CN105580641B - It is a kind of using Eucalyptus urophylla-grandis as the method for planting almond abalone mushroom of raw material - Google Patents

It is a kind of using Eucalyptus urophylla-grandis as the method for planting almond abalone mushroom of raw material Download PDF

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CN105580641B
CN105580641B CN201610041440.0A CN201610041440A CN105580641B CN 105580641 B CN105580641 B CN 105580641B CN 201610041440 A CN201610041440 A CN 201610041440A CN 105580641 B CN105580641 B CN 105580641B
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bacteria
grandis
mushroom
temperature
raw material
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CN105580641A (en
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杨艺辉
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FUJIAN JIATIAN AGRICULTURAL DEVELOPMENT Co Ltd
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FUJIAN JIATIAN AGRICULTURAL DEVELOPMENT Co Ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F1/00Fertilisers made from animal corpses, or parts thereof
    • C05F1/005Fertilisers made from animal corpses, or parts thereof from meat-wastes or from other wastes of animal origin, e.g. skins, hair, hoofs, feathers, blood

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  • Life Sciences & Earth Sciences (AREA)
  • Mycology (AREA)
  • Environmental Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Environmental & Geological Engineering (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Mushroom Cultivation (AREA)

Abstract

The invention discloses a kind of using Eucalyptus urophylla-grandis as the method for planting almond abalone mushroom of raw material, includes the following steps:1)Dispensing;2)Pack;3)Sterilizing;4)It is cooling;5)Inoculation;6)Bacteria;7)Flower bud;8)Management of producing mushroom;9)Harvesting, wherein step 2)Pack can use bottling replace, but need in step 6)Mycelium stimulation is carried out later.The present invention cultivates Pleurotus eryngii using Eucalyptus urophylla-grandis sawdust as primary raw material, cultivate obtained Pleurotus eryngii good quality, mouthfeel is crisp and refreshing, reduce cost of material, cultivation period is shortened, enterprise's production cost has been saved, and increases the utilization to fast-growing woods Eucalyptus urophylla-grandis, the contradiction with Lin Zhengmu is alleviated, it is both economically and environmentally beneficial.

Description

It is a kind of using Eucalyptus urophylla-grandis as the method for planting almond abalone mushroom of raw material
Technical field
The invention belongs to fungus growing technique fields, and in particular to a kind of using Eucalyptus urophylla-grandis as the planting almond abalone mushroom side of raw material Method.
Background technology
China's afforestation rate is not high, its demand to the forest reserves is cultivated on a large scale increasingly with edible mushroom in recent years It increases, the influence to natural environment is also further apparent.Although the artificial fast-growing woods in China quickly grows in recent years, the greening to environment Effect is also more significant, but insufficient for the utilization of artificial fast-growing woods, causes a large amount of waste.
The nutrition very abundant of Pleurotus eryngii, plant protein content are up to 25%, containing 18 kinds of amino acid needed by human and With improve the immunity of the human body, the polysaccharide of cancer-resisting.Meanwhile it contains a large amount of oligosaccharides, is 15 times, needle mushroom of grifola frondosus 3.5 times, 2 times of true pleurotus cornucopiae, it works with the bifid bacterium one in stomach and intestine, have promote to digest well, absorption function.Apricot Abalone mushroom is that a kind of mouthfeel is very good and Rare edible fungus new varieties with medicinal function, deep to be welcome by domestic and international market.But For the culturing raw material of Pleurotus eryngii based on weed tree sawdust, cotton seed hulls, corncob, growth cycle is long at present, and cost of material is also higher.
Invention content
It is an object of the invention to:There is provided it is a kind of using Eucalyptus urophylla-grandis as the method for planting almond abalone mushroom of raw material, use Eucalyptus urophylla-grandis wood Bits cultivation Pleurotus eryngii, not only cost of material is relatively low but also since Eucalyptus urophylla-grandis sawdust is more loose, and aeration is good, and water retention property is good, Pleurotus eryngii cultivation cycle can be shortened, reduce production cost.
To achieve the above object, the present invention uses following technical scheme:
It is a kind of using Eucalyptus urophylla-grandis as the method for planting almond abalone mushroom of raw material in the present invention, include the following steps:
1)By following mass fraction dispensing is carried out than formula:Eucalyptus urophylla-grandis sawdust 64%, wheat bran 15%, corn flour 10%, dregs of beans 10%, oyster shell powder 1%;
2)Pack:It is packed into bacterium bag after adding water to stir evenly by above-mentioned formula, and water content is made to be 63%~65%;
3)Sterilizing:Using high pressure sterilization or normal-pressure sterilization, the pH range that sterilized is 6-6.4, wherein high pressure sterilization item Part is that culture medium maintains 121 DEG C, 2.5 hours or more;Normal-pressure sterilization condition is that culture medium maintains 100 DEG C, 12h or more;
4)It is cooling:It is rapidly cooled to bag under bacterium bag is moved on to gnotobasis in 80 DEG C of culture medium temperature or more after sterilizing 25 DEG C of interior temperature or less;
5)Inoculation:It is inoculated in special aseptic inoculation room after the completion of cooling, and periodically environment is cleaned and disappeared Poison, it is ensured that inoculation environment is dustless, sterile, and the strain after inoculation is made to cover entire charge level;
6)Bacteria:After inoculation, bacteria room is moved in time and is cultivated, bacteria room will be cleaned, be sterilized in advance, and be ensured Air quality i.e. air up to standard falls bacterium 30 minutes 5 or less during entire bacteria;
7)Flower bud:Flower bud is carried out after mycelia is fully ripe, between 10~18 DEG C, space is wet for temperature control during flower bud Degree maintains 90%~95%, increases scattering light stimulus, reinforces ventilation, and reduces CO inside mushroom house2Concentration, by 3~6d, i.e., Former base can be formed, former base is further differentiated to form mushroom flower bud;
8)Management of producing mushroom:When former base is differentiated to form 1~2 cm small mushroom buds, bag is opened in due course, and temperature should be maintained at 10~18 Within the scope of DEG C, and temperature is higher in early period, and later stage bacteria cover diameter is grown to 2~3cm, reduces temperature;Relative air humidity is kept 89%~91%, the later stage can suitably reduce humidity;Intensity of illumination is advisable with 500~1 000lx, and the later stage can suitably increase illumination;
9)Harvesting:When cap is open and flat, spore not yet launches, bacteria cover diameter it is consistent with stem or slightly smaller than stem when it is necessary to Harvesting in time.
Preferably, the step 1)In formula can also be Eucalyptus urophylla-grandis sawdust 60%, corncob 17%, wheat bran 15%, corn Powder 7%, dregs of beans 10%, oyster shell powder 1%.
Preferably, the step 2)Middle bacterium bag specification is 17*35, and wet feed weighs 1100~1200 grams in bag.
Preferably, the step 2)Can also be to be bottled using bacterium bottle, and bacterium bottle specification is 1100 milliliters, it is wet in bottle Material weighs 650~680 grams.
Preferably, the step 6)Mycelium stimulation is carried out after the completion of middle bacteria:The old strain in bottleneck surface layer is scraped off with mushroom culturing device, and is made Bottleneck charge level flattens, and bottle, which stands upside down, after mycelium stimulation places, and mycelia is allowed to restore.
Preferably, in step 8)It is middle by inverted bacterium bottle upright, temperature, relative air humidity and intensity of illumination and bacterium bag phase Together.
Preferably, the step 6)Three-stage culture can be used when middle bacteria:Initial stage room temperature is advisable with 22 DEG C~24 DEG C, bacterium After material temperature in bag rises, room temperature should be turned down to 18 DEG C~22 DEG C, and mycelia covers with rear room temperature and can suitably be turned up, entire bacteria rank Section, material temperature all do not exceed 28 DEG C, and in the bacteria stage, and culturing room remains dark, relative air humidity control 70% with It is interior, and frequent ventilation.
The beneficial effects of the present invention are the present invention cultivates Pleurotus eryngii using Eucalyptus urophylla-grandis sawdust as primary raw material, cultivates The Pleurotus eryngii good quality arrived, mouthfeel is crisp and refreshing, reduces cost of material, shortens cultivation period, has saved enterprise's production cost, The utilization to fast-growing woods Eucalyptus urophylla-grandis is increased again, alleviates the contradiction with Lin Zhengmu, it is both economically and environmentally beneficial.
Specific implementation mode
The present invention is further described below with reference to specific embodiment.
Embodiment 1
It is a kind of using Eucalyptus urophylla-grandis as the method for planting almond abalone mushroom of raw material, include the following steps:
1)By following mass fraction dispensing is carried out than formula:Eucalyptus urophylla-grandis sawdust 64%, wheat bran 15%, corn flour 10%, dregs of beans 10%, oyster shell powder 1%;
2)Pack:It is packed into bacterium bag after adding water to stir evenly by above-mentioned formula, bacterium bag specification is 17*35, and wet feed weight in bag 1100~1200 grams, and water content is made to be 63%~65%;
3)Sterilizing:Using high pressure sterilization or normal-pressure sterilization, the pH range that sterilized is 6-6.4, wherein high pressure sterilization item Part is that culture medium maintains 121 DEG C, 2.5h or more;Normal-pressure sterilization condition is that culture medium maintains 100 DEG C, 12h or more;
4)It is cooling:It is rapidly cooled in bag under bacterium bag is moved on to gnotobasis in 80 DEG C of culture medium temperature or more after sterilizing 25 DEG C of temperature or less;
5)Inoculation:It is inoculated in special aseptic inoculation room after the completion of cooling, and periodically environment is cleaned and disappeared Poison, it is ensured that inoculation environment is dustless, sterile, and the strain after inoculation is made to cover entire charge level;
6)Bacteria:After inoculation, bacteria room is moved in time and is cultivated, bacteria room will be cleaned, be sterilized in advance, and be ensured Air quality i.e. air up to standard falls bacterium 30 minutes 5 or less during entire bacteria;Three-stage culture is used when bacteria:Initial stage room Temperature is advisable with 22 DEG C~24 DEG C, and after the material temperature in bacterium bag rises, room temperature should be turned down to 18 DEG C~22 DEG C, and mycelia covers with rear room temperature can Appropriate to be turned up, entire bacteria stage, material temperature does not all exceed 28 DEG C, and in the bacteria stage, and culturing room remains dark, air phase To humid control within 70%, and frequent ventilation;
7)Flower bud:Flower bud is carried out after mycelia is fully ripe, between 10~18 DEG C, space is wet for temperature control during flower bud Degree maintains 90%~95%, increases scattering light stimulus, reinforces ventilation, and reduces CO inside mushroom house2Concentration, by 3~6d, i.e., Former base can be formed, former base is further differentiated to form mushroom flower bud;
8)Management of producing mushroom:When former base is differentiated to form 1~2cm small mushroom buds, bag is opened in due course, and temperature should be maintained at 10~18 DEG C In range, and temperature is higher in early period, and later stage bacteria cover diameter is grown to 2~3cm, reduces temperature;Relative air humidity is maintained at 89%~91%, the later stage can suitably reduce humidity;Intensity of illumination is advisable with 500~1 000lx, and the later stage can suitably increase illumination;
9)Harvesting:When cap is open and flat, spore not yet launches, bacteria cover diameter it is consistent with stem or slightly smaller than stem when, in time Harvesting.
Embodiment 2
With above-described embodiment 1 the difference is that, in the present embodiment, step 2)It is middle to replace bacterium bag and bacterium bottle is used to carry out Bottling, and bacterium bottle specification is 1100 milliliters, wet feed weighs 650~680 grams in bottle;In step 6)Mycelium stimulation is carried out after the completion of middle bacteria: The old strain in bottleneck surface layer is scraped off with mushroom culturing device, and bottleneck charge level is made to flatten, bottle, which stands upside down, after mycelium stimulation places, and mycelia is allowed to restore;And In step 8)It is middle that inverted bacterium bottle is upright, it is identical when temperature, relative air humidity and intensity of illumination condition are with using bacterium bag.
Embodiment 3
With above-described embodiment 1 the difference is that, in the present embodiment, step 1)In formula be Eucalyptus urophylla-grandis sawdust 60%, Corncob 17%, wheat bran 15%, corn flour 7%, dregs of beans 10%, oyster shell powder 1%.
Embodiment 4
With above-described embodiment 2 the difference is that, in the present embodiment, step 1)Middle formula is Eucalyptus urophylla-grandis sawdust 60%, Corncob 17%, wheat bran 15%, corn flour 7%, dregs of beans 10%, oyster shell powder 1%.
As known by the technical knowledge, the present invention can pass through the embodiment party of other essence without departing from its spirit or essential feature Case is realized.Therefore, embodiment disclosed above, all things considered are all merely illustrative, not the only.Institute Have within the scope of the present invention or is including by the present invention equal to the change in the scope of the present invention.

Claims (5)

1. a kind of using Eucalyptus urophylla-grandis as the method for planting almond abalone mushroom of raw material, which is characterized in that include the following steps:
1) it presses following mass fraction and carries out dispensing than formula:Eucalyptus urophylla-grandis sawdust 64%, wheat bran 15%, corn flour 10%, dregs of beans 10%, oyster shell powder 1%;
2) it packs:It is packed into bacterium bag after adding water to stir evenly by above-mentioned formula, and water content is made to be 63%~65%;
3) it sterilizes:Using high pressure sterilization or normal-pressure sterilization, the pH range that sterilized is 6-6.4, wherein autoclave conditions are Culture medium maintains 121 DEG C, 2.5h or more;Normal-pressure sterilization condition is that culture medium maintains 100 DEG C, 12h or more;
4) cooling:It is rapidly cooled to temperature in bag under bacterium bag is moved on to gnotobasis in 80 DEG C of culture medium temperature or more after sterilizing 25 DEG C or less;
5) it is inoculated with:It is inoculated in special aseptic inoculation room after the completion of cooling, and periodically environment is cleaned and sterilized, really It is dustless, sterile to protect inoculation environment, the strain after inoculation is made to cover entire charge level;
6) bacteria:After inoculation, bacteria room being moved in time and is cultivated, bacteria room will be cleaned, be sterilized in advance, and be ensured entire Air quality i.e. air up to standard falls bacterium 30 minutes 5 or less during bacteria;
7) flower bud:Flower bud is carried out after mycelia is fully ripe, temperature control is between 10~18 DEG C during flower bud, space humidity dimension It holds 90%~95%, increases scattering light stimulus, reinforce ventilation, and reduce CO inside mushroom house2Concentration, by 3~6d, you can shape At former base, former base is further differentiated to form mushroom flower bud;
8) management of producing mushroom:When former base is differentiated to form 1~2cm small mushroom buds, bag is opened in due course, and temperature should be maintained at 10~18 DEG C of ranges It is interior, and temperature is higher in early period, later stage bacteria cover diameter is grown to 2~3cm, and temperature is reduced;Relative air humidity is maintained at 89% ~91%, the later stage suitably reduces humidity;Intensity of illumination suitably increases illumination with 500~1000lx, later stage;
9) it harvests:When cap is open and flat, spore not yet launches, bacteria cover diameter it is consistent with stem or slightly smaller than stem when it is necessary to timely Harvesting;
Three-stage culture is used in the step 6) when bacteria:Initial stage room temperature is with 22 DEG C~24 DEG C, after the material temperature in bacterium bag rises, Room temperature should be turned down to 18 DEG C~22 DEG C, and mycelia covers with rear room temperature and is suitably turned up, and in the entire bacteria stage, material temperature does not all exceed 28 DEG C, and in the bacteria stage, culturing room remains dark, and relative air humidity controls within 70%, and frequent ventilation.
2. according to claim 1 a kind of using Eucalyptus urophylla-grandis as the method for planting almond abalone mushroom of raw material, which is characterized in that the step It is rapid 2) in bacterium bag specification be 17*35cm, and wet feed weighs 1100~1200 grams in bag.
3. according to claim 1 a kind of using Eucalyptus urophylla-grandis as the method for planting almond abalone mushroom of raw material, which is characterized in that the step It is rapid 2) can also be using bacterium bottle bottle, and bacterium bottle specification be 1100 milliliters, wet feed weighs 650~680 grams in bottle.
4. according to claim 1 a kind of using Eucalyptus urophylla-grandis as the method for planting almond abalone mushroom of raw material, which is characterized in that the step It is rapid 6) in carry out mycelium stimulation after the completion of bacteria:The old strain in bottleneck surface layer is scraped off with mushroom culturing device, and bottleneck charge level is made to flatten, bottle after mycelium stimulation Son, which stands upside down, to be placed, and mycelia is allowed to restore.
5. according to claim 1 a kind of using Eucalyptus urophylla-grandis as the method for planting almond abalone mushroom of raw material, which is characterized in that in step 8) in that inverted bacterium bottle is upright, temperature, relative air humidity and intensity of illumination are identical as bacterium bag.
CN201610041440.0A 2016-01-22 2016-01-22 It is a kind of using Eucalyptus urophylla-grandis as the method for planting almond abalone mushroom of raw material Active CN105580641B (en)

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CN106171509A (en) * 2016-06-30 2016-12-07 广西仁泰生物科技有限公司 A kind of method utilizing Eucalyptus waste material and Caulis et Folium Oryzae to produce Volvariella volvacea (Bull.Ex Franch.) Singer.

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