CN105612857A - Pretreatment method for promoting radix angelicae seed germination - Google Patents
Pretreatment method for promoting radix angelicae seed germination Download PDFInfo
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- CN105612857A CN105612857A CN201510951077.1A CN201510951077A CN105612857A CN 105612857 A CN105612857 A CN 105612857A CN 201510951077 A CN201510951077 A CN 201510951077A CN 105612857 A CN105612857 A CN 105612857A
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- radix angelicae
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- angelicae dahuricae
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/02—Germinating apparatus; Determining germination capacity of seeds or the like
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Abstract
The invention discloses a pretreatment method for promoting radix angelicae seed germination. The pretreatment method comprises the specific steps of physical membrane removal, low-temperature refrigeration, high-temperature ethanol water solution soaking, low-temperature potassium nitrate water solution infiltration, ionizing radiation and sowing. According to the pretreatment method for promoting radix angelicae seed germination, various methods are combined to play a synergistic effect, and the time for pretreatment, which is more than 10 hours, is shortened to be 3-4 hours, so that the work efficiency is improved, and the germination rate of radix angelicae seeds is increased to be 96-98%;wing membranes are removed according to a physical method, so that moisture and reagent access infiltration is facilitated, and the aftertreatment time is shortened; the seeds are placed in the low-temperature and high-temperature environments, seed germination is promoted through temperature difference adverse situation, and meanwhile the stress resistance of the seeds and seedlings is improved; the seeds are infiltrated with a high-temperature ethanol water solution and a low-temperature potassium nitrate water solution, so that hard coats of the seeds are corroded and damaged, and meanwhile the seeds are sterilized; through radiation treatment, a growing enzyme in the seeds can be activated, seed germination can be induced, and the yield and quality of radix angelicae can be improved.
Description
Technical field
The present invention relates to seedling propagation field, specifically, relate to a kind of pre-treating method promoting Radix Angelicae Dahuricae seed germination.
Background technology
The Radix Angelicae Dahuricae, another name Qi Baizhi, angelica anomala, Radix angelicae dahuricae, Radix Angelicae Dahuricae (Radix Heraclei Scabridi) etc., the plant of Umbelliferae angelica. It is used as medicine with root, the dehumidifying that gets rid of illness, wind-expelling pain-stopping, evacuation of pus granulation promoting, promoting blood circulation and stopping pain, the function such as anti-inflammation, cure mainly anemofrigid cold, headache, rhinitis, toothache, leucorrhea with red and white discharge, the disease such as pain furuncle and phyma poison, also can make spice.
The Radix Angelicae Dahuricae is distributed in the ground such as northeast and the North China of China's Mainland, is grown on the area of height above sea level 200 meters to 1500 meters. Weather conditions being required not strict, integrated distribution moistens at warmer climate, sunny, and rainfall is abundant, loosing soil, area deep, fertile, well-drained. But, in existing Radix Angelicae Dahuricae cultivation technique, Radix Angelicae Dahuricae seed is commonly performed conventional treatment, and germination percentage is low, affects yield and the quality of the later stage Radix Angelicae Dahuricae, and seed processing technology needs to optimize further and improve.
Summary of the invention
In order to make up the defect of prior art, it is an object of the invention to provide a kind of pre-treating method promoting Radix Angelicae Dahuricae seed germination.
A kind of pre-treating method promoting Radix Angelicae Dahuricae seed germination, concrete comprises the following steps:
(1) select full, ripe, complete newly to receive Radix Angelicae Dahuricae seed then, remove parachute, obtain striping seed;
(2) striping seed is placed in the refrigerator of 2 ~ 4 DEG C, after freezing 90 ~ 100min, takes out, obtain freezing seed;
(3) putting at once after being taken out by freezing seed in the ethanol water of volume fraction 63 ~ 65% (v/v), ethanol water temperature is 40 ~ 45 DEG C, soaks 3 ~ 5min, takes out, is rinsed well by ethanol water with clear water, dewatering, obtain ethanol seed;
(4) ethanol seed is placed in the environment of 4 ~ 6 DEG C, ethanol seed is uniformly sprayed with the Alkitrate that mass fraction is 1.2 ~ 1.5% (w/w), infiltration 10 ~ 15min, improve ambient temperature to 16 ~ 18 DEG C, infiltration 15 ~ 20min, continue to improve ambient temperature to 25 ~ 27 DEG C, infiltrate 25 ~ 30min, obtain potassium nitrate seed;
(5) potassium nitrate seed being carried out ionizing radiation, intensity is 60 ~ 80Gy, radiates 6 ~ 8min, takes out, obtains radiation seed;
(6) sow.
The ionizing radiation of described step (5), for radiation 2min, stopping 1min, only radiated time is 6 ~ 8min.
The invention have the advantage that a kind of pre-treating method promoting Radix Angelicae Dahuricae seed germination provided by the invention, multiple method combines, synergism, by Radix Angelicae Dahuricae seed pre-treatment tens hours, shorten to 3 ~ 4 hours, save the time, improve work efficiency, significantly improve Radix Angelicae Dahuricae seed germination rate, reach 96 ~ 98%; Physical removes parachute, is conducive to the entrance of moisture and reagent, shortens the process time in later stage; It is placed in low temperature and hot environment by staggered for seed, promotes seed germination by temperature difference adverse circumstance, the resistance of seed and seedling can be increased simultaneously; Infiltrate seed by high temperature ethanol water and low temperature Alkitrate, it is possible to the hard epidermis of corrosion and destruction seed, seed can be sterilized simultaneously, reduce disease pest rate; In low temperature Alkitrate impregnation process, gradually step up ambient temperature, improve the seed adaptability to sprouting environment; Radiation treatment can grow enzyme in activated seed, brings out seed germination, strengthens vitality and resistance, improves yield and the quality of the Radix Angelicae Dahuricae.
Detailed description of the invention
With specific embodiment, the present invention is described below, but is not limitation of the present invention.
Embodiment 1
A kind of pre-treating method promoting Radix Angelicae Dahuricae seed germination, concrete comprises the following steps:
(1) select full, ripe, complete newly to receive Radix Angelicae Dahuricae seed then, remove parachute, obtain striping seed;
(2) striping seed is placed in the refrigerator of 2 DEG C, after freezing 90min, takes out, obtain freezing seed;
(3) putting at once after being taken out by freezing seed in the ethanol water of volume fraction 63% (v/v), ethanol water temperature is 40 DEG C, soaks 3min, takes out, is rinsed well by ethanol water with clear water, dewatering, obtain ethanol seed;
(4) ethanol seed is placed in the environment of 4 DEG C, uniformly sprays ethanol seed with the Alkitrate that mass fraction is 1.2% (w/w), infiltrate 10min, improve ambient temperature to 16 DEG C, infiltrate 15min, continue to improve ambient temperature to 25 DEG C, infiltration 25min, obtains potassium nitrate seed;
(5) potassium nitrate seed being carried out ionizing radiation, intensity is 60Gy, radiates 2min, stops 1min, repeats 3 times, radiates 6min, takes out, obtains radiation seed;
(6) sow.
Embodiment 2
A kind of pre-treating method promoting Radix Angelicae Dahuricae seed germination, concrete comprises the following steps:
(1) select full, ripe, complete newly to receive Radix Angelicae Dahuricae seed then, remove parachute, obtain striping seed;
(2) striping seed is placed in the refrigerator of 3 DEG C, after freezing 95min, takes out, obtain freezing seed;
(3) putting at once after being taken out by freezing seed in the ethanol water of volume fraction 64% (v/v), ethanol water temperature is 43 DEG C, soaks 4min, takes out, is rinsed well by ethanol water with clear water, dewatering, obtain ethanol seed;
(4) ethanol seed is placed in the environment of 5 DEG C, uniformly sprays ethanol seed with the Alkitrate that mass fraction is 1.3% (w/w), infiltrate 13min, improve ambient temperature to 17 DEG C, infiltrate 18min, continue to improve ambient temperature to 26 DEG C, infiltration 28min, obtains potassium nitrate seed;
(5) potassium nitrate seed being carried out ionizing radiation, intensity is 60 ~ 80Gy, radiates 2min, stops 1min, repeats 3 times, radiates 6min, takes out, obtains radiation seed;
(6) sow.
Embodiment 3
A kind of pre-treating method promoting Radix Angelicae Dahuricae seed germination, concrete comprises the following steps:
(1) select full, ripe, complete newly to receive Radix Angelicae Dahuricae seed then, remove parachute, obtain striping seed;
(2) striping seed is placed in the refrigerator of 4 DEG C, after freezing 100min, takes out, obtain freezing seed;
(3) putting at once after being taken out by freezing seed in the ethanol water of volume fraction 65% (v/v), ethanol water temperature is 45 DEG C, soaks 5min, takes out, is rinsed well by ethanol water with clear water, dewatering, obtain ethanol seed;
(4) ethanol seed is placed in the environment of 6 DEG C, uniformly sprays ethanol seed with the Alkitrate that mass fraction is 1.5% (w/w), infiltrate 15min, improve ambient temperature to 18 DEG C, infiltrate 20min, continue to improve ambient temperature to 27 DEG C, infiltration 30min, obtains potassium nitrate seed;
(5) potassium nitrate seed being carried out ionizing radiation, intensity is 60 ~ 80Gy, radiates 2min, stops 1min, repeats 4 times, radiates 8min, takes out, obtains radiation seed;
(6) sow.
Comparative example 1
A kind of pre-treating method promoting Radix Angelicae Dahuricae seed germination, concrete comprises the following steps:
(1) select full, ripe, complete newly to receive Radix Angelicae Dahuricae seed then, remove parachute, obtain striping seed;
(2) being put into by striping seed in the ethanol water of volume fraction 60% (v/v), ethanol water temperature is 45 DEG C, soaks 5min, takes out, is rinsed well by ethanol water with clear water, dewatering, obtain ethanol seed;
(3) ethanol seed is placed in the environment of 6 DEG C, uniformly sprays ethanol seed with the Alkitrate that mass fraction is 1.5% (w/w), infiltrate 65min, obtain potassium nitrate seed;
(4) potassium nitrate seed being carried out ionizing radiation, intensity is 60Gy, radiates 8min, takes out, obtains radiation seed;
(5) sow.
Comparative example 2
A kind of pre-treating method promoting Radix Angelicae Dahuricae seed germination, concrete comprises the following steps:
(1) select full, ripe, complete newly to receive Radix Angelicae Dahuricae seed then, remove parachute, obtain striping seed;
(2) striping seed is placed in the refrigerator of 3 DEG C, after freezing 95min, takes out, obtain freezing seed;
(3) freezing seed is placed in the environment of 5 DEG C, uniformly sprays ethanol seed with the Alkitrate that mass fraction is 1.0% (w/w), infiltrate 60min, obtain potassium nitrate seed;
(4) potassium nitrate seed being carried out ionizing radiation, intensity is 60Gy, radiates 6min, takes out, obtains radiation seed;
(5) sow.
Comparative example 3
A kind of pre-treating method promoting Radix Angelicae Dahuricae seed germination, concrete comprises the following steps:
(1) select full, ripe, complete newly to receive Radix Angelicae Dahuricae seed then, remove parachute, obtain striping seed;
(2) striping seed is placed in the refrigerator of 4 DEG C, after freezing 50min, takes out, obtain freezing seed;
(3) putting at once after being taken out by freezing seed in the ethanol water of volume fraction 56% (v/v), ethanol water temperature is 24 DEG C, soaks 5min, takes out, is rinsed well by ethanol water with clear water, dewatering, obtain ethanol seed;
(4) ethanol seed is carried out ionizing radiation, intensity 70Gy, radiate 8min, obtain radiation seed;
(5) sow.
Comparative example 4
A kind of pre-treating method promoting Radix Angelicae Dahuricae seed germination, concrete comprises the following steps:
(1) select full, ripe, complete newly to receive Radix Angelicae Dahuricae seed then, remove parachute, obtain striping seed;
(2) striping seed is placed in the refrigerator of 4 DEG C, after freezing 100min, takes out, obtain freezing seed;
(3) putting at once after being taken out by freezing seed in the ethanol water of volume fraction 60% (v/v), ethanol water temperature is 45 DEG C, soaks 5min, takes out, is rinsed well by ethanol water with clear water, dewatering, obtain ethanol seed;
(4) ethanol seed is placed in the environment of 6 DEG C, uniformly sprays ethanol seed with the Alkitrate that mass fraction is 1.5% (w/w), infiltrate 55min, obtain potassium nitrate seed;
(5) sow.
The sprouting of embodiment and comparative example Radix Angelicae Dahuricae seed and upgrowth situation:
In areal, according to the distinct methods of embodiment and comparative example, Radix Angelicae Dahuricae seed is carried out pre-treatment, plant and manage identical, observe and calculate the upgrowth situation of the Radix Angelicae Dahuricae. The sprouting of embodiment and comparative example Radix Angelicae Dahuricae seed and upgrowth situation are in Table 1.
Table 1: the sprouting of embodiment and comparative example Radix Angelicae Dahuricae seed and upgrowth situation
| Project | Germination percentage/(%) | Disease pest rate/(%) | Excellent rate/(%) | Yield (does)/(Kg/ mu) |
| Embodiment 1 | 96 | 5 | 58.5 | 605.6 |
| Embodiment 2 | 97 | 4 | 62.7 | 624.4 |
| Embodiment 3 | 98 | 3 | 67.4 | 635.1 |
| Comparative example 1 | 67 | 12 | 37.6 | 573.5 |
| Comparative example 2 | 59 | 14 | 34.4 | 554.7 |
| Comparative example 3 | 57 | 15 | 32.5 | 546.4 |
| Comparative example 4 | 54 | 18 | 29.6 | 523.3 |
As can be seen from Table 1, the Radix Angelicae Dahuricae seed that the pre-treating method promoting Radix Angelicae Dahuricae seed germination of embodiment obtains, substantially relatively comparative example is high for germination rate, substantially relatively comparative example is low for the disease pest rate of Radix Angelicae Dahuricae plant, substantially relatively comparative example is high for excellent rate and yield, illustrating that the pre-treating method of promotion Radix Angelicae Dahuricae seed germination provided by the invention can effectively facilitate Radix Angelicae Dahuricae seed germination, improve resistance, yield and quality are all significantly improved.
The nutrient of embodiment and the comparative example Radix Angelicae Dahuricae:
In areal, according to the distinct methods of embodiment and comparative example, Radix Angelicae Dahuricae seed is processed, carry out identical plantation and management, after gathering, each Radix Angelicae Dahuricae is carried out the mensuration of nutritive element content. The nutrient of embodiment and the comparative example Radix Angelicae Dahuricae is in Table 2.
Table 2: the nutrient of embodiment and the comparative example Radix Angelicae Dahuricae
| Project | Selenium/(mg/Kg) | Zinc/(mg/Kg) | Ferrum/(mg/Kg) | Imperatorin/(g/Kg) |
| Embodiment 1 | 0.56 | 9.45 | 56.82 | 3.36 |
| Embodiment 2 | 0.62 | 9.86 | 61.47 | 3.72 |
| Embodiment 3 | 0.76 | 10.33 | 67.62 | 4.26 |
| Comparative example 1 | 0.46 | 8.66 | 49.56 | 2.35 |
| Comparative example 2 | 0.43 | 8.53 | 47.32 | 1.98 |
| Comparative example 3 | 0.41 | 8.45 | 46.46 | 1.93 |
| Comparative example 4 | 0.34 | 8.26 | 44.56 | 1.75 |
As can be seen from Table 2, substantially relatively comparative example is high for the selenium of the Radix Angelicae Dahuricae, zinc, ferrum and the imperatorin content that the pre-treating method promoting Radix Angelicae Dahuricae seed germination of embodiment obtains, illustrating that the Radix Angelicae Dahuricae nutrient that the Radix Angelicae Dahuricae seed pre-treatment processing method of the present invention obtains is high, health care is good.
Claims (2)
1. the pre-treating method promoting Radix Angelicae Dahuricae seed germination, it is characterised in that specifically include following steps:
(1) select full, ripe, complete newly to receive Radix Angelicae Dahuricae seed then, remove parachute, obtain striping seed;
(2) striping seed is placed in the refrigerator of 2 ~ 4 DEG C, after freezing 90 ~ 100min, takes out, obtain freezing seed;
(3) putting at once after being taken out by freezing seed in the ethanol water of volume fraction 63 ~ 65% (v/v), ethanol water temperature is 40 ~ 45 DEG C, soaks 3 ~ 5min, takes out, is rinsed well by ethanol water with clear water, dewatering, obtain ethanol seed;
(4) ethanol seed is placed in the environment of 4 ~ 6 DEG C, ethanol seed is uniformly sprayed with the Alkitrate that mass fraction is 1.2 ~ 1.5% (w/w), infiltration 10 ~ 15min, improve ambient temperature to 16 ~ 18 DEG C, infiltration 15 ~ 20min, continue to improve ambient temperature to 25 ~ 27 DEG C, infiltrate 25 ~ 30min, obtain potassium nitrate seed;
(5) potassium nitrate seed being carried out ionizing radiation, intensity is 60 ~ 80Gy, radiates 6 ~ 8min, takes out, obtains radiation seed;
(6) sow.
2. promoting the pre-treating method of Radix Angelicae Dahuricae seed germination according to claim 1, it is characterised in that the ionizing radiation of described step (5), for radiation 2min, stopping 1min, only radiated time is 6 ~ 8min.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108521932A (en) * | 2018-05-14 | 2018-09-14 | 甘肃省农业科学院经济作物与啤酒原料研究所（甘肃省农业科学院中药材研究所） | A method of promoting Radix Angelicae Sinensis breeding base material germination |
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2015
- 2015-12-19 CN CN201510951077.1A patent/CN105612857A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108521932A (en) * | 2018-05-14 | 2018-09-14 | 甘肃省农业科学院经济作物与啤酒原料研究所（甘肃省农业科学院中药材研究所） | A method of promoting Radix Angelicae Sinensis breeding base material germination |
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