% ethanol, with being placed in tissue refiner after aseptic water washing 3 times, add 100 mL without
Bacterium water, is homogenized 5 min and is made into pasty state, then 4 DEG C of temperature, rotating speed 10000r/ in centrifuge with the rotating speed of 5000r/min
10min is centrifuged under conditions of min, gained supernatant is to obtain garlic P.E, is placed in 4 DEG C of refrigerators and saves backup；
Step 6, it is 23-32% according to the mass percent of Honegsukle flower P.E, the mass percent of dandelion extract is 18-
25%, the mass percent of Folium Artemisiae Argyi extract is 8-15%, and the mass percent of Dysosma versipellis extract solution is 10-20%, garlic P.E
Mass percent be 8-15%, the mass percent of Cosan is 15-23%, takes the honeysuckle prepared by above-mentioned steps and extracts
Thing, dandelion extract, Folium Artemisiae Argyi extract, Dysosma versipellis extract solution, garlic P.E and the Cosan for preparing in addition, it is standby；
Heated after the mixing of step 7, the Honegsukle flower P.E that will be taken, dandelion extract, Folium Artemisiae Argyi extract and garlic P.E
17-19 hours is incubated to 45-60 DEG C, mixture I is obtained；Then taken Cosan is added to stir in mixture I, most
The biological agent for taking and preventing and treating tomato seedling damping-off being prepared after Dysosma versipellis extract solution is well mixed is added afterwards.
2. the preparation method of the biological agent of tomato seedling damping-off is prevented and treated as claimed in claim 1, it is characterised in that：Institute's extracting honeysuckle
The mass percent of extract, dandelion extract, Folium Artemisiae Argyi extract, Dysosma versipellis extract solution, garlic P.E and Cosan is：
Honegsukle flower P.E 25%, dandelion extract 25%, Folium Artemisiae Argyi extractive liquid 10%, Dysosma versipellis extract solution 7%, garlic P.E 13%, sulphur
Sulphur powder 20%.
3. the biological agent of tomato seedling damping-off is prevented and treated as claimed in claim 1 or 2, it is characterised in that：The honeysuckle for being taken is extracted
55 DEG C are heated to after the mixing of thing, dandelion extract, Folium Artemisiae Argyi extract and garlic P.E be incubated 18 hours.
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